RHBDF2 cDNA ORF clone, Bos taurus(cattle)

The following RHBDF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHBDF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10433 XM_010816618.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_010824327.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_010816616.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_010816617.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_010824326.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_005195850.3
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_005221303.3
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10433 XM_010824325.2
Latest version!
Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa227025 XM_010816617.3
Latest version!
Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227025 XM_005221303.4
Latest version!
Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227025 XM_010816618.3
Latest version!
Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227025 XM_010816616.3
Latest version!
Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227026 XM_024981047.1
Latest version!
Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010816618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814920.1
    CDS317..2809
    Translation

    Target ORF information:

    RefSeq Version XM_010816618.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_005221303.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221303.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221360.1
    CDS248..2740
    Translation

    Target ORF information:

    RefSeq Version XM_005221303.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221303.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010816616.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814918.1
    CDS331..2823
    Translation

    Target ORF information:

    RefSeq Version XM_010816616.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816616.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010824326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822628.1
    CDS1932..4424
    Translation

    Target ORF information:

    RefSeq Version XM_010824326.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_005195850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195907.1
    CDS248..2740
    Translation

    Target ORF information:

    RefSeq Version XM_005195850.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010816617.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814919.1
    CDS1932..4424
    Translation

    Target ORF information:

    RefSeq Version XM_010816617.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010824327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822629.1
    CDS317..2809
    Translation

    Target ORF information:

    RefSeq Version XM_010824327.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10433
    Clone ID Related Accession (Same CDS sequence) XM_010816618.2 , XM_005221303.3 , XM_010816616.2 , XM_010824326.2 , XM_005195850.3 , XM_010816617.2 , XM_010824327.2 , XM_010824325.2
    Accession Version XM_010824325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822627.1
    CDS331..2823
    Translation

    Target ORF information:

    RefSeq Version XM_010824325.2
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTCTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227025
    Clone ID Related Accession (Same CDS sequence) XM_005221303.4 , XM_010816616.3 , XM_010816618.3 , XM_010816617.3
    Accession Version XM_005221303.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221303.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221360.1
    CDS263..2755
    Translation

    Target ORF information:

    RefSeq Version XM_005221303.4
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221303.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227025
    Clone ID Related Accession (Same CDS sequence) XM_005221303.4 , XM_010816616.3 , XM_010816618.3 , XM_010816617.3
    Accession Version XM_010816616.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814918.1
    CDS339..2831
    Translation

    Target ORF information:

    RefSeq Version XM_010816616.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816616.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227025
    Clone ID Related Accession (Same CDS sequence) XM_005221303.4 , XM_010816616.3 , XM_010816618.3 , XM_010816617.3
    Accession Version XM_010816618.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814920.1
    CDS378..2870
    Translation

    Target ORF information:

    RefSeq Version XM_010816618.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816618.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227025
    Clone ID Related Accession (Same CDS sequence) XM_005221303.4 , XM_010816616.3 , XM_010816618.3 , XM_010816617.3
    Accession Version XM_010816617.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814919.1
    CDS414..2906
    Translation

    Target ORF information:

    RefSeq Version XM_010816617.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816617.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AGTAAGGAAG AAATGAGCTC CATGCCCGAT
    GACGTGTTTG AGTCGCCCCC ACTCTCCGCC AGCTACTTCC GGGGGATCCC ACACTCAGCC
    TCCCCAGTCT CTCCCGACCA GATTCCCCTG AAGGAGTCCA GCCGAGCCCC AGTGCCCCCC
    ACCAAACGCG GCAAGCGCAT TGCCTCCAAG GTGAAGCACT TTGCCTTCGA CCGGAAGAAG
    CGGCACTACG GCCTGGGTGT GGTGGGCAAC TGGCTGAACC GCACTTACCG CCGCAGCATC
    AGCAGCACGG TGCAGCGGCA GCTGGAGAGC TTCGACAGCC ACCGGCCCTA CTTCACCTAC
    TGGCTAACCT TTGTCCACGT CATCATCACA TTGCTGGTGA TCGGCACGTA CGGCATCGCG
    CCCGTGGGCT TCGCCCAGCA CATCACCACC CAGCTGGTGC TCAGGAACAA AGGTGTGTAT
    GAGAGCGTGA AGTACATCCA GCAGGAGAAC TTTTGGATTG GCCCCAGCTC GATCGACTTG
    ATCCACCTGG GAGCCAAGTT CTCACCTTGC ATCCGGAAGG ACCAACAGAT CGAGCAGCTC
    GTGCTGCGGG AGCGAGACCT GGAGCGGGAC TCGGGCTGCT GTGTCCAAAA CGACCGTTCA
    GGCTGCATCC AGACACAGAG GAAGGACTGC TCGGAGACTC TGGCCACTTT CGTCAAGTGG
    CAGGATGATA CAGGGCCCCC CATGGACAAG TCTGATCTGG GCCAGAAGCG GACTTCTGGG
    GCCGTGTGCA ACCAAGACCC CAGAACCTGT GAGGAGCCGG CCTCTAGTGG TGCCCACATC
    TGGCCCGACG ACATCACCAA GTGGCCGATC TGCACGGAGC AGGCCAAGAG TAACCGCTCG
    GGCTTCCCGC ACATGGACTG CCAGATCCGG GGCCGTCCCT GCTGCATCGG CACCAAGGGC
    AGCTGTGAGA TCACCACGCG CGAATACTGT GAGTTCATGC ACGGCTATTT CCACGAGAAG
    GCCACACTCT GCTCCCAGGT GCACTGCTTG GACAAGGTGT GTGGACTGCT GCCCTTCCTC
    AACCCCGAGG TCCCCGATCA GTTCTATAGG CTCTGGCTGT CTCTGTTCCT CCACGCTGGC
    GTCATCCACT GCCTCGTGTC TGTGATCTTC CAAATGACCA TCCTGCGTGA CCTGGAGAAG
    CTGGCTGGCT GGCACCGCAT CTCCATTATC TTCATTCTCA GCGGCATCAC TGGCAACCTT
    GCCAGCGCCC TCTTCCTTCC ATACCGGGCA GAGGTGGGCC CTGCAGGGTC GCAGTTCGGC
    CTCCTGGCCT GCCTCTTCGT GGAGCTCTTC CAGAGCTGGC AGCTGCTGGA GCGGCCCTGG
    AAGGCCTTCC TGAACCTGTC GGCCATCGTG CTGTTCCTCT TCATCTGCGG CCTCCTGCCC
    TGGATCGACA ACATCGCCCA CATCTTCGGC TTCCTCAGCG GGCTGCTGCT GGCCTTCGCC
    TTCCTGCCCT ACATCACCTT CGGCACGAGC GACAAGTACC GCAAGCGCGC CCTCATCCTG
    GTGTCGCTGG TGGTCTTCGC CGGCCTTTTT GCCTCGCTGG TCATCTGGCT GTACATCTAC
    CCCATCCACT GGCCCTGGGT CGAGTACCTC ACCTGCTTCC CCTTCACCAG CCGCTTCTGC
    GAGAAGTATG AGTTGGACCA GGTGCTACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227026
    Clone ID Related Accession (Same CDS sequence) XM_024981047.1
    Accession Version XM_024981047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive rhomboid protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AAAATGAGCT CCATGCCCGA TGACGTGTTT
    GAGTCGCCCC CACTCTCCGC CAGCTACTTC CGGGGGATCC CACACTCAGC CTCCCCAGTC
    TCTCCCGACC AGATTCCCCT GAAGGAGTCC AGCCGAGCCC CAGTGCCCCC CACCAAACGC
    GGCAAGCGCA TTGCCTCCAA GGTGAAGCAC TTTGCCTTCG ACCGGAAGAA GCGGCACTAC
    GGCCTGGGTG TGGTGGGCAA CTGGCTGAAC CGCACTTACC GCCGCAGCAT CAGCAGCACG
    GTGCAGCGGC AGCTGGAGAG CTTCGACAGC CACCGGCCCT ACTTCACCTA CTGGCTAACC
    TTTGTCCACG TCATCATCAC ATTGCTGGTG ATCGGCACGT ACGGCATCGC GCCCGTGGGC
    TTCGCCCAGC ACATCACCAC CCAGCTGGTG CTCAGGAACA AAGGTGTGTA TGAGAGCGTG
    AAGTACATCC AGCAGGAGAA CTTTTGGATT GGCCCCAGCT CGATCGACTT GATCCACCTG
    GGAGCCAAGT TCTCACCTTG CATCCGGAAG GACCAACAGA TCGAGCAGCT CGTGCTGCGG
    GAGCGAGACC TGGAGCGGGA CTCGGGCTGC TGTGTCCAAA ACGACCGTTC AGGCTGCATC
    CAGACACAGA GGAAGGACTG CTCGGAGACT CTGGCCACTT TCGTCAAGTG GCAGGATGAT
    ACAGGGCCCC CCATGGACAA GTCTGATCTG GGCCAGAAGC GGACTTCTGG GGCCGTGTGC
    AACCAAGACC CCAGAACCTG TGAGGAGCCG GCCTCTAGTG GTGCCCACAT CTGGCCCGAC
    GACATCACCA AGTGGCCGAT CTGCACGGAG CAGGCCAAGA GTAACCGCTC GGGCTTCCCG
    CACATGGACT GCCAGATCCG GGGCCGTCCC TGCTGCATCG GCACCAAGGG CAGCTGTGAG
    ATCACCACGC GCGAATACTG TGAGTTCATG CACGGCTATT TCCACGAGAA GGCCACACTC
    TGCTCCCAGG TGCACTGCTT GGACAAGGTG TGTGGACTGC TGCCCTTCCT CAACCCCGAG
    GTCCCCGATC AGTTCTATAG GCTCTGGCTG TCTCTGTTCC TCCACGCTGG CGTCATCCAC
    TGCCTCGTGT CTGTGATCTT CCAAATGACC ATCCTGCGTG ACCTGGAGAA GCTGGCTGGC
    TGGCACCGCA TCTCCATTAT CTTCATTCTC AGCGGCATCA CTGGCAACCT TGCCAGCGCC
    CTCTTCCTTC CATACCGGGC AGAGGTGGGC CCTGCAGGGT CGCAGTTCGG CCTCCTGGCC
    TGCCTCTTCG TGGAGCTCTT CCAGAGCTGG CAGCTGCTGG AGCGGCCCTG GAAGGCCTTC
    CTGAACCTGT CGGCCATCGT GCTGTTCCTC TTCATCTGCG GCCTCCTGCC CTGGATCGAC
    AACATCGCCC ACATCTTCGG CTTCCTCAGC GGGCTGCTGC TGGCCTTCGC CTTCCTGCCC
    TACATCACCT TCGGCACGAG CGACAAGTAC CGCAAGCGCG CCCTCATCCT GGTGTCGCTG
    GTGGTCTTCG CCGGCCTTTT TGCCTCGCTG GTCATCTGGC TGTACATCTA CCCCATCCAC
    TGGCCCTGGG TCGAGTACCT CACCTGCTTC CCCTTCACCA GCCGCTTCTG CGAGAAGTAT
    GAGTTGGACC AGGTGCTACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836815.1
    CDS339..2822
    Translation

    Target ORF information:

    RefSeq Version XM_024981047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus rhomboid 5 homolog 2 (RHBDF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTCCG ATGACAAGAA CGGAGAGAGG GTCTCCTCGG TGTCCAGCAG CCGCCTACAG 
    AGCCGGAAGC CGCCCAACCT GTCCATCACC ATCCCTCCGC CCGAGACCTC GGCCCCCAGC
    GAGCAGGCCA GCATGCTGCC CCAGGAATCC GGAGGGCAGA GGCACAGGAA CCCGGCCCTC
    CTGAAGAGCG TCAGCCTCCA GGAGCCACGA GGGCGATGGC AGGAGGGCAG CCCAGAGAAG
    CGTCCTGGCT TCCGCCGCCA GGCCTCGCTG TCCCAGAGCA TCCGCAAGGG CGCGGCCCAG
    TGGTTCGGGG TCAGTGGAGA CTGGGAACTG AAGCGGCAGC ACTGGCAGCG CCGGAGCCTT
    CACCACTGCA GCGTGCGCTA CGGCCGGCTC AAGGCCTCGT GCCAGCGGGA CCTGGAGCTC
    CCCAGCCAAG AGGTGCCCTC CTTCCAGGAC ACTGAGTCTC CAAAGCCCTG CAAGATGCCC
    AAGATTGTGG ACCCCCTGGC CCGAGGACGG GCATTCCGCC ACCCGGATGA GGTGGACCGG
    CCCCACGCCC CGCACCCACC GCTGACCCCC GGGGTCCTTT CCCTCACCTC CTTCACCAGC
    GTCCGCTCCG GCTATTCCCA CCTGCCCCGC CGCAAGAGGA TCTCTGTGGC CCACATGAGC
    TTTCAAGCTG CTGCAGCCTT GCTTAAGGGG CGCTCAGTGC TAGAGGCCAC AGGACAGCGG
    AGCCGGGTGG TCAAGTGCAG CTTTGCCTAC CCCAGCTCCC TGGAGGAGGA CGTGGTCGAC
    GGGGCGGAGA CATTCGACTC CTCGTTTTTT AAAATGAGCT CCATGCCCGA TGACGTGTTT
    GAGTCGCCCC CACTCTCCGC CAGCTACTTC CGGGGGATCC CACACTCAGC CTCCCCAGTC
    TCTCCCGACC AGATTCCCCT GAAGGAGTCC AGCCGAGCCC CAGTGCCCCC CACCAAACGC
    GGCAAGCGCA TTGCCTCCAA GGTGAAGCAC TTTGCCTTCG ACCGGAAGAA GCGGCACTAC
    GGCCTGGGTG TGGTGGGCAA CTGGCTGAAC CGCACTTACC GCCGCAGCAT CAGCAGCACG
    GTGCAGCGGC AGCTGGAGAG CTTCGACAGC CACCGGCCCT ACTTCACCTA CTGGCTAACC
    TTTGTCCACG TCATCATCAC ATTGCTGGTG ATCGGCACGT ACGGCATCGC GCCCGTGGGC
    TTCGCCCAGC ACATCACCAC CCAGCTGGTG CTCAGGAACA AAGGTGTGTA TGAGAGCGTG
    AAGTACATCC AGCAGGAGAA CTTTTGGATT GGCCCCAGCT CGATCGACTT GATCCACCTG
    GGAGCCAAGT TCTCACCTTG CATCCGGAAG GACCAACAGA TCGAGCAGCT CGTGCTGCGG
    GAGCGAGACC TGGAGCGGGA CTCGGGCTGC TGTGTCCAAA ACGACCGTTC AGGCTGCATC
    CAGACACAGA GGAAGGACTG CTCGGAGACT CTGGCCACTT TCGTCAAGTG GCAGGATGAT
    ACAGGGCCCC CCATGGACAA GTCTGATCTG GGCCAGAAGC GGACTTCTGG GGCCGTGTGC
    AACCAAGACC CCAGAACCTG TGAGGAGCCG GCCTCTAGTG GTGCCCACAT CTGGCCCGAC
    GACATCACCA AGTGGCCGAT CTGCACGGAG CAGGCCAAGA GTAACCGCTC GGGCTTCCCG
    CACATGGACT GCCAGATCCG GGGCCGTCCC TGCTGCATCG GCACCAAGGG CAGCTGTGAG
    ATCACCACGC GCGAATACTG TGAGTTCATG CACGGCTATT TCCACGAGAA GGCCACACTC
    TGCTCCCAGG TGCACTGCTT GGACAAGGTG TGTGGACTGC TGCCCTTCCT CAACCCCGAG
    GTCCCCGATC AGTTCTATAG GCTCTGGCTG TCTCTGTTCC TCCACGCTGG CGTCATCCAC
    TGCCTCGTGT CTGTGATCTT CCAAATGACC ATCCTGCGTG ACCTGGAGAA GCTGGCTGGC
    TGGCACCGCA TCTCCATTAT CTTCATTCTC AGCGGCATCA CTGGCAACCT TGCCAGCGCC
    CTCTTCCTTC CATACCGGGC AGAGGTGGGC CCTGCAGGGT CGCAGTTCGG CCTCCTGGCC
    TGCCTCTTCG TGGAGCTCTT CCAGAGCTGG CAGCTGCTGG AGCGGCCCTG GAAGGCCTTC
    CTGAACCTGT CGGCCATCGT GCTGTTCCTC TTCATCTGCG GCCTCCTGCC CTGGATCGAC
    AACATCGCCC ACATCTTCGG CTTCCTCAGC GGGCTGCTGC TGGCCTTCGC CTTCCTGCCC
    TACATCACCT TCGGCACGAG CGACAAGTAC CGCAAGCGCG CCCTCATCCT GGTGTCGCTG
    GTGGTCTTCG CCGGCCTTTT TGCCTCGCTG GTCATCTGGC TGTACATCTA CCCCATCCAC
    TGGCCCTGGG TCGAGTACCT CACCTGCTTC CCCTTCACCA GCCGCTTCTG CGAGAAGTAT
    GAGTTGGACC AGGTGCTACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.