TCF20 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015471403.1	XP_015326889.1	transcription factor 20 isoform X6
XM_005207396.2	XP_005207453.1	transcription factor 20 isoform X4
XM_015471399.2	XP_015326885.1	transcription factor 20 isoform X1
XM_015471400.2	XP_015326886.1	transcription factor 20 isoform X2
XM_005207396.3	XP_005207453.1	transcription factor 20 isoform X4
XM_005207398.3	XP_005207455.1	transcription factor 20 isoform X5
XM_005207398.2	XP_005207455.1	transcription factor 20 isoform X5
XM_015471401.1	XP_015326887.1	transcription factor 20 isoform X3
NM_001192574.2	NP_001179503.2	transcription factor 20
XM_024991791.1	XP_024847559.1	transcription factor 20 isoform X1
XM_024991792.1	XP_024847560.1	transcription factor 20 isoform X1
XM_024991794.1	XP_024847562.1	transcription factor 20 isoform X6
XM_015471400.1	XP_015326886.1	transcription factor 20 isoform X2
XM_015471404.1	XP_015326890.1	transcription factor 20 isoform X6
XM_024991790.1	XP_024847558.1	transcription factor 20 isoform X1
XM_015471401.2	XP_015326887.1	transcription factor 20 isoform X3
XM_024991795.1	XP_024847563.1	transcription factor 20 isoform X7
XM_015471403.2	XP_015326889.1	transcription factor 20 isoform X9
NM_001192574.1	NP_001179503.1	transcription factor 20
XM_005207399.3	XP_005207456.1	transcription factor 20 isoform X7
XM_024991793.1	XP_024847561.1	transcription factor 20 isoform X2
XM_015471402.2	XP_015326888.1	transcription factor 20 isoform X9
XM_005207399.4	XP_005207456.1	transcription factor 20 isoform X10
XM_015471399.1	XP_015326885.1	transcription factor 20 isoform X1
XM_015471402.1	XP_015326888.1	transcription factor 20 isoform X6
XM_024991796.1	XP_024847564.1	transcription factor 20 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234399	XM_024991794.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234400	XM_024991795.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234401	XM_024991796.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa19982	NM_001192574.2 Latest version!	Bos taurus transcription factor 20 (TCF20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa36365	NM_001192574.1 Latest version!	Bos taurus transcription factor 20 (AR1) (TCF20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19982	XM_005207396.3 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19984	XM_005207398.3 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19985	XM_005207399.4 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90827	XM_015471399.2 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90827	XM_024991792.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90827	XM_024991791.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90827	XM_024991790.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90828	XM_024991793.1 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90828	XM_015471400.2 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90829	XM_015471401.2 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90830	XM_015471404.1 Latest version!	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90830	XM_015471402.2 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90830	XM_015471403.2 Latest version!	Bos taurus transcription factor 20 (TCF20), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa19982	XM_005207396.2	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19984	XM_005207398.2	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90828	XM_015471400.1	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90829	XM_015471401.1	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90830	XM_015471403.1	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19985	XM_005207399.3	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90827	XM_015471399.1	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90830	XM_015471402.1	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

TCF20 ( XM_024991794.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847562.1 Bos taurus transcription factor 20 (TCF20), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa234399
Clone ID Related Accession (Same CDS sequence)	XM_024991794.1
Accession Version	XM_024991794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA ATCAAGAACA AGCGTCCACC 
TGTTCAGGTA GCCTCATCTA TGTGGCCTCA GCCACCAGAA GAGTCTGCGC CTTTGCTCAG 
TGGGGCCAGT GGGGCTCATT TCACAAATGT GATTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847562.1
CDS	121..5922
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMNQEQASTCSGSLIYVASATRRVCAFAQWGQWGSFHKCDSC

Target ORF information:

RefSeq Version	XM_024991794.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991794.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA ATCAAGAACA AGCGTCCACC 
TGTTCAGGTA GCCTCATCTA TGTGGCCTCA GCCACCAGAA GAGTCTGCGC CTTTGCTCAG 
TGGGGCCAGT GGGGCTCATT TCACAAATGT GATTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991795.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847563.1 Bos taurus transcription factor 20 (TCF20), transcript variant X11, mRNA.

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CloneID	OBa234400
Clone ID Related Accession (Same CDS sequence)	XM_024991795.1
Accession Version	XM_024991795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG TGTTCTTGTT GGACATGGAG 
GTTCCGGTTA GTTTCCTGTC TGTATTCTTC AGTCGCTCAG TCCTCTCTGT CTCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847563.1
CDS	121..5880
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMVFLLDMEVPVSFLSVFFSRSVLSVSL

Target ORF information:

RefSeq Version	XM_024991795.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991795.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG TGTTCTTGTT GGACATGGAG 
GTTCCGGTTA GTTTCCTGTC TGTATTCTTC AGTCGCTCAG TCCTCTCTGT CTCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991796.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847564.1 Bos taurus transcription factor 20 (TCF20), transcript variant X12, mRNA.

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CloneID	OBa234401
Clone ID Related Accession (Same CDS sequence)	XM_024991796.1
Accession Version	XM_024991796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA ATCAAGAACA AGCGTCCACC 
TGTTCAGAAC GAGCACGCAC GGGCAGCTGC ATCACACGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847564.1
CDS	121..5862
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMNQEQASTCSERARTGSCITR

Target ORF information:

RefSeq Version	XM_024991796.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991796.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA ATCAAGAACA AGCGTCCACC 
TGTTCAGAAC GAGCACGCAC GGGCAGCTGC ATCACACGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( NM_001192574.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179503.2 Bos taurus transcription factor 20 (TCF20), mRNA.

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CloneID	OBa19982
Clone ID Related Accession (Same CDS sequence)	XM_005207396.2 , XM_005207396.3 , NM_001192574.2
Accession Version	NM_001192574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	transcription factor 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On Jun 30, 2017 this sequence version replaced NM_001192574.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN549117.1, SRR5571254.52107.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179503.2
CDS	1..5907
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	NM_001192574.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192574.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( NM_001192574.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179503.1 Bos taurus transcription factor 20 (AR1) (TCF20), mRNA.

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CloneID	OBa36365
Clone ID Related Accession (Same CDS sequence)	NM_001192574.1
Accession Version	NM_001192574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	transcription factor 20
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03052909.1 and AAFC03026338.1. On Jul 17, 2010 this sequence version replaced XM_601446.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN549117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02403652, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTACTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179503.1
CDS	1..5907
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPTSIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	NM_001192574.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192574.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTACTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207396.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207453.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.

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CloneID	OBa19982
Clone ID Related Accession (Same CDS sequence)	XM_005207396.2 , XM_005207396.3 , NM_001192574.2
Accession Version	XM_005207396.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005207396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207453.1
CDS	129..6035
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_005207396.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207396.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207396.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207453.1 Bos taurus transcription factor 20 (TCF20), transcript variant X8, mRNA.

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CloneID	OBa19982
Clone ID Related Accession (Same CDS sequence)	XM_005207396.2 , XM_005207396.3 , NM_001192574.2
Accession Version	XM_005207396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207453.1
CDS	121..6027
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_005207396.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207396.3

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207398.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207455.1 Bos taurus transcription factor 20 (TCF20), transcript variant X9, mRNA.

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CloneID	OBa19984
Clone ID Related Accession (Same CDS sequence)	XM_005207398.3 , XM_005207398.2
Accession Version	XM_005207398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207455.1
CDS	121..5961
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_005207398.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207398.3

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207398.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207455.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa19984
Clone ID Related Accession (Same CDS sequence)	XM_005207398.3 , XM_005207398.2
Accession Version	XM_005207398.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005207398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207455.1
CDS	129..5969
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_005207398.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207398.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207399.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207456.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa19985
Clone ID Related Accession (Same CDS sequence)	XM_005207399.3 , XM_005207399.4
Accession Version	XM_005207399.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207456.1
CDS	129..5810
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREM

Target ORF information:

RefSeq Version	XM_005207399.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207399.3

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005207399.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207456.1 Bos taurus transcription factor 20 (TCF20), transcript variant X16, mRNA.

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CloneID	OBa19985
Clone ID Related Accession (Same CDS sequence)	XM_005207399.3 , XM_005207399.4
Accession Version	XM_005207399.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207399.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207456.1
CDS	121..5802
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREM

Target ORF information:

RefSeq Version	XM_005207399.4
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207399.4

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471399.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326885.1 Bos taurus transcription factor 20 (TCF20), transcript variant X1, mRNA.

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CloneID	OBa90827
Clone ID Related Accession (Same CDS sequence)	XM_015471399.2 , XM_024991791.1 , XM_024991792.1 , XM_024991790.1 , XM_015471399.1
Accession Version	XM_015471399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326885.1
CDS	121..6147
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015471399.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471399.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991791.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847559.1 Bos taurus transcription factor 20 (TCF20), transcript variant X3, mRNA.

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CloneID	OBa90827
Clone ID Related Accession (Same CDS sequence)	XM_015471399.2 , XM_024991791.1 , XM_024991792.1 , XM_024991790.1 , XM_015471399.1
Accession Version	XM_024991791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847559.1
CDS	53..6079
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_024991791.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991791.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991792.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847560.1 Bos taurus transcription factor 20 (TCF20), transcript variant X4, mRNA.

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CloneID	OBa90827
Clone ID Related Accession (Same CDS sequence)	XM_015471399.2 , XM_024991791.1 , XM_024991792.1 , XM_024991790.1 , XM_015471399.1
Accession Version	XM_024991792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847560.1
CDS	121..6147
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_024991792.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991792.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847558.1 Bos taurus transcription factor 20 (TCF20), transcript variant X2, mRNA.

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CloneID	OBa90827
Clone ID Related Accession (Same CDS sequence)	XM_015471399.2 , XM_024991791.1 , XM_024991792.1 , XM_024991790.1 , XM_015471399.1
Accession Version	XM_024991790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847558.1
CDS	52..6078
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_024991790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991790.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471399.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326885.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

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CloneID	OBa90827
Clone ID Related Accession (Same CDS sequence)	XM_015471399.2 , XM_024991791.1 , XM_024991792.1 , XM_024991790.1 , XM_015471399.1
Accession Version	XM_015471399.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326885.1
CDS	129..6155
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPVPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015471399.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471399.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGCCTCCCC TCCCATGCCC TGTCCCCCCC TTGCAGAACA AGACAGCGAA AGGCAGCCTC 
AGCACAGAGC AGTCGGAGCG GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471400.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326886.1 Bos taurus transcription factor 20 (TCF20), transcript variant X5, mRNA.

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CloneID	OBa90828
Clone ID Related Accession (Same CDS sequence)	XM_015471400.2 , XM_015471400.1 , XM_024991793.1
Accession Version	XM_015471400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326886.1
CDS	121..6114
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015471400.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471400.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471400.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326886.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.

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CloneID	OBa90828
Clone ID Related Accession (Same CDS sequence)	XM_015471400.2 , XM_015471400.1 , XM_024991793.1
Accession Version	XM_015471400.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326886.1
CDS	129..6122
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_015471400.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471400.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_024991793.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847561.1 Bos taurus transcription factor 20 (TCF20), transcript variant X6, mRNA.

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CloneID	OBa90828
Clone ID Related Accession (Same CDS sequence)	XM_015471400.2 , XM_015471400.1 , XM_024991793.1
Accession Version	XM_024991793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847561.1
CDS	121..6114
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_024991793.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991793.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGAACAAGA CAGCGAAAGG CAGCCTCAGC ACAGAGCAGT CGGAGCGGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471401.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326887.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.

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CloneID	OBa90829
Clone ID Related Accession (Same CDS sequence)	XM_015471401.1 , XM_015471401.2
Accession Version	XM_015471401.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326887.1
CDS	129..6089
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_015471401.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471401.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471401.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326887.1 Bos taurus transcription factor 20 (TCF20), transcript variant X7, mRNA.

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CloneID	OBa90829
Clone ID Related Accession (Same CDS sequence)	XM_015471401.1 , XM_015471401.2
Accession Version	XM_015471401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326887.1
CDS	121..6081
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMEAWPETLLLDGVHDVVSEWRLEIDSPSCRQTPVTAETHAEKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_015471401.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471401.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGG AGGCCTGGCC TGAAACGCTG 
CTGCTGGATG GCGTCCACGA TGTGGTCAGC GAGTGGCGGT TAGAAATTGA CAGCCCGTCC 
TGCCGCCAGA CTCCAGTAAC CGCAGAGACA CACGCTGAGA AATGTTCCCA CTGCCAGGAG 
GCAGGCGCCA CCTTGGGCTG CTACAACAAA GGCTGCTCCT TCCGATACCA TTACCCGTGT 
GCCATTGATG CAGATTGTTT GCTCCACGAG GAGAACTTCT CGGTGAGGTG CCCCAAGCAC 
AAGGTGAGAC TGTGGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471403.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326889.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X8, mRNA.

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CloneID	OBa90830
Clone ID Related Accession (Same CDS sequence)	XM_015471403.1 , XM_015471404.1 , XM_015471403.2 , XM_015471402.2 , XM_015471402.1
Accession Version	XM_015471403.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326889.1
CDS	129..5840
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMIVCSTRRTSR

Target ORF information:

RefSeq Version	XM_015471403.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471403.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471404.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326890.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X9, mRNA.

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CloneID	OBa90830
Clone ID Related Accession (Same CDS sequence)	XM_015471403.1 , XM_015471404.1 , XM_015471403.2 , XM_015471402.2 , XM_015471402.1
Accession Version	XM_015471404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326890.1
CDS	129..5840
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMIVCSTRRTSR

Target ORF information:

RefSeq Version	XM_015471404.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471404.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471403.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326889.1 Bos taurus transcription factor 20 (TCF20), transcript variant X14, mRNA.

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CloneID	OBa90830
Clone ID Related Accession (Same CDS sequence)	XM_015471403.1 , XM_015471404.1 , XM_015471403.2 , XM_015471402.2 , XM_015471402.1
Accession Version	XM_015471403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326889.1
CDS	121..5832
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMIVCSTRRTSR

Target ORF information:

RefSeq Version	XM_015471403.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471403.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471402.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326888.1 Bos taurus transcription factor 20 (TCF20), transcript variant X13, mRNA.

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CloneID	OBa90830
Clone ID Related Accession (Same CDS sequence)	XM_015471403.1 , XM_015471404.1 , XM_015471403.2 , XM_015471402.2 , XM_015471402.1
Accession Version	XM_015471402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326888.1
CDS	121..5832
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMIVCSTRRTSR

Target ORF information:

RefSeq Version	XM_015471402.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (TCF20), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471402.2

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_015471402.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326888.1 Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X7, mRNA.

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CloneID	OBa90830
Clone ID Related Accession (Same CDS sequence)	XM_015471403.1 , XM_015471404.1 , XM_015471403.2 , XM_015471402.2 , XM_015471402.1
Accession Version	XM_015471402.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription factor 20 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326888.1
CDS	129..5840
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRIEEFSPRQAQMFQNFGGAGGGSSGSGGGGGGGRRAAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVATGTPQPPPRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSALGGVSHYSQDYTGPFSPGSAQYQQQPSSQQQQQQQQVQQLRQQLYQSHQPLPQAASQPASGASHLQPMQRPSTLPASAAGYQLRVGQFGQHYQSSATAASSSFPSPQRFSQSGQSYDGSYSVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPPGTQQGQQQQQQQQQQQQQQQQQHPPQHVMQYSNAATKLPLQSQVGQYSQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLTQSGENLQCGQGNVPMGSRNRILQLMPQLSPTPSMMPSPNSHTAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSSKKTDSCPNSEGSSQAEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEAPRLSTSPAVREETASPGAKDTPLSSEGNPKVNEKTVGVIVSREAMAGRVEKPGGQDKGSQEEDPAATQRPPSTGGAKEASHASLPQPEPPGGGSKGNKSGDGNSNHNGEGNGQTGHPAGGSGFTGRTEPSKSPGSLRYSYKDSFGSAVPRNISSFAQYPTGQDKGDFTSHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSSQEHQAMAGSLEGATRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSGTAPEMKQINLADYPIPRKFEIEPPSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMGADTRLGRNERLNPSLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPASIKHESYRGNASPGAATHDSISDYGPQDSRPTPMRRVPGRVGSREGMRGRSPSQYHDFSEKLKMSPGRSRGPGGDPHHMSPHMTFSERANRSSLHAPFSPNSESLASAYHTNTRAHAYGDPSAGLNSQLHYKRQMYQQQQEEYKDWGGSSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPMGTYHDPSGQDGGRCLMSSDGLSNKGIELKHGSQKLQQESCWDLSRQTSPAKSSGPPGMSNQKRYGPPHETDGHGLAESTQSSKPSNVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSNTEDKGRLLHPSKEGTDKAYNSYAHLSHSQEIKSIPKRESSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSGAVPDTELEKRKGEVIAELACPAGQELSGEKPLARSSEEWRGGGDDKVKTETHPDTVAAGKEPPGAMASTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGTLAPEANPKAEEKESDAVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAIPIVEPQEPEIKLKYATQPLDKTDAKNKSFFPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKVLPASSFVLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRAAETQSKVKVRHKSASNGSKTDTEEEEEQQQQKEQRSLAAHPRFKRRHRSEDCAGGPRSLSRGLPCKKATTEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMIVCSTRRTSR

Target ORF information:

RefSeq Version	XM_015471402.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 20 (AR1) (TCF20), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471402.1

ORF Insert Sequence:

ATGCAGTCCT TCCGGGAGCA AAGCAGTTAC CACGGGAACC AGCAGAGCTA CCCCCAGGAG 
GTGCACGGCT CATCCCGGAT AGAGGAGTTC AGCCCTCGCC AGGCCCAGAT GTTCCAGAAT 
TTTGGGGGTG CTGGTGGTGG CAGCAGCGGC AGCGGCGGCG GCGGCGGTGG TGGACGACGA 
GCAGCGGCGG CGGCGGCGGC CGCGATGGCC AGCGAGACCT CCGGCCATCA GGGCTACCAG 
GGCTTCAGGA AGGAGGCTGG AGACTTTTAC TACATGGCAG GCAACAAGGA CCCCGTGGCC 
ACAGGAACCC CGCAGCCTCC TCCGCGAAGG CCTTCCGGGC CGGTGCAGAG CTACGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGCAGTGAGG GTCACGTGGG CCAGTTTCAA 
GCACAGCACT CTGCCCTTGG CGGTGTGTCT CATTACTCGC AGGATTACAC GGGGCCGTTC 
TCTCCGGGGA GCGCTCAGTA CCAGCAGCAG CCTTCCAGCC AGCAGCAGCA GCAGCAGCAG 
CAAGTGCAGC AGCTGCGGCA GCAGCTCTAC CAGTCCCATC AGCCCCTGCC GCAGGCTGCC 
AGCCAGCCGG CGTCTGGCGC CTCGCACCTG CAGCCCATGC AGCGGCCCTC AACTCTGCCG 
GCCTCGGCCG CCGGCTACCA GCTGAGAGTG GGGCAGTTCG GCCAGCACTA CCAGTCTTCT 
GCCACCGCCG CCTCCTCCTC CTTCCCATCA CCGCAGCGTT TCAGCCAGTC TGGGCAGAGC 
TATGACGGCA GTTATAGTGT GAACGCCGGA TCACAGTACG AAGGGCATAA CGTGGGCTCC 
AACGCACAGG CTTATGGAAC ACAATCCAAT TACAGCTATC AGCCTCAATC TATGAAGAAT 
TTCGAACAGG CCAAGATTCC ACCAGGGACC CAGCAGGGGC AGCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CACCCCCCTC AGCACGTGAT GCAGTATTCC 
AACGCTGCCA CCAAGCTGCC CCTGCAAAGC CAGGTCGGGC AGTACAGCCA GCCCGAGGTT 
CCCGTGAGGT CCCCCATGCA GTTTCACCAG AACTTCAGCC CCATCTCTAA CCCTTCCCCA 
GCTGCCTCCG TGGTTCAGTC TCCAAGCTGT AGCTCTACCC CGTCTCCTCT CACACAGAGC 
GGGGAGAACC TCCAGTGTGG GCAAGGCAAT GTGCCAATGG GCTCCAGAAA CAGAATCCTG 
CAGTTAATGC CTCAGCTCAG CCCAACCCCG TCGATGATGC CCAGTCCCAA CTCTCACACT 
GCGGGCTTCA AAGGGTTCGG GCTGGAAGGG GTGCCTGAAA AGCGGCTGAC CGATCCCGGC 
TTGAGTAGTT TGAGTGCCCT GAGTACGCAG GTGGCCAACC TTCCTAATAC GGTTCAGCAC 
ATGCTACTTT CCGACGCCCT GACCCCTCAG AAGAAGACCT CCAAGAGGCC TTCCTCGTCA 
TCCAAGAAAA CAGACAGCTG CCCAAACTCG GAAGGCTCTT CACAGGCTGA AGAACAACTG 
AAGTCCCCGA TGGCAGAGTC GCTAGATGGA GGCTGCTCCA GCAGTTCTGA GGATCAAGGC 
GAGAGGGTGA GGCAGCTGAG TGGCCAGAGC ACAAGCTCGG ACACCACCTA CAAGGGCGGG 
GCCTCGGAAA AGGCAGGCTC CTCACCAGCA CAGGGTGCTC AGAACGAAGC CCCCCGACTC 
AGCACCAGTC CTGCAGTCCG AGAAGAGACC GCCTCGCCGG GTGCCAAGGA CACACCACTG 
TCATCTGAGG GCAACCCAAA AGTCAACGAG AAGACAGTCG GGGTGATTGT CTCCCGGGAA 
GCTATGGCAG GCCGGGTGGA AAAGCCTGGT GGGCAGGACA AAGGCTCCCA GGAAGAGGAT 
CCTGCAGCCA CACAGAGGCC ACCCAGCACC GGGGGGGCAA AGGAAGCCAG CCACGCGTCA 
CTTCCACAGC CAGAGCCTCC GGGAGGAGGC AGTAAAGGAA ACAAGAGTGG AGACGGTAAC 
TCCAACCACA ATGGGGAAGG GAATGGCCAG ACTGGGCACC CTGCAGGGGG CTCTGGTTTC 
ACAGGCAGGA CTGAGCCCAG CAAATCTCCT GGAAGCCTGC GCTATAGTTA CAAAGACAGT 
