PIK3CB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIK3CB
Entrez Gene ID	517948
Full Name	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms	PI3K,PI3Kbeta
General protein information	Preferred Names phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta Names phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform PI3-kinase p110 subunit beta phosphoinositide-3-kinase, catalytic, beta polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024988896.1	XP_024844664.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1
NM_001206047.2	NP_001192976.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
XM_005201871.3	XP_005201928.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1
NM_001206047.1	NP_001192976.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIK3CB	NP_006210.1
	PIK3CB	XP_001113960.1
Xenopus tropicalis (tropical clawed frog)	pik3cb	NP_001072928.1
Danio rerio (zebrafish)	pik3cb	XP_005157523.1
Pan troglodytes (chimpanzee)	PIK3CB	XP_003950253.1
Canis lupus familiaris (dog)	PIK3CB	XP_534280.2
Bos taurus (cattle)	PIK3CB	NP_001192976.1
Mus musculus (house mouse)	Pik3cb	NP_083370.2
Rattus norvegicus (Norway rat)	Pik3cb	NP_445933.1
Gallus gallus (chicken)	PIK3CB	NP_001026482.1

Gene Ontology
Bibliography

Related articles in PubMed

20-HETE-induced nitric oxide production in pulmonary artery endothelial cells is mediated by NADPH oxidase, H2O2, and PI3-kinase/Akt.
Bodiga S, Gruenloh SK, Gao Y, Manthati VL, Dubasi N, Falck JR, Medhora M, Jacobs ER
American journal of physiology. Lung cellular and molecular physiology298(4)L564-74(2010 Apr)

PI3K, Rho, and ROCK play a key role in hypoxia-induced ATP release and ATP-stimulated angiogenic responses in pulmonary artery vasa vasorum endothelial cells.
Woodward HN, Anwar A, Riddle S, Taraseviciene-Stewart L, Fragoso M, Stenmark KR, Gerasimovskaya EV
American journal of physiology. Lung cellular and molecular physiology297(5)L954-64(2009 Nov)

Phosphoinositide 3-kinase Akt signaling pathway interacts with protein kinase Cbeta2 in the regulation of physiologic developmental hypertrophy and heart function.
Rigor DL, Bodyak N, Bae S, Choi JH, Zhang L, Ter-Ovanesyan D, He Z, McMullen JR, Shioi T, Izumo S, King GL, Kang PM
American journal of physiology. Heart and circulatory physiology296(3)H566-72(2009 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The current study was designed to determine mechanisms underlying 20-hydroxyeicosatetraenoic acid -stimulated nitric oxide (NO) release, and particularly the role of NADPH oxidase, reactive oxygen species, and PI3-kinase in stimulated NO release.
Title: 20-HETE-induced nitric oxide production in pulmonary artery endothelial cells is mediated by NADPH oxidase, H2O2, and PI3-kinase/Akt.

A functional contribution of PI3K, Rho, and ROCK to both the autocrine mechanism of ATP release and ATP-mediated angiogenic activation of vasa vasorum endothelial cells, is reported.
Title: PI3K, Rho, and ROCK play a key role in hypoxia-induced ATP release and ATP-stimulated angiogenic responses in pulmonary artery vasa vasorum endothelial cells.

Phosphoinositide 3-kinase Akt signaling pathway interacts with protein kinase Cbeta2 in the regulation of physiologic developmental hypertrophy and heart function.
Title: Phosphoinositide 3-kinase Akt signaling pathway interacts with protein kinase Cbeta2 in the regulation of physiologic developmental hypertrophy and heart function.

Introduction of a pH1 vector producing shRNA (short hairpin RNA) that targets p110beta abolished PIA [N6-(2-phenylisopropyl)adenosine]-induced Akt activation
Title: Specific role for p85/p110beta in GTP-binding-protein-mediated activation of Akt.

data demonstrate that hypoxia-induced adventitial fibroblast proliferation requires activation and interaction of PI3K, Akt, mTOR, p70S6K, and ERK1/2.
Title: Activation of phosphatidylinositol 3-kinase, Akt, and mammalian target of rapamycin is necessary for hypoxia-induced pulmonary artery adventitial fibroblast proliferation.

The following PIK3CB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3CB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36402	NM_001206047.2 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa15998	XM_005201871.3 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa36402	XM_024988896.1 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa36402	NM_001206047.1	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta (PIK3CB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIK3CB ( NM_001206047.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192976.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), mRNA.

Price & Availability↑

CloneID	OBa36402
Clone ID Related Accession (Same CDS sequence)	XM_024988896.1 , NM_001206047.2 , NM_001206047.1
Accession Version	NM_001206047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Nov 18, 2019 this sequence version replaced NM_001206047.1. ##Evidence-Data-START## Transcript exon combination :: GGVH01038207.1, SRR5571263.54148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192976.1
CDS	349..3561
Translation	MCFYFMMPPAMADVLDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISCIKQMLWKQVTNYPMFNLLMEIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGRGLHEFDALKDPEVNEFRIKMRRFSEEKIQSLVGLSWMDWLKHTYPPELEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKINELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHSLIQFQYIRNCVMNRTLPHFILVECCKIKKLYEQEMVAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEPLEFDINTCDLPRMTRLCFAVYAVLDKVKTKKSTKTMNPSKYQTIRKSGKVHYPVAWVNTMVFDFKGQLRSGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHIKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCRENFPHALPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLQQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALANRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKALSKQVEALNKLKTLNSLIKLNAMKLNRAKGKEAMHTCLKQNAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKTEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS

Target ORF information:

RefSeq Version	NM_001206047.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206047.2

ORF Insert Sequence:

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CB ( NM_001206047.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192976.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta (PIK3CB), mRNA.

Price & Availability↑

CloneID	OBa36402
Clone ID Related Accession (Same CDS sequence)	XM_024988896.1 , NM_001206047.2 , NM_001206047.1
Accession Version	NM_001206047.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03030405.1, AAFC03030408.1, AAFC03030407.1, AAFC03069410.1, AAFC03030404.1, AAFC03069409.1 and AAFC03117497.1. On Apr 20, 2011 this sequence version replaced XM_613754.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093728 [ECO:0000348] ##Evidence-Data-END##

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192976.1
CDS	322..3534
Translation	MCFYFMMPPAMADVLDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISCIKQMLWKQVTNYPMFNLLMEIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGRGLHEFDALKDPEVNEFRIKMRRFSEEKIQSLVGLSWMDWLKHTYPPELEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKINELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHSLIQFQYIRNCVMNRTLPHFILVECCKIKKLYEQEMVAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEPLEFDINTCDLPRMTRLCFAVYAVLDKVKTKKSTKTMNPSKYQTIRKSGKVHYPVAWVNTMVFDFKGQLRSGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHIKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCRENFPHALPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLQQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALANRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKALSKQVEALNKLKTLNSLIKLNAMKLNRAKGKEAMHTCLKQNAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKTEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS

Target ORF information:

RefSeq Version	NM_001206047.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta (PIK3CB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206047.1

ORF Insert Sequence:

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CB ( XM_005201871.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201928.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15998
Clone ID Related Accession (Same CDS sequence)	XM_005201871.3
Accession Version	XM_005201871.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103820.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC ATACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201928.1
CDS	227..3439
Translation	MCFYFMMPPAMADVLDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISCIKQMLWKQVTNYPMFNLLMEIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGRGLHEFDALKDPEVNEFRIKMRRFSEEKIQSLVGLSWMDWLKHTYPPELEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKINELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHSLIQFQYIRNCVMNRTLPHFILVECCKIKKLYEQEMVAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEPLEFDINTCDLPRMTRLCFAVYAVLDKVKTKKSTKTMNPSKYQTIRKSGKVHYPVAWVNTMVFDFKGQLRSGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHIKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCRENFPHALPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLQQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALANRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKALSKQVEALNKLKTLNSLIKLNAMKLNRAKGKEAMHTCLKQNAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKTEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS

Target ORF information:

RefSeq Version	XM_005201871.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201871.3

ORF Insert Sequence:

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC ATACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CB ( XM_024988896.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844664.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa36402
Clone ID Related Accession (Same CDS sequence)	XM_024988896.1 , NM_001206047.2 , NM_001206047.1
Accession Version	XM_024988896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844664.1
CDS	459..3671
Translation	MCFYFMMPPAMADVLDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISCIKQMLWKQVTNYPMFNLLMEIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGRGLHEFDALKDPEVNEFRIKMRRFSEEKIQSLVGLSWMDWLKHTYPPELEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKINELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHSLIQFQYIRNCVMNRTLPHFILVECCKIKKLYEQEMVAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEPLEFDINTCDLPRMTRLCFAVYAVLDKVKTKKSTKTMNPSKYQTIRKSGKVHYPVAWVNTMVFDFKGQLRSGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHIKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCRENFPHALPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLQQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALANRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKALSKQVEALNKLKTLNSLIKLNAMKLNRAKGKEAMHTCLKQNAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKTEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS

Target ORF information:

RefSeq Version	XM_024988896.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988896.1

ORF Insert Sequence:

ATGTGCTTCT ACTTCATGAT GCCTCCTGCT ATGGCAGACG TCCTTGACAT CTGGGCAGTG 
GACTCACAGA TAGCGTCTGA TGGCTCCATA CCCGTGGATT TTCTTTTGCC CACTGGGATT 
TATATCCAGT TGGAGGTGCC TCGTGAAGCT ACCATTTCCT GTATTAAGCA GATGTTATGG 
AAACAAGTTA CCAATTACCC AATGTTTAAC CTCCTTATGG AAATCGACTC CTATATGTTT 
GCATGTGTGA ATCAAACTGC TGTATATGAG GAACTCGAAG ATGAAACAAG AAGACTCTGT 
GATGTCAGAC CTTTTCTTCC AGTTCTCAAA TTAGTGACAA GAAGTTGTGA CCCAGGGGAA 
AAATTAGACT CAAAAATTGG AGTCCTTATA GGAAGAGGTC TGCATGAATT TGATGCCTTG 
AAGGATCCTG AAGTGAATGA ATTCCGAATA AAAATGCGCA GATTCAGTGA GGAAAAGATT 
CAGTCGCTTG TGGGATTATC TTGGATGGAT TGGCTAAAGC ATACCTATCC ACCTGAGCTT 
GAACCATCCA TCCCTGAAAA TTTAGAAGAT AAACTTTATG GAGGAAAGCT CATTGTGGCT 
GTTCATTTTG AAAATTGTCA GGATGTGTTT AGTTTTCAGG TATCTCCTAA TATGAATCCT 
ATCAAAATAA ATGAATTGGC AATCCAAAAA CGTCTGACTA TTCATGGGAA AGAAGATGAA 
GTTAGCCCTT ATGACTATGT GTTGCAAGTC AGTGGGAGAG TAGAATATGT GTTTGGTGAT 
CACTCACTAA TTCAGTTCCA GTATATCCGG AACTGTGTAA TGAACAGAAC ATTGCCCCAT 
TTTATACTTG TGGAATGTTG CAAGATCAAG AAGCTGTATG AACAGGAGAT GGTTGCCATA 
GAGGCTGCCA TAAATCGAAA TTCATCTAAC CTTCCTCTTC CTTTACCACC AAAGAAAACT 
CGAATTATTT CTCATGTTTG GGAAAATAAC AATCCTTTCC AAATTGTCTT GGTTAAAGGA 
AATAAACTTA ACACAGAAGA AACTGTAAAA GTTCATGTCA GGGCCGGCCT TTTTCATGGT 
ACCGAACTCC TGTGTAAAAC TGTCGTAAGC TCAGAGATAT CAGGGAAGAA TGATCATATT 
TGGAATGAAC CACTGGAATT TGATATCAAC ACTTGTGACC TGCCACGAAT GACTCGATTA 
TGTTTTGCTG TTTATGCAGT TTTGGATAAA GTGAAAACTA AGAAATCAAC TAAAACCATG 
AATCCCTCTA AGTATCAGAC CATCAGGAAA TCTGGAAAAG TGCATTATCC TGTAGCCTGG 
GTAAATACGA TGGTTTTTGA CTTTAAAGGA CAGTTGAGAT CTGGAGATAT AATATTACAC 
AGCTGGTCTT CATTTCCTGA TGAACTTGAA GAAATGTTGA ATCCAATGGG AACTGTTCAA 
ACAAATCCAT ATACAGAAAA TGCAACAGCT TTGCACATAA AATTCCCAGA GAATAAAAAA 
CAACCTTATT ACTATCCTCC CTTCGATAAG ATTATTGAAA AAGCAGCTGA GATTGCAAGC 
AGTGATAGTG CTAATGTATC AAGTCGAGGT GGAAAGAAGT TTCTTGCTGT ACTGAAAGAA 
ATCTTGGACA GGGATCCTTT GTCTCAACTG TGTGAGAATG AAATGGATCT TATTTGGACT 
TTGCGACAAG ACTGCAGAGA GAATTTCCCA CACGCGTTGC CGAAATTGCT GCTGTCGATC 
AAGTGGAATA AACTTGAGGA TGTTGCTCAG CTTCAAGCAC TGCTTCAGAT TTGGCCTAAA 
CTGCCCCCCC GGGAGGCCCT TGAGCTTCTA GATTTCAACT ATCCAGATCA GTATGTGCGA 
GAATATGCTG TGGGCTGCCT GCAACAGATG AGTGATGAAG AACTCTCTCA ATATCTTTTA 
CAGTTGGTGC AAGTTTTAAA ATATGAGCCT TTTCTTGATT GTGCCTTGTC TAGATTCCTA 
CTAGAAAGAG CACTTGCTAA TCGGAGGATA GGGCAGTTTT TATTTTGGCA CCTCAGATCT 
GAAGTACACA TTCCTGCTGT TTCAGTACAA TTTGGTGTTA TCCTTGAAGC GTACTGCCGG 
GGAAGCGTAG GTCACATGAA GGCGCTTTCT AAGCAGGTTG AAGCACTCAA TAAACTAAAA 
ACTTTAAATA GTTTAATCAA ACTGAATGCA ATGAAGCTAA ATAGAGCCAA AGGGAAAGAA 
GCAATGCACA CTTGTTTAAA ACAGAATGCT TACCGTGAAG CCCTCTCTGA CCTGCAGTCA 
CCTCTCAACC CATGTGTTAT CCTCTCAGAA CTCTATGTTG AGAAGTGTAA ATACATGGAT 
TCCAAAATGA AGCCTTTGTG GCTTGTGTAC AACAACAAGG TGTTTGGTGA GGATTCAGTT 
GGAGTGATTT TTAAAAATGG TGATGATTTA CGGCAGGATA TGTTGACACT CCAAATGCTG 
CGCTTGATGG ATTTACTCTG GAAAGAAGCT GGTCTGGACC TTCGGATGCT ACCTTACGGC 
TGCTTAGCAA CAGGAGACCG CTCTGGCCTC ATTGAAGTTG TGAGCACCTC TGAAACAATT 
GCTGACATTC AGCTGAACAG TAGCAATGTT GCTGCAGCAG CAGCCTTCAA CAAAGATGCC 
CTTCTGAACT GGCTTAAAGA ATACAATTCT GGGGATGACC TGGACCGAGC CATTGAGGAG 
TTTACCCTAT CCTGTGCCGG CTACTGTGTA GCTTCTTATG TCCTTGGGAT TGGTGACAGA 
CATAGTGATA ACATCATGGT CAAAAAAACT GGCCAGCTCT TCCACATTGA CTTTGGACAT 
ATTCTTGGAA ATTTCAAGTC TAAATTTGGC ATTAAAAGGG AGCGAGTGCC TTTTATTCTT 
ACTTATGATT TCATTCATGT GATTCAACAA GGAAAAACAG GAAACACAGA AAAATTTGGC 
CGGTTCCGCC AGTGTTGTGA GGATGCATAT CTGATTTTAC GCCGGCATGG GAATCTCTTC 
ATCACGCTCT TTGCACTGAT GTTGACTGCA GGGCTTCCTG AGCTCACCTC AGTCAAGGAT 
ATACAGTATC TTAAGGACTC TCTTGCCTTA GGGAAGACTG AAGAAGAAGC ACTCAAGCAA 
TTCAAGCAAA AATTTGATGA GGCACTCAGG GAAAGTTGGA CTACTAAAGT CAATTGGATG 
GCCCACACAG TTCGCAAAGA CTACAGATCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

20-HETE-induced nitric oxide production in pulmonary artery endothelial cells is mediated by NADPH oxidase, H2O2, and PI3-kinase/Akt.
American journal of physiology. Lung cellular and molecular physiology298(4)L564-74(2010 Apr)
Bodiga S,Gruenloh SK,Gao Y,Manthati VL,Dubasi N,Falck JR,Medhora M,Jacobs ER

PI3K, Rho, and ROCK play a key role in hypoxia-induced ATP release and ATP-stimulated angiogenic responses in pulmonary artery vasa vasorum endothelial cells.
American journal of physiology. Lung cellular and molecular physiology297(5)L954-64(2009 Nov)
Woodward HN,Anwar A,Riddle S,Taraseviciene-Stewart L,Fragoso M,Stenmark KR,Gerasimovskaya EV

Phosphoinositide 3-kinase Akt signaling pathway interacts with protein kinase Cbeta2 in the regulation of physiologic developmental hypertrophy and heart function.
American journal of physiology. Heart and circulatory physiology296(3)H566-72(2009 Mar)
Rigor DL,Bodyak N,Bae S,Choi JH,Zhang L,Ter-Ovanesyan D,He Z,McMullen JR,Shioi T,Izumo S,King GL,Kang PM

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PIK3CB cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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