NEBL cDNA ORF clone, Bos taurus(cattle)

The following NEBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221627 XM_025000590.1
Latest version!
Bos taurus nebulette (NEBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa01427 NM_001191413.2
Latest version!
Bos taurus nebulette (NEBL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa25065 XM_005214191.4
Latest version!
Bos taurus nebulette (NEBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa25066 XM_005214192.4
Latest version!
Bos taurus nebulette (NEBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa49792 XM_025000589.1
Latest version!
Bos taurus nebulette (NEBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa92233 XM_015473988.1
Latest version!
Bos taurus nebulette (NEBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OBa25066 XM_005214192.3 Bos taurus nebulette (NEBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa25065 XM_005214191.3 Bos taurus nebulette (NEBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221627
    Clone ID Related Accession (Same CDS sequence) XM_025000590.1
    Accession Version XM_025000590.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulette isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
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    301
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    481
    541
    601
    661
    721
    781
    ATGAACCCCC AGTGCGCCCG CTGCGGGAAA GTCGTGTATC CCACCGAGAA AGTCAACTGC 
    TTGGATAAGT ACTGGCATAA AGGATGTTTC CATTGTGAAG TCTGCAAGAT GGCTCTCAAC
    ATGAACAACT ACAAAGGCTA TGAAAAGAAG CCCTATTGTA ATGCACACTA CCCAAAGCAG
    TCCTTCACCA CGGTGGCAGA TACACCTGAA AACCTTCGCC TGAAGCAGCA AAGTGAATTG
    CAGAGTCAGG TCAAGTACAA AAGAGATTTT GAAGAAAGCA AAGGGAGGGG CTTCAGCATC
    GTTACAGACA CTCCTGAACT ACAGAGGCTG AAGAGGACTC AGGAACAAAT CAGTAACGTG
    AAATATCATG AAGATTTTGA AAAGACAAAG GGAAGGGGCT TTACTCCTGT TGTGGATGAC
    CCTGTGACAG AGCGAGTGAG AAGGAACACC CAGGTCGTCA GCGATGCTGC CTATAAAGGT
    GTCCATCCTC ACATCGTGGA GATGGACAGG AGACCTGGAA TCATTGTTGC GCCTGTTCTT
    CCTGGAGCCT ACCAGCAAAG CCATTCTCAA GGCTATGGGT ACATGCACCA GACCAGCCTG
    TCATCTGTGA GGTCAATGCA GCATTCACCA AATCTAAGGA CCTACCGAGC CATGTACGAT
    TACAGTGCCC AGGATGAAGA TGAAGTCTCC TTCAGAGATG GGGACTACAT CGTCAATGTG
    CAGCCCATTG ACGACGGCTG GATGTACGGC ACGGTGCAGA GAACTGGAAA AACCGGAATG
    CTTCCAGCGA ATTACATTGA GTTTGTTAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856358.1
    CDS94..906
    Translation

    Target ORF information:

    RefSeq Version XM_025000590.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAACCCCC AGTGCGCCCG CTGCGGGAAA GTCGTGTATC CCACCGAGAA AGTCAACTGC 
    TTGGATAAGT ACTGGCATAA AGGATGTTTC CATTGTGAAG TCTGCAAGAT GGCTCTCAAC
    ATGAACAACT ACAAAGGCTA TGAAAAGAAG CCCTATTGTA ATGCACACTA CCCAAAGCAG
    TCCTTCACCA CGGTGGCAGA TACACCTGAA AACCTTCGCC TGAAGCAGCA AAGTGAATTG
    CAGAGTCAGG TCAAGTACAA AAGAGATTTT GAAGAAAGCA AAGGGAGGGG CTTCAGCATC
    GTTACAGACA CTCCTGAACT ACAGAGGCTG AAGAGGACTC AGGAACAAAT CAGTAACGTG
    AAATATCATG AAGATTTTGA AAAGACAAAG GGAAGGGGCT TTACTCCTGT TGTGGATGAC
    CCTGTGACAG AGCGAGTGAG AAGGAACACC CAGGTCGTCA GCGATGCTGC CTATAAAGGT
    GTCCATCCTC ACATCGTGGA GATGGACAGG AGACCTGGAA TCATTGTTGC GCCTGTTCTT
    CCTGGAGCCT ACCAGCAAAG CCATTCTCAA GGCTATGGGT ACATGCACCA GACCAGCCTG
    TCATCTGTGA GGTCAATGCA GCATTCACCA AATCTAAGGA CCTACCGAGC CATGTACGAT
    TACAGTGCCC AGGATGAAGA TGAAGTCTCC TTCAGAGATG GGGACTACAT CGTCAATGTG
    CAGCCCATTG ACGACGGCTG GATGTACGGC ACGGTGCAGA GAACTGGAAA AACCGGAATG
    CTTCCAGCGA ATTACATTGA GTTTGTTAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01427
    Clone ID Related Accession (Same CDS sequence) NM_001191413.2
    Accession Version NM_001191413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3042bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-10
    Organism Bos taurus(cattle)
    Product nebulette
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000013.1. On Apr 26, 2014 this sequence version replaced NM_001191413.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721697.210.1, SRR5721697.7690.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGACCTC
    AAAGTTTGGC GCACAGATCC TGGCTCCATC TTCGACATTG ATCCCCTGGA AGACAATATT
    CAGTCTAGGA GTCTACATAT GCTTTCTGAA AAGGCGAATC TTTATAGGAG ACAACGGTCT
    CGGTCTTACT CGAGCAGTAC TTTTGGTACA GGTCTGGGAG ATGACAAATC AGAAATATCT
    GAGATTTACC GTAGCTTTTC ATGCTGCAGT GAGGTAACAA GACCATCTGA TGAAGGAGCG
    CCTGTTCTTC CTGGAGCCTA CCAGCAAAGC CATTCTCAAG GCTATGGGTA CATGCACCAG
    ACCAGCCTGT CATCTGTGAG GTCAATGCAG CATTCACCAA ATCTAAGGAC CTACCGAGCC
    ATGTACGATT ACAGTGCCCA GGATGAAGAT GAAGTCTCCT TCAGAGATGG GGACTACATC
    GTCAATGTGC AGCCCATTGA CGACGGCTGG ATGTACGGCA CGGTGCAGAG AACTGGAAAA
    ACCGGAATGC TTCCAGCGAA TTACATTGAG TTTGTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178342.1
    CDS26..3067
    Translation

    Target ORF information:

    RefSeq Version NM_001191413.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGACCTC
    AAAGTTTGGC GCACAGATCC TGGCTCCATC TTCGACATTG ATCCCCTGGA AGACAATATT
    CAGTCTAGGA GTCTACATAT GCTTTCTGAA AAGGCGAATC TTTATAGGAG ACAACGGTCT
    CGGTCTTACT CGAGCAGTAC TTTTGGTACA GGTCTGGGAG ATGACAAATC AGAAATATCT
    GAGATTTACC GTAGCTTTTC ATGCTGCAGT GAGGTAACAA GACCATCTGA TGAAGGAGCG
    CCTGTTCTTC CTGGAGCCTA CCAGCAAAGC CATTCTCAAG GCTATGGGTA CATGCACCAG
    ACCAGCCTGT CATCTGTGAG GTCAATGCAG CATTCACCAA ATCTAAGGAC CTACCGAGCC
    ATGTACGATT ACAGTGCCCA GGATGAAGAT GAAGTCTCCT TCAGAGATGG GGACTACATC
    GTCAATGTGC AGCCCATTGA CGACGGCTGG ATGTACGGCA CGGTGCAGAG AACTGGAAAA
    ACCGGAATGC TTCCAGCGAA TTACATTGAG TTTGTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25065
    Clone ID Related Accession (Same CDS sequence) XM_005214191.3 , XM_005214191.4
    Accession Version XM_005214191.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulette isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATCG AGAATATAGA AAGGTTTTTG AGGAAAACAA AGGCCTGTAT
    CATTTTGATG TGGACGCTGC GGAACACCTG CACCATAAAG GCAATGCCAT GCTCCAGAGT
    CAGGTGAAGT ACAAAGAAGA GTATGAGAAA AATAAGGGGA AATCGATGCT TGAATTTGTT
    GAGACACCAT CATATCAAGT TTCAAAGGAG GCTCAGAAGA TGCAGAGTGA GAAAGTTTAC
    AGAGAGGATT TTGAGAAGGA TATTAAAGGA AGGTCATCAA TGGATTTAGA CAAGACTCCA
    GAGTTTTTAC ATGTAAAGTA CATCACCAAC CTTCTGAAGG AGAAAGAATA TAAAAAGGAT
    TTGGAAAATG AAATAAAAGG GAAAGGAATG GAGCTTAATT CCGAAGTACT TGATATTCAA
    AGAGCAAAAC GAGCATCTGA AATGACTAGT GAGAAAGAAT ACAAGAAAGA CCTGGAATCA
    GAAATTAAAG GGAAGGGAAT GCAAGTTGGC ACTGACACCC TTGGAATTCA GCATGCCAAA
    AGAGCTTCTG AGATGGCGAG TGAGAAAGAT TATAAAAGAG ATCTGGAGAC TGAAATTAGA
    GGGAAAGGCA TGCAGGTGAG CACAGACACT CTCGATATCC AGAGAGCCAA GAAAGCATCT
    GAAATGGCCA GCCAGAAAGA ATATAAGAAA GACTTAGAAA ATGAAATTAA AGGGAAAGGA
    ATGCAGGTGA GCATGGATAT CCCGGATATG CTTCGAGCCA AGAGGGCATC TGAAATCTAT
    AGCCAGAAAA AGTATAAAGA TGAGGCAGAG AAGATGCTTT CTAGCTATTC TACTGTGGCA
    GATACTCCTG AAATTCAGAG AATTAAGACA ACTCAACAAA ACATTAGTGC GGTATTTTAT
    AAGAAAGAAG TAGGAGCTGG CACAGCAGTA AAAGATACTC CTGAGATTGA ACGAGTGAAG
    AAAAATCAGC AGAACATTAG TTCAGTAAAA TACAAAGAAG AGGTGAAACA TGCAACAGCC
    ATTTCAGATC CACCAGAACT CAAGAGAGTT AAAGAAAACC AGAAGAACAT CAGCAATCTC
    AGGTACAAAG AGCCGTGTGA CAGAGCAACT CCTGTGAGCA TGACGCCCGA GATAGAGAGA
    GTGAGGAGAA ACCAGGAGCA GCTGAGCACG GTAAAATACA GAGGAGAGAT TAAACAGGCG
    ACTGCAATTT CTGATCCACC AGAGCTGAAG AGAGTGAAAG AAAACCAGAA GAACCTCAGC
    AATGTTTATT ATAAATCTCA GATGGGAAGA GCTACAGCTT TAAGTGTAAC TCCTGAAATG
    GAAAGAGTAA AGAAGAATCA AGAAAATATT AGTTCGGTAA AATATACCCA GGACCAGAAA
    CAGATGAAAG GTAGACCAAG TCTGATTTTA GATACACCTG GTCTCAGACA TGTCAAAGAA
    GCTCAAAATC GTATTTCAAT GGTGAAATAT CATGAAGATT TTGAAAAGAC AAAGGGAAGG
    GGCTTTACTC CTGTTGTGGA TGACCCTGTG ACAGAGCGAG TGAGAAGGAA CACCCAGGTC
    GTCAGCGATG CTGCCTATAA AGGTGTCCAT CCTCACATCG TGGAGATGGA CAGGAGACCT
    GGAATCATTG TTGACCTCAA AGTTTGGCGC ACAGATCCTG GCTCCATCTT CGACATTGAT
    CCCCTGGAAG ACAATATTCA GTCTAGGAGT CTACATATGC TTTCTGAAAA GGCGAATCTT
    TATAGGAGAC AACGGTCTCG GTCTTACTCG AGCAGTACTT TTGGTACAGG TCTGGGAGAT
    GACAAATCAG AAATATCTGA GATTTACCGT AGCTTTTCAT GCTGCAGTGA GGTAACAAGA
    CCATCTGATG AAGGAGCGCC TGTTCTTCCT GGAGCCTACC AGCAAAGCCA TTCTCAAGGC
    TATGGGTACA TGCACCAGAC CAGCCTGTCA TCTGTGAGGT CAATGCAGCA TTCACCAAAT
    CTAAGGACCT ACCGAGCCAT GTACGATTAC AGTGCCCAGG ATGAAGATGA AGTCTCCTTC
    AGAGATGGGG ACTACATCGT CAATGTGCAG CCCATTGACG ACGGCTGGAT GTACGGCACG
    GTGCAGAGAA CTGGAAAAAC CGGAATGCTT CCAGCGAATT ACATTGAGTT TGTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214248.1
    CDS102..3041
    Translation

    Target ORF information:

    RefSeq Version XM_005214191.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214191.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATCG AGAATATAGA AAGGTTTTTG AGGAAAACAA AGGCCTGTAT
    CATTTTGATG TGGACGCTGC GGAACACCTG CACCATAAAG GCAATGCCAT GCTCCAGAGT
    CAGGTGAAGT ACAAAGAAGA GTATGAGAAA AATAAGGGGA AATCGATGCT TGAATTTGTT
    GAGACACCAT CATATCAAGT TTCAAAGGAG GCTCAGAAGA TGCAGAGTGA GAAAGTTTAC
    AGAGAGGATT TTGAGAAGGA TATTAAAGGA AGGTCATCAA TGGATTTAGA CAAGACTCCA
    GAGTTTTTAC ATGTAAAGTA CATCACCAAC CTTCTGAAGG AGAAAGAATA TAAAAAGGAT
    TTGGAAAATG AAATAAAAGG GAAAGGAATG GAGCTTAATT CCGAAGTACT TGATATTCAA
    AGAGCAAAAC GAGCATCTGA AATGACTAGT GAGAAAGAAT ACAAGAAAGA CCTGGAATCA
    GAAATTAAAG GGAAGGGAAT GCAAGTTGGC ACTGACACCC TTGGAATTCA GCATGCCAAA
    AGAGCTTCTG AGATGGCGAG TGAGAAAGAT TATAAAAGAG ATCTGGAGAC TGAAATTAGA
    GGGAAAGGCA TGCAGGTGAG CACAGACACT CTCGATATCC AGAGAGCCAA GAAAGCATCT
    GAAATGGCCA GCCAGAAAGA ATATAAGAAA GACTTAGAAA ATGAAATTAA AGGGAAAGGA
    ATGCAGGTGA GCATGGATAT CCCGGATATG CTTCGAGCCA AGAGGGCATC TGAAATCTAT
    AGCCAGAAAA AGTATAAAGA TGAGGCAGAG AAGATGCTTT CTAGCTATTC TACTGTGGCA
    GATACTCCTG AAATTCAGAG AATTAAGACA ACTCAACAAA ACATTAGTGC GGTATTTTAT
    AAGAAAGAAG TAGGAGCTGG CACAGCAGTA AAAGATACTC CTGAGATTGA ACGAGTGAAG
    AAAAATCAGC AGAACATTAG TTCAGTAAAA TACAAAGAAG AGGTGAAACA TGCAACAGCC
    ATTTCAGATC CACCAGAACT CAAGAGAGTT AAAGAAAACC AGAAGAACAT CAGCAATCTC
    AGGTACAAAG AGCCGTGTGA CAGAGCAACT CCTGTGAGCA TGACGCCCGA GATAGAGAGA
    GTGAGGAGAA ACCAGGAGCA GCTGAGCACG GTAAAATACA GAGGAGAGAT TAAACAGGCG
    ACTGCAATTT CTGATCCACC AGAGCTGAAG AGAGTGAAAG AAAACCAGAA GAACCTCAGC
    AATGTTTATT ATAAATCTCA GATGGGAAGA GCTACAGCTT TAAGTGTAAC TCCTGAAATG
    GAAAGAGTAA AGAAGAATCA AGAAAATATT AGTTCGGTAA AATATACCCA GGACCAGAAA
    CAGATGAAAG GTAGACCAAG TCTGATTTTA GATACACCTG GTCTCAGACA TGTCAAAGAA
    GCTCAAAATC GTATTTCAAT GGTGAAATAT CATGAAGATT TTGAAAAGAC AAAGGGAAGG
    GGCTTTACTC CTGTTGTGGA TGACCCTGTG ACAGAGCGAG TGAGAAGGAA CACCCAGGTC
    GTCAGCGATG CTGCCTATAA AGGTGTCCAT CCTCACATCG TGGAGATGGA CAGGAGACCT
    GGAATCATTG TTGACCTCAA AGTTTGGCGC ACAGATCCTG GCTCCATCTT CGACATTGAT
    CCCCTGGAAG ACAATATTCA GTCTAGGAGT CTACATATGC TTTCTGAAAA GGCGAATCTT
    TATAGGAGAC AACGGTCTCG GTCTTACTCG AGCAGTACTT TTGGTACAGG TCTGGGAGAT
    GACAAATCAG AAATATCTGA GATTTACCGT AGCTTTTCAT GCTGCAGTGA GGTAACAAGA
    CCATCTGATG AAGGAGCGCC TGTTCTTCCT GGAGCCTACC AGCAAAGCCA TTCTCAAGGC
    TATGGGTACA TGCACCAGAC CAGCCTGTCA TCTGTGAGGT CAATGCAGCA TTCACCAAAT
    CTAAGGACCT ACCGAGCCAT GTACGATTAC AGTGCCCAGG ATGAAGATGA AGTCTCCTTC
    AGAGATGGGG ACTACATCGT CAATGTGCAG CCCATTGACG ACGGCTGGAT GTACGGCACG
    GTGCAGAGAA CTGGAAAAAC CGGAATGCTT CCAGCGAATT ACATTGAGTT TGTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25065
    Clone ID Related Accession (Same CDS sequence) XM_005214191.3 , XM_005214191.4
    Accession Version XM_005214191.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulette isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214191.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATCG AGAATATAGA AAGGTTTTTG AGGAAAACAA AGGCCTGTAT
    CATTTTGATG TGGACGCTGC GGAACACCTG CACCATAAAG GCAATGCCAT GCTCCAGAGT
    CAGGTGAAGT ACAAAGAAGA GTATGAGAAA AATAAGGGGA AATCGATGCT TGAATTTGTT
    GAGACACCAT CATATCAAGT TTCAAAGGAG GCTCAGAAGA TGCAGAGTGA GAAAGTTTAC
    AGAGAGGATT TTGAGAAGGA TATTAAAGGA AGGTCATCAA TGGATTTAGA CAAGACTCCA
    GAGTTTTTAC ATGTAAAGTA CATCACCAAC CTTCTGAAGG AGAAAGAATA TAAAAAGGAT
    TTGGAAAATG AAATAAAAGG GAAAGGAATG GAGCTTAATT CCGAAGTACT TGATATTCAA
    AGAGCAAAAC GAGCATCTGA AATGACTAGT GAGAAAGAAT ACAAGAAAGA CCTGGAATCA
    GAAATTAAAG GGAAGGGAAT GCAAGTTGGC ACTGACACCC TTGGAATTCA GCATGCCAAA
    AGAGCTTCTG AGATGGCGAG TGAGAAAGAT TATAAAAGAG ATCTGGAGAC TGAAATTAGA
    GGGAAAGGCA TGCAGGTGAG CACAGACACT CTCGATATCC AGAGAGCCAA GAAAGCATCT
    GAAATGGCCA GCCAGAAAGA ATATAAGAAA GACTTAGAAA ATGAAATTAA AGGGAAAGGA
    ATGCAGGTGA GCATGGATAT CCCGGATATG CTTCGAGCCA AGAGGGCATC TGAAATCTAT
    AGCCAGAAAA AGTATAAAGA TGAGGCAGAG AAGATGCTTT CTAGCTATTC TACTGTGGCA
    GATACTCCTG AAATTCAGAG AATTAAGACA ACTCAACAAA ACATTAGTGC GGTATTTTAT
    AAGAAAGAAG TAGGAGCTGG CACAGCAGTA AAAGATACTC CTGAGATTGA ACGAGTGAAG
    AAAAATCAGC AGAACATTAG TTCAGTAAAA TACAAAGAAG AGGTGAAACA TGCAACAGCC
    ATTTCAGATC CACCAGAACT CAAGAGAGTT AAAGAAAACC AGAAGAACAT CAGCAATCTC
    AGGTACAAAG AGCCGTGTGA CAGAGCAACT CCTGTGAGCA TGACGCCCGA GATAGAGAGA
    GTGAGGAGAA ACCAGGAGCA GCTGAGCACG GTAAAATACA GAGGAGAGAT TAAACAGGCG
    ACTGCAATTT CTGATCCACC AGAGCTGAAG AGAGTGAAAG AAAACCAGAA GAACCTCAGC
    AATGTTTATT ATAAATCTCA GATGGGAAGA GCTACAGCTT TAAGTGTAAC TCCTGAAATG
    GAAAGAGTAA AGAAGAATCA AGAAAATATT AGTTCGGTAA AATATACCCA GGACCAGAAA
    CAGATGAAAG GTAGACCAAG TCTGATTTTA GATACACCTG GTCTCAGACA TGTCAAAGAA
    GCTCAAAATC GTATTTCAAT GGTGAAATAT CATGAAGATT TTGAAAAGAC AAAGGGAAGG
    GGCTTTACTC CTGTTGTGGA TGACCCTGTG ACAGAGCGAG TGAGAAGGAA CACCCAGGTC
    GTCAGCGATG CTGCCTATAA AGGTGTCCAT CCTCACATCG TGGAGATGGA CAGGAGACCT
    GGAATCATTG TTGACCTCAA AGTTTGGCGC ACAGATCCTG GCTCCATCTT CGACATTGAT
    CCCCTGGAAG ACAATATTCA GTCTAGGAGT CTACATATGC TTTCTGAAAA GGCGAATCTT
    TATAGGAGAC AACGGTCTCG GTCTTACTCG AGCAGTACTT TTGGTACAGG TCTGGGAGAT
    GACAAATCAG AAATATCTGA GATTTACCGT AGCTTTTCAT GCTGCAGTGA GGTAACAAGA
    CCATCTGATG AAGGAGCGCC TGTTCTTCCT GGAGCCTACC AGCAAAGCCA TTCTCAAGGC
    TATGGGTACA TGCACCAGAC CAGCCTGTCA TCTGTGAGGT CAATGCAGCA TTCACCAAAT
    CTAAGGACCT ACCGAGCCAT GTACGATTAC AGTGCCCAGG ATGAAGATGA AGTCTCCTTC
    AGAGATGGGG ACTACATCGT CAATGTGCAG CCCATTGACG ACGGCTGGAT GTACGGCACG
    GTGCAGAGAA CTGGAAAAAC CGGAATGCTT CCAGCGAATT ACATTGAGTT TGTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214248.1
    CDS110..3049
    Translation

    Target ORF information:

    RefSeq Version XM_005214191.4
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214191.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATCG AGAATATAGA AAGGTTTTTG AGGAAAACAA AGGCCTGTAT
    CATTTTGATG TGGACGCTGC GGAACACCTG CACCATAAAG GCAATGCCAT GCTCCAGAGT
    CAGGTGAAGT ACAAAGAAGA GTATGAGAAA AATAAGGGGA AATCGATGCT TGAATTTGTT
    GAGACACCAT CATATCAAGT TTCAAAGGAG GCTCAGAAGA TGCAGAGTGA GAAAGTTTAC
    AGAGAGGATT TTGAGAAGGA TATTAAAGGA AGGTCATCAA TGGATTTAGA CAAGACTCCA
    GAGTTTTTAC ATGTAAAGTA CATCACCAAC CTTCTGAAGG AGAAAGAATA TAAAAAGGAT
    TTGGAAAATG AAATAAAAGG GAAAGGAATG GAGCTTAATT CCGAAGTACT TGATATTCAA
    AGAGCAAAAC GAGCATCTGA AATGACTAGT GAGAAAGAAT ACAAGAAAGA CCTGGAATCA
    GAAATTAAAG GGAAGGGAAT GCAAGTTGGC ACTGACACCC TTGGAATTCA GCATGCCAAA
    AGAGCTTCTG AGATGGCGAG TGAGAAAGAT TATAAAAGAG ATCTGGAGAC TGAAATTAGA
    GGGAAAGGCA TGCAGGTGAG CACAGACACT CTCGATATCC AGAGAGCCAA GAAAGCATCT
    GAAATGGCCA GCCAGAAAGA ATATAAGAAA GACTTAGAAA ATGAAATTAA AGGGAAAGGA
    ATGCAGGTGA GCATGGATAT CCCGGATATG CTTCGAGCCA AGAGGGCATC TGAAATCTAT
    AGCCAGAAAA AGTATAAAGA TGAGGCAGAG AAGATGCTTT CTAGCTATTC TACTGTGGCA
    GATACTCCTG AAATTCAGAG AATTAAGACA ACTCAACAAA ACATTAGTGC GGTATTTTAT
    AAGAAAGAAG TAGGAGCTGG CACAGCAGTA AAAGATACTC CTGAGATTGA ACGAGTGAAG
    AAAAATCAGC AGAACATTAG TTCAGTAAAA TACAAAGAAG AGGTGAAACA TGCAACAGCC
    ATTTCAGATC CACCAGAACT CAAGAGAGTT AAAGAAAACC AGAAGAACAT CAGCAATCTC
    AGGTACAAAG AGCCGTGTGA CAGAGCAACT CCTGTGAGCA TGACGCCCGA GATAGAGAGA
    GTGAGGAGAA ACCAGGAGCA GCTGAGCACG GTAAAATACA GAGGAGAGAT TAAACAGGCG
    ACTGCAATTT CTGATCCACC AGAGCTGAAG AGAGTGAAAG AAAACCAGAA GAACCTCAGC
    AATGTTTATT ATAAATCTCA GATGGGAAGA GCTACAGCTT TAAGTGTAAC TCCTGAAATG
    GAAAGAGTAA AGAAGAATCA AGAAAATATT AGTTCGGTAA AATATACCCA GGACCAGAAA
    CAGATGAAAG GTAGACCAAG TCTGATTTTA GATACACCTG GTCTCAGACA TGTCAAAGAA
    GCTCAAAATC GTATTTCAAT GGTGAAATAT CATGAAGATT TTGAAAAGAC AAAGGGAAGG
    GGCTTTACTC CTGTTGTGGA TGACCCTGTG ACAGAGCGAG TGAGAAGGAA CACCCAGGTC
    GTCAGCGATG CTGCCTATAA AGGTGTCCAT CCTCACATCG TGGAGATGGA CAGGAGACCT
    GGAATCATTG TTGACCTCAA AGTTTGGCGC ACAGATCCTG GCTCCATCTT CGACATTGAT
    CCCCTGGAAG ACAATATTCA GTCTAGGAGT CTACATATGC TTTCTGAAAA GGCGAATCTT
    TATAGGAGAC AACGGTCTCG GTCTTACTCG AGCAGTACTT TTGGTACAGG TCTGGGAGAT
    GACAAATCAG AAATATCTGA GATTTACCGT AGCTTTTCAT GCTGCAGTGA GGTAACAAGA
    CCATCTGATG AAGGAGCGCC TGTTCTTCCT GGAGCCTACC AGCAAAGCCA TTCTCAAGGC
    TATGGGTACA TGCACCAGAC CAGCCTGTCA TCTGTGAGGT CAATGCAGCA TTCACCAAAT
    CTAAGGACCT ACCGAGCCAT GTACGATTAC AGTGCCCAGG ATGAAGATGA AGTCTCCTTC
    AGAGATGGGG ACTACATCGT CAATGTGCAG CCCATTGACG ACGGCTGGAT GTACGGCACG
    GTGCAGAGAA CTGGAAAAAC CGGAATGCTT CCAGCGAATT ACATTGAGTT TGTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25066
    Clone ID Related Accession (Same CDS sequence) XM_005214192.4 , XM_005214192.3
    Accession Version XM_005214192.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulette isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214192.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGCGCCT
    GTTCTTCCTG GAGCCTACCA GCAAAGCCAT TCTCAAGGCT ATGGGTACAT GCACCAGACC
    AGCCTGTCAT CTGTGAGGTC AATGCAGCAT TCACCAAATC TAAGGACCTA CCGAGCCATG
    TACGATTACA GTGCCCAGGA TGAAGATGAA GTCTCCTTCA GAGATGGGGA CTACATCGTC
    AATGTGCAGC CCATTGACGA CGGCTGGATG TACGGCACGG TGCAGAGAAC TGGAAAAACC
    GGAATGCTTC CAGCGAATTA CATTGAGTTT GTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214249.1
    CDS109..2907
    Translation

    Target ORF information:

    RefSeq Version XM_005214192.4
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214192.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGCGCCT
    GTTCTTCCTG GAGCCTACCA GCAAAGCCAT TCTCAAGGCT ATGGGTACAT GCACCAGACC
    AGCCTGTCAT CTGTGAGGTC AATGCAGCAT TCACCAAATC TAAGGACCTA CCGAGCCATG
    TACGATTACA GTGCCCAGGA TGAAGATGAA GTCTCCTTCA GAGATGGGGA CTACATCGTC
    AATGTGCAGC CCATTGACGA CGGCTGGATG TACGGCACGG TGCAGAGAAC TGGAAAAACC
    GGAATGCTTC CAGCGAATTA CATTGAGTTT GTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25066
    Clone ID Related Accession (Same CDS sequence) XM_005214192.4 , XM_005214192.3
    Accession Version XM_005214192.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulette isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGCGCCT
    GTTCTTCCTG GAGCCTACCA GCAAAGCCAT TCTCAAGGCT ATGGGTACAT GCACCAGACC
    AGCCTGTCAT CTGTGAGGTC AATGCAGCAT TCACCAAATC TAAGGACCTA CCGAGCCATG
    TACGATTACA GTGCCCAGGA TGAAGATGAA GTCTCCTTCA GAGATGGGGA CTACATCGTC
    AATGTGCAGC CCATTGACGA CGGCTGGATG TACGGCACGG TGCAGAGAAC TGGAAAAACC
    GGAATGCTTC CAGCGAATTA CATTGAGTTT GTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214249.1
    CDS102..2900
    Translation

    Target ORF information:

    RefSeq Version XM_005214192.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAAATTAAA TACAAAGAAA AATTTGATAG TGAAATGAAG
    GATAAGAAAC ATCTTTACAA TCCTCTTGAA AGTGCTTCTT TTAGGCAAAG TCAGCTGGCC
    ACTGCCCTGG CGAGCAATGT GAAGTACAAG AAAGACATTC AGAATATGCA TGATCCAGTT
    TCAGGTCTCC CAAACTTGTT CTTAGACCAC GCTTTGAAAG TCAGCAAAAT GCTAAGCGGA
    CGAGAATATA GAAAGGTTTT TGAGGAAAAC AAAGGCCTGT ATCATTTTGA TGTGGACGCT
    GCGGAACACC TGCACCATAA AGGCAATGCC ATGCTCCAGA GTCAGGTGAA GTACAAAGAA
    GAGTATGAGA AAAATAAGGG GAAATCGATG CTTGAATTTG TTGAGACACC ATCATATCAA
    GTTTCAAAGG AGGCTCAGAA GATGCAGAGT GAGAAAGTTT ACAGAGAGGA TTTTGAGAAG
    GATATTAAAG GAAGGTCATC AATGGATTTA GACAAGACTC CAGAGTTTTT ACATGTAAAG
    TACATCACCA ACCTTCTGAA GGAGAAAGAA TATAAAAAGG ATTTGGAAAA TGAAATAAAA
    GGGAAAGGAA TGGAGCTTAA TTCCGAAGTA CTTGATATTC AAAGAGCAAA ACGAGCATCT
    GAAATGACTA GTGAGAAAGA ATACAAGAAA GACCTGGAAT CAGAAATTAA AGGGAAGGGA
    ATGCAAGTTG GCACTGACAC CCTTGGAATT CAGCATGCCA AAAGAGCTTC TGAGATGGCG
    AGTGAGAAAG ATTATAAAAG AGATCTGGAG ACTGAAATTA GAGGGAAAGG CATGCAGGTG
    AGCACAGACA CTCTCGATAT CCAGAGAGCC AAGAAAGCAT CTGAAATGGC CAGCCAGAAA
    GAATATAAGA AAGACTTAGA AAATGAAATT AAAGGGAAAG GAATGCAGGT GAGCATGGAT
    ATCCCGGATA TGCTTCGAGC CAAGAGGGCA TCTGAAATCT ATAGCCAGAA AAAGTATAAA
    GATGAGGCAG AGAAGATGCT TTCTAGCTAT TCTACTGTGG CAGATACTCC TGAAATTCAG
    AGAATTAAGA CAACTCAACA AAACATTAGT GCGGTATTTT ATAAGAAAGA AGTAGGAGCT
    GGCACAGCAG TAAAAGATAC TCCTGAGATT GAACGAGTGA AGAAAAATCA GCAGAACATT
    AGTTCAGTAA AATACAAAGA AGAGGTGAAA CATGCAACAG CCATTTCAGA TCCACCAGAA
    CTCAAGAGAG TTAAAGAAAA CCAGAAGAAC ATCAGCAATC TCAGGTACAA AGAGCCGTGT
    GACAGAGCAA CTCCTGTGAG CATGACGCCC GAGATAGAGA GAGTGAGGAG AAACCAGGAG
    CAGCTGAGCA CGGTAAAATA CAGAGGAGAG ATTAAACAGG CGACTGCAAT TTCTGATCCA
    CCAGAGCTGA AGAGAGTGAA AGAAAACCAG AAGAACCTCA GCAATGTTTA TTATAAATCT
    CAGATGGGAA GAGCTACAGC TTTAAGTGTA ACTCCTGAAA TGGAAAGAGT AAAGAAGAAT
    CAAGAAAATA TTAGTTCGGT AAAATATACC CAGGACCAGA AACAGATGAA AGGTAGACCA
    AGTCTGATTT TAGATACACC TGGTCTCAGA CATGTCAAAG AAGCTCAAAA TCGTATTTCA
    ATGGTGAAAT ATCATGAAGA TTTTGAAAAG ACAAAGGGAA GGGGCTTTAC TCCTGTTGTG
    GATGACCCTG TGACAGAGCG AGTGAGAAGG AACACCCAGG TCGTCAGCGA TGCTGCCTAT
    AAAGGTGTCC ATCCTCACAT CGTGGAGATG GACAGGAGAC CTGGAATCAT TGTTGCGCCT
    GTTCTTCCTG GAGCCTACCA GCAAAGCCAT TCTCAAGGCT ATGGGTACAT GCACCAGACC
    AGCCTGTCAT CTGTGAGGTC AATGCAGCAT TCACCAAATC TAAGGACCTA CCGAGCCATG
    TACGATTACA GTGCCCAGGA TGAAGATGAA GTCTCCTTCA GAGATGGGGA CTACATCGTC
    AATGTGCAGC CCATTGACGA CGGCTGGATG TACGGCACGG TGCAGAGAAC TGGAAAAACC
    GGAATGCTTC CAGCGAATTA CATTGAGTTT GTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49792
    Clone ID Related Accession (Same CDS sequence) XM_025000589.1
    Accession Version XM_025000589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulette isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAACGAGAA TATAGAAAGG TTTTTGAGGA AAACAAAGGC
    CTGTATCATT TTGATGTGGA CGCTGCGGAA CACCTGCACC ATAAAGGCAA TGCCATGCTC
    CAGAGTCAGG TGAAGTACAA AGAAGAGTAT GAGAAAAATA AGGGGAAATC GATGCTTGAA
    TTTGTTGAGA CACCATCATA TCAAGTTTCA AAGGAGGCTC AGAAGATGCA GAGTGAGAAA
    GTTTACAGAG AGGATTTTGA GAAGGATATT AAAGGAAGGT CATCAATGGA TTTAGACAAG
    ACTCCAGAGT TTTTACATGT AAAGTACATC ACCAACCTTC TGAAGGAGAA AGAATATAAA
    AAGGATTTGG AAAATGAAAT AAAAGGGAAA GGAATGGAGC TTAATTCCGA AGTACTTGAT
    ATTCAAAGAG CAAAACGAGC ATCTGAAATG ACTAGTGAGA AAGAATACAA GAAAGACCTG
    GAATCAGAAA TTAAAGGGAA GGGAATGCAA GTTGGCACTG ACACCCTTGG AATTCAGCAT
    GCCAAAAGAG CTTCTGAGAT GGCGAGTGAG AAAGATTATA AAAGAGATCT GGAGACTGAA
    ATTAGAGGGA AAGGCATGCA GGTGAGCACA GACACTCTCG ATATCCAGAG AGCCAAGAAA
    GCATCTGAAA TGGCCAGCCA GAAAGAATAT AAGAAAGACT TAGAAAATGA AATTAAAGGG
    AAAGGAATGC AGGTGAGCAT GGATATCCCG GATATGCTTC GAGCCAAGAG GGCATCTGAA
    ATCTATAGCC AGAAAAAGTA TAAAGATGAG GCAGAGAAGA TGCTTTCTAG CTATTCTACT
    GTGGCAGATA CTCCTGAAAT TCAGAGAATT AAGACAACTC AACAAAACAT TAGTGCGGTA
    TTTTATAAGA AAGAAGTAGG AGCTGGCACA GCAGTAAAAG ATACTCCTGA GATTGAACGA
    GTGAAGAAAA ATCAGCAGAA CATTAGTTCA GTAAAATACA AAGAAGAGGT GAAACATGCA
    ACAGCCATTT CAGATCCACC AGAACTCAAG AGAGTTAAAG AAAACCAGAA GAACATCAGC
    AATCTCAGGT ACAAAGAGCC GTGTGACAGA GCAACTCCTG TGAGCATGAC GCCCGAGATA
    GAGAGAGTGA GGAGAAACCA GGAGCAGCTG AGCACGGTAA AATACAGAGG AGAGATTAAA
    CAGGCGACTG CAATTTCTGA TCCACCAGAG CTGAAGAGAG TGAAAGAAAA CCAGAAGAAC
    CTCAGCAATG TTTATTATAA ATCTCAGATG GGAAGAGCTA CAGCTTTAAG TGTAACTCCT
    GAAATGGAAA GAGTAAAGAA GAATCAAGAA AATATTAGTT CGGTAAAATA TACCCAGGAC
    CAGAAACAGA TGAAAGGTAG ACCAAGTCTG ATTTTAGATA CACCTGGTCT CAGACATGTC
    AAAGAAGCTC AAAATCGTAT TTCAATGGTG AAATATCATG AAGATTTTGA AAAGACAAAG
    GGAAGGGGCT TTACTCCTGT TGTGGATGAC CCTGTGACAG AGCGAGTGAG AAGGAACACC
    CAGGTCGTCA GCGATGCTGC CTATAAAGGT GTCCATCCTC ACATCGTGGA GATGGACAGG
    AGACCTGGAA TCATTGTTGA CCTCAAAGTT TGGCGCACAG ATCCTGGCTC CATCTTCGAC
    ATTGATCCCC TGGAAGACAA TATTCAGTCT AGGAGTCTAC ATATGCTTTC TGAAAAGGCG
    AATCTTTATA GGAGACAACG GTCTCGGTCT TACTCGAGCA GTACTTTTGG TACAGGTCTG
    GGAGATGACA AATCAGAAAT ATCTGAGATT TACCGTAGCT TTTCATGCTG CAGTGAGGTA
    ACAAGACCAT CTGATGAAGG AGCGCCTGTT CTTCCTGGAG CCTACCAGCA AAGCCATTCT
    CAAGGCTATG GGTACATGCA CCAGACCAGC CTGTCATCTG TGAGGTCAAT GCAGCATTCA
    CCAAATCTAA GGACCTACCG AGCCATGTAC GATTACAGTG CCCAGGATGA AGATGAAGTC
    TCCTTCAGAG ATGGGGACTA CATCGTCAAT GTGCAGCCCA TTGACGACGG CTGGATGTAC
    GGCACGGTGC AGAGAACTGG AAAAACCGGA ATGCTTCCAG CGAATTACAT TGAGTTTGTT
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856357.1
    CDS110..2935
    Translation

    Target ORF information:

    RefSeq Version XM_025000589.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTTC CTGTATTTGA GGATGTGAAA GATGAAACTG AAGAAGAAAA AACAGGGGAA 
    GAAGAAAATG AAGAAGACAA GGTATTCTTT AAGCCTGTTA TTGAAGACCT GAGTATGGAG
    TTGGCCAGAA GGTGCACTGA ACTCATTAGT GATATCCATT ATAAAGAAGA GTATAAAAAG
    TCTAAAGGCA AGTGTATGTT TGTGACTGAC ACGCCTATGC TAAACCATGT AAAACACATT
    GGTGCTTTTA TTTCTGAGGC AAAATACAAA GGCACCATTA AGGCGGACCT TTCCAATTCT
    CTTTATAAGC AGATGCCAGC CACAATTGAC AGTGTCTTTG CCAGAGAAGT CACACAACTA
    CAGAGTGAGA TTGCCTACAG GCAGAAGCAT GATGCGGCCA AAGGAGTCTC AGATTATGCC
    CACATGAAGG AGCCGCCTGA GATCAAACAT GCCATGGAGG TCAATAAGCA CCAGAGTAAT
    ATTTTTTATA GAAAAGATGT ACAGGACACC CACATGTATA ATGCAGAGCT TGACAGACCA
    GACATCAAGA TGGCGACACA GATCTCCAAG ATCATAAGCG ATGCTGAATA CAAGAAAGGG
    CAAGGAGTAA TGAATAAAGA GCCTGCTGTA ATTGGAAGAC CAGATTTTGA ACATGCTGTG
    GAAGCTTCTA AACTTTCTAG TCAACGAGAA TATAGAAAGG TTTTTGAGGA AAACAAAGGC
    CTGTATCATT TTGATGTGGA CGCTGCGGAA CACCTGCACC ATAAAGGCAA TGCCATGCTC
    CAGAGTCAGG TGAAGTACAA AGAAGAGTAT GAGAAAAATA AGGGGAAATC GATGCTTGAA
    TTTGTTGAGA CACCATCATA TCAAGTTTCA AAGGAGGCTC AGAAGATGCA GAGTGAGAAA
    GTTTACAGAG AGGATTTTGA GAAGGATATT AAAGGAAGGT CATCAATGGA TTTAGACAAG
    ACTCCAGAGT TTTTACATGT AAAGTACATC ACCAACCTTC TGAAGGAGAA AGAATATAAA
    AAGGATTTGG AAAATGAAAT AAAAGGGAAA GGAATGGAGC TTAATTCCGA AGTACTTGAT
    ATTCAAAGAG CAAAACGAGC ATCTGAAATG ACTAGTGAGA AAGAATACAA GAAAGACCTG
    GAATCAGAAA TTAAAGGGAA GGGAATGCAA GTTGGCACTG ACACCCTTGG AATTCAGCAT
    GCCAAAAGAG CTTCTGAGAT GGCGAGTGAG AAAGATTATA AAAGAGATCT GGAGACTGAA
    ATTAGAGGGA AAGGCATGCA GGTGAGCACA GACACTCTCG ATATCCAGAG AGCCAAGAAA
    GCATCTGAAA TGGCCAGCCA GAAAGAATAT AAGAAAGACT TAGAAAATGA AATTAAAGGG
    AAAGGAATGC AGGTGAGCAT GGATATCCCG GATATGCTTC GAGCCAAGAG GGCATCTGAA
    ATCTATAGCC AGAAAAAGTA TAAAGATGAG GCAGAGAAGA TGCTTTCTAG CTATTCTACT
    GTGGCAGATA CTCCTGAAAT TCAGAGAATT AAGACAACTC AACAAAACAT TAGTGCGGTA
    TTTTATAAGA AAGAAGTAGG AGCTGGCACA GCAGTAAAAG ATACTCCTGA GATTGAACGA
    GTGAAGAAAA ATCAGCAGAA CATTAGTTCA GTAAAATACA AAGAAGAGGT GAAACATGCA
    ACAGCCATTT CAGATCCACC AGAACTCAAG AGAGTTAAAG AAAACCAGAA GAACATCAGC
    AATCTCAGGT ACAAAGAGCC GTGTGACAGA GCAACTCCTG TGAGCATGAC GCCCGAGATA
    GAGAGAGTGA GGAGAAACCA GGAGCAGCTG AGCACGGTAA AATACAGAGG AGAGATTAAA
    CAGGCGACTG CAATTTCTGA TCCACCAGAG CTGAAGAGAG TGAAAGAAAA CCAGAAGAAC
    CTCAGCAATG TTTATTATAA ATCTCAGATG GGAAGAGCTA CAGCTTTAAG TGTAACTCCT
    GAAATGGAAA GAGTAAAGAA GAATCAAGAA AATATTAGTT CGGTAAAATA TACCCAGGAC
    CAGAAACAGA TGAAAGGTAG ACCAAGTCTG ATTTTAGATA CACCTGGTCT CAGACATGTC
    AAAGAAGCTC AAAATCGTAT TTCAATGGTG AAATATCATG AAGATTTTGA AAAGACAAAG
    GGAAGGGGCT TTACTCCTGT TGTGGATGAC CCTGTGACAG AGCGAGTGAG AAGGAACACC
    CAGGTCGTCA GCGATGCTGC CTATAAAGGT GTCCATCCTC ACATCGTGGA GATGGACAGG
    AGACCTGGAA TCATTGTTGA CCTCAAAGTT TGGCGCACAG ATCCTGGCTC CATCTTCGAC
    ATTGATCCCC TGGAAGACAA TATTCAGTCT AGGAGTCTAC ATATGCTTTC TGAAAAGGCG
    AATCTTTATA GGAGACAACG GTCTCGGTCT TACTCGAGCA GTACTTTTGG TACAGGTCTG
    GGAGATGACA AATCAGAAAT ATCTGAGATT TACCGTAGCT TTTCATGCTG CAGTGAGGTA
    ACAAGACCAT CTGATGAAGG AGCGCCTGTT CTTCCTGGAG CCTACCAGCA AAGCCATTCT
    CAAGGCTATG GGTACATGCA CCAGACCAGC CTGTCATCTG TGAGGTCAAT GCAGCATTCA
    CCAAATCTAA GGACCTACCG AGCCATGTAC GATTACAGTG CCCAGGATGA AGATGAAGTC
    TCCTTCAGAG ATGGGGACTA CATCGTCAAT GTGCAGCCCA TTGACGACGG CTGGATGTAC
    GGCACGGTGC AGAGAACTGG AAAAACCGGA ATGCTTCCAG CGAATTACAT TGAGTTTGTT
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92233
    Clone ID Related Accession (Same CDS sequence) XM_015473988.1
    Accession Version XM_015473988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulette isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGTTGG CCAGAAGGTG CACTGAACTC ATTAGTGATA TCCATTATAA AGAAGAGTAT 
    AAAAAGTCTA AAGGCAAGTG TATGTTTGTG ACTGACACGC CTATGCTAAA CCATGTAAAA
    CACATTGGTG CTTTTATTTC TGAGGCAAAA TACAAAGGCA CCATTAAGGC GGACCTTTCC
    AATTCTCTTT ATAAGCAGAT GCCAGCCACA ATTGACAGTG TCTTTGCCAG AGAAGTCACA
    CAACTACAGA GTGAGATTGC CTACAGGCAG AAGCATGATG CGGCCAAAGG AGTCTCAGAT
    TATGCCCACA TGAAGGAGCC GCCTGAGATC AAACATGCCA TGGAGGTCAA TAAGCACCAG
    AGTAATATTT TTTATAGAAA AGATGTACAG GACACCCACA TGTATAATGC AGAGCTTGAC
    AGACCAGACA TCAAGATGGC GACACAGATC TCCAAGATCA TAAGCGATGC TGAATACAAG
    AAAGGGCAAG GAGTAATGAA TAAAGAGCCT GCTGTAATTG GAAGACCAGA TTTTGAACAT
    GCTGTGGAAG CTTCTAAACT TTCTAGTCAA ATTAAATACA AAGAAAAATT TGATAGTGAA
    ATGAAGGATA AGAAACATCT TTACAATCCT CTTGAAAGTG CTTCTTTTAG GCAAAGTCAG
    CTGGCCACTG CCCTGGCGAG CAATGTGAAG TACAAGAAAG ACATTCAGAA TATGCATGAT
    CCAGTTTCAG GTCTCCCAAA CTTGTTCTTA GACCACGCTT TGAAAGTCAG CAAAATGCTA
    AGCGGACGAG AATATAGAAA GGTTTTTGAG GAAAACAAAG GCCTGTATCA TTTTGATGTG
    GACGCTGCGG AACACCTGCA CCATAAAGGC AATGCCATGC TCCAGAGTCA GGTGAAGTAC
    AAAGAAGAGT ATGAGAAAAA TAAGGGGAAA TCGATGCTTG AATTTGTTGA GACACCATCA
    TATCAAGTTT CAAAGGAGGC TCAGAAGATG CAGAGTGAGA AAGTTTACAG AGAGGATTTT
    GAGAAGGATA TTAAAGGAAG GTCATCAATG GATTTAGACA AGACTCCAGA GTTTTTACAT
    GTAAAGTACA TCACCAACCT TCTGAAGGAG AAAGAATATA AAAAGGATTT GGAAAATGAA
    ATAAAAGGGA AAGGAATGGA GCTTAATTCC GAAGTACTTG ATATTCAAAG AGCAAAACGA
    GCATCTGAAA TGACTAGTGA GAAAGAATAC AAGAAAGACC TGGAATCAGA AATTAAAGGG
    AAGGGAATGC AAGTTGGCAC TGACACCCTT GGAATTCAGC ATGCCAAAAG AGCTTCTGAG
    ATGGCGAGTG AGAAAGATTA TAAAAGAGAT CTGGAGACTG AAATTAGAGG GAAAGGCATG
    CAGGTGAGCA CAGACACTCT CGATATCCAG AGAGCCAAGA AAGCATCTGA AATGGCCAGC
    CAGAAAGAAT ATAAGAAAGA CTTAGAAAAT GAAATTAAAG GGAAAGGAAT GCAGGTGAGC
    ATGGATATCC CGGATATGCT TCGAGCCAAG AGGGCATCTG AAATCTATAG CCAGAAAAAG
    TATAAAGATG AGGCAGAGAA GATGCTTTCT AGCTATTCTA CTGTGGCAGA TACTCCTGAA
    ATTCAGAGAA TTAAGACAAC TCAACAAAAC ATTAGTGCGG TATTTTATAA GAAAGAAGTA
    GGAGCTGGCA CAGCAGTAAA AGATACTCCT GAGATTGAAC GAGTGAAGAA AAATCAGCAG
    AACATTAGTT CAGTAAAATA CAAAGAAGAG GTGAAACATG CAACAGCCAT TTCAGATCCA
    CCAGAACTCA AGAGAGTTAA AGAAAACCAG AAGAACATCA GCAATCTCAG GTACAAAGAG
    CCGTGTGACA GAGCAACTCC TGTGAGCATG ACGCCCGAGA TAGAGAGAGT GAGGAGAAAC
    CAGGAGCAGC TGAGCACGGT AAAATACAGA GGAGAGATTA AACAGGCGAC TGCAATTTCT
    GATCCACCAG AGCTGAAGAG AGTGAAAGAA AACCAGAAGA ACCTCAGCAA TGTTTATTAT
    AAATCTCAGA TGGGAAGAGC TACAGCTTTA AGTGTAACTC CTGAAATGGA AAGAGTAAAG
    AAGAATCAAG AAAATATTAG TTCGGTAAAA TATACCCAGG ACCAGAAACA GATGAAAGGT
    AGACCAAGTC TGATTTTAGA TACACCTGGT CTCAGACATG TCAAAGAAGC TCAAAATCGT
    ATTTCAATGG TGAAATATCA TGAAGATTTT GAAAAGACAA AGGGAAGGGG CTTTACTCCT
    GTTGTGGATG ACCCTGTGAC AGAGCGAGTG AGAAGGAACA CCCAGGTCGT CAGCGATGCT
    GCCTATAAAG GTGTCCATCC TCACATCGTG GAGATGGACA GGAGACCTGG AATCATTGTT
    GACCTCAAAG TTTGGCGCAC AGATCCTGGC TCCATCTTCG ACATTGATCC CCTGGAAGAC
    AATATTCAGT CTAGGAGTCT ACATATGCTT TCTGAAAAGG CGAATCTTTA TAGGAGACAA
    CGGTCTCGGT CTTACTCGAG CAGTACTTTT GGTACAGGTC TGGGAGATGA CAAATCAGAA
    ATATCTGAGA TTTACCGTAG CTTTTCATGC TGCAGTGAGG TAACAAGACC ATCTGATGAA
    GGAGCGCCTG TTCTTCCTGG AGCCTACCAG CAAAGCCATT CTCAAGGCTA TGGGTACATG
    CACCAGACCA GCCTGTCATC TGTGAGGTCA ATGCAGCATT CACCAAATCT AAGGACCTAC
    CGAGCCATGT ACGATTACAG TGCCCAGGAT GAAGATGAAG TCTCCTTCAG AGATGGGGAC
    TACATCGTCA ATGTGCAGCC CATTGACGAC GGCTGGATGT ACGGCACGGT GCAGAGAACT
    GGAAAAACCG GAATGCTTCC AGCGAATTAC ATTGAGTTTG TTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329474.1
    CDS621..3548
    Translation

    Target ORF information:

    RefSeq Version XM_015473988.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulette (NEBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGTTGG CCAGAAGGTG CACTGAACTC ATTAGTGATA TCCATTATAA AGAAGAGTAT 
    AAAAAGTCTA AAGGCAAGTG TATGTTTGTG ACTGACACGC CTATGCTAAA CCATGTAAAA
    CACATTGGTG CTTTTATTTC TGAGGCAAAA TACAAAGGCA CCATTAAGGC GGACCTTTCC
    AATTCTCTTT ATAAGCAGAT GCCAGCCACA ATTGACAGTG TCTTTGCCAG AGAAGTCACA
    CAACTACAGA GTGAGATTGC CTACAGGCAG AAGCATGATG CGGCCAAAGG AGTCTCAGAT
    TATGCCCACA TGAAGGAGCC GCCTGAGATC AAACATGCCA TGGAGGTCAA TAAGCACCAG
    AGTAATATTT TTTATAGAAA AGATGTACAG GACACCCACA TGTATAATGC AGAGCTTGAC
    AGACCAGACA TCAAGATGGC GACACAGATC TCCAAGATCA TAAGCGATGC TGAATACAAG
    AAAGGGCAAG GAGTAATGAA TAAAGAGCCT GCTGTAATTG GAAGACCAGA TTTTGAACAT
    GCTGTGGAAG CTTCTAAACT TTCTAGTCAA ATTAAATACA AAGAAAAATT TGATAGTGAA
    ATGAAGGATA AGAAACATCT TTACAATCCT CTTGAAAGTG CTTCTTTTAG GCAAAGTCAG
    CTGGCCACTG CCCTGGCGAG CAATGTGAAG TACAAGAAAG ACATTCAGAA TATGCATGAT
    CCAGTTTCAG GTCTCCCAAA CTTGTTCTTA GACCACGCTT TGAAAGTCAG CAAAATGCTA
    AGCGGACGAG AATATAGAAA GGTTTTTGAG GAAAACAAAG GCCTGTATCA TTTTGATGTG
    GACGCTGCGG AACACCTGCA CCATAAAGGC AATGCCATGC TCCAGAGTCA GGTGAAGTAC
    AAAGAAGAGT ATGAGAAAAA TAAGGGGAAA TCGATGCTTG AATTTGTTGA GACACCATCA
    TATCAAGTTT CAAAGGAGGC TCAGAAGATG CAGAGTGAGA AAGTTTACAG AGAGGATTTT
    GAGAAGGATA TTAAAGGAAG GTCATCAATG GATTTAGACA AGACTCCAGA GTTTTTACAT
    GTAAAGTACA TCACCAACCT TCTGAAGGAG AAAGAATATA AAAAGGATTT GGAAAATGAA
    ATAAAAGGGA AAGGAATGGA GCTTAATTCC GAAGTACTTG ATATTCAAAG AGCAAAACGA
    GCATCTGAAA TGACTAGTGA GAAAGAATAC AAGAAAGACC TGGAATCAGA AATTAAAGGG
    AAGGGAATGC AAGTTGGCAC TGACACCCTT GGAATTCAGC ATGCCAAAAG AGCTTCTGAG
    ATGGCGAGTG AGAAAGATTA TAAAAGAGAT CTGGAGACTG AAATTAGAGG GAAAGGCATG
    CAGGTGAGCA CAGACACTCT CGATATCCAG AGAGCCAAGA AAGCATCTGA AATGGCCAGC
    CAGAAAGAAT ATAAGAAAGA CTTAGAAAAT GAAATTAAAG GGAAAGGAAT GCAGGTGAGC
    ATGGATATCC CGGATATGCT TCGAGCCAAG AGGGCATCTG AAATCTATAG CCAGAAAAAG
    TATAAAGATG AGGCAGAGAA GATGCTTTCT AGCTATTCTA CTGTGGCAGA TACTCCTGAA
    ATTCAGAGAA TTAAGACAAC TCAACAAAAC ATTAGTGCGG TATTTTATAA GAAAGAAGTA
    GGAGCTGGCA CAGCAGTAAA AGATACTCCT GAGATTGAAC GAGTGAAGAA AAATCAGCAG
    AACATTAGTT CAGTAAAATA CAAAGAAGAG GTGAAACATG CAACAGCCAT TTCAGATCCA
    CCAGAACTCA AGAGAGTTAA AGAAAACCAG AAGAACATCA GCAATCTCAG GTACAAAGAG
    CCGTGTGACA GAGCAACTCC TGTGAGCATG ACGCCCGAGA TAGAGAGAGT GAGGAGAAAC
    CAGGAGCAGC TGAGCACGGT AAAATACAGA GGAGAGATTA AACAGGCGAC TGCAATTTCT
    GATCCACCAG AGCTGAAGAG AGTGAAAGAA AACCAGAAGA ACCTCAGCAA TGTTTATTAT
    AAATCTCAGA TGGGAAGAGC TACAGCTTTA AGTGTAACTC CTGAAATGGA AAGAGTAAAG
    AAGAATCAAG AAAATATTAG TTCGGTAAAA TATACCCAGG ACCAGAAACA GATGAAAGGT
    AGACCAAGTC TGATTTTAGA TACACCTGGT CTCAGACATG TCAAAGAAGC TCAAAATCGT
    ATTTCAATGG TGAAATATCA TGAAGATTTT GAAAAGACAA AGGGAAGGGG CTTTACTCCT
    GTTGTGGATG ACCCTGTGAC AGAGCGAGTG AGAAGGAACA CCCAGGTCGT CAGCGATGCT
    GCCTATAAAG GTGTCCATCC TCACATCGTG GAGATGGACA GGAGACCTGG AATCATTGTT
    GACCTCAAAG TTTGGCGCAC AGATCCTGGC TCCATCTTCG ACATTGATCC CCTGGAAGAC
    AATATTCAGT CTAGGAGTCT ACATATGCTT TCTGAAAAGG CGAATCTTTA TAGGAGACAA
    CGGTCTCGGT CTTACTCGAG CAGTACTTTT GGTACAGGTC TGGGAGATGA CAAATCAGAA
    ATATCTGAGA TTTACCGTAG CTTTTCATGC TGCAGTGAGG TAACAAGACC ATCTGATGAA
    GGAGCGCCTG TTCTTCCTGG AGCCTACCAG CAAAGCCATT CTCAAGGCTA TGGGTACATG
    CACCAGACCA GCCTGTCATC TGTGAGGTCA ATGCAGCATT CACCAAATCT AAGGACCTAC
    CGAGCCATGT ACGATTACAG TGCCCAGGAT GAAGATGAAG TCTCCTTCAG AGATGGGGAC
    TACATCGTCA ATGTGCAGCC CATTGACGAC GGCTGGATGT ACGGCACGGT GCAGAGAACT
    GGAAAAACCG GAATGCTTCC AGCGAATTAC ATTGAGTTTG TTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.