ZNF516 cDNA ORF clone, Bos taurus(cattle)

The following ZNF516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa32364 XM_002697653.5
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32364 XM_024984595.1
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32364 XM_597668.6
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32364 XM_024984594.1
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32365 XM_005223945.3
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32365 XM_005196716.3
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32365 XM_024984596.1
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32366 XM_010818662.2
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32366 XM_024984597.1
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32366 XM_010825518.2
Latest version!
Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OBa32364 XM_002697653.4 Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa32364
    Clone ID Related Accession (Same CDS sequence) XM_597668.6 , XM_002697653.5 , XM_024984594.1 , XM_002697653.4 , XM_024984595.1
    Accession Version XM_597668.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646631.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_597668.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_597668.4
    CDS320..3646
    Translation

    Target ORF information:

    RefSeq Version XM_597668.6
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_597668.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32364
    Clone ID Related Accession (Same CDS sequence) XM_597668.6 , XM_002697653.5 , XM_024984594.1 , XM_002697653.4 , XM_024984595.1
    Accession Version XM_002697653.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697653.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697699.2
    CDS309..3635
    Translation

    Target ORF information:

    RefSeq Version XM_002697653.5
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697653.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32364
    Clone ID Related Accession (Same CDS sequence) XM_597668.6 , XM_002697653.5 , XM_024984594.1 , XM_002697653.4 , XM_024984595.1
    Accession Version XM_024984594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840362.1
    CDS270..3596
    Translation

    Target ORF information:

    RefSeq Version XM_024984594.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32364
    Clone ID Related Accession (Same CDS sequence) XM_597668.6 , XM_002697653.5 , XM_024984594.1 , XM_002697653.4 , XM_024984595.1
    Accession Version XM_002697653.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104565.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697653.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697699.2
    CDS320..3646
    Translation

    Target ORF information:

    RefSeq Version XM_002697653.4
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697653.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32364
    Clone ID Related Accession (Same CDS sequence) XM_597668.6 , XM_002697653.5 , XM_024984594.1 , XM_002697653.4 , XM_024984595.1
    Accession Version XM_024984595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840363.1
    CDS304..3630
    Translation

    Target ORF information:

    RefSeq Version XM_024984595.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGT GCTGTTCGAG TCTAATGGCC TCCGAGGTGC TGACGCCCAC
    ACCACCTCCG CAGACACCCC CAAACAAGGG AGAGACCATT CTAACGCAGA TGCTGTCCAA
    ACAGTGCCTC TCAGAAAGGG AACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32365
    Clone ID Related Accession (Same CDS sequence) XM_005223945.3 , XM_024984596.1 , XM_005196716.3
    Accession Version XM_005223945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104565.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224002.1
    CDS320..3535
    Translation

    Target ORF information:

    RefSeq Version XM_005223945.3
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32365
    Clone ID Related Accession (Same CDS sequence) XM_005223945.3 , XM_024984596.1 , XM_005196716.3
    Accession Version XM_024984596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840364.1
    CDS270..3485
    Translation

    Target ORF information:

    RefSeq Version XM_024984596.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32365
    Clone ID Related Accession (Same CDS sequence) XM_005223945.3 , XM_024984596.1 , XM_005196716.3
    Accession Version XM_005196716.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646631.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196773.1
    CDS320..3535
    Translation

    Target ORF information:

    RefSeq Version XM_005196716.3
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196716.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGACGC TCCGGACGCA GGCCCACCAG
    GGAGACTTCA TCTGCGTGGA GTGTGGGAAG AGCTTCCACC AGCCCAGCCA CCTGCGGGCG
    CACATGCGGG CTCACACAGG GGAGAGACCA TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32366
    Clone ID Related Accession (Same CDS sequence) XM_010818662.2 , XM_010825518.2 , XM_024984597.1
    Accession Version XM_010818662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104565.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816964.1
    CDS320..3430
    Translation

    Target ORF information:

    RefSeq Version XM_010818662.2
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32366
    Clone ID Related Accession (Same CDS sequence) XM_010818662.2 , XM_010825518.2 , XM_024984597.1
    Accession Version XM_010825518.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646631.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010823820.1
    CDS320..3430
    Translation

    Target ORF information:

    RefSeq Version XM_010825518.2
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010825518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32366
    Clone ID Related Accession (Same CDS sequence) XM_010818662.2 , XM_010825518.2 , XM_024984597.1
    Accession Version XM_024984597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 516 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840365.1
    CDS270..3380
    Translation

    Target ORF information:

    RefSeq Version XM_024984597.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 516 (ZNF516), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCGCT CCCGGGAGGC CGAGGCGGAG CTGAGAGGGG GCCGCAGCCC GTCCAGGGCC 
    AGCCGGAGCC CCGAGGCGGA CGGGGACAGG GCGCTGAGCC ACAGCTGCTG TATCTGCGGC
    AAGACCTTCC CCTTCCAGAG CTCGCTGTCC CAGCACATGC GCAAGCACAC GGGCGAGAAG
    CCCTACAAGT GCCCCTACTG CGACCACCGC GCCGCGCAGA AGGGCAACCT CAAGATCCAC
    ATCCGCGGGC ACCGCGCGGG CACGCTGACC CAGGGCCGCG AGCCTGAGGC GGCTGAGACG
    CGCGTGTCCG AGGGGCTCGG CGGGTGCACC AGCCCCACCA AGAGCACGTC GGCCTGTAAC
    CGCATCCTGA ACGGCGCCAC GCAGGCGGAC ACCGGCAAGA TCCTGCTGCG CAGCACGCGG
    AAGGAGGCGG AGGGCGTGGC GGCGGGGCAG TGCGCCTTCT GCAAGAGCAG ATTCGAGCGC
    AAGAAGGACC TGGCGCAGCA CGTGCAGCAG GCGCACAAGC CCTTCACGTG CCGCCTGTGC
    AGCTACGCCA CCCTGCGCGA GGAGGCCCTG CTCAGCCACG TGGAGAAGGA TCACATCGCG
    GCGCAGGGGC CGCGCGGAGA CGCTTTCGCC GAGAACGGCA AGCCGGAGCT GCCGCCCGGC
    GAGTTCCCCT GCGAGGTGTG CGGCCAGGCC TTCAGCCAGA CCTGGTTCCT GAAGGCGCAC
    ATGAAGAAGC ACAGGGGCTC CTTTGACCAC GGCTGCCACA TCTGTGGTCG GAGATTCAAG
    GAGCCGTGGT TCCTGAAAAA CCACATGAAG GCGCATGGCC CAAAGGCGGG GAGCAAAAAC
    AGGCCCCGGA GCGAGCTGGA GCCCGTGGCC ACCATCAACG ACGTGGTGCA GGAAGAGGTG
    ATTGTGGCCG GCCTGAGCCT CTATGAAGTC TGCACCAAGT GTGGGAACCT GTTTACGAAC
    CTGGACAGCT TGAACGCGCA CAACGCCATC CACCGCCGGG TGGAGGCCGG CCAACCACGG
    GCCGCCGTCG GGGAGGGCTC AGCGCCCGGC CCCGCCGATT CCCAGCAGTT CTTCCTTCAG
    TGCCTGAACC TGCGCCCGGC GGCCGGCACG CCCACCCGCG GGCAGACTGG GCGGCGCGTG
    GCTGAGCTGG ACCCGGTCAA CAGCTACCAG GCCTGGCAGC TGGCCACCCG GGGCAAGGTG
    GCCGAGCCGG CCGAGTACCT CAAGTACGCG GCGTGGGACG AGGCGCTGGC CGGGGACGTG
    GCCTTCGACA AGGAGCGGCG CGAGTTCATC CTGGTGAGCC AGGAGAAGCG CAAGCGCGAG
    CCTGAACCGG GTGCACAGGG CCCCCCGCGC AAGAGGGCAG GCGCACCCGG GGACCCCGCG
    CCTGGGCCCC CGGATCCCCG GCCCGCGGCA CGCCCCAGCC GCCGTGCTGC CGTCCCTGCC
    GGCCACGGCA AGTCGTCTGA GTGCTTCGAG TGCGGCAAGA TCTTCCGCAC GTACCACCAG
    ATGGTGCTGC ATTCGCGAGT GCACCGCCGC GCGCGCCGCG ACCGAGAGCG CGACGGCCCC
    GGGGACCGTG CGCCGCGTGC CCGCTGCGGC TCCCTCAGCG AGGGCGACTC GGCCTCTCAG
    CCCAGCAGCC CTGGGTCGGG TTGCGCCGCC GCGGACTCCC CGGGCTCGGG CCTCGCTGAT
    GAGGCTGCCG ATGAGAGCGG AGAGGAGGCC CCGGCCCATC CCACAGCAGG AGCAAAGCCA
    CACCGTTGCT GCTTCCCAGA AGACGTCTCC TTGACCGTGC TCTCCAATGG AGACCAAAGC
    CACACACTTG GAAACAGCCC ATCAGGAAGA GCGGCCGGGG AGCCCGGCGC GGGGATCGCG
    GCCTGCGTGT CCATACTTGA AAGCAGCAGC AGGGACGCTC CCAGGAGACC CGAGCCGCCT
    CGGTGTTCTG TGGACTTGAA GACGCCAGCA TGTGGCCCCC AGCAGGAGGG CCCGGGCCCC
    AGGGACGCGG CTGACCTCCC TCCACGCCCG GCCCAGGCGG CGGGTCTGCA GCCGCCCTCT
    GAGAGCCAGG CCGCCTACCC CCGGGAGAGG CCGCCGGATT TGCACACCAA GGAGCACCCC
    ACAGCGGGGA AGCAGGCGCC GGCCCCAGAC CTGGTCCCAT TGGACCTGAG CGAACGGTCC
    TCGCGGGACG ACCCCGGCCG CCGGGAGCCG GCCTCCTCGC TGCAGGCGGC GCTGGCCGTG
    CACCCGTGTC CGTACTGCGG CCATAAGACC TACTATCCCG AGGTGCTCTG GATGCACAAG
    CGTGTCTGGC ACCGGGTCAG CGGCCAGGCC GTGGCCCCGC AGTGGACTCA GCCCAACGGC
    TACAAGAGCA TCAGGAGCAA CCTGGTGTTC CTGGCCCGGA GCGGACGCAC GGGCCCCCCG
    CCTGCCCTCG GGGGCAAGGA GTGCCAGCCT CTGCCCATCG CCCGGTTCAC CCGCACCCAG
    GTGCCGGGCG GGGCGCCAGG CCCCAAAGGC AGCCCCTCGC CCCTGGGGGT GCCCACGAAA
    GCCGCCGGCC TGCCCAGGAG CAAGGAGGGC CACCCTGGGG CGCCCTGTAC ACTCTGGACG
    GCCGGCCCCG ACGGGACCCG GCAGACCCGG CCTGGCCCCA GCCCGGAGCA GCCTCCGCCC
    AAGCCCAAAC AGGAGGCCAG CCCCAGACCC GGGGCCACGG GAGGCAGTGG GGGCTTCAGC
    AGAAGCGCCA CCCCAACGCC CACTGTCATC ACCCGGGCAG GCCCCCAGGC CCCTGCCAGC
    GGCAGACAGG GGGCAGGCCT CGGGCCCCCA AGTAAGCACA GTGCCCCGGA CCCCCCCAAA
    GCCAAATTCA GCCCTCAGCC TCAGGGTCAG CCGCACGGGA AAGGCGACGG GGCCACCCCT
    CTACCTCCCC GGGAGCCCCC CTCCAAGGCC GGCTCCAGAG GGCCCGCAGT CCCTCAGGGC
    CCACCTGCCC CACACCCTGT CAAGCAGGAG CCGGCGTCCG AGGGCCCCGA GAAGCGCCTG
    GACCTCCTCA GCATCTTCAA GACGTACATC CCGAAGGACC TGGCTTCCCT CTACCAGAGC
    TGGGGCGCCA GCAGCCCTGT GCTCGAGCGC AGAGGGGAGA GACCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.