AFF2 cDNA ORF clone, Bos taurus(cattle)

The following AFF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50517 XM_024988032.1
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa50518 XM_024988033.1
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa50519 XM_024988034.1
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa50520 XM_024988035.1
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa50520 XM_024988036.1
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa38653 NM_001191543.2
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35220 XM_005227590.4
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35221 XM_005227591.4
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35222 XM_005227592.4
Latest version!
Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa35222 XM_005227592.3 Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa38653 NM_001191543.1 Bos taurus AF4/FMR2 family member 2 (AFF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35220 XM_005227590.3 Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35221 XM_005227591.3 Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50517
    Clone ID Related Accession (Same CDS sequence) XM_024988032.1
    Accession Version XM_024988032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843800.1
    CDS276..4271
    Translation

    Target ORF information:

    RefSeq Version XM_024988032.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50518
    Clone ID Related Accession (Same CDS sequence) XM_024988033.1
    Accession Version XM_024988033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAAGTCCCGT TGAAGCCGAT GCTTCTCCTT TCCCAGGACA CCTCACCCCC
    TGTGCCCAGA TGCAAAAGCC AGAGTCGGGA GTGCTTGAGC AGCCATTAGT AAGCCTTCCC
    AGTGACCCAA GCTGTGTTGA AGAAATCTTG CGGGAGATGA CCCATCCCTG GCCTACTCCT
    CTCACTGCCA TGCATACTCC CGGAAATTCT GAGCAGAGCA CACTTTCCAT CCCAGGACAG
    GAATCCCAAC ATCTGACTCC AGGATTTGCC TTACAAAAGT GGAGTGACCC GACCAGCAGA
    GCTTCTACAA AGATGCTTGA AGATGACTTG AAGCTGAGCA GTGATGAAGA TGACCTTGAG
    CCCATGAAGA CCTTGACCAC TCAGTGCACT GCCACTGAGC TCTACCAGGC TGTTGAAAAG
    GCAAAACCTA AGAATAATCC TGTGAACCCA CTCTTGGCCA CGCCCCAACC CCCACCTGCA
    GCGCCCACCA GTGGGGGATC CGGCAGCTCC AGTGACTCAG AGAGCAGCTC AGAGTCTGAC
    TCAGACACTG AAAGCAGCAC CACTGACAGC GAAGCCAATG AGGCGCCGCG AGTGACCACT
    CCAGAGCCTG AGCCGCCCTC CACTAACAAG TGGCAGTTGG ACAAATGGCT TAACAAAGTG
    ACTTCTCAAA ACAAATCTTT TATTTGTGGC CAAAATGAAA CACCCATGGA GACGATTTCT
    GTGCCTCCTC CAGTCATTCA GCCAATGGAA GTCCAGGTCA AGGTGAAGGC AAACCCCAGC
    CAGGTCCTGC CTGAACCCAA AGAGAGGCCT CTCCTTGGTC TTATCAGGGA GAAAGCCCGT
    CCACGACCCA CCCTGAAAAC TCCAGAAACA AAGGCTCTGA AGCATAAGTT GTCCACAACT
    GTTGAGACAG CATCTCAAAG GACAATTGGG AAAAAACAGC CCAAAAGGGT CGAGAAGAAC
    ACCAGCGTTG AGGAATTTAC CTGGCCCAAA CCAAATATCA CCAGCAGCAC TCCCAAAGAA
    AAGGAAAGCG GGGAGCTTCC TGACCTCCCT AGAGGGCGCA GCAAAGCCGT TGCCCACAAG
    CCGGTTCCCA GGAAAGAACC GAGGGCCAGC ATCCCCTTGG CTCCCGAGAA GAAAAAGTAC
    CGAGGGCCTG GCAAGACGGT TCCAAAGTCC CGGGAATTCA TTGAGACCGA TTCGTCTACA
    TCTGATTCCA ACACGGATCA GGAAGAGCCC CTGCAGATCA AAGTCCTGCC TCCATGCATG
    GCTCCAGGGG GCAACACGAC CAAATCCAAG GAAGCCGGAG GGGCCAGCCT GACCCTCAGC
    AGCTTTCTCA GCAGTGGCAA TGGCAACAAC CCACCAGTCC CGAGTGAGGA ACCTACTCAG
    TCACCGGTCC CTGTCACACA AACTGAGGTT CTATCTCCTG TCCGAGATCA GGAGAATCTG
    AAAAATCTCT GGGTGAAGAT TGACCTTGAC CTACTGTCTC GAGTGCCTGG CCAGAACTCA
    CTCCAGGCAA CGTCAGCCAA GCTAGACCAC AAGGAGACAG CCTCAAAGCC CAAGCGGCAG
    ACGGCTGCGG TGCCTGCAGA GAAAGCCGCC CCCAAGGGCA AGCGTAAGCA CAAGCCAACA
    GAAGTTGCAG GGAAAATCCC TGAGAAGAAG CAGCGCCTGG AGGAGGCAGC CACCATCTGC
    CTGCTGCCAC CTTGCATCTC GCCAGCTCCC CCCCAGAAGC CTCCCAGCAC TAAAGAAAAT
    AATTCATCCA GGAGAGCAAA TAGAAGAAAA GAAGAAAAGC TGTTTCCTCC TCCACTTTCC
    CCACTGCCAG AGGACCCTCC ACGTCGCAGG AACATCAGCG GCAATAATGG TCCCTTCAGT
    CGAGACAAAA CCATTCCTTT GATTGGACAG ATCACCTCGA CCAAACCTAA GAGGAATGAA
    GGCAAATTCT GTGCTACTTT CAAAGGGATA GCTGTAAACG AGGGAGACAC TCCAAAAAAG
    GCAGCCCCTG GCACTGTCAC CAACACTGCT ATCGCCTCTG CCACTGCCAC TGCCATTGTC
    ACCAGCACTG CCACAGCCAC TGCCACGGCC ACTACCACAA CCACTACCAC AACCATTTCC
    ACCACCACCT CCGCCATCAC CACTGGCCTC ATGGATAGCA GTCACCTGGA GATGACATCG
    TGGGCGGCCC TGCCCCTTCT GTCCACCAGC ACCACCAATG TCCGGAGACC CAAGCTCACT
    TTTGATGACT CGGTTCACAA TGCTGATTAT TACATGCAAG AGGCTAAGAA GCTGAAGCAC
    AAAGCTGATG CACTGTTCGA GAAATTTGGC AAAGCCGTGA ATTACGCGGA TGCAGCCCTG
    TCCTTCACTG AATGTGGCAA CGCGATGGAG AGAGACCCTC TGGAAGCCAA GTCTCCATAC
    ACCATGTACT CCGAGACTGT GGAACTCCTC AGGTATGCCA TGAGGCTGAA GAATTTTGCA
    AGCCCCCTGG CTTCAGATGG GGACAAAAAG CTGGCTGTAC TATGCTACAG ATGCCTGTCA
    CTTCTCTACC TGAGGATGTT CAAACTGAAG AAGGACCATG CCATGAAGTA CTCCAGATCA
    CTGATGGAAT ATTTTAAGCA AAATGCTTCA AAAGTCGCTC AGATACCATC TCCATGGGTA
    GGCAATGGAA AGAATACTCC ATCCCCAGTG TCTCTCAACA ACGTCTCTCC CATCAACACA
    ATGGGGAACT GTAACAACGG CCCAGTCACC ATCCCACAGC GCATTCACCA CATGGCCGCC
    AGCCACGTCA ACATCACGAG CAATGTGTTA CGGGGCTATG AACACTGGGA CATGGCTGAC
    AAACTGACAA GAGAGAACAA AGAATTCTTT GGCGATCTGG ACACGTTGAT GGGGCCCCTG
    ACGCAGCACA GCAGCATGAC CAATCTCGTC CGCTACGTTC GCCAGGGACT GTGCTGGCTG
    CGCATCGACG CCCACTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843801.1
    CDS276..4259
    Translation

    Target ORF information:

    RefSeq Version XM_024988033.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAAGTCCCGT TGAAGCCGAT GCTTCTCCTT TCCCAGGACA CCTCACCCCC
    TGTGCCCAGA TGCAAAAGCC AGAGTCGGGA GTGCTTGAGC AGCCATTAGT AAGCCTTCCC
    AGTGACCCAA GCTGTGTTGA AGAAATCTTG CGGGAGATGA CCCATCCCTG GCCTACTCCT
    CTCACTGCCA TGCATACTCC CGGAAATTCT GAGCAGAGCA CACTTTCCAT CCCAGGACAG
    GAATCCCAAC ATCTGACTCC AGGATTTGCC TTACAAAAGT GGAGTGACCC GACCAGCAGA
    GCTTCTACAA AGATGCTTGA AGATGACTTG AAGCTGAGCA GTGATGAAGA TGACCTTGAG
    CCCATGAAGA CCTTGACCAC TCAGTGCACT GCCACTGAGC TCTACCAGGC TGTTGAAAAG
    GCAAAACCTA AGAATAATCC TGTGAACCCA CTCTTGGCCA CGCCCCAACC CCCACCTGCA
    GCGCCCACCA GTGGGGGATC CGGCAGCTCC AGTGACTCAG AGAGCAGCTC AGAGTCTGAC
    TCAGACACTG AAAGCAGCAC CACTGACAGC GAAGCCAATG AGGCGCCGCG AGTGACCACT
    CCAGAGCCTG AGCCGCCCTC CACTAACAAG TGGCAGTTGG ACAAATGGCT TAACAAAGTG
    ACTTCTCAAA ACAAATCTTT TATTTGTGGC CAAAATGAAA CACCCATGGA GACGATTTCT
    GTGCCTCCTC CAGTCATTCA GCCAATGGAA GTCCAGGTCA AGGTGAAGGC AAACCCCAGC
    CAGGTCCTGC CTGAACCCAA AGAGAGGCCT CTCCTTGGTC TTATCAGGGA GAAAGCCCGT
    CCACGACCCA CCCTGAAAAC TCCAGAAACA AAGGCTCTGA AGCATAAGTT GTCCACAACT
    GTTGAGACAG CATCTCAAAG GACAATTGGG AAAAAACAGC CCAAAAGGGT CGAGAAGAAC
    ACCAGCGTTG AGGAATTTAC CTGGCCCAAA CCAAATATCA CCAGCAGCAC TCCCAAAGAA
    AAGGAAAGCG GGGAGCTTCC TGACCTCCCT AGAGGGCGCA GCAAAGCCGT TGCCCACAAG
    CCGGTTCCCA GGAAAGAACC GAGGGCCAGC ATCCCCTTGG CTCCCGAGAA GAAAAAGTAC
    CGAGGGCCTG GCAAGACGGT TCCAAAGTCC CGGGAATTCA TTGAGACCGA TTCGTCTACA
    TCTGATTCCA ACACGGATCA GGAAGAGCCC CTGCAGATCA AAGTCCTGCC TCCATGCATG
    GCTCCAGGGG GCAACACGAC CAAATCCAAG GAAGCCGGAG GGGCCAGCCT GACCCTCAGC
    AGCTTTCTCA GCAGTGGCAA TGGCAACAAC CCACCAGTCC CGAGTGAGGA ACCTACTCAG
    TCACCGGTCC CTGTCACACA AACTGAGGTT CTATCTCCTG TCCGAGATCA GGAGAATCTG
    AAAAATCTCT GGGTGAAGAT TGACCTTGAC CTACTGTCTC GAGTGCCTGG CCAGAACTCA
    CTCCAGGCAA CGTCAGCCAA GCTAGACCAC AAGGAGACAG CCTCAAAGCC CAAGCGGCAG
    ACGGCTGCGG TGCCTGCAGA GAAAGCCGCC CCCAAGGGCA AGCGTAAGCA CAAGCCAACA
    GAAGTTGCAG GGAAAATCCC TGAGAAGAAG CAGCGCCTGG AGGAGGCAGC CACCATCTGC
    CTGCTGCCAC CTTGCATCTC GCCAGCTCCC CCCCAGAAGC CTCCCAGCAC TAAAGAAAAT
    AATTCATCCA GGAGAGCAAA TAGAAGAAAA GAAGAAAAGC TGTTTCCTCC TCCACTTTCC
    CCACTGCCAG AGGACCCTCC ACGTCGCAGG AACATCAGCG GCAATAATGG TCCCTTCAGT
    CGAGACAAAA CCATTCCTTT GATTGGACAG ATCACCTCGA CCAAACCTAA GAGGAATGAA
    GGCAAATTCT GTGCTACTTT CAAAGGGATA GCTGTAAACG AGGGAGACAC TCCAAAAAAG
    GCAGCCCCTG GCACTGTCAC CAACACTGCT ATCGCCTCTG CCACTGCCAC TGCCATTGTC
    ACCAGCACTG CCACAGCCAC TGCCACGGCC ACTACCACAA CCACTACCAC AACCATTTCC
    ACCACCACCT CCGCCATCAC CACTGGCCTC ATGGATAGCA GTCACCTGGA GATGACATCG
    TGGGCGGCCC TGCCCCTTCT GTCCACCAGC ACCACCAATG TCCGGAGACC CAAGCTCACT
    TTTGATGACT CGGTTCACAA TGCTGATTAT TACATGCAAG AGGCTAAGAA GCTGAAGCAC
    AAAGCTGATG CACTGTTCGA GAAATTTGGC AAAGCCGTGA ATTACGCGGA TGCAGCCCTG
    TCCTTCACTG AATGTGGCAA CGCGATGGAG AGAGACCCTC TGGAAGCCAA GTCTCCATAC
    ACCATGTACT CCGAGACTGT GGAACTCCTC AGGTATGCCA TGAGGCTGAA GAATTTTGCA
    AGCCCCCTGG CTTCAGATGG GGACAAAAAG CTGGCTGTAC TATGCTACAG ATGCCTGTCA
    CTTCTCTACC TGAGGATGTT CAAACTGAAG AAGGACCATG CCATGAAGTA CTCCAGATCA
    CTGATGGAAT ATTTTAAGCA AAATGCTTCA AAAGTCGCTC AGATACCATC TCCATGGGTA
    GGCAATGGAA AGAATACTCC ATCCCCAGTG TCTCTCAACA ACGTCTCTCC CATCAACACA
    ATGGGGAACT GTAACAACGG CCCAGTCACC ATCCCACAGC GCATTCACCA CATGGCCGCC
    AGCCACGTCA ACATCACGAG CAATGTGTTA CGGGGCTATG AACACTGGGA CATGGCTGAC
    AAACTGACAA GAGAGAACAA AGAATTCTTT GGCGATCTGG ACACGTTGAT GGGGCCCCTG
    ACGCAGCACA GCAGCATGAC CAATCTCGTC CGCTACGTTC GCCAGGGACT GTGCTGGCTG
    CGCATCGACG CCCACTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50519
    Clone ID Related Accession (Same CDS sequence) XM_024988034.1
    Accession Version XM_024988034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAATC CCAACATCTG
    ACTCCAGGAT TTGCCTTACA AAAGTGGAGT GACCCGACCA GCAGAGCTTC TACAAAGATG
    CTTGAAGATG ACTTGAAGCT GAGCAGTGAT GAAGATGACC TTGAGCCCAT GAAGACCTTG
    ACCACTCAGT GCACTGCCAC TGAGCTCTAC CAGGCTGTTG AAAAGGCAAA ACCTAAGAAT
    AATCCTGTGA ACCCACTCTT GGCCACGCCC CAACCCCCAC CTGCAGCGCC CACCAGTGGG
    GGATCCGGCA GCTCCAGTGA CTCAGAGAGC AGCTCAGAGT CTGACTCAGA CACTGAAAGC
    AGCACCACTG ACAGCGAAGC CAATGAGGCG CCGCGAGTGA CCACTCCAGA GCCTGAGCCG
    CCCTCCACTA ACAAGTGGCA GTTGGACAAA TGGCTTAACA AAGTGACTTC TCAAAACAAA
    TCTTTTATTT GTGGCCAAAA TGAAACACCC ATGGAGACGA TTTCTGTGCC TCCTCCAGTC
    ATTCAGCCAA TGGAAGTCCA GGTCAAGGTG AAGGCAAACC CCAGCCAGGT CCTGCCTGAA
    CCCAAAGAGA GGCCTCTCCT TGGTCTTATC AGGGAGAAAG CCCGTCCACG ACCCACCCTG
    AAAACTCCAG AAACAAAGGC TCTGAAGCAT AAGTTGTCCA CAACTGTTGA GACAGCATCT
    CAAAGGACAA TTGGGAAAAA ACAGCCCAAA AGGGTCGAGA AGAACACCAG CGTTGAGGAA
    TTTACCTGGC CCAAACCAAA TATCACCAGC AGCACTCCCA AAGAAAAGGA AAGCGGGGAG
    CTTCCTGACC TCCCTAGAGG GCGCAGCAAA GCCGTTGCCC ACAAGCCGGT TCCCAGGAAA
    GAACCGAGGG CCAGCATCCC CTTGGCTCCC GAGAAGAAAA AGTACCGAGG GCCTGGCAAG
    ACGGTTCCAA AGTCCCGGGA ATTCATTGAG ACCGATTCGT CTACATCTGA TTCCAACACG
    GATCAGGAAG AGCCCCTGCA GATCAAAGTC CTGCCTCCAT GCATGGCTCC AGGGGGCAAC
    ACGACCAAAT CCAAGGAAGC CGGAGGGGCC AGCCTGACCC TCAGCAGCTT TCTCAGCAGT
    GGCAATGGCA ACAACCCACC AGTCCCGAGT GAGGAACCTA CTCAGTCACC GGTCCCTGTC
    ACACAAACTG AGGTTCTATC TCCTGTCCGA GATCAGGAGA ATCTGAAAAA TCTCTGGGTG
    AAGATTGACC TTGACCTACT GTCTCGAGTG CCTGGCCAGA ACTCACTCCA GGCAACGTCA
    GCCAAGCTAG ACCACAAGGA GACAGCCTCA AAGCCCAAGC GGCAGACGGC TGCGGTGCCT
    GCAGAGAAAG CCGCCCCCAA GGGCAAGCGT AAGCACAAGC CAACAGAAGT TGCAGGGAAA
    ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG GCAGCCACCA TCTGCCTGCT GCCACCTTGC
    ATCTCGCCAG CTCCCCCCCA GAAGCCTCCC AGCACTAAAG AAAATAATTC ATCCAGGAGA
    GCAAATAGAA GAAAAGAAGA AAAGCTGTTT CCTCCTCCAC TTTCCCCACT GCCAGAGGAC
    CCTCCACGTC GCAGGAACAT CAGCGGCAAT AATGGTCCCT TCAGTCGAGA CAAAACCATT
    CCTTTGATTG GACAGATCAC CTCGACCAAA CCTAAGAGGA ATGAAGGCAA ATTCTGTGCT
    ACTTTCAAAG GGATAGCTGT AAACGAGGGA GACACTCCAA AAAAGGCAGC CCCTGGCACT
    GTCACCAACA CTGCTATCGC CTCTGCCACT GCCACTGCCA TTGTCACCAG CACTGCCACA
    GCCACTGCCA CGGCCACTAC CACAACCACT ACCACAACCA TTTCCACCAC CACCTCCGCC
    ATCACCACTG GCCTCATGGA TAGCAGTCAC CTGGAGATGA CATCGTGGGC GGCCCTGCCC
    CTTCTGTCCA CCAGCACCAC CAATGTCCGG AGACCCAAGC TCACTTTTGA TGACTCGGTT
    CACAATGCTG ATTATTACAT GCAAGAGGCT AAGAAGCTGA AGCACAAAGC TGATGCACTG
    TTCGAGAAAT TTGGCAAAGC CGTGAATTAC GCGGATGCAG CCCTGTCCTT CACTGAATGT
    GGCAACGCGA TGGAGAGAGA CCCTCTGGAA GCCAAGTCTC CATACACCAT GTACTCCGAG
    ACTGTGGAAC TCCTCAGGTA TGCCATGAGG CTGAAGAATT TTGCAAGCCC CCTGGCTTCA
    GATGGGGACA AAAAGCTGGC TGTACTATGC TACAGATGCC TGTCACTTCT CTACCTGAGG
    ATGTTCAAAC TGAAGAAGGA CCATGCCATG AAGTACTCCA GATCACTGAT GGAATATTTT
    AAGCAAAATG CTTCAAAAGT CGCTCAGATA CCATCTCCAT GGGTAGGCAA TGGAAAGAAT
    ACTCCATCCC CAGTGTCTCT CAACAACGTC TCTCCCATCA ACACAATGGG GAACTGTAAC
    AACGGCCCAG TCACCATCCC ACAGCGCATT CACCACATGG CCGCCAGCCA CGTCAACATC
    ACGAGCAATG TGTTACGGGG CTATGAACAC TGGGACATGG CTGACAAACT GACAAGAGAG
    AACAAAGAAT TCTTTGGCGA TCTGGACACG TTGATGGGGC CCCTGACGCA GCACAGCAGC
    ATGACCAATC TCGTCCGCTA CGTTCGCCAG GGACTGTGCT GGCTGCGCAT CGACGCCCAC
    TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843802.1
    CDS276..4184
    Translation

    Target ORF information:

    RefSeq Version XM_024988034.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAATC CCAACATCTG
    ACTCCAGGAT TTGCCTTACA AAAGTGGAGT GACCCGACCA GCAGAGCTTC TACAAAGATG
    CTTGAAGATG ACTTGAAGCT GAGCAGTGAT GAAGATGACC TTGAGCCCAT GAAGACCTTG
    ACCACTCAGT GCACTGCCAC TGAGCTCTAC CAGGCTGTTG AAAAGGCAAA ACCTAAGAAT
    AATCCTGTGA ACCCACTCTT GGCCACGCCC CAACCCCCAC CTGCAGCGCC CACCAGTGGG
    GGATCCGGCA GCTCCAGTGA CTCAGAGAGC AGCTCAGAGT CTGACTCAGA CACTGAAAGC
    AGCACCACTG ACAGCGAAGC CAATGAGGCG CCGCGAGTGA CCACTCCAGA GCCTGAGCCG
    CCCTCCACTA ACAAGTGGCA GTTGGACAAA TGGCTTAACA AAGTGACTTC TCAAAACAAA
    TCTTTTATTT GTGGCCAAAA TGAAACACCC ATGGAGACGA TTTCTGTGCC TCCTCCAGTC
    ATTCAGCCAA TGGAAGTCCA GGTCAAGGTG AAGGCAAACC CCAGCCAGGT CCTGCCTGAA
    CCCAAAGAGA GGCCTCTCCT TGGTCTTATC AGGGAGAAAG CCCGTCCACG ACCCACCCTG
    AAAACTCCAG AAACAAAGGC TCTGAAGCAT AAGTTGTCCA CAACTGTTGA GACAGCATCT
    CAAAGGACAA TTGGGAAAAA ACAGCCCAAA AGGGTCGAGA AGAACACCAG CGTTGAGGAA
    TTTACCTGGC CCAAACCAAA TATCACCAGC AGCACTCCCA AAGAAAAGGA AAGCGGGGAG
    CTTCCTGACC TCCCTAGAGG GCGCAGCAAA GCCGTTGCCC ACAAGCCGGT TCCCAGGAAA
    GAACCGAGGG CCAGCATCCC CTTGGCTCCC GAGAAGAAAA AGTACCGAGG GCCTGGCAAG
    ACGGTTCCAA AGTCCCGGGA ATTCATTGAG ACCGATTCGT CTACATCTGA TTCCAACACG
    GATCAGGAAG AGCCCCTGCA GATCAAAGTC CTGCCTCCAT GCATGGCTCC AGGGGGCAAC
    ACGACCAAAT CCAAGGAAGC CGGAGGGGCC AGCCTGACCC TCAGCAGCTT TCTCAGCAGT
    GGCAATGGCA ACAACCCACC AGTCCCGAGT GAGGAACCTA CTCAGTCACC GGTCCCTGTC
    ACACAAACTG AGGTTCTATC TCCTGTCCGA GATCAGGAGA ATCTGAAAAA TCTCTGGGTG
    AAGATTGACC TTGACCTACT GTCTCGAGTG CCTGGCCAGA ACTCACTCCA GGCAACGTCA
    GCCAAGCTAG ACCACAAGGA GACAGCCTCA AAGCCCAAGC GGCAGACGGC TGCGGTGCCT
    GCAGAGAAAG CCGCCCCCAA GGGCAAGCGT AAGCACAAGC CAACAGAAGT TGCAGGGAAA
    ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG GCAGCCACCA TCTGCCTGCT GCCACCTTGC
    ATCTCGCCAG CTCCCCCCCA GAAGCCTCCC AGCACTAAAG AAAATAATTC ATCCAGGAGA
    GCAAATAGAA GAAAAGAAGA AAAGCTGTTT CCTCCTCCAC TTTCCCCACT GCCAGAGGAC
    CCTCCACGTC GCAGGAACAT CAGCGGCAAT AATGGTCCCT TCAGTCGAGA CAAAACCATT
    CCTTTGATTG GACAGATCAC CTCGACCAAA CCTAAGAGGA ATGAAGGCAA ATTCTGTGCT
    ACTTTCAAAG GGATAGCTGT AAACGAGGGA GACACTCCAA AAAAGGCAGC CCCTGGCACT
    GTCACCAACA CTGCTATCGC CTCTGCCACT GCCACTGCCA TTGTCACCAG CACTGCCACA
    GCCACTGCCA CGGCCACTAC CACAACCACT ACCACAACCA TTTCCACCAC CACCTCCGCC
    ATCACCACTG GCCTCATGGA TAGCAGTCAC CTGGAGATGA CATCGTGGGC GGCCCTGCCC
    CTTCTGTCCA CCAGCACCAC CAATGTCCGG AGACCCAAGC TCACTTTTGA TGACTCGGTT
    CACAATGCTG ATTATTACAT GCAAGAGGCT AAGAAGCTGA AGCACAAAGC TGATGCACTG
    TTCGAGAAAT TTGGCAAAGC CGTGAATTAC GCGGATGCAG CCCTGTCCTT CACTGAATGT
    GGCAACGCGA TGGAGAGAGA CCCTCTGGAA GCCAAGTCTC CATACACCAT GTACTCCGAG
    ACTGTGGAAC TCCTCAGGTA TGCCATGAGG CTGAAGAATT TTGCAAGCCC CCTGGCTTCA
    GATGGGGACA AAAAGCTGGC TGTACTATGC TACAGATGCC TGTCACTTCT CTACCTGAGG
    ATGTTCAAAC TGAAGAAGGA CCATGCCATG AAGTACTCCA GATCACTGAT GGAATATTTT
    AAGCAAAATG CTTCAAAAGT CGCTCAGATA CCATCTCCAT GGGTAGGCAA TGGAAAGAAT
    ACTCCATCCC CAGTGTCTCT CAACAACGTC TCTCCCATCA ACACAATGGG GAACTGTAAC
    AACGGCCCAG TCACCATCCC ACAGCGCATT CACCACATGG CCGCCAGCCA CGTCAACATC
    ACGAGCAATG TGTTACGGGG CTATGAACAC TGGGACATGG CTGACAAACT GACAAGAGAG
    AACAAAGAAT TCTTTGGCGA TCTGGACACG TTGATGGGGC CCCTGACGCA GCACAGCAGC
    ATGACCAATC TCGTCCGCTA CGTTCGCCAG GGACTGTGCT GGCTGCGCAT CGACGCCCAC
    TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50520
    Clone ID Related Accession (Same CDS sequence) XM_024988035.1 , XM_024988036.1
    Accession Version XM_024988035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT 
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843803.1
    CDS1731..5486
    Translation

    Target ORF information:

    RefSeq Version XM_024988035.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT 
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50520
    Clone ID Related Accession (Same CDS sequence) XM_024988035.1 , XM_024988036.1
    Accession Version XM_024988036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT 
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843804.1
    CDS1877..5632
    Translation

    Target ORF information:

    RefSeq Version XM_024988036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT 
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAAGTCCC GTTGAAGCCG ATGCTTCTCC TTTCCCAGGA
    CACCTCACCC CCTGTGCCCA GATGCAAAAG CCAGAGTCGG GAGTGCTTGA GCAGCCATTA
    GTAAGCCTTC CCAGTGACCC AAGCTGTGTT GAAGAAATCT TGCGGGAGAT GACCCATCCC
    TGGCCTACTC CTCTCACTGC CATGCATACT CCCGGAAATT CTGAGCAGAG CACACTTTCC
    ATCCCAGGAC AGGAATCCCA ACATCTGACT CCAGGATTTG CCTTACAAAA GTGGAGTGAC
    CCGACCAGCA GAGCTTCTAC AAAGATGCTT GAAGATGACT TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCCATGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAAGAATAAT CCTGTGAACC CACTCTTGGC CACGCCCCAA
    CCCCCACCTG CAGCGCCCAC CAGTGGGGGA TCCGGCAGCT CCAGTGACTC AGAGAGCAGC
    TCAGAGTCTG ACTCAGACAC TGAAAGCAGC ACCACTGACA GCGAAGCCAA TGAGGCGCCG
    CGAGTGACCA CTCCAGAGCC TGAGCCGCCC TCCACTAACA AGTGGCAGTT GGACAAATGG
    CTTAACAAAG TGACTTCTCA AAACAAATCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACGATTT CTGTGCCTCC TCCAGTCATT CAGCCAATGG AAGTCCAGGT CAAGGTGAAG
    GCAAACCCCA GCCAGGTCCT GCCTGAACCC AAAGAGAGGC CTCTCCTTGG TCTTATCAGG
    GAGAAAGCCC GTCCACGACC CACCCTGAAA ACTCCAGAAA CAAAGGCTCT GAAGCATAAG
    TTGTCCACAA CTGTTGAGAC AGCATCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAGG
    GTCGAGAAGA ACACCAGCGT TGAGGAATTT ACCTGGCCCA AACCAAATAT CACCAGCAGC
    ACTCCCAAAG AAAAGGAAAG CGGGGAGCTT CCTGACCTCC CTAGAGGGCG CAGCAAAGCC
    GTTGCCCACA AGCCGGTTCC CAGGAAAGAA CCGAGGGCCA GCATCCCCTT GGCTCCCGAG
    AAGAAAAAGT ACCGAGGGCC TGGCAAGACG GTTCCAAAGT CCCGGGAATT CATTGAGACC
    GATTCGTCTA CATCTGATTC CAACACGGAT CAGGAAGAGC CCCTGCAGAT CAAAGTCCTG
    CCTCCATGCA TGGCTCCAGG GGGCAACACG ACCAAATCCA AGGAAGCCGG AGGGGCCAGC
    CTGACCCTCA GCAGCTTTCT CAGCAGTGGC AATGGCAACA ACCCACCAGT CCCGAGTGAG
    GAACCTACTC AGTCACCGGT CCCTGTCACA CAAACTGAGG TTCTATCTCC TGTCCGAGAT
    CAGGAGAATC TGAAAAATCT CTGGGTGAAG ATTGACCTTG ACCTACTGTC TCGAGTGCCT
    GGCCAGAACT CACTCCAGGC AACGTCAGCC AAGCTAGACC ACAAGGAGAC AGCCTCAAAG
    CCCAAGCGGC AGACGGCTGC GGTGCCTGCA GAGAAAGCCG CCCCCAAGGG CAAGCGTAAG
    CACAAGCCAA CAGAAGTTGC AGGGAAAATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCA
    GCCACCATCT GCCTGCTGCC ACCTTGCATC TCGCCAGCTC CCCCCCAGAA GCCTCCCAGC
    ACTAAAGAAA ATAATTCATC CAGGAGAGCA AATAGAAGAA AAGAAGAAAA GCTGTTTCCT
    CCTCCACTTT CCCCACTGCC AGAGGACCCT CCACGTCGCA GGAACATCAG CGGCAATAAT
    GGTCCCTTCA GTCGAGACAA AACCATTCCT TTGATTGGAC AGATCACCTC GACCAAACCT
    AAGAGGAATG AAGGCAAATT CTGTGCTACT TTCAAAGGGA TAGCTGTAAA CGAGGGAGAC
    ACTCCAAAAA AGGCAGCCCC TGGCACTGTC ACCAACACTG CTATCGCCTC TGCCACTGCC
    ACTGCCATTG TCACCAGCAC TGCCACAGCC ACTGCCACGG CCACTACCAC AACCACTACC
    ACAACCATTT CCACCACCAC CTCCGCCATC ACCACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACAT CGTGGGCGGC CCTGCCCCTT CTGTCCACCA GCACCACCAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAGGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCCGT GAATTACGCG
    GATGCAGCCC TGTCCTTCAC TGAATGTGGC AACGCGATGG AGAGAGACCC TCTGGAAGCC
    AAGTCTCCAT ACACCATGTA CTCCGAGACT GTGGAACTCC TCAGGTATGC CATGAGGCTG
    AAGAATTTTG CAAGCCCCCT GGCTTCAGAT GGGGACAAAA AGCTGGCTGT ACTATGCTAC
    AGATGCCTGT CACTTCTCTA CCTGAGGATG TTCAAACTGA AGAAGGACCA TGCCATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC TCAGATACCA
    TCTCCATGGG TAGGCAATGG AAAGAATACT CCATCCCCAG TGTCTCTCAA CAACGTCTCT
    CCCATCAACA CAATGGGGAA CTGTAACAAC GGCCCAGTCA CCATCCCACA GCGCATTCAC
    CACATGGCCG CCAGCCACGT CAACATCACG AGCAATGTGT TACGGGGCTA TGAACACTGG
    GACATGGCTG ACAAACTGAC AAGAGAGAAC AAAGAATTCT TTGGCGATCT GGACACGTTG
    ATGGGGCCCC TGACGCAGCA CAGCAGCATG ACCAATCTCG TCCGCTACGT TCGCCAGGGA
    CTGTGCTGGC TGCGCATCGA CGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38653
    Clone ID Related Accession (Same CDS sequence) NM_001191543.1 , NM_001191543.2
    Accession Version NM_001191543.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02068037.1, DAAA02068039.1, DAAA02068043.1, DAAA02068044.1, DAAA02068045.1, DAAA02068056.1 and DAAA02068057.1. On Apr 20, 2011 this sequence version replaced XM_002707156.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822100 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AATCCCAACA TCTGACTCCA GGATTTGCCT TACAAAAGTG GAGTGACCCG
    ACCAGCAGAG CTTCTACAAA GATGCTTGAA GATGACTTGA AGCTGAGCAG TGATGAAGAT
    GACCTTGAGC CCATGAAGAC CTTGACCACT CAGTGCACTG CCACTGAGCT CTACCAGGCT
    GTTGAAAAGG CAAAACCTAA GAATAATCCT GTGAACCCAC TCTTGGCCAC GCCCCAACCC
    CCACCTGCAG CGCCCACCAG TGGGGGATCC GGCAGCTCCA GTGACTCAGA GAGCAGCTCA
    GAGTCTGACT CAGACACTGA AAGCAGCACC ACTGACAGCG AAGCCAATGA GGCGCCGCGA
    GTGACCACTC CAGAGCCTGA GCCGCCCTCC ACTAACAAGT GGCAGTTGGA CAAATGGCTT
    AACAAAGTGA CTTCTCAAAA CAAATCTTTT ATTTGTGGCC AAAATGAAAC ACCCATGGAG
    ACGATTTCTG TGCCTCCTCC AGTCATTCAG CCAATGGAAG TCCAGGTCAA GGTGAAGGCA
    AACCCCAGCC AGGTCCTGCC TGAACCCAAA GAGAGGCCTC TCCTTGGTCT TATCAGGGAG
    AAAGCCCGTC CACGACCCAC CCTGAAAACT CCAGAAACAA AGGCTCTGAA GCATAAGTTG
    TCCACAACTG TTGAGACAGC ATCTCAAAGG ACAATTGGGA AAAAACAGCC CAAAAGGGTC
    GAGAAGAACA CCAGCGTTGA GGAATTTACC TGGCCCAAAC CAAATATCAC CAGCAGCACT
    CCCAAAGAAA AGGAAAGCGG GGAGCTTCCT GACCTCCCTA GAGGGCGCAG CAAAGCCGTT
    GCCCACAAGC CGGTTCCCAG GAAAGAACCG AGGGCCAGCA TCCCCTTGGC TCCCGAGAAG
    AAAAAGTACC GAGGGCCTGG CAAGACGGTT CCAAAGTCCC GGGAATTCAT TGAGACCGAT
    TCGTCTACAT CTGATTCCAA CACGGATCAG GAAGAGCCCC TGCAGATCAA AGTCCTGCCT
    CCATGCATGG CTCCAGGGGG CAACACGACC AAATCCAAGG AAGCCGGAGG GGCCAGCCTG
    ACCCTCAGCA GCTTTCTCAG CAGTGGCAAT GGCAACAACC CACCAGTCCC GAGTGAGGAA
    CCTACTCAGT CACCGGTCCC TGTCACACAA ACTGAGGTTC TATCTCCTGT CCGAGATCAG
    GAGAATCTGA AAAATCTCTG GGTGAAGATT GACCTTGACC TACTGTCTCG AGTGCCTGGC
    CAGAACTCAC TCCAGGCAAC GTCAGCCAAG CTAGACCACA AGGAGACAGC CTCAAAGCCC
    AAGCGGCAGA CGGCTGCGGT GCCTGCAGAG AAAGCCGCCC CCAAGGGCAA GCGTAAGCAC
    AAGCCAACAG AAGTTGCAGG GAAAATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCAGCC
    ACCATCTGCC TGCTGCCACC TTGCATCTCG CCAGCTCCCC CCCAGAAGCC TCCCAGCACT
    AAAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAAG AAGAAAAGCT GTTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGTCGCAGGA ACATCAGCGG CAATAATGGT
    CCCTTCAGTC GAGACAAAAC CATTCCTTTG ATTGGACAGA TCACCTCGAC CAAACCTAAG
    AGGAATGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAG CTGTAAACGA GGGAGACACT
    CCAAAAAAGG CAGCCCCTGG CACTGTCACC AACACTGCTA TCGCCTCTGC CACTGCCACT
    GCCATTGTCA CCAGCACTGC CACAGCCACT GCCACGGCCA CTACCACAAC CACTACCACA
    ACCATTTCCA CCACCACCTC CGCCATCACC ACTGGCCTCA TGGATAGCAG TCACCTGGAG
    ATGACATCGT GGGCGGCCCT GCCCCTTCTG TCCACCAGCA CCACCAATGT CCGGAGACCC
    AAGCTCACTT TTGATGACTC GGTTCACAAT GCTGATTATT ACATGCAAGA GGCTAAGAAG
    CTGAAGCACA AAGCTGATGC ACTGTTCGAG AAATTTGGCA AAGCCGTGAA TTACGCGGAT
    GCAGCCCTGT CCTTCACTGA ATGTGGCAAC GCGATGGAGA GAGACCCTCT GGAAGCCAAG
    TCTCCATACA CCATGTACTC CGAGACTGTG GAACTCCTCA GGTATGCCAT GAGGCTGAAG
    AATTTTGCAA GCCCCCTGGC TTCAGATGGG GACAAAAAGC TGGCTGTACT ATGCTACAGA
    TGCCTGTCAC TTCTCTACCT GAGGATGTTC AAACTGAAGA AGGACCATGC CATGAAGTAC
    TCCAGATCAC TGATGGAATA TTTTAAGCAA AATGCTTCAA AAGTCGCTCA GATACCATCT
    CCATGGGTAG GCAATGGAAA GAATACTCCA TCCCCAGTGT CTCTCAACAA CGTCTCTCCC
    ATCAACACAA TGGGGAACTG TAACAACGGC CCAGTCACCA TCCCACAGCG CATTCACCAC
    ATGGCCGCCA GCCACGTCAA CATCACGAGC AATGTGTTAC GGGGCTATGA ACACTGGGAC
    ATGGCTGACA AACTGACAAG AGAGAACAAA GAATTCTTTG GCGATCTGGA CACGTTGATG
    GGGCCCCTGA CGCAGCACAG CAGCATGACC AATCTCGTCC GCTACGTTCG CCAGGGACTG
    TGCTGGCTGC GCATCGACGC CCACTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178472.1
    CDS1..3813
    Translation

    Target ORF information:

    RefSeq Version NM_001191543.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AATCCCAACA TCTGACTCCA GGATTTGCCT TACAAAAGTG GAGTGACCCG
    ACCAGCAGAG CTTCTACAAA GATGCTTGAA GATGACTTGA AGCTGAGCAG TGATGAAGAT
    GACCTTGAGC CCATGAAGAC CTTGACCACT CAGTGCACTG CCACTGAGCT CTACCAGGCT
    GTTGAAAAGG CAAAACCTAA GAATAATCCT GTGAACCCAC TCTTGGCCAC GCCCCAACCC
    CCACCTGCAG CGCCCACCAG TGGGGGATCC GGCAGCTCCA GTGACTCAGA GAGCAGCTCA
    GAGTCTGACT CAGACACTGA AAGCAGCACC ACTGACAGCG AAGCCAATGA GGCGCCGCGA
    GTGACCACTC CAGAGCCTGA GCCGCCCTCC ACTAACAAGT GGCAGTTGGA CAAATGGCTT
    AACAAAGTGA CTTCTCAAAA CAAATCTTTT ATTTGTGGCC AAAATGAAAC ACCCATGGAG
    ACGATTTCTG TGCCTCCTCC AGTCATTCAG CCAATGGAAG TCCAGGTCAA GGTGAAGGCA
    AACCCCAGCC AGGTCCTGCC TGAACCCAAA GAGAGGCCTC TCCTTGGTCT TATCAGGGAG
    AAAGCCCGTC CACGACCCAC CCTGAAAACT CCAGAAACAA AGGCTCTGAA GCATAAGTTG
    TCCACAACTG TTGAGACAGC ATCTCAAAGG ACAATTGGGA AAAAACAGCC CAAAAGGGTC
    GAGAAGAACA CCAGCGTTGA GGAATTTACC TGGCCCAAAC CAAATATCAC CAGCAGCACT
    CCCAAAGAAA AGGAAAGCGG GGAGCTTCCT GACCTCCCTA GAGGGCGCAG CAAAGCCGTT
    GCCCACAAGC CGGTTCCCAG GAAAGAACCG AGGGCCAGCA TCCCCTTGGC TCCCGAGAAG
    AAAAAGTACC GAGGGCCTGG CAAGACGGTT CCAAAGTCCC GGGAATTCAT TGAGACCGAT
    TCGTCTACAT CTGATTCCAA CACGGATCAG GAAGAGCCCC TGCAGATCAA AGTCCTGCCT
    CCATGCATGG CTCCAGGGGG CAACACGACC AAATCCAAGG AAGCCGGAGG GGCCAGCCTG
    ACCCTCAGCA GCTTTCTCAG CAGTGGCAAT GGCAACAACC CACCAGTCCC GAGTGAGGAA
    CCTACTCAGT CACCGGTCCC TGTCACACAA ACTGAGGTTC TATCTCCTGT CCGAGATCAG
    GAGAATCTGA AAAATCTCTG GGTGAAGATT GACCTTGACC TACTGTCTCG AGTGCCTGGC
    CAGAACTCAC TCCAGGCAAC GTCAGCCAAG CTAGACCACA AGGAGACAGC CTCAAAGCCC
    AAGCGGCAGA CGGCTGCGGT GCCTGCAGAG AAAGCCGCCC CCAAGGGCAA GCGTAAGCAC
    AAGCCAACAG AAGTTGCAGG GAAAATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCAGCC
    ACCATCTGCC TGCTGCCACC TTGCATCTCG CCAGCTCCCC CCCAGAAGCC TCCCAGCACT
    AAAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAAG AAGAAAAGCT GTTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGTCGCAGGA ACATCAGCGG CAATAATGGT
    CCCTTCAGTC GAGACAAAAC CATTCCTTTG ATTGGACAGA TCACCTCGAC CAAACCTAAG
    AGGAATGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAG CTGTAAACGA GGGAGACACT
    CCAAAAAAGG CAGCCCCTGG CACTGTCACC AACACTGCTA TCGCCTCTGC CACTGCCACT
    GCCATTGTCA CCAGCACTGC CACAGCCACT GCCACGGCCA CTACCACAAC CACTACCACA
    ACCATTTCCA CCACCACCTC CGCCATCACC ACTGGCCTCA TGGATAGCAG TCACCTGGAG
    ATGACATCGT GGGCGGCCCT GCCCCTTCTG TCCACCAGCA CCACCAATGT CCGGAGACCC
    AAGCTCACTT TTGATGACTC GGTTCACAAT GCTGATTATT ACATGCAAGA GGCTAAGAAG
    CTGAAGCACA AAGCTGATGC ACTGTTCGAG AAATTTGGCA AAGCCGTGAA TTACGCGGAT
    GCAGCCCTGT CCTTCACTGA ATGTGGCAAC GCGATGGAGA GAGACCCTCT GGAAGCCAAG
    TCTCCATACA CCATGTACTC CGAGACTGTG GAACTCCTCA GGTATGCCAT GAGGCTGAAG
    AATTTTGCAA GCCCCCTGGC TTCAGATGGG GACAAAAAGC TGGCTGTACT ATGCTACAGA
    TGCCTGTCAC TTCTCTACCT GAGGATGTTC AAACTGAAGA AGGACCATGC CATGAAGTAC
    TCCAGATCAC TGATGGAATA TTTTAAGCAA AATGCTTCAA AAGTCGCTCA GATACCATCT
    CCATGGGTAG GCAATGGAAA GAATACTCCA TCCCCAGTGT CTCTCAACAA CGTCTCTCCC
    ATCAACACAA TGGGGAACTG TAACAACGGC CCAGTCACCA TCCCACAGCG CATTCACCAC
    ATGGCCGCCA GCCACGTCAA CATCACGAGC AATGTGTTAC GGGGCTATGA ACACTGGGAC
    ATGGCTGACA AACTGACAAG AGAGAACAAA GAATTCTTTG GCGATCTGGA CACGTTGATG
    GGGCCCCTGA CGCAGCACAG CAGCATGACC AATCTCGTCC GCTACGTTCG CCAGGGACTG
    TGCTGGCTGC GCATCGACGC CCACTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38653
    Clone ID Related Accession (Same CDS sequence) NM_001191543.1 , NM_001191543.2
    Accession Version NM_001191543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000030.1. On Dec 20, 2018 this sequence version replaced NM_001191543.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145439 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AATCCCAACA TCTGACTCCA GGATTTGCCT TACAAAAGTG GAGTGACCCG
    ACCAGCAGAG CTTCTACAAA GATGCTTGAA GATGACTTGA AGCTGAGCAG TGATGAAGAT
    GACCTTGAGC CCATGAAGAC CTTGACCACT CAGTGCACTG CCACTGAGCT CTACCAGGCT
    GTTGAAAAGG CAAAACCTAA GAATAATCCT GTGAACCCAC TCTTGGCCAC GCCCCAACCC
    CCACCTGCAG CGCCCACCAG TGGGGGATCC GGCAGCTCCA GTGACTCAGA GAGCAGCTCA
    GAGTCTGACT CAGACACTGA AAGCAGCACC ACTGACAGCG AAGCCAATGA GGCGCCGCGA
    GTGACCACTC CAGAGCCTGA GCCGCCCTCC ACTAACAAGT GGCAGTTGGA CAAATGGCTT
    AACAAAGTGA CTTCTCAAAA CAAATCTTTT ATTTGTGGCC AAAATGAAAC ACCCATGGAG
    ACGATTTCTG TGCCTCCTCC AGTCATTCAG CCAATGGAAG TCCAGGTCAA GGTGAAGGCA
    AACCCCAGCC AGGTCCTGCC TGAACCCAAA GAGAGGCCTC TCCTTGGTCT TATCAGGGAG
    AAAGCCCGTC CACGACCCAC CCTGAAAACT CCAGAAACAA AGGCTCTGAA GCATAAGTTG
    TCCACAACTG TTGAGACAGC ATCTCAAAGG ACAATTGGGA AAAAACAGCC CAAAAGGGTC
    GAGAAGAACA CCAGCGTTGA GGAATTTACC TGGCCCAAAC CAAATATCAC CAGCAGCACT
    CCCAAAGAAA AGGAAAGCGG GGAGCTTCCT GACCTCCCTA GAGGGCGCAG CAAAGCCGTT
    GCCCACAAGC CGGTTCCCAG GAAAGAACCG AGGGCCAGCA TCCCCTTGGC TCCCGAGAAG
    AAAAAGTACC GAGGGCCTGG CAAGACGGTT CCAAAGTCCC GGGAATTCAT TGAGACCGAT
    TCGTCTACAT CTGATTCCAA CACGGATCAG GAAGAGCCCC TGCAGATCAA AGTCCTGCCT
    CCATGCATGG CTCCAGGGGG CAACACGACC AAATCCAAGG AAGCCGGAGG GGCCAGCCTG
    ACCCTCAGCA GCTTTCTCAG CAGTGGCAAT GGCAACAACC CACCAGTCCC GAGTGAGGAA
    CCTACTCAGT CACCGGTCCC TGTCACACAA ACTGAGGTTC TATCTCCTGT CCGAGATCAG
    GAGAATCTGA AAAATCTCTG GGTGAAGATT GACCTTGACC TACTGTCTCG AGTGCCTGGC
    CAGAACTCAC TCCAGGCAAC GTCAGCCAAG CTAGACCACA AGGAGACAGC CTCAAAGCCC
    AAGCGGCAGA CGGCTGCGGT GCCTGCAGAG AAAGCCGCCC CCAAGGGCAA GCGTAAGCAC
    AAGCCAACAG AAGTTGCAGG GAAAATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCAGCC
    ACCATCTGCC TGCTGCCACC TTGCATCTCG CCAGCTCCCC CCCAGAAGCC TCCCAGCACT
    AAAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAAG AAGAAAAGCT GTTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGTCGCAGGA ACATCAGCGG CAATAATGGT
    CCCTTCAGTC GAGACAAAAC CATTCCTTTG ATTGGACAGA TCACCTCGAC CAAACCTAAG
    AGGAATGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAG CTGTAAACGA GGGAGACACT
    CCAAAAAAGG CAGCCCCTGG CACTGTCACC AACACTGCTA TCGCCTCTGC CACTGCCACT
    GCCATTGTCA CCAGCACTGC CACAGCCACT GCCACGGCCA CTACCACAAC CACTACCACA
    ACCATTTCCA CCACCACCTC CGCCATCACC ACTGGCCTCA TGGATAGCAG TCACCTGGAG
    ATGACATCGT GGGCGGCCCT GCCCCTTCTG TCCACCAGCA CCACCAATGT CCGGAGACCC
    AAGCTCACTT TTGATGACTC GGTTCACAAT GCTGATTATT ACATGCAAGA GGCTAAGAAG
    CTGAAGCACA AAGCTGATGC ACTGTTCGAG AAATTTGGCA AAGCCGTGAA TTACGCGGAT
    GCAGCCCTGT CCTTCACTGA ATGTGGCAAC GCGATGGAGA GAGACCCTCT GGAAGCCAAG
    TCTCCATACA CCATGTACTC CGAGACTGTG GAACTCCTCA GGTATGCCAT GAGGCTGAAG
    AATTTTGCAA GCCCCCTGGC TTCAGATGGG GACAAAAAGC TGGCTGTACT ATGCTACAGA
    TGCCTGTCAC TTCTCTACCT GAGGATGTTC AAACTGAAGA AGGACCATGC CATGAAGTAC
    TCCAGATCAC TGATGGAATA TTTTAAGCAA AATGCTTCAA AAGTCGCTCA GATACCATCT
    CCATGGGTAG GCAATGGAAA GAATACTCCA TCCCCAGTGT CTCTCAACAA CGTCTCTCCC
    ATCAACACAA TGGGGAACTG TAACAACGGC CCAGTCACCA TCCCACAGCG CATTCACCAC
    ATGGCCGCCA GCCACGTCAA CATCACGAGC AATGTGTTAC GGGGCTATGA ACACTGGGAC
    ATGGCTGACA AACTGACAAG AGAGAACAAA GAATTCTTTG GCGATCTGGA CACGTTGATG
    GGGCCCCTGA CGCAGCACAG CAGCATGACC AATCTCGTCC GCTACGTTCG CCAGGGACTG
    TGCTGGCTGC GCATCGACGC CCACTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178472.1
    CDS1..3813
    Translation

    Target ORF information:

    RefSeq Version NM_001191543.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AATCCCAACA TCTGACTCCA GGATTTGCCT TACAAAAGTG GAGTGACCCG
    ACCAGCAGAG CTTCTACAAA GATGCTTGAA GATGACTTGA AGCTGAGCAG TGATGAAGAT
    GACCTTGAGC CCATGAAGAC CTTGACCACT CAGTGCACTG CCACTGAGCT CTACCAGGCT
    GTTGAAAAGG CAAAACCTAA GAATAATCCT GTGAACCCAC TCTTGGCCAC GCCCCAACCC
    CCACCTGCAG CGCCCACCAG TGGGGGATCC GGCAGCTCCA GTGACTCAGA GAGCAGCTCA
    GAGTCTGACT CAGACACTGA AAGCAGCACC ACTGACAGCG AAGCCAATGA GGCGCCGCGA
    GTGACCACTC CAGAGCCTGA GCCGCCCTCC ACTAACAAGT GGCAGTTGGA CAAATGGCTT
    AACAAAGTGA CTTCTCAAAA CAAATCTTTT ATTTGTGGCC AAAATGAAAC ACCCATGGAG
    ACGATTTCTG TGCCTCCTCC AGTCATTCAG CCAATGGAAG TCCAGGTCAA GGTGAAGGCA
    AACCCCAGCC AGGTCCTGCC TGAACCCAAA GAGAGGCCTC TCCTTGGTCT TATCAGGGAG
    AAAGCCCGTC CACGACCCAC CCTGAAAACT CCAGAAACAA AGGCTCTGAA GCATAAGTTG
    TCCACAACTG TTGAGACAGC ATCTCAAAGG ACAATTGGGA AAAAACAGCC CAAAAGGGTC
    GAGAAGAACA CCAGCGTTGA GGAATTTACC TGGCCCAAAC CAAATATCAC CAGCAGCACT
    CCCAAAGAAA AGGAAAGCGG GGAGCTTCCT GACCTCCCTA GAGGGCGCAG CAAAGCCGTT
    GCCCACAAGC CGGTTCCCAG GAAAGAACCG AGGGCCAGCA TCCCCTTGGC TCCCGAGAAG
    AAAAAGTACC GAGGGCCTGG CAAGACGGTT CCAAAGTCCC GGGAATTCAT TGAGACCGAT
    TCGTCTACAT CTGATTCCAA CACGGATCAG GAAGAGCCCC TGCAGATCAA AGTCCTGCCT
    CCATGCATGG CTCCAGGGGG CAACACGACC AAATCCAAGG AAGCCGGAGG GGCCAGCCTG
    ACCCTCAGCA GCTTTCTCAG CAGTGGCAAT GGCAACAACC CACCAGTCCC GAGTGAGGAA
    CCTACTCAGT CACCGGTCCC TGTCACACAA ACTGAGGTTC TATCTCCTGT CCGAGATCAG
    GAGAATCTGA AAAATCTCTG GGTGAAGATT GACCTTGACC TACTGTCTCG AGTGCCTGGC
    CAGAACTCAC TCCAGGCAAC GTCAGCCAAG CTAGACCACA AGGAGACAGC CTCAAAGCCC
    AAGCGGCAGA CGGCTGCGGT GCCTGCAGAG AAAGCCGCCC CCAAGGGCAA GCGTAAGCAC
    AAGCCAACAG AAGTTGCAGG GAAAATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCAGCC
    ACCATCTGCC TGCTGCCACC TTGCATCTCG CCAGCTCCCC CCCAGAAGCC TCCCAGCACT
    AAAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAAG AAGAAAAGCT GTTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGTCGCAGGA ACATCAGCGG CAATAATGGT
    CCCTTCAGTC GAGACAAAAC CATTCCTTTG ATTGGACAGA TCACCTCGAC CAAACCTAAG
    AGGAATGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAG CTGTAAACGA GGGAGACACT
    CCAAAAAAGG CAGCCCCTGG CACTGTCACC AACACTGCTA TCGCCTCTGC CACTGCCACT
    GCCATTGTCA CCAGCACTGC CACAGCCACT GCCACGGCCA CTACCACAAC CACTACCACA
    ACCATTTCCA CCACCACCTC CGCCATCACC ACTGGCCTCA TGGATAGCAG TCACCTGGAG
    ATGACATCGT GGGCGGCCCT GCCCCTTCTG TCCACCAGCA CCACCAATGT CCGGAGACCC
    AAGCTCACTT TTGATGACTC GGTTCACAAT GCTGATTATT ACATGCAAGA GGCTAAGAAG
    CTGAAGCACA AAGCTGATGC ACTGTTCGAG AAATTTGGCA AAGCCGTGAA TTACGCGGAT
    GCAGCCCTGT CCTTCACTGA ATGTGGCAAC GCGATGGAGA GAGACCCTCT GGAAGCCAAG
    TCTCCATACA CCATGTACTC CGAGACTGTG GAACTCCTCA GGTATGCCAT GAGGCTGAAG
    AATTTTGCAA GCCCCCTGGC TTCAGATGGG GACAAAAAGC TGGCTGTACT ATGCTACAGA
    TGCCTGTCAC TTCTCTACCT GAGGATGTTC AAACTGAAGA AGGACCATGC CATGAAGTAC
    TCCAGATCAC TGATGGAATA TTTTAAGCAA AATGCTTCAA AAGTCGCTCA GATACCATCT
    CCATGGGTAG GCAATGGAAA GAATACTCCA TCCCCAGTGT CTCTCAACAA CGTCTCTCCC
    ATCAACACAA TGGGGAACTG TAACAACGGC CCAGTCACCA TCCCACAGCG CATTCACCAC
    ATGGCCGCCA GCCACGTCAA CATCACGAGC AATGTGTTAC GGGGCTATGA ACACTGGGAC
    ATGGCTGACA AACTGACAAG AGAGAACAAA GAATTCTTTG GCGATCTGGA CACGTTGATG
    GGGCCCCTGA CGCAGCACAG CAGCATGACC AATCTCGTCC GCTACGTTCG CCAGGGACTG
    TGCTGGCTGC GCATCGACGC CCACTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35220
    Clone ID Related Accession (Same CDS sequence) XM_005227590.4 , XM_005227590.3
    Accession Version XM_005227590.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227590.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AGATGACCCA TCCCTGGCCT ACTCCTCTCA CTGCCATGCA TACTCCCGGA
    AATTCTGAGC AGAGCACACT TTCCATCCCA GGACAGGAAT CCCAACATCT GACTCCAGGA
    TTTGCCTTAC AAAAGTGGAG TGACCCGACC AGCAGAGCTT CTACAAAGAT GCTTGAAGAT
    GACTTGAAGC TGAGCAGTGA TGAAGATGAC CTTGAGCCCA TGAAGACCTT GACCACTCAG
    TGCACTGCCA CTGAGCTCTA CCAGGCTGTT GAAAAGGCAA AACCTAAGAA TAATCCTGTG
    AACCCACTCT TGGCCACGCC CCAACCCCCA CCTGCAGCGC CCACCAGTGG GGGATCCGGC
    AGCTCCAGTG ACTCAGAGAG CAGCTCAGAG TCTGACTCAG ACACTGAAAG CAGCACCACT
    GACAGCGAAG CCAATGAGGC GCCGCGAGTG ACCACTCCAG AGCCTGAGCC GCCCTCCACT
    AACAAGTGGC AGTTGGACAA ATGGCTTAAC AAAGTGACTT CTCAAAACAA ATCTTTTATT
    TGTGGCCAAA ATGAAACACC CATGGAGACG ATTTCTGTGC CTCCTCCAGT CATTCAGCCA
    ATGGAAGTCC AGGTCAAGGT GAAGGCAAAC CCCAGCCAGG TCCTGCCTGA ACCCAAAGAG
    AGGCCTCTCC TTGGTCTTAT CAGGGAGAAA GCCCGTCCAC GACCCACCCT GAAAACTCCA
    GAAACAAAGG CTCTGAAGCA TAAGTTGTCC ACAACTGTTG AGACAGCATC TCAAAGGACA
    ATTGGGAAAA AACAGCCCAA AAGGGTCGAG AAGAACACCA GCGTTGAGGA ATTTACCTGG
    CCCAAACCAA ATATCACCAG CAGCACTCCC AAAGAAAAGG AAAGCGGGGA GCTTCCTGAC
    CTCCCTAGAG GGCGCAGCAA AGCCGTTGCC CACAAGCCGG TTCCCAGGAA AGAACCGAGG
    GCCAGCATCC CCTTGGCTCC CGAGAAGAAA AAGTACCGAG GGCCTGGCAA GACGGTTCCA
    AAGTCCCGGG AATTCATTGA GACCGATTCG TCTACATCTG ATTCCAACAC GGATCAGGAA
    GAGCCCCTGC AGATCAAAGT CCTGCCTCCA TGCATGGCTC CAGGGGGCAA CACGACCAAA
    TCCAAGGAAG CCGGAGGGGC CAGCCTGACC CTCAGCAGCT TTCTCAGCAG TGGCAATGGC
    AACAACCCAC CAGTCCCGAG TGAGGAACCT ACTCAGTCAC CGGTCCCTGT CACACAAACT
    GAGGTTCTAT CTCCTGTCCG AGATCAGGAG AATCTGAAAA ATCTCTGGGT GAAGATTGAC
    CTTGACCTAC TGTCTCGAGT GCCTGGCCAG AACTCACTCC AGGCAACGTC AGCCAAGCTA
    GACCACAAGG AGACAGCCTC AAAGCCCAAG CGGCAGACGG CTGCGGTGCC TGCAGAGAAA
    GCCGCCCCCA AGGGCAAGCG TAAGCACAAG CCAACAGAAG TTGCAGGGAA AATCCCTGAG
    AAGAAGCAGC GCCTGGAGGA GGCAGCCACC ATCTGCCTGC TGCCACCTTG CATCTCGCCA
    GCTCCCCCCC AGAAGCCTCC CAGCACTAAA GAAAATAATT CATCCAGGAG AGCAAATAGA
    AGAAAAGAAG AAAAGCTGTT TCCTCCTCCA CTTTCCCCAC TGCCAGAGGA CCCTCCACGT
    CGCAGGAACA TCAGCGGCAA TAATGGTCCC TTCAGTCGAG ACAAAACCAT TCCTTTGATT
    GGACAGATCA CCTCGACCAA ACCTAAGAGG AATGAAGGCA AATTCTGTGC TACTTTCAAA
    GGGATAGCTG TAAACGAGGG AGACACTCCA AAAAAGGCAG CCCCTGGCAC TGTCACCAAC
    ACTGCTATCG CCTCTGCCAC TGCCACTGCC ATTGTCACCA GCACTGCCAC AGCCACTGCC
    ACGGCCACTA CCACAACCAC TACCACAACC ATTTCCACCA CCACCTCCGC CATCACCACT
    GGCCTCATGG ATAGCAGTCA CCTGGAGATG ACATCGTGGG CGGCCCTGCC CCTTCTGTCC
    ACCAGCACCA CCAATGTCCG GAGACCCAAG CTCACTTTTG ATGACTCGGT TCACAATGCT
    GATTATTACA TGCAAGAGGC TAAGAAGCTG AAGCACAAAG CTGATGCACT GTTCGAGAAA
    TTTGGCAAAG CCGTGAATTA CGCGGATGCA GCCCTGTCCT TCACTGAATG TGGCAACGCG
    ATGGAGAGAG ACCCTCTGGA AGCCAAGTCT CCATACACCA TGTACTCCGA GACTGTGGAA
    CTCCTCAGGT ATGCCATGAG GCTGAAGAAT TTTGCAAGCC CCCTGGCTTC AGATGGGGAC
    AAAAAGCTGG CTGTACTATG CTACAGATGC CTGTCACTTC TCTACCTGAG GATGTTCAAA
    CTGAAGAAGG ACCATGCCAT GAAGTACTCC AGATCACTGA TGGAATATTT TAAGCAAAAT
    GCTTCAAAAG TCGCTCAGAT ACCATCTCCA TGGGTAGGCA ATGGAAAGAA TACTCCATCC
    CCAGTGTCTC TCAACAACGT CTCTCCCATC AACACAATGG GGAACTGTAA CAACGGCCCA
    GTCACCATCC CACAGCGCAT TCACCACATG GCCGCCAGCC ACGTCAACAT CACGAGCAAT
    GTGTTACGGG GCTATGAACA CTGGGACATG GCTGACAAAC TGACAAGAGA GAACAAAGAA
    TTCTTTGGCG ATCTGGACAC GTTGATGGGG CCCCTGACGC AGCACAGCAG CATGACCAAT
    CTCGTCCGCT ACGTTCGCCA GGGACTGTGC TGGCTGCGCA TCGACGCCCA CTTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227647.1
    CDS276..4175
    Translation

    Target ORF information:

    RefSeq Version XM_005227590.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227590.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AGATGACCCA TCCCTGGCCT ACTCCTCTCA CTGCCATGCA TACTCCCGGA
    AATTCTGAGC AGAGCACACT TTCCATCCCA GGACAGGAAT CCCAACATCT GACTCCAGGA
    TTTGCCTTAC AAAAGTGGAG TGACCCGACC AGCAGAGCTT CTACAAAGAT GCTTGAAGAT
    GACTTGAAGC TGAGCAGTGA TGAAGATGAC CTTGAGCCCA TGAAGACCTT GACCACTCAG
    TGCACTGCCA CTGAGCTCTA CCAGGCTGTT GAAAAGGCAA AACCTAAGAA TAATCCTGTG
    AACCCACTCT TGGCCACGCC CCAACCCCCA CCTGCAGCGC CCACCAGTGG GGGATCCGGC
    AGCTCCAGTG ACTCAGAGAG CAGCTCAGAG TCTGACTCAG ACACTGAAAG CAGCACCACT
    GACAGCGAAG CCAATGAGGC GCCGCGAGTG ACCACTCCAG AGCCTGAGCC GCCCTCCACT
    AACAAGTGGC AGTTGGACAA ATGGCTTAAC AAAGTGACTT CTCAAAACAA ATCTTTTATT
    TGTGGCCAAA ATGAAACACC CATGGAGACG ATTTCTGTGC CTCCTCCAGT CATTCAGCCA
    ATGGAAGTCC AGGTCAAGGT GAAGGCAAAC CCCAGCCAGG TCCTGCCTGA ACCCAAAGAG
    AGGCCTCTCC TTGGTCTTAT CAGGGAGAAA GCCCGTCCAC GACCCACCCT GAAAACTCCA
    GAAACAAAGG CTCTGAAGCA TAAGTTGTCC ACAACTGTTG AGACAGCATC TCAAAGGACA
    ATTGGGAAAA AACAGCCCAA AAGGGTCGAG AAGAACACCA GCGTTGAGGA ATTTACCTGG
    CCCAAACCAA ATATCACCAG CAGCACTCCC AAAGAAAAGG AAAGCGGGGA GCTTCCTGAC
    CTCCCTAGAG GGCGCAGCAA AGCCGTTGCC CACAAGCCGG TTCCCAGGAA AGAACCGAGG
    GCCAGCATCC CCTTGGCTCC CGAGAAGAAA AAGTACCGAG GGCCTGGCAA GACGGTTCCA
    AAGTCCCGGG AATTCATTGA GACCGATTCG TCTACATCTG ATTCCAACAC GGATCAGGAA
    GAGCCCCTGC AGATCAAAGT CCTGCCTCCA TGCATGGCTC CAGGGGGCAA CACGACCAAA
    TCCAAGGAAG CCGGAGGGGC CAGCCTGACC CTCAGCAGCT TTCTCAGCAG TGGCAATGGC
    AACAACCCAC CAGTCCCGAG TGAGGAACCT ACTCAGTCAC CGGTCCCTGT CACACAAACT
    GAGGTTCTAT CTCCTGTCCG AGATCAGGAG AATCTGAAAA ATCTCTGGGT GAAGATTGAC
    CTTGACCTAC TGTCTCGAGT GCCTGGCCAG AACTCACTCC AGGCAACGTC AGCCAAGCTA
    GACCACAAGG AGACAGCCTC AAAGCCCAAG CGGCAGACGG CTGCGGTGCC TGCAGAGAAA
    GCCGCCCCCA AGGGCAAGCG TAAGCACAAG CCAACAGAAG TTGCAGGGAA AATCCCTGAG
    AAGAAGCAGC GCCTGGAGGA GGCAGCCACC ATCTGCCTGC TGCCACCTTG CATCTCGCCA
    GCTCCCCCCC AGAAGCCTCC CAGCACTAAA GAAAATAATT CATCCAGGAG AGCAAATAGA
    AGAAAAGAAG AAAAGCTGTT TCCTCCTCCA CTTTCCCCAC TGCCAGAGGA CCCTCCACGT
    CGCAGGAACA TCAGCGGCAA TAATGGTCCC TTCAGTCGAG ACAAAACCAT TCCTTTGATT
    GGACAGATCA CCTCGACCAA ACCTAAGAGG AATGAAGGCA AATTCTGTGC TACTTTCAAA
    GGGATAGCTG TAAACGAGGG AGACACTCCA AAAAAGGCAG CCCCTGGCAC TGTCACCAAC
    ACTGCTATCG CCTCTGCCAC TGCCACTGCC ATTGTCACCA GCACTGCCAC AGCCACTGCC
    ACGGCCACTA CCACAACCAC TACCACAACC ATTTCCACCA CCACCTCCGC CATCACCACT
    GGCCTCATGG ATAGCAGTCA CCTGGAGATG ACATCGTGGG CGGCCCTGCC CCTTCTGTCC
    ACCAGCACCA CCAATGTCCG GAGACCCAAG CTCACTTTTG ATGACTCGGT TCACAATGCT
    GATTATTACA TGCAAGAGGC TAAGAAGCTG AAGCACAAAG CTGATGCACT GTTCGAGAAA
    TTTGGCAAAG CCGTGAATTA CGCGGATGCA GCCCTGTCCT TCACTGAATG TGGCAACGCG
    ATGGAGAGAG ACCCTCTGGA AGCCAAGTCT CCATACACCA TGTACTCCGA GACTGTGGAA
    CTCCTCAGGT ATGCCATGAG GCTGAAGAAT TTTGCAAGCC CCCTGGCTTC AGATGGGGAC
    AAAAAGCTGG CTGTACTATG CTACAGATGC CTGTCACTTC TCTACCTGAG GATGTTCAAA
    CTGAAGAAGG ACCATGCCAT GAAGTACTCC AGATCACTGA TGGAATATTT TAAGCAAAAT
    GCTTCAAAAG TCGCTCAGAT ACCATCTCCA TGGGTAGGCA ATGGAAAGAA TACTCCATCC
    CCAGTGTCTC TCAACAACGT CTCTCCCATC AACACAATGG GGAACTGTAA CAACGGCCCA
    GTCACCATCC CACAGCGCAT TCACCACATG GCCGCCAGCC ACGTCAACAT CACGAGCAAT
    GTGTTACGGG GCTATGAACA CTGGGACATG GCTGACAAAC TGACAAGAGA GAACAAAGAA
    TTCTTTGGCG ATCTGGACAC GTTGATGGGG CCCCTGACGC AGCACAGCAG CATGACCAAT
    CTCGTCCGCT ACGTTCGCCA GGGACTGTGC TGGCTGCGCA TCGACGCCCA CTTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35220
    Clone ID Related Accession (Same CDS sequence) XM_005227590.4 , XM_005227590.3
    Accession Version XM_005227590.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104664.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AGATGACCCA TCCCTGGCCT ACTCCTCTCA CTGCCATGCA TACTCCCGGA
    AATTCTGAGC AGAGCACACT TTCCATCCCA GGACAGGAAT CCCAACATCT GACTCCAGGA
    TTTGCCTTAC AAAAGTGGAG TGACCCGACC AGCAGAGCTT CTACAAAGAT GCTTGAAGAT
    GACTTGAAGC TGAGCAGTGA TGAAGATGAC CTTGAGCCCA TGAAGACCTT GACCACTCAG
    TGCACTGCCA CTGAGCTCTA CCAGGCTGTT GAAAAGGCAA AACCTAAGAA TAATCCTGTG
    AACCCACTCT TGGCCACGCC CCAACCCCCA CCTGCAGCGC CCACCAGTGG GGGATCCGGC
    AGCTCCAGTG ACTCAGAGAG CAGCTCAGAG TCTGACTCAG ACACTGAAAG CAGCACCACT
    GACAGCGAAG CCAATGAGGC GCCGCGAGTG ACCACTCCAG AGCCTGAGCC GCCCTCCACT
    AACAAGTGGC AGTTGGACAA ATGGCTTAAC AAAGTGACTT CTCAAAACAA ATCTTTTATT
    TGTGGCCAAA ATGAAACACC CATGGAGACG ATTTCTGTGC CTCCTCCAGT CATTCAGCCA
    ATGGAAGTCC AGGTCAAGGT GAAGGCAAAC CCCAGCCAGG TCCTGCCTGA ACCCAAAGAG
    AGGCCTCTCC TTGGTCTTAT CAGGGAGAAA GCCCGTCCAC GACCCACCCT GAAAACTCCA
    GAAACAAAGG CTCTGAAGCA TAAGTTGTCC ACAACTGTTG AGACAGCATC TCAAAGGACA
    ATTGGGAAAA AACAGCCCAA AAGGGTCGAG AAGAACACCA GCGTTGAGGA ATTTACCTGG
    CCCAAACCAA ATATCACCAG CAGCACTCCC AAAGAAAAGG AAAGCGGGGA GCTTCCTGAC
    CTCCCTAGAG GGCGCAGCAA AGCCGTTGCC CACAAGCCGG TTCCCAGGAA AGAACCGAGG
    GCCAGCATCC CCTTGGCTCC CGAGAAGAAA AAGTACCGAG GGCCTGGCAA GACGGTTCCA
    AAGTCCCGGG AATTCATTGA GACCGATTCG TCTACATCTG ATTCCAACAC GGATCAGGAA
    GAGCCCCTGC AGATCAAAGT CCTGCCTCCA TGCATGGCTC CAGGGGGCAA CACGACCAAA
    TCCAAGGAAG CCGGAGGGGC CAGCCTGACC CTCAGCAGCT TTCTCAGCAG TGGCAATGGC
    AACAACCCAC CAGTCCCGAG TGAGGAACCT ACTCAGTCAC CGGTCCCTGT CACACAAACT
    GAGGTTCTAT CTCCTGTCCG AGATCAGGAG AATCTGAAAA ATCTCTGGGT GAAGATTGAC
    CTTGACCTAC TGTCTCGAGT GCCTGGCCAG AACTCACTCC AGGCAACGTC AGCCAAGCTA
    GACCACAAGG AGACAGCCTC AAAGCCCAAG CGGCAGACGG CTGCGGTGCC TGCAGAGAAA
    GCCGCCCCCA AGGGCAAGCG TAAGCACAAG CCAACAGAAG TTGCAGGGAA AATCCCTGAG
    AAGAAGCAGC GCCTGGAGGA GGCAGCCACC ATCTGCCTGC TGCCACCTTG CATCTCGCCA
    GCTCCCCCCC AGAAGCCTCC CAGCACTAAA GAAAATAATT CATCCAGGAG AGCAAATAGA
    AGAAAAGAAG AAAAGCTGTT TCCTCCTCCA CTTTCCCCAC TGCCAGAGGA CCCTCCACGT
    CGCAGGAACA TCAGCGGCAA TAATGGTCCC TTCAGTCGAG ACAAAACCAT TCCTTTGATT
    GGACAGATCA CCTCGACCAA ACCTAAGAGG AATGAAGGCA AATTCTGTGC TACTTTCAAA
    GGGATAGCTG TAAACGAGGG AGACACTCCA AAAAAGGCAG CCCCTGGCAC TGTCACCAAC
    ACTGCTATCG CCTCTGCCAC TGCCACTGCC ATTGTCACCA GCACTGCCAC AGCCACTGCC
    ACGGCCACTA CCACAACCAC TACCACAACC ATTTCCACCA CCACCTCCGC CATCACCACT
    GGCCTCATGG ATAGCAGTCA CCTGGAGATG ACATCGTGGG CGGCCCTGCC CCTTCTGTCC
    ACCAGCACCA CCAATGTCCG GAGACCCAAG CTCACTTTTG ATGACTCGGT TCACAATGCT
    GATTATTACA TGCAAGAGGC TAAGAAGCTG AAGCACAAAG CTGATGCACT GTTCGAGAAA
    TTTGGCAAAG CCGTGAATTA CGCGGATGCA GCCCTGTCCT TCACTGAATG TGGCAACGCG
    ATGGAGAGAG ACCCTCTGGA AGCCAAGTCT CCATACACCA TGTACTCCGA GACTGTGGAA
    CTCCTCAGGT ATGCCATGAG GCTGAAGAAT TTTGCAAGCC CCCTGGCTTC AGATGGGGAC
    AAAAAGCTGG CTGTACTATG CTACAGATGC CTGTCACTTC TCTACCTGAG GATGTTCAAA
    CTGAAGAAGG ACCATGCCAT GAAGTACTCC AGATCACTGA TGGAATATTT TAAGCAAAAT
    GCTTCAAAAG TCGCTCAGAT ACCATCTCCA TGGGTAGGCA ATGGAAAGAA TACTCCATCC
    CCAGTGTCTC TCAACAACGT CTCTCCCATC AACACAATGG GGAACTGTAA CAACGGCCCA
    GTCACCATCC CACAGCGCAT TCACCACATG GCCGCCAGCC ACGTCAACAT CACGAGCAAT
    GTGTTACGGG GCTATGAACA CTGGGACATG GCTGACAAAC TGACAAGAGA GAACAAAGAA
    TTCTTTGGCG ATCTGGACAC GTTGATGGGG CCCCTGACGC AGCACAGCAG CATGACCAAT
    CTCGTCCGCT ACGTTCGCCA GGGACTGTGC TGGCTGCGCA TCGACGCCCA CTTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227647.1
    CDS67..3966
    Translation

    Target ORF information:

    RefSeq Version XM_005227590.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227590.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT CGCGAACCGA GTCCAGAACA CGCTGGGAAA TTACGATGAA
    ATGAAGGATT TGCTAACTAA CCATTCTAAT CAGAATCATC TAGTGGGAAT CCCAAAGAAT
    TCCATGCCCC AGACTCCCAC CAACAAAAAT GAACCCAGCT TTTTTCCAGA ACAAAAGCAC
    CGAATGGTCC CAGTGCACCA GCAGGACAAC ACTCACTCCT CGGCGCCAAT GCCTCCACCT
    TCTGTCGTGA TACTGAATTC AACTCTAATA CACAGCAACC GGAAGTCAAA ACCTGACTGG
    TCACGAGATA GTCAGAACAC TAGCATTGTA GCAGCCAGCC AAGTCACTAG TCAGCCCAAC
    AAGATGCAGC CGCCAACCCC AGACCAGCCC CCAGCCAGAT TGGAAGACTT CTTTGTCTAC
    CCAGCTGAAC AGCCTCAAGT TGGAGCAGTT GAAGAATCAA ACCCACTGGC AAAGGAAGAC
    AATACTCTCA AGTCCAGTGG AGGGGATACT TTCAAAGAAA TCTATCAGTC CAACTCACCA
    GAAGCAGCGG AATTTACAGT GCAAGCGCCT GGATCTCCCC TAGTTGCCTC GTCTCTATTA
    GCTCCCAGCA GTGGCCTTTC GGTTCAAAAC TTCCCACCAG GGCTTTACTG CAAAACAAGC
    ATGGGGCAGC AAAAACCAAC CGCCTATGTC CGACCCATGG ATGGTCAGGA CCAGGCCCCG
    GACATCTCTC CCACACTGAA ACCTTCGATT GAATTTGACA ACAGCTTTGG GAATCTGTCG
    TTTGGATCAC TCTTGGATGG CAAACCCAGT GCACCCAGTT CAAAGACTAA ACAGCCAAAG
    TTCACGATTC TTCAAACAAG TGAAGTAAGC CTTCCCAGTG ACCCAAGCTG TGTTGAAGAA
    ATCTTGCGGG AGATGACCCA TCCCTGGCCT ACTCCTCTCA CTGCCATGCA TACTCCCGGA
    AATTCTGAGC AGAGCACACT TTCCATCCCA GGACAGGAAT CCCAACATCT GACTCCAGGA
    TTTGCCTTAC AAAAGTGGAG TGACCCGACC AGCAGAGCTT CTACAAAGAT GCTTGAAGAT
    GACTTGAAGC TGAGCAGTGA TGAAGATGAC CTTGAGCCCA TGAAGACCTT GACCACTCAG
    TGCACTGCCA CTGAGCTCTA CCAGGCTGTT GAAAAGGCAA AACCTAAGAA TAATCCTGTG
    AACCCACTCT TGGCCACGCC CCAACCCCCA CCTGCAGCGC CCACCAGTGG GGGATCCGGC
    AGCTCCAGTG ACTCAGAGAG CAGCTCAGAG TCTGACTCAG ACACTGAAAG CAGCACCACT
    GACAGCGAAG CCAATGAGGC GCCGCGAGTG ACCACTCCAG AGCCTGAGCC GCCCTCCACT
    AACAAGTGGC AGTTGGACAA ATGGCTTAAC AAAGTGACTT CTCAAAACAA ATCTTTTATT
    TGTGGCCAAA ATGAAACACC CATGGAGACG ATTTCTGTGC CTCCTCCAGT CATTCAGCCA
    ATGGAAGTCC AGGTCAAGGT GAAGGCAAAC CCCAGCCAGG TCCTGCCTGA ACCCAAAGAG
    AGGCCTCTCC TTGGTCTTAT CAGGGAGAAA GCCCGTCCAC GACCCACCCT GAAAACTCCA
    GAAACAAAGG CTCTGAAGCA TAAGTTGTCC ACAACTGTTG AGACAGCATC TCAAAGGACA
    ATTGGGAAAA AACAGCCCAA AAGGGTCGAG AAGAACACCA GCGTTGAGGA ATTTACCTGG
    CCCAAACCAA ATATCACCAG CAGCACTCCC AAAGAAAAGG AAAGCGGGGA GCTTCCTGAC
    CTCCCTAGAG GGCGCAGCAA AGCCGTTGCC CACAAGCCGG TTCCCAGGAA AGAACCGAGG
    GCCAGCATCC CCTTGGCTCC CGAGAAGAAA AAGTACCGAG GGCCTGGCAA GACGGTTCCA
    AAGTCCCGGG AATTCATTGA GACCGATTCG TCTACATCTG ATTCCAACAC GGATCAGGAA
    GAGCCCCTGC AGATCAAAGT CCTGCCTCCA TGCATGGCTC CAGGGGGCAA CACGACCAAA
    TCCAAGGAAG CCGGAGGGGC CAGCCTGACC CTCAGCAGCT TTCTCAGCAG TGGCAATGGC
    AACAACCCAC CAGTCCCGAG TGAGGAACCT ACTCAGTCAC CGGTCCCTGT CACACAAACT
    GAGGTTCTAT CTCCTGTCCG AGATCAGGAG AATCTGAAAA ATCTCTGGGT GAAGATTGAC
    CTTGACCTAC TGTCTCGAGT GCCTGGCCAG AACTCACTCC AGGCAACGTC AGCCAAGCTA
    GACCACAAGG AGACAGCCTC AAAGCCCAAG CGGCAGACGG CTGCGGTGCC TGCAGAGAAA
    GCCGCCCCCA AGGGCAAGCG TAAGCACAAG CCAACAGAAG TTGCAGGGAA AATCCCTGAG
    AAGAAGCAGC GCCTGGAGGA GGCAGCCACC ATCTGCCTGC TGCCACCTTG CATCTCGCCA
    GCTCCCCCCC AGAAGCCTCC CAGCACTAAA GAAAATAATT CATCCAGGAG AGCAAATAGA
    AGAAAAGAAG AAAAGCTGTT TCCTCCTCCA CTTTCCCCAC TGCCAGAGGA CCCTCCACGT
    CGCAGGAACA TCAGCGGCAA TAATGGTCCC TTCAGTCGAG ACAAAACCAT TCCTTTGATT
    GGACAGATCA CCTCGACCAA ACCTAAGAGG AATGAAGGCA AATTCTGTGC TACTTTCAAA
    GGGATAGCTG TAAACGAGGG AGACACTCCA AAAAAGGCAG CCCCTGGCAC TGTCACCAAC
    ACTGCTATCG CCTCTGCCAC TGCCACTGCC ATTGTCACCA GCACTGCCAC AGCCACTGCC
    ACGGCCACTA CCACAACCAC TACCACAACC ATTTCCACCA CCACCTCCGC CATCACCACT
    GGCCTCATGG ATAGCAGTCA CCTGGAGATG ACATCGTGGG CGGCCCTGCC CCTTCTGTCC
    ACCAGCACCA CCAATGTCCG GAGACCCAAG CTCACTTTTG ATGACTCGGT TCACAATGCT
    GATTATTACA TGCAAGAGGC TAAGAAGCTG AAGCACAAAG CTGATGCACT GTTCGAGAAA
    TTTGGCAAAG CCGTGAATTA CGCGGATGCA GCCCTGTCCT TCACTGAATG TGGCAACGCG
    ATGGAGAGAG ACCCTCTGGA AGCCAAGTCT CCATACACCA TGTACTCCGA GACTGTGGAA
    CTCCTCAGGT ATGCCATGAG GCTGAAGAAT TTTGCAAGCC CCCTGGCTTC AGATGGGGAC
    AAAAAGCTGG CTGTACTATG CTACAGATGC CTGTCACTTC TCTACCTGAG GATGTTCAAA
    CTGAAGAAGG ACCATGCCAT GAAGTACTCC AGATCACTGA TGGAATATTT TAAGCAAAAT
    GCTTCAAAAG TCGCTCAGAT ACCATCTCCA TGGGTAGGCA ATGGAAAGAA TACTCCATCC
    CCAGTGTCTC TCAACAACGT CTCTCCCATC AACACAATGG GGAACTGTAA CAACGGCCCA
    GTCACCATCC CACAGCGCAT TCACCACATG GCCGCCAGCC ACGTCAACAT CACGAGCAAT
    GTGTTACGGG GCTATGAACA CTGGGACATG GCTGACAAAC TGACAAGAGA GAACAAAGAA
    TTCTTTGGCG ATCTGGACAC GTTGATGGGG CCCCTGACGC AGCACAGCAG CATGACCAAT
    CTCGTCCGCT ACGTTCGCCA GGGACTGTGC TGGCTGCGCA TCGACGCCCA CTTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35221
    Clone ID Related Accession (Same CDS sequence) XM_005227591.4 , XM_005227591.3
    Accession Version XM_005227591.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227591.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAG
    ATGACCCATC CCTGGCCTAC TCCTCTCACT GCCATGCATA CTCCCGGAAA TTCTGAGCAG
    AGCACACTTT CCATCCCAGG ACAGGAATCC CAACATCTGA CTCCAGGATT TGCCTTACAA
    AAGTGGAGTG ACCCGACCAG CAGAGCTTCT ACAAAGATGC TTGAAGATGA CTTGAAGCTG
    AGCAGTGATG AAGATGACCT TGAGCCCATG AAGACCTTGA CCACTCAGTG CACTGCCACT
    GAGCTCTACC AGGCTGTTGA AAAGGCAAAA CCTAAGAATA ATCCTGTGAA CCCACTCTTG
    GCCACGCCCC AACCCCCACC TGCAGCGCCC ACCAGTGGGG GATCCGGCAG CTCCAGTGAC
    TCAGAGAGCA GCTCAGAGTC TGACTCAGAC ACTGAAAGCA GCACCACTGA CAGCGAAGCC
    AATGAGGCGC CGCGAGTGAC CACTCCAGAG CCTGAGCCGC CCTCCACTAA CAAGTGGCAG
    TTGGACAAAT GGCTTAACAA AGTGACTTCT CAAAACAAAT CTTTTATTTG TGGCCAAAAT
    GAAACACCCA TGGAGACGAT TTCTGTGCCT CCTCCAGTCA TTCAGCCAAT GGAAGTCCAG
    GTCAAGGTGA AGGCAAACCC CAGCCAGGTC CTGCCTGAAC CCAAAGAGAG GCCTCTCCTT
    GGTCTTATCA GGGAGAAAGC CCGTCCACGA CCCACCCTGA AAACTCCAGA AACAAAGGCT
    CTGAAGCATA AGTTGTCCAC AACTGTTGAG ACAGCATCTC AAAGGACAAT TGGGAAAAAA
    CAGCCCAAAA GGGTCGAGAA GAACACCAGC GTTGAGGAAT TTACCTGGCC CAAACCAAAT
    ATCACCAGCA GCACTCCCAA AGAAAAGGAA AGCGGGGAGC TTCCTGACCT CCCTAGAGGG
    CGCAGCAAAG CCGTTGCCCA CAAGCCGGTT CCCAGGAAAG AACCGAGGGC CAGCATCCCC
    TTGGCTCCCG AGAAGAAAAA GTACCGAGGG CCTGGCAAGA CGGTTCCAAA GTCCCGGGAA
    TTCATTGAGA CCGATTCGTC TACATCTGAT TCCAACACGG ATCAGGAAGA GCCCCTGCAG
    ATCAAAGTCC TGCCTCCATG CATGGCTCCA GGGGGCAACA CGACCAAATC CAAGGAAGCC
    GGAGGGGCCA GCCTGACCCT CAGCAGCTTT CTCAGCAGTG GCAATGGCAA CAACCCACCA
    GTCCCGAGTG AGGAACCTAC TCAGTCACCG GTCCCTGTCA CACAAACTGA GGTTCTATCT
    CCTGTCCGAG ATCAGGAGAA TCTGAAAAAT CTCTGGGTGA AGATTGACCT TGACCTACTG
    TCTCGAGTGC CTGGCCAGAA CTCACTCCAG GCAACGTCAG CCAAGCTAGA CCACAAGGAG
    ACAGCCTCAA AGCCCAAGCG GCAGACGGCT GCGGTGCCTG CAGAGAAAGC CGCCCCCAAG
    GGCAAGCGTA AGCACAAGCC AACAGAAGTT GCAGGGAAAA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CAGCCACCAT CTGCCTGCTG CCACCTTGCA TCTCGCCAGC TCCCCCCCAG
    AAGCCTCCCA GCACTAAAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAAGAAGAA
    AAGCTGTTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGTCG CAGGAACATC
    AGCGGCAATA ATGGTCCCTT CAGTCGAGAC AAAACCATTC CTTTGATTGG ACAGATCACC
    TCGACCAAAC CTAAGAGGAA TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATAGCTGTA
    AACGAGGGAG ACACTCCAAA AAAGGCAGCC CCTGGCACTG TCACCAACAC TGCTATCGCC
    TCTGCCACTG CCACTGCCAT TGTCACCAGC ACTGCCACAG CCACTGCCAC GGCCACTACC
    ACAACCACTA CCACAACCAT TTCCACCACC ACCTCCGCCA TCACCACTGG CCTCATGGAT
    AGCAGTCACC TGGAGATGAC ATCGTGGGCG GCCCTGCCCC TTCTGTCCAC CAGCACCACC
    AATGTCCGGA GACCCAAGCT CACTTTTGAT GACTCGGTTC ACAATGCTGA TTATTACATG
    CAAGAGGCTA AGAAGCTGAA GCACAAAGCT GATGCACTGT TCGAGAAATT TGGCAAAGCC
    GTGAATTACG CGGATGCAGC CCTGTCCTTC ACTGAATGTG GCAACGCGAT GGAGAGAGAC
    CCTCTGGAAG CCAAGTCTCC ATACACCATG TACTCCGAGA CTGTGGAACT CCTCAGGTAT
    GCCATGAGGC TGAAGAATTT TGCAAGCCCC CTGGCTTCAG ATGGGGACAA AAAGCTGGCT
    GTACTATGCT ACAGATGCCT GTCACTTCTC TACCTGAGGA TGTTCAAACT GAAGAAGGAC
    CATGCCATGA AGTACTCCAG ATCACTGATG GAATATTTTA AGCAAAATGC TTCAAAAGTC
    GCTCAGATAC CATCTCCATG GGTAGGCAAT GGAAAGAATA CTCCATCCCC AGTGTCTCTC
    AACAACGTCT CTCCCATCAA CACAATGGGG AACTGTAACA ACGGCCCAGT CACCATCCCA
    CAGCGCATTC ACCACATGGC CGCCAGCCAC GTCAACATCA CGAGCAATGT GTTACGGGGC
    TATGAACACT GGGACATGGC TGACAAACTG ACAAGAGAGA ACAAAGAATT CTTTGGCGAT
    CTGGACACGT TGATGGGGCC CCTGACGCAG CACAGCAGCA TGACCAATCT CGTCCGCTAC
    GTTCGCCAGG GACTGTGCTG GCTGCGCATC GACGCCCACT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227648.1
    CDS276..4163
    Translation

    Target ORF information:

    RefSeq Version XM_005227591.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227591.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAG
    ATGACCCATC CCTGGCCTAC TCCTCTCACT GCCATGCATA CTCCCGGAAA TTCTGAGCAG
    AGCACACTTT CCATCCCAGG ACAGGAATCC CAACATCTGA CTCCAGGATT TGCCTTACAA
    AAGTGGAGTG ACCCGACCAG CAGAGCTTCT ACAAAGATGC TTGAAGATGA CTTGAAGCTG
    AGCAGTGATG AAGATGACCT TGAGCCCATG AAGACCTTGA CCACTCAGTG CACTGCCACT
    GAGCTCTACC AGGCTGTTGA AAAGGCAAAA CCTAAGAATA ATCCTGTGAA CCCACTCTTG
    GCCACGCCCC AACCCCCACC TGCAGCGCCC ACCAGTGGGG GATCCGGCAG CTCCAGTGAC
    TCAGAGAGCA GCTCAGAGTC TGACTCAGAC ACTGAAAGCA GCACCACTGA CAGCGAAGCC
    AATGAGGCGC CGCGAGTGAC CACTCCAGAG CCTGAGCCGC CCTCCACTAA CAAGTGGCAG
    TTGGACAAAT GGCTTAACAA AGTGACTTCT CAAAACAAAT CTTTTATTTG TGGCCAAAAT
    GAAACACCCA TGGAGACGAT TTCTGTGCCT CCTCCAGTCA TTCAGCCAAT GGAAGTCCAG
    GTCAAGGTGA AGGCAAACCC CAGCCAGGTC CTGCCTGAAC CCAAAGAGAG GCCTCTCCTT
    GGTCTTATCA GGGAGAAAGC CCGTCCACGA CCCACCCTGA AAACTCCAGA AACAAAGGCT
    CTGAAGCATA AGTTGTCCAC AACTGTTGAG ACAGCATCTC AAAGGACAAT TGGGAAAAAA
    CAGCCCAAAA GGGTCGAGAA GAACACCAGC GTTGAGGAAT TTACCTGGCC CAAACCAAAT
    ATCACCAGCA GCACTCCCAA AGAAAAGGAA AGCGGGGAGC TTCCTGACCT CCCTAGAGGG
    CGCAGCAAAG CCGTTGCCCA CAAGCCGGTT CCCAGGAAAG AACCGAGGGC CAGCATCCCC
    TTGGCTCCCG AGAAGAAAAA GTACCGAGGG CCTGGCAAGA CGGTTCCAAA GTCCCGGGAA
    TTCATTGAGA CCGATTCGTC TACATCTGAT TCCAACACGG ATCAGGAAGA GCCCCTGCAG
    ATCAAAGTCC TGCCTCCATG CATGGCTCCA GGGGGCAACA CGACCAAATC CAAGGAAGCC
    GGAGGGGCCA GCCTGACCCT CAGCAGCTTT CTCAGCAGTG GCAATGGCAA CAACCCACCA
    GTCCCGAGTG AGGAACCTAC TCAGTCACCG GTCCCTGTCA CACAAACTGA GGTTCTATCT
    CCTGTCCGAG ATCAGGAGAA TCTGAAAAAT CTCTGGGTGA AGATTGACCT TGACCTACTG
    TCTCGAGTGC CTGGCCAGAA CTCACTCCAG GCAACGTCAG CCAAGCTAGA CCACAAGGAG
    ACAGCCTCAA AGCCCAAGCG GCAGACGGCT GCGGTGCCTG CAGAGAAAGC CGCCCCCAAG
    GGCAAGCGTA AGCACAAGCC AACAGAAGTT GCAGGGAAAA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CAGCCACCAT CTGCCTGCTG CCACCTTGCA TCTCGCCAGC TCCCCCCCAG
    AAGCCTCCCA GCACTAAAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAAGAAGAA
    AAGCTGTTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGTCG CAGGAACATC
    AGCGGCAATA ATGGTCCCTT CAGTCGAGAC AAAACCATTC CTTTGATTGG ACAGATCACC
    TCGACCAAAC CTAAGAGGAA TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATAGCTGTA
    AACGAGGGAG ACACTCCAAA AAAGGCAGCC CCTGGCACTG TCACCAACAC TGCTATCGCC
    TCTGCCACTG CCACTGCCAT TGTCACCAGC ACTGCCACAG CCACTGCCAC GGCCACTACC
    ACAACCACTA CCACAACCAT TTCCACCACC ACCTCCGCCA TCACCACTGG CCTCATGGAT
    AGCAGTCACC TGGAGATGAC ATCGTGGGCG GCCCTGCCCC TTCTGTCCAC CAGCACCACC
    AATGTCCGGA GACCCAAGCT CACTTTTGAT GACTCGGTTC ACAATGCTGA TTATTACATG
    CAAGAGGCTA AGAAGCTGAA GCACAAAGCT GATGCACTGT TCGAGAAATT TGGCAAAGCC
    GTGAATTACG CGGATGCAGC CCTGTCCTTC ACTGAATGTG GCAACGCGAT GGAGAGAGAC
    CCTCTGGAAG CCAAGTCTCC ATACACCATG TACTCCGAGA CTGTGGAACT CCTCAGGTAT
    GCCATGAGGC TGAAGAATTT TGCAAGCCCC CTGGCTTCAG ATGGGGACAA AAAGCTGGCT
    GTACTATGCT ACAGATGCCT GTCACTTCTC TACCTGAGGA TGTTCAAACT GAAGAAGGAC
    CATGCCATGA AGTACTCCAG ATCACTGATG GAATATTTTA AGCAAAATGC TTCAAAAGTC
    GCTCAGATAC CATCTCCATG GGTAGGCAAT GGAAAGAATA CTCCATCCCC AGTGTCTCTC
    AACAACGTCT CTCCCATCAA CACAATGGGG AACTGTAACA ACGGCCCAGT CACCATCCCA
    CAGCGCATTC ACCACATGGC CGCCAGCCAC GTCAACATCA CGAGCAATGT GTTACGGGGC
    TATGAACACT GGGACATGGC TGACAAACTG ACAAGAGAGA ACAAAGAATT CTTTGGCGAT
    CTGGACACGT TGATGGGGCC CCTGACGCAG CACAGCAGCA TGACCAATCT CGTCCGCTAC
    GTTCGCCAGG GACTGTGCTG GCTGCGCATC GACGCCCACT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35221
    Clone ID Related Accession (Same CDS sequence) XM_005227591.4 , XM_005227591.3
    Accession Version XM_005227591.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104664.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAG
    ATGACCCATC CCTGGCCTAC TCCTCTCACT GCCATGCATA CTCCCGGAAA TTCTGAGCAG
    AGCACACTTT CCATCCCAGG ACAGGAATCC CAACATCTGA CTCCAGGATT TGCCTTACAA
    AAGTGGAGTG ACCCGACCAG CAGAGCTTCT ACAAAGATGC TTGAAGATGA CTTGAAGCTG
    AGCAGTGATG AAGATGACCT TGAGCCCATG AAGACCTTGA CCACTCAGTG CACTGCCACT
    GAGCTCTACC AGGCTGTTGA AAAGGCAAAA CCTAAGAATA ATCCTGTGAA CCCACTCTTG
    GCCACGCCCC AACCCCCACC TGCAGCGCCC ACCAGTGGGG GATCCGGCAG CTCCAGTGAC
    TCAGAGAGCA GCTCAGAGTC TGACTCAGAC ACTGAAAGCA GCACCACTGA CAGCGAAGCC
    AATGAGGCGC CGCGAGTGAC CACTCCAGAG CCTGAGCCGC CCTCCACTAA CAAGTGGCAG
    TTGGACAAAT GGCTTAACAA AGTGACTTCT CAAAACAAAT CTTTTATTTG TGGCCAAAAT
    GAAACACCCA TGGAGACGAT TTCTGTGCCT CCTCCAGTCA TTCAGCCAAT GGAAGTCCAG
    GTCAAGGTGA AGGCAAACCC CAGCCAGGTC CTGCCTGAAC CCAAAGAGAG GCCTCTCCTT
    GGTCTTATCA GGGAGAAAGC CCGTCCACGA CCCACCCTGA AAACTCCAGA AACAAAGGCT
    CTGAAGCATA AGTTGTCCAC AACTGTTGAG ACAGCATCTC AAAGGACAAT TGGGAAAAAA
    CAGCCCAAAA GGGTCGAGAA GAACACCAGC GTTGAGGAAT TTACCTGGCC CAAACCAAAT
    ATCACCAGCA GCACTCCCAA AGAAAAGGAA AGCGGGGAGC TTCCTGACCT CCCTAGAGGG
    CGCAGCAAAG CCGTTGCCCA CAAGCCGGTT CCCAGGAAAG AACCGAGGGC CAGCATCCCC
    TTGGCTCCCG AGAAGAAAAA GTACCGAGGG CCTGGCAAGA CGGTTCCAAA GTCCCGGGAA
    TTCATTGAGA CCGATTCGTC TACATCTGAT TCCAACACGG ATCAGGAAGA GCCCCTGCAG
    ATCAAAGTCC TGCCTCCATG CATGGCTCCA GGGGGCAACA CGACCAAATC CAAGGAAGCC
    GGAGGGGCCA GCCTGACCCT CAGCAGCTTT CTCAGCAGTG GCAATGGCAA CAACCCACCA
    GTCCCGAGTG AGGAACCTAC TCAGTCACCG GTCCCTGTCA CACAAACTGA GGTTCTATCT
    CCTGTCCGAG ATCAGGAGAA TCTGAAAAAT CTCTGGGTGA AGATTGACCT TGACCTACTG
    TCTCGAGTGC CTGGCCAGAA CTCACTCCAG GCAACGTCAG CCAAGCTAGA CCACAAGGAG
    ACAGCCTCAA AGCCCAAGCG GCAGACGGCT GCGGTGCCTG CAGAGAAAGC CGCCCCCAAG
    GGCAAGCGTA AGCACAAGCC AACAGAAGTT GCAGGGAAAA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CAGCCACCAT CTGCCTGCTG CCACCTTGCA TCTCGCCAGC TCCCCCCCAG
    AAGCCTCCCA GCACTAAAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAAGAAGAA
    AAGCTGTTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGTCG CAGGAACATC
    AGCGGCAATA ATGGTCCCTT CAGTCGAGAC AAAACCATTC CTTTGATTGG ACAGATCACC
    TCGACCAAAC CTAAGAGGAA TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATAGCTGTA
    AACGAGGGAG ACACTCCAAA AAAGGCAGCC CCTGGCACTG TCACCAACAC TGCTATCGCC
    TCTGCCACTG CCACTGCCAT TGTCACCAGC ACTGCCACAG CCACTGCCAC GGCCACTACC
    ACAACCACTA CCACAACCAT TTCCACCACC ACCTCCGCCA TCACCACTGG CCTCATGGAT
    AGCAGTCACC TGGAGATGAC ATCGTGGGCG GCCCTGCCCC TTCTGTCCAC CAGCACCACC
    AATGTCCGGA GACCCAAGCT CACTTTTGAT GACTCGGTTC ACAATGCTGA TTATTACATG
    CAAGAGGCTA AGAAGCTGAA GCACAAAGCT GATGCACTGT TCGAGAAATT TGGCAAAGCC
    GTGAATTACG CGGATGCAGC CCTGTCCTTC ACTGAATGTG GCAACGCGAT GGAGAGAGAC
    CCTCTGGAAG CCAAGTCTCC ATACACCATG TACTCCGAGA CTGTGGAACT CCTCAGGTAT
    GCCATGAGGC TGAAGAATTT TGCAAGCCCC CTGGCTTCAG ATGGGGACAA AAAGCTGGCT
    GTACTATGCT ACAGATGCCT GTCACTTCTC TACCTGAGGA TGTTCAAACT GAAGAAGGAC
    CATGCCATGA AGTACTCCAG ATCACTGATG GAATATTTTA AGCAAAATGC TTCAAAAGTC
    GCTCAGATAC CATCTCCATG GGTAGGCAAT GGAAAGAATA CTCCATCCCC AGTGTCTCTC
    AACAACGTCT CTCCCATCAA CACAATGGGG AACTGTAACA ACGGCCCAGT CACCATCCCA
    CAGCGCATTC ACCACATGGC CGCCAGCCAC GTCAACATCA CGAGCAATGT GTTACGGGGC
    TATGAACACT GGGACATGGC TGACAAACTG ACAAGAGAGA ACAAAGAATT CTTTGGCGAT
    CTGGACACGT TGATGGGGCC CCTGACGCAG CACAGCAGCA TGACCAATCT CGTCCGCTAC
    GTTCGCCAGG GACTGTGCTG GCTGCGCATC GACGCCCACT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227648.1
    CDS67..3954
    Translation

    Target ORF information:

    RefSeq Version XM_005227591.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227591.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAG
    ATGACCCATC CCTGGCCTAC TCCTCTCACT GCCATGCATA CTCCCGGAAA TTCTGAGCAG
    AGCACACTTT CCATCCCAGG ACAGGAATCC CAACATCTGA CTCCAGGATT TGCCTTACAA
    AAGTGGAGTG ACCCGACCAG CAGAGCTTCT ACAAAGATGC TTGAAGATGA CTTGAAGCTG
    AGCAGTGATG AAGATGACCT TGAGCCCATG AAGACCTTGA CCACTCAGTG CACTGCCACT
    GAGCTCTACC AGGCTGTTGA AAAGGCAAAA CCTAAGAATA ATCCTGTGAA CCCACTCTTG
    GCCACGCCCC AACCCCCACC TGCAGCGCCC ACCAGTGGGG GATCCGGCAG CTCCAGTGAC
    TCAGAGAGCA GCTCAGAGTC TGACTCAGAC ACTGAAAGCA GCACCACTGA CAGCGAAGCC
    AATGAGGCGC CGCGAGTGAC CACTCCAGAG CCTGAGCCGC CCTCCACTAA CAAGTGGCAG
    TTGGACAAAT GGCTTAACAA AGTGACTTCT CAAAACAAAT CTTTTATTTG TGGCCAAAAT
    GAAACACCCA TGGAGACGAT TTCTGTGCCT CCTCCAGTCA TTCAGCCAAT GGAAGTCCAG
    GTCAAGGTGA AGGCAAACCC CAGCCAGGTC CTGCCTGAAC CCAAAGAGAG GCCTCTCCTT
    GGTCTTATCA GGGAGAAAGC CCGTCCACGA CCCACCCTGA AAACTCCAGA AACAAAGGCT
    CTGAAGCATA AGTTGTCCAC AACTGTTGAG ACAGCATCTC AAAGGACAAT TGGGAAAAAA
    CAGCCCAAAA GGGTCGAGAA GAACACCAGC GTTGAGGAAT TTACCTGGCC CAAACCAAAT
    ATCACCAGCA GCACTCCCAA AGAAAAGGAA AGCGGGGAGC TTCCTGACCT CCCTAGAGGG
    CGCAGCAAAG CCGTTGCCCA CAAGCCGGTT CCCAGGAAAG AACCGAGGGC CAGCATCCCC
    TTGGCTCCCG AGAAGAAAAA GTACCGAGGG CCTGGCAAGA CGGTTCCAAA GTCCCGGGAA
    TTCATTGAGA CCGATTCGTC TACATCTGAT TCCAACACGG ATCAGGAAGA GCCCCTGCAG
    ATCAAAGTCC TGCCTCCATG CATGGCTCCA GGGGGCAACA CGACCAAATC CAAGGAAGCC
    GGAGGGGCCA GCCTGACCCT CAGCAGCTTT CTCAGCAGTG GCAATGGCAA CAACCCACCA
    GTCCCGAGTG AGGAACCTAC TCAGTCACCG GTCCCTGTCA CACAAACTGA GGTTCTATCT
    CCTGTCCGAG ATCAGGAGAA TCTGAAAAAT CTCTGGGTGA AGATTGACCT TGACCTACTG
    TCTCGAGTGC CTGGCCAGAA CTCACTCCAG GCAACGTCAG CCAAGCTAGA CCACAAGGAG
    ACAGCCTCAA AGCCCAAGCG GCAGACGGCT GCGGTGCCTG CAGAGAAAGC CGCCCCCAAG
    GGCAAGCGTA AGCACAAGCC AACAGAAGTT GCAGGGAAAA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CAGCCACCAT CTGCCTGCTG CCACCTTGCA TCTCGCCAGC TCCCCCCCAG
    AAGCCTCCCA GCACTAAAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAAGAAGAA
    AAGCTGTTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGTCG CAGGAACATC
    AGCGGCAATA ATGGTCCCTT CAGTCGAGAC AAAACCATTC CTTTGATTGG ACAGATCACC
    TCGACCAAAC CTAAGAGGAA TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATAGCTGTA
    AACGAGGGAG ACACTCCAAA AAAGGCAGCC CCTGGCACTG TCACCAACAC TGCTATCGCC
    TCTGCCACTG CCACTGCCAT TGTCACCAGC ACTGCCACAG CCACTGCCAC GGCCACTACC
    ACAACCACTA CCACAACCAT TTCCACCACC ACCTCCGCCA TCACCACTGG CCTCATGGAT
    AGCAGTCACC TGGAGATGAC ATCGTGGGCG GCCCTGCCCC TTCTGTCCAC CAGCACCACC
    AATGTCCGGA GACCCAAGCT CACTTTTGAT GACTCGGTTC ACAATGCTGA TTATTACATG
    CAAGAGGCTA AGAAGCTGAA GCACAAAGCT GATGCACTGT TCGAGAAATT TGGCAAAGCC
    GTGAATTACG CGGATGCAGC CCTGTCCTTC ACTGAATGTG GCAACGCGAT GGAGAGAGAC
    CCTCTGGAAG CCAAGTCTCC ATACACCATG TACTCCGAGA CTGTGGAACT CCTCAGGTAT
    GCCATGAGGC TGAAGAATTT TGCAAGCCCC CTGGCTTCAG ATGGGGACAA AAAGCTGGCT
    GTACTATGCT ACAGATGCCT GTCACTTCTC TACCTGAGGA TGTTCAAACT GAAGAAGGAC
    CATGCCATGA AGTACTCCAG ATCACTGATG GAATATTTTA AGCAAAATGC TTCAAAAGTC
    GCTCAGATAC CATCTCCATG GGTAGGCAAT GGAAAGAATA CTCCATCCCC AGTGTCTCTC
    AACAACGTCT CTCCCATCAA CACAATGGGG AACTGTAACA ACGGCCCAGT CACCATCCCA
    CAGCGCATTC ACCACATGGC CGCCAGCCAC GTCAACATCA CGAGCAATGT GTTACGGGGC
    TATGAACACT GGGACATGGC TGACAAACTG ACAAGAGAGA ACAAAGAATT CTTTGGCGAT
    CTGGACACGT TGATGGGGCC CCTGACGCAG CACAGCAGCA TGACCAATCT CGTCCGCTAC
    GTTCGCCAGG GACTGTGCTG GCTGCGCATC GACGCCCACT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35222
    Clone ID Related Accession (Same CDS sequence) XM_005227592.4 , XM_005227592.3
    Accession Version XM_005227592.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227592.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAA
    TCCCAACATC TGACTCCAGG ATTTGCCTTA CAAAAGTGGA GTGACCCGAC CAGCAGAGCT
    TCTACAAAGA TGCTTGAAGA TGACTTGAAG CTGAGCAGTG ATGAAGATGA CCTTGAGCCC
    ATGAAGACCT TGACCACTCA GTGCACTGCC ACTGAGCTCT ACCAGGCTGT TGAAAAGGCA
    AAACCTAAGA ATAATCCTGT GAACCCACTC TTGGCCACGC CCCAACCCCC ACCTGCAGCG
    CCCACCAGTG GGGGATCCGG CAGCTCCAGT GACTCAGAGA GCAGCTCAGA GTCTGACTCA
    GACACTGAAA GCAGCACCAC TGACAGCGAA GCCAATGAGG CGCCGCGAGT GACCACTCCA
    GAGCCTGAGC CGCCCTCCAC TAACAAGTGG CAGTTGGACA AATGGCTTAA CAAAGTGACT
    TCTCAAAACA AATCTTTTAT TTGTGGCCAA AATGAAACAC CCATGGAGAC GATTTCTGTG
    CCTCCTCCAG TCATTCAGCC AATGGAAGTC CAGGTCAAGG TGAAGGCAAA CCCCAGCCAG
    GTCCTGCCTG AACCCAAAGA GAGGCCTCTC CTTGGTCTTA TCAGGGAGAA AGCCCGTCCA
    CGACCCACCC TGAAAACTCC AGAAACAAAG GCTCTGAAGC ATAAGTTGTC CACAACTGTT
    GAGACAGCAT CTCAAAGGAC AATTGGGAAA AAACAGCCCA AAAGGGTCGA GAAGAACACC
    AGCGTTGAGG AATTTACCTG GCCCAAACCA AATATCACCA GCAGCACTCC CAAAGAAAAG
    GAAAGCGGGG AGCTTCCTGA CCTCCCTAGA GGGCGCAGCA AAGCCGTTGC CCACAAGCCG
    GTTCCCAGGA AAGAACCGAG GGCCAGCATC CCCTTGGCTC CCGAGAAGAA AAAGTACCGA
    GGGCCTGGCA AGACGGTTCC AAAGTCCCGG GAATTCATTG AGACCGATTC GTCTACATCT
    GATTCCAACA CGGATCAGGA AGAGCCCCTG CAGATCAAAG TCCTGCCTCC ATGCATGGCT
    CCAGGGGGCA ACACGACCAA ATCCAAGGAA GCCGGAGGGG CCAGCCTGAC CCTCAGCAGC
    TTTCTCAGCA GTGGCAATGG CAACAACCCA CCAGTCCCGA GTGAGGAACC TACTCAGTCA
    CCGGTCCCTG TCACACAAAC TGAGGTTCTA TCTCCTGTCC GAGATCAGGA GAATCTGAAA
    AATCTCTGGG TGAAGATTGA CCTTGACCTA CTGTCTCGAG TGCCTGGCCA GAACTCACTC
    CAGGCAACGT CAGCCAAGCT AGACCACAAG GAGACAGCCT CAAAGCCCAA GCGGCAGACG
    GCTGCGGTGC CTGCAGAGAA AGCCGCCCCC AAGGGCAAGC GTAAGCACAA GCCAACAGAA
    GTTGCAGGGA AAATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCAGCCAC CATCTGCCTG
    CTGCCACCTT GCATCTCGCC AGCTCCCCCC CAGAAGCCTC CCAGCACTAA AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAAGAA GAAAAGCTGT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG TCGCAGGAAC ATCAGCGGCA ATAATGGTCC CTTCAGTCGA
    GACAAAACCA TTCCTTTGAT TGGACAGATC ACCTCGACCA AACCTAAGAG GAATGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATAGCT GTAAACGAGG GAGACACTCC AAAAAAGGCA
    GCCCCTGGCA CTGTCACCAA CACTGCTATC GCCTCTGCCA CTGCCACTGC CATTGTCACC
    AGCACTGCCA CAGCCACTGC CACGGCCACT ACCACAACCA CTACCACAAC CATTTCCACC
    ACCACCTCCG CCATCACCAC TGGCCTCATG GATAGCAGTC ACCTGGAGAT GACATCGTGG
    GCGGCCCTGC CCCTTCTGTC CACCAGCACC ACCAATGTCC GGAGACCCAA GCTCACTTTT
    GATGACTCGG TTCACAATGC TGATTATTAC ATGCAAGAGG CTAAGAAGCT GAAGCACAAA
    GCTGATGCAC TGTTCGAGAA ATTTGGCAAA GCCGTGAATT ACGCGGATGC AGCCCTGTCC
    TTCACTGAAT GTGGCAACGC GATGGAGAGA GACCCTCTGG AAGCCAAGTC TCCATACACC
    ATGTACTCCG AGACTGTGGA ACTCCTCAGG TATGCCATGA GGCTGAAGAA TTTTGCAAGC
    CCCCTGGCTT CAGATGGGGA CAAAAAGCTG GCTGTACTAT GCTACAGATG CCTGTCACTT
    CTCTACCTGA GGATGTTCAA ACTGAAGAAG GACCATGCCA TGAAGTACTC CAGATCACTG
    ATGGAATATT TTAAGCAAAA TGCTTCAAAA GTCGCTCAGA TACCATCTCC ATGGGTAGGC
    AATGGAAAGA ATACTCCATC CCCAGTGTCT CTCAACAACG TCTCTCCCAT CAACACAATG
    GGGAACTGTA ACAACGGCCC AGTCACCATC CCACAGCGCA TTCACCACAT GGCCGCCAGC
    CACGTCAACA TCACGAGCAA TGTGTTACGG GGCTATGAAC ACTGGGACAT GGCTGACAAA
    CTGACAAGAG AGAACAAAGA ATTCTTTGGC GATCTGGACA CGTTGATGGG GCCCCTGACG
    CAGCACAGCA GCATGACCAA TCTCGTCCGC TACGTTCGCC AGGGACTGTG CTGGCTGCGC
    ATCGACGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227649.1
    CDS276..4076
    Translation

    Target ORF information:

    RefSeq Version XM_005227592.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227592.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAA
    TCCCAACATC TGACTCCAGG ATTTGCCTTA CAAAAGTGGA GTGACCCGAC CAGCAGAGCT
    TCTACAAAGA TGCTTGAAGA TGACTTGAAG CTGAGCAGTG ATGAAGATGA CCTTGAGCCC
    ATGAAGACCT TGACCACTCA GTGCACTGCC ACTGAGCTCT ACCAGGCTGT TGAAAAGGCA
    AAACCTAAGA ATAATCCTGT GAACCCACTC TTGGCCACGC CCCAACCCCC ACCTGCAGCG
    CCCACCAGTG GGGGATCCGG CAGCTCCAGT GACTCAGAGA GCAGCTCAGA GTCTGACTCA
    GACACTGAAA GCAGCACCAC TGACAGCGAA GCCAATGAGG CGCCGCGAGT GACCACTCCA
    GAGCCTGAGC CGCCCTCCAC TAACAAGTGG CAGTTGGACA AATGGCTTAA CAAAGTGACT
    TCTCAAAACA AATCTTTTAT TTGTGGCCAA AATGAAACAC CCATGGAGAC GATTTCTGTG
    CCTCCTCCAG TCATTCAGCC AATGGAAGTC CAGGTCAAGG TGAAGGCAAA CCCCAGCCAG
    GTCCTGCCTG AACCCAAAGA GAGGCCTCTC CTTGGTCTTA TCAGGGAGAA AGCCCGTCCA
    CGACCCACCC TGAAAACTCC AGAAACAAAG GCTCTGAAGC ATAAGTTGTC CACAACTGTT
    GAGACAGCAT CTCAAAGGAC AATTGGGAAA AAACAGCCCA AAAGGGTCGA GAAGAACACC
    AGCGTTGAGG AATTTACCTG GCCCAAACCA AATATCACCA GCAGCACTCC CAAAGAAAAG
    GAAAGCGGGG AGCTTCCTGA CCTCCCTAGA GGGCGCAGCA AAGCCGTTGC CCACAAGCCG
    GTTCCCAGGA AAGAACCGAG GGCCAGCATC CCCTTGGCTC CCGAGAAGAA AAAGTACCGA
    GGGCCTGGCA AGACGGTTCC AAAGTCCCGG GAATTCATTG AGACCGATTC GTCTACATCT
    GATTCCAACA CGGATCAGGA AGAGCCCCTG CAGATCAAAG TCCTGCCTCC ATGCATGGCT
    CCAGGGGGCA ACACGACCAA ATCCAAGGAA GCCGGAGGGG CCAGCCTGAC CCTCAGCAGC
    TTTCTCAGCA GTGGCAATGG CAACAACCCA CCAGTCCCGA GTGAGGAACC TACTCAGTCA
    CCGGTCCCTG TCACACAAAC TGAGGTTCTA TCTCCTGTCC GAGATCAGGA GAATCTGAAA
    AATCTCTGGG TGAAGATTGA CCTTGACCTA CTGTCTCGAG TGCCTGGCCA GAACTCACTC
    CAGGCAACGT CAGCCAAGCT AGACCACAAG GAGACAGCCT CAAAGCCCAA GCGGCAGACG
    GCTGCGGTGC CTGCAGAGAA AGCCGCCCCC AAGGGCAAGC GTAAGCACAA GCCAACAGAA
    GTTGCAGGGA AAATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCAGCCAC CATCTGCCTG
    CTGCCACCTT GCATCTCGCC AGCTCCCCCC CAGAAGCCTC CCAGCACTAA AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAAGAA GAAAAGCTGT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG TCGCAGGAAC ATCAGCGGCA ATAATGGTCC CTTCAGTCGA
    GACAAAACCA TTCCTTTGAT TGGACAGATC ACCTCGACCA AACCTAAGAG GAATGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATAGCT GTAAACGAGG GAGACACTCC AAAAAAGGCA
    GCCCCTGGCA CTGTCACCAA CACTGCTATC GCCTCTGCCA CTGCCACTGC CATTGTCACC
    AGCACTGCCA CAGCCACTGC CACGGCCACT ACCACAACCA CTACCACAAC CATTTCCACC
    ACCACCTCCG CCATCACCAC TGGCCTCATG GATAGCAGTC ACCTGGAGAT GACATCGTGG
    GCGGCCCTGC CCCTTCTGTC CACCAGCACC ACCAATGTCC GGAGACCCAA GCTCACTTTT
    GATGACTCGG TTCACAATGC TGATTATTAC ATGCAAGAGG CTAAGAAGCT GAAGCACAAA
    GCTGATGCAC TGTTCGAGAA ATTTGGCAAA GCCGTGAATT ACGCGGATGC AGCCCTGTCC
    TTCACTGAAT GTGGCAACGC GATGGAGAGA GACCCTCTGG AAGCCAAGTC TCCATACACC
    ATGTACTCCG AGACTGTGGA ACTCCTCAGG TATGCCATGA GGCTGAAGAA TTTTGCAAGC
    CCCCTGGCTT CAGATGGGGA CAAAAAGCTG GCTGTACTAT GCTACAGATG CCTGTCACTT
    CTCTACCTGA GGATGTTCAA ACTGAAGAAG GACCATGCCA TGAAGTACTC CAGATCACTG
    ATGGAATATT TTAAGCAAAA TGCTTCAAAA GTCGCTCAGA TACCATCTCC ATGGGTAGGC
    AATGGAAAGA ATACTCCATC CCCAGTGTCT CTCAACAACG TCTCTCCCAT CAACACAATG
    GGGAACTGTA ACAACGGCCC AGTCACCATC CCACAGCGCA TTCACCACAT GGCCGCCAGC
    CACGTCAACA TCACGAGCAA TGTGTTACGG GGCTATGAAC ACTGGGACAT GGCTGACAAA
    CTGACAAGAG AGAACAAAGA ATTCTTTGGC GATCTGGACA CGTTGATGGG GCCCCTGACG
    CAGCACAGCA GCATGACCAA TCTCGTCCGC TACGTTCGCC AGGGACTGTG CTGGCTGCGC
    ATCGACGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35222
    Clone ID Related Accession (Same CDS sequence) XM_005227592.4 , XM_005227592.3
    Accession Version XM_005227592.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104664.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAA
    TCCCAACATC TGACTCCAGG ATTTGCCTTA CAAAAGTGGA GTGACCCGAC CAGCAGAGCT
    TCTACAAAGA TGCTTGAAGA TGACTTGAAG CTGAGCAGTG ATGAAGATGA CCTTGAGCCC
    ATGAAGACCT TGACCACTCA GTGCACTGCC ACTGAGCTCT ACCAGGCTGT TGAAAAGGCA
    AAACCTAAGA ATAATCCTGT GAACCCACTC TTGGCCACGC CCCAACCCCC ACCTGCAGCG
    CCCACCAGTG GGGGATCCGG CAGCTCCAGT GACTCAGAGA GCAGCTCAGA GTCTGACTCA
    GACACTGAAA GCAGCACCAC TGACAGCGAA GCCAATGAGG CGCCGCGAGT GACCACTCCA
    GAGCCTGAGC CGCCCTCCAC TAACAAGTGG CAGTTGGACA AATGGCTTAA CAAAGTGACT
    TCTCAAAACA AATCTTTTAT TTGTGGCCAA AATGAAACAC CCATGGAGAC GATTTCTGTG
    CCTCCTCCAG TCATTCAGCC AATGGAAGTC CAGGTCAAGG TGAAGGCAAA CCCCAGCCAG
    GTCCTGCCTG AACCCAAAGA GAGGCCTCTC CTTGGTCTTA TCAGGGAGAA AGCCCGTCCA
    CGACCCACCC TGAAAACTCC AGAAACAAAG GCTCTGAAGC ATAAGTTGTC CACAACTGTT
    GAGACAGCAT CTCAAAGGAC AATTGGGAAA AAACAGCCCA AAAGGGTCGA GAAGAACACC
    AGCGTTGAGG AATTTACCTG GCCCAAACCA AATATCACCA GCAGCACTCC CAAAGAAAAG
    GAAAGCGGGG AGCTTCCTGA CCTCCCTAGA GGGCGCAGCA AAGCCGTTGC CCACAAGCCG
    GTTCCCAGGA AAGAACCGAG GGCCAGCATC CCCTTGGCTC CCGAGAAGAA AAAGTACCGA
    GGGCCTGGCA AGACGGTTCC AAAGTCCCGG GAATTCATTG AGACCGATTC GTCTACATCT
    GATTCCAACA CGGATCAGGA AGAGCCCCTG CAGATCAAAG TCCTGCCTCC ATGCATGGCT
    CCAGGGGGCA ACACGACCAA ATCCAAGGAA GCCGGAGGGG CCAGCCTGAC CCTCAGCAGC
    TTTCTCAGCA GTGGCAATGG CAACAACCCA CCAGTCCCGA GTGAGGAACC TACTCAGTCA
    CCGGTCCCTG TCACACAAAC TGAGGTTCTA TCTCCTGTCC GAGATCAGGA GAATCTGAAA
    AATCTCTGGG TGAAGATTGA CCTTGACCTA CTGTCTCGAG TGCCTGGCCA GAACTCACTC
    CAGGCAACGT CAGCCAAGCT AGACCACAAG GAGACAGCCT CAAAGCCCAA GCGGCAGACG
    GCTGCGGTGC CTGCAGAGAA AGCCGCCCCC AAGGGCAAGC GTAAGCACAA GCCAACAGAA
    GTTGCAGGGA AAATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCAGCCAC CATCTGCCTG
    CTGCCACCTT GCATCTCGCC AGCTCCCCCC CAGAAGCCTC CCAGCACTAA AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAAGAA GAAAAGCTGT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG TCGCAGGAAC ATCAGCGGCA ATAATGGTCC CTTCAGTCGA
    GACAAAACCA TTCCTTTGAT TGGACAGATC ACCTCGACCA AACCTAAGAG GAATGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATAGCT GTAAACGAGG GAGACACTCC AAAAAAGGCA
    GCCCCTGGCA CTGTCACCAA CACTGCTATC GCCTCTGCCA CTGCCACTGC CATTGTCACC
    AGCACTGCCA CAGCCACTGC CACGGCCACT ACCACAACCA CTACCACAAC CATTTCCACC
    ACCACCTCCG CCATCACCAC TGGCCTCATG GATAGCAGTC ACCTGGAGAT GACATCGTGG
    GCGGCCCTGC CCCTTCTGTC CACCAGCACC ACCAATGTCC GGAGACCCAA GCTCACTTTT
    GATGACTCGG TTCACAATGC TGATTATTAC ATGCAAGAGG CTAAGAAGCT GAAGCACAAA
    GCTGATGCAC TGTTCGAGAA ATTTGGCAAA GCCGTGAATT ACGCGGATGC AGCCCTGTCC
    TTCACTGAAT GTGGCAACGC GATGGAGAGA GACCCTCTGG AAGCCAAGTC TCCATACACC
    ATGTACTCCG AGACTGTGGA ACTCCTCAGG TATGCCATGA GGCTGAAGAA TTTTGCAAGC
    CCCCTGGCTT CAGATGGGGA CAAAAAGCTG GCTGTACTAT GCTACAGATG CCTGTCACTT
    CTCTACCTGA GGATGTTCAA ACTGAAGAAG GACCATGCCA TGAAGTACTC CAGATCACTG
    ATGGAATATT TTAAGCAAAA TGCTTCAAAA GTCGCTCAGA TACCATCTCC ATGGGTAGGC
    AATGGAAAGA ATACTCCATC CCCAGTGTCT CTCAACAACG TCTCTCCCAT CAACACAATG
    GGGAACTGTA ACAACGGCCC AGTCACCATC CCACAGCGCA TTCACCACAT GGCCGCCAGC
    CACGTCAACA TCACGAGCAA TGTGTTACGG GGCTATGAAC ACTGGGACAT GGCTGACAAA
    CTGACAAGAG AGAACAAAGA ATTCTTTGGC GATCTGGACA CGTTGATGGG GCCCCTGACG
    CAGCACAGCA GCATGACCAA TCTCGTCCGC TACGTTCGCC AGGGACTGTG CTGGCTGCGC
    ATCGACGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227649.1
    CDS67..3867
    Translation

    Target ORF information:

    RefSeq Version XM_005227592.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227592.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGGGACTGG GACCTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAATG CACTTAAGAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CCTACAAGAC AAACAAAGGT
    GATGCACTCG CGAACCGAGT CCAGAACACG CTGGGAAATT ACGATGAAAT GAAGGATTTG
    CTAACTAACC ATTCTAATCA GAATCATCTA GTGGGAATCC CAAAGAATTC CATGCCCCAG
    ACTCCCACCA ACAAAAATGA ACCCAGCTTT TTTCCAGAAC AAAAGCACCG AATGGTCCCA
    GTGCACCAGC AGGACAACAC TCACTCCTCG GCGCCAATGC CTCCACCTTC TGTCGTGATA
    CTGAATTCAA CTCTAATACA CAGCAACCGG AAGTCAAAAC CTGACTGGTC ACGAGATAGT
    CAGAACACTA GCATTGTAGC AGCCAGCCAA GTCACTAGTC AGCCCAACAA GATGCAGCCG
    CCAACCCCAG ACCAGCCCCC AGCCAGATTG GAAGACTTCT TTGTCTACCC AGCTGAACAG
    CCTCAAGTTG GAGCAGTTGA AGAATCAAAC CCACTGGCAA AGGAAGACAA TACTCTCAAG
    TCCAGTGGAG GGGATACTTT CAAAGAAATC TATCAGTCCA ACTCACCAGA AGCAGCGGAA
    TTTACAGTGC AAGCGCCTGG ATCTCCCCTA GTTGCCTCGT CTCTATTAGC TCCCAGCAGT
    GGCCTTTCGG TTCAAAACTT CCCACCAGGG CTTTACTGCA AAACAAGCAT GGGGCAGCAA
    AAACCAACCG CCTATGTCCG ACCCATGGAT GGTCAGGACC AGGCCCCGGA CATCTCTCCC
    ACACTGAAAC CTTCGATTGA ATTTGACAAC AGCTTTGGGA ATCTGTCGTT TGGATCACTC
    TTGGATGGCA AACCCAGTGC ACCCAGTTCA AAGACTAAAC AGCCAAAGTT CACGATTCTT
    CAAACAAGTG AAGTAAGCCT TCCCAGTGAC CCAAGCTGTG TTGAAGAAAT CTTGCGGGAA
    TCCCAACATC TGACTCCAGG ATTTGCCTTA CAAAAGTGGA GTGACCCGAC CAGCAGAGCT
    TCTACAAAGA TGCTTGAAGA TGACTTGAAG CTGAGCAGTG ATGAAGATGA CCTTGAGCCC
    ATGAAGACCT TGACCACTCA GTGCACTGCC ACTGAGCTCT ACCAGGCTGT TGAAAAGGCA
    AAACCTAAGA ATAATCCTGT GAACCCACTC TTGGCCACGC CCCAACCCCC ACCTGCAGCG
    CCCACCAGTG GGGGATCCGG CAGCTCCAGT GACTCAGAGA GCAGCTCAGA GTCTGACTCA
    GACACTGAAA GCAGCACCAC TGACAGCGAA GCCAATGAGG CGCCGCGAGT GACCACTCCA
    GAGCCTGAGC CGCCCTCCAC TAACAAGTGG CAGTTGGACA AATGGCTTAA CAAAGTGACT
    TCTCAAAACA AATCTTTTAT TTGTGGCCAA AATGAAACAC CCATGGAGAC GATTTCTGTG
    CCTCCTCCAG TCATTCAGCC AATGGAAGTC CAGGTCAAGG TGAAGGCAAA CCCCAGCCAG
    GTCCTGCCTG AACCCAAAGA GAGGCCTCTC CTTGGTCTTA TCAGGGAGAA AGCCCGTCCA
    CGACCCACCC TGAAAACTCC AGAAACAAAG GCTCTGAAGC ATAAGTTGTC CACAACTGTT
    GAGACAGCAT CTCAAAGGAC AATTGGGAAA AAACAGCCCA AAAGGGTCGA GAAGAACACC
    AGCGTTGAGG AATTTACCTG GCCCAAACCA AATATCACCA GCAGCACTCC CAAAGAAAAG
    GAAAGCGGGG AGCTTCCTGA CCTCCCTAGA GGGCGCAGCA AAGCCGTTGC CCACAAGCCG
    GTTCCCAGGA AAGAACCGAG GGCCAGCATC CCCTTGGCTC CCGAGAAGAA AAAGTACCGA
    GGGCCTGGCA AGACGGTTCC AAAGTCCCGG GAATTCATTG AGACCGATTC GTCTACATCT
    GATTCCAACA CGGATCAGGA AGAGCCCCTG CAGATCAAAG TCCTGCCTCC ATGCATGGCT
    CCAGGGGGCA ACACGACCAA ATCCAAGGAA GCCGGAGGGG CCAGCCTGAC CCTCAGCAGC
    TTTCTCAGCA GTGGCAATGG CAACAACCCA CCAGTCCCGA GTGAGGAACC TACTCAGTCA
    CCGGTCCCTG TCACACAAAC TGAGGTTCTA TCTCCTGTCC GAGATCAGGA GAATCTGAAA
    AATCTCTGGG TGAAGATTGA CCTTGACCTA CTGTCTCGAG TGCCTGGCCA GAACTCACTC
    CAGGCAACGT CAGCCAAGCT AGACCACAAG GAGACAGCCT CAAAGCCCAA GCGGCAGACG
    GCTGCGGTGC CTGCAGAGAA AGCCGCCCCC AAGGGCAAGC GTAAGCACAA GCCAACAGAA
    GTTGCAGGGA AAATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCAGCCAC CATCTGCCTG
    CTGCCACCTT GCATCTCGCC AGCTCCCCCC CAGAAGCCTC CCAGCACTAA AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAAGAA GAAAAGCTGT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG TCGCAGGAAC ATCAGCGGCA ATAATGGTCC CTTCAGTCGA
    GACAAAACCA TTCCTTTGAT TGGACAGATC ACCTCGACCA AACCTAAGAG GAATGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATAGCT GTAAACGAGG GAGACACTCC AAAAAAGGCA
    GCCCCTGGCA CTGTCACCAA CACTGCTATC GCCTCTGCCA CTGCCACTGC CATTGTCACC
    AGCACTGCCA CAGCCACTGC CACGGCCACT ACCACAACCA CTACCACAAC CATTTCCACC
    ACCACCTCCG CCATCACCAC TGGCCTCATG GATAGCAGTC ACCTGGAGAT GACATCGTGG
    GCGGCCCTGC CCCTTCTGTC CACCAGCACC ACCAATGTCC GGAGACCCAA GCTCACTTTT
    GATGACTCGG TTCACAATGC TGATTATTAC ATGCAAGAGG CTAAGAAGCT GAAGCACAAA
    GCTGATGCAC TGTTCGAGAA ATTTGGCAAA GCCGTGAATT ACGCGGATGC AGCCCTGTCC
    TTCACTGAAT GTGGCAACGC GATGGAGAGA GACCCTCTGG AAGCCAAGTC TCCATACACC
    ATGTACTCCG AGACTGTGGA ACTCCTCAGG TATGCCATGA GGCTGAAGAA TTTTGCAAGC
    CCCCTGGCTT CAGATGGGGA CAAAAAGCTG GCTGTACTAT GCTACAGATG CCTGTCACTT
    CTCTACCTGA GGATGTTCAA ACTGAAGAAG GACCATGCCA TGAAGTACTC CAGATCACTG
    ATGGAATATT TTAAGCAAAA TGCTTCAAAA GTCGCTCAGA TACCATCTCC ATGGGTAGGC
    AATGGAAAGA ATACTCCATC CCCAGTGTCT CTCAACAACG TCTCTCCCAT CAACACAATG
    GGGAACTGTA ACAACGGCCC AGTCACCATC CCACAGCGCA TTCACCACAT GGCCGCCAGC
    CACGTCAACA TCACGAGCAA TGTGTTACGG GGCTATGAAC ACTGGGACAT GGCTGACAAA
    CTGACAAGAG AGAACAAAGA ATTCTTTGGC GATCTGGACA CGTTGATGGG GCCCCTGACG
    CAGCACAGCA GCATGACCAA TCTCGTCCGC TACGTTCGCC AGGGACTGTG CTGGCTGCGC
    ATCGACGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.