TTTGGGTCAG CTGTGCCGAG AAACATTAGC AGCTTTGCTC AGTATCCTAC AGGACAAGAT 
AAAGGGGACT TCACCAGCCA CGGGGAGCGA AAGGGTAGAA ATGAGAAGTT CCCCAGCCTC 
CTGCAGGAGG TGCTTCAGGG TTACCACCAC CACCCCGACA GGAGGTACTC CCGGAGTTCT 
CAGGAGCACC AGGCCATGGC TGGCAGCCTC GAAGGAGCCA CAAGGCCTAA TGTCTTAGTG 
AGTCAGACCA ATGAGTTAGC TAGCAGGGGC CTTCTGAACA AAAGCATTGG GTCCCTGTTA 
GAAAACCCCC ACTGGGGCCC CTGGGAAAGA AAGTCAAGTG GCACAGCTCC TGAGATGAAA 
CAGATCAATT TGGCCGACTA CCCAATTCCC AGAAAGTTTG AAATAGAGCC TCCATCGTCA 
GCCCATGAGC CCGGGGGTTC CCTCTCTGAG AGAAGATCAG TCATCTGTGA CATTTCTCCA 
CTAAGACAGA TTGTCAGAGA CCCGGGGGCT CACTCGCTAG GACACATGGG CGCCGACACC 
AGACTTGGGA GGAATGAGCG TCTCAATCCC AGTTTAAGTC AGTCAGTCAT TCTTCCAGGT 
GGGCTGGTGT CCATGGAAAC AAAGCTGAAA TCCCAGAGTG GGCAGATAAA AGAGGAAGAC 
TTTGAACAGT CCAAGTCCCA AGCTAGTTTC AACAACAAGA AATCGGGAGA CCACTGCCAC 
CCTGCTAGCA TCAAGCACGA GTCTTACCGA GGCAATGCCA GCCCCGGAGC GGCGACCCAT 
GATTCCATCT CAGACTACGG CCCGCAGGAC AGCAGACCCA CACCAATGCG GCGAGTCCCC 
GGCAGAGTTG GCAGTCGGGA GGGCATGAGG GGTCGGTCCC CTTCTCAGTA TCATGACTTT 
TCAGAAAAGT TGAAGATGTC CCCTGGGAGG AGCAGAGGCC CAGGGGGCGA CCCTCATCAC 
ATGAGCCCAC ACATGACCTT CTCCGAGAGG GCCAACAGGA GTTCTTTGCA TGCTCCCTTT 
TCTCCCAATT CGGAAAGCCT GGCCTCTGCT TATCACACAA ACACGCGTGC TCATGCTTAT 
GGGGACCCCA GTGCAGGTTT GAATTCCCAG CTCCATTACA AGAGACAGAT GTACCAACAG 
CAGCAAGAGG AATATAAGGA CTGGGGTGGC AGTTCTGCTC AGGGAGTGAT CGCTGCGGCT 
CAGCATAGGC AGGAGGGACC CCGCAAGAGT CCACGTCAGC AGCAGTTTCT CGACCGAGTC 
CGGAGCCCCC TGAAGAATGA CAAAGATGGC ATGATGTATG GCCCACCGAT GGGGACGTAC 
CATGACCCCA GCGGTCAGGA CGGTGGGCGC TGCCTCATGT CTAGTGATGG TCTTTCTAAC 
AAAGGCATTG AACTGAAGCA CGGCTCCCAG AAGTTACAGC AAGAATCTTG TTGGGATCTC 
TCTCGGCAGA CTTCTCCAGC CAAAAGCAGC GGCCCCCCAG GAATGTCCAA TCAGAAAAGG 
TACGGACCAC CCCATGAGAC CGATGGACAC GGGCTCGCAG AGTCTACGCA GTCATCCAAA 
CCGAGTAACG TTATGCTGAG ACTTCCAGGT CAAGAGGATC ATTCTTCTCA AAACCCCTTA 
ATCATGAGGA GGCGTGTCCG TTCTTTTATC TCTCCCATTC CCAGTAAGAG ACAGTCACAG 
GATGTGAAGA ACAGTAACAC TGAAGATAAA GGGCGCCTCC TTCACCCGTC AAAAGAAGGC 
ACTGACAAAG CGTACAATTC CTATGCCCAT CTTTCTCACA GTCAGGAGAT CAAGTCCATC 
CCTAAGAGGG AGTCCTCCAA GGACCTTCCA AGTCCAGATA GTAGGAACTG CCCTGCGGTC 
ACCCTTACAA GTCCTGCTAA GACCAAAATC CTGCCCCCAA GGAAAGGCCG GGGGTTGAAA 
CTGGAAGCTA TCGTCCAGAA GATCACATCC CCAAACATTA GGAGGAGCGC GTCCTCGAAC 
AGCGCAGAGG CTGGGGGAGA CACAGTTACT CTTGACGACA TCCTGTCTTT GAAAAGCGGC 
CCTCCGGAAG GTGGGAGCGG TGCCGTTCCA GACACTGAGC TGGAGAAGAG AAAAGGCGAG 
GTGATAGCTG AGCTGGCCTG TCCCGCAGGC CAGGAGCTGA GCGGAGAAAA GCCTCTGGCC 
