TIMELESS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMELESS
Entrez Gene ID	522884
Full Name	timeless circadian regulator
General protein information	Preferred Names timeless circadian regulator Names protein timeless homolog timeless circadian clock timeless homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_010805186.2	XP_010803488.1	protein timeless homolog isoform X1
NM_001102071.2	NP_001095541.2	protein timeless homolog
XM_024991789.1	XP_024847557.1	protein timeless homolog isoform X1
XM_024991788.1	XP_024847556.1	protein timeless homolog isoform X1
XM_010805186.3	XP_010803488.1	protein timeless homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMELESS	NP_003911.2
Canis lupus familiaris (dog)	TIMELESS	XP_538233.3
Pan troglodytes (chimpanzee)	TIMELESS	XP_509535.2
	LOC712835	XP_002808027.1
Danio rerio (zebrafish)	timeless	NP_001265529.1
Xenopus tropicalis (tropical clawed frog)	timeless	XP_002934575.1
Bos taurus (cattle)	TIMELESS	NP_001095541.2
Mus musculus (house mouse)	Timeless	NP_035719.1
Rattus norvegicus (Norway rat)	Timeless	NP_112630.1
Drosophila melanogaster (fruit fly)	timeout	NP_524341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010787	XP_001689129.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TIMELESS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMELESS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00005	NM_001102071.2 Latest version!	Bos taurus timeless circadian regulator (TIMELESS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa00005	XM_024991789.1 Latest version!	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa00005	XM_024991788.1 Latest version!	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa00005	XM_010805186.3 Latest version!	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
View Old Accession Versions >>
OBa00005	XM_010805186.2	Bos taurus timeless circadian clock (TIMELESS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMELESS ( NM_001102071.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095541.2 Bos taurus timeless circadian regulator (TIMELESS), mRNA.

Price & Availability↑

CloneID	OBa00005
Clone ID Related Accession (Same CDS sequence)	XM_010805186.2 , NM_001102071.2 , XM_024991789.1 , XM_024991788.1 , XM_010805186.3
Accession Version	NM_001102071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	protein timeless homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000005.1. On Feb 15, 2012 this sequence version replaced NM_001102071.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095541.2
CDS	498..4139
Translation	MMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGSAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKEPSFRHHFLQVLAYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNVLHVPADLEQEKRIDDDASVHDRLLWAIHLSGLDDLLLFLASSPAEQQWSLHVLEIISLMFRDQNPEQLAGVGQGRLAQERRTDVAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIIQGLKSIGERDLVFHKGLHNLQNYTSDLGKQPRRVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWSLAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAVSWARRMHLALKAYQELLATVNEMDVCPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRTRGNLMVQNKRKKRKKKKKALDQSVASGNAPYSPEELEAMWPSLAEQLQCRAQDSELSLDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALSLLRSAREVWPERDVFGAQDISPEEEIQLLKQILLAPLPRQQGLEEQGAEEEDEEEEEEEELEVVQVSEREFNFLDYLKRFANSTVLRAYLLLLRSYKQNSAHTNHCVVKMLHRLAHDLKMEALLFQLSLFYLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGTLDGGSSSRRLPGWSPEEEAQLRELYLAHKDVEGQDVVDTILAHLKTAPRTRRQVIHHLVRLGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGNIMKNITAKRSRARIVDKLLALGLVAERRELYKKRRKKLAPLSLPNEEESLHDFCQEDLEEEENVPEKDNEEDEEKEGSEAEQPQGGSIISAEYLGQSLHQEGFSAPLLWLQNCLIRAADDREEAGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLNPTQLRRMSASLSQAEEEEKLQLGLESKVPGEPGLEEEHQQEEEHQQEEEHQKEHRAHALRTLMLARKKEAGLVSPEEEGTSGGKEQLIMAPKKRQLLDSDEEQEEDKAPELGVPGIRKKKRFQIEDDDESD

Target ORF information:

RefSeq Version	NM_001102071.2
Organism	Bos taurus(cattle)
Definition	Bos taurus timeless circadian regulator (TIMELESS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102071.2

ORF Insert Sequence:

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( XM_010805186.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803488.1 Bos taurus timeless circadian clock (TIMELESS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa00005
Clone ID Related Accession (Same CDS sequence)	XM_010805186.2 , NM_001102071.2 , XM_024991789.1 , XM_024991788.1 , XM_010805186.3
Accession Version	XM_010805186.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803488.1
CDS	134..3775
Translation	MMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGSAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKEPSFRHHFLQVLAYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNVLHVPADLEQEKRIDDDASVHDRLLWAIHLSGLDDLLLFLASSPAEQQWSLHVLEIISLMFRDQNPEQLAGVGQGRLAQERRTDVAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIIQGLKSIGERDLVFHKGLHNLQNYTSDLGKQPRRVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWSLAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAVSWARRMHLALKAYQELLATVNEMDVCPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRTRGNLMVQNKRKKRKKKKKALDQSVASGNAPYSPEELEAMWPSLAEQLQCRAQDSELSLDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALSLLRSAREVWPERDVFGAQDISPEEEIQLLKQILLAPLPRQQGLEEQGAEEEDEEEEEEEELEVVQVSEREFNFLDYLKRFANSTVLRAYLLLLRSYKQNSAHTNHCVVKMLHRLAHDLKMEALLFQLSLFYLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGTLDGGSSSRRLPGWSPEEEAQLRELYLAHKDVEGQDVVDTILAHLKTAPRTRRQVIHHLVRLGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGNIMKNITAKRSRARIVDKLLALGLVAERRELYKKRRKKLAPLSLPNEEESLHDFCQEDLEEEENVPEKDNEEDEEKEGSEAEQPQGGSIISAEYLGQSLHQEGFSAPLLWLQNCLIRAADDREEAGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLNPTQLRRMSASLSQAEEEEKLQLGLESKVPGEPGLEEEHQQEEEHQQEEEHQKEHRAHALRTLMLARKKEAGLVSPEEEGTSGGKEQLIMAPKKRQLLDSDEEQEEDKAPELGVPGIRKKKRFQIEDDDESD

Target ORF information:

RefSeq Version	XM_010805186.2
Organism	Bos taurus(cattle)
Definition	Bos taurus timeless circadian clock (TIMELESS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805186.2

ORF Insert Sequence:

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( XM_024991789.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847557.1 Bos taurus timeless circadian regulator (TIMELESS), transcript variant X3, mRNA.

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CloneID	OBa00005
Clone ID Related Accession (Same CDS sequence)	XM_010805186.2 , NM_001102071.2 , XM_024991789.1 , XM_024991788.1 , XM_010805186.3
Accession Version	XM_024991789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847557.1
CDS	197..3838
Translation	MMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGSAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKEPSFRHHFLQVLAYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNVLHVPADLEQEKRIDDDASVHDRLLWAIHLSGLDDLLLFLASSPAEQQWSLHVLEIISLMFRDQNPEQLAGVGQGRLAQERRTDVAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIIQGLKSIGERDLVFHKGLHNLQNYTSDLGKQPRRVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWSLAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAVSWARRMHLALKAYQELLATVNEMDVCPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRTRGNLMVQNKRKKRKKKKKALDQSVASGNAPYSPEELEAMWPSLAEQLQCRAQDSELSLDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALSLLRSAREVWPERDVFGAQDISPEEEIQLLKQILLAPLPRQQGLEEQGAEEEDEEEEEEEELEVVQVSEREFNFLDYLKRFANSTVLRAYLLLLRSYKQNSAHTNHCVVKMLHRLAHDLKMEALLFQLSLFYLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGTLDGGSSSRRLPGWSPEEEAQLRELYLAHKDVEGQDVVDTILAHLKTAPRTRRQVIHHLVRLGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGNIMKNITAKRSRARIVDKLLALGLVAERRELYKKRRKKLAPLSLPNEEESLHDFCQEDLEEEENVPEKDNEEDEEKEGSEAEQPQGGSIISAEYLGQSLHQEGFSAPLLWLQNCLIRAADDREEAGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLNPTQLRRMSASLSQAEEEEKLQLGLESKVPGEPGLEEEHQQEEEHQQEEEHQKEHRAHALRTLMLARKKEAGLVSPEEEGTSGGKEQLIMAPKKRQLLDSDEEQEEDKAPELGVPGIRKKKRFQIEDDDESD

Target ORF information:

RefSeq Version	XM_024991789.1
Organism	Bos taurus(cattle)
Definition	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991789.1

ORF Insert Sequence:

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( XM_024991788.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847556.1 Bos taurus timeless circadian regulator (TIMELESS), transcript variant X1, mRNA.

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CloneID	OBa00005
Clone ID Related Accession (Same CDS sequence)	XM_010805186.2 , NM_001102071.2 , XM_024991789.1 , XM_024991788.1 , XM_010805186.3
Accession Version	XM_024991788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847556.1
CDS	310..3951
Translation	MMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGSAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKEPSFRHHFLQVLAYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNVLHVPADLEQEKRIDDDASVHDRLLWAIHLSGLDDLLLFLASSPAEQQWSLHVLEIISLMFRDQNPEQLAGVGQGRLAQERRTDVAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIIQGLKSIGERDLVFHKGLHNLQNYTSDLGKQPRRVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWSLAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAVSWARRMHLALKAYQELLATVNEMDVCPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRTRGNLMVQNKRKKRKKKKKALDQSVASGNAPYSPEELEAMWPSLAEQLQCRAQDSELSLDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALSLLRSAREVWPERDVFGAQDISPEEEIQLLKQILLAPLPRQQGLEEQGAEEEDEEEEEEEELEVVQVSEREFNFLDYLKRFANSTVLRAYLLLLRSYKQNSAHTNHCVVKMLHRLAHDLKMEALLFQLSLFYLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGTLDGGSSSRRLPGWSPEEEAQLRELYLAHKDVEGQDVVDTILAHLKTAPRTRRQVIHHLVRLGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGNIMKNITAKRSRARIVDKLLALGLVAERRELYKKRRKKLAPLSLPNEEESLHDFCQEDLEEEENVPEKDNEEDEEKEGSEAEQPQGGSIISAEYLGQSLHQEGFSAPLLWLQNCLIRAADDREEAGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLNPTQLRRMSASLSQAEEEEKLQLGLESKVPGEPGLEEEHQQEEEHQQEEEHQKEHRAHALRTLMLARKKEAGLVSPEEEGTSGGKEQLIMAPKKRQLLDSDEEQEEDKAPELGVPGIRKKKRFQIEDDDESD

Target ORF information:

RefSeq Version	XM_024991788.1
Organism	Bos taurus(cattle)
Definition	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991788.1

ORF Insert Sequence:

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( XM_010805186.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803488.1 Bos taurus timeless circadian regulator (TIMELESS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa00005
Clone ID Related Accession (Same CDS sequence)	XM_010805186.2 , NM_001102071.2 , XM_024991789.1 , XM_024991788.1 , XM_010805186.3
Accession Version	XM_010805186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805186.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803488.1
CDS	192..3833
Translation	MMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGSAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKEPSFRHHFLQVLAYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNVLHVPADLEQEKRIDDDASVHDRLLWAIHLSGLDDLLLFLASSPAEQQWSLHVLEIISLMFRDQNPEQLAGVGQGRLAQERRTDVAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIIQGLKSIGERDLVFHKGLHNLQNYTSDLGKQPRRVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWSLAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAVSWARRMHLALKAYQELLATVNEMDVCPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRTRGNLMVQNKRKKRKKKKKALDQSVASGNAPYSPEELEAMWPSLAEQLQCRAQDSELSLDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALSLLRSAREVWPERDVFGAQDISPEEEIQLLKQILLAPLPRQQGLEEQGAEEEDEEEEEEEELEVVQVSEREFNFLDYLKRFANSTVLRAYLLLLRSYKQNSAHTNHCVVKMLHRLAHDLKMEALLFQLSLFYLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGTLDGGSSSRRLPGWSPEEEAQLRELYLAHKDVEGQDVVDTILAHLKTAPRTRRQVIHHLVRLGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGNIMKNITAKRSRARIVDKLLALGLVAERRELYKKRRKKLAPLSLPNEEESLHDFCQEDLEEEENVPEKDNEEDEEKEGSEAEQPQGGSIISAEYLGQSLHQEGFSAPLLWLQNCLIRAADDREEAGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLNPTQLRRMSASLSQAEEEEKLQLGLESKVPGEPGLEEEHQQEEEHQQEEEHQKEHRAHALRTLMLARKKEAGLVSPEEEGTSGGKEQLIMAPKKRQLLDSDEEQEEDKAPELGVPGIRKKKRFQIEDDDESD

Target ORF information:

RefSeq Version	XM_010805186.3
Organism	Bos taurus(cattle)
Definition	Bos taurus timeless circadian regulator (TIMELESS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805186.3

ORF Insert Sequence:

ATGATGAATT GTGAACTTCT TGCCACATGT AGTGCCCTTG GGTACTTGGA GGGAGACACT 
TACCACAAGG AGCCAGATTG CTTAGAGAGT GTGAAGGACT TGATCCGCTA CTTGAGGCAC 
GAGGATGAGA CACGGGACGT GCGGCAGCAG CTGGGATCGG CTCAGATCCT GCAGAGTGAC 
CTCCTGCCCA TCCTCACCCA GCATCGCCAG GACAAGCCTC TTTTTGATGC TGTTATCAGG 
CTGATGGTGA ACTTGACACA ACCAGCCTTG CTCTGTTTCG GCAGCGTGCC CAAGGAGCCC 
AGCTTCCGGC ACCACTTTCT GCAGGTGCTG GCATACTTGC AGGCTTACAA AGAGGCCTTT 
GCCAGTGAGA AGGCATTTGG GGTCCTCAGT GAAACTTTGT ATGAGCTGCT GCAGCTGGGC 
TGGGAGGAAC GCCAGGAGGA AGACAACTTG CTGATTGAGC GGATCCTGCT GCTGGTCAGA 
AACGTCCTCC ATGTCCCCGC CGACCTTGAG CAGGAGAAGC GGATTGACGA TGACGCCAGT 
GTGCACGACC GGCTTCTCTG GGCAATCCAC CTCAGTGGCC TGGATGACCT GCTCCTCTTC 
CTGGCCAGCT CACCTGCGGA GCAGCAGTGG AGCCTCCATG TGCTGGAGAT CATCTCCCTT 
ATGTTTCGTG ACCAGAACCC TGAGCAACTG GCAGGAGTAG GGCAGGGACG CTTGGCTCAG 
GAGCGGAGGA CAGATGTGGC AGAGCTGGAG GTCTTGCGCC AGCGAGAGAT GGCAGAAAAG 
AAGACTCGAG CCCTGCAGCG AGGCAACAGG CATTCTCGGT TTGGGGGTTC CTACATCATC 
CAGGGCTTGA AATCCATTGG GGAGAGGGAC CTTGTATTTC ATAAAGGTCT CCACAATCTC 
CAAAACTATA CTTCTGATTT GGGAAAGCAG CCCCGACGGG TGCCTAAACG TCGCCAGGCT 
GCCCGGGAGC TGTCCATCCA GCGCCGCTCT GCCCTCAACG TGAGACTCTT TCTTCGGGAC 
TTCTGCTCGG AGTTCCTGGA AAACTGTTAC AACCGGCTCA TGGGCTCGGT GAAGGACCAC 
CTGCTTCGGG AGAAGGCTCA ACAGCATGAC GAGACCTACT ACATGTGGTC CCTGGCTTTC 
TTCATGGCCT TCAACCGAGC TGCCTCCTTC CGGCCAGGCC TGGTTTCTGA GACGCTCAGT 
GTCCGCACCT TCCACTTCAT TGAGCAGAAC CTCACCAACT ACTATGAGAT GATGCTGACC 
GACCGCAAGG AAGCCGTCTC CTGGGCACGC CGGATGCACC TGGCTCTGAA GGCGTATCAG 
GAGCTGCTAG CCACAGTGAA TGAGATGGAT GTGTGCCCAG ACGAGGCTGT GAGGGAGAGC 
AGCCGCATCA TCAAGAACAA CATTTTCTAT GTGATGGAGT ACCGGGAGCT CTTCCTGGCT 
CTCTTTCGGA AGTTTGATGA GAGATGCCAG CCCCGCTCTT TCCTTCGTGA CCTGGTGGAA 
ACCACCCACC TCTTCCTCAA GATGTTGGAG CGGTTCTGTC GGACCCGTGG GAACCTGATG 
GTGCAGAACA AACGAAAGAA AAGAAAGAAG AAAAAGAAGG CCTTAGATCA GTCTGTTGCT 
TCTGGTAATG CCCCCTACAG CCCAGAGGAA CTGGAGGCCA TGTGGCCCTC CCTGGCCGAG 
CAGCTGCAGT GCCGTGCCCA GGATTCTGAG CTCAGTTTGG ACTCTGTGGT TCCCTTTGAT 
GCGGCCTCAG AGGTGCCAGT GGAAGAGCAG CGGGCAGAAG CCATGGTGCG GATCCAGGAC 
TGCCTCCTGG CTGGCCAGGC CCCGCAGGCC CTGAGCCTCC TGAGGTCTGC CCGGGAGGTG 
TGGCCAGAAA GAGATGTGTT TGGTGCTCAG GACATCTCCC CAGAGGAGGA GATCCAGCTA 
CTGAAGCAAA TCCTCTTGGC CCCGCTTCCC CGCCAGCAGG GTCTGGAGGA ACAAGGGGCG 
GAGGAAGAAG ACGAAGAAGA GGAGGAGGAG GAAGAGTTAG AAGTGGTCCA GGTGTCGGAG 
AGAGAATTTA ACTTTTTGGA CTACCTGAAA CGCTTCGCAA ACTCAACCGT CCTCCGAGCC 
TACCTGCTGC TGCTGCGGAG CTACAAGCAG AACAGCGCCC ACACCAACCA CTGTGTGGTC 
AAGATGCTGC ACCGGCTGGC CCATGATCTC AAAATGGAAG CCCTGCTTTT CCAGCTTTCC 
CTCTTCTACC TCTTCAATCG TCTGCTCAGC GATCCTGCTG CCGGGGCCTA CAAAGAGCTG 
GTGACTTTTG CCAAGTATAT CCTGGGCAAG TTCTTTGCTT TGGCTGCTGT CAACCAGAAA 
GCCTTTGTGG AGCTGCTGTT CTGGAAGAAC ACTGCTGTGG TTCGAGAGAT GACCGAGGGC 
TACGGCACCC TGGATGGCGG ATCTTCTAGC CGCAGACTCC CTGGATGGAG CCCAGAGGAG 
GAGGCCCAGC TTCGGGAACT GTATCTTGCC CATAAGGATG TGGAAGGTCA GGATGTGGTG 
GACACCATCT TGGCACACCT GAAAACTGCT CCTCGGACAC GCAGGCAGGT CATCCACCAT 
CTGGTGCGGC TGGGACTGGC TGACAGCGTC AAGGACTTCC AGAGGAAAGG AACCCAGATT 
GTGCTGTGGA CGGAGGATCA GGAGCTGGAG CTGCAGCGGC TTTTTGAGGA GTTTCAGGAC 
TCCGATGATG TCCTGGGTAA TATCATGAAG AACATTACAG CCAAACGCTC ACGGGCCCGA 
ATAGTGGATA AACTGCTGGC TCTGGGGCTG GTGGCTGAGC GGCGGGAGCT GTACAAGAAA 
CGCCGGAAGA AGCTGGCACC CTTGAGCTTG CCTAATGAAG AGGAATCCTT GCACGATTTT 
TGCCAGGAAG ATCTAGAAGA AGAGGAAAAT GTGCCAGAGA AAGATAATGA AGAGGATGAA 
GAGAAAGAAG GCTCAGAAGC AGAGCAACCC CAGGGTGGCT CCATCATTTC AGCTGAATAC 
CTTGGGCAAA GTCTGCACCA GGAAGGCTTT TCTGCTCCCC TCCTCTGGCT CCAGAACTGC 
CTCATCCGAG CAGCAGATGA TCGGGAAGAG GCCGGCTGCT CCCAGGCAGT TCCGTTGGTG 
CCACTGACAG AAGAAAATGA AGAAGCGATG GAAAATGAAC AGTTTCAACA GCTGTTGCGA 
AAGCTAGGGG TTCGGCCTCC TGCCTCTGGA CAGGAAACCT TCTGGCGAAT TCCAGCCAAA 
CTGAATCCCA CCCAGCTCCG GAGGATGTCA GCTTCCTTGA GCCAAGCAGA AGAGGAGGAA 
AAGCTTCAGT TAGGATTAGA ATCTAAGGTC CCTGGGGAGC CAGGCCTAGA GGAGGAGCAC 
CAACAGGAAG AGGAGCATCA ACAAGAGGAG GAGCATCAAA AGGAGCACCG AGCACATGCC 
CTGAGGACCC TCATGTTAGC CCGGAAAAAG GAAGCAGGCC TGGTGTCGCC AGAGGAGGAA 
GGGACCAGTG GTGGGAAAGA GCAGCTGATA ATGGCACCCA AGAAGCGACA ACTGCTGGAC 
AGCGATGAGG AGCAGGAGGA AGATAAAGCA CCAGAGTTGG GAGTTCCAGG AATCCGGAAG 
AAGAAACGGT TTCAGATAGA GGATGATGAT GAGAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TIMELESS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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