ATP2A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP2A1
Entrez Gene ID	518117
Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
Synonyms	SERCA1
General protein information	Preferred Names ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 Names sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ATPase, Ca++ transporting, fast twitch 1 SR Ca(2+)-ATPase 1 calcium pump 1 calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984881.1	XP_024840649.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform X1
NM_001075767.1	NP_001069235.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP2A1	NP_775293.1
Pan troglodytes (chimpanzee)	ATP2A1	XP_001142958.3
Magnaporthe oryzae (rice blast fungus)	MGG_04550	XP_003710933.1
Arabidopsis thaliana (thale cress)	ECA3	NP_563860.1
Xenopus tropicalis (tropical clawed frog)	atp2a1	NP_001027498.1
Rattus norvegicus (Norway rat)	Atp2a1	NP_478120.1
Neurospora crassa	NCU03305	XP_964482.2
Mus musculus (house mouse)	Atp2a1	NP_031530.2
Danio rerio (zebrafish)	atp2a1	NP_001007030.1
Caenorhabditis elegans (roundworm)	sca-1	NP_499386.1
Oryza sativa (rice)	Os03g0730800	NP_001051163.2
Danio rerio (zebrafish)	atp2a1l	NP_001071001.1
Anopheles gambiae (African malaria mosquito)	ATC1_ANOGA	XP_001688815.1
Canis lupus familiaris (dog)	ATP2A1	XP_005621437.1
Bos taurus (cattle)	ATP2A1	NP_001069235.1
Drosophila melanogaster (fruit fly)	Ca-P60A	NP_726387.1

Gene Ontology
Bibliography

Related articles in PubMed

Fast-twitch skeletal muscle fiber adaptation to SERCA1 deficiency in a Dutch Improved Red and White calf pseudomyotonia case.
Dorotea T, Gr?nberg W, Murgiano L, Plattet P, Dr?gem?ller C, Mascarello F, Sacchetto R
Neuromuscular disorders : NMD25(11)888-97(2015 Nov)

Inhibition of ubiquitin proteasome system rescues the defective sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1) protein causing Chianina cattle pseudomyotonia.
Bianchini E, Testoni S, Gentile A, Cal? T, Ottolini D, Villa A, Brini M, Betto R, Mascarello F, Nissen P, Sandon? D, Sacchetto R
The Journal of biological chemistry289(48)33073-82(2014 Nov)

Crystal structure of sarcoplasmic reticulum Ca2+-ATPase (SERCA) from bovine muscle.
Sacchetto R, Bertipaglia I, Giannetti S, Cendron L, Mascarello F, Damiani E, Carafoli E, Zanotti G
Journal of structural biology178(1)38-44(2012 Apr)

A defective SERCA1 protein is responsible for congenital pseudomyotonia in Chianina cattle.
Sacchetto R, Testoni S, Gentile A, Damiani E, Rossi M, Liguori R, Dr?gem?ller C, Mascarello F
The American journal of pathology174(2)565-73(2009 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that a cattle congenital pseudomyotonia is caused by a SERCA1 deficiency, resulting from a defect in the ATP2A1 gene
Title: Fast-twitch skeletal muscle fiber adaptation to SERCA1 deficiency in a Dutch Improved Red and White calf pseudomyotonia case.

Rescue of mutated SERCA1 to sarcoplasmic reticulum membrane can re-establish resting cytosolic Ca(2+) concentration and prevent the appearance of pathological signs of cattle pseudomyotonia
Title: Inhibition of ubiquitin proteasome system rescues the defective sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1) protein causing Chianina cattle pseudomyotonia.

crystal structure of SERCA, crystallized in the E1 conformation and determined at 2.9A resolution
Title: Crystal structure of sarcoplasmic reticulum Ca2+-ATPase (SERCA) from bovine muscle.

In this study, we provide biochemical evidence for a selective deficiency in SERCA1 protein levels in sarcoplasmic reticulum membranes from Chianina cattle with congenital pseudomyotonia
Title: A defective SERCA1 protein is responsible for congenital pseudomyotonia in Chianina cattle.

study reports the identification of a missense mutation in ATP2A1 in congential pseudomytonia of Chianina cattle; mutation analysis of ATP2A1 exons revealed a perfectly associated missense mutation in exon 6 (c.491G>A) leading to p.Arg164His substitution
Title: Identification of a missense mutation in the bovine ATP2A1 gene in congenital pseudomyotonia of Chianina cattle: an animal model of human Brody disease.

The following ATP2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42043	NM_001075767.1 Latest version!	Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa230283	XM_024984881.1 Latest version!	Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP2A1 ( NM_001075767.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069235.1 Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), mRNA.

Price & Availability↑

CloneID	OBa42043
Clone ID Related Accession (Same CDS sequence)	NM_001075767.1
Accession Version	NM_001075767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC119938.1. On or before Oct 4, 2006 this sequence version replaced XM_596301.2, XM_878941.1, XM_878969.1, XM_878994.1, XM_879022.1, XM_879057.1, XM_879088.1, XM_879115.1, XM_866816.1. ##Evidence-Data-START## Transcript exon combination :: BC119938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145459, SAMN03145460 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCTG CGCACTCCAA GACCACAGAA GAATGTTTGG CCTATTTTGG GGTGAGCGAA 
ACTACAGGCC TCACCCCGGA CCAAGTTAAG CGGCATCTGG AGAAATATGG CCATAATGAG 
CTCCCTGCTG AGGAGGGGAA GTCCCTGTGG GAGCTGGTGT TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TCCTTCTCCT GGCCGCCTGC ATTTCCTTTG TGCTGGCCTG GTTTGAGGAA 
GGCGAAGAGA CTGTCACCGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AATGCAGAGA ATGCCATCGA GGCTCTGAAG 
GAATATGAAC CCGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT ACAAAGGATC 
AAAGCTCGGG ATATTGTTCC TGGAGACATC GTGGAGGTGG CTGTGGGGGA CAAGGTCCCG 
GCAGATATCC GCATCCTCAC CATCAAGTCT ACCACCCTCC GGGTCGACCA GTCCATCCTG 
ACAGGCGAGT CTGTCTCTGT CATCAAGCAC ACAGAACCTG TTCCCGACCC CCGAGCTGTC 
AACCAGGATA AGAAGAACAT GCTTTTCTCG GGCACCAACA TCGCAGCCGG CAAGGCCATC 
GGCATTGTGG CCACCACCGG TGTGGGCACC GAGATTGGGA AGATCCGTGA CCAAATGGCC 
GCCACAGAGC AGGACAAGAC CCCTCTGCAG CAAAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGCGTGGCC GTCTGGCTCA TCAACATTGG CCACTTCAAC 
GACCCCGTGC ATGGGGGCTC CTGGATCCGT GGTGCCATCT ACTACTTTAA GATCGCCGTG 
GCCCTGGCTG TGGCTGCCAT CCCCGAGGGC CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
TTGGGCACCC GCCGGATGGC AAAGAAGAAC GCGATCGTGA GGAGCCTGCC CTCCGTGGAG 
ACTCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACGG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT CATCATCGAC AGGATAGATG GGGACCTCTG CCTGCTGAAC 
GAGTTCTCTG TCACTGGCTC CACTTACGCT CCTGAAGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGCT CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATTTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAATGAGACC AAAGGTATTT ATGAGAAGGT GGGAGAGGCC 
ACCGAGACAG CCCTCACCAC CCTGGTGGAG AAGATGAATG TATTCAACAC TGAAGTGAGA 
AACCTCTCGA AGGTAGAGAG GGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGGGAC CGAAAGTCCA TGTCTGTCTA TTGCTCTCCA 
GCAAAATCCC GGGCTGCTGT GGGCAATAAG ATGTTTGTCA AGGGTGCCCC TGAGGGGGTC 
ATCGATCGCT GTAACTACGT GCGAGTGGGC ACCACCCGGG TGCCCATGAC AGGGCCGGTG 
AAGGAGAAGA TTCTGTCGGT GATCAAAGAG TGGGGTACTG GCCGGGACAC CCTGCGCTGC 
CTGGCGCTGG CCACTCGGGA CACCCCCCCC AAGCGAGAGG AGATGGTCCT GGATGACTCT 
ACCAAGTTCA TGGAGTACGA GACGGACTTG ACATTCGTTG GCGTCGTGGG CATGCTGGAC 
CCGCCCCGCA AGGAGGTCAT GGGCTCCATC CAACTATGCC GAGACGCTGG AATCCGAGTA 
ATCATGATCA CCGGAGACAA CAAGGGCACG GCCATTGCCA TCTGCCGACG GATTGGCATC 
TTCGGGGAGA ACGAGGATGT GGCTGACCGG GCCTACACTG GCCGGGAGTT CGATGACCTG 
CCCCTGGCCG AGCAGCGGGA GGCCTGCCGA CGCGCCTGCT GCTTCGCCCG CGTGGAGCCC 
ACCCACAAGT CGAAGATCGT GGAGTATCTG CAGTCCTTCG ATGAGATCAC GGCGATGACA 
GGTGATGGCG TCAATGACGC CCCTGCGCTG AAGAAGGCGG AAATCGGCAT TGCCATGGGA 
TCCGGCACTG CCGTGGCCAA GACAGCCTCT GAGATGGTGC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCCGTGGA GGAGGGCCGG GCCATCTACA ACAACATGAA GCAGTTTATC 
CGCTACCTCA TCTCCTCCAA CGTGGGCGAG GTGGTCTGCA TCTTCCTGAC AGCTGCCCTG 
GGGCTGCCCG AGGCCCTGAT CCCTGTGCAG CTGCTGTGGG TGAACCTGGT GACCGATGGG 
CTCCCAGCCA CAGCCCTGGG CTTCAACCCC CCAGACCTGG ACATCATGGA CCGGCCCCCC 
CGGACCCCCA AGGAGCCCCT CATCAGTGGC TGGCTCTTCT TCCGCTACAT GGCAATTGGG 
GGCTATGTGG GTGCAGCCAC CGTGGGAGCA GCGGCCTGGT GGTTCCTGTA TGCTGAGGAC 
GGGCCTCACG TCACCTACAG CCAGCTGACT CACTTCATGA AGTGCTCGGA GCACAGCCCA 
GACTTCGAGG GTGTGGACTG TGAGGTCTTC GAGGCCCCGC AGCCCATGAC CATGGCCTTG 
TCTGTGCTGG TCACCATCGA GATGTGCAAT GCTCTCAACA GCCTGTCTGA GAATCAGTCC 
CTCGTGCGGA TGCCACCCTG GGTGAACATC TGGCTGGTGG GCTCCATCGG CCTCTCCATG 
TCCCTCCACT TCCTCATCCT CTACGTGGAC CCTCTGCCGA TGATCTTCAA GCTCCAAGCC 
CTGGACCTGT ACCATTGGCT CATGGTCCTC AAGATCTCAT TACCAGTCAT TGGGCTCGAC 
GAAATCCTCA AGTTCGTTGC TCGAAACTAC CTGGAGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069235.1
CDS	171..3152
Translation	MEAAHSKTTEECLAYFGVSETTGLTPDQVKRHLEKYGHNELPAEEGKSLWELVLEQFEDLLVRILLLAACISFVLAWFEEGEETVTAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILTIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKAIGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDRIDGDLCLLNEFSVTGSTYAPEGEVLKNDKPVRSGQYDGLVELATICALCNDSSLDFNETKGIYEKVGEATETALTTLVEKMNVFNTEVRNLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPMTGPVKEKILSVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSTKFMEYETDLTFVGVVGMLDPPRKEVMGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEDVADRAYTGREFDDLPLAEQREACRRACCFARVEPTHKSKIVEYLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRTPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVTYSQLTHFMKCSEHSPDFEGVDCEVFEAPQPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWVNIWLVGSIGLSMSLHFLILYVDPLPMIFKLQALDLYHWLMVLKISLPVIGLDEILKFVARNYLEG

Target ORF information:

RefSeq Version	NM_001075767.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075767.1

ORF Insert Sequence:

ATGGAGGCTG CGCACTCCAA GACCACAGAA GAATGTTTGG CCTATTTTGG GGTGAGCGAA 
ACTACAGGCC TCACCCCGGA CCAAGTTAAG CGGCATCTGG AGAAATATGG CCATAATGAG 
CTCCCTGCTG AGGAGGGGAA GTCCCTGTGG GAGCTGGTGT TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TCCTTCTCCT GGCCGCCTGC ATTTCCTTTG TGCTGGCCTG GTTTGAGGAA 
GGCGAAGAGA CTGTCACCGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AATGCAGAGA ATGCCATCGA GGCTCTGAAG 
GAATATGAAC CCGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT ACAAAGGATC 
AAAGCTCGGG ATATTGTTCC TGGAGACATC GTGGAGGTGG CTGTGGGGGA CAAGGTCCCG 
GCAGATATCC GCATCCTCAC CATCAAGTCT ACCACCCTCC GGGTCGACCA GTCCATCCTG 
ACAGGCGAGT CTGTCTCTGT CATCAAGCAC ACAGAACCTG TTCCCGACCC CCGAGCTGTC 
AACCAGGATA AGAAGAACAT GCTTTTCTCG GGCACCAACA TCGCAGCCGG CAAGGCCATC 
GGCATTGTGG CCACCACCGG TGTGGGCACC GAGATTGGGA AGATCCGTGA CCAAATGGCC 
GCCACAGAGC AGGACAAGAC CCCTCTGCAG CAAAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGCGTGGCC GTCTGGCTCA TCAACATTGG CCACTTCAAC 
GACCCCGTGC ATGGGGGCTC CTGGATCCGT GGTGCCATCT ACTACTTTAA GATCGCCGTG 
GCCCTGGCTG TGGCTGCCAT CCCCGAGGGC CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
TTGGGCACCC GCCGGATGGC AAAGAAGAAC GCGATCGTGA GGAGCCTGCC CTCCGTGGAG 
ACTCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACGG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT CATCATCGAC AGGATAGATG GGGACCTCTG CCTGCTGAAC 
GAGTTCTCTG TCACTGGCTC CACTTACGCT CCTGAAGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGCT CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATTTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAATGAGACC AAAGGTATTT ATGAGAAGGT GGGAGAGGCC 
ACCGAGACAG CCCTCACCAC CCTGGTGGAG AAGATGAATG TATTCAACAC TGAAGTGAGA 
AACCTCTCGA AGGTAGAGAG GGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGGGAC CGAAAGTCCA TGTCTGTCTA TTGCTCTCCA 
GCAAAATCCC GGGCTGCTGT GGGCAATAAG ATGTTTGTCA AGGGTGCCCC TGAGGGGGTC 
ATCGATCGCT GTAACTACGT GCGAGTGGGC ACCACCCGGG TGCCCATGAC AGGGCCGGTG 
AAGGAGAAGA TTCTGTCGGT GATCAAAGAG TGGGGTACTG GCCGGGACAC CCTGCGCTGC 
CTGGCGCTGG CCACTCGGGA CACCCCCCCC AAGCGAGAGG AGATGGTCCT GGATGACTCT 
ACCAAGTTCA TGGAGTACGA GACGGACTTG ACATTCGTTG GCGTCGTGGG CATGCTGGAC 
CCGCCCCGCA AGGAGGTCAT GGGCTCCATC CAACTATGCC GAGACGCTGG AATCCGAGTA 
ATCATGATCA CCGGAGACAA CAAGGGCACG GCCATTGCCA TCTGCCGACG GATTGGCATC 
TTCGGGGAGA ACGAGGATGT GGCTGACCGG GCCTACACTG GCCGGGAGTT CGATGACCTG 
CCCCTGGCCG AGCAGCGGGA GGCCTGCCGA CGCGCCTGCT GCTTCGCCCG CGTGGAGCCC 
ACCCACAAGT CGAAGATCGT GGAGTATCTG CAGTCCTTCG ATGAGATCAC GGCGATGACA 
GGTGATGGCG TCAATGACGC CCCTGCGCTG AAGAAGGCGG AAATCGGCAT TGCCATGGGA 
TCCGGCACTG CCGTGGCCAA GACAGCCTCT GAGATGGTGC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCCGTGGA GGAGGGCCGG GCCATCTACA ACAACATGAA GCAGTTTATC 
CGCTACCTCA TCTCCTCCAA CGTGGGCGAG GTGGTCTGCA TCTTCCTGAC AGCTGCCCTG 
GGGCTGCCCG AGGCCCTGAT CCCTGTGCAG CTGCTGTGGG TGAACCTGGT GACCGATGGG 
CTCCCAGCCA CAGCCCTGGG CTTCAACCCC CCAGACCTGG ACATCATGGA CCGGCCCCCC 
CGGACCCCCA AGGAGCCCCT CATCAGTGGC TGGCTCTTCT TCCGCTACAT GGCAATTGGG 
GGCTATGTGG GTGCAGCCAC CGTGGGAGCA GCGGCCTGGT GGTTCCTGTA TGCTGAGGAC 
GGGCCTCACG TCACCTACAG CCAGCTGACT CACTTCATGA AGTGCTCGGA GCACAGCCCA 
GACTTCGAGG GTGTGGACTG TGAGGTCTTC GAGGCCCCGC AGCCCATGAC CATGGCCTTG 
TCTGTGCTGG TCACCATCGA GATGTGCAAT GCTCTCAACA GCCTGTCTGA GAATCAGTCC 
CTCGTGCGGA TGCCACCCTG GGTGAACATC TGGCTGGTGG GCTCCATCGG CCTCTCCATG 
TCCCTCCACT TCCTCATCCT CTACGTGGAC CCTCTGCCGA TGATCTTCAA GCTCCAAGCC 
CTGGACCTGT ACCATTGGCT CATGGTCCTC AAGATCTCAT TACCAGTCAT TGGGCTCGAC 
GAAATCCTCA AGTTCGTTGC TCGAAACTAC CTGGAGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( XM_024984881.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840649.1 Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230283
Clone ID Related Accession (Same CDS sequence)	XM_024984881.1
Accession Version	XM_024984881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG CGCACTCCAA GACCACAGAA GAATGTTTGG CCTATTTTGG GGTGAGCGAA 
ACTACAGGCC TCACCCCGGA CCAAGTTAAG CGGCATCTGG AGAAATATGG CCATAATGAG 
CTCCCTGCTG AGGAGGGGAA GTCCCTGTGG GAGCTGGTGT TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TCCTTCTCCT GGCCGCCTGC ATTTCCTTTG TGCTGGCCTG GTTTGAGGAA 
GGCGAAGAGA CTGTCACCGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AATGCAGAGA ATGCCATCGA GGCTCTGAAG 
GAATATGAAC CCGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT ACAAAGGATC 
AAAGCTCGGG ATATTGTTCC TGGAGACATC GTGGAGGTGG CTGTGGGGGA CAAGGTCCCG 
GCAGATATCC GCATCCTCAC CATCAAGTCT ACCACCCTCC GGGTCGACCA GTCCATCCTG 
ACAGGCGAGT CTGTCTCTGT CATCAAGCAC ACAGAACCTG TTCCCGACCC CCGAGCTGTC 
AACCAGGATA AGAAGAACAT GCTTTTCTCG GGCACCAACA TCGCAGCCGG CAAGGCCATC 
GGCATTGTGG CCACCACCGG TGTGGGCACC GAGATTGGGA AGATCCGTGA CCAAATGGCC 
GCCACAGAGC AGGACAAGAC CCCTCTGCAG CAAAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGCGTGGCC GTCTGGCTCA TCAACATTGG CCACTTCAAC 
GACCCCGTGC ATGGGGGCTC CTGGATCCGT GGTGCCATCT ACTACTTTAA GATCGCCGTG 
GCCCTGGCTG TGGCTGCCAT CCCCGAGGGC CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
TTGGGCACCC GCCGGATGGC AAAGAAGAAC GCGATCGTGA GGAGCCTGCC CTCCGTGGAG 
ACTCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACGG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT CATCATCGAC AGGATAGATG GGGACCTCTG CCTGCTGAAC 
GAGTTCTCTG TCACTGGCTC CACTTACGCT CCTGAAGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGCT CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATTTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAATGAGACC AAAGGTATTT ATGAGAAGGT GGGAGAGGCC 
ACCGAGACAG CCCTCACCAC CCTGGTGGAG AAGATGAATG TATTCAACAC TGAAGTGAGA 
AACCTCTCGA AGGTAGAGAG GGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGGGAC CGAAAGTCCA TGTCTGTCTA TTGCTCTCCA 
GCAAAATCCC GGGCTGCTGT GGGCAATAAG ATGTTTGTCA AGGGTGCCCC TGAGGGGGTC 
ATCGATCGCT GTAACTACGT GCGAGTGGGC ACCACCCGGG TGCCCATGAC AGGGCCGGTG 
AAGGAGAAGA TTCTGTCGGT GATCAAAGAG TGGGGTACTG GCCGGGACAC CCTGCGCTGC 
CTGGCGCTGG CCACTCGGGA CACCCCCCCC AAGCGAGAGG AGATGGTCCT GGATGACTCT 
ACCAAGTTCA TGGAGTACGA GACGGACTTG ACATTCGTTG GCGTCGTGGG CATGCTGGAC 
CCGCCCCGCA AGGAGGTCAT GGGCTCCATC CAACTATGCC GAGACGCTGG AATCCGAGTA 
ATCATGATCA CCGGAGACAA CAAGGGCACG GCCATTGCCA TCTGCCGACG GATTGGCATC 
TTCGGGGAGA ACGAGGATGT GGCTGACCGG GCCTACACTG GCCGGGAGTT CGATGACCTG 
CCCCTGGCCG AGCAGCGGGA GGCCTGCCGA CGCGCCTGCT GCTTCGCCCG CGTGGAGCCC 
ACCCACAAGT CGAAGATCGT GGAGTATCTG CAGTCCTTCG ATGAGATCAC GGCGATGACA 
GGTGATGGCG TCAATGACGC CCCTGCGCTG AAGAAGGCGG AAATCGGCAT TGCCATGGGA 
TCCGGCACTG CCGTGGCCAA GACAGCCTCT GAGATGGTGC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCCGTGGA GGAGGGCCGG GCCATCTACA ACAACATGAA GCAGTTTATC 
CGCTACCTCA TCTCCTCCAA CGTGGGCGAG GTGGTCTGCA TCTTCCTGAC AGCTGCCCTG 
GGGCTGCCCG AGGCCCTGAT CCCTGTGCAG CTGCTGTGGG TGAACCTGGT GACCGATGGG 
CTCCCAGCCA CAGCCCTGGG CTTCAACCCC CCAGACCTGG ACATCATGGA CCGGCCCCCC 
CGGACCCCCA AGGAGCCCCT CATCAGTGGC TGGCTCTTCT TCCGCTACAT GGCAATTGGG 
GGCTATGTGG GTGCAGCCAC CGTGGGAGCA GCGGCCTGGT GGTTCCTGTA TGCTGAGGAC 
GGGCCTCACG TCACCTACAG CCAGCTGACT CACTTCATGA AGTGCTCGGA GCACAGCCCA 
GACTTCGAGG GTGTGGACTG TGAGGTCTTC GAGGCCCCGC AGCCCATGAC CATGGCCTTG 
TCTGTGCTGG TCACCATCGA GATGTGCAAT GCTCTCAACA GCCTGTCTGA GAATCAGTCC 
CTCGTGCGGA TGCCACCCTG GGTGAACATC TGGCTGGTGG GCTCCATCGG CCTCTCCATG 
TCCCTCCACT TCCTCATCCT CTACGTGGAC CCTCTGCCGA TGATCTTCAA GCTCCAAGCC 
CTGGACCTGT ACCATTGGCT CATGGTCCTC AAGATCTCAT TACCAGTCAT TGGGCTCGAC 
GAAATCCTCA AGTTCGTTGC TCGAAACTAC CTGGAGGATC CAGAAGATGA AAGAAGGAAG 
CAACCAGGCC TTTTGCCCTC CCTCCCCACC CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840649.1
CDS	201..3236
Translation	MEAAHSKTTEECLAYFGVSETTGLTPDQVKRHLEKYGHNELPAEEGKSLWELVLEQFEDLLVRILLLAACISFVLAWFEEGEETVTAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILTIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKAIGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDRIDGDLCLLNEFSVTGSTYAPEGEVLKNDKPVRSGQYDGLVELATICALCNDSSLDFNETKGIYEKVGEATETALTTLVEKMNVFNTEVRNLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPMTGPVKEKILSVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSTKFMEYETDLTFVGVVGMLDPPRKEVMGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEDVADRAYTGREFDDLPLAEQREACRRACCFARVEPTHKSKIVEYLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRTPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVTYSQLTHFMKCSEHSPDFEGVDCEVFEAPQPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWVNIWLVGSIGLSMSLHFLILYVDPLPMIFKLQALDLYHWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRKQPGLLPSLPTP

Target ORF information:

RefSeq Version	XM_024984881.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984881.1

ORF Insert Sequence:

ATGGAGGCTG CGCACTCCAA GACCACAGAA GAATGTTTGG CCTATTTTGG GGTGAGCGAA 
ACTACAGGCC TCACCCCGGA CCAAGTTAAG CGGCATCTGG AGAAATATGG CCATAATGAG 
CTCCCTGCTG AGGAGGGGAA GTCCCTGTGG GAGCTGGTGT TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TCCTTCTCCT GGCCGCCTGC ATTTCCTTTG TGCTGGCCTG GTTTGAGGAA 
GGCGAAGAGA CTGTCACCGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AATGCAGAGA ATGCCATCGA GGCTCTGAAG 
GAATATGAAC CCGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT ACAAAGGATC 
AAAGCTCGGG ATATTGTTCC TGGAGACATC GTGGAGGTGG CTGTGGGGGA CAAGGTCCCG 
GCAGATATCC GCATCCTCAC CATCAAGTCT ACCACCCTCC GGGTCGACCA GTCCATCCTG 
ACAGGCGAGT CTGTCTCTGT CATCAAGCAC ACAGAACCTG TTCCCGACCC CCGAGCTGTC 
AACCAGGATA AGAAGAACAT GCTTTTCTCG GGCACCAACA TCGCAGCCGG CAAGGCCATC 
GGCATTGTGG CCACCACCGG TGTGGGCACC GAGATTGGGA AGATCCGTGA CCAAATGGCC 
GCCACAGAGC AGGACAAGAC CCCTCTGCAG CAAAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGCGTGGCC GTCTGGCTCA TCAACATTGG CCACTTCAAC 
GACCCCGTGC ATGGGGGCTC CTGGATCCGT GGTGCCATCT ACTACTTTAA GATCGCCGTG 
GCCCTGGCTG TGGCTGCCAT CCCCGAGGGC CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
TTGGGCACCC GCCGGATGGC AAAGAAGAAC GCGATCGTGA GGAGCCTGCC CTCCGTGGAG 
ACTCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACGG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT CATCATCGAC AGGATAGATG GGGACCTCTG CCTGCTGAAC 
GAGTTCTCTG TCACTGGCTC CACTTACGCT CCTGAAGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGCT CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATTTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAATGAGACC AAAGGTATTT ATGAGAAGGT GGGAGAGGCC 
ACCGAGACAG CCCTCACCAC CCTGGTGGAG AAGATGAATG TATTCAACAC TGAAGTGAGA 
AACCTCTCGA AGGTAGAGAG GGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGGGAC CGAAAGTCCA TGTCTGTCTA TTGCTCTCCA 
GCAAAATCCC GGGCTGCTGT GGGCAATAAG ATGTTTGTCA AGGGTGCCCC TGAGGGGGTC 
ATCGATCGCT GTAACTACGT GCGAGTGGGC ACCACCCGGG TGCCCATGAC AGGGCCGGTG 
AAGGAGAAGA TTCTGTCGGT GATCAAAGAG TGGGGTACTG GCCGGGACAC CCTGCGCTGC 
CTGGCGCTGG CCACTCGGGA CACCCCCCCC AAGCGAGAGG AGATGGTCCT GGATGACTCT 
ACCAAGTTCA TGGAGTACGA GACGGACTTG ACATTCGTTG GCGTCGTGGG CATGCTGGAC 
CCGCCCCGCA AGGAGGTCAT GGGCTCCATC CAACTATGCC GAGACGCTGG AATCCGAGTA 
ATCATGATCA CCGGAGACAA CAAGGGCACG GCCATTGCCA TCTGCCGACG GATTGGCATC 
TTCGGGGAGA ACGAGGATGT GGCTGACCGG GCCTACACTG GCCGGGAGTT CGATGACCTG 
CCCCTGGCCG AGCAGCGGGA GGCCTGCCGA CGCGCCTGCT GCTTCGCCCG CGTGGAGCCC 
ACCCACAAGT CGAAGATCGT GGAGTATCTG CAGTCCTTCG ATGAGATCAC GGCGATGACA 
GGTGATGGCG TCAATGACGC CCCTGCGCTG AAGAAGGCGG AAATCGGCAT TGCCATGGGA 
TCCGGCACTG CCGTGGCCAA GACAGCCTCT GAGATGGTGC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCCGTGGA GGAGGGCCGG GCCATCTACA ACAACATGAA GCAGTTTATC 
CGCTACCTCA TCTCCTCCAA CGTGGGCGAG GTGGTCTGCA TCTTCCTGAC AGCTGCCCTG 
GGGCTGCCCG AGGCCCTGAT CCCTGTGCAG CTGCTGTGGG TGAACCTGGT GACCGATGGG 
CTCCCAGCCA CAGCCCTGGG CTTCAACCCC CCAGACCTGG ACATCATGGA CCGGCCCCCC 
CGGACCCCCA AGGAGCCCCT CATCAGTGGC TGGCTCTTCT TCCGCTACAT GGCAATTGGG 
GGCTATGTGG GTGCAGCCAC CGTGGGAGCA GCGGCCTGGT GGTTCCTGTA TGCTGAGGAC 
GGGCCTCACG TCACCTACAG CCAGCTGACT CACTTCATGA AGTGCTCGGA GCACAGCCCA 
GACTTCGAGG GTGTGGACTG TGAGGTCTTC GAGGCCCCGC AGCCCATGAC CATGGCCTTG 
TCTGTGCTGG TCACCATCGA GATGTGCAAT GCTCTCAACA GCCTGTCTGA GAATCAGTCC 
CTCGTGCGGA TGCCACCCTG GGTGAACATC TGGCTGGTGG GCTCCATCGG CCTCTCCATG 
TCCCTCCACT TCCTCATCCT CTACGTGGAC CCTCTGCCGA TGATCTTCAA GCTCCAAGCC 
CTGGACCTGT ACCATTGGCT CATGGTCCTC AAGATCTCAT TACCAGTCAT TGGGCTCGAC 
GAAATCCTCA AGTTCGTTGC TCGAAACTAC CTGGAGGATC CAGAAGATGA AAGAAGGAAG 
CAACCAGGCC TTTTGCCCTC CCTCCCCACC CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fast-twitch skeletal muscle fiber adaptation to SERCA1 deficiency in a Dutch Improved Red and White calf pseudomyotonia case.
Neuromuscular disorders : NMD25(11)888-97(2015 Nov)
Dorotea T,Gr?nberg W,Murgiano L,Plattet P,Dr?gem?ller C,Mascarello F,Sacchetto R

Inhibition of ubiquitin proteasome system rescues the defective sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1) protein causing Chianina cattle pseudomyotonia.
The Journal of biological chemistry289(48)33073-82(2014 Nov)
Bianchini E,Testoni S,Gentile A,Cal? T,Ottolini D,Villa A,Brini M,Betto R,Mascarello F,Nissen P,Sandon? D,Sacchetto R

Crystal structure of sarcoplasmic reticulum Ca2+-ATPase (SERCA) from bovine muscle.
Journal of structural biology178(1)38-44(2012 Apr)
Sacchetto R,Bertipaglia I,Giannetti S,Cendron L,Mascarello F,Damiani E,Carafoli E,Zanotti G

A defective SERCA1 protein is responsible for congenital pseudomyotonia in Chianina cattle.
The American journal of pathology174(2)565-73(2009 Feb)
Sacchetto R,Testoni S,Gentile A,Damiani E,Rossi M,Liguori R,Dr?gem?ller C,Mascarello F

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ATP2A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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