SPIRE1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010818971.2	XP_010817273.1	protein spire homolog 1 isoform X5
XM_005196763.3	XP_005196820.1	protein spire homolog 1 isoform X1
XM_010825576.2	XP_010823878.1	protein spire homolog 1 isoform X5
XM_024984438.1	XP_024840206.1	protein spire homolog 1 isoform X6
XM_005196765.3	XP_005196822.1	protein spire homolog 1 isoform X4
XM_010825575.2	XP_010823877.1	protein spire homolog 1 isoform X3
XM_005224316.4	XP_005224373.1	protein spire homolog 1 isoform X4
XM_010818971.3	XP_010817273.1	protein spire homolog 1 isoform X5
XM_005224314.3	XP_005224371.1	protein spire homolog 1 isoform X1
XM_005224315.3	XP_005224372.1	protein spire homolog 1 isoform X2
XM_010818970.2	XP_010817272.1	protein spire homolog 1 isoform X3
XM_005196764.3	XP_005196821.1	protein spire homolog 1 isoform X2
XM_005224316.3	XP_005224373.1	protein spire homolog 1 isoform X4
XM_005224314.4	XP_005224371.1	protein spire homolog 1 isoform X1
XM_005224315.4	XP_005224372.1	protein spire homolog 1 isoform X2
XM_010818970.3	XP_010817272.1	protein spire homolog 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa49987	XM_010825576.2 Latest version!	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa49987	XM_010818971.3 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa230022	XM_024984438.1 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa11583	XM_005224314.4 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11583	XM_005196763.3 Latest version!	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11584	XM_005224315.4 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11584	XM_005196764.3 Latest version!	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11586	XM_005224316.4 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11586	XM_005196765.3 Latest version!	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa49986	XM_010825575.2 Latest version!	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa49986	XM_010818970.3 Latest version!	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OBa49987	XM_010818971.2	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa11586	XM_005224316.3	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11583	XM_005224314.3	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa11584	XM_005224315.3	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa49986	XM_010818970.2	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

SPIRE1 ( XM_010818971.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817273.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa49987
Clone ID Related Accession (Same CDS sequence)	XM_010818971.2 , XM_010825576.2 , XM_010818971.3
Accession Version	XM_010818971.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817273.1
CDS	124..2094
Translation	MANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010818971.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818971.2

ORF Insert Sequence:

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_010825576.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823878.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa49987
Clone ID Related Accession (Same CDS sequence)	XM_010818971.2 , XM_010825576.2 , XM_010818971.3
Accession Version	XM_010825576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823878.1
CDS	124..2094
Translation	MANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010825576.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825576.2

ORF Insert Sequence:

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_010818971.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817273.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa49987
Clone ID Related Accession (Same CDS sequence)	XM_010818971.2 , XM_010825576.2 , XM_010818971.3
Accession Version	XM_010818971.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817273.1
CDS	220..2190
Translation	MANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010818971.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818971.3

ORF Insert Sequence:

ATGGCCAACA CGGTAGAGGC TGACGGCAGC AATGACGAGG GCTACGAGGC TGCGGATGAG 
GGTCTGGAGG AGGATGATGA CGTAAAGAGG AAGGTCTCAG CCATCCGGTC CTATAAAGAT 
GTCATGAAGT TGTGTGCTGC TCATCTCCCG ACTGAAGCAG AGGCACCAAA TCATTACCAG 
GCAGTATGTC GTGCACTGTT TGCAGAGACG ATGGAGCTGC ATACATTTCT GACCAAAATC 
AAGAGTGCGA AGGAGAATCT GAAGAAGATT CAAGAAATGG AGAAGAGTGA TGAATCTAAC 
ACAGACCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGGT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA GCTCAAGAAG GTCCAGGAGC GGCAGTACAA CCCGCTGCCC 
ATTGAATATC AGCTCACCCC ATACGAGATG CTAATGGATG ACATTCGATC CAGAAGATAC 
ACTTTGCGAA AAGTGATGGT GAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAT 
GAAATCATCC TGGACTTCAT CCGATCAAGG CCTCCTTTAA ATCCAGCCTC AGCCAGAAAA 
CTGAAACCTA CTCCACCACG GCCCCGGAGC CTCCACGAAA GAATCCTGGA AGAGATCAAG 
GCTGAGAGGA AGCTGCGGCC TGTGTCCCCC GAGGAGACCA GACGCAGCAG GCTCGCAACG 
CGGCCTCTTA GCATGTCTTA CAGTTTTGAC TTGTCAGAGG TGACAACCGC TGAATCTCCC 
AAGAATGTTG CAGAATCATC CATGGTGAAT GGAGGTGTGG CGTCACAGCC CAGAGAAGGT 
GGGTCAGGAG CCGCCCAGCA GGTGCCTGTG CCGCGGAAGA AACTCCTCAA AGCCCCGACT 
CTGGCTGAGC TGGACAGCTC CGACTCAGAG GAGGAGACCC TGCCCAGGTC TGCCAGCAGC 
AGCAGTGCAT CTCCCTCCTT CCTGGAAGAC CCCTTCCTGG AGACCGTGTC CACAAGGAAG 
AACCCTCCCA AGTTCTTGCC CATATCCTCC ACACCTCAGC CTGAGCGACG GCAGCCACCC 
CAGAGACGAC ATTCCATTGA GAAGGAAACA CCCACTAATG TGAGACAGTT CCTGCCGGCA 
TCCAGGCAGA GCTCCCGGTC TCTTGTTCCC AGGATGACCA CAGCATGGCC AGCGCCGCCT 
TTCCGACCAC TTTTTTCTAC CCTCCAAACA GCTTCTCTGC TCGGCTCTCA CCCTTTGGAG 
GCAGCCATGC TGGGGGTAGC AGGGGCTGTT TATTATCTGT GTGAGAGAGC TTTTACCAGC 
AGGTGGAGAC ACACACAGGA GGAGTTCTGC TACCCAGTGG AGTGCCTGGC ACTGACCGTG 
GAGGAGGTGA TGCACATTCG TCAGGTCCTG GTCAAAGCAG AGCTGGAAAA GTACCAGCAA 
TACAAAGATG TCTACACGGC TCTGAAAAAA GGAAAGCTCT GCTTTTGTTG CCGAACCAGA 
AGGTTTTCCT TCTTCACCTG GTCTTATACC TGTCAGTTCT GTAAGAGGCC GGTGTGCTCA 
CAGTGTTGCA AAAAGATGCG GCTGCCCTCC AAGCCATACT CCACCCTTCC TATCTTTTCA 
CTGGGACCGT CTGCCTTGCA GAGAGGGGAA AGCTGTATGA GGCCAGAAAA ACCCTCCACT 
GGCCACCATC GGCCACTTCG GAGCATTGCC AGGTTGTCCT CAAAATCCAA GTCTGTGGAC 
AAATCAGATG AAGAACTCCA ATTTCCCAAA GAGCTGGTGG AGGACTGGAG TGCTATGGAG 
GTGTGTGTGG ACTGCAAAAA GTTCATTTCT GAGATCATCA GCTCAAGCCG CCACAGTCTG 
GTGTTGGCCA ACAAGAGAGC TCGATTGAAG AGGAAGACGC AGTCCTTCTA CATGTCCTCA 
GCAGGCCCCT CAGAGTACTG CCCCTCAGAG AGGACCATCA GTGAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_024984438.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840206.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa230022
Clone ID Related Accession (Same CDS sequence)	XM_024984438.1
Accession Version	XM_024984438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGAGGA GACCCTGCCC AGGTCTGCCA GCAGCAGCAG TGCATCTCCC 
TCCTTCCTGG AAGACCCCTT CCTGGAGACC GTGTCCACAA GGAAGAACCC TCCCAAGTTC 
TTGCCCATAT CCTCCACACC TCAGCCTGAG CGACGGCAGC CACCCCAGAG ACGACATTCC 
ATTGAGAAGG AAACACCCAC TAATGTGAGA CAGTTCCTGC CGGCATCCAG GCAGAGCTCC 
CGGTCTCTTG TTCCCAGGAT GACCACAGCA TGGCCAGCGC CGCCTTTCCG ACCACTTTTT 
TCTACCCTCC AAACAGCTTC TCTGCTCGGC TCTCACCCTT TGGAGGCAGC CATGCTGGGG 
GTAGCAGGGG CTGTTTATTA TCTGTGTGAG AGAGCTTTTA CCAGCAGGTG GAGACACACA 
CAGGAGGAGT TCTGCTACCC AGTGGAGTGC CTGGCACTGA CCGTGGAGGA GGTGATGCAC 
ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG GAAAAGTACC AGCAATACAA AGATGTCTAC 
ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC 
ACCTGGTCTT ATACCTGTCA GTTCTGTAAG AGGCCGGTGT GCTCACAGTG TTGCAAAAAG 
ATGCGGCTGC CCTCCAAGCC ATACTCCACC CTTCCTATCT TTTCACTGGG ACCGTCTGCC 
TTGCAGAGAG GGGAAAGCTG TATGAGGCCA GAAAAACCCT CCACTGGCCA CCATCGGCCA 
CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA TCCAAGTCTG TGGACAAATC AGATGAAGAA 
CTCCAATTTC CCAAAGAGCT GGTGGAGGAC TGGAGTGCTA TGGAGGTGTG TGTGGACTGC 
AAAAAGTTCA TTTCTGAGAT CATCAGCTCA AGCCGCCACA GTCTGGTGTT GGCCAACAAG 
AGAGCTCGAT TGAAGAGGAA GACGCAGTCC TTCTACATGT CCTCAGCAGG CCCCTCAGAG 
TACTGCCCCT CAGAGAGGAC CATCAGTGAG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840206.1
CDS	219..2174
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_024984438.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984438.1

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGAGGA GACCCTGCCC AGGTCTGCCA GCAGCAGCAG TGCATCTCCC 
TCCTTCCTGG AAGACCCCTT CCTGGAGACC GTGTCCACAA GGAAGAACCC TCCCAAGTTC 
TTGCCCATAT CCTCCACACC TCAGCCTGAG CGACGGCAGC CACCCCAGAG ACGACATTCC 
ATTGAGAAGG AAACACCCAC TAATGTGAGA CAGTTCCTGC CGGCATCCAG GCAGAGCTCC 
CGGTCTCTTG TTCCCAGGAT GACCACAGCA TGGCCAGCGC CGCCTTTCCG ACCACTTTTT 
TCTACCCTCC AAACAGCTTC TCTGCTCGGC TCTCACCCTT TGGAGGCAGC CATGCTGGGG 
GTAGCAGGGG CTGTTTATTA TCTGTGTGAG AGAGCTTTTA CCAGCAGGTG GAGACACACA 
CAGGAGGAGT TCTGCTACCC AGTGGAGTGC CTGGCACTGA CCGTGGAGGA GGTGATGCAC 
ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG GAAAAGTACC AGCAATACAA AGATGTCTAC 
ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC 
ACCTGGTCTT ATACCTGTCA GTTCTGTAAG AGGCCGGTGT GCTCACAGTG TTGCAAAAAG 
ATGCGGCTGC CCTCCAAGCC ATACTCCACC CTTCCTATCT TTTCACTGGG ACCGTCTGCC 
TTGCAGAGAG GGGAAAGCTG TATGAGGCCA GAAAAACCCT CCACTGGCCA CCATCGGCCA 
CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA TCCAAGTCTG TGGACAAATC AGATGAAGAA 
CTCCAATTTC CCAAAGAGCT GGTGGAGGAC TGGAGTGCTA TGGAGGTGTG TGTGGACTGC 
AAAAAGTTCA TTTCTGAGAT CATCAGCTCA AGCCGCCACA GTCTGGTGTT GGCCAACAAG 
AGAGCTCGAT TGAAGAGGAA GACGCAGTCC TTCTACATGT CCTCAGCAGG CCCCTCAGAG 
TACTGCCCCT CAGAGAGGAC CATCAGTGAG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005196763.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196820.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11583
Clone ID Related Accession (Same CDS sequence)	XM_005196763.3 , XM_005224314.3 , XM_005224314.4
Accession Version	XM_005196763.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196763.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196820.1
CDS	177..2564
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005196763.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196763.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224314.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224371.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11583
Clone ID Related Accession (Same CDS sequence)	XM_005196763.3 , XM_005224314.3 , XM_005224314.4
Accession Version	XM_005224314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224371.1
CDS	177..2564
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224314.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224314.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224314.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224371.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11583
Clone ID Related Accession (Same CDS sequence)	XM_005196763.3 , XM_005224314.3 , XM_005224314.4
Accession Version	XM_005224314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224314.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224371.1
CDS	219..2606
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224314.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224314.4

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGTTCCCAGG ATGACCACAG CATGGCCAGC GCCGCCTTTC 
CGACCACTTT TTTCTACCCT CCAAACAGCT TCTCTGCTCG GCTCTCACCC TTTGGAGGCA 
GCCATGCTGG GGGTAGCAGG GGCTGTTTAT TATCTGTGTG AGAGAGCTTT TACCAGCAGG 
TGGAGACACA CACAGGAGGA GTTCTGCTAC CCAGTGGAGT GCCTGGCACT GACCGTGGAG 
GAGGTGATGC ACATTCGTCA GGTCCTGGTC AAAGCAGAGC TGGAAAAGTA CCAGCAATAC 
AAAGATGTCT ACACGGCTCT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGG 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGCTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAG CCATACTCCA CCCTTCCTAT CTTTTCACTG 
GGACCGTCTG CCTTGCAGAG AGGGGAAAGC TGTATGAGGC CAGAAAAACC CTCCACTGGC 
CACCATCGGC CACTTCGGAG CATTGCCAGG TTGTCCTCAA AATCCAAGTC TGTGGACAAA 
TCAGATGAAG AACTCCAATT TCCCAAAGAG CTGGTGGAGG ACTGGAGTGC TATGGAGGTG 
TGTGTGGACT GCAAAAAGTT CATTTCTGAG ATCATCAGCT CAAGCCGCCA CAGTCTGGTG 
TTGGCCAACA AGAGAGCTCG ATTGAAGAGG AAGACGCAGT CCTTCTACAT GTCCTCAGCA 
GGCCCCTCAG AGTACTGCCC CTCAGAGAGG ACCATCAGTG AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224315.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224372.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa11584
Clone ID Related Accession (Same CDS sequence)	XM_005224315.3 , XM_005196764.3 , XM_005224315.4
Accession Version	XM_005224315.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224372.1
CDS	173..2518
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224315.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224315.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005196764.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196821.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa11584
Clone ID Related Accession (Same CDS sequence)	XM_005224315.3 , XM_005196764.3 , XM_005224315.4
Accession Version	XM_005196764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196821.1
CDS	173..2518
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005196764.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196764.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224315.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224372.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa11584
Clone ID Related Accession (Same CDS sequence)	XM_005224315.3 , XM_005196764.3 , XM_005224315.4
Accession Version	XM_005224315.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224315.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224372.1
CDS	216..2561
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLVPRMTTAWPAPPFRPLFSTLQTASLLGSHPLEAAMLGVAGAVYYLCERAFTSRWRHTQEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224315.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224315.4

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG TTCCCAGGAT GACCACAGCA 
TGGCCAGCGC CGCCTTTCCG ACCACTTTTT TCTACCCTCC AAACAGCTTC TCTGCTCGGC 
TCTCACCCTT TGGAGGCAGC CATGCTGGGG GTAGCAGGGG CTGTTTATTA TCTGTGTGAG 
AGAGCTTTTA CCAGCAGGTG GAGACACACA CAGGAGGAGT TCTGCTACCC AGTGGAGTGC 
CTGGCACTGA CCGTGGAGGA GGTGATGCAC ATTCGTCAGG TCCTGGTCAA AGCAGAGCTG 
GAAAAGTACC AGCAATACAA AGATGTCTAC ACGGCTCTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGGTT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GCTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAGCC ATACTCCACC 
CTTCCTATCT TTTCACTGGG ACCGTCTGCC TTGCAGAGAG GGGAAAGCTG TATGAGGCCA 
GAAAAACCCT CCACTGGCCA CCATCGGCCA CTTCGGAGCA TTGCCAGGTT GTCCTCAAAA 
TCCAAGTCTG TGGACAAATC AGATGAAGAA CTCCAATTTC CCAAAGAGCT GGTGGAGGAC 
TGGAGTGCTA TGGAGGTGTG TGTGGACTGC AAAAAGTTCA TTTCTGAGAT CATCAGCTCA 
AGCCGCCACA GTCTGGTGTT GGCCAACAAG AGAGCTCGAT TGAAGAGGAA GACGCAGTCC 
TTCTACATGT CCTCAGCAGG CCCCTCAGAG TACTGCCCCT CAGAGAGGAC CATCAGTGAG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005196765.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196822.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11586
Clone ID Related Accession (Same CDS sequence)	XM_005196765.3 , XM_005224316.4 , XM_005224316.3
Accession Version	XM_005196765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196822.1
CDS	164..2335
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005196765.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196765.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224316.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224373.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11586
Clone ID Related Accession (Same CDS sequence)	XM_005196765.3 , XM_005224316.4 , XM_005224316.3
Accession Version	XM_005224316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224373.1
CDS	245..2416
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224316.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224316.4

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_005224316.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224373.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11586
Clone ID Related Accession (Same CDS sequence)	XM_005196765.3 , XM_005224316.4 , XM_005224316.3
Accession Version	XM_005224316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224373.1
CDS	164..2335
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_005224316.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224316.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGAGGTGACA 
ACCGCTGAAT CTCCCAAGAA TGTTGCAGAA TCATCCATGG TGAATGGAGG TGTGGCGTCA 
CAGCCCAGAG AAGGTGGGTC AGGAGCCGCC CAGCAGGTGC CTGTGCCGCG GAAGAAACTC 
CTCAAAGCCC CGACTCTGGC TGAGCTGGAC AGCTCCGACT CAGAGGAGGA GACCCTGCCC 
AGGTCTGCCA GCAGCAGCAG TGCATCTCCC TCCTTCCTGG AAGACCCCTT CCTGGAGACC 
GTGTCCACAA GGAAGAACCC TCCCAAGTTC TTGCCCATAT CCTCCACACC TCAGCCTGAG 
CGACGGCAGC CACCCCAGAG ACGACATTCC ATTGAGAAGG AAACACCCAC TAATGTGAGA 
CAGTTCCTGC CGGCATCCAG GCAGAGCTCC CGGTCTCTTG AGGAGTTCTG CTACCCAGTG 
GAGTGCCTGG CACTGACCGT GGAGGAGGTG ATGCACATTC GTCAGGTCCT GGTCAAAGCA 
GAGCTGGAAA AGTACCAGCA ATACAAAGAT GTCTACACGG CTCTGAAAAA AGGAAAGCTC 
TGCTTTTGTT GCCGAACCAG AAGGTTTTCC TTCTTCACCT GGTCTTATAC CTGTCAGTTC 
TGTAAGAGGC CGGTGTGCTC ACAGTGTTGC AAAAAGATGC GGCTGCCCTC CAAGCCATAC 
TCCACCCTTC CTATCTTTTC ACTGGGACCG TCTGCCTTGC AGAGAGGGGA AAGCTGTATG 
AGGCCAGAAA AACCCTCCAC TGGCCACCAT CGGCCACTTC GGAGCATTGC CAGGTTGTCC 
TCAAAATCCA AGTCTGTGGA CAAATCAGAT GAAGAACTCC AATTTCCCAA AGAGCTGGTG 
GAGGACTGGA GTGCTATGGA GGTGTGTGTG GACTGCAAAA AGTTCATTTC TGAGATCATC 
AGCTCAAGCC GCCACAGTCT GGTGTTGGCC AACAAGAGAG CTCGATTGAA GAGGAAGACG 
CAGTCCTTCT ACATGTCCTC AGCAGGCCCC TCAGAGTACT GCCCCTCAGA GAGGACCATC 
AGTGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_010825575.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823877.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49986
Clone ID Related Accession (Same CDS sequence)	XM_010825575.2 , XM_010818970.2 , XM_010818970.3
Accession Version	XM_010825575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823877.1
CDS	171..2384
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010825575.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825575.2

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_010818970.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817272.1 Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49986
Clone ID Related Accession (Same CDS sequence)	XM_010825575.2 , XM_010818970.2 , XM_010818970.3
Accession Version	XM_010818970.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817272.1
CDS	171..2384
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010818970.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spire-type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818970.2

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_010818970.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817272.1 Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

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CloneID	OBa49986
Clone ID Related Accession (Same CDS sequence)	XM_010825575.2 , XM_010818970.2 , XM_010818970.3
Accession Version	XM_010818970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817272.1
CDS	214..2427
Translation	MAQPVGPEGGEGPPDAGAAAGSQDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAADAGRRQPRRRVRSAAQIRLWRDGAVTLAPATGDAGKVEYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDRMANTVEADGSNDEGYEAADEGLEEDDDVKRKVSAIRSYKDVMKLCAAHLPTEAEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSRRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEETRRSRLATRPLSMSYSFDLSEVTTAESPKNVAESSMVNGGVASQPREGGSGAAQQVPVPRKKLLKAPTLAELDSSDSEEETLPRSASSSSASPSFLEDPFLETVSTRKNPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPASRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRPEKPSTGHHRPLRSIARLSSKSKSVDKSDEELQFPKELVEDWSAMEVCVDCKKFISEIISSSRHSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTISEV

Target ORF information:

RefSeq Version	XM_010818970.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818970.3

ORF Insert Sequence:

ATGGCTCAGC CTGTCGGCCC GGAGGGCGGC GAGGGGCCTC CGGACGCCGG GGCGGCCGCG 
GGCTCGCAGG ACGCGCTGAG CCTGGAGGAG ATCCTGCGGC TCTACAATCA GCCCATCAAC 
GAAGAGCAGG CGTGGGCCGT GTGCTACCAG TGCTGCGGCT CCCTGCGCGC CGCGGACGCC 
GGCCGCCGCC AGCCCCGCCG CCGGGTGCGC TCGGCCGCGC AGATCCGCCT GTGGAGGGAC 
GGCGCCGTCA CCTTGGCGCC CGCCACCGGC GACGCGGGTA AAGTGGAATA TTCACATTGT 
ACAGAAACTG AGGTTATAGA ATCTTTGGGA ATTATTATTT ATAAGGCACT GGACTATGGC 
TTGAAGGAGA ATGAAGAAAG GGAGTTAAGC CCACCACTGG AGCAGCTCAT TGATCGCATG 
GCCAACACGG TAGAGGCTGA CGGCAGCAAT GACGAGGGCT ACGAGGCTGC GGATGAGGGT 
CTGGAGGAGG ATGATGACGT AAAGAGGAAG GTCTCAGCCA TCCGGTCCTA TAAAGATGTC 
ATGAAGTTGT GTGCTGCTCA TCTCCCGACT GAAGCAGAGG CACCAAATCA TTACCAGGCA 
GTATGTCGTG CACTGTTTGC AGAGACGATG GAGCTGCATA CATTTCTGAC CAAAATCAAG 
AGTGCGAAGG AGAATCTGAA GAAGATTCAA GAAATGGAGA AGAGTGATGA ATCTAACACA 
GACCTGGAAG AGCTGAAAAA TGCTGACTGG GCTCGGTTCT GGGTACAGGT GATGAGGGAT 
TTGCGGAATG GAGTAAAGCT CAAGAAGGTC CAGGAGCGGC AGTACAACCC GCTGCCCATT 
GAATATCAGC TCACCCCATA CGAGATGCTA ATGGATGACA TTCGATCCAG AAGATACACT 
TTGCGAAAAG TGATGGTGAA TGGTGATATT CCCCCTCGGT TAAAAAAAAG TGCTCATGAA 
ATCATCCTGG ACTTCATCCG ATCAAGGCCT CCTTTAAATC CAGCCTCAGC CAGAAAACTG 
AAACCTACTC CACCACGGCC CCGGAGCCTC CACGAAAGAA TCCTGGAAGA GATCAAGGCT 
GAGAGGAAGC TGCGGCCTGT GTCCCCCGAG GAGACCAGAC GCAGCAGGCT CGCAACGCGG 
CCTCTTAGCA TGTCTTACAG TTTTGACTTG TCAGAGGTGA CAACCGCTGA ATCTCCCAAG 
AATGTTGCAG AATCATCCAT GGTGAATGGA GGTGTGGCGT CACAGCCCAG AGAAGGTGGG 
TCAGGAGCCG CCCAGCAGGT GCCTGTGCCG CGGAAGAAAC TCCTCAAAGC CCCGACTCTG 
GCTGAGCTGG ACAGCTCCGA CTCAGAGGAG GAGACCCTGC CCAGGTCTGC CAGCAGCAGC 
AGTGCATCTC CCTCCTTCCT GGAAGACCCC TTCCTGGAGA CCGTGTCCAC AAGGAAGAAC 
CCTCCCAAGT TCTTGCCCAT ATCCTCCACA CCTCAGCCTG AGCGACGGCA GCCACCCCAG 
AGACGACATT CCATTGAGAA GGAAACACCC ACTAATGTGA GACAGTTCCT GCCGGCATCC 
AGGCAGAGCT CCCGGTCTCT TGAGGAGTTC TGCTACCCAG TGGAGTGCCT GGCACTGACC 
GTGGAGGAGG TGATGCACAT TCGTCAGGTC CTGGTCAAAG CAGAGCTGGA AAAGTACCAG 
CAATACAAAG ATGTCTACAC GGCTCTGAAA AAAGGAAAGC TCTGCTTTTG TTGCCGAACC 
AGAAGGTTTT CCTTCTTCAC CTGGTCTTAT ACCTGTCAGT TCTGTAAGAG GCCGGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCC TCCAAGCCAT ACTCCACCCT TCCTATCTTT 
TCACTGGGAC CGTCTGCCTT GCAGAGAGGG GAAAGCTGTA TGAGGCCAGA AAAACCCTCC 
ACTGGCCACC ATCGGCCACT TCGGAGCATT GCCAGGTTGT CCTCAAAATC CAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAATTTCCC AAAGAGCTGG TGGAGGACTG GAGTGCTATG 
GAGGTGTGTG TGGACTGCAA AAAGTTCATT TCTGAGATCA TCAGCTCAAG CCGCCACAGT 
CTGGTGTTGG CCAACAAGAG AGCTCGATTG AAGAGGAAGA CGCAGTCCTT CTACATGTCC 
TCAGCAGGCC CCTCAGAGTA CTGCCCCTCA GAGAGGACCA TCAGTGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPIRE1
Entrez Gene ID	519030
Full Name	spire type actin nucleation factor 1
General protein information	Preferred Names spire type actin nucleation factor 1 Names protein spire homolog 1 spire homolog 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	SPIRE1	XP_547684.4
Gallus gallus (chicken)	SPIRE1	XP_004939777.1
Mus musculus (house mouse)	Spire1	NP_919336.1
Xenopus tropicalis (tropical clawed frog)	spire1	XP_002936125.2
Pan troglodytes (chimpanzee)	SPIRE1	XP_003315842.1
Rattus norvegicus (Norway rat)	Spire1	NP_001100851.1
Danio rerio (zebrafish)	spire1a	NP_001038312.2
Homo sapiens (human)	SPIRE1	NP_001122098.1
Bos taurus (cattle)	SPIRE1	XP_005224371.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPIRE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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