SPAST cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAST
Entrez Gene ID	521442
Full Name	spastin
Synonyms	SPG4
General protein information	Preferred Names spastin Names spastin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005212529.3	XP_005212586.1	spastin isoform X1
XM_005212530.3	XP_005212587.1	spastin isoform X2
XM_005212530.2	XP_005212587.1	spastin isoform X2
XM_005212529.2	XP_005212586.1	spastin isoform X1
XM_024998710.1	XP_024854478.1	spastin isoform X3
NM_001081591.1	NP_001075060.1	spastin

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Gene Ontology
Bibliography

Related articles in PubMed

Congenital bovine spinal dysmyelination is caused by a missense mutation in the SPAST gene.
Thomsen B, Nissen PH, Agerholm JS, Bendixen C
Neurogenetics11(2)175-83(2010 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate a causal relationship between the SPAST mutation and bovine spinal dysmyelination.
Title: Congenital bovine spinal dysmyelination is caused by a missense mutation in the SPAST gene.

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220368	XM_024998710.1 Latest version!	Bos taurus spastin (SPAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa43593	NM_001081591.1 Latest version!	Bos taurus spastin (SPAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa23843	XM_005212529.3 Latest version!	Bos taurus spastin (SPAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa23844	XM_005212530.3 Latest version!	Bos taurus spastin (SPAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OBa23844	XM_005212530.2	Bos taurus spastin (SPAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa23843	XM_005212529.2	Bos taurus spastin (SPAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAST ( XM_024998710.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854478.1 Bos taurus spastin (SPAST), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220368
Clone ID Related Accession (Same CDS sequence)	XM_024998710.1
Accession Version	XM_024998710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spastin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAAA GTGGAGCTGT TCCAAAAAGA 
AAAGATCCGT TAACACATGC TAGTAATTCA CTGCCTCGTT CAAAAACAGT TATGAAAACT 
GGACCCACAG GTCTTTCAGG CCACCATAGA GCACCTAGTT GCAGTGGTTT ATCCATGGTT 
TCTGGCGTGA GACAAGGACC TGGTTCTGCA GCTGCCACTC ATAAGAGTAC ACCAAAAACA 
AATAGAACAA ATAAGCCTTC TACCCCTACA ACTGCTGCTC GTAAAAAGAA AGACTTGAAG 
AATTTTAGGA ATGTGGACAG CAACCTTGCT AACCTTATAA TGAATGAAAT TGTGGACAAT 
GGAACAGCTG TTAAATTTGA TGATATAGCT GGTCAAGAGC TGGCAAAACA AGCATTGCAA 
GAAATTGTTA TTCTTCCTTC TCTGAGACCT GAGTTGTTCA CAGGGCTTAG GGCTCCTGCC 
AGAGGACTGT TACTCTTTGG CCCACCTGGA AATGGGAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAAT CTAATGCAAC CTTCTTTAAT ATAAGTGCTG CAAGTTTAAC TTCAAAATAT 
GTAGGAGAAG GAGAGAAATT GGTGAGAGCT CTATTTGCTG TGGCTCGAGA ACTTCAACCT 
TCCATAATTT TTATAGATGA AGTTGATAGT CTTTTGTGTG AAAGAAGAGA AGGAGAACAT 
GATGCTAGCA GACGTCTAAA AACTGAATTT CTAATAGAAT TTGATGGTGT ACAGTCTGCT 
GGAGATGACA GAGTGCTTGT AATGGGTGCA ACCAACAGGC CACAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTTACCAA ACGGGTTTAT GTGTCTTTGC CAAATGAGGA GACACGACTA 
CTTTTATTAA AAAATCTATT ATGTAAACAA GGAAGCCCAT TGACCCAAAA AGAACTAGCT 
CAACTTGCTA GAATGACCAA CGGATACTCA GGCAGTGATC TAACAGCTTT GGCAAAGGAT 
GCAGCACTGG GTCCTATCCG AGAACTGAAA CCAGAACAAG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACCGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGCCCTC AAACCTTAGA AGCATACATA CGTTGGAACA AGGACTTTGG AGATACCACT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854478.1
CDS	323..2068
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_024998710.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998710.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAAA GTGGAGCTGT TCCAAAAAGA 
AAAGATCCGT TAACACATGC TAGTAATTCA CTGCCTCGTT CAAAAACAGT TATGAAAACT 
GGACCCACAG GTCTTTCAGG CCACCATAGA GCACCTAGTT GCAGTGGTTT ATCCATGGTT 
TCTGGCGTGA GACAAGGACC TGGTTCTGCA GCTGCCACTC ATAAGAGTAC ACCAAAAACA 
AATAGAACAA ATAAGCCTTC TACCCCTACA ACTGCTGCTC GTAAAAAGAA AGACTTGAAG 
AATTTTAGGA ATGTGGACAG CAACCTTGCT AACCTTATAA TGAATGAAAT TGTGGACAAT 
GGAACAGCTG TTAAATTTGA TGATATAGCT GGTCAAGAGC TGGCAAAACA AGCATTGCAA 
GAAATTGTTA TTCTTCCTTC TCTGAGACCT GAGTTGTTCA CAGGGCTTAG GGCTCCTGCC 
AGAGGACTGT TACTCTTTGG CCCACCTGGA AATGGGAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAAT CTAATGCAAC CTTCTTTAAT ATAAGTGCTG CAAGTTTAAC TTCAAAATAT 
GTAGGAGAAG GAGAGAAATT GGTGAGAGCT CTATTTGCTG TGGCTCGAGA ACTTCAACCT 
TCCATAATTT TTATAGATGA AGTTGATAGT CTTTTGTGTG AAAGAAGAGA AGGAGAACAT 
GATGCTAGCA GACGTCTAAA AACTGAATTT CTAATAGAAT TTGATGGTGT ACAGTCTGCT 
GGAGATGACA GAGTGCTTGT AATGGGTGCA ACCAACAGGC CACAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTTACCAA ACGGGTTTAT GTGTCTTTGC CAAATGAGGA GACACGACTA 
CTTTTATTAA AAAATCTATT ATGTAAACAA GGAAGCCCAT TGACCCAAAA AGAACTAGCT 
CAACTTGCTA GAATGACCAA CGGATACTCA GGCAGTGATC TAACAGCTTT GGCAAAGGAT 
GCAGCACTGG GTCCTATCCG AGAACTGAAA CCAGAACAAG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACCGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGCCCTC AAACCTTAGA AGCATACATA CGTTGGAACA AGGACTTTGG AGATACCACT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( NM_001081591.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075060.1 Bos taurus spastin (SPAST), mRNA.

Price & Availability↑

CloneID	OBa43593
Clone ID Related Accession (Same CDS sequence)	NM_001081591.1
Accession Version	NM_001081591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	spastin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133327.1. On or before Feb 20, 2007 this sequence version replaced XM_001253711.1, XM_599706.3. ##Evidence-Data-START## Transcript exon combination :: BC133327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AGAAGCTGCA ACCAAGTTTG 
CAATTTTCCA AGTCACAAAC GGACGTCTAT AATGACAGTA CTAACTTGAC ATGCCGTAAT 
GGACATCTCC AGTCAGAAAG TGGAGCTGTT CCAAAAAGAA AAGATCCGTT AACACATGCT 
AGTAATTCAC TGCCTCGTTC AAAAACAGTT ATGAAAACTG GACCCACAGG TCTTTCAGGC 
CACCATAGAG CACCTAGTTG CAGTGGTTTA TCCATGGTTT CTGGCGTGAG ACAAGGACCT 
GGTTCTGCAG CTGCCACTCA TAAGAGTACA CCAAAAACAA ATAGAACAAA TAAGCCTTCT 
ACCCCTACAA CTGCTGCTCG TAAAAAGAAA GACTTGAAGA ATTTTAGGAA TGTGGACAGC 
AACCTTGCTA ACCTTATAAT GAATGAAATT GTGGACAATG GAACAGCTGT TAAATTTGAT 
GATATAGCTG GTCAAGAGCT GGCAAAACAA GCATTGCAAG AAATTGTTAT TCTTCCTTCT 
CTGAGACCTG AGTTGTTCAC AGGGCTTAGG GCTCCTGCCA GAGGACTGTT ACTCTTTGGC 
CCACCTGGAA ATGGGAAAAC AATGCTGGCT AAAGCAGTAG CTGCAGAATC TAATGCAACC 
TTCTTTAATA TAAGTGCTGC AAGTTTAACT TCAAAATATG TAGGAGAAGG AGAGAAATTG 
GTGAGAGCTC TATTTGCTGT GGCTCGAGAA CTTCAACCTT CCATAATTTT TATAGATGAA 
GTTGATAGTC TTTTGTGTGA AAGAAGAGAA GGAGAACATG ATGCTAGCAG ACGTCTAAAA 
ACTGAATTTC TAATAGAATT TGATGGTGTA CAGTCTGCTG GAGATGACAG AGTGCTTGTA 
ATGGGTGCAA CCAACAGGCC ACAAGAGCTT GATGAAGCTG TTCTCAGGCG TTTTACCAAA 
CGGGTTTATG TGTCTTTGCC AAATGAGGAG ACACGACTAC TTTTATTAAA AAATCTATTA 
TGTAAACAAG GAAGCCCATT GACCCAAAAA GAACTAGCTC AACTTGCTAG AATGACCAAC 
GGATACTCAG GCAGTGATCT AACAGCTTTG GCAAAGGATG CAGCACTGGG TCCTATCCGA 
GAACTGAAAC CAGAACAAGT GAAGAATATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CCGAATCCTT AAAAAAGATA AAACGCAGTG TGAGCCCTCA AACCTTAGAA 
GCATACATAC GTTGGAACAA GGACTTTGGA GATACCACTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075060.1
CDS	286..2130
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKVGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKLQPSLQFSKSQTDVYNDSTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001081591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081591.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AGAAGCTGCA ACCAAGTTTG 
CAATTTTCCA AGTCACAAAC GGACGTCTAT AATGACAGTA CTAACTTGAC ATGCCGTAAT 
GGACATCTCC AGTCAGAAAG TGGAGCTGTT CCAAAAAGAA AAGATCCGTT AACACATGCT 
AGTAATTCAC TGCCTCGTTC AAAAACAGTT ATGAAAACTG GACCCACAGG TCTTTCAGGC 
CACCATAGAG CACCTAGTTG CAGTGGTTTA TCCATGGTTT CTGGCGTGAG ACAAGGACCT 
GGTTCTGCAG CTGCCACTCA TAAGAGTACA CCAAAAACAA ATAGAACAAA TAAGCCTTCT 
ACCCCTACAA CTGCTGCTCG TAAAAAGAAA GACTTGAAGA ATTTTAGGAA TGTGGACAGC 
AACCTTGCTA ACCTTATAAT GAATGAAATT GTGGACAATG GAACAGCTGT TAAATTTGAT 
GATATAGCTG GTCAAGAGCT GGCAAAACAA GCATTGCAAG AAATTGTTAT TCTTCCTTCT 
CTGAGACCTG AGTTGTTCAC AGGGCTTAGG GCTCCTGCCA GAGGACTGTT ACTCTTTGGC 
CCACCTGGAA ATGGGAAAAC AATGCTGGCT AAAGCAGTAG CTGCAGAATC TAATGCAACC 
TTCTTTAATA TAAGTGCTGC AAGTTTAACT TCAAAATATG TAGGAGAAGG AGAGAAATTG 
GTGAGAGCTC TATTTGCTGT GGCTCGAGAA CTTCAACCTT CCATAATTTT TATAGATGAA 
GTTGATAGTC TTTTGTGTGA AAGAAGAGAA GGAGAACATG ATGCTAGCAG ACGTCTAAAA 
ACTGAATTTC TAATAGAATT TGATGGTGTA CAGTCTGCTG GAGATGACAG AGTGCTTGTA 
ATGGGTGCAA CCAACAGGCC ACAAGAGCTT GATGAAGCTG TTCTCAGGCG TTTTACCAAA 
CGGGTTTATG TGTCTTTGCC AAATGAGGAG ACACGACTAC TTTTATTAAA AAATCTATTA 
TGTAAACAAG GAAGCCCATT GACCCAAAAA GAACTAGCTC AACTTGCTAG AATGACCAAC 
GGATACTCAG GCAGTGATCT AACAGCTTTG GCAAAGGATG CAGCACTGGG TCCTATCCGA 
GAACTGAAAC CAGAACAAGT GAAGAATATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CCGAATCCTT AAAAAAGATA AAACGCAGTG TGAGCCCTCA AACCTTAGAA 
GCATACATAC GTTGGAACAA GGACTTTGGA GATACCACTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005212529.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212586.1 Bos taurus spastin (SPAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23843
Clone ID Related Accession (Same CDS sequence)	XM_005212529.3 , XM_005212529.2
Accession Version	XM_005212529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAGA AGCTGCAACC AAGTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GACAGTACTA ACTTGACATG CCGTAATGGA 
CATCTCCAGT CAGAAAGTGG AGCTGTTCCA AAAAGAAAAG ATCCGTTAAC ACATGCTAGT 
AATTCACTGC CTCGTTCAAA AACAGTTATG AAAACTGGAC CCACAGGTCT TTCAGGCCAC 
CATAGAGCAC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GCGTGAGACA AGGACCTGGT 
TCTGCAGCTG CCACTCATAA GAGTACACCA AAAACAAATA GAACAAATAA GCCTTCTACC 
CCTACAACTG CTGCTCGTAA AAAGAAAGAC TTGAAGAATT TTAGGAATGT GGACAGCAAC 
CTTGCTAACC TTATAATGAA TGAAATTGTG GACAATGGAA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AAGAGCTGGC AAAACAAGCA TTGCAAGAAA TTGTTATTCT TCCTTCTCTG 
AGACCTGAGT TGTTCACAGG GCTTAGGGCT CCTGCCAGAG GACTGTTACT CTTTGGCCCA 
CCTGGAAATG GGAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCAACCTTC 
TTTAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTAT TTGCTGTGGC TCGAGAACTT CAACCTTCCA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAAAG AAGAGAAGGA GAACATGATG CTAGCAGACG TCTAAAAACT 
GAATTTCTAA TAGAATTTGA TGGTGTACAG TCTGCTGGAG ATGACAGAGT GCTTGTAATG 
GGTGCAACCA ACAGGCCACA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT TACCAAACGG 
GTTTATGTGT CTTTGCCAAA TGAGGAGACA CGACTACTTT TATTAAAAAA TCTATTATGT 
AAACAAGGAA GCCCATTGAC CCAAAAAGAA CTAGCTCAAC TTGCTAGAAT GACCAACGGA 
TACTCAGGCA GTGATCTAAC AGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AACAAGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACCG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GCCCTCAAAC CTTAGAAGCA 
TACATACGTT GGAACAAGGA CTTTGGAGAT ACCACTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212586.1
CDS	164..2005
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKLQPSLQFSKSQTDVYNDSTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005212529.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212529.3

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAGA AGCTGCAACC AAGTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GACAGTACTA ACTTGACATG CCGTAATGGA 
CATCTCCAGT CAGAAAGTGG AGCTGTTCCA AAAAGAAAAG ATCCGTTAAC ACATGCTAGT 
AATTCACTGC CTCGTTCAAA AACAGTTATG AAAACTGGAC CCACAGGTCT TTCAGGCCAC 
CATAGAGCAC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GCGTGAGACA AGGACCTGGT 
TCTGCAGCTG CCACTCATAA GAGTACACCA AAAACAAATA GAACAAATAA GCCTTCTACC 
CCTACAACTG CTGCTCGTAA AAAGAAAGAC TTGAAGAATT TTAGGAATGT GGACAGCAAC 
CTTGCTAACC TTATAATGAA TGAAATTGTG GACAATGGAA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AAGAGCTGGC AAAACAAGCA TTGCAAGAAA TTGTTATTCT TCCTTCTCTG 
AGACCTGAGT TGTTCACAGG GCTTAGGGCT CCTGCCAGAG GACTGTTACT CTTTGGCCCA 
CCTGGAAATG GGAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCAACCTTC 
TTTAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTAT TTGCTGTGGC TCGAGAACTT CAACCTTCCA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAAAG AAGAGAAGGA GAACATGATG CTAGCAGACG TCTAAAAACT 
GAATTTCTAA TAGAATTTGA TGGTGTACAG TCTGCTGGAG ATGACAGAGT GCTTGTAATG 
GGTGCAACCA ACAGGCCACA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT TACCAAACGG 
GTTTATGTGT CTTTGCCAAA TGAGGAGACA CGACTACTTT TATTAAAAAA TCTATTATGT 
AAACAAGGAA GCCCATTGAC CCAAAAAGAA CTAGCTCAAC TTGCTAGAAT GACCAACGGA 
TACTCAGGCA GTGATCTAAC AGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AACAAGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACCG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GCCCTCAAAC CTTAGAAGCA 
TACATACGTT GGAACAAGGA CTTTGGAGAT ACCACTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005212529.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212586.1 Bos taurus spastin (SPAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23843
Clone ID Related Accession (Same CDS sequence)	XM_005212529.3 , XM_005212529.2
Accession Version	XM_005212529.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005212529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAGA AGCTGCAACC AAGTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GACAGTACTA ACTTGACATG CCGTAATGGA 
CATCTCCAGT CAGAAAGTGG AGCTGTTCCA AAAAGAAAAG ATCCGTTAAC ACATGCTAGT 
AATTCACTGC CTCGTTCAAA AACAGTTATG AAAACTGGAC CCACAGGTCT TTCAGGCCAC 
CATAGAGCAC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GCGTGAGACA AGGACCTGGT 
TCTGCAGCTG CCACTCATAA GAGTACACCA AAAACAAATA GAACAAATAA GCCTTCTACC 
CCTACAACTG CTGCTCGTAA AAAGAAAGAC TTGAAGAATT TTAGGAATGT GGACAGCAAC 
CTTGCTAACC TTATAATGAA TGAAATTGTG GACAATGGAA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AAGAGCTGGC AAAACAAGCA TTGCAAGAAA TTGTTATTCT TCCTTCTCTG 
AGACCTGAGT TGTTCACAGG GCTTAGGGCT CCTGCCAGAG GACTGTTACT CTTTGGCCCA 
CCTGGAAATG GGAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCAACCTTC 
TTTAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTAT TTGCTGTGGC TCGAGAACTT CAACCTTCCA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAAAG AAGAGAAGGA GAACATGATG CTAGCAGACG TCTAAAAACT 
GAATTTCTAA TAGAATTTGA TGGTGTACAG TCTGCTGGAG ATGACAGAGT GCTTGTAATG 
GGTGCAACCA ACAGGCCACA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT TACCAAACGG 
GTTTATGTGT CTTTGCCAAA TGAGGAGACA CGACTACTTT TATTAAAAAA TCTATTATGT 
AAACAAGGAA GCCCATTGAC CCAAAAAGAA CTAGCTCAAC TTGCTAGAAT GACCAACGGA 
TACTCAGGCA GTGATCTAAC AGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AACAAGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACCG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GCCCTCAAAC CTTAGAAGCA 
TACATACGTT GGAACAAGGA CTTTGGAGAT ACCACTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212586.1
CDS	322..2163
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKLQPSLQFSKSQTDVYNDSTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005212529.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212529.2

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGG ACAAAAGGAC 
CAAGCTGTGG AATGGTATAA GAAAGGTATT GAAGAACTAG AAAAAGGAAT AGCCGTCGTA 
GTTACAGGAC AAGGTGAACA GTGTGAAAGA GCTAGACGTC TTCAGGCTAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTATTAGAGA AGCTGCAACC AAGTTTGCAA 
TTTTCCAAGT CACAAACGGA CGTCTATAAT GACAGTACTA ACTTGACATG CCGTAATGGA 
CATCTCCAGT CAGAAAGTGG AGCTGTTCCA AAAAGAAAAG ATCCGTTAAC ACATGCTAGT 
AATTCACTGC CTCGTTCAAA AACAGTTATG AAAACTGGAC CCACAGGTCT TTCAGGCCAC 
CATAGAGCAC CTAGTTGCAG TGGTTTATCC ATGGTTTCTG GCGTGAGACA AGGACCTGGT 
TCTGCAGCTG CCACTCATAA GAGTACACCA AAAACAAATA GAACAAATAA GCCTTCTACC 
CCTACAACTG CTGCTCGTAA AAAGAAAGAC TTGAAGAATT TTAGGAATGT GGACAGCAAC 
CTTGCTAACC TTATAATGAA TGAAATTGTG GACAATGGAA CAGCTGTTAA ATTTGATGAT 
ATAGCTGGTC AAGAGCTGGC AAAACAAGCA TTGCAAGAAA TTGTTATTCT TCCTTCTCTG 
AGACCTGAGT TGTTCACAGG GCTTAGGGCT CCTGCCAGAG GACTGTTACT CTTTGGCCCA 
CCTGGAAATG GGAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAATCTAA TGCAACCTTC 
TTTAATATAA GTGCTGCAAG TTTAACTTCA AAATATGTAG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTAT TTGCTGTGGC TCGAGAACTT CAACCTTCCA TAATTTTTAT AGATGAAGTT 
GATAGTCTTT TGTGTGAAAG AAGAGAAGGA GAACATGATG CTAGCAGACG TCTAAAAACT 
GAATTTCTAA TAGAATTTGA TGGTGTACAG TCTGCTGGAG ATGACAGAGT GCTTGTAATG 
GGTGCAACCA ACAGGCCACA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT TACCAAACGG 
GTTTATGTGT CTTTGCCAAA TGAGGAGACA CGACTACTTT TATTAAAAAA TCTATTATGT 
AAACAAGGAA GCCCATTGAC CCAAAAAGAA CTAGCTCAAC TTGCTAGAAT GACCAACGGA 
TACTCAGGCA GTGATCTAAC AGCTTTGGCA AAGGATGCAG CACTGGGTCC TATCCGAGAA 
CTGAAACCAG AACAAGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACCG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GCCCTCAAAC CTTAGAAGCA 
TACATACGTT GGAACAAGGA CTTTGGAGAT ACCACTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005212530.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212587.1 Bos taurus spastin (SPAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23844
Clone ID Related Accession (Same CDS sequence)	XM_005212530.3 , XM_005212530.2
Accession Version	XM_005212530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AAAGTGGAGC TGTTCCAAAA 
AGAAAAGATC CGTTAACACA TGCTAGTAAT TCACTGCCTC GTTCAAAAAC AGTTATGAAA 
ACTGGACCCA CAGGTCTTTC AGGCCACCAT AGAGCACCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGCG TGAGACAAGG ACCTGGTTCT GCAGCTGCCA CTCATAAGAG TACACCAAAA 
ACAAATAGAA CAAATAAGCC TTCTACCCCT ACAACTGCTG CTCGTAAAAA GAAAGACTTG 
AAGAATTTTA GGAATGTGGA CAGCAACCTT GCTAACCTTA TAATGAATGA AATTGTGGAC 
AATGGAACAG CTGTTAAATT TGATGATATA GCTGGTCAAG AGCTGGCAAA ACAAGCATTG 
CAAGAAATTG TTATTCTTCC TTCTCTGAGA CCTGAGTTGT TCACAGGGCT TAGGGCTCCT 
GCCAGAGGAC TGTTACTCTT TGGCCCACCT GGAAATGGGA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC AACCTTCTTT AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AAGGAGAGAA ATTGGTGAGA GCTCTATTTG CTGTGGCTCG AGAACTTCAA 
CCTTCCATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAAAGAAG AGAAGGAGAA 
CATGATGCTA GCAGACGTCT AAAAACTGAA TTTCTAATAG AATTTGATGG TGTACAGTCT 
GCTGGAGATG ACAGAGTGCT TGTAATGGGT GCAACCAACA GGCCACAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTTAC CAAACGGGTT TATGTGTCTT TGCCAAATGA GGAGACACGA 
CTACTTTTAT TAAAAAATCT ATTATGTAAA CAAGGAAGCC CATTGACCCA AAAAGAACTA 
GCTCAACTTG CTAGAATGAC CAACGGATAC TCAGGCAGTG ATCTAACAGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAAC AAGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACCGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGCC CTCAAACCTT AGAAGCATAC ATACGTTGGA ACAAGGACTT TGGAGATACC 
ACTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212587.1
CDS	382..2130
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKVGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005212530.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212530.3

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AAAGTGGAGC TGTTCCAAAA 
AGAAAAGATC CGTTAACACA TGCTAGTAAT TCACTGCCTC GTTCAAAAAC AGTTATGAAA 
ACTGGACCCA CAGGTCTTTC AGGCCACCAT AGAGCACCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGCG TGAGACAAGG ACCTGGTTCT GCAGCTGCCA CTCATAAGAG TACACCAAAA 
ACAAATAGAA CAAATAAGCC TTCTACCCCT ACAACTGCTG CTCGTAAAAA GAAAGACTTG 
AAGAATTTTA GGAATGTGGA CAGCAACCTT GCTAACCTTA TAATGAATGA AATTGTGGAC 
AATGGAACAG CTGTTAAATT TGATGATATA GCTGGTCAAG AGCTGGCAAA ACAAGCATTG 
CAAGAAATTG TTATTCTTCC TTCTCTGAGA CCTGAGTTGT TCACAGGGCT TAGGGCTCCT 
GCCAGAGGAC TGTTACTCTT TGGCCCACCT GGAAATGGGA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC AACCTTCTTT AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AAGGAGAGAA ATTGGTGAGA GCTCTATTTG CTGTGGCTCG AGAACTTCAA 
CCTTCCATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAAAGAAG AGAAGGAGAA 
CATGATGCTA GCAGACGTCT AAAAACTGAA TTTCTAATAG AATTTGATGG TGTACAGTCT 
GCTGGAGATG ACAGAGTGCT TGTAATGGGT GCAACCAACA GGCCACAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTTAC CAAACGGGTT TATGTGTCTT TGCCAAATGA GGAGACACGA 
CTACTTTTAT TAAAAAATCT ATTATGTAAA CAAGGAAGCC CATTGACCCA AAAAGAACTA 
GCTCAACTTG CTAGAATGAC CAACGGATAC TCAGGCAGTG ATCTAACAGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAAC AAGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACCGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGCC CTCAAACCTT AGAAGCATAC ATACGTTGGA ACAAGGACTT TGGAGATACC 
ACTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005212530.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212587.1 Bos taurus spastin (SPAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23844
Clone ID Related Accession (Same CDS sequence)	XM_005212530.3 , XM_005212530.2
Accession Version	XM_005212530.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005212530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AAAGTGGAGC TGTTCCAAAA 
AGAAAAGATC CGTTAACACA TGCTAGTAAT TCACTGCCTC GTTCAAAAAC AGTTATGAAA 
ACTGGACCCA CAGGTCTTTC AGGCCACCAT AGAGCACCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGCG TGAGACAAGG ACCTGGTTCT GCAGCTGCCA CTCATAAGAG TACACCAAAA 
ACAAATAGAA CAAATAAGCC TTCTACCCCT ACAACTGCTG CTCGTAAAAA GAAAGACTTG 
AAGAATTTTA GGAATGTGGA CAGCAACCTT GCTAACCTTA TAATGAATGA AATTGTGGAC 
AATGGAACAG CTGTTAAATT TGATGATATA GCTGGTCAAG AGCTGGCAAA ACAAGCATTG 
CAAGAAATTG TTATTCTTCC TTCTCTGAGA CCTGAGTTGT TCACAGGGCT TAGGGCTCCT 
GCCAGAGGAC TGTTACTCTT TGGCCCACCT GGAAATGGGA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC AACCTTCTTT AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AAGGAGAGAA ATTGGTGAGA GCTCTATTTG CTGTGGCTCG AGAACTTCAA 
CCTTCCATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAAAGAAG AGAAGGAGAA 
CATGATGCTA GCAGACGTCT AAAAACTGAA TTTCTAATAG AATTTGATGG TGTACAGTCT 
GCTGGAGATG ACAGAGTGCT TGTAATGGGT GCAACCAACA GGCCACAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTTAC CAAACGGGTT TATGTGTCTT TGCCAAATGA GGAGACACGA 
CTACTTTTAT TAAAAAATCT ATTATGTAAA CAAGGAAGCC CATTGACCCA AAAAGAACTA 
GCTCAACTTG CTAGAATGAC CAACGGATAC TCAGGCAGTG ATCTAACAGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAAC AAGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACCGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGCC CTCAAACCTT AGAAGCATAC ATACGTTGGA ACAAGGACTT TGGAGATACC 
ACTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212587.1
CDS	322..2070
Translation	MNSPGGRGKKKGSGGPSSPVPPRPPPPCQARSRPAPKPAPPPQSPHKRNLYYFSYPLFLGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPASASPPAPVPGGEAERVRAFHKQAFEYISVALRIDEDEKVGQKDQAVEWYKKGIEELEKGIAVVVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVMKTGPTGLSGHHRAPSCSGLSMVSGVRQGPGSAAATHKSTPKTNRTNKPSTPTTAARKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFTKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTNGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005212530.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spastin (SPAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212530.2

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCCCCAG CAGCCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCAGGCC CGCTCCCGTC CCGCCCCCAA GCCGGCCCCT 
CCGCCCCAAT CGCCGCATAA GCGGAACCTG TACTACTTCT CCTACCCGCT GTTCTTAGGC 
TTCGCGCTGC TGCGCCTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCCGCCAAG AGGAGCTCCG GGGCCGCGCC GGCCTCGGCG 
TCGCCCCCGG CACCGGTGCC AGGCGGGGAG GCCGAGCGCG TCCGAGCGTT CCACAAACAG 
GCCTTCGAGT ACATCTCCGT CGCCCTGCGC ATCGATGAGG ACGAGAAAGT AGGACAAAAG 
GACCAAGCTG TGGAATGGTA TAAGAAAGGT ATTGAAGAAC TAGAAAAAGG AATAGCCGTC 
GTAGTTACAG GACAAGGTGA ACAGTGTGAA AGAGCTAGAC GTCTTCAGGC TAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTATTAG AAAGTGGAGC TGTTCCAAAA 
AGAAAAGATC CGTTAACACA TGCTAGTAAT TCACTGCCTC GTTCAAAAAC AGTTATGAAA 
ACTGGACCCA CAGGTCTTTC AGGCCACCAT AGAGCACCTA GTTGCAGTGG TTTATCCATG 
GTTTCTGGCG TGAGACAAGG ACCTGGTTCT GCAGCTGCCA CTCATAAGAG TACACCAAAA 
ACAAATAGAA CAAATAAGCC TTCTACCCCT ACAACTGCTG CTCGTAAAAA GAAAGACTTG 
AAGAATTTTA GGAATGTGGA CAGCAACCTT GCTAACCTTA TAATGAATGA AATTGTGGAC 
AATGGAACAG CTGTTAAATT TGATGATATA GCTGGTCAAG AGCTGGCAAA ACAAGCATTG 
CAAGAAATTG TTATTCTTCC TTCTCTGAGA CCTGAGTTGT TCACAGGGCT TAGGGCTCCT 
GCCAGAGGAC TGTTACTCTT TGGCCCACCT GGAAATGGGA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AATCTAATGC AACCTTCTTT AATATAAGTG CTGCAAGTTT AACTTCAAAA 
TATGTAGGAG AAGGAGAGAA ATTGGTGAGA GCTCTATTTG CTGTGGCTCG AGAACTTCAA 
CCTTCCATAA TTTTTATAGA TGAAGTTGAT AGTCTTTTGT GTGAAAGAAG AGAAGGAGAA 
CATGATGCTA GCAGACGTCT AAAAACTGAA TTTCTAATAG AATTTGATGG TGTACAGTCT 
GCTGGAGATG ACAGAGTGCT TGTAATGGGT GCAACCAACA GGCCACAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTTAC CAAACGGGTT TATGTGTCTT TGCCAAATGA GGAGACACGA 
CTACTTTTAT TAAAAAATCT ATTATGTAAA CAAGGAAGCC CATTGACCCA AAAAGAACTA 
GCTCAACTTG CTAGAATGAC CAACGGATAC TCAGGCAGTG ATCTAACAGC TTTGGCAAAG 
GATGCAGCAC TGGGTCCTAT CCGAGAACTG AAACCAGAAC AAGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACCGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGCC CTCAAACCTT AGAAGCATAC ATACGTTGGA ACAAGGACTT TGGAGATACC 
ACTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Congenital bovine spinal dysmyelination is caused by a missense mutation in the SPAST gene.
Neurogenetics11(2)175-83(2010 May)
Thomsen B,Nissen PH,Agerholm JS,Bendixen C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SPAST cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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