NPHS1 cDNA ORF clone, Bos taurus(cattle)

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225018 XM_024978255.1
Latest version!
Bos taurus NPHS1, nephrin (NPHS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa39809 NM_001192512.1
Latest version!
Bos taurus NPHS1 adhesion molecule, nephrin (NPHS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa28639 XM_005218956.4
Latest version!
Bos taurus NPHS1, nephrin (NPHS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa28639 XM_005218956.3 Bos taurus nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225018
    Clone ID Related Accession (Same CDS sequence) XM_024978255.1
    Accession Version XM_024978255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    GTGAGGGCAG AGGCGGCATG GAGGTGGGGG GAAGGGTGGG GGCCAGGCGC TGGCTCCGAG
    CTGGCCCAGG CCTGTCCGTG TCTCCAGAAT GGCCAGCCCG TGTCCACAGC ATGGGGCACA
    GAACATGCTC AGGCGGTGGC ACGCAGCCTC TTAGTGATGA TCGTGAGGCC GGAAGACCAT
    GGGGCGAGGC TCAGCTGCGA GGCCTACAAC AGTGTATCTT CAGGGATCCA GGAACGTGGG
    GTCACGCTGC AGGTCACCTT CCCCCCCAGT GCCATTACTA TCTTGGGATC TGCGTCCCAG
    TCTGAGAACA AGAACGTGAC ACTCTCCTGC ATCACCAAGT CCAGTCGCCC ACGGGTCCTC
    CTGCGATGGT GGCTGGGCTG GCGGCAGCTG CAGCCCAGCG AGGAGACGGT CATGGACGGC
    CTGCATGGTG GCCACATCTC CATGTCTAAC CTGACATTCC TGGCGCGACG GGAGGACAAC
    GGTCTGACCC TCACGTGTGA GGCCTTTAGC GAGGCTTTCA CCAAAGAGAC CTTCAAGAAG
    TCGCTTACCC TAAATGTGAA ATATCCTGCC CAGAAGCTGT GGATTGAAGG CCCTCCAGAG
    GGGCAGCGCC TCCGGGCTGG GAACCGGGTG AGGCTGGTGT GTTTGGCCAT TGGAGGCAAC
    CCAGACCCTT CCCTCACCTG GTACAAGGAC TCGCGCACCG TGACCGAACC GCGGCCGCCC
    CAGGAACCAC GGCGGGTGCA GCTGGGAAGT GTGGAGAAGT CGGGGAGCAT CTTCTCCCGA
    GAGCTGGTGC TGATCACGGG TCCGTCGGAC AACCAGGCCA AGTTCACGTG CAAAGCCGGC
    CAGCTCAGCG CGTCCACGCA GCTCGTGGTG CAGTTCCCCC CGACAAATGT GACGATCCTG
    GCCAACGCGT CGACGCTACG CCCCGGGGAT GCCTTAAACT TGACGTGCGT CAGCGTTAGC
    AGCAACCCTC CAGTCAACTT GTCGTGGGAC AAGGAGGGGG AGAGGCTGGA AGATGTGGCC
    GCCCAGCCCC GCCGGGCTCC ATTCAAAGGT TCCGCCGCCG CTAGGAGCGT CCTTCTGAGA
    CTCTCATCCC GTGACCACGG CCACCGCGTG ACCTGTAGCG CCCACAGCAC CGAGCTGCGG
    GAAACCGTGA GCGCCTTCTA CCGCCTCAAC GTGCTGTACC CTCCAGAGTT CCTGGGGGAG
    CAGGTGCTTG TGGTGACCGC GGTGGAGCAG GGCGAGGCAC TGCTGCCCGT CTCCGTGTCT
    GCCAACCCCG CCCCGGAGGC CTTCAACTGG ACCTTCCGCG GCTACCGACT CAGCCCAGCT
    GGCGGCCCTC GGCATCGTAT CCTGTCTGGT GGAGCGCTGC AGCTATGGAA TGTGACCCGC
    GCTGACGATG GCCTTTACCA GTTGCACTGT CAGAACTCGG AGGGCACGGC GGAAGCTCTC
    GTGAGACTGG ATGTACAATA TGCTCCCACC ATCCGAGCTC TCCAAGACCC CACTGAGGTG
    AATGTTGGGG ATTCTGTGGA CATAGTCTGC ACTGTGGATG CCAATCCCAT CCTTCCTGAG
    ATGTTCAACT GGGAGAGGCT GGGAGAAGAA GGGGAGGACC AGAATCTGGA TGACATGGAG
    AAGATGTCAA AGGGATCCAC AGGGCGTCTT CGTATTCACC ATGCCAAGCT GACCCAGGCT
    GGTGCTTACC AATGCATTGT GGACAATGGG GTGGCACCTC CAGCCCGAGG GCTGGTCCGT
    CTTGTTGTCA GATTTGCCCC CCATGTGGAG CATCCCGCTC CCCTAACGAA AGTGGCTGCA
    GCTGGGGACA GCACTAGTTC TGCCACCCTT CACTGTCGTG CCCGAGGCGT CCCCAACATC
    GTCTTCACTT GGACCAAAAA CGGGGTCCCT CTGGATCTCC AGGATCCAAG GTACACAGAG
    CACACATATC ACCAGGGTGG CGTCCACAGC AGCCTTCTGA CCATTGCCAA CGTGTCTGCA
    GCCCAGGACT ATGCCCTCTT CACATGCATA GCCACCAACC CCCTCGGCTC GGACCACACC
    AACATTCAAC TCGTCAGCAT CAGCCGCCCT GATCCGCCAT CGGGACTGAA GGCTGTGAGC
    ATGACCCCAT ATTCAGTGGG CCTGGAGTGG AAGCCTGGCT TTGATGGGGG CTTGCCACAG
    AGGTTCCAAA TCAGGTATGA GGCTCTGGAG ACTCCAGGGT TCCTCTACAT GGATGTCCTA
    CCACCCCAGG CCACCACCTT CACACTGACC GGGCTGCAGC CTTCTACACG ATACAGGGTC
    TGGTTGCTGG CCAGCAATGC CCTGGGTGAC AGCGGACTAG CTGACAAAGT GTCCCAGATC
    TCGATCACGA CCCCAGGTCT AGACCAACCT TCTCGAGAAC CCGACTACCA GCTACCCACA
    GAGCAGCCTG CAGGACCCTC GGAGATGCCC TTGCTGCCTG TGCTGCTTGC TGTTGGAGGT
    CTTTTGCTGC TTTCAAATGC CTCCTGTGTC GGGGGATTCC TCTGGCAGCG GAGACTGAAG
    CGTCTTGCTG AGGGCATTTC CGAGAAGACA GAGACAGGGT CGGAGGAAGA CCGAGTCAGG
    AATGAATATG AGGAGAGCCA GTGGACTGGA GATCGGGACA CTCGAAGCTC CACCGTTAGC
    ACAACAGATG TAGAGCCATA TTACCACTCC ATGAGGGACT TCAGCCCCCA GCTTCCCCCC
    ACGATGGAGG AGGTGTCTTA TCCCCTAGGT CTCAGGGCTC TTGAGGAGGA GAATATGGCC
    TTTCCTGGGC ACCTGTATGA TGAGGTGGAA AGAGTGTATG CTCCATCTCG GGCCTGGGGA
    CCCCTCTACG ATGAAGTACA AATGGACTCC TGTGATTTCC GCTGGCCTGG AGACAGATAT
    GAGGATGCAA GAGGAATTTA TGATCAGGTG GCAGGAGATC TGGACCCTGT GGAACCTGAT
    GCTATACCCT TTGAGCTGAG GGGACATCTG GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834023.1
    CDS112..3927
    Translation

    Target ORF information:

    RefSeq Version XM_024978255.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NPHS1, nephrin (NPHS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    GTGAGGGCAG AGGCGGCATG GAGGTGGGGG GAAGGGTGGG GGCCAGGCGC TGGCTCCGAG
    CTGGCCCAGG CCTGTCCGTG TCTCCAGAAT GGCCAGCCCG TGTCCACAGC ATGGGGCACA
    GAACATGCTC AGGCGGTGGC ACGCAGCCTC TTAGTGATGA TCGTGAGGCC GGAAGACCAT
    GGGGCGAGGC TCAGCTGCGA GGCCTACAAC AGTGTATCTT CAGGGATCCA GGAACGTGGG
    GTCACGCTGC AGGTCACCTT CCCCCCCAGT GCCATTACTA TCTTGGGATC TGCGTCCCAG
    TCTGAGAACA AGAACGTGAC ACTCTCCTGC ATCACCAAGT CCAGTCGCCC ACGGGTCCTC
    CTGCGATGGT GGCTGGGCTG GCGGCAGCTG CAGCCCAGCG AGGAGACGGT CATGGACGGC
    CTGCATGGTG GCCACATCTC CATGTCTAAC CTGACATTCC TGGCGCGACG GGAGGACAAC
    GGTCTGACCC TCACGTGTGA GGCCTTTAGC GAGGCTTTCA CCAAAGAGAC CTTCAAGAAG
    TCGCTTACCC TAAATGTGAA ATATCCTGCC CAGAAGCTGT GGATTGAAGG CCCTCCAGAG
    GGGCAGCGCC TCCGGGCTGG GAACCGGGTG AGGCTGGTGT GTTTGGCCAT TGGAGGCAAC
    CCAGACCCTT CCCTCACCTG GTACAAGGAC TCGCGCACCG TGACCGAACC GCGGCCGCCC
    CAGGAACCAC GGCGGGTGCA GCTGGGAAGT GTGGAGAAGT CGGGGAGCAT CTTCTCCCGA
    GAGCTGGTGC TGATCACGGG TCCGTCGGAC AACCAGGCCA AGTTCACGTG CAAAGCCGGC
    CAGCTCAGCG CGTCCACGCA GCTCGTGGTG CAGTTCCCCC CGACAAATGT GACGATCCTG
    GCCAACGCGT CGACGCTACG CCCCGGGGAT GCCTTAAACT TGACGTGCGT CAGCGTTAGC
    AGCAACCCTC CAGTCAACTT GTCGTGGGAC AAGGAGGGGG AGAGGCTGGA AGATGTGGCC
    GCCCAGCCCC GCCGGGCTCC ATTCAAAGGT TCCGCCGCCG CTAGGAGCGT CCTTCTGAGA
    CTCTCATCCC GTGACCACGG CCACCGCGTG ACCTGTAGCG CCCACAGCAC CGAGCTGCGG
    GAAACCGTGA GCGCCTTCTA CCGCCTCAAC GTGCTGTACC CTCCAGAGTT CCTGGGGGAG
    CAGGTGCTTG TGGTGACCGC GGTGGAGCAG GGCGAGGCAC TGCTGCCCGT CTCCGTGTCT
    GCCAACCCCG CCCCGGAGGC CTTCAACTGG ACCTTCCGCG GCTACCGACT CAGCCCAGCT
    GGCGGCCCTC GGCATCGTAT CCTGTCTGGT GGAGCGCTGC AGCTATGGAA TGTGACCCGC
    GCTGACGATG GCCTTTACCA GTTGCACTGT CAGAACTCGG AGGGCACGGC GGAAGCTCTC
    GTGAGACTGG ATGTACAATA TGCTCCCACC ATCCGAGCTC TCCAAGACCC CACTGAGGTG
    AATGTTGGGG ATTCTGTGGA CATAGTCTGC ACTGTGGATG CCAATCCCAT CCTTCCTGAG
    ATGTTCAACT GGGAGAGGCT GGGAGAAGAA GGGGAGGACC AGAATCTGGA TGACATGGAG
    AAGATGTCAA AGGGATCCAC AGGGCGTCTT CGTATTCACC ATGCCAAGCT GACCCAGGCT
    GGTGCTTACC AATGCATTGT GGACAATGGG GTGGCACCTC CAGCCCGAGG GCTGGTCCGT
    CTTGTTGTCA GATTTGCCCC CCATGTGGAG CATCCCGCTC CCCTAACGAA AGTGGCTGCA
    GCTGGGGACA GCACTAGTTC TGCCACCCTT CACTGTCGTG CCCGAGGCGT CCCCAACATC
    GTCTTCACTT GGACCAAAAA CGGGGTCCCT CTGGATCTCC AGGATCCAAG GTACACAGAG
    CACACATATC ACCAGGGTGG CGTCCACAGC AGCCTTCTGA CCATTGCCAA CGTGTCTGCA
    GCCCAGGACT ATGCCCTCTT CACATGCATA GCCACCAACC CCCTCGGCTC GGACCACACC
    AACATTCAAC TCGTCAGCAT CAGCCGCCCT GATCCGCCAT CGGGACTGAA GGCTGTGAGC
    ATGACCCCAT ATTCAGTGGG CCTGGAGTGG AAGCCTGGCT TTGATGGGGG CTTGCCACAG
    AGGTTCCAAA TCAGGTATGA GGCTCTGGAG ACTCCAGGGT TCCTCTACAT GGATGTCCTA
    CCACCCCAGG CCACCACCTT CACACTGACC GGGCTGCAGC CTTCTACACG ATACAGGGTC
    TGGTTGCTGG CCAGCAATGC CCTGGGTGAC AGCGGACTAG CTGACAAAGT GTCCCAGATC
    TCGATCACGA CCCCAGGTCT AGACCAACCT TCTCGAGAAC CCGACTACCA GCTACCCACA
    GAGCAGCCTG CAGGACCCTC GGAGATGCCC TTGCTGCCTG TGCTGCTTGC TGTTGGAGGT
    CTTTTGCTGC TTTCAAATGC CTCCTGTGTC GGGGGATTCC TCTGGCAGCG GAGACTGAAG
    CGTCTTGCTG AGGGCATTTC CGAGAAGACA GAGACAGGGT CGGAGGAAGA CCGAGTCAGG
    AATGAATATG AGGAGAGCCA GTGGACTGGA GATCGGGACA CTCGAAGCTC CACCGTTAGC
    ACAACAGATG TAGAGCCATA TTACCACTCC ATGAGGGACT TCAGCCCCCA GCTTCCCCCC
    ACGATGGAGG AGGTGTCTTA TCCCCTAGGT CTCAGGGCTC TTGAGGAGGA GAATATGGCC
    TTTCCTGGGC ACCTGTATGA TGAGGTGGAA AGAGTGTATG CTCCATCTCG GGCCTGGGGA
    CCCCTCTACG ATGAAGTACA AATGGACTCC TGTGATTTCC GCTGGCCTGG AGACAGATAT
    GAGGATGCAA GAGGAATTTA TGATCAGGTG GCAGGAGATC TGGACCCTGT GGAACCTGAT
    GCTATACCCT TTGAGCTGAG GGGACATCTG GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39809
    Clone ID Related Accession (Same CDS sequence) NM_001192512.1
    Accession Version NM_001192512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-29
    Organism Bos taurus(cattle)
    Product nephrin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On Jul 17, 2010 this sequence version replaced XM_597931.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145414, SAMN03145419 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTGAGGCTC TTGAGGAGGA GAATATGGCC TTTCCTGGGC ACCTGTATGA TGAGGTGGAA
    AGAGTGTATG CTCCATCTCG GGCCTGGGGA CCCCTCTACG ATGAAGTACA AATGGACTCC
    TGTGATTTCC GCTGGCCTGG AGACAGATAT GAGGATGCAA GAGGAATTTA TGATCAGGTG
    GCAGGAGATC TGGACCCTGT GGAACCTGAT GCTATACCCT TTGAGCTGAG GGGACATCTG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179441.1
    CDS1..3726
    Translation

    Target ORF information:

    RefSeq Version NM_001192512.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NPHS1 adhesion molecule, nephrin (NPHS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTGAGGCTC TTGAGGAGGA GAATATGGCC TTTCCTGGGC ACCTGTATGA TGAGGTGGAA
    AGAGTGTATG CTCCATCTCG GGCCTGGGGA CCCCTCTACG ATGAAGTACA AATGGACTCC
    TGTGATTTCC GCTGGCCTGG AGACAGATAT GAGGATGCAA GAGGAATTTA TGATCAGGTG
    GCAGGAGATC TGGACCCTGT GGAACCTGAT GCTATACCCT TTGAGCTGAG GGGACATCTG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28639
    Clone ID Related Accession (Same CDS sequence) XM_005218956.3 , XM_005218956.4
    Accession Version XM_005218956.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTCTCAGGG CTCTTGAGGA GGAGAATATG GCCTTTCCTG GGCACCTGTA TGATGAGGTG
    GAAAGAGTGT ATGCTCCATC TCGGGCCTGG GGACCCCTCT ACGATGAAGT ACAAATGGAC
    TCCTGTGATT TCCGCTGGCC TGGAGACAGA TATGAGGATG CAAGAGGAAT TTATGATCAG
    GTGGCAGGAG ATCTGGACCC TGTGGAACCT GATGCTATAC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219013.1
    CDS910..4638
    Translation

    Target ORF information:

    RefSeq Version XM_005218956.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTCTCAGGG CTCTTGAGGA GGAGAATATG GCCTTTCCTG GGCACCTGTA TGATGAGGTG
    GAAAGAGTGT ATGCTCCATC TCGGGCCTGG GGACCCCTCT ACGATGAAGT ACAAATGGAC
    TCCTGTGATT TCCGCTGGCC TGGAGACAGA TATGAGGATG CAAGAGGAAT TTATGATCAG
    GTGGCAGGAG ATCTGGACCC TGTGGAACCT GATGCTATAC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28639
    Clone ID Related Accession (Same CDS sequence) XM_005218956.3 , XM_005218956.4
    Accession Version XM_005218956.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nephrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218956.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTCTCAGGG CTCTTGAGGA GGAGAATATG GCCTTTCCTG GGCACCTGTA TGATGAGGTG
    GAAAGAGTGT ATGCTCCATC TCGGGCCTGG GGACCCCTCT ACGATGAAGT ACAAATGGAC
    TCCTGTGATT TCCGCTGGCC TGGAGACAGA TATGAGGATG CAAGAGGAAT TTATGATCAG
    GTGGCAGGAG ATCTGGACCC TGTGGAACCT GATGCTATAC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219013.1
    CDS112..3840
    Translation

    Target ORF information:

    RefSeq Version XM_005218956.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NPHS1, nephrin (NPHS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218956.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCTGA GCACCCACTT CAAGGCTGCA CTACTGCTCC TGGGGCTTCT GATGACAGGC 
    CTGACCCAGG TGCCAATCCT GGCCTCAGCC CCCCGAGGCT TCTGGGCTCT GCCCGAAAAC
    TTGACAGTGG TGGAGGGGGC TGTTGCTGAA CTGCGGTGCG GGGTCCGCGC CCCTGGCAGC
    GCGGTGCAAT GGGCCAAAGA CGGGCTGCTC CTCGGCCCCG ATCCTAGGAT CCCCAGCTTC
    CCGCGGTATC GCCTGGAGGG AGACCCTGCT AAAGGTGAAT TCCACCTGCA TATTGAAGCG
    TGTGACCTCA GCGATGATGC AGAGTATGAG TGCCAGGTCG GCCGCTCAGA GACAGGCCCT
    GAGCTCGTGT CTCCCAGAGT GATCCTCTCC ATCCTGGTGC CCCCCAAGGT GCTTCAGCTG
    ACCCCTGAAG CAGGGAGCAC AGTCACATGG GTAGCTGGGC AGGAGTACAC GGTCAGCTGT
    GTGTCTGGGG ACGCCAAGCC AGCACCTGAC ATCACCTTTC TCTTGAGTGG ACAAACAATT
    TCCGGTATCT CTGCTAATGT GAATGAGGGG TCCCAGCAGA AACTCTTCAC CACGGAGGCC
    ACAGCCAGGG TGACACCCCA GAGCTCGGAT AATGGGCAGT TACTGGTCTG TGAAGGATCC
    AGCCCAGCTT TGGATACCCC TATCAAGGCT TCATTCACCA TGAATGTTCT GTTCCCCCCA
    GGGCCGCCCG TCATAGAATG GCCAGGCCTG GATGAGGGGC AGGTGCGGGC AGGGCAGAGC
    CTGGAGCTGT TGTGCACGGC CCGAGGGGGG AATCCGCTAG CCACACTGCA GTGGCTGAAG
    AATGGCCAGC CCGTGTCCAC AGCATGGGGC ACAGAACATG CTCAGGCGGT GGCACGCAGC
    CTCTTAGTGA TGATCGTGAG GCCGGAAGAC CATGGGGCGA GGCTCAGCTG CGAGGCCTAC
    AACAGTGTAT CTTCAGGGAT CCAGGAACGT GGGGTCACGC TGCAGGTCAC CTTCCCCCCC
    AGTGCCATTA CTATCTTGGG ATCTGCGTCC CAGTCTGAGA ACAAGAACGT GACACTCTCC
    TGCATCACCA AGTCCAGTCG CCCACGGGTC CTCCTGCGAT GGTGGCTGGG CTGGCGGCAG
    CTGCAGCCCA GCGAGGAGAC GGTCATGGAC GGCCTGCATG GTGGCCACAT CTCCATGTCT
    AACCTGACAT TCCTGGCGCG ACGGGAGGAC AACGGTCTGA CCCTCACGTG TGAGGCCTTT
    AGCGAGGCTT TCACCAAAGA GACCTTCAAG AAGTCGCTTA CCCTAAATGT GAAATATCCT
    GCCCAGAAGC TGTGGATTGA AGGCCCTCCA GAGGGGCAGC GCCTCCGGGC TGGGAACCGG
    GTGAGGCTGG TGTGTTTGGC CATTGGAGGC AACCCAGACC CTTCCCTCAC CTGGTACAAG
    GACTCGCGCA CCGTGACCGA ACCGCGGCCG CCCCAGGAAC CACGGCGGGT GCAGCTGGGA
    AGTGTGGAGA AGTCGGGGAG CATCTTCTCC CGAGAGCTGG TGCTGATCAC GGGTCCGTCG
    GACAACCAGG CCAAGTTCAC GTGCAAAGCC GGCCAGCTCA GCGCGTCCAC GCAGCTCGTG
    GTGCAGTTCC CCCCGACAAA TGTGACGATC CTGGCCAACG CGTCGACGCT ACGCCCCGGG
    GATGCCTTAA ACTTGACGTG CGTCAGCGTT AGCAGCAACC CTCCAGTCAA CTTGTCGTGG
    GACAAGGAGG GGGAGAGGCT GGAAGATGTG GCCGCCCAGC CCCGCCGGGC TCCATTCAAA
    GGTTCCGCCG CCGCTAGGAG CGTCCTTCTG AGACTCTCAT CCCGTGACCA CGGCCACCGC
    GTGACCTGTA GCGCCCACAG CACCGAGCTG CGGGAAACCG TGAGCGCCTT CTACCGCCTC
    AACGTGCTGT ACCCTCCAGA GTTCCTGGGG GAGCAGGTGC TTGTGGTGAC CGCGGTGGAG
    CAGGGCGAGG CACTGCTGCC CGTCTCCGTG TCTGCCAACC CCGCCCCGGA GGCCTTCAAC
    TGGACCTTCC GCGGCTACCG ACTCAGCCCA GCTGGCGGCC CTCGGCATCG TATCCTGTCT
    GGTGGAGCGC TGCAGCTATG GAATGTGACC CGCGCTGACG ATGGCCTTTA CCAGTTGCAC
    TGTCAGAACT CGGAGGGCAC GGCGGAAGCT CTCGTGAGAC TGGATGTACA ATATGCTCCC
    ACCATCCGAG CTCTCCAAGA CCCCACTGAG GTGAATGTTG GGGATTCTGT GGACATAGTC
    TGCACTGTGG ATGCCAATCC CATCCTTCCT GAGATGTTCA ACTGGGAGAG GCTGGGAGAA
    GAAGGGGAGG ACCAGAATCT GGATGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTTCGTATTC ACCATGCCAA GCTGACCCAG GCTGGTGCTT ACCAATGCAT TGTGGACAAT
    GGGGTGGCAC CTCCAGCCCG AGGGCTGGTC CGTCTTGTTG TCAGATTTGC CCCCCATGTG
    GAGCATCCCG CTCCCCTAAC GAAAGTGGCT GCAGCTGGGG ACAGCACTAG TTCTGCCACC
    CTTCACTGTC GTGCCCGAGG CGTCCCCAAC ATCGTCTTCA CTTGGACCAA AAACGGGGTC
    CCTCTGGATC TCCAGGATCC AAGGTACACA GAGCACACAT ATCACCAGGG TGGCGTCCAC
    AGCAGCCTTC TGACCATTGC CAACGTGTCT GCAGCCCAGG ACTATGCCCT CTTCACATGC
    ATAGCCACCA ACCCCCTCGG CTCGGACCAC ACCAACATTC AACTCGTCAG CATCAGCCGC
    CCTGATCCGC CATCGGGACT GAAGGCTGTG AGCATGACCC CATATTCAGT GGGCCTGGAG
    TGGAAGCCTG GCTTTGATGG GGGCTTGCCA CAGAGGTTCC AAATCAGGTA TGAGGCTCTG
    GAGACTCCAG GGTTCCTCTA CATGGATGTC CTACCACCCC AGGCCACCAC CTTCACACTG
    ACCGGGCTGC AGCCTTCTAC ACGATACAGG GTCTGGTTGC TGGCCAGCAA TGCCCTGGGT
    GACAGCGGAC TAGCTGACAA AGTGTCCCAG ATCTCGATCA CGACCCCAGG TCTAGACCAA
    CCTTCTCGAG AACCCGACTA CCAGCTACCC ACAGAGCAGC CTGCAGGACC CTCGGAGATG
    CCCTTGCTGC CTGTGCTGCT TGCTGTTGGA GGTCTTTTGC TGCTTTCAAA TGCCTCCTGT
    GTCGGGGGAT TCCTCTGGCA GCGGAGACTG AAGCGTCTTG CTGAGGGCAT TTCCGAGAAG
    ACAGAGACAG GGTCGGAGGA AGACCGAGTC AGGAATGAAT ATGAGGAGAG CCAGTGGACT
    GGAGATCGGG ACACTCGAAG CTCCACCGTT AGCACAACAG ATGTAGAGCC ATATTACCAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCCACGATGG AGGAGGTGTC TTATCCCCTA
    GGTCTCAGGG CTCTTGAGGA GGAGAATATG GCCTTTCCTG GGCACCTGTA TGATGAGGTG
    GAAAGAGTGT ATGCTCCATC TCGGGCCTGG GGACCCCTCT ACGATGAAGT ACAAATGGAC
    TCCTGTGATT TCCGCTGGCC TGGAGACAGA TATGAGGATG CAAGAGGAAT TTATGATCAG
    GTGGCAGGAG ATCTGGACCC TGTGGAACCT GATGCTATAC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.