CGGTCTTCGG AGGAGTGGCG CGGTGGTGGG GACGACAAGG TGAAGACGGA GACGCACCCT 
GACACGGTCG CCGCTGGGAA GGAGCCCCCT GGTGCCATGG CATCCACAAC CTCACAGAAG 
CCTGGGAGTA ACCAAGGGAG ACCAGACGGG TCCCTGGGCG GGACAGCACC TTTAATCTTT 
CCTGACTCAA AGAATGTACC TCCAGTGGGC ACGTTGGCTC CCGAGGCAAA CCCCAAGGCT 
GAAGAGAAAG AGAGCGATGC CGTGACGATT TCCCCCAAAC AGGAGGGCTT CCCCCCGAAG 
GGTTACTTCC CCTCAGGAAA GAAGAAAGGG AGACCCATTG GTAGTGTAAA TAAGCAGAAG 
AAACAACAGC AGCCCCCGCC GCCACCCCCT CAACCCCCTC AGATACCAGA GGGTTCTGCT 
GATGGAGAGC CAAAGCCAAA AAAGCAGAGG CAGCGGAGGG AGCGAAGGAA GCCTGGGGCG 
CAGCCCAGGA AGCGGAAAAC CAAACAGGCC ATTCCCATCG TAGAACCCCA AGAACCGGAG 
ATCAAGCTGA AGTACGCCAC CCAGCCCCTG GATAAAACCG ATGCCAAGAA CAAGTCTTTT 
TTCCCTTATA TCCACGTAGT AAATAAGTGT GAACTTGGAG CCGTGTGTAC AATCATCAAC 
GCCGAGGAGG AGGAGCAGAC CAAATTGGTG AGGGGGCGGA AGGGCCAGCG GTCCCTGACG 
CCTCCGCCCA GCAGCACCGA GAGCAAGGTT CTCCCAGCCT CGTCCTTCGT GCTGCAGGGC 
CCCGTGGTGA CAGAGTCTTC TGTGATGGGG CACCTGGTTT GCTGTCTGTG CGGCAAGTGG 
GCCAGTTACC GGAACATGGG CGACCTCTTT GGACCCTTTT ACCCCCAAGA TTATGCAGCC 
ACTCTCCCCA AGAATCCACC TCCCAAGAGG GCCGCGGAAA CGCAGAGCAA GGTGAAGGTC 
CGGCACAAAA GCGCTTCGAA CGGCTCCAAG ACGGACACTG AGGAGGAGGA AGAGCAGCAG 
CAGCAGAAGG AGCAGAGGAG CCTGGCCGCG CACCCCAGGT TTAAGCGGCG ACACCGCTCG 
GAAGACTGTG CCGGAGGCCC CCGGTCCCTG TCCAGGGGGC TCCCTTGTAA AAAAGCCACC 
ACCGAGGGCA GCAGCGAAAA GACTGTTTTG GACTCAAAGC CCTCCGTGCC CACCACTTCA 
GAAGGTGGCC CCGAGTTGGA GTTACAAATC CCTGAACTAC CTCTCGACAG CAATGAATTT 
TGGGTCCACG AGGGTTGTAT TCTCTGGGCC AATGGAATCT ACCTGGTCTG TGGCCGGCTC 
TATGGCCTGC AGGAAGCGCT GGAAATAGCC AGAGAGATGA TTGTTTGCTC CACGAGGAGA 
ACTTCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TCF20
Entrez Gene ID	523151
Full Name	transcription factor 20
General protein information	Preferred Names transcription factor 20 Names transcription factor 20
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	TCF20	XP_001107318.2
Canis lupus familiaris (dog)	TCF20	XP_857454.2
Rattus norvegicus (Norway rat)	Tcf20	NP_001124046.1
Homo sapiens (human)	TCF20	NP_005641.1
Pan troglodytes (chimpanzee)	TCF20	XP_003317325.1
Mus musculus (house mouse)	Tcf20	NP_001107612.1
Gallus gallus (chicken)	TCF20	XP_004937744.1
Xenopus tropicalis (tropical clawed frog)	tcf20	XP_004913850.1
Bos taurus (cattle)	TCF20	NP_001179503.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TCF20 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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