NHSL1 cDNA ORF clone, Bos taurus(cattle)

The following NHSL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237755 XM_024996598.1
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa39149 NM_001191538.1
Latest version!
Bos taurus NHS like 1 (NHSL1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa22716 XM_005210988.4
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa22718 XM_005210990.4
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa22719 XM_024996597.1
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91507 XM_015472884.1
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91508 XM_015472885.1
Latest version!
Bos taurus NHS like 1 (NHSL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OBa22718 XM_005210990.3 Bos taurus NHS like 1 (NHSL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa22716 XM_005210988.3 Bos taurus NHS like 1 (NHSL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237755
    Clone ID Related Accession (Same CDS sequence) XM_024996598.1
    Accession Version XM_024996598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGACAGC GCTCAGAAAC CAGGGATTCC AGCTGTCAGA CTGAGGAAGT GAAGGTCGTA 
    CCACCTTCAA TGAGAAGAAT CAGAGCCCAG AAGGGGCAAG GCATTGCTGC TCAGATGAGT
    CACTTCTCAG GCTCCTCTGG GAACATGTCA GTGCTGAGTG ACTCTGCCGG GGTCGTGTTC
    CCTTCCCGCC TCAACAGCGA TGCTGGTTTT CACAGTCTTC CCCGGTCTGG AGCAAGGGCA
    AATGTTCAGG CCCTGGAGCC ACGGCTGGGC GCCCTGGGCC CTGCGGAAGA CCTGGGTGGC
    GCTTTCCCCT ACCAGCAGGG TCACCCACAA GTAGATGAAG ACTTTGGGCA TCTAGGAGCT
    GCCCCGAGGA TGGGAACACT GTTGAGGCCC AAATCTCAGG AGCTGAGGCA CTTCGAGGGT
    GAAAATGTAA CCAGCCCTGC GTGTGTGGTG TCTCCTCACA CGAGCTACTC CACCAGCATC
    ATCCCAAATG CCACGCTCTC CTCCTCTTCA GAGATCATTG TGATTCACAC TGCTCAGAGC
    TCAGGGCAGC TGGACAGTAA AATTACCAGC TCATCTTCAT ACTCAAAGGT CAGATCCAGA
    GACCACCTCC TCTCCAGGCA GAAAGATGGT CATCATTCGT CCAGTGCAAC CACACTGCTG
    TCCCTCTGTG ACTCCGAAGT CTCTCTAAAT ACACCAGCAA ATCGGGAGAA TGGGTCCCAG
    GCCATGGCCT ATAACTGTAG AAATAACCTT AGCATCCCAG CCCACCCCCA GGATGTGGAC
    AACAAGAGTG AGTCCAGTTA TTCAGGCAGC CGGGGCCAGG GTGGTAGCGT GGAGCCCTGG
    GAATATGGTG GCTCAGGTAG TGGGCGGGCA TCCCCACTGA AGCCACATTT GGCAGCTCCT
    GGTTACTCCA CTCCTGTAAG CAACACGAGC AGCTGCAGTT TGGACCAGAC CTCTAACAAG
    GATGATACCA GGTCTGTGTA TTCAGAGGAC CACGATGGCT ACTCCCCTAC TCTGCACCCG
    GACTCTGGAC ACAGACCTGG GGATCTGTGC AATAGTAATG ATGGCTTTGG GAACCCCAGG
    CACAGCGTGG TCAATGTTTT TGATGGAAGA GCTCAGAGAC ACCAAGGGGA CCGGTCCAAT
    TGCCAAGACA AATCCCTCGC GCGAAGCATT TCTTTGAAGA AAGCCAAGAA GCCTCCCCTG
    CCACCCTCTC GGACGGACTC TCTGCGCAGG GTTCCCAAGA AGAACGTCCA GTCGAACGGG
    CAGGTGCTGA ACGAGAGCCT GATCGCCTCT CTCCAGCACT CGCTGCAGCT GAACCTCCCG
    GGCAAGGGCG GCAGCTCGCC CTCCCAGAGC CCCTGCAGTG ATTTCGAAGA ACCGTGGCTG
    CCTCGCTCCC GCAGCCAGAG CACTGTGAGC GCCGGCAGCA GCCTAACCTC CGCCACGGCC
    CCCAACGTCT ACTCCGTGTG CGGGCTCACC CCGGCACAGA GCGACACGAG CAGCGTCAAG
    TCCGAGTACA CAGACCCGTG GGGTTACTAC ATTGACTACA CGGGAGTACA GGAAGATCAG
    GGGAACGCAG GCAGGGCCGG TCCGGCCGGC AGCGCAGCAT CACCTGGAAG TGGGCCGGGC
    CGCCATCTCC TGGAGGGGTC CCGGGCTGCA GTACCCCCAG TGCCCGGTGG CGCCGTCAAA
    CCAAAGATCA CATCACCGGA GAAGTCGCAC AGAGTCACTT CTCCATCCAG TGGGTATTCC
    AGTCAGTCGA ACACGCCCAC CGCTCTCACC CCTGTGCCTG TGTTCTTAAA ATCAATGTCA
    CCAGCCAATG GGAAGGGAAA GCCCAAACCC AAGGTGCCAG AAAGGAAGTC GTCTCTTGTA
    TCTTCAGTAT CCGTCTCCTC CTCCTCCACG TCTCTTTCCT CGAATACTTC CACTGAAGGA
    AGCGGGACGG TGAAGAAGCT GGATTCAGCG CTGGTGTCTC CGCTCGCGGC TGCTCCTCAC
    ACCTCTCCTC CAGCCCCTTG TCCCGCTGAC AAGTCTCCTT TCCTCCCTCC CCCGCCACCT
    CTGGCAGATT TCTCCGAGGG CTCACCTCTG CCTCGCTCTC CCCTCTTCCC CCCTCCGCCA
    CCAGAAGTGC TCACTCCCTT CTGTGCCCCC ACCGACGCCT GCTTTCCTCC GCCCCCTACA
    GCACTGAGCG CCCCTTTTCT GGATTCATCT GACTCCCTGC CACCTCCGCC ACCTGCCTTA
    ACCCCTTCAG TGCCCCCGCC TGCCCCACCG CTTGACCCCA AATTGATGAA AGATGCCAGG
    CCTTCTTTCA AGAAACCTGG CCAGCCAGAG CCCTCCCGGG AGGCCTGCAG ACAGCCTTCC
    ATCAAGGAGG AGGGCAGCAG ACTCCCCATG CCCCTGATCA CCACCGAAGC GTTGCAAATG
    GTGCAGTTGA GGCCGGTGAA GAAGAACTCA GGAACCGAGG TAGCCCCCTT ACAGGAACAC
    GCATCTCAGG AAAAACGAAC TCCGATTGTT TCCCAGTATC ATTTAAAGCC ATCTGCTTTC
    CTGAAATCCC GAAATAGCAT ACATGAAATG GAGAGTGAAA GCCAGCCTGC CTCCGCGACG
    AGCTCGCTCC CGACTCCTGC CAAGAGCTCG ACTCAGGGTC ACCAGGACAG TGCAGCCGAG
    CATGGCCTCC AGAGCCACAG CCCGGCCGGG GAGGCGGAGG CCGGAGCGGG TGCCACCCCG
    CCCCTCGGCC CGTCGCCCAG CAGGAAGCCG CCCCCCATTT CCAAGAAGCC CAAACTGTTC
    CTCGTGGTGT CACCTCCGCA GAGGGATTTC ACGGCGGAGC CCGCAGAGAA CGTGAGCGTG
    GCGTCTCCCA GCCCCACGAG CGGAGAGGCA CGAGAGAGGG GTGCAGCAGG GGCTGGTTCC
    GATGACACCG ACTCTGGCAG CTCGGTGGTT CTTGTGGGAG GAGCTGCAGG ATCCGAGTCC
    CCGGGCAGAG TGGAAGCCAA TGTCCCCATG GTGCCAGCCC CTGCGGAGGA GCCGGGTGCC
    AGGAACGGTG TGGAGCGCTG CCTGCCTCCG CAGGACCCAG GGCCTGGGGT GCCGGAGGCT
    GAGACAGCCA CTTCTTCTTC AGAGACCTGT GACTTCCCTA AGGACGACGG GGGTGATGAG
    GTGATGACCC CCAGTAGACC CAGGACCACA GAAGACCTTT TTGCAGCTAT TCACAGGTCT
    AAGAGGAAAG TCCTTGGCCG TAAAGATTCA GAGGATGATC ACTCCCGAAA CCATTCTCCC
    TCCCCACCAG TGACACCTAC TGGTGCCGCC CCCAGCCTGG CCTCCCAGAA GCAAGTGGGG
    TCCATTCAGA GAAGCGTCCG GAAGAGCAGC ACCAGCAGTG ACAACTTCAA AGCTCTGCTG
    CTGAAAAAGG GGAGCCGCTC GGATACCAGC GCCCGCATGT CAGCTGCAGA GATGCTCAAA
    AACACAGACC CTAGATTCCA GAGGTCAAGG TCGGAGCCAT CGCCAGACAC GCCCGAGAGC
    CCGTCAAACT GCTCCCCGAG CAAGAACAGG AGGGCGCAGG AGGAGTGGGC CAAGACCGAA
    GGCTTGATGC CTCGGAGTCT GTCCTTCTCC GGCCCCAGGT ACGGCCGCAG TCGAACGCCG
    CCTTCTGCCG CCAGCAGCAG GTACAGCGTG CGGAACCGGA TCCAGAGCAG CCCCATGACC
    GTCATCTCGG AGGGCGAAGG GGAAGCCACG GAGCCCATAG ACAGCACGGC TCTTCGGGCC
    CCGGGTGCTA CAAGGGGGTG TTCGCTGGAT GGACTGGCAG GGGGTGGTGT GGATGAGGGC
    AGCCTGCTCT GTGCCGGGGG ACCCGCTGCC CCGCTGGAGG CCCAGGCTCC TGGTCCGGCA
    GTGGGGCTGC CCAGCGCAGA GGGCAGGGGC CCTTCAGAAC AGCGAGGCGG TCTCGCCGGG
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852366.1
    CDS1103..5014
    Translation

    Target ORF information:

    RefSeq Version XM_024996598.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGACAGC GCTCAGAAAC CAGGGATTCC AGCTGTCAGA CTGAGGAAGT GAAGGTCGTA 
    CCACCTTCAA TGAGAAGAAT CAGAGCCCAG AAGGGGCAAG GCATTGCTGC TCAGATGAGT
    CACTTCTCAG GCTCCTCTGG GAACATGTCA GTGCTGAGTG ACTCTGCCGG GGTCGTGTTC
    CCTTCCCGCC TCAACAGCGA TGCTGGTTTT CACAGTCTTC CCCGGTCTGG AGCAAGGGCA
    AATGTTCAGG CCCTGGAGCC ACGGCTGGGC GCCCTGGGCC CTGCGGAAGA CCTGGGTGGC
    GCTTTCCCCT ACCAGCAGGG TCACCCACAA GTAGATGAAG ACTTTGGGCA TCTAGGAGCT
    GCCCCGAGGA TGGGAACACT GTTGAGGCCC AAATCTCAGG AGCTGAGGCA CTTCGAGGGT
    GAAAATGTAA CCAGCCCTGC GTGTGTGGTG TCTCCTCACA CGAGCTACTC CACCAGCATC
    ATCCCAAATG CCACGCTCTC CTCCTCTTCA GAGATCATTG TGATTCACAC TGCTCAGAGC
    TCAGGGCAGC TGGACAGTAA AATTACCAGC TCATCTTCAT ACTCAAAGGT CAGATCCAGA
    GACCACCTCC TCTCCAGGCA GAAAGATGGT CATCATTCGT CCAGTGCAAC CACACTGCTG
    TCCCTCTGTG ACTCCGAAGT CTCTCTAAAT ACACCAGCAA ATCGGGAGAA TGGGTCCCAG
    GCCATGGCCT ATAACTGTAG AAATAACCTT AGCATCCCAG CCCACCCCCA GGATGTGGAC
    AACAAGAGTG AGTCCAGTTA TTCAGGCAGC CGGGGCCAGG GTGGTAGCGT GGAGCCCTGG
    GAATATGGTG GCTCAGGTAG TGGGCGGGCA TCCCCACTGA AGCCACATTT GGCAGCTCCT
    GGTTACTCCA CTCCTGTAAG CAACACGAGC AGCTGCAGTT TGGACCAGAC CTCTAACAAG
    GATGATACCA GGTCTGTGTA TTCAGAGGAC CACGATGGCT ACTCCCCTAC TCTGCACCCG
    GACTCTGGAC ACAGACCTGG GGATCTGTGC AATAGTAATG ATGGCTTTGG GAACCCCAGG
    CACAGCGTGG TCAATGTTTT TGATGGAAGA GCTCAGAGAC ACCAAGGGGA CCGGTCCAAT
    TGCCAAGACA AATCCCTCGC GCGAAGCATT TCTTTGAAGA AAGCCAAGAA GCCTCCCCTG
    CCACCCTCTC GGACGGACTC TCTGCGCAGG GTTCCCAAGA AGAACGTCCA GTCGAACGGG
    CAGGTGCTGA ACGAGAGCCT GATCGCCTCT CTCCAGCACT CGCTGCAGCT GAACCTCCCG
    GGCAAGGGCG GCAGCTCGCC CTCCCAGAGC CCCTGCAGTG ATTTCGAAGA ACCGTGGCTG
    CCTCGCTCCC GCAGCCAGAG CACTGTGAGC GCCGGCAGCA GCCTAACCTC CGCCACGGCC
    CCCAACGTCT ACTCCGTGTG CGGGCTCACC CCGGCACAGA GCGACACGAG CAGCGTCAAG
    TCCGAGTACA CAGACCCGTG GGGTTACTAC ATTGACTACA CGGGAGTACA GGAAGATCAG
    GGGAACGCAG GCAGGGCCGG TCCGGCCGGC AGCGCAGCAT CACCTGGAAG TGGGCCGGGC
    CGCCATCTCC TGGAGGGGTC CCGGGCTGCA GTACCCCCAG TGCCCGGTGG CGCCGTCAAA
    CCAAAGATCA CATCACCGGA GAAGTCGCAC AGAGTCACTT CTCCATCCAG TGGGTATTCC
    AGTCAGTCGA ACACGCCCAC CGCTCTCACC CCTGTGCCTG TGTTCTTAAA ATCAATGTCA
    CCAGCCAATG GGAAGGGAAA GCCCAAACCC AAGGTGCCAG AAAGGAAGTC GTCTCTTGTA
    TCTTCAGTAT CCGTCTCCTC CTCCTCCACG TCTCTTTCCT CGAATACTTC CACTGAAGGA
    AGCGGGACGG TGAAGAAGCT GGATTCAGCG CTGGTGTCTC CGCTCGCGGC TGCTCCTCAC
    ACCTCTCCTC CAGCCCCTTG TCCCGCTGAC AAGTCTCCTT TCCTCCCTCC CCCGCCACCT
    CTGGCAGATT TCTCCGAGGG CTCACCTCTG CCTCGCTCTC CCCTCTTCCC CCCTCCGCCA
    CCAGAAGTGC TCACTCCCTT CTGTGCCCCC ACCGACGCCT GCTTTCCTCC GCCCCCTACA
    GCACTGAGCG CCCCTTTTCT GGATTCATCT GACTCCCTGC CACCTCCGCC ACCTGCCTTA
    ACCCCTTCAG TGCCCCCGCC TGCCCCACCG CTTGACCCCA AATTGATGAA AGATGCCAGG
    CCTTCTTTCA AGAAACCTGG CCAGCCAGAG CCCTCCCGGG AGGCCTGCAG ACAGCCTTCC
    ATCAAGGAGG AGGGCAGCAG ACTCCCCATG CCCCTGATCA CCACCGAAGC GTTGCAAATG
    GTGCAGTTGA GGCCGGTGAA GAAGAACTCA GGAACCGAGG TAGCCCCCTT ACAGGAACAC
    GCATCTCAGG AAAAACGAAC TCCGATTGTT TCCCAGTATC ATTTAAAGCC ATCTGCTTTC
    CTGAAATCCC GAAATAGCAT ACATGAAATG GAGAGTGAAA GCCAGCCTGC CTCCGCGACG
    AGCTCGCTCC CGACTCCTGC CAAGAGCTCG ACTCAGGGTC ACCAGGACAG TGCAGCCGAG
    CATGGCCTCC AGAGCCACAG CCCGGCCGGG GAGGCGGAGG CCGGAGCGGG TGCCACCCCG
    CCCCTCGGCC CGTCGCCCAG CAGGAAGCCG CCCCCCATTT CCAAGAAGCC CAAACTGTTC
    CTCGTGGTGT CACCTCCGCA GAGGGATTTC ACGGCGGAGC CCGCAGAGAA CGTGAGCGTG
    GCGTCTCCCA GCCCCACGAG CGGAGAGGCA CGAGAGAGGG GTGCAGCAGG GGCTGGTTCC
    GATGACACCG ACTCTGGCAG CTCGGTGGTT CTTGTGGGAG GAGCTGCAGG ATCCGAGTCC
    CCGGGCAGAG TGGAAGCCAA TGTCCCCATG GTGCCAGCCC CTGCGGAGGA GCCGGGTGCC
    AGGAACGGTG TGGAGCGCTG CCTGCCTCCG CAGGACCCAG GGCCTGGGGT GCCGGAGGCT
    GAGACAGCCA CTTCTTCTTC AGAGACCTGT GACTTCCCTA AGGACGACGG GGGTGATGAG
    GTGATGACCC CCAGTAGACC CAGGACCACA GAAGACCTTT TTGCAGCTAT TCACAGGTCT
    AAGAGGAAAG TCCTTGGCCG TAAAGATTCA GAGGATGATC ACTCCCGAAA CCATTCTCCC
    TCCCCACCAG TGACACCTAC TGGTGCCGCC CCCAGCCTGG CCTCCCAGAA GCAAGTGGGG
    TCCATTCAGA GAAGCGTCCG GAAGAGCAGC ACCAGCAGTG ACAACTTCAA AGCTCTGCTG
    CTGAAAAAGG GGAGCCGCTC GGATACCAGC GCCCGCATGT CAGCTGCAGA GATGCTCAAA
    AACACAGACC CTAGATTCCA GAGGTCAAGG TCGGAGCCAT CGCCAGACAC GCCCGAGAGC
    CCGTCAAACT GCTCCCCGAG CAAGAACAGG AGGGCGCAGG AGGAGTGGGC CAAGACCGAA
    GGCTTGATGC CTCGGAGTCT GTCCTTCTCC GGCCCCAGGT ACGGCCGCAG TCGAACGCCG
    CCTTCTGCCG CCAGCAGCAG GTACAGCGTG CGGAACCGGA TCCAGAGCAG CCCCATGACC
    GTCATCTCGG AGGGCGAAGG GGAAGCCACG GAGCCCATAG ACAGCACGGC TCTTCGGGCC
    CCGGGTGCTA CAAGGGGGTG TTCGCTGGAT GGACTGGCAG GGGGTGGTGT GGATGAGGGC
    AGCCTGCTCT GTGCCGGGGG ACCCGCTGCC CCGCTGGAGG CCCAGGCTCC TGGTCCGGCA
    GTGGGGCTGC CCAGCGCAGA GGGCAGGGGC CCTTCAGAAC AGCGAGGCGG TCTCGCCGGG
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39149
    Clone ID Related Accession (Same CDS sequence) NM_001191538.1
    Accession Version NM_001191538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-02
    Organism Bos taurus(cattle)
    Product NHS-like protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000009.1. On Apr 20, 2011 this sequence version replaced XM_002705214.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGGTCT TTATTAATAC AAAGATCAAG TCTTTGATTA AACTTTTTAA GAAGAAAACT 
    GTTTCCAACC TAGATGAGGA GAGTCGATGG ACGGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTCCC CACCACAAGA CCCCCCTGCG TGGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGTGATA AGTTACGGCG GGATGGCTAT
    CGGAGTTCTC AGTACTATTC CCAAGGACCC ACCTTTGCAG CCAACTCCAG TCCCCTCTGT
    GATGATTACC AAGATGAGGA TGAAGAAACA GATCCAAAGT GTTCTGTCTC TTCATCAGAA
    GAGGAAAGAC TTACTTCCAC CAGGAGACCT AAGACGCCAA CGTCAAGTGA CTGCTCTGAC
    CTTCATACTC AAACCAACTG GACCAAGTCA CTTCCCTTGC CAACACCGGA AGAGAAGATG
    CGGCAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTTCCTA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAGCAT ACAGAAGGAG
    CTAGCTTCGG GAGCCACTGG CCAAGATGAT GTTGGTGGTC ACTCAGCATA CACCCCAGAC
    CACTACTCTA CACTCGGAAG GCTCGACAGC TATCGATCTA TGGGACAGCG CTCAGAAACC
    AGGGATTCCA GCTGTCAGAC TGAGGAAGTG AAGGTCGTAC CACCTTCAAT GAGAAGAATC
    AGAGCCCAGA AGGGGCAAGG CATTGCTGCT CAGATGAGTC ACTTCTCAGG CTCCTCTGGG
    AACATGTCAG TGCTGAGTGA CTCTGCCGGG GTCGTGTTCC CTTCCCGCCT CAACAGCGAT
    GCTGGTTTTC ACAGTCTTCC CCGGTCTGGA GCAAGGGCAA ATGTTCAGGC CCTGGAGCCA
    CGGCTGGGCG CCCTGGGCCC TGCGGAAGAC CTGGGTGGCG CTTTCCCCTA CCAGCAGGGT
    CACCCACAAG TAGATGAAGA CTTTGGGCAT CTAGGAGCTG CCCCGAGGAT GGGAACACTG
    TTGAGGCCCA AATCTCAGGA GCTGAGGCAC TTCGAGGGTG AAAATGTAAC CAGCCCTGCG
    TGTGTGGTGT CTCCTCACAC GAGCTACTCC ACCAGCATCA TCCCAAATGC CACGCTCTCC
    TCCTCTTCAG AGATCATTGT GATTCACACT GCTCAGAGCT CAGGGCAGCT GGACAGTAAA
    ATTACCAGCT CATCTTCATA CTCAAAGGTC AGATCCAGAG ACCACCTCCT CTCCAGGCAG
    AAAGATGGTC ATCATTCGTC CAGTGCAACC ACACTGCTGT CCCTCTGTGA CTCCGAAGTC
    TCTCTAAATA CACCAGCAAA TCGGGAGAAT GGGTCCCAGG CCATGGCCTA TAACTGTAGA
    AATAACCTTA GCATCCCAGC CCACCCCCAG GATGTGGACA ACAAGAGTGA GTCCAGTTAT
    TCAGGCAGCC GGGGCCAGGG TGGTAGCGTG GAGCCCTGGG AATATGGTGG CTCAGGTAGT
    GGGCGGGCAT CCCCACTGAA GCCACATTTG GCAGCTCCTG GTTACTCCAC TCCTGTAAGC
    AACACGAGCA GCTGCAGTTT GGACCAGACC TCTAACAAGG ATGATACCAG GTCTGTGTAT
    TCAGAGGACC ACGATGGCTA CTCCCCTACT CTGCACCCGG ACTCTGGACA CAGACCTGGG
    GATCTGTGCA ATAGTAATGA TGGCTTTGGG AACCCCAGGC ACAGCGTGGT CAATGTTTTT
    GATGGAAGAG CTCAGAGACA CCAAGGGGAC CGGTCCAATT GCCAAGACAA ATCCCTCGCG
    CGAAGCATTT CTTTGAAGAA AGCCAAGAAG CCTCCCCTGC CACCCTCTCG GACGGACTCT
    CTGCGCAGGG TTCCCAAGAA GAACGTCCAG TCGAACGGGC AGGTGCTGAA CGAGAGCCTG
    ATCGCCTCTC TCCAGCACTC GCTGCAGCTG AACCTCCCGG GCAAGGGCGG CAGCTCGCCC
    TCCCAGAGCC CCTGCAGTGA TTTCGAAGAA CCGTGGCTGC CTCGCTCCCG CAGCCAGAGC
    ACTGTGAGCG CCGGCAGCAG CCTAACCTCC GCCACGGCCC CCAACGTCTA CTCCGTGTGC
    GGGCTCACCC CGGCACAGAG CGACACGAGC AGCGTCAAGT CCGAGTACAC AGACCCGTGG
    GGTTACTACA TTGACTACAC GGGAGTACAG GAAGATCAGG GGAACGCAGG CAGGGCCGGT
    CCGGCCGGCA GCGCAGCATC ACCTGGAAGT GGGCCGGGCC GCCATCTCCT GGAGGGGTCC
    CGGGCTGCAG TACCCCCAGT GCCCGGTGGC GCCGTCAAAC CAAAGATCAC ATCACCGGAG
    AAGTCGCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GTCAGTCGAA CACGCCCACC
    GCTCTCACCC CTGTGCCTGT GTTCTTAAAA TCAATGTCAC CAGCCAATGG GAAGGGAAAG
    CCCAAACCCA AGGTGCCAGA AAGGAAGTCG TCTCTTGTAT CTTCAGTATC CGTCTCCTCC
    TCCTCCACGT CTCTTTCCTC GAATACTTCC ACTGAAGGAA GCGGGACGGT GAAGAAGCTG
    GATTCAGCGC TGGTGTCTCC GCTCGCGGCT GCTCCTCACA CCTCTCCTCC AGCCCCTTGT
    CCCGCTGACA AGTCTCCTTT CCTCCCTCCC CCGCCACCTC TGGCAGATTT CTCCGAGGGC
    TCACCTCTGC CTCGCTCTCC CCTCTTCCCC CCTCCGCCAC CAGAAGTGCT CACTCCCTTC
    TGTGCCCCCA CCGACGCCTG CTTTCCTCCG CCCCCTACAG CACTGAGCGC CCCTTTTCTG
    GATTCATCTG ACTCCCTGCC ACCTCCGCCA CCTGCCTTAA CCCCTTCAGT GCCCCCGCCT
    GCCCCACCGC TTGACCCCAA ATTGATGAAA GATGCCAGGC CTTCTTTCAA GAAACCTGGC
    CAGCCAGAGC CCTCCCGGGA GGCCTGCAGA CAGCCTTCCA TCAAGGAGGA GGGCAGCAGA
    CTCCCCATGC CCCTGATCAC CACCGAAGCG TTGCAAATGG TGCAGTTGAG GCCGGTGAAG
    AAGAACTCAG GAACCGAGGT AGCCCCCTTA CAGGAACACG CATCTCAGGA AAAACGAACT
    CCGATTGTTT CCCAGTATCA TTTAAAGCCA TCTGCTTTCC TGAAATCCCG AAATAGCATA
    CATGAAATGG AGAGTGAAAG CCAGCCTGCC TCCGCGACGA GCTCGCTCCC GACTCCTGCC
    AAGAGCTCGA CTCAGGGTCA CCAGGACAGT GCAGCCGAGC ATGGCCTCCA GAGCCACAGC
    CCGGCCGGGG AGGCGGAGGC CGGAGCGGGT GCCACCCCGC CCCTCGGCCC GTCGCCCAGC
    AGGAAGCCGC CCCCCATTTC CAAGAAGCCC AAACTGTTCC TCGTGGTGTC ACCTCCGCAG
    AGGGATTTCA CGGCGGAGCC CGCAGAGAAC GTGAGCGTGG CGTCTCCCAG CCCCACGAGC
    GGAGAGGCAC GAGAGAGGGG TGCAGCAGGG GCTGGTTCCG ATGACACCGA CTCTGGCAGC
    TCGGTGGTTC TTGTGGGAGG AGCTGCAGGA TCCGAGTCCC CGGGCAGAGT GGAAGCCAAT
    GTCCCCATGG TGCCAGCCCC TGCGGAGGAG CCGGGTGCCA GGAACGGTGT GGAGCGCTGC
    CTGCCTCCGC AGGACCCAGG GCCTGGGGTG CCGGAGGCTG AGACAGCCAC TTCTTCTTCA
    GAGACCTGTG ACTTCCCTAA GGACGACGGG GGTGATGAGG TGATGACCCC CAGTAGACCC
    AGGACCACAG AAGACCTTTT TGCAGCTATT CACAGGTCTA AGAGGAAAGT CCTTGGCCGT
    AAAGATTCAG AGGATGATCA CTCCCGAAAC CATTCTCCCT CCCCACCAGT GACACCTACT
    GGTGCCGCCC CCAGCCTGGC CTCCCAGAAG CAAGTGGGGT CCATTCAGAG AAGCGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TGAAAAAGGG GAGCCGCTCG
    GATACCAGCG CCCGCATGTC AGCTGCAGAG ATGCTCAAAA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CGGAGCCATC GCCAGACACG CCCGAGAGCC CGTCAAACTG CTCCCCGAGC
    AAGAACAGGA GGGCGCAGGA GGAGTGGGCC AAGACCGAAG GCTTGATGCC TCGGAGTCTG
    TCCTTCTCCG GCCCCAGGTA CGGCCGCAGT CGAACGCCGC CTTCTGCCGC CAGCAGCAGG
    TACAGCGTGC GGAACCGGAT CCAGAGCAGC CCCATGACCG TCATCTCGGA GGGCGAAGGG
    GAAGCCACGG AGCCCATAGA CAGCACGGCT CTTCGGGCCC CGGGTGCTAC AAGGGGGTGT
    TCGCTGGATG GACTGGCAGG GGGTGGTGTG GATGAGGGCA GCCTGCTCTG TGCCGGGGGA
    CCCGCTGCCC CGCTGGAGGC CCAGGCTCCT GGTCCGGCAG TGGGGCTGCC CAGCGCAGAG
    GGCAGGGGCC CTTCAGAACA GCGAGGCGGT CTCGCCGGGG ATAAGAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178467.1
    CDS1..4671
    Translation

    Target ORF information:

    RefSeq Version NM_001191538.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGGTCT TTATTAATAC AAAGATCAAG TCTTTGATTA AACTTTTTAA GAAGAAAACT 
    GTTTCCAACC TAGATGAGGA GAGTCGATGG ACGGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTCCC CACCACAAGA CCCCCCTGCG TGGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGTGATA AGTTACGGCG GGATGGCTAT
    CGGAGTTCTC AGTACTATTC CCAAGGACCC ACCTTTGCAG CCAACTCCAG TCCCCTCTGT
    GATGATTACC AAGATGAGGA TGAAGAAACA GATCCAAAGT GTTCTGTCTC TTCATCAGAA
    GAGGAAAGAC TTACTTCCAC CAGGAGACCT AAGACGCCAA CGTCAAGTGA CTGCTCTGAC
    CTTCATACTC AAACCAACTG GACCAAGTCA CTTCCCTTGC CAACACCGGA AGAGAAGATG
    CGGCAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTTCCTA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAGCAT ACAGAAGGAG
    CTAGCTTCGG GAGCCACTGG CCAAGATGAT GTTGGTGGTC ACTCAGCATA CACCCCAGAC
    CACTACTCTA CACTCGGAAG GCTCGACAGC TATCGATCTA TGGGACAGCG CTCAGAAACC
    AGGGATTCCA GCTGTCAGAC TGAGGAAGTG AAGGTCGTAC CACCTTCAAT GAGAAGAATC
    AGAGCCCAGA AGGGGCAAGG CATTGCTGCT CAGATGAGTC ACTTCTCAGG CTCCTCTGGG
    AACATGTCAG TGCTGAGTGA CTCTGCCGGG GTCGTGTTCC CTTCCCGCCT CAACAGCGAT
    GCTGGTTTTC ACAGTCTTCC CCGGTCTGGA GCAAGGGCAA ATGTTCAGGC CCTGGAGCCA
    CGGCTGGGCG CCCTGGGCCC TGCGGAAGAC CTGGGTGGCG CTTTCCCCTA CCAGCAGGGT
    CACCCACAAG TAGATGAAGA CTTTGGGCAT CTAGGAGCTG CCCCGAGGAT GGGAACACTG
    TTGAGGCCCA AATCTCAGGA GCTGAGGCAC TTCGAGGGTG AAAATGTAAC CAGCCCTGCG
    TGTGTGGTGT CTCCTCACAC GAGCTACTCC ACCAGCATCA TCCCAAATGC CACGCTCTCC
    TCCTCTTCAG AGATCATTGT GATTCACACT GCTCAGAGCT CAGGGCAGCT GGACAGTAAA
    ATTACCAGCT CATCTTCATA CTCAAAGGTC AGATCCAGAG ACCACCTCCT CTCCAGGCAG
    AAAGATGGTC ATCATTCGTC CAGTGCAACC ACACTGCTGT CCCTCTGTGA CTCCGAAGTC
    TCTCTAAATA CACCAGCAAA TCGGGAGAAT GGGTCCCAGG CCATGGCCTA TAACTGTAGA
    AATAACCTTA GCATCCCAGC CCACCCCCAG GATGTGGACA ACAAGAGTGA GTCCAGTTAT
    TCAGGCAGCC GGGGCCAGGG TGGTAGCGTG GAGCCCTGGG AATATGGTGG CTCAGGTAGT
    GGGCGGGCAT CCCCACTGAA GCCACATTTG GCAGCTCCTG GTTACTCCAC TCCTGTAAGC
    AACACGAGCA GCTGCAGTTT GGACCAGACC TCTAACAAGG ATGATACCAG GTCTGTGTAT
    TCAGAGGACC ACGATGGCTA CTCCCCTACT CTGCACCCGG ACTCTGGACA CAGACCTGGG
    GATCTGTGCA ATAGTAATGA TGGCTTTGGG AACCCCAGGC ACAGCGTGGT CAATGTTTTT
    GATGGAAGAG CTCAGAGACA CCAAGGGGAC CGGTCCAATT GCCAAGACAA ATCCCTCGCG
    CGAAGCATTT CTTTGAAGAA AGCCAAGAAG CCTCCCCTGC CACCCTCTCG GACGGACTCT
    CTGCGCAGGG TTCCCAAGAA GAACGTCCAG TCGAACGGGC AGGTGCTGAA CGAGAGCCTG
    ATCGCCTCTC TCCAGCACTC GCTGCAGCTG AACCTCCCGG GCAAGGGCGG CAGCTCGCCC
    TCCCAGAGCC CCTGCAGTGA TTTCGAAGAA CCGTGGCTGC CTCGCTCCCG CAGCCAGAGC
    ACTGTGAGCG CCGGCAGCAG CCTAACCTCC GCCACGGCCC CCAACGTCTA CTCCGTGTGC
    GGGCTCACCC CGGCACAGAG CGACACGAGC AGCGTCAAGT CCGAGTACAC AGACCCGTGG
    GGTTACTACA TTGACTACAC GGGAGTACAG GAAGATCAGG GGAACGCAGG CAGGGCCGGT
    CCGGCCGGCA GCGCAGCATC ACCTGGAAGT GGGCCGGGCC GCCATCTCCT GGAGGGGTCC
    CGGGCTGCAG TACCCCCAGT GCCCGGTGGC GCCGTCAAAC CAAAGATCAC ATCACCGGAG
    AAGTCGCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GTCAGTCGAA CACGCCCACC
    GCTCTCACCC CTGTGCCTGT GTTCTTAAAA TCAATGTCAC CAGCCAATGG GAAGGGAAAG
    CCCAAACCCA AGGTGCCAGA AAGGAAGTCG TCTCTTGTAT CTTCAGTATC CGTCTCCTCC
    TCCTCCACGT CTCTTTCCTC GAATACTTCC ACTGAAGGAA GCGGGACGGT GAAGAAGCTG
    GATTCAGCGC TGGTGTCTCC GCTCGCGGCT GCTCCTCACA CCTCTCCTCC AGCCCCTTGT
    CCCGCTGACA AGTCTCCTTT CCTCCCTCCC CCGCCACCTC TGGCAGATTT CTCCGAGGGC
    TCACCTCTGC CTCGCTCTCC CCTCTTCCCC CCTCCGCCAC CAGAAGTGCT CACTCCCTTC
    TGTGCCCCCA CCGACGCCTG CTTTCCTCCG CCCCCTACAG CACTGAGCGC CCCTTTTCTG
    GATTCATCTG ACTCCCTGCC ACCTCCGCCA CCTGCCTTAA CCCCTTCAGT GCCCCCGCCT
    GCCCCACCGC TTGACCCCAA ATTGATGAAA GATGCCAGGC CTTCTTTCAA GAAACCTGGC
    CAGCCAGAGC CCTCCCGGGA GGCCTGCAGA CAGCCTTCCA TCAAGGAGGA GGGCAGCAGA
    CTCCCCATGC CCCTGATCAC CACCGAAGCG TTGCAAATGG TGCAGTTGAG GCCGGTGAAG
    AAGAACTCAG GAACCGAGGT AGCCCCCTTA CAGGAACACG CATCTCAGGA AAAACGAACT
    CCGATTGTTT CCCAGTATCA TTTAAAGCCA TCTGCTTTCC TGAAATCCCG AAATAGCATA
    CATGAAATGG AGAGTGAAAG CCAGCCTGCC TCCGCGACGA GCTCGCTCCC GACTCCTGCC
    AAGAGCTCGA CTCAGGGTCA CCAGGACAGT GCAGCCGAGC ATGGCCTCCA GAGCCACAGC
    CCGGCCGGGG AGGCGGAGGC CGGAGCGGGT GCCACCCCGC CCCTCGGCCC GTCGCCCAGC
    AGGAAGCCGC CCCCCATTTC CAAGAAGCCC AAACTGTTCC TCGTGGTGTC ACCTCCGCAG
    AGGGATTTCA CGGCGGAGCC CGCAGAGAAC GTGAGCGTGG CGTCTCCCAG CCCCACGAGC
    GGAGAGGCAC GAGAGAGGGG TGCAGCAGGG GCTGGTTCCG ATGACACCGA CTCTGGCAGC
    TCGGTGGTTC TTGTGGGAGG AGCTGCAGGA TCCGAGTCCC CGGGCAGAGT GGAAGCCAAT
    GTCCCCATGG TGCCAGCCCC TGCGGAGGAG CCGGGTGCCA GGAACGGTGT GGAGCGCTGC
    CTGCCTCCGC AGGACCCAGG GCCTGGGGTG CCGGAGGCTG AGACAGCCAC TTCTTCTTCA
    GAGACCTGTG ACTTCCCTAA GGACGACGGG GGTGATGAGG TGATGACCCC CAGTAGACCC
    AGGACCACAG AAGACCTTTT TGCAGCTATT CACAGGTCTA AGAGGAAAGT CCTTGGCCGT
    AAAGATTCAG AGGATGATCA CTCCCGAAAC CATTCTCCCT CCCCACCAGT GACACCTACT
    GGTGCCGCCC CCAGCCTGGC CTCCCAGAAG CAAGTGGGGT CCATTCAGAG AAGCGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TGAAAAAGGG GAGCCGCTCG
    GATACCAGCG CCCGCATGTC AGCTGCAGAG ATGCTCAAAA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CGGAGCCATC GCCAGACACG CCCGAGAGCC CGTCAAACTG CTCCCCGAGC
    AAGAACAGGA GGGCGCAGGA GGAGTGGGCC AAGACCGAAG GCTTGATGCC TCGGAGTCTG
    TCCTTCTCCG GCCCCAGGTA CGGCCGCAGT CGAACGCCGC CTTCTGCCGC CAGCAGCAGG
    TACAGCGTGC GGAACCGGAT CCAGAGCAGC CCCATGACCG TCATCTCGGA GGGCGAAGGG
    GAAGCCACGG AGCCCATAGA CAGCACGGCT CTTCGGGCCC CGGGTGCTAC AAGGGGGTGT
    TCGCTGGATG GACTGGCAGG GGGTGGTGTG GATGAGGGCA GCCTGCTCTG TGCCGGGGGA
    CCCGCTGCCC CGCTGGAGGC CCAGGCTCCT GGTCCGGCAG TGGGGCTGCC CAGCGCAGAG
    GGCAGGGGCC CTTCAGAACA GCGAGGCGGT CTCGCCGGGG ATAAGAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22716
    Clone ID Related Accession (Same CDS sequence) XM_005210988.4 , XM_005210988.3
    Accession Version XM_005210988.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT
    TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA
    GTCCCTGACA GCATACAGAA GGAGCTAGCT TCGGGAGCCA CTGGCCAAGA TGATGTTGGT
    GGTCACTCAG CATACACCCC AGACCACTAC TCTACACTCG GAAGGCTCGA CAGCTATCGA
    TCTATGGGAC AGCGCTCAGA AACCAGGGAT TCCAGCTGTC AGACTGAGGA AGTGAAGGTC
    GTACCACCTT CAATGAGAAG AATCAGAGCC CAGAAGGGGC AAGGCATTGC TGCTCAGATG
    AGTCACTTCT CAGGCTCCTC TGGGAACATG TCAGTGCTGA GTGACTCTGC CGGGGTCGTG
    TTCCCTTCCC GCCTCAACAG CGATGCTGGT TTTCACAGTC TTCCCCGGTC TGGAGCAAGG
    GCAAATGTTC AGGCCCTGGA GCCACGGCTG GGCGCCCTGG GCCCTGCGGA AGACCTGGGT
    GGCGCTTTCC CCTACCAGCA GGGTCACCCA CAAGTAGATG AAGACTTTGG GCATCTAGGA
    GCTGCCCCGA GGATGGGAAC ACTGTTGAGG CCCAAATCTC AGGAGCTGAG GCACTTCGAG
    GGTGAAAATG TAACCAGCCC TGCGTGTGTG GTGTCTCCTC ACACGAGCTA CTCCACCAGC
    ATCATCCCAA ATGCCACGCT CTCCTCCTCT TCAGAGATCA TTGTGATTCA CACTGCTCAG
    AGCTCAGGGC AGCTGGACAG TAAAATTACC AGCTCATCTT CATACTCAAA GGTCAGATCC
    AGAGACCACC TCCTCTCCAG GCAGAAAGAT GGTCATCATT CGTCCAGTGC AACCACACTG
    CTGTCCCTCT GTGACTCCGA AGTCTCTCTA AATACACCAG CAAATCGGGA GAATGGGTCC
    CAGGCCATGG CCTATAACTG TAGAAATAAC CTTAGCATCC CAGCCCACCC CCAGGATGTG
    GACAACAAGA GTGAGTCCAG TTATTCAGGC AGCCGGGGCC AGGGTGGTAG CGTGGAGCCC
    TGGGAATATG GTGGCTCAGG TAGTGGGCGG GCATCCCCAC TGAAGCCACA TTTGGCAGCT
    CCTGGTTACT CCACTCCTGT AAGCAACACG AGCAGCTGCA GTTTGGACCA GACCTCTAAC
    AAGGATGATA CCAGGTCTGT GTATTCAGAG GACCACGATG GCTACTCCCC TACTCTGCAC
    CCGGACTCTG GACACAGACC TGGGGATCTG TGCAATAGTA ATGATGGCTT TGGGAACCCC
    AGGCACAGCG TGGTCAATGT TTTTGATGGA AGAGCTCAGA GACACCAAGG GGACCGGTCC
    AATTGCCAAG ACAAATCCCT CGCGCGAAGC ATTTCTTTGA AGAAAGCCAA GAAGCCTCCC
    CTGCCACCCT CTCGGACGGA CTCTCTGCGC AGGGTTCCCA AGAAGAACGT CCAGTCGAAC
    GGGCAGGTGC TGAACGAGAG CCTGATCGCC TCTCTCCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGATTTCGA AGAACCGTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACTGTG AGCGCCGGCA GCAGCCTAAC CTCCGCCACG
    GCCCCCAACG TCTACTCCGT GTGCGGGCTC ACCCCGGCAC AGAGCGACAC GAGCAGCGTC
    AAGTCCGAGT ACACAGACCC GTGGGGTTAC TACATTGACT ACACGGGAGT ACAGGAAGAT
    CAGGGGAACG CAGGCAGGGC CGGTCCGGCC GGCAGCGCAG CATCACCTGG AAGTGGGCCG
    GGCCGCCATC TCCTGGAGGG GTCCCGGGCT GCAGTACCCC CAGTGCCCGG TGGCGCCGTC
    AAACCAAAGA TCACATCACC GGAGAAGTCG CACAGAGTCA CTTCTCCATC CAGTGGGTAT
    TCCAGTCAGT CGAACACGCC CACCGCTCTC ACCCCTGTGC CTGTGTTCTT AAAATCAATG
    TCACCAGCCA ATGGGAAGGG AAAGCCCAAA CCCAAGGTGC CAGAAAGGAA GTCGTCTCTT
    GTATCTTCAG TATCCGTCTC CTCCTCCTCC ACGTCTCTTT CCTCGAATAC TTCCACTGAA
    GGAAGCGGGA CGGTGAAGAA GCTGGATTCA GCGCTGGTGT CTCCGCTCGC GGCTGCTCCT
    CACACCTCTC CTCCAGCCCC TTGTCCCGCT GACAAGTCTC CTTTCCTCCC TCCCCCGCCA
    CCTCTGGCAG ATTTCTCCGA GGGCTCACCT CTGCCTCGCT CTCCCCTCTT CCCCCCTCCG
    CCACCAGAAG TGCTCACTCC CTTCTGTGCC CCCACCGACG CCTGCTTTCC TCCGCCCCCT
    ACAGCACTGA GCGCCCCTTT TCTGGATTCA TCTGACTCCC TGCCACCTCC GCCACCTGCC
    TTAACCCCTT CAGTGCCCCC GCCTGCCCCA CCGCTTGACC CCAAATTGAT GAAAGATGCC
    AGGCCTTCTT TCAAGAAACC TGGCCAGCCA GAGCCCTCCC GGGAGGCCTG CAGACAGCCT
    TCCATCAAGG AGGAGGGCAG CAGACTCCCC ATGCCCCTGA TCACCACCGA AGCGTTGCAA
    ATGGTGCAGT TGAGGCCGGT GAAGAAGAAC TCAGGAACCG AGGTAGCCCC CTTACAGGAA
    CACGCATCTC AGGAAAAACG AACTCCGATT GTTTCCCAGT ATCATTTAAA GCCATCTGCT
    TTCCTGAAAT CCCGAAATAG CATACATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCCGCG
    ACGAGCTCGC TCCCGACTCC TGCCAAGAGC TCGACTCAGG GTCACCAGGA CAGTGCAGCC
    GAGCATGGCC TCCAGAGCCA CAGCCCGGCC GGGGAGGCGG AGGCCGGAGC GGGTGCCACC
    CCGCCCCTCG GCCCGTCGCC CAGCAGGAAG CCGCCCCCCA TTTCCAAGAA GCCCAAACTG
    TTCCTCGTGG TGTCACCTCC GCAGAGGGAT TTCACGGCGG AGCCCGCAGA GAACGTGAGC
    GTGGCGTCTC CCAGCCCCAC GAGCGGAGAG GCACGAGAGA GGGGTGCAGC AGGGGCTGGT
    TCCGATGACA CCGACTCTGG CAGCTCGGTG GTTCTTGTGG GAGGAGCTGC AGGATCCGAG
    TCCCCGGGCA GAGTGGAAGC CAATGTCCCC ATGGTGCCAG CCCCTGCGGA GGAGCCGGGT
    GCCAGGAACG GTGTGGAGCG CTGCCTGCCT CCGCAGGACC CAGGGCCTGG GGTGCCGGAG
    GCTGAGACAG CCACTTCTTC TTCAGAGACC TGTGACTTCC CTAAGGACGA CGGGGGTGAT
    GAGGTGATGA CCCCCAGTAG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGG
    TCTAAGAGGA AAGTCCTTGG CCGTAAAGAT TCAGAGGATG ATCACTCCCG AAACCATTCT
    CCCTCCCCAC CAGTGACACC TACTGGTGCC GCCCCCAGCC TGGCCTCCCA GAAGCAAGTG
    GGGTCCATTC AGAGAAGCGT CCGGAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG
    CTGCTGAAAA AGGGGAGCCG CTCGGATACC AGCGCCCGCA TGTCAGCTGC AGAGATGCTC
    AAAAACACAG ACCCTAGATT CCAGAGGTCA AGGTCGGAGC CATCGCCAGA CACGCCCGAG
    AGCCCGTCAA ACTGCTCCCC GAGCAAGAAC AGGAGGGCGC AGGAGGAGTG GGCCAAGACC
    GAAGGCTTGA TGCCTCGGAG TCTGTCCTTC TCCGGCCCCA GGTACGGCCG CAGTCGAACG
    CCGCCTTCTG CCGCCAGCAG CAGGTACAGC GTGCGGAACC GGATCCAGAG CAGCCCCATG
    ACCGTCATCT CGGAGGGCGA AGGGGAAGCC ACGGAGCCCA TAGACAGCAC GGCTCTTCGG
    GCCCCGGGTG CTACAAGGGG GTGTTCGCTG GATGGACTGG CAGGGGGTGG TGTGGATGAG
    GGCAGCCTGC TCTGTGCCGG GGGACCCGCT GCCCCGCTGG AGGCCCAGGC TCCTGGTCCG
    GCAGTGGGGC TGCCCAGCGC AGAGGGCAGG GGCCCTTCAG AACAGCGAGG CGGTCTCGCC
    GGGGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211045.1
    CDS1500..6314
    Translation

    Target ORF information:

    RefSeq Version XM_005210988.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210988.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT
    TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA
    GTCCCTGACA GCATACAGAA GGAGCTAGCT TCGGGAGCCA CTGGCCAAGA TGATGTTGGT
    GGTCACTCAG CATACACCCC AGACCACTAC TCTACACTCG GAAGGCTCGA CAGCTATCGA
    TCTATGGGAC AGCGCTCAGA AACCAGGGAT TCCAGCTGTC AGACTGAGGA AGTGAAGGTC
    GTACCACCTT CAATGAGAAG AATCAGAGCC CAGAAGGGGC AAGGCATTGC TGCTCAGATG
    AGTCACTTCT CAGGCTCCTC TGGGAACATG TCAGTGCTGA GTGACTCTGC CGGGGTCGTG
    TTCCCTTCCC GCCTCAACAG CGATGCTGGT TTTCACAGTC TTCCCCGGTC TGGAGCAAGG
    GCAAATGTTC AGGCCCTGGA GCCACGGCTG GGCGCCCTGG GCCCTGCGGA AGACCTGGGT
    GGCGCTTTCC CCTACCAGCA GGGTCACCCA CAAGTAGATG AAGACTTTGG GCATCTAGGA
    GCTGCCCCGA GGATGGGAAC ACTGTTGAGG CCCAAATCTC AGGAGCTGAG GCACTTCGAG
    GGTGAAAATG TAACCAGCCC TGCGTGTGTG GTGTCTCCTC ACACGAGCTA CTCCACCAGC
    ATCATCCCAA ATGCCACGCT CTCCTCCTCT TCAGAGATCA TTGTGATTCA CACTGCTCAG
    AGCTCAGGGC AGCTGGACAG TAAAATTACC AGCTCATCTT CATACTCAAA GGTCAGATCC
    AGAGACCACC TCCTCTCCAG GCAGAAAGAT GGTCATCATT CGTCCAGTGC AACCACACTG
    CTGTCCCTCT GTGACTCCGA AGTCTCTCTA AATACACCAG CAAATCGGGA GAATGGGTCC
    CAGGCCATGG CCTATAACTG TAGAAATAAC CTTAGCATCC CAGCCCACCC CCAGGATGTG
    GACAACAAGA GTGAGTCCAG TTATTCAGGC AGCCGGGGCC AGGGTGGTAG CGTGGAGCCC
    TGGGAATATG GTGGCTCAGG TAGTGGGCGG GCATCCCCAC TGAAGCCACA TTTGGCAGCT
    CCTGGTTACT CCACTCCTGT AAGCAACACG AGCAGCTGCA GTTTGGACCA GACCTCTAAC
    AAGGATGATA CCAGGTCTGT GTATTCAGAG GACCACGATG GCTACTCCCC TACTCTGCAC
    CCGGACTCTG GACACAGACC TGGGGATCTG TGCAATAGTA ATGATGGCTT TGGGAACCCC
    AGGCACAGCG TGGTCAATGT TTTTGATGGA AGAGCTCAGA GACACCAAGG GGACCGGTCC
    AATTGCCAAG ACAAATCCCT CGCGCGAAGC ATTTCTTTGA AGAAAGCCAA GAAGCCTCCC
    CTGCCACCCT CTCGGACGGA CTCTCTGCGC AGGGTTCCCA AGAAGAACGT CCAGTCGAAC
    GGGCAGGTGC TGAACGAGAG CCTGATCGCC TCTCTCCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGATTTCGA AGAACCGTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACTGTG AGCGCCGGCA GCAGCCTAAC CTCCGCCACG
    GCCCCCAACG TCTACTCCGT GTGCGGGCTC ACCCCGGCAC AGAGCGACAC GAGCAGCGTC
    AAGTCCGAGT ACACAGACCC GTGGGGTTAC TACATTGACT ACACGGGAGT ACAGGAAGAT
    CAGGGGAACG CAGGCAGGGC CGGTCCGGCC GGCAGCGCAG CATCACCTGG AAGTGGGCCG
    GGCCGCCATC TCCTGGAGGG GTCCCGGGCT GCAGTACCCC CAGTGCCCGG TGGCGCCGTC
    AAACCAAAGA TCACATCACC GGAGAAGTCG CACAGAGTCA CTTCTCCATC CAGTGGGTAT
    TCCAGTCAGT CGAACACGCC CACCGCTCTC ACCCCTGTGC CTGTGTTCTT AAAATCAATG
    TCACCAGCCA ATGGGAAGGG AAAGCCCAAA CCCAAGGTGC CAGAAAGGAA GTCGTCTCTT
    GTATCTTCAG TATCCGTCTC CTCCTCCTCC ACGTCTCTTT CCTCGAATAC TTCCACTGAA
    GGAAGCGGGA CGGTGAAGAA GCTGGATTCA GCGCTGGTGT CTCCGCTCGC GGCTGCTCCT
    CACACCTCTC CTCCAGCCCC TTGTCCCGCT GACAAGTCTC CTTTCCTCCC TCCCCCGCCA
    CCTCTGGCAG ATTTCTCCGA GGGCTCACCT CTGCCTCGCT CTCCCCTCTT CCCCCCTCCG
    CCACCAGAAG TGCTCACTCC CTTCTGTGCC CCCACCGACG CCTGCTTTCC TCCGCCCCCT
    ACAGCACTGA GCGCCCCTTT TCTGGATTCA TCTGACTCCC TGCCACCTCC GCCACCTGCC
    TTAACCCCTT CAGTGCCCCC GCCTGCCCCA CCGCTTGACC CCAAATTGAT GAAAGATGCC
    AGGCCTTCTT TCAAGAAACC TGGCCAGCCA GAGCCCTCCC GGGAGGCCTG CAGACAGCCT
    TCCATCAAGG AGGAGGGCAG CAGACTCCCC ATGCCCCTGA TCACCACCGA AGCGTTGCAA
    ATGGTGCAGT TGAGGCCGGT GAAGAAGAAC TCAGGAACCG AGGTAGCCCC CTTACAGGAA
    CACGCATCTC AGGAAAAACG AACTCCGATT GTTTCCCAGT ATCATTTAAA GCCATCTGCT
    TTCCTGAAAT CCCGAAATAG CATACATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCCGCG
    ACGAGCTCGC TCCCGACTCC TGCCAAGAGC TCGACTCAGG GTCACCAGGA CAGTGCAGCC
    GAGCATGGCC TCCAGAGCCA CAGCCCGGCC GGGGAGGCGG AGGCCGGAGC GGGTGCCACC
    CCGCCCCTCG GCCCGTCGCC CAGCAGGAAG CCGCCCCCCA TTTCCAAGAA GCCCAAACTG
    TTCCTCGTGG TGTCACCTCC GCAGAGGGAT TTCACGGCGG AGCCCGCAGA GAACGTGAGC
    GTGGCGTCTC CCAGCCCCAC GAGCGGAGAG GCACGAGAGA GGGGTGCAGC AGGGGCTGGT
    TCCGATGACA CCGACTCTGG CAGCTCGGTG GTTCTTGTGG GAGGAGCTGC AGGATCCGAG
    TCCCCGGGCA GAGTGGAAGC CAATGTCCCC ATGGTGCCAG CCCCTGCGGA GGAGCCGGGT
    GCCAGGAACG GTGTGGAGCG CTGCCTGCCT CCGCAGGACC CAGGGCCTGG GGTGCCGGAG
    GCTGAGACAG CCACTTCTTC TTCAGAGACC TGTGACTTCC CTAAGGACGA CGGGGGTGAT
    GAGGTGATGA CCCCCAGTAG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGG
    TCTAAGAGGA AAGTCCTTGG CCGTAAAGAT TCAGAGGATG ATCACTCCCG AAACCATTCT
    CCCTCCCCAC CAGTGACACC TACTGGTGCC GCCCCCAGCC TGGCCTCCCA GAAGCAAGTG
    GGGTCCATTC AGAGAAGCGT CCGGAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG
    CTGCTGAAAA AGGGGAGCCG CTCGGATACC AGCGCCCGCA TGTCAGCTGC AGAGATGCTC
    AAAAACACAG ACCCTAGATT CCAGAGGTCA AGGTCGGAGC CATCGCCAGA CACGCCCGAG
    AGCCCGTCAA ACTGCTCCCC GAGCAAGAAC AGGAGGGCGC AGGAGGAGTG GGCCAAGACC
    GAAGGCTTGA TGCCTCGGAG TCTGTCCTTC TCCGGCCCCA GGTACGGCCG CAGTCGAACG
    CCGCCTTCTG CCGCCAGCAG CAGGTACAGC GTGCGGAACC GGATCCAGAG CAGCCCCATG
    ACCGTCATCT CGGAGGGCGA AGGGGAAGCC ACGGAGCCCA TAGACAGCAC GGCTCTTCGG
    GCCCCGGGTG CTACAAGGGG GTGTTCGCTG GATGGACTGG CAGGGGGTGG TGTGGATGAG
    GGCAGCCTGC TCTGTGCCGG GGGACCCGCT GCCCCGCTGG AGGCCCAGGC TCCTGGTCCG
    GCAGTGGGGC TGCCCAGCGC AGAGGGCAGG GGCCCTTCAG AACAGCGAGG CGGTCTCGCC
    GGGGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22716
    Clone ID Related Accession (Same CDS sequence) XM_005210988.4 , XM_005210988.3
    Accession Version XM_005210988.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT
    TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA
    GTCCCTGACA GCATACAGAA GGAGCTAGCT TCGGGAGCCA CTGGCCAAGA TGATGTTGGT
    GGTCACTCAG CATACACCCC AGACCACTAC TCTACACTCG GAAGGCTCGA CAGCTATCGA
    TCTATGGGAC AGCGCTCAGA AACCAGGGAT TCCAGCTGTC AGACTGAGGA AGTGAAGGTC
    GTACCACCTT CAATGAGAAG AATCAGAGCC CAGAAGGGGC AAGGCATTGC TGCTCAGATG
    AGTCACTTCT CAGGCTCCTC TGGGAACATG TCAGTGCTGA GTGACTCTGC CGGGGTCGTG
    TTCCCTTCCC GCCTCAACAG CGATGCTGGT TTTCACAGTC TTCCCCGGTC TGGAGCAAGG
    GCAAATGTTC AGGCCCTGGA GCCACGGCTG GGCGCCCTGG GCCCTGCGGA AGACCTGGGT
    GGCGCTTTCC CCTACCAGCA GGGTCACCCA CAAGTAGATG AAGACTTTGG GCATCTAGGA
    GCTGCCCCGA GGATGGGAAC ACTGTTGAGG CCCAAATCTC AGGAGCTGAG GCACTTCGAG
    GGTGAAAATG TAACCAGCCC TGCGTGTGTG GTGTCTCCTC ACACGAGCTA CTCCACCAGC
    ATCATCCCAA ATGCCACGCT CTCCTCCTCT TCAGAGATCA TTGTGATTCA CACTGCTCAG
    AGCTCAGGGC AGCTGGACAG TAAAATTACC AGCTCATCTT CATACTCAAA GGTCAGATCC
    AGAGACCACC TCCTCTCCAG GCAGAAAGAT GGTCATCATT CGTCCAGTGC AACCACACTG
    CTGTCCCTCT GTGACTCCGA AGTCTCTCTA AATACACCAG CAAATCGGGA GAATGGGTCC
    CAGGCCATGG CCTATAACTG TAGAAATAAC CTTAGCATCC CAGCCCACCC CCAGGATGTG
    GACAACAAGA GTGAGTCCAG TTATTCAGGC AGCCGGGGCC AGGGTGGTAG CGTGGAGCCC
    TGGGAATATG GTGGCTCAGG TAGTGGGCGG GCATCCCCAC TGAAGCCACA TTTGGCAGCT
    CCTGGTTACT CCACTCCTGT AAGCAACACG AGCAGCTGCA GTTTGGACCA GACCTCTAAC
    AAGGATGATA CCAGGTCTGT GTATTCAGAG GACCACGATG GCTACTCCCC TACTCTGCAC
    CCGGACTCTG GACACAGACC TGGGGATCTG TGCAATAGTA ATGATGGCTT TGGGAACCCC
    AGGCACAGCG TGGTCAATGT TTTTGATGGA AGAGCTCAGA GACACCAAGG GGACCGGTCC
    AATTGCCAAG ACAAATCCCT CGCGCGAAGC ATTTCTTTGA AGAAAGCCAA GAAGCCTCCC
    CTGCCACCCT CTCGGACGGA CTCTCTGCGC AGGGTTCCCA AGAAGAACGT CCAGTCGAAC
    GGGCAGGTGC TGAACGAGAG CCTGATCGCC TCTCTCCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGATTTCGA AGAACCGTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACTGTG AGCGCCGGCA GCAGCCTAAC CTCCGCCACG
    GCCCCCAACG TCTACTCCGT GTGCGGGCTC ACCCCGGCAC AGAGCGACAC GAGCAGCGTC
    AAGTCCGAGT ACACAGACCC GTGGGGTTAC TACATTGACT ACACGGGAGT ACAGGAAGAT
    CAGGGGAACG CAGGCAGGGC CGGTCCGGCC GGCAGCGCAG CATCACCTGG AAGTGGGCCG
    GGCCGCCATC TCCTGGAGGG GTCCCGGGCT GCAGTACCCC CAGTGCCCGG TGGCGCCGTC
    AAACCAAAGA TCACATCACC GGAGAAGTCG CACAGAGTCA CTTCTCCATC CAGTGGGTAT
    TCCAGTCAGT CGAACACGCC CACCGCTCTC ACCCCTGTGC CTGTGTTCTT AAAATCAATG
    TCACCAGCCA ATGGGAAGGG AAAGCCCAAA CCCAAGGTGC CAGAAAGGAA GTCGTCTCTT
    GTATCTTCAG TATCCGTCTC CTCCTCCTCC ACGTCTCTTT CCTCGAATAC TTCCACTGAA
    GGAAGCGGGA CGGTGAAGAA GCTGGATTCA GCGCTGGTGT CTCCGCTCGC GGCTGCTCCT
    CACACCTCTC CTCCAGCCCC TTGTCCCGCT GACAAGTCTC CTTTCCTCCC TCCCCCGCCA
    CCTCTGGCAG ATTTCTCCGA GGGCTCACCT CTGCCTCGCT CTCCCCTCTT CCCCCCTCCG
    CCACCAGAAG TGCTCACTCC CTTCTGTGCC CCCACCGACG CCTGCTTTCC TCCGCCCCCT
    ACAGCACTGA GCGCCCCTTT TCTGGATTCA TCTGACTCCC TGCCACCTCC GCCACCTGCC
    TTAACCCCTT CAGTGCCCCC GCCTGCCCCA CCGCTTGACC CCAAATTGAT GAAAGATGCC
    AGGCCTTCTT TCAAGAAACC TGGCCAGCCA GAGCCCTCCC GGGAGGCCTG CAGACAGCCT
    TCCATCAAGG AGGAGGGCAG CAGACTCCCC ATGCCCCTGA TCACCACCGA AGCGTTGCAA
    ATGGTGCAGT TGAGGCCGGT GAAGAAGAAC TCAGGAACCG AGGTAGCCCC CTTACAGGAA
    CACGCATCTC AGGAAAAACG AACTCCGATT GTTTCCCAGT ATCATTTAAA GCCATCTGCT
    TTCCTGAAAT CCCGAAATAG CATACATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCCGCG
    ACGAGCTCGC TCCCGACTCC TGCCAAGAGC TCGACTCAGG GTCACCAGGA CAGTGCAGCC
    GAGCATGGCC TCCAGAGCCA CAGCCCGGCC GGGGAGGCGG AGGCCGGAGC GGGTGCCACC
    CCGCCCCTCG GCCCGTCGCC CAGCAGGAAG CCGCCCCCCA TTTCCAAGAA GCCCAAACTG
    TTCCTCGTGG TGTCACCTCC GCAGAGGGAT TTCACGGCGG AGCCCGCAGA GAACGTGAGC
    GTGGCGTCTC CCAGCCCCAC GAGCGGAGAG GCACGAGAGA GGGGTGCAGC AGGGGCTGGT
    TCCGATGACA CCGACTCTGG CAGCTCGGTG GTTCTTGTGG GAGGAGCTGC AGGATCCGAG
    TCCCCGGGCA GAGTGGAAGC CAATGTCCCC ATGGTGCCAG CCCCTGCGGA GGAGCCGGGT
    GCCAGGAACG GTGTGGAGCG CTGCCTGCCT CCGCAGGACC CAGGGCCTGG GGTGCCGGAG
    GCTGAGACAG CCACTTCTTC TTCAGAGACC TGTGACTTCC CTAAGGACGA CGGGGGTGAT
    GAGGTGATGA CCCCCAGTAG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGG
    TCTAAGAGGA AAGTCCTTGG CCGTAAAGAT TCAGAGGATG ATCACTCCCG AAACCATTCT
    CCCTCCCCAC CAGTGACACC TACTGGTGCC GCCCCCAGCC TGGCCTCCCA GAAGCAAGTG
    GGGTCCATTC AGAGAAGCGT CCGGAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG
    CTGCTGAAAA AGGGGAGCCG CTCGGATACC AGCGCCCGCA TGTCAGCTGC AGAGATGCTC
    AAAAACACAG ACCCTAGATT CCAGAGGTCA AGGTCGGAGC CATCGCCAGA CACGCCCGAG
    AGCCCGTCAA ACTGCTCCCC GAGCAAGAAC AGGAGGGCGC AGGAGGAGTG GGCCAAGACC
    GAAGGCTTGA TGCCTCGGAG TCTGTCCTTC TCCGGCCCCA GGTACGGCCG CAGTCGAACG
    CCGCCTTCTG CCGCCAGCAG CAGGTACAGC GTGCGGAACC GGATCCAGAG CAGCCCCATG
    ACCGTCATCT CGGAGGGCGA AGGGGAAGCC ACGGAGCCCA TAGACAGCAC GGCTCTTCGG
    GCCCCGGGTG CTACAAGGGG GTGTTCGCTG GATGGACTGG CAGGGGGTGG TGTGGATGAG
    GGCAGCCTGC TCTGTGCCGG GGGACCCGCT GCCCCGCTGG AGGCCCAGGC TCCTGGTCCG
    GCAGTGGGGC TGCCCAGCGC AGAGGGCAGG GGCCCTTCAG AACAGCGAGG CGGTCTCGCC
    GGGGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211045.1
    CDS701..5515
    Translation

    Target ORF information:

    RefSeq Version XM_005210988.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210988.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT
    TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA
    GTCCCTGACA GCATACAGAA GGAGCTAGCT TCGGGAGCCA CTGGCCAAGA TGATGTTGGT
    GGTCACTCAG CATACACCCC AGACCACTAC TCTACACTCG GAAGGCTCGA CAGCTATCGA
    TCTATGGGAC AGCGCTCAGA AACCAGGGAT TCCAGCTGTC AGACTGAGGA AGTGAAGGTC
    GTACCACCTT CAATGAGAAG AATCAGAGCC CAGAAGGGGC AAGGCATTGC TGCTCAGATG
    AGTCACTTCT CAGGCTCCTC TGGGAACATG TCAGTGCTGA GTGACTCTGC CGGGGTCGTG
    TTCCCTTCCC GCCTCAACAG CGATGCTGGT TTTCACAGTC TTCCCCGGTC TGGAGCAAGG
    GCAAATGTTC AGGCCCTGGA GCCACGGCTG GGCGCCCTGG GCCCTGCGGA AGACCTGGGT
    GGCGCTTTCC CCTACCAGCA GGGTCACCCA CAAGTAGATG AAGACTTTGG GCATCTAGGA
    GCTGCCCCGA GGATGGGAAC ACTGTTGAGG CCCAAATCTC AGGAGCTGAG GCACTTCGAG
    GGTGAAAATG TAACCAGCCC TGCGTGTGTG GTGTCTCCTC ACACGAGCTA CTCCACCAGC
    ATCATCCCAA ATGCCACGCT CTCCTCCTCT TCAGAGATCA TTGTGATTCA CACTGCTCAG
    AGCTCAGGGC AGCTGGACAG TAAAATTACC AGCTCATCTT CATACTCAAA GGTCAGATCC
    AGAGACCACC TCCTCTCCAG GCAGAAAGAT GGTCATCATT CGTCCAGTGC AACCACACTG
    CTGTCCCTCT GTGACTCCGA AGTCTCTCTA AATACACCAG CAAATCGGGA GAATGGGTCC
    CAGGCCATGG CCTATAACTG TAGAAATAAC CTTAGCATCC CAGCCCACCC CCAGGATGTG
    GACAACAAGA GTGAGTCCAG TTATTCAGGC AGCCGGGGCC AGGGTGGTAG CGTGGAGCCC
    TGGGAATATG GTGGCTCAGG TAGTGGGCGG GCATCCCCAC TGAAGCCACA TTTGGCAGCT
    CCTGGTTACT CCACTCCTGT AAGCAACACG AGCAGCTGCA GTTTGGACCA GACCTCTAAC
    AAGGATGATA CCAGGTCTGT GTATTCAGAG GACCACGATG GCTACTCCCC TACTCTGCAC
    CCGGACTCTG GACACAGACC TGGGGATCTG TGCAATAGTA ATGATGGCTT TGGGAACCCC
    AGGCACAGCG TGGTCAATGT TTTTGATGGA AGAGCTCAGA GACACCAAGG GGACCGGTCC
    AATTGCCAAG ACAAATCCCT CGCGCGAAGC ATTTCTTTGA AGAAAGCCAA GAAGCCTCCC
    CTGCCACCCT CTCGGACGGA CTCTCTGCGC AGGGTTCCCA AGAAGAACGT CCAGTCGAAC
    GGGCAGGTGC TGAACGAGAG CCTGATCGCC TCTCTCCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGATTTCGA AGAACCGTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACTGTG AGCGCCGGCA GCAGCCTAAC CTCCGCCACG
    GCCCCCAACG TCTACTCCGT GTGCGGGCTC ACCCCGGCAC AGAGCGACAC GAGCAGCGTC
    AAGTCCGAGT ACACAGACCC GTGGGGTTAC TACATTGACT ACACGGGAGT ACAGGAAGAT
    CAGGGGAACG CAGGCAGGGC CGGTCCGGCC GGCAGCGCAG CATCACCTGG AAGTGGGCCG
    GGCCGCCATC TCCTGGAGGG GTCCCGGGCT GCAGTACCCC CAGTGCCCGG TGGCGCCGTC
    AAACCAAAGA TCACATCACC GGAGAAGTCG CACAGAGTCA CTTCTCCATC CAGTGGGTAT
    TCCAGTCAGT CGAACACGCC CACCGCTCTC ACCCCTGTGC CTGTGTTCTT AAAATCAATG
    TCACCAGCCA ATGGGAAGGG AAAGCCCAAA CCCAAGGTGC CAGAAAGGAA GTCGTCTCTT
    GTATCTTCAG TATCCGTCTC CTCCTCCTCC ACGTCTCTTT CCTCGAATAC TTCCACTGAA
    GGAAGCGGGA CGGTGAAGAA GCTGGATTCA GCGCTGGTGT CTCCGCTCGC GGCTGCTCCT
    CACACCTCTC CTCCAGCCCC TTGTCCCGCT GACAAGTCTC CTTTCCTCCC TCCCCCGCCA
    CCTCTGGCAG ATTTCTCCGA GGGCTCACCT CTGCCTCGCT CTCCCCTCTT CCCCCCTCCG
    CCACCAGAAG TGCTCACTCC CTTCTGTGCC CCCACCGACG CCTGCTTTCC TCCGCCCCCT
    ACAGCACTGA GCGCCCCTTT TCTGGATTCA TCTGACTCCC TGCCACCTCC GCCACCTGCC
    TTAACCCCTT CAGTGCCCCC GCCTGCCCCA CCGCTTGACC CCAAATTGAT GAAAGATGCC
    AGGCCTTCTT TCAAGAAACC TGGCCAGCCA GAGCCCTCCC GGGAGGCCTG CAGACAGCCT
    TCCATCAAGG AGGAGGGCAG CAGACTCCCC ATGCCCCTGA TCACCACCGA AGCGTTGCAA
    ATGGTGCAGT TGAGGCCGGT GAAGAAGAAC TCAGGAACCG AGGTAGCCCC CTTACAGGAA
    CACGCATCTC AGGAAAAACG AACTCCGATT GTTTCCCAGT ATCATTTAAA GCCATCTGCT
    TTCCTGAAAT CCCGAAATAG CATACATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCCGCG
    ACGAGCTCGC TCCCGACTCC TGCCAAGAGC TCGACTCAGG GTCACCAGGA CAGTGCAGCC
    GAGCATGGCC TCCAGAGCCA CAGCCCGGCC GGGGAGGCGG AGGCCGGAGC GGGTGCCACC
    CCGCCCCTCG GCCCGTCGCC CAGCAGGAAG CCGCCCCCCA TTTCCAAGAA GCCCAAACTG
    TTCCTCGTGG TGTCACCTCC GCAGAGGGAT TTCACGGCGG AGCCCGCAGA GAACGTGAGC
    GTGGCGTCTC CCAGCCCCAC GAGCGGAGAG GCACGAGAGA GGGGTGCAGC AGGGGCTGGT
    TCCGATGACA CCGACTCTGG CAGCTCGGTG GTTCTTGTGG GAGGAGCTGC AGGATCCGAG
    TCCCCGGGCA GAGTGGAAGC CAATGTCCCC ATGGTGCCAG CCCCTGCGGA GGAGCCGGGT
    GCCAGGAACG GTGTGGAGCG CTGCCTGCCT CCGCAGGACC CAGGGCCTGG GGTGCCGGAG
    GCTGAGACAG CCACTTCTTC TTCAGAGACC TGTGACTTCC CTAAGGACGA CGGGGGTGAT
    GAGGTGATGA CCCCCAGTAG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGG
    TCTAAGAGGA AAGTCCTTGG CCGTAAAGAT TCAGAGGATG ATCACTCCCG AAACCATTCT
    CCCTCCCCAC CAGTGACACC TACTGGTGCC GCCCCCAGCC TGGCCTCCCA GAAGCAAGTG
    GGGTCCATTC AGAGAAGCGT CCGGAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG
    CTGCTGAAAA AGGGGAGCCG CTCGGATACC AGCGCCCGCA TGTCAGCTGC AGAGATGCTC
    AAAAACACAG ACCCTAGATT CCAGAGGTCA AGGTCGGAGC CATCGCCAGA CACGCCCGAG
    AGCCCGTCAA ACTGCTCCCC GAGCAAGAAC AGGAGGGCGC AGGAGGAGTG GGCCAAGACC
    GAAGGCTTGA TGCCTCGGAG TCTGTCCTTC TCCGGCCCCA GGTACGGCCG CAGTCGAACG
    CCGCCTTCTG CCGCCAGCAG CAGGTACAGC GTGCGGAACC GGATCCAGAG CAGCCCCATG
    ACCGTCATCT CGGAGGGCGA AGGGGAAGCC ACGGAGCCCA TAGACAGCAC GGCTCTTCGG
    GCCCCGGGTG CTACAAGGGG GTGTTCGCTG GATGGACTGG CAGGGGGTGG TGTGGATGAG
    GGCAGCCTGC TCTGTGCCGG GGGACCCGCT GCCCCGCTGG AGGCCCAGGC TCCTGGTCCG
    GCAGTGGGGC TGCCCAGCGC AGAGGGCAGG GGCCCTTCAG AACAGCGAGG CGGTCTCGCC
    GGGGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22718
    Clone ID Related Accession (Same CDS sequence) XM_005210990.3 , XM_005210990.4
    Accession Version XM_005210990.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGCTTC GGGAGCCACT GGCCAAGATG ATGTTGGTGG TCACTCAGCA
    TACACCCCAG ACCACTACTC TACACTCGGA AGGCTCGACA GCTATCGATC TATGGGACAG
    CGCTCAGAAA CCAGGGATTC CAGCTGTCAG ACTGAGGAAG TGAAGGTCGT ACCACCTTCA
    ATGAGAAGAA TCAGAGCCCA GAAGGGGCAA GGCATTGCTG CTCAGATGAG TCACTTCTCA
    GGCTCCTCTG GGAACATGTC AGTGCTGAGT GACTCTGCCG GGGTCGTGTT CCCTTCCCGC
    CTCAACAGCG ATGCTGGTTT TCACAGTCTT CCCCGGTCTG GAGCAAGGGC AAATGTTCAG
    GCCCTGGAGC CACGGCTGGG CGCCCTGGGC CCTGCGGAAG ACCTGGGTGG CGCTTTCCCC
    TACCAGCAGG GTCACCCACA AGTAGATGAA GACTTTGGGC ATCTAGGAGC TGCCCCGAGG
    ATGGGAACAC TGTTGAGGCC CAAATCTCAG GAGCTGAGGC ACTTCGAGGG TGAAAATGTA
    ACCAGCCCTG CGTGTGTGGT GTCTCCTCAC ACGAGCTACT CCACCAGCAT CATCCCAAAT
    GCCACGCTCT CCTCCTCTTC AGAGATCATT GTGATTCACA CTGCTCAGAG CTCAGGGCAG
    CTGGACAGTA AAATTACCAG CTCATCTTCA TACTCAAAGG TCAGATCCAG AGACCACCTC
    CTCTCCAGGC AGAAAGATGG TCATCATTCG TCCAGTGCAA CCACACTGCT GTCCCTCTGT
    GACTCCGAAG TCTCTCTAAA TACACCAGCA AATCGGGAGA ATGGGTCCCA GGCCATGGCC
    TATAACTGTA GAAATAACCT TAGCATCCCA GCCCACCCCC AGGATGTGGA CAACAAGAGT
    GAGTCCAGTT ATTCAGGCAG CCGGGGCCAG GGTGGTAGCG TGGAGCCCTG GGAATATGGT
    GGCTCAGGTA GTGGGCGGGC ATCCCCACTG AAGCCACATT TGGCAGCTCC TGGTTACTCC
    ACTCCTGTAA GCAACACGAG CAGCTGCAGT TTGGACCAGA CCTCTAACAA GGATGATACC
    AGGTCTGTGT ATTCAGAGGA CCACGATGGC TACTCCCCTA CTCTGCACCC GGACTCTGGA
    CACAGACCTG GGGATCTGTG CAATAGTAAT GATGGCTTTG GGAACCCCAG GCACAGCGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAGA CACCAAGGGG ACCGGTCCAA TTGCCAAGAC
    AAATCCCTCG CGCGAAGCAT TTCTTTGAAG AAAGCCAAGA AGCCTCCCCT GCCACCCTCT
    CGGACGGACT CTCTGCGCAG GGTTCCCAAG AAGAACGTCC AGTCGAACGG GCAGGTGCTG
    AACGAGAGCC TGATCGCCTC TCTCCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GATTTCGAAG AACCGTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACTGTGAG CGCCGGCAGC AGCCTAACCT CCGCCACGGC CCCCAACGTC
    TACTCCGTGT GCGGGCTCAC CCCGGCACAG AGCGACACGA GCAGCGTCAA GTCCGAGTAC
    ACAGACCCGT GGGGTTACTA CATTGACTAC ACGGGAGTAC AGGAAGATCA GGGGAACGCA
    GGCAGGGCCG GTCCGGCCGG CAGCGCAGCA TCACCTGGAA GTGGGCCGGG CCGCCATCTC
    CTGGAGGGGT CCCGGGCTGC AGTACCCCCA GTGCCCGGTG GCGCCGTCAA ACCAAAGATC
    ACATCACCGG AGAAGTCGCA CAGAGTCACT TCTCCATCCA GTGGGTATTC CAGTCAGTCG
    AACACGCCCA CCGCTCTCAC CCCTGTGCCT GTGTTCTTAA AATCAATGTC ACCAGCCAAT
    GGGAAGGGAA AGCCCAAACC CAAGGTGCCA GAAAGGAAGT CGTCTCTTGT ATCTTCAGTA
    TCCGTCTCCT CCTCCTCCAC GTCTCTTTCC TCGAATACTT CCACTGAAGG AAGCGGGACG
    GTGAAGAAGC TGGATTCAGC GCTGGTGTCT CCGCTCGCGG CTGCTCCTCA CACCTCTCCT
    CCAGCCCCTT GTCCCGCTGA CAAGTCTCCT TTCCTCCCTC CCCCGCCACC TCTGGCAGAT
    TTCTCCGAGG GCTCACCTCT GCCTCGCTCT CCCCTCTTCC CCCCTCCGCC ACCAGAAGTG
    CTCACTCCCT TCTGTGCCCC CACCGACGCC TGCTTTCCTC CGCCCCCTAC AGCACTGAGC
    GCCCCTTTTC TGGATTCATC TGACTCCCTG CCACCTCCGC CACCTGCCTT AACCCCTTCA
    GTGCCCCCGC CTGCCCCACC GCTTGACCCC AAATTGATGA AAGATGCCAG GCCTTCTTTC
    AAGAAACCTG GCCAGCCAGA GCCCTCCCGG GAGGCCTGCA GACAGCCTTC CATCAAGGAG
    GAGGGCAGCA GACTCCCCAT GCCCCTGATC ACCACCGAAG CGTTGCAAAT GGTGCAGTTG
    AGGCCGGTGA AGAAGAACTC AGGAACCGAG GTAGCCCCCT TACAGGAACA CGCATCTCAG
    GAAAAACGAA CTCCGATTGT TTCCCAGTAT CATTTAAAGC CATCTGCTTT CCTGAAATCC
    CGAAATAGCA TACATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCCGCGAC GAGCTCGCTC
    CCGACTCCTG CCAAGAGCTC GACTCAGGGT CACCAGGACA GTGCAGCCGA GCATGGCCTC
    CAGAGCCACA GCCCGGCCGG GGAGGCGGAG GCCGGAGCGG GTGCCACCCC GCCCCTCGGC
    CCGTCGCCCA GCAGGAAGCC GCCCCCCATT TCCAAGAAGC CCAAACTGTT CCTCGTGGTG
    TCACCTCCGC AGAGGGATTT CACGGCGGAG CCCGCAGAGA ACGTGAGCGT GGCGTCTCCC
    AGCCCCACGA GCGGAGAGGC ACGAGAGAGG GGTGCAGCAG GGGCTGGTTC CGATGACACC
    GACTCTGGCA GCTCGGTGGT TCTTGTGGGA GGAGCTGCAG GATCCGAGTC CCCGGGCAGA
    GTGGAAGCCA ATGTCCCCAT GGTGCCAGCC CCTGCGGAGG AGCCGGGTGC CAGGAACGGT
    GTGGAGCGCT GCCTGCCTCC GCAGGACCCA GGGCCTGGGG TGCCGGAGGC TGAGACAGCC
    ACTTCTTCTT CAGAGACCTG TGACTTCCCT AAGGACGACG GGGGTGATGA GGTGATGACC
    CCCAGTAGAC CCAGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGGTC TAAGAGGAAA
    GTCCTTGGCC GTAAAGATTC AGAGGATGAT CACTCCCGAA ACCATTCTCC CTCCCCACCA
    GTGACACCTA CTGGTGCCGC CCCCAGCCTG GCCTCCCAGA AGCAAGTGGG GTCCATTCAG
    AGAAGCGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTGAAAAAG
    GGGAGCCGCT CGGATACCAG CGCCCGCATG TCAGCTGCAG AGATGCTCAA AAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCGGAGCCA TCGCCAGACA CGCCCGAGAG CCCGTCAAAC
    TGCTCCCCGA GCAAGAACAG GAGGGCGCAG GAGGAGTGGG CCAAGACCGA AGGCTTGATG
    CCTCGGAGTC TGTCCTTCTC CGGCCCCAGG TACGGCCGCA GTCGAACGCC GCCTTCTGCC
    GCCAGCAGCA GGTACAGCGT GCGGAACCGG ATCCAGAGCA GCCCCATGAC CGTCATCTCG
    GAGGGCGAAG GGGAAGCCAC GGAGCCCATA GACAGCACGG CTCTTCGGGC CCCGGGTGCT
    ACAAGGGGGT GTTCGCTGGA TGGACTGGCA GGGGGTGGTG TGGATGAGGG CAGCCTGCTC
    TGTGCCGGGG GACCCGCTGC CCCGCTGGAG GCCCAGGCTC CTGGTCCGGC AGTGGGGCTG
    CCCAGCGCAG AGGGCAGGGG CCCTTCAGAA CAGCGAGGCG GTCTCGCCGG GGATAAGAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211047.1
    CDS701..5383
    Translation

    Target ORF information:

    RefSeq Version XM_005210990.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210990.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGCTTC GGGAGCCACT GGCCAAGATG ATGTTGGTGG TCACTCAGCA
    TACACCCCAG ACCACTACTC TACACTCGGA AGGCTCGACA GCTATCGATC TATGGGACAG
    CGCTCAGAAA CCAGGGATTC CAGCTGTCAG ACTGAGGAAG TGAAGGTCGT ACCACCTTCA
    ATGAGAAGAA TCAGAGCCCA GAAGGGGCAA GGCATTGCTG CTCAGATGAG TCACTTCTCA
    GGCTCCTCTG GGAACATGTC AGTGCTGAGT GACTCTGCCG GGGTCGTGTT CCCTTCCCGC
    CTCAACAGCG ATGCTGGTTT TCACAGTCTT CCCCGGTCTG GAGCAAGGGC AAATGTTCAG
    GCCCTGGAGC CACGGCTGGG CGCCCTGGGC CCTGCGGAAG ACCTGGGTGG CGCTTTCCCC
    TACCAGCAGG GTCACCCACA AGTAGATGAA GACTTTGGGC ATCTAGGAGC TGCCCCGAGG
    ATGGGAACAC TGTTGAGGCC CAAATCTCAG GAGCTGAGGC ACTTCGAGGG TGAAAATGTA
    ACCAGCCCTG CGTGTGTGGT GTCTCCTCAC ACGAGCTACT CCACCAGCAT CATCCCAAAT
    GCCACGCTCT CCTCCTCTTC AGAGATCATT GTGATTCACA CTGCTCAGAG CTCAGGGCAG
    CTGGACAGTA AAATTACCAG CTCATCTTCA TACTCAAAGG TCAGATCCAG AGACCACCTC
    CTCTCCAGGC AGAAAGATGG TCATCATTCG TCCAGTGCAA CCACACTGCT GTCCCTCTGT
    GACTCCGAAG TCTCTCTAAA TACACCAGCA AATCGGGAGA ATGGGTCCCA GGCCATGGCC
    TATAACTGTA GAAATAACCT TAGCATCCCA GCCCACCCCC AGGATGTGGA CAACAAGAGT
    GAGTCCAGTT ATTCAGGCAG CCGGGGCCAG GGTGGTAGCG TGGAGCCCTG GGAATATGGT
    GGCTCAGGTA GTGGGCGGGC ATCCCCACTG AAGCCACATT TGGCAGCTCC TGGTTACTCC
    ACTCCTGTAA GCAACACGAG CAGCTGCAGT TTGGACCAGA CCTCTAACAA GGATGATACC
    AGGTCTGTGT ATTCAGAGGA CCACGATGGC TACTCCCCTA CTCTGCACCC GGACTCTGGA
    CACAGACCTG GGGATCTGTG CAATAGTAAT GATGGCTTTG GGAACCCCAG GCACAGCGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAGA CACCAAGGGG ACCGGTCCAA TTGCCAAGAC
    AAATCCCTCG CGCGAAGCAT TTCTTTGAAG AAAGCCAAGA AGCCTCCCCT GCCACCCTCT
    CGGACGGACT CTCTGCGCAG GGTTCCCAAG AAGAACGTCC AGTCGAACGG GCAGGTGCTG
    AACGAGAGCC TGATCGCCTC TCTCCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GATTTCGAAG AACCGTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACTGTGAG CGCCGGCAGC AGCCTAACCT CCGCCACGGC CCCCAACGTC
    TACTCCGTGT GCGGGCTCAC CCCGGCACAG AGCGACACGA GCAGCGTCAA GTCCGAGTAC
    ACAGACCCGT GGGGTTACTA CATTGACTAC ACGGGAGTAC AGGAAGATCA GGGGAACGCA
    GGCAGGGCCG GTCCGGCCGG CAGCGCAGCA TCACCTGGAA GTGGGCCGGG CCGCCATCTC
    CTGGAGGGGT CCCGGGCTGC AGTACCCCCA GTGCCCGGTG GCGCCGTCAA ACCAAAGATC
    ACATCACCGG AGAAGTCGCA CAGAGTCACT TCTCCATCCA GTGGGTATTC CAGTCAGTCG
    AACACGCCCA CCGCTCTCAC CCCTGTGCCT GTGTTCTTAA AATCAATGTC ACCAGCCAAT
    GGGAAGGGAA AGCCCAAACC CAAGGTGCCA GAAAGGAAGT CGTCTCTTGT ATCTTCAGTA
    TCCGTCTCCT CCTCCTCCAC GTCTCTTTCC TCGAATACTT CCACTGAAGG AAGCGGGACG
    GTGAAGAAGC TGGATTCAGC GCTGGTGTCT CCGCTCGCGG CTGCTCCTCA CACCTCTCCT
    CCAGCCCCTT GTCCCGCTGA CAAGTCTCCT TTCCTCCCTC CCCCGCCACC TCTGGCAGAT
    TTCTCCGAGG GCTCACCTCT GCCTCGCTCT CCCCTCTTCC CCCCTCCGCC ACCAGAAGTG
    CTCACTCCCT TCTGTGCCCC CACCGACGCC TGCTTTCCTC CGCCCCCTAC AGCACTGAGC
    GCCCCTTTTC TGGATTCATC TGACTCCCTG CCACCTCCGC CACCTGCCTT AACCCCTTCA
    GTGCCCCCGC CTGCCCCACC GCTTGACCCC AAATTGATGA AAGATGCCAG GCCTTCTTTC
    AAGAAACCTG GCCAGCCAGA GCCCTCCCGG GAGGCCTGCA GACAGCCTTC CATCAAGGAG
    GAGGGCAGCA GACTCCCCAT GCCCCTGATC ACCACCGAAG CGTTGCAAAT GGTGCAGTTG
    AGGCCGGTGA AGAAGAACTC AGGAACCGAG GTAGCCCCCT TACAGGAACA CGCATCTCAG
    GAAAAACGAA CTCCGATTGT TTCCCAGTAT CATTTAAAGC CATCTGCTTT CCTGAAATCC
    CGAAATAGCA TACATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCCGCGAC GAGCTCGCTC
    CCGACTCCTG CCAAGAGCTC GACTCAGGGT CACCAGGACA GTGCAGCCGA GCATGGCCTC
    CAGAGCCACA GCCCGGCCGG GGAGGCGGAG GCCGGAGCGG GTGCCACCCC GCCCCTCGGC
    CCGTCGCCCA GCAGGAAGCC GCCCCCCATT TCCAAGAAGC CCAAACTGTT CCTCGTGGTG
    TCACCTCCGC AGAGGGATTT CACGGCGGAG CCCGCAGAGA ACGTGAGCGT GGCGTCTCCC
    AGCCCCACGA GCGGAGAGGC ACGAGAGAGG GGTGCAGCAG GGGCTGGTTC CGATGACACC
    GACTCTGGCA GCTCGGTGGT TCTTGTGGGA GGAGCTGCAG GATCCGAGTC CCCGGGCAGA
    GTGGAAGCCA ATGTCCCCAT GGTGCCAGCC CCTGCGGAGG AGCCGGGTGC CAGGAACGGT
    GTGGAGCGCT GCCTGCCTCC GCAGGACCCA GGGCCTGGGG TGCCGGAGGC TGAGACAGCC
    ACTTCTTCTT CAGAGACCTG TGACTTCCCT AAGGACGACG GGGGTGATGA GGTGATGACC
    CCCAGTAGAC CCAGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGGTC TAAGAGGAAA
    GTCCTTGGCC GTAAAGATTC AGAGGATGAT CACTCCCGAA ACCATTCTCC CTCCCCACCA
    GTGACACCTA CTGGTGCCGC CCCCAGCCTG GCCTCCCAGA AGCAAGTGGG GTCCATTCAG
    AGAAGCGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTGAAAAAG
    GGGAGCCGCT CGGATACCAG CGCCCGCATG TCAGCTGCAG AGATGCTCAA AAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCGGAGCCA TCGCCAGACA CGCCCGAGAG CCCGTCAAAC
    TGCTCCCCGA GCAAGAACAG GAGGGCGCAG GAGGAGTGGG CCAAGACCGA AGGCTTGATG
    CCTCGGAGTC TGTCCTTCTC CGGCCCCAGG TACGGCCGCA GTCGAACGCC GCCTTCTGCC
    GCCAGCAGCA GGTACAGCGT GCGGAACCGG ATCCAGAGCA GCCCCATGAC CGTCATCTCG
    GAGGGCGAAG GGGAAGCCAC GGAGCCCATA GACAGCACGG CTCTTCGGGC CCCGGGTGCT
    ACAAGGGGGT GTTCGCTGGA TGGACTGGCA GGGGGTGGTG TGGATGAGGG CAGCCTGCTC
    TGTGCCGGGG GACCCGCTGC CCCGCTGGAG GCCCAGGCTC CTGGTCCGGC AGTGGGGCTG
    CCCAGCGCAG AGGGCAGGGG CCCTTCAGAA CAGCGAGGCG GTCTCGCCGG GGATAAGAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22718
    Clone ID Related Accession (Same CDS sequence) XM_005210990.3 , XM_005210990.4
    Accession Version XM_005210990.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGCTTC GGGAGCCACT GGCCAAGATG ATGTTGGTGG TCACTCAGCA
    TACACCCCAG ACCACTACTC TACACTCGGA AGGCTCGACA GCTATCGATC TATGGGACAG
    CGCTCAGAAA CCAGGGATTC CAGCTGTCAG ACTGAGGAAG TGAAGGTCGT ACCACCTTCA
    ATGAGAAGAA TCAGAGCCCA GAAGGGGCAA GGCATTGCTG CTCAGATGAG TCACTTCTCA
    GGCTCCTCTG GGAACATGTC AGTGCTGAGT GACTCTGCCG GGGTCGTGTT CCCTTCCCGC
    CTCAACAGCG ATGCTGGTTT TCACAGTCTT CCCCGGTCTG GAGCAAGGGC AAATGTTCAG
    GCCCTGGAGC CACGGCTGGG CGCCCTGGGC CCTGCGGAAG ACCTGGGTGG CGCTTTCCCC
    TACCAGCAGG GTCACCCACA AGTAGATGAA GACTTTGGGC ATCTAGGAGC TGCCCCGAGG
    ATGGGAACAC TGTTGAGGCC CAAATCTCAG GAGCTGAGGC ACTTCGAGGG TGAAAATGTA
    ACCAGCCCTG CGTGTGTGGT GTCTCCTCAC ACGAGCTACT CCACCAGCAT CATCCCAAAT
    GCCACGCTCT CCTCCTCTTC AGAGATCATT GTGATTCACA CTGCTCAGAG CTCAGGGCAG
    CTGGACAGTA AAATTACCAG CTCATCTTCA TACTCAAAGG TCAGATCCAG AGACCACCTC
    CTCTCCAGGC AGAAAGATGG TCATCATTCG TCCAGTGCAA CCACACTGCT GTCCCTCTGT
    GACTCCGAAG TCTCTCTAAA TACACCAGCA AATCGGGAGA ATGGGTCCCA GGCCATGGCC
    TATAACTGTA GAAATAACCT TAGCATCCCA GCCCACCCCC AGGATGTGGA CAACAAGAGT
    GAGTCCAGTT ATTCAGGCAG CCGGGGCCAG GGTGGTAGCG TGGAGCCCTG GGAATATGGT
    GGCTCAGGTA GTGGGCGGGC ATCCCCACTG AAGCCACATT TGGCAGCTCC TGGTTACTCC
    ACTCCTGTAA GCAACACGAG CAGCTGCAGT TTGGACCAGA CCTCTAACAA GGATGATACC
    AGGTCTGTGT ATTCAGAGGA CCACGATGGC TACTCCCCTA CTCTGCACCC GGACTCTGGA
    CACAGACCTG GGGATCTGTG CAATAGTAAT GATGGCTTTG GGAACCCCAG GCACAGCGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAGA CACCAAGGGG ACCGGTCCAA TTGCCAAGAC
    AAATCCCTCG CGCGAAGCAT TTCTTTGAAG AAAGCCAAGA AGCCTCCCCT GCCACCCTCT
    CGGACGGACT CTCTGCGCAG GGTTCCCAAG AAGAACGTCC AGTCGAACGG GCAGGTGCTG
    AACGAGAGCC TGATCGCCTC TCTCCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GATTTCGAAG AACCGTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACTGTGAG CGCCGGCAGC AGCCTAACCT CCGCCACGGC CCCCAACGTC
    TACTCCGTGT GCGGGCTCAC CCCGGCACAG AGCGACACGA GCAGCGTCAA GTCCGAGTAC
    ACAGACCCGT GGGGTTACTA CATTGACTAC ACGGGAGTAC AGGAAGATCA GGGGAACGCA
    GGCAGGGCCG GTCCGGCCGG CAGCGCAGCA TCACCTGGAA GTGGGCCGGG CCGCCATCTC
    CTGGAGGGGT CCCGGGCTGC AGTACCCCCA GTGCCCGGTG GCGCCGTCAA ACCAAAGATC
    ACATCACCGG AGAAGTCGCA CAGAGTCACT TCTCCATCCA GTGGGTATTC CAGTCAGTCG
    AACACGCCCA CCGCTCTCAC CCCTGTGCCT GTGTTCTTAA AATCAATGTC ACCAGCCAAT
    GGGAAGGGAA AGCCCAAACC CAAGGTGCCA GAAAGGAAGT CGTCTCTTGT ATCTTCAGTA
    TCCGTCTCCT CCTCCTCCAC GTCTCTTTCC TCGAATACTT CCACTGAAGG AAGCGGGACG
    GTGAAGAAGC TGGATTCAGC GCTGGTGTCT CCGCTCGCGG CTGCTCCTCA CACCTCTCCT
    CCAGCCCCTT GTCCCGCTGA CAAGTCTCCT TTCCTCCCTC CCCCGCCACC TCTGGCAGAT
    TTCTCCGAGG GCTCACCTCT GCCTCGCTCT CCCCTCTTCC CCCCTCCGCC ACCAGAAGTG
    CTCACTCCCT TCTGTGCCCC CACCGACGCC TGCTTTCCTC CGCCCCCTAC AGCACTGAGC
    GCCCCTTTTC TGGATTCATC TGACTCCCTG CCACCTCCGC CACCTGCCTT AACCCCTTCA
    GTGCCCCCGC CTGCCCCACC GCTTGACCCC AAATTGATGA AAGATGCCAG GCCTTCTTTC
    AAGAAACCTG GCCAGCCAGA GCCCTCCCGG GAGGCCTGCA GACAGCCTTC CATCAAGGAG
    GAGGGCAGCA GACTCCCCAT GCCCCTGATC ACCACCGAAG CGTTGCAAAT GGTGCAGTTG
    AGGCCGGTGA AGAAGAACTC AGGAACCGAG GTAGCCCCCT TACAGGAACA CGCATCTCAG
    GAAAAACGAA CTCCGATTGT TTCCCAGTAT CATTTAAAGC CATCTGCTTT CCTGAAATCC
    CGAAATAGCA TACATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCCGCGAC GAGCTCGCTC
    CCGACTCCTG CCAAGAGCTC GACTCAGGGT CACCAGGACA GTGCAGCCGA GCATGGCCTC
    CAGAGCCACA GCCCGGCCGG GGAGGCGGAG GCCGGAGCGG GTGCCACCCC GCCCCTCGGC
    CCGTCGCCCA GCAGGAAGCC GCCCCCCATT TCCAAGAAGC CCAAACTGTT CCTCGTGGTG
    TCACCTCCGC AGAGGGATTT CACGGCGGAG CCCGCAGAGA ACGTGAGCGT GGCGTCTCCC
    AGCCCCACGA GCGGAGAGGC ACGAGAGAGG GGTGCAGCAG GGGCTGGTTC CGATGACACC
    GACTCTGGCA GCTCGGTGGT TCTTGTGGGA GGAGCTGCAG GATCCGAGTC CCCGGGCAGA
    GTGGAAGCCA ATGTCCCCAT GGTGCCAGCC CCTGCGGAGG AGCCGGGTGC CAGGAACGGT
    GTGGAGCGCT GCCTGCCTCC GCAGGACCCA GGGCCTGGGG TGCCGGAGGC TGAGACAGCC
    ACTTCTTCTT CAGAGACCTG TGACTTCCCT AAGGACGACG GGGGTGATGA GGTGATGACC
    CCCAGTAGAC CCAGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGGTC TAAGAGGAAA
    GTCCTTGGCC GTAAAGATTC AGAGGATGAT CACTCCCGAA ACCATTCTCC CTCCCCACCA
    GTGACACCTA CTGGTGCCGC CCCCAGCCTG GCCTCCCAGA AGCAAGTGGG GTCCATTCAG
    AGAAGCGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTGAAAAAG
    GGGAGCCGCT CGGATACCAG CGCCCGCATG TCAGCTGCAG AGATGCTCAA AAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCGGAGCCA TCGCCAGACA CGCCCGAGAG CCCGTCAAAC
    TGCTCCCCGA GCAAGAACAG GAGGGCGCAG GAGGAGTGGG CCAAGACCGA AGGCTTGATG
    CCTCGGAGTC TGTCCTTCTC CGGCCCCAGG TACGGCCGCA GTCGAACGCC GCCTTCTGCC
    GCCAGCAGCA GGTACAGCGT GCGGAACCGG ATCCAGAGCA GCCCCATGAC CGTCATCTCG
    GAGGGCGAAG GGGAAGCCAC GGAGCCCATA GACAGCACGG CTCTTCGGGC CCCGGGTGCT
    ACAAGGGGGT GTTCGCTGGA TGGACTGGCA GGGGGTGGTG TGGATGAGGG CAGCCTGCTC
    TGTGCCGGGG GACCCGCTGC CCCGCTGGAG GCCCAGGCTC CTGGTCCGGC AGTGGGGCTG
    CCCAGCGCAG AGGGCAGGGG CCCTTCAGAA CAGCGAGGCG GTCTCGCCGG GGATAAGAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211047.1
    CDS1501..6183
    Translation

    Target ORF information:

    RefSeq Version XM_005210990.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210990.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCTTC AGGTTCCTTT AGACTCAAGT CAAACCCAGG TTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AAACAAAGAG GCTGTTTCGC
    AGTGATGGGG AGTTGTCGGT CTGTGGGCAG CAGGTGGAAG CAGATGATGA AAACTGGACC
    TACAGAACCC AGCCCAGAAA AGCTGTTTCC AACCTAGATG AGGAGAGTCG ATGGACGGTC
    CACTACACTG CTCCATGGCA CCAGCAGGAG AATGTGTTCC TCCCCACCAC AAGACCCCCC
    TGCGTGGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGT
    GATAAGTTAC GGCGGGATGG CTATCGGAGT TCTCAGTACT ATTCCCAAGG ACCCACCTTT
    GCAGCCAACT CCAGTCCCCT CTGTGATGAT TACCAAGATG AGGATGAAGA AACAGATCCA
    AAGTGTTCTG TCTCTTCATC AGAAGAGGAA AGACTTACTT CCACCAGGAG ACCTAAGACG
    CCAACGTCAA GTGACTGCTC TGACCTTCAT ACTCAAACCA ACTGGACCAA GTCACTTCCC
    TTGCCAACAC CGGAAGAGAA GATGCGGCAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTT
    CCTATTAACA TAACTGCTTC GGGAGCCACT GGCCAAGATG ATGTTGGTGG TCACTCAGCA
    TACACCCCAG ACCACTACTC TACACTCGGA AGGCTCGACA GCTATCGATC TATGGGACAG
    CGCTCAGAAA CCAGGGATTC CAGCTGTCAG ACTGAGGAAG TGAAGGTCGT ACCACCTTCA
    ATGAGAAGAA TCAGAGCCCA GAAGGGGCAA GGCATTGCTG CTCAGATGAG TCACTTCTCA
    GGCTCCTCTG GGAACATGTC AGTGCTGAGT GACTCTGCCG GGGTCGTGTT CCCTTCCCGC
    CTCAACAGCG ATGCTGGTTT TCACAGTCTT CCCCGGTCTG GAGCAAGGGC AAATGTTCAG
    GCCCTGGAGC CACGGCTGGG CGCCCTGGGC CCTGCGGAAG ACCTGGGTGG CGCTTTCCCC
    TACCAGCAGG GTCACCCACA AGTAGATGAA GACTTTGGGC ATCTAGGAGC TGCCCCGAGG
    ATGGGAACAC TGTTGAGGCC CAAATCTCAG GAGCTGAGGC ACTTCGAGGG TGAAAATGTA
    ACCAGCCCTG CGTGTGTGGT GTCTCCTCAC ACGAGCTACT CCACCAGCAT CATCCCAAAT
    GCCACGCTCT CCTCCTCTTC AGAGATCATT GTGATTCACA CTGCTCAGAG CTCAGGGCAG
    CTGGACAGTA AAATTACCAG CTCATCTTCA TACTCAAAGG TCAGATCCAG AGACCACCTC
    CTCTCCAGGC AGAAAGATGG TCATCATTCG TCCAGTGCAA CCACACTGCT GTCCCTCTGT
    GACTCCGAAG TCTCTCTAAA TACACCAGCA AATCGGGAGA ATGGGTCCCA GGCCATGGCC
    TATAACTGTA GAAATAACCT TAGCATCCCA GCCCACCCCC AGGATGTGGA CAACAAGAGT
    GAGTCCAGTT ATTCAGGCAG CCGGGGCCAG GGTGGTAGCG TGGAGCCCTG GGAATATGGT
    GGCTCAGGTA GTGGGCGGGC ATCCCCACTG AAGCCACATT TGGCAGCTCC TGGTTACTCC
    ACTCCTGTAA GCAACACGAG CAGCTGCAGT TTGGACCAGA CCTCTAACAA GGATGATACC
    AGGTCTGTGT ATTCAGAGGA CCACGATGGC TACTCCCCTA CTCTGCACCC GGACTCTGGA
    CACAGACCTG GGGATCTGTG CAATAGTAAT GATGGCTTTG GGAACCCCAG GCACAGCGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAGA CACCAAGGGG ACCGGTCCAA TTGCCAAGAC
    AAATCCCTCG CGCGAAGCAT TTCTTTGAAG AAAGCCAAGA AGCCTCCCCT GCCACCCTCT
    CGGACGGACT CTCTGCGCAG GGTTCCCAAG AAGAACGTCC AGTCGAACGG GCAGGTGCTG
    AACGAGAGCC TGATCGCCTC TCTCCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GATTTCGAAG AACCGTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACTGTGAG CGCCGGCAGC AGCCTAACCT CCGCCACGGC CCCCAACGTC
    TACTCCGTGT GCGGGCTCAC CCCGGCACAG AGCGACACGA GCAGCGTCAA GTCCGAGTAC
    ACAGACCCGT GGGGTTACTA CATTGACTAC ACGGGAGTAC AGGAAGATCA GGGGAACGCA
    GGCAGGGCCG GTCCGGCCGG CAGCGCAGCA TCACCTGGAA GTGGGCCGGG CCGCCATCTC
    CTGGAGGGGT CCCGGGCTGC AGTACCCCCA GTGCCCGGTG GCGCCGTCAA ACCAAAGATC
    ACATCACCGG AGAAGTCGCA CAGAGTCACT TCTCCATCCA GTGGGTATTC CAGTCAGTCG
    AACACGCCCA CCGCTCTCAC CCCTGTGCCT GTGTTCTTAA AATCAATGTC ACCAGCCAAT
    GGGAAGGGAA AGCCCAAACC CAAGGTGCCA GAAAGGAAGT CGTCTCTTGT ATCTTCAGTA
    TCCGTCTCCT CCTCCTCCAC GTCTCTTTCC TCGAATACTT CCACTGAAGG AAGCGGGACG
    GTGAAGAAGC TGGATTCAGC GCTGGTGTCT CCGCTCGCGG CTGCTCCTCA CACCTCTCCT
    CCAGCCCCTT GTCCCGCTGA CAAGTCTCCT TTCCTCCCTC CCCCGCCACC TCTGGCAGAT
    TTCTCCGAGG GCTCACCTCT GCCTCGCTCT CCCCTCTTCC CCCCTCCGCC ACCAGAAGTG
    CTCACTCCCT TCTGTGCCCC CACCGACGCC TGCTTTCCTC CGCCCCCTAC AGCACTGAGC
    GCCCCTTTTC TGGATTCATC TGACTCCCTG CCACCTCCGC CACCTGCCTT AACCCCTTCA
    GTGCCCCCGC CTGCCCCACC GCTTGACCCC AAATTGATGA AAGATGCCAG GCCTTCTTTC
    AAGAAACCTG GCCAGCCAGA GCCCTCCCGG GAGGCCTGCA GACAGCCTTC CATCAAGGAG
    GAGGGCAGCA GACTCCCCAT GCCCCTGATC ACCACCGAAG CGTTGCAAAT GGTGCAGTTG
    AGGCCGGTGA AGAAGAACTC AGGAACCGAG GTAGCCCCCT TACAGGAACA CGCATCTCAG
    GAAAAACGAA CTCCGATTGT TTCCCAGTAT CATTTAAAGC CATCTGCTTT CCTGAAATCC
    CGAAATAGCA TACATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCCGCGAC GAGCTCGCTC
    CCGACTCCTG CCAAGAGCTC GACTCAGGGT CACCAGGACA GTGCAGCCGA GCATGGCCTC
    CAGAGCCACA GCCCGGCCGG GGAGGCGGAG GCCGGAGCGG GTGCCACCCC GCCCCTCGGC
    CCGTCGCCCA GCAGGAAGCC GCCCCCCATT TCCAAGAAGC CCAAACTGTT CCTCGTGGTG
    TCACCTCCGC AGAGGGATTT CACGGCGGAG CCCGCAGAGA ACGTGAGCGT GGCGTCTCCC
    AGCCCCACGA GCGGAGAGGC ACGAGAGAGG GGTGCAGCAG GGGCTGGTTC CGATGACACC
    GACTCTGGCA GCTCGGTGGT TCTTGTGGGA GGAGCTGCAG GATCCGAGTC CCCGGGCAGA
    GTGGAAGCCA ATGTCCCCAT GGTGCCAGCC CCTGCGGAGG AGCCGGGTGC CAGGAACGGT
    GTGGAGCGCT GCCTGCCTCC GCAGGACCCA GGGCCTGGGG TGCCGGAGGC TGAGACAGCC
    ACTTCTTCTT CAGAGACCTG TGACTTCCCT AAGGACGACG GGGGTGATGA GGTGATGACC
    CCCAGTAGAC CCAGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGGTC TAAGAGGAAA
    GTCCTTGGCC GTAAAGATTC AGAGGATGAT CACTCCCGAA ACCATTCTCC CTCCCCACCA
    GTGACACCTA CTGGTGCCGC CCCCAGCCTG GCCTCCCAGA AGCAAGTGGG GTCCATTCAG
    AGAAGCGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTGAAAAAG
    GGGAGCCGCT CGGATACCAG CGCCCGCATG TCAGCTGCAG AGATGCTCAA AAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCGGAGCCA TCGCCAGACA CGCCCGAGAG CCCGTCAAAC
    TGCTCCCCGA GCAAGAACAG GAGGGCGCAG GAGGAGTGGG CCAAGACCGA AGGCTTGATG
    CCTCGGAGTC TGTCCTTCTC CGGCCCCAGG TACGGCCGCA GTCGAACGCC GCCTTCTGCC
    GCCAGCAGCA GGTACAGCGT GCGGAACCGG ATCCAGAGCA GCCCCATGAC CGTCATCTCG
    GAGGGCGAAG GGGAAGCCAC GGAGCCCATA GACAGCACGG CTCTTCGGGC CCCGGGTGCT
    ACAAGGGGGT GTTCGCTGGA TGGACTGGCA GGGGGTGGTG TGGATGAGGG CAGCCTGCTC
    TGTGCCGGGG GACCCGCTGC CCCGCTGGAG GCCCAGGCTC CTGGTCCGGC AGTGGGGCTG
    CCCAGCGCAG AGGGCAGGGG CCCTTCAGAA CAGCGAGGCG GTCTCGCCGG GGATAAGAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22719
    Clone ID Related Accession (Same CDS sequence) XM_024996597.1
    Accession Version XM_024996597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCTGCC TGAAAGCTGT TTCCAACCTA GATGAGGAGA GTCGATGGAC GGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTCCCCA CCACAAGACC CCCCTGCGTG
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGA ATGTGATAAG
    TTACGGCGGG ATGGCTATCG GAGTTCTCAG TACTATTCCC AAGGACCCAC CTTTGCAGCC
    AACTCCAGTC CCCTCTGTGA TGATTACCAA GATGAGGATG AAGAAACAGA TCCAAAGTGT
    TCTGTCTCTT CATCAGAAGA GGAAAGACTT ACTTCCACCA GGAGACCTAA GACGCCAACG
    TCAAGTGACT GCTCTGACCT TCATACTCAA ACCAACTGGA CCAAGTCACT TCCCTTGCCA
    ACACCGGAAG AGAAGATGCG GCAGCAAGCC CAAACAGTCC AGGCTGACGT GGTTCCTATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAGCATAC AGAAGGAGCT AGCTTCGGGA GCCACTGGCC AAGATGATGT TGGTGGTCAC
    TCAGCATACA CCCCAGACCA CTACTCTACA CTCGGAAGGC TCGACAGCTA TCGATCTATG
    GGACAGCGCT CAGAAACCAG GGATTCCAGC TGTCAGACTG AGGAAGTGAA GGTCGTACCA
    CCTTCAATGA GAAGAATCAG AGCCCAGAAG GGGCAAGGCA TTGCTGCTCA GATGAGTCAC
    TTCTCAGGCT CCTCTGGGAA CATGTCAGTG CTGAGTGACT CTGCCGGGGT CGTGTTCCCT
    TCCCGCCTCA ACAGCGATGC TGGTTTTCAC AGTCTTCCCC GGTCTGGAGC AAGGGCAAAT
    GTTCAGGCCC TGGAGCCACG GCTGGGCGCC CTGGGCCCTG CGGAAGACCT GGGTGGCGCT
    TTCCCCTACC AGCAGGGTCA CCCACAAGTA GATGAAGACT TTGGGCATCT AGGAGCTGCC
    CCGAGGATGG GAACACTGTT GAGGCCCAAA TCTCAGGAGC TGAGGCACTT CGAGGGTGAA
    AATGTAACCA GCCCTGCGTG TGTGGTGTCT CCTCACACGA GCTACTCCAC CAGCATCATC
    CCAAATGCCA CGCTCTCCTC CTCTTCAGAG ATCATTGTGA TTCACACTGC TCAGAGCTCA
    GGGCAGCTGG ACAGTAAAAT TACCAGCTCA TCTTCATACT CAAAGGTCAG ATCCAGAGAC
    CACCTCCTCT CCAGGCAGAA AGATGGTCAT CATTCGTCCA GTGCAACCAC ACTGCTGTCC
    CTCTGTGACT CCGAAGTCTC TCTAAATACA CCAGCAAATC GGGAGAATGG GTCCCAGGCC
    ATGGCCTATA ACTGTAGAAA TAACCTTAGC ATCCCAGCCC ACCCCCAGGA TGTGGACAAC
    AAGAGTGAGT CCAGTTATTC AGGCAGCCGG GGCCAGGGTG GTAGCGTGGA GCCCTGGGAA
    TATGGTGGCT CAGGTAGTGG GCGGGCATCC CCACTGAAGC CACATTTGGC AGCTCCTGGT
    TACTCCACTC CTGTAAGCAA CACGAGCAGC TGCAGTTTGG ACCAGACCTC TAACAAGGAT
    GATACCAGGT CTGTGTATTC AGAGGACCAC GATGGCTACT CCCCTACTCT GCACCCGGAC
    TCTGGACACA GACCTGGGGA TCTGTGCAAT AGTAATGATG GCTTTGGGAA CCCCAGGCAC
    AGCGTGGTCA ATGTTTTTGA TGGAAGAGCT CAGAGACACC AAGGGGACCG GTCCAATTGC
    CAAGACAAAT CCCTCGCGCG AAGCATTTCT TTGAAGAAAG CCAAGAAGCC TCCCCTGCCA
    CCCTCTCGGA CGGACTCTCT GCGCAGGGTT CCCAAGAAGA ACGTCCAGTC GAACGGGCAG
    GTGCTGAACG AGAGCCTGAT CGCCTCTCTC CAGCACTCGC TGCAGCTGAA CCTCCCGGGC
    AAGGGCGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGATT TCGAAGAACC GTGGCTGCCT
    CGCTCCCGCA GCCAGAGCAC TGTGAGCGCC GGCAGCAGCC TAACCTCCGC CACGGCCCCC
    AACGTCTACT CCGTGTGCGG GCTCACCCCG GCACAGAGCG ACACGAGCAG CGTCAAGTCC
    GAGTACACAG ACCCGTGGGG TTACTACATT GACTACACGG GAGTACAGGA AGATCAGGGG
    AACGCAGGCA GGGCCGGTCC GGCCGGCAGC GCAGCATCAC CTGGAAGTGG GCCGGGCCGC
    CATCTCCTGG AGGGGTCCCG GGCTGCAGTA CCCCCAGTGC CCGGTGGCGC CGTCAAACCA
    AAGATCACAT CACCGGAGAA GTCGCACAGA GTCACTTCTC CATCCAGTGG GTATTCCAGT
    CAGTCGAACA CGCCCACCGC TCTCACCCCT GTGCCTGTGT TCTTAAAATC AATGTCACCA
    GCCAATGGGA AGGGAAAGCC CAAACCCAAG GTGCCAGAAA GGAAGTCGTC TCTTGTATCT
    TCAGTATCCG TCTCCTCCTC CTCCACGTCT CTTTCCTCGA ATACTTCCAC TGAAGGAAGC
    GGGACGGTGA AGAAGCTGGA TTCAGCGCTG GTGTCTCCGC TCGCGGCTGC TCCTCACACC
    TCTCCTCCAG CCCCTTGTCC CGCTGACAAG TCTCCTTTCC TCCCTCCCCC GCCACCTCTG
    GCAGATTTCT CCGAGGGCTC ACCTCTGCCT CGCTCTCCCC TCTTCCCCCC TCCGCCACCA
    GAAGTGCTCA CTCCCTTCTG TGCCCCCACC GACGCCTGCT TTCCTCCGCC CCCTACAGCA
    CTGAGCGCCC CTTTTCTGGA TTCATCTGAC TCCCTGCCAC CTCCGCCACC TGCCTTAACC
    CCTTCAGTGC CCCCGCCTGC CCCACCGCTT GACCCCAAAT TGATGAAAGA TGCCAGGCCT
    TCTTTCAAGA AACCTGGCCA GCCAGAGCCC TCCCGGGAGG CCTGCAGACA GCCTTCCATC
    AAGGAGGAGG GCAGCAGACT CCCCATGCCC CTGATCACCA CCGAAGCGTT GCAAATGGTG
    CAGTTGAGGC CGGTGAAGAA GAACTCAGGA ACCGAGGTAG CCCCCTTACA GGAACACGCA
    TCTCAGGAAA AACGAACTCC GATTGTTTCC CAGTATCATT TAAAGCCATC TGCTTTCCTG
    AAATCCCGAA ATAGCATACA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC CGCGACGAGC
    TCGCTCCCGA CTCCTGCCAA GAGCTCGACT CAGGGTCACC AGGACAGTGC AGCCGAGCAT
    GGCCTCCAGA GCCACAGCCC GGCCGGGGAG GCGGAGGCCG GAGCGGGTGC CACCCCGCCC
    CTCGGCCCGT CGCCCAGCAG GAAGCCGCCC CCCATTTCCA AGAAGCCCAA ACTGTTCCTC
    GTGGTGTCAC CTCCGCAGAG GGATTTCACG GCGGAGCCCG CAGAGAACGT GAGCGTGGCG
    TCTCCCAGCC CCACGAGCGG AGAGGCACGA GAGAGGGGTG CAGCAGGGGC TGGTTCCGAT
    GACACCGACT CTGGCAGCTC GGTGGTTCTT GTGGGAGGAG CTGCAGGATC CGAGTCCCCG
    GGCAGAGTGG AAGCCAATGT CCCCATGGTG CCAGCCCCTG CGGAGGAGCC GGGTGCCAGG
    AACGGTGTGG AGCGCTGCCT GCCTCCGCAG GACCCAGGGC CTGGGGTGCC GGAGGCTGAG
    ACAGCCACTT CTTCTTCAGA GACCTGTGAC TTCCCTAAGG ACGACGGGGG TGATGAGGTG
    ATGACCCCCA GTAGACCCAG GACCACAGAA GACCTTTTTG CAGCTATTCA CAGGTCTAAG
    AGGAAAGTCC TTGGCCGTAA AGATTCAGAG GATGATCACT CCCGAAACCA TTCTCCCTCC
    CCACCAGTGA CACCTACTGG TGCCGCCCCC AGCCTGGCCT CCCAGAAGCA AGTGGGGTCC
    ATTCAGAGAA GCGTCCGGAA GAGCAGCACC AGCAGTGACA ACTTCAAAGC TCTGCTGCTG
    AAAAAGGGGA GCCGCTCGGA TACCAGCGCC CGCATGTCAG CTGCAGAGAT GCTCAAAAAC
    ACAGACCCTA GATTCCAGAG GTCAAGGTCG GAGCCATCGC CAGACACGCC CGAGAGCCCG
    TCAAACTGCT CCCCGAGCAA GAACAGGAGG GCGCAGGAGG AGTGGGCCAA GACCGAAGGC
    TTGATGCCTC GGAGTCTGTC CTTCTCCGGC CCCAGGTACG GCCGCAGTCG AACGCCGCCT
    TCTGCCGCCA GCAGCAGGTA CAGCGTGCGG AACCGGATCC AGAGCAGCCC CATGACCGTC
    ATCTCGGAGG GCGAAGGGGA AGCCACGGAG CCCATAGACA GCACGGCTCT TCGGGCCCCG
    GGTGCTACAA GGGGGTGTTC GCTGGATGGA CTGGCAGGGG GTGGTGTGGA TGAGGGCAGC
    CTGCTCTGTG CCGGGGGACC CGCTGCCCCG CTGGