DDX18 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX18
Entrez Gene ID	519254
Full Name	DEAD-box helicase 18
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005202547.3	XP_005202604.1	ATP-dependent RNA helicase DDX18
XM_005197479.3	XP_005197536.1	ATP-dependent RNA helicase DDX18
XM_005202547.4	XP_005202604.1	ATP-dependent RNA helicase DDX18

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX18	NP_006764.3
Bos taurus (cattle)	DDX18	XP_002685472.1
Danio rerio (zebrafish)	ddx18	NP_001003411.1
Drosophila melanogaster (fruit fly)	pit	NP_732694.2
Eremothecium gossypii	AGOS_AGR333C	NP_986999.2
Arabidopsis thaliana (thale cress)	AT3G18600	NP_188490.1
Pan troglodytes (chimpanzee)	DDX18	XP_515753.2
Canis lupus familiaris (dog)	DDX18	XP_533327.3
Mus musculus (house mouse)	Ddx18	NP_080136.2
Caenorhabditis elegans (roundworm)	B0511.6	NP_492779.1
Saccharomyces cerevisiae (baker's yeast)	HAS1	NP_014017.1
Neurospora crassa	NCU09349	XP_958928.1
Oryza sativa (rice)	Os06g0535100	NP_001057788.2
Xenopus tropicalis (tropical clawed frog)	ddx18	NP_001016776.1
Rattus norvegicus (Norway rat)	Ddx18	NP_001006997.1
Schizosaccharomyces pombe (fission yeast)	has1	NP_594488.1
Gallus gallus (chicken)	DDX18	XP_422125.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008601	XP_314700.4
Kluyveromyces lactis	KLLA0A09669g	XP_451422.1
Magnaporthe oryzae (rice blast fungus)	MGG_06522	XP_003717029.1
Arabidopsis thaliana (thale cress)	AT5G65900	NP_201391.1

Gene Ontology

The following DDX18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02899	XM_005197479.3 Latest version!	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa02899	XM_005202547.4 Latest version!	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa02899	XM_005202547.3	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX18 ( XM_005202547.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202604.1 Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

Price & Availability↑

CloneID	OBa02899
Clone ID Related Accession (Same CDS sequence)	XM_005202547.3 , XM_005197479.3 , XM_005202547.4
Accession Version	XM_005202547.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103842.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202604.1
CDS	92..2374
Translation	MSHLPMKLLRKKIEKRNLKLRQRNLKLQGASAVSLSETQNRDVPEETVGDGKVKKSLKQSVSVGLSEAPNGDIAKETVGSRKVKKSLKQPMSAGLAETQNGDTSEEAGVSGKVKKSRKQSMNASVSEASNGVLPREATENVKAKKSVKESVNVGMSEAQNGDVFKETGENVKVKKASKKSTTLTSGEAAMQSPNSESKKKKKKKKRKVVDDAGPDSKKAKAEDIGEAEDGAQAPEETENRVEKPDDEGEDSEVPSLPLGLTGAFEDTSFDSLTNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLKFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLANGINIVVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCKSVKYHYELLNYIDLPVLAIHGRQKQNKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFEFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNTNDGKVRKRGGGGGFGYQKAKKVEKSKIFKHISKKPSDSRQFSH

Target ORF information:

RefSeq Version	XM_005202547.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202547.3

ORF Insert Sequence:

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX18 ( XM_005197479.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197536.1 Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

Price & Availability↑

CloneID	OBa02899
Clone ID Related Accession (Same CDS sequence)	XM_005202547.3 , XM_005197479.3 , XM_005202547.4
Accession Version	XM_005197479.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644599.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197536.1
CDS	92..2374
Translation	MSHLPMKLLRKKIEKRNLKLRQRNLKLQGASAVSLSETQNRDVPEETVGDGKVKKSLKQSVSVGLSEAPNGDIAKETVGSRKVKKSLKQPMSAGLAETQNGDTSEEAGVSGKVKKSRKQSMNASVSEASNGVLPREATENVKAKKSVKESVNVGMSEAQNGDVFKETGENVKVKKASKKSTTLTSGEAAMQSPNSESKKKKKKKKRKVVDDAGPDSKKAKAEDIGEAEDGAQAPEETENRVEKPDDEGEDSEVPSLPLGLTGAFEDTSFDSLTNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLKFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLANGINIVVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCKSVKYHYELLNYIDLPVLAIHGRQKQNKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFEFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNTNDGKVRKRGGGGGFGYQKAKKVEKSKIFKHISKKPSDSRQFSH

Target ORF information:

RefSeq Version	XM_005197479.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197479.3

ORF Insert Sequence:

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX18 ( XM_005202547.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202604.1 Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

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CloneID	OBa02899
Clone ID Related Accession (Same CDS sequence)	XM_005202547.3 , XM_005197479.3 , XM_005202547.4
Accession Version	XM_005202547.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202547.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202604.1
CDS	107..2389
Translation	MSHLPMKLLRKKIEKRNLKLRQRNLKLQGASAVSLSETQNRDVPEETVGDGKVKKSLKQSVSVGLSEAPNGDIAKETVGSRKVKKSLKQPMSAGLAETQNGDTSEEAGVSGKVKKSRKQSMNASVSEASNGVLPREATENVKAKKSVKESVNVGMSEAQNGDVFKETGENVKVKKASKKSTTLTSGEAAMQSPNSESKKKKKKKKRKVVDDAGPDSKKAKAEDIGEAEDGAQAPEETENRVEKPDDEGEDSEVPSLPLGLTGAFEDTSFDSLTNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLKFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLANGINIVVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCKSVKYHYELLNYIDLPVLAIHGRQKQNKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFEFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNTNDGKVRKRGGGGGFGYQKAKKVEKSKIFKHISKKPSDSRQFSH

Target ORF information:

RefSeq Version	XM_005202547.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 18 (DDX18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202547.4

ORF Insert Sequence:

ATGTCTCATT TACCGATGAA ACTTCTACGC AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGACAACGAA ACCTAAAGCT CCAGGGTGCC TCAGCCGTGA GCCTGTCAGA AACTCAAAAT 
AGAGATGTGC CTGAAGAAAC AGTGGGAGAT GGAAAAGTTA AAAAGTCCTT AAAGCAGTCT 
GTGAGTGTGG GCTTGTCAGA AGCCCCAAAT GGAGATATAG CTAAAGAAAC AGTAGGAAGC 
AGAAAAGTTA AAAAGTCCCT AAAACAACCT ATGAGTGCTG GGTTGGCAGA AACCCAAAAT 
GGAGACACAT CTGAAGAAGC AGGAGTAAGT GGAAAAGTGA AGAAGTCCCG AAAACAATCT 
ATGAATGCAA GCGTGTCGGA AGCCAGCAAT GGAGTCCTGC CTAGGGAAGC CACGGAAAAT 
GTCAAAGCTA AAAAATCTGT TAAAGAGTCT GTAAATGTAG GCATGTCAGA AGCCCAAAAT 
GGAGATGTTT TTAAAGAAAC AGGGGAAAAT GTCAAAGTTA AAAAAGCCTC CAAGAAATCT 
ACCACATTGA CCAGTGGAGA AGCAGCAATG CAGTCTCCCA ATTCAGAATC AAAAAAGAAG 
AAGAAGAAGA AAAAGAGAAA AGTAGTGGAT GATGCTGGGC CTGATTCCAA AAAAGCAAAA 
GCTGAGGACA TAGGAGAGGC TGAAGATGGT GCCCAGGCTC CTGAAGAAAC AGAAAACCGT 
GTGGAGAAGC CAGATGACGA GGGTGAGGAC AGCGAAGTGC CCAGCCTGCC CCTGGGACTG 
ACAGGAGCTT TTGAGGATAC TTCATTTGAT TCTCTGACTA ATCTTGTCAA TGAGAACACT 
CTGAAGGCAA TAAAAGAAAT GGGCTTTACA AACATGACTG AAATTCAACA TAAAAGTATC 
AGACCACTTC TGGAAGGCAG GGATCTTCTA GCAGCTGCAA AAACAGGCAG TGGTAAAACG 
CTGGCATTTC TCATCCCTGC AGTTGAACTC ATTGTTAAGT TAAAGTTCAT GCCCAGGAAT 
GGAACAGGAG TCCTTATTCT CTCACCTACT AGGGAACTGG CCATGCAGAC TTTTGGTGTC 
CTTAAGGAGC TGATGACACA CCATGTTCAC ACATACGGGT TAATAATGGG TGGCAGTAAC 
AGGTCTGCTG AAGCTCAGAA GCTTGCCAAT GGGATCAACA TCGTCGTGGC CACACCAGGC 
CGTCTCCTGG ACCACATGCA GAATACCCCA GGGTTTATGT ATAAAAACCT ACAGTGTCTG 
GTTATTGATG AGGCTGATCG TATCTTGGAT GTTGGGTTTG AAGAGGAATT AAAGCAAATC 
ATTAAACTTC TGCCAACACG CAGACAGACC ATGCTCTTCT CTGCCACACA AACTCGAAAA 
GTTGAAGACC TGGCGAGGAT TTCTCTGAAA AAGGAGCCTT TGTATGTTGG TGTTGATGAT 
GATAAAGCTA ATGCAACTGT AGACGGTCTT GAGCAGGGGT ATGTTGTCTG TCCTTCTGAA 
AAGAGATTCC TCCTGCTCTT CACATTCCTT AAGAAGAACC GGAAGAAGAA ACTAATGGTT 
TTCTTTTCAT CCTGTAAGTC CGTGAAATAC CACTATGAGT TGCTGAACTA CATCGATTTG 
CCTGTCCTGG CCATTCATGG AAGGCAAAAA CAAAATAAGC GTACGACCAC GTTCTTCCAG 
TTTTGCAATG CAGATTCAGG GATCTTGTTG TGTACAGACG TGGCAGCTAG AGGGCTGGAT 
ATTCCTGAAG TGGACTGGAT TGTTCAGTAT GACCCTCCAG ATGACCCCAA GGAGTACATT 
CACCGTGTGG GCAGAACAGC CAGAGGCCTG AATGGAAGAG GGCACGCCTT GCTCATTTTG 
CGCCCTGAAG AATTGGGTTT CCTTCGCTAC CTGAAGCAAT CCAAGGTTCC ATTAAGTGAA 
TTTGAGTTTT CCTGGTCTAA AATTTCTGAC ATTCAGTCTC AGCTTGAAAA ACTGATTGAA 
AAGAACTACT TCCTTCATAA GTCAGCCCAG GAAGCATATA AGTCATACAT TCGAGCCTAT 
GATTCCCATT CCCTGAAACA GATCTTCAAT GTTAATAACT TAAATTTGCC TCAGGTTGCT 
CTGTCATTTG GCTTCAAGGT GCCTCCTTTT GTTGATTTGA ATGTGAATAC CAATGACGGC 
AAAGTTAGAA AGCGAGGCGG TGGCGGTGGA TTTGGCTACC AGAAAGCCAA GAAAGTCGAG 
AAGTCCAAGA TCTTCAAACA CATAAGCAAG AAGCCATCTG ACAGCAGGCA GTTCTCTCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DDX18 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

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