ITSN2 cDNA ORF clone, Bos taurus(cattle)

The following ITSN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220886 XM_024999513.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220886 XM_024999512.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220887 XM_024999514.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220885 XM_024999510.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa220885 XM_024999509.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa91949 XM_015465428.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91949 XM_015473567.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91949 XM_024999511.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa50445 XM_015465423.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810287.3
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810288.2
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_015465426.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810285.3
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810286.3
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_015465424.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_015465425.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50446 XM_010810289.3
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50446 XM_015465427.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50447 XM_010810290.2
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa50447 XM_015465429.1
Latest version!
Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OBa50446 XM_010810289.2 Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810286.2 Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810287.2 Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50445 XM_010810285.2 Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220886
    Clone ID Related Accession (Same CDS sequence) XM_024999513.1 , XM_024999512.1
    Accession Version XM_024999513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855281.1
    CDS266..3712
    Translation

    Target ORF information:

    RefSeq Version XM_024999513.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220886
    Clone ID Related Accession (Same CDS sequence) XM_024999513.1 , XM_024999512.1
    Accession Version XM_024999512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855280.1
    CDS169..3615
    Translation

    Target ORF information:

    RefSeq Version XM_024999512.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220887
    Clone ID Related Accession (Same CDS sequence) XM_024999514.1
    Accession Version XM_024999514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855282.1
    CDS222..3626
    Translation

    Target ORF information:

    RefSeq Version XM_024999514.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220885
    Clone ID Related Accession (Same CDS sequence) XM_024999509.1 , XM_024999510.1
    Accession Version XM_024999509.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA
    ACACTGGACA CCATGCAGCC GGTGGAAAGG AAGAGGCAGG GCTACATCCA CGAGCTGATT
    GAGACGGAGG AGCGCTACGT GGACGACCTG CAGCTCGTCG TGGAGGTTTT TCAGAAACGA
    ATAACAGAGC CAGGTTTTCT CGCTGAAGGA GAGATGGCCC TGATCTTTGT TAACTGGAAG
    GAGCTCATCA TGGCTAACAC GAAGCTGCTG AAGGCGCTGC GGGTGCGGAA GAAGACCGGG
    GGTGAGAAGA TGCCCGTGCA CATGATCGGG GACATCCTGG CCGCTGAGCT GTCCCACATG
    CAGGCCTACA TCCGCTTCTG CAGCTGTCAG CTTAATGGAG CAGCCCTGTT ACAGCAGAAG
    ACAGACGAAC ACGCAGACTT CAAAGAATTT TTAAAGAAGT TGGCATCTGA CCCTCGGTGT
    AAAGGGATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGGATCAC CCGCTACCCA
    CTCCTCATCA GAAGCATTCT GGAGAACACC CCAGAGAATC ATGTAGACCA CTCCTCTCTG
    AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC TGCTCTCAAG TGAATGAAGG AGTTCGGGAG
    AAGGAAAATT CAGACCGACT GGAGTGGATC CAGACGCACG TGCAGTGCGA TGGGCTTGCG
    GAGCAACTTG TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT CCTGCACAGT
    GGAAAGCTGT ACAAGACCAA GAGCAACAAG GAGCTACACG GGTTCCTCTT CAACGACTTC
    CTGCTTCTCA CCTACATGGT CAAGCAGTTC GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC
    AGCTCAAAGT CCAGTGCTCA GTTCAAGATG TATAAGACGC CCATTTTCCT GAATGAAGCC
    TTGGTAAAAC TGCCCACAGA CCCTTCCAGC GACGAGCCTG TCTTCCACAT CTCTCACATT
    GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACGGCCTG GGTCCAGAAG
    ATCAAGGCCG CGTCTGAGCA GTACATCGAC ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT
    CAAGCTCGCT CTCAGAAGTC TTCAGGCATC GGGCGTCTGA TGGTGCATGT CATTGAAGCT
    ACAGAACTGA AAGCCTGTAA ACCAAATGGA AAGAGCAATC CGTACTGTGA AATCAGCATG
    GGCTCCCAGA GCTACACCAC CAGAACCCTC CAGGACACAC TAAATCCCAA ATGGAATTTT
    AACTGCCAGT TCTTCATTAA GGACCTCTAT CAGGACGTGC TGTGTCTTAC CATGTTCGAC
    AGAGATCAGT TTTCACCAGA CGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT
    CGAACAGAAC AAGAAAGCAA AGGCCCTACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC
    ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855277.1
    CDS89..5008
    Translation

    Target ORF information:

    RefSeq Version XM_024999509.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999509.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA
    ACACTGGACA CCATGCAGCC GGTGGAAAGG AAGAGGCAGG GCTACATCCA CGAGCTGATT
    GAGACGGAGG AGCGCTACGT GGACGACCTG CAGCTCGTCG TGGAGGTTTT TCAGAAACGA
    ATAACAGAGC CAGGTTTTCT CGCTGAAGGA GAGATGGCCC TGATCTTTGT TAACTGGAAG
    GAGCTCATCA TGGCTAACAC GAAGCTGCTG AAGGCGCTGC GGGTGCGGAA GAAGACCGGG
    GGTGAGAAGA TGCCCGTGCA CATGATCGGG GACATCCTGG CCGCTGAGCT GTCCCACATG
    CAGGCCTACA TCCGCTTCTG CAGCTGTCAG CTTAATGGAG CAGCCCTGTT ACAGCAGAAG
    ACAGACGAAC ACGCAGACTT CAAAGAATTT TTAAAGAAGT TGGCATCTGA CCCTCGGTGT
    AAAGGGATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGGATCAC CCGCTACCCA
    CTCCTCATCA GAAGCATTCT GGAGAACACC CCAGAGAATC ATGTAGACCA CTCCTCTCTG
    AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC TGCTCTCAAG TGAATGAAGG AGTTCGGGAG
    AAGGAAAATT CAGACCGACT GGAGTGGATC CAGACGCACG TGCAGTGCGA TGGGCTTGCG
    GAGCAACTTG TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT CCTGCACAGT
    GGAAAGCTGT ACAAGACCAA GAGCAACAAG GAGCTACACG GGTTCCTCTT CAACGACTTC
    CTGCTTCTCA CCTACATGGT CAAGCAGTTC GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC
    AGCTCAAAGT CCAGTGCTCA GTTCAAGATG TATAAGACGC CCATTTTCCT GAATGAAGCC
    TTGGTAAAAC TGCCCACAGA CCCTTCCAGC GACGAGCCTG TCTTCCACAT CTCTCACATT
    GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACGGCCTG GGTCCAGAAG
    ATCAAGGCCG CGTCTGAGCA GTACATCGAC ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT
    CAAGCTCGCT CTCAGAAGTC TTCAGGCATC GGGCGTCTGA TGGTGCATGT CATTGAAGCT
    ACAGAACTGA AAGCCTGTAA ACCAAATGGA AAGAGCAATC CGTACTGTGA AATCAGCATG
    GGCTCCCAGA GCTACACCAC CAGAACCCTC CAGGACACAC TAAATCCCAA ATGGAATTTT
    AACTGCCAGT TCTTCATTAA GGACCTCTAT CAGGACGTGC TGTGTCTTAC CATGTTCGAC
    AGAGATCAGT TTTCACCAGA CGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT
    CGAACAGAAC AAGAAAGCAA AGGCCCTACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC
    ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220885
    Clone ID Related Accession (Same CDS sequence) XM_024999509.1 , XM_024999510.1
    Accession Version XM_024999510.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA
    ACACTGGACA CCATGCAGCC GGTGGAAAGG AAGAGGCAGG GCTACATCCA CGAGCTGATT
    GAGACGGAGG AGCGCTACGT GGACGACCTG CAGCTCGTCG TGGAGGTTTT TCAGAAACGA
    ATAACAGAGC CAGGTTTTCT CGCTGAAGGA GAGATGGCCC TGATCTTTGT TAACTGGAAG
    GAGCTCATCA TGGCTAACAC GAAGCTGCTG AAGGCGCTGC GGGTGCGGAA GAAGACCGGG
    GGTGAGAAGA TGCCCGTGCA CATGATCGGG GACATCCTGG CCGCTGAGCT GTCCCACATG
    CAGGCCTACA TCCGCTTCTG CAGCTGTCAG CTTAATGGAG CAGCCCTGTT ACAGCAGAAG
    ACAGACGAAC ACGCAGACTT CAAAGAATTT TTAAAGAAGT TGGCATCTGA CCCTCGGTGT
    AAAGGGATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGGATCAC CCGCTACCCA
    CTCCTCATCA GAAGCATTCT GGAGAACACC CCAGAGAATC ATGTAGACCA CTCCTCTCTG
    AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC TGCTCTCAAG TGAATGAAGG AGTTCGGGAG
    AAGGAAAATT CAGACCGACT GGAGTGGATC CAGACGCACG TGCAGTGCGA TGGGCTTGCG
    GAGCAACTTG TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT CCTGCACAGT
    GGAAAGCTGT ACAAGACCAA GAGCAACAAG GAGCTACACG GGTTCCTCTT CAACGACTTC
    CTGCTTCTCA CCTACATGGT CAAGCAGTTC GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC
    AGCTCAAAGT CCAGTGCTCA GTTCAAGATG TATAAGACGC CCATTTTCCT GAATGAAGCC
    TTGGTAAAAC TGCCCACAGA CCCTTCCAGC GACGAGCCTG TCTTCCACAT CTCTCACATT
    GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACGGCCTG GGTCCAGAAG
    ATCAAGGCCG CGTCTGAGCA GTACATCGAC ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT
    CAAGCTCGCT CTCAGAAGTC TTCAGGCATC GGGCGTCTGA TGGTGCATGT CATTGAAGCT
    ACAGAACTGA AAGCCTGTAA ACCAAATGGA AAGAGCAATC CGTACTGTGA AATCAGCATG
    GGCTCCCAGA GCTACACCAC CAGAACCCTC CAGGACACAC TAAATCCCAA ATGGAATTTT
    AACTGCCAGT TCTTCATTAA GGACCTCTAT CAGGACGTGC TGTGTCTTAC CATGTTCGAC
    AGAGATCAGT TTTCACCAGA CGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT
    CGAACAGAAC AAGAAAGCAA AGGCCCTACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC
    ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855278.1
    CDS31..4950
    Translation

    Target ORF information:

    RefSeq Version XM_024999510.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999510.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGCTA TTACCTCCGA AGAACGGACT AAACATGACA AGCAGTTTGA TAACCTCAAA 
    CCTTCGGGAG GTTACATAAC AGGTGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCCG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAACAAG AGTTCTCCAT AGCGATGAAA CTCATCAAAC TAAAGCTTCA AGGCCAACAG
    TTGCCAGTGG TTCTTCCTCC TATTATGAAA CAGCCTCCTA TGTTCTCTCC ATTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGTCATT GCCTCCAGTT
    GCACCCATAG CACCACCCTT GTCCTCGGCG ACTTCAGGGA CCACCCTCCC TTCCCTATTG
    ATGGCTGCTC CCCTAGTGCC TTCCATTAGC ACTTCATCAT TACCAAATGG AACCGTCAGT
    CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC
    AGTCTGATGA TTGGAGGATT TGGTGGTGCT AATATACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAATTC ACCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATATGGA CTCTGGCTGA CATCGATGGT
    GATGGACAGC TGAAAGCTGA GGAGTTCATC CTTGCGATGC ACCTCACTGA TATGGCCAAA
    GCTGGACAGC CGTTGCCGCT GGCTTTACCT CCTGAGCTTG TCCCTCCATC TTTTAGAGGA
    GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACATTC GAGGACAAGC GGAAAGCCAA CTATGAGCGG
    GGCAACATGG AACTGGAGAA GCGACGCCAG GCCATGATGG AGCAGCAGCA GAGGGAGGCA
    GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA TGGGAACGAA AACAGAGAGA GCTACAAGAG
    CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCCTGG AGAAGCAGAG GGAATTGGAG
    CGACAACGGG AAGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCCGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGAATTCGTA GGCAGGAGCT TCTCAGTCGA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG CCTCCATCTC
    GAGTTGGAAG CACTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCAGAAAGC AAACTCAGAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATCATGGAAA TCAAGCAGCT TCAACAGGAG CTTCAGGAGT ATCAAAATAA ACTTATCTAT
    TTGGTGCCAG AGAAGCAGTT ATTAAATGAA AGAATTAAAA ACATGCAGCT CGGTAACACA
    CCTGATTTAG GGATCAGTTT ACTTCAAAAA AAATCATTAG AAAAAGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAG AAAGAAACTG CATCCAAGCT TTCAGAAATG
    GATTCATTTA ACAATGAACT GAAGGAACTG AGAGAAAGTT ACAATACACA GCAGTTAGCC
    ATTGAACAGC TTTATAAGAT CAAACGTGAC AAACTGAGGG AAATTGAAAG AAAAAGATCA
    GAGTTAATAC AGAAAAAGAA ACTAGAAGAT GAGGCTACAA GGAAAGCAAA ACAAGGAAAA
    GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA ACGACTCCAA
    GAAGAAAAAG CACAGGAAAA AGTTCAAGAA GTGGAGCGGA AAGCTGAGGA GAAACAAAGT
    GAGCCAGCTG GTACTTTGGT GAATTACAGA GCGTTATATC GCTTTGAAGC AAGGAACCAT
    GATGAAATGA GTTTTAATTC TGGGGATATA ATACAGGTTG ATGAAAAAAC TGTAGGAGAA
    CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCAGG CAATTATGTA
    GAAAAAGTGA CATCAAGTGA AAAATCTGTG TCTCCTAAGA AGGCCTTACT TCCTCCTACA
    GTATCTGCTA CCTCAACTTC TGAATCACTT TCTTCAAACC AACCAGCATC TGTGATCGAT
    TATCGAAATG TATCTTTTTC AAACCTAAGT GTTAACACAT CATGGCAGAA AAAATCAGCT
    TTTACTCGCA CTGTGTCCCC TGTGTCTGTA TCACCGATTC ATGGACAGGG ACAGGTTGTA
    GAAAACCTGA AAGCACAGGC CCTTTGTTCT TGGACTGCAA AGAAAGAAAA CCACTTGAAC
    TTCTCAAAAC ATGATATTAT TACTGTCTTA GAGCAGCAAG AAAATTGGTG GTTTGGGGAG
    GTTCACGGAG GAAGAGGATG GTTTCCGAAA TCTTACGTCA AAATCATCCC TGGGAGTGAA
    GTTAAGCGGG AAGAACCAGA AGCTTTGTAT GCATCTGTAA ACAAGAAACC TGCCTCTGCA
    GCCTGTACAG TGGGAGAAGA GTATATTGCA CTTTATTCAT ATTCAAGTGT AGAACCTGGA
    GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG
    ACAGGAAGTA TTGGAGACAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT
    CAGGAGGGTT TTGGGAGTTC TAGCAAATCT GGAACATCAA ATAAGAAACC TGAGATTGCT
    CAAGTAACAT CAGCATATGC GGCTTCTGGT TCTGAACAGC TTAGCCTCGC ACCAGGACAG
    TTAATACTAA TTCTAAAGAA AAACACAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA
    GGGAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT
    AGTGAAAGAA CTGCGCCTGC CTTTCATCCT GTGTGTCAGG TGATCGCCAT GTATGACTAC
    ACGGCAAACA ACGAGGACGA GCTCAGTTTC TCCAAGGGAC AGCTTATTAA TGTTCTAAAC
    AAGGATGATC CTGACTGGTG GCAAGGAGAA ACCAGTGGGG TGACTGGTCT CTTCCCTTCA
    AACTACGTTA AGATGACGAC AGATGCAGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA
    ACACTGGACA CCATGCAGCC GGTGGAAAGG AAGAGGCAGG GCTACATCCA CGAGCTGATT
    GAGACGGAGG AGCGCTACGT GGACGACCTG CAGCTCGTCG TGGAGGTTTT TCAGAAACGA
    ATAACAGAGC CAGGTTTTCT CGCTGAAGGA GAGATGGCCC TGATCTTTGT TAACTGGAAG
    GAGCTCATCA TGGCTAACAC GAAGCTGCTG AAGGCGCTGC GGGTGCGGAA GAAGACCGGG
    GGTGAGAAGA TGCCCGTGCA CATGATCGGG GACATCCTGG CCGCTGAGCT GTCCCACATG
    CAGGCCTACA TCCGCTTCTG CAGCTGTCAG CTTAATGGAG CAGCCCTGTT ACAGCAGAAG
    ACAGACGAAC ACGCAGACTT CAAAGAATTT TTAAAGAAGT TGGCATCTGA CCCTCGGTGT
    AAAGGGATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGGATCAC CCGCTACCCA
    CTCCTCATCA GAAGCATTCT GGAGAACACC CCAGAGAATC ATGTAGACCA CTCCTCTCTG
    AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC TGCTCTCAAG TGAATGAAGG AGTTCGGGAG
    AAGGAAAATT CAGACCGACT GGAGTGGATC CAGACGCACG TGCAGTGCGA TGGGCTTGCG
    GAGCAACTTG TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT CCTGCACAGT
    GGAAAGCTGT ACAAGACCAA GAGCAACAAG GAGCTACACG GGTTCCTCTT CAACGACTTC
    CTGCTTCTCA CCTACATGGT CAAGCAGTTC GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC
    AGCTCAAAGT CCAGTGCTCA GTTCAAGATG TATAAGACGC CCATTTTCCT GAATGAAGCC
    TTGGTAAAAC TGCCCACAGA CCCTTCCAGC GACGAGCCTG TCTTCCACAT CTCTCACATT
    GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACGGCCTG GGTCCAGAAG
    ATCAAGGCCG CGTCTGAGCA GTACATCGAC ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT
    CAAGCTCGCT CTCAGAAGTC TTCAGGCATC GGGCGTCTGA TGGTGCATGT CATTGAAGCT
    ACAGAACTGA AAGCCTGTAA ACCAAATGGA AAGAGCAATC CGTACTGTGA AATCAGCATG
    GGCTCCCAGA GCTACACCAC CAGAACCCTC CAGGACACAC TAAATCCCAA ATGGAATTTT
    AACTGCCAGT TCTTCATTAA GGACCTCTAT CAGGACGTGC TGTGTCTTAC CATGTTCGAC
    AGAGATCAGT TTTCACCAGA CGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT
    CGAACAGAAC AAGAAAGCAA AGGCCCTACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC
    ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91949
    Clone ID Related Accession (Same CDS sequence) XM_015465428.1 , XM_015473567.1 , XM_024999511.1
    Accession Version XM_015465428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320914.1
    CDS236..4810
    Translation

    Target ORF information:

    RefSeq Version XM_015465428.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91949
    Clone ID Related Accession (Same CDS sequence) XM_015465428.1 , XM_015473567.1 , XM_024999511.1
    Accession Version XM_015473567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329053.1
    CDS236..4810
    Translation

    Target ORF information:

    RefSeq Version XM_015473567.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91949
    Clone ID Related Accession (Same CDS sequence) XM_015465428.1 , XM_015473567.1 , XM_024999511.1
    Accession Version XM_024999511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855279.1
    CDS169..4743
    Translation

    Target ORF information:

    RefSeq Version XM_024999511.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGGTTT CCCTTATTTT
    GTTACATACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810285.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808587.1
    CDS236..5197
    Translation

    Target ORF information:

    RefSeq Version XM_010810285.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810286.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808588.1
    CDS167..5128
    Translation

    Target ORF information:

    RefSeq Version XM_010810286.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_015465425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320911.1
    CDS167..5128
    Translation

    Target ORF information:

    RefSeq Version XM_015465425.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810288.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808590.1
    CDS136..5097
    Translation

    Target ORF information:

    RefSeq Version XM_010810288.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_015465423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320909.1
    CDS236..5197
    Translation

    Target ORF information:

    RefSeq Version XM_015465423.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_015465424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320910.1
    CDS130..5091
    Translation

    Target ORF information:

    RefSeq Version XM_015465424.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810286.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808588.1
    CDS219..5180
    Translation

    Target ORF information:

    RefSeq Version XM_010810286.3
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810286.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_015465426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320912.1
    CDS136..5097
    Translation

    Target ORF information:

    RefSeq Version XM_015465426.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810287.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808589.1
    CDS130..5091
    Translation

    Target ORF information:

    RefSeq Version XM_010810287.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810287.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808589.1
    CDS169..5130
    Translation

    Target ORF information:

    RefSeq Version XM_010810287.3
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810287.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50445
    Clone ID Related Accession (Same CDS sequence) XM_010810285.2 , XM_010810286.2 , XM_015465425.1 , XM_010810288.2 , XM_015465423.1 , XM_015465424.1 , XM_010810286.3 , XM_015465426.1 , XM_010810287.2 , XM_010810287.3 , XM_010810285.3
    Accession Version XM_010810285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808587.1
    CDS259..5220
    Translation

    Target ORF information:

    RefSeq Version XM_010810285.3
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGGAAC TGAGAGAAAG TTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG
    ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG
    AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA
    AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA
    AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG
    GTGAATTACA GAGCGTTATA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT
    TCTGGGGATA TAATACAGGT TGATGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCA GGCAATTATG TAGAAAAAGT GACATCAAGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT
    TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGATCG ATTATCGAAA TGTATCTTTT
    TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC
    CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TAGAAAACCT GAAAGCACAG
    GCCCTTTGTT CTTGGACTGC AAAGAAAGAA AACCACTTGA ACTTCTCAAA ACATGATATT
    ATTACTGTCT TAGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA
    TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA
    GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTGCCTCTG CAGCCTGTAC AGTGGGAGAA
    GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA
    GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC
    AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT
    TCTAGCAAAT CTGGAACATC AAATAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT
    GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG
    AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA
    GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTGCGCCT
    GCCTTTCATC CTGTGTGTCA GGTGATCGCC ATGTATGACT ACACGGCAAA CAACGAGGAC
    GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTCTAA ACAAGGATGA TCCTGACTGG
    TGGCAAGGAG AAACCAGTGG GGTGACTGGT CTCTTCCCTT CAAACTACGT TAAGATGACG
    ACAGATGCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG
    CCGGTGGAAA GGAAGAGGCA GGGCTACATC CACGAGCTGA TTGAGACGGA GGAGCGCTAC
    GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT
    CTCGCTGAAG GAGAGATGGC CCTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC
    ACGAAGCTGC TGAAGGCGCT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG
    CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC
    TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC
    TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC
    AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT
    CTGGAGAACA CCCCAGAGAA TCATGTAGAC CACTCCTCTC TGAAGCTGGC CCTTGAGCGG
    GCAGAGGAGC TCTGCTCTCA AGTGAATGAA GGAGTTCGGG AGAAGGAAAA TTCAGACCGA
    CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTTG CGGAGCAACT TGTTTTCAAC
    TCCCTCACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GTGGAAAGCT GTACAAGACC
    AAGAGCAACA AGGAGCTACA CGGGTTCCTC TTCAACGACT TCCTGCTTCT CACCTACATG
    GTCAAGCAGT TCGCAGTCTC CTCGGCCTCC GAGAAGCTTT TCAGCTCAAA GTCCAGTGCT
    CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAATGAAG CCTTGGTAAA ACTGCCCACA
    GACCCTTCCA GCGACGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC
    CGAACAGACA ACATTAACGA GAGGACGGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG
    CAGTACATCG ACACCGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCTCG CTCTCAGAAG
    TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT
    AAACCAAATG GAAAGAGCAA TCCGTACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC
    ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT
    AAGGACCTCT ATCAGGACGT GCTGTGTCTT ACCATGTTCG ACAGAGATCA GTTTTCACCA
    GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAAGAAAGC
    AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACCGGGGA GGTCTGGGTC
    CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50446
    Clone ID Related Accession (Same CDS sequence) XM_010810289.2 , XM_010810289.3 , XM_015465427.1
    Accession Version XM_010810289.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808591.1
    CDS229..5178
    Translation

    Target ORF information:

    RefSeq Version XM_010810289.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810289.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50446
    Clone ID Related Accession (Same CDS sequence) XM_010810289.2 , XM_010810289.3 , XM_015465427.1
    Accession Version XM_010810289.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808591.1
    CDS1..4950
    Translation

    Target ORF information:

    RefSeq Version XM_010810289.3
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810289.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50446
    Clone ID Related Accession (Same CDS sequence) XM_010810289.2 , XM_010810289.3 , XM_015465427.1
    Accession Version XM_015465427.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320913.1
    CDS229..5178
    Translation

    Target ORF information:

    RefSeq Version XM_015465427.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGA CCTTGAAAGG AGGGCCAAAC ATGTGGGCTA TTACCTCCGA AGAACGGACT 
    AAACATGACA AGCAGTTTGA TAACCTCAAA CCTTCGGGAG GTTACATAAC AGGTGATCAA
    GCCCGTACCT TTTTCTTACA GTCAGGCCTG CCGGCTCCCG TTTTAGCTGA AATATGGGCT
    TTATCAGACC TGAACAAGGA TGGGAAGATG GATCAACAAG AGTTCTCCAT AGCGATGAAA
    CTCATCAAAC TAAAGCTTCA AGGCCAACAG TTGCCAGTGG TTCTTCCTCC TATTATGAAA
    CAGCCTCCTA TGTTCTCTCC ATTAATTTCT GCTCGTTTTG GAATGGGAAG CATGCCCAAT
    CTGTCCATTC CTCAGTCATT GCCTCCAGTT GCACCCATAG CACCACCCTT GTCCTCGGCG
    ACTTCAGGGA CCACCCTCCC TTCCCTATTG ATGGCTGCTC CCCTAGTGCC TTCCATTAGC
    ACTTCATCAT TACCAAATGG AACCGTCAGT CTCATTCAGC CTTTATCCAT TCCTTATTCT
    TCTTCAACAT TGCCTCATAC GTCATCTTAC AGTCTGATGA TTGGAGGATT TGGTGGTGCT
    AATATACAGA AAGCCCAGTC TCTGATTGAT TTAGGATCTA GTAGCTCAAC TTCCTCAACT
    GCTTCCCTCT CAGGGAATTC ACCCAAGACT GGGACCTCAG AGTGGGCAGT TCCTCAGCCT
    TCAAGATTAA AATATCGGCA AAAATTTAAT AGTCTTGACA AAAGCATGAG TGGATACCTC
    TCAGGTTTTC AAGCTAGAAA TGCTCTTCTT CAGTCAAATC TTTCTCAAAC TCAGCTAGCT
    ACTATATGGA CTCTGGCTGA CATCGATGGT GATGGACAGC TGAAAGCTGA GGAGTTCATC
    CTTGCGATGC ACCTCACTGA TATGGCCAAA GCTGGACAGC CGTTGCCGCT GGCTTTACCT
    CCTGAGCTTG TCCCTCCATC TTTTAGAGGA GGAAAGCAAA TTGATTCCAT TAATGGAACT
    CTGCCTTCAT ATCAGAAAGC ACAAGAAGAG GAACCTCAGA AAAAATTACC AGTTACATTC
    GAGGACAAGC GGAAAGCCAA CTATGAGCGG GGCAACATGG AACTGGAGAA GCGACGCCAG
    GCCATGATGG AGCAGCAGCA GAGGGAGGCA GAGCGCAAAG CCCAGAAGGA AAAGGAAGAA
    TGGGAACGAA AACAGAGAGA GCTACAAGAG CAAGAATGGA AGAAACAACT TGAATTAGAA
    AAACGCCTGG AGAAGCAGAG GGAATTGGAG CGACAACGGG AAGAAGAAAG GAGAAAAGAG
    ATAGAAAGAC GAGAGGCCGC AAAACAGGAA CTTGAAAGAC AACGTCGTTT AGAATGGGAG
    AGAATTCGTA GGCAGGAGCT TCTCAGTCGA AGGAATAGAG AACAGGAAGA AATTGTCAGG
    TTAAACTCTA AAAAGAAGAG CCTCCATCTC GAGTTGGAAG CACTGAATGG CAAACATCAG
    CAGATCTCAG GCAGACTTCA AGATGTCCGA CTCAGAAAGC AAACTCAGAA GACTGAGCTG
    GAAGTTCTGG ATAAGCAGTG TGACCTGGAA ATCATGGAAA TCAAGCAGCT TCAACAGGAG
    CTTCAGGAGT ATCAAAATAA ACTTATCTAT TTGGTGCCAG AGAAGCAGTT ATTAAATGAA
    AGAATTAAAA ACATGCAGCT CGGTAACACA CCTGATTTAG GGATCAGTTT ACTTCAAAAA
    AAATCATTAG AAAAAGAAGA ATTATGCCAA AGACTTAAAG AACAATTAGA TGCTCTTGAG
    AAAGAAACTG CATCCAAGCT TTCAGAAATG GATTCATTTA ACAATGAACT GAAGGAACTG
    AGAGAAAGTT ACAATACACA GCAGTTAGCC ATTGAACAGC TTTATAAGAT CAAACGTGAC
    AAACTGAGGG AAATTGAAAG AAAAAGATCA GAGTTAATAC AGAAAAAGAA ACTAGAAGAT
    GAGGCTACAA GGAAAGCAAA ACAAGGAAAA GAAAACTTAT GGAAAGAAAA TCTTAGAAAG
    GAGGAAGAAG AAAAACAAAA ACGACTCCAA GAAGAAAAAG CACAGGAAAA AGTTCAAGAA
    GTGGAGCGGA AAGCTGAGGA GAAACAAAGT GAGCCAGCTG GTACTTTGGT GAATTACAGA
    GCGTTATATC GCTTTGAAGC AAGGAACCAT GATGAAATGA GTTTTAATTC TGGGGATATA
    ATACAGGTTG ATGAAAAAAC TGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA
    AATTTTGGCT GGTTTCCAGG CAATTATGTA GAAAAAGTGA CATCAAGTGA AAAATCTGTG
    TCTCCTAAGA AGGCCTTACT TCCTCCTACA GTATCTGCTA CCTCAACTTC TGAATCACTT
    TCTTCAAACC AACCAGCATC TGTGATCGAT TATCGAAATG TATCTTTTTC AAACCTAAGT
    GTTAACACAT CATGGCAGAA AAAATCAGCT TTTACTCGCA CTGTGTCCCC TGTGTCTGTA
    TCACCGATTC ATGGACAGGG ACAGGTTGTA GAAAACCTGA AAGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGAAAA CCACTTGAAC TTCTCAAAAC ATGATATTAT TACTGTCTTA
    GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTTCACGGAG GAAGAGGATG GTTTCCGAAA
    TCTTACGTCA AAATCATCCC TGGGAGTGAA GTTAAGCGGG AAGAACCAGA AGCTTTGTAT
    GCATCTGTAA ACAAGAAACC TGCCTCTGCA GCCTGTACAG TGGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    TTGGTGACCC AGAAAGATGG AGAATGGTGG ACAGGAAGTA TTGGAGACAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAGGAGGGTT TTGGGAGTTC TAGCAAATCT
    GGAACATCAA ATAAGAAACC TGAGATTGCT CAAGTAACAT CAGCATATGC GGCTTCTGGT
    TCTGAACAGC TTAGCCTCGC ACCAGGACAG TTAATACTAA TTCTAAAGAA AAACACAAGT
    GGGTGGTGGC AAGGGGAATT ACAGGCCAGA GGGAAAAAGC GACAGAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAA CTGCGCCTGC CTTTCATCCT
    GTGTGTCAGG TGATCGCCAT GTATGACTAC ACGGCAAACA ACGAGGACGA GCTCAGTTTC
    TCCAAGGGAC AGCTTATTAA TGTTCTAAAC AAGGATGATC CTGACTGGTG GCAAGGAGAA
    ACCAGTGGGG TGACTGGTCT CTTCCCTTCA AACTACGTTA AGATGACGAC AGATGCAGAT
    CCAAGTCAAC AGTGGTGTGC TGATCTCCAA ACACTGGACA CCATGCAGCC GGTGGAAAGG
    AAGAGGCAGG GCTACATCCA CGAGCTGATT GAGACGGAGG AGCGCTACGT GGACGACCTG
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGA ATAACAGAGC CAGGTTTTCT CGCTGAAGGA
    GAGATGGCCC TGATCTTTGT TAACTGGAAG GAGCTCATCA TGGCTAACAC GAAGCTGCTG
    AAGGCGCTGC GGGTGCGGAA GAAGACCGGG GGTGAGAAGA TGCCCGTGCA CATGATCGGG
    GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCCTACA TCCGCTTCTG CAGCTGTCAG
    CTTAATGGAG CAGCCCTGTT ACAGCAGAAG ACAGACGAAC ACGCAGACTT CAAAGAATTT
    TTAAAGAAGT TGGCATCTGA CCCTCGGTGT AAAGGGATGC CCCTTTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCA CTCCTCATCA GAAGCATTCT GGAGAACACC
    CCAGAGAATC ATGTAGACCA CTCCTCTCTG AAGCTGGCCC TTGAGCGGGC AGAGGAGCTC
    TGCTCTCAAG TGAATGAAGG AGTTCGGGAG AAGGAAAATT CAGACCGACT GGAGTGGATC
    CAGACGCACG TGCAGTGCGA TGGGCTTGCG GAGCAACTTG TTTTCAACTC CCTCACCAAC
    TGCCTGGGGC CCCGGAAGCT CCTGCACAGT GGAAAGCTGT ACAAGACCAA GAGCAACAAG
    GAGCTACACG GGTTCCTCTT CAACGACTTC CTGCTTCTCA CCTACATGGT CAAGCAGTTC
    GCAGTCTCCT CGGCCTCCGA GAAGCTTTTC AGCTCAAAGT CCAGTGCTCA GTTCAAGATG
    TATAAGACGC CCATTTTCCT GAATGAAGCC TTGGTAAAAC TGCCCACAGA CCCTTCCAGC
    GACGAGCCTG TCTTCCACAT CTCTCACATT GACCGGGTCT ACACCCTCCG AACAGACAAC
    ATTAACGAGA GGACGGCCTG GGTCCAGAAG ATCAAGGCCG CGTCTGAGCA GTACATCGAC
    ACCGAAAAGA AGAAGCGGGA GAAGGCCTAT CAAGCTCGCT CTCAGAAGTC TTCAGGCATC
    GGGCGTCTGA TGGTGCATGT CATTGAAGCT ACAGAACTGA AAGCCTGTAA ACCAAATGGA
    AAGAGCAATC CGTACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGAACCCTC
    CAGGACACAC TAAATCCCAA ATGGAATTTT AACTGCCAGT TCTTCATTAA GGACCTCTAT
    CAGGACGTGC TGTGTCTTAC CATGTTCGAC AGAGATCAGT TTTCACCAGA CGATTTCCTG
    GGTCGCACTG AAGTTCCAGT GGCAAAAATT CGAACAGAAC AAGAAAGCAA AGGCCCTACA
    ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC ACCGGGGAGG TCTGGGTCCG CTTTGACCTG
    CAGCTTTTTG AGCAGAAAAC TCTCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50447
    Clone ID Related Accession (Same CDS sequence) XM_015465429.1 , XM_010810290.2
    Accession Version XM_015465429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGTGTG GGAATATGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GTTACAATAC ACAGCAGTTA
    GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA
    TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA
    AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC
    CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA
    AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCGTTAT ATCGCTTTGA AGCAAGGAAC
    CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGATGAAAA AACTGTAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC AGGCAATTAT
    GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT
    ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGATC
    GATTATCGAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA
    GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT
    GTAGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACTG CAAAGAAAGA AAACCACTTG
    AACTTCTCAA AACATGATAT TATTACTGTC TTAGAGCAGC AAGAAAATTG GTGGTTTGGG
    GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT
    GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTGCCTCT
    GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT
    GGAGATTTGA CTTTCACTGA AGGTGAAGAA ATATTGGTGA CCCAGAAAGA TGGAGAATGG
    TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA
    GATCAGGAGG GTTTTGGGAG TTCTAGCAAA TCTGGAACAT CAAATAAGAA ACCTGAGATT
    GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA
    CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC
    AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA
    AGTAGTGAAA GAACTGCGCC TGCCTTTCAT CCTGTGTGTC AGGTGATCGC CATGTATGAC
    TACACGGCAA ACAACGAGGA CGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTCTA
    AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAACCAGTG GGGTGACTGG TCTCTTCCCT
    TCAAACTACG TTAAGATGAC GACAGATGCA GATCCAAGTC AACAGTGGTG TGCTGATCTC
    CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCACGAGCTG
    ATTGAGACGG AGGAGCGCTA CGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA
    CGAATAACAG AGCCAGGTTT TCTCGCTGAA GGAGAGATGG CCCTGATCTT TGTTAACTGG
    AAGGAGCTCA TCATGGCTAA CACGAAGCTG CTGAAGGCGC TGCGGGTGCG GAAGAAGACC
    GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC
    ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG
    AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG
    TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC
    CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCATGTAGA CCACTCCTCT
    CTGAAGCTGG CCCTTGAGCG GGCAGAGGAG CTCTGCTCTC AAGTGAATGA AGGAGTTCGG
    GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTT
    GCGGAGCAAC TTGTTTTCAA CTCCCTCACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC
    AGTGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTAC ACGGGTTCCT CTTCAACGAC
    TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTCGCAGTCT CCTCGGCCTC CGAGAAGCTT
    TTCAGCTCAA AGTCCAGTGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAATGAA
    GCCTTGGTAA AACTGCCCAC AGACCCTTCC AGCGACGAGC CTGTCTTCCA CATCTCTCAC
    ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACGGC CTGGGTCCAG
    AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACCGAAA AGAAGAAGCG GGAGAAGGCC
    TATCAAGCTC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA
    GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCGTACTG TGAAATCAGC
    ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT
    TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGACG TGCTGTGTCT TACCATGTTC
    GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA
    ATTCGAACAG AACAAGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC
    CCCACCGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320915.1
    CDS237..5279
    Translation

    Target ORF information:

    RefSeq Version XM_015465429.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGTGTG GGAATATGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GTTACAATAC ACAGCAGTTA
    GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA
    TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA
    AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC
    CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA
    AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCGTTAT ATCGCTTTGA AGCAAGGAAC
    CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGATGAAAA AACTGTAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC AGGCAATTAT
    GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT
    ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGATC
    GATTATCGAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA
    GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT
    GTAGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACTG CAAAGAAAGA AAACCACTTG
    AACTTCTCAA AACATGATAT TATTACTGTC TTAGAGCAGC AAGAAAATTG GTGGTTTGGG
    GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT
    GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTGCCTCT
    GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT
    GGAGATTTGA CTTTCACTGA AGGTGAAGAA ATATTGGTGA CCCAGAAAGA TGGAGAATGG
    TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA
    GATCAGGAGG GTTTTGGGAG TTCTAGCAAA TCTGGAACAT CAAATAAGAA ACCTGAGATT
    GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA
    CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC
    AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA
    AGTAGTGAAA GAACTGCGCC TGCCTTTCAT CCTGTGTGTC AGGTGATCGC CATGTATGAC
    TACACGGCAA ACAACGAGGA CGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTCTA
    AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAACCAGTG GGGTGACTGG TCTCTTCCCT
    TCAAACTACG TTAAGATGAC GACAGATGCA GATCCAAGTC AACAGTGGTG TGCTGATCTC
    CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCACGAGCTG
    ATTGAGACGG AGGAGCGCTA CGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA
    CGAATAACAG AGCCAGGTTT TCTCGCTGAA GGAGAGATGG CCCTGATCTT TGTTAACTGG
    AAGGAGCTCA TCATGGCTAA CACGAAGCTG CTGAAGGCGC TGCGGGTGCG GAAGAAGACC
    GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC
    ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG
    AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG
    TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC
    CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCATGTAGA CCACTCCTCT
    CTGAAGCTGG CCCTTGAGCG GGCAGAGGAG CTCTGCTCTC AAGTGAATGA AGGAGTTCGG
    GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTT
    GCGGAGCAAC TTGTTTTCAA CTCCCTCACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC
    AGTGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTAC ACGGGTTCCT CTTCAACGAC
    TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTCGCAGTCT CCTCGGCCTC CGAGAAGCTT
    TTCAGCTCAA AGTCCAGTGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAATGAA
    GCCTTGGTAA AACTGCCCAC AGACCCTTCC AGCGACGAGC CTGTCTTCCA CATCTCTCAC
    ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACGGC CTGGGTCCAG
    AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACCGAAA AGAAGAAGCG GGAGAAGGCC
    TATCAAGCTC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA
    GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCGTACTG TGAAATCAGC
    ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT
    TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGACG TGCTGTGTCT TACCATGTTC
    GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA
    ATTCGAACAG AACAAGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC
    CCCACCGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50447
    Clone ID Related Accession (Same CDS sequence) XM_015465429.1 , XM_010810290.2
    Accession Version XM_010810290.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product intersectin-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGTGTG GGAATATGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GTTACAATAC ACAGCAGTTA
    GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA
    TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA
    AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC
    CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA
    AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCGTTAT ATCGCTTTGA AGCAAGGAAC
    CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGATGAAAA AACTGTAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC AGGCAATTAT
    GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT
    ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGATC
    GATTATCGAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA
    GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT
    GTAGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACTG CAAAGAAAGA AAACCACTTG
    AACTTCTCAA AACATGATAT TATTACTGTC TTAGAGCAGC AAGAAAATTG GTGGTTTGGG
    GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT
    GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTGCCTCT
    GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT
    GGAGATTTGA CTTTCACTGA AGGTGAAGAA ATATTGGTGA CCCAGAAAGA TGGAGAATGG
    TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA
    GATCAGGAGG GTTTTGGGAG TTCTAGCAAA TCTGGAACAT CAAATAAGAA ACCTGAGATT
    GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA
    CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC
    AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA
    AGTAGTGAAA GAACTGCGCC TGCCTTTCAT CCTGTGTGTC AGGTGATCGC CATGTATGAC
    TACACGGCAA ACAACGAGGA CGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTCTA
    AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAACCAGTG GGGTGACTGG TCTCTTCCCT
    TCAAACTACG TTAAGATGAC GACAGATGCA GATCCAAGTC AACAGTGGTG TGCTGATCTC
    CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCACGAGCTG
    ATTGAGACGG AGGAGCGCTA CGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA
    CGAATAACAG AGCCAGGTTT TCTCGCTGAA GGAGAGATGG CCCTGATCTT TGTTAACTGG
    AAGGAGCTCA TCATGGCTAA CACGAAGCTG CTGAAGGCGC TGCGGGTGCG GAAGAAGACC
    GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC
    ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG
    AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG
    TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC
    CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCATGTAGA CCACTCCTCT
    CTGAAGCTGG CCCTTGAGCG GGCAGAGGAG CTCTGCTCTC AAGTGAATGA AGGAGTTCGG
    GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTT
    GCGGAGCAAC TTGTTTTCAA CTCCCTCACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC
    AGTGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTAC ACGGGTTCCT CTTCAACGAC
    TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTCGCAGTCT CCTCGGCCTC CGAGAAGCTT
    TTCAGCTCAA AGTCCAGTGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAATGAA
    GCCTTGGTAA AACTGCCCAC AGACCCTTCC AGCGACGAGC CTGTCTTCCA CATCTCTCAC
    ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACGGC CTGGGTCCAG
    AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACCGAAA AGAAGAAGCG GGAGAAGGCC
    TATCAAGCTC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA
    GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCGTACTG TGAAATCAGC
    ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT
    TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGACG TGCTGTGTCT TACCATGTTC
    GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA
    ATTCGAACAG AACAAGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC
    CCCACCGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808592.1
    CDS237..5279
    Translation

    Target ORF information:

    RefSeq Version XM_010810290.2
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 2 (ITSN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810290.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCC 
    GAAGAACGGA CTAAACATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTACATA
    ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA AGAGTTCTCC
    ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCAGT GGTTCTTCCT
    CCTATTATGA AACAGCCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCACCACCC
    TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTTCCCTAT TGATGGCTGC TCCCCTAGTG
    CCTTCCATTA GCACTTCATC ATTACCAAAT GGAACCGTCA GTCTCATTCA GCCTTTATCC
    ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATTGGAGGA
    TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATATG GACTCTGGCT GACATCGATG GTGATGGACA GCTGAAAGCT
    GAGGAGTTCA TCCTTGCGAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCGTTGCCG
    CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA AATTGATTCC
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA GCACAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACAT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG
    AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAG
    GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA
    CTTGAATTAG AAAAACGCCT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGTTGGA AGCACTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCAGAAA GCAAACTCAG
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG
    CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG
    TTATTAAATG AAAGAATTAA AAACATGCAG CTCGGTAACA CACCTGATTT AGGGATCAGT
    TTACTTCAAA AAAAATCATT AGAAAAAGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AGAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA
    CTGAAGTGTG GGAATATGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GTTACAATAC ACAGCAGTTA
    GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA
    TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA
    AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC
    CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA
    AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCGTTAT ATCGCTTTGA AGCAAGGAAC
    CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGATGAAAA AACTGTAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC AGGCAATTAT
    GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT
    ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGATC
    GATTATCGAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA
    GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT
    GTAGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACTG CAAAGAAAGA AAACCACTTG
    AACTTCTCAA AACATGATAT TATTACTGTC TTAGAGCAGC AAGAAAATTG GTGGTTTGGG
    GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT
    GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTGCCTCT
    GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT
    GGAGATTTGA CTTTCACTGA AGGTGAAGAA ATATTGGTGA CCCAGAAAGA TGGAGAATGG
    TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA
    GATCAGGAGG GTTTTGGGAG TTCTAGCAAA TCTGGAACAT CAAATAAGAA ACCTGAGATT
    GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA
    CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC
    AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA
    AGTAGTGAAA GAACTGCGCC TGCCTTTCAT CCTGTGTGTC AGGTGATCGC CATGTATGAC
    TACACGGCAA ACAACGAGGA CGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTCTA
    AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAACCAGTG GGGTGACTGG TCTCTTCCCT
    TCAAACTACG TTAAGATGAC GACAGATGCA GATCCAAGTC AACAGTGGTG TGCTGATCTC
    CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCACGAGCTG
    ATTGAGACGG AGGAGCGCTA CGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA
    CGAATAACAG AGCCAGGTTT TCTCGCTGAA GGAGAGATGG CCCTGATCTT TGTTAACTGG
    AAGGAGCTCA TCATGGCTAA CACGAAGCTG CTGAAGGCGC TGCGGGTGCG GAAGAAGACC
    GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC
    ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG
    AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG
    TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC
    CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCATGTAGA CCACTCCTCT
    CTGAAGCTGG CCCTTGAGCG GGCAGAGGAG CTCTGCTCTC AAGTGAATGA AGGAGTTCGG
    GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTT
    GCGGAGCAAC TTGTTTTCAA CTCCCTCACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC
    AGTGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTAC ACGGGTTCCT CTTCAACGAC
    TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTCGCAGTCT CCTCGGCCTC CGAGAAGCTT
    TTCAGCTCAA AGTCCAGTGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAATGAA
    GCCTTGGTAA AACTGCCCAC AGACCCTTCC AGCGACGAGC CTGTCTTCCA CATCTCTCAC
    ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACGGC CTGGGTCCAG
    AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACCGAAA AGAAGAAGCG GGAGAAGGCC
    TATCAAGCTC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA
    GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCGTACTG TGAAATCAGC
    ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT
    TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGACG TGCTGTGTCT TACCATGTTC
    GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA
    ATTCGAACAG AACAAGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC
    CCCACCGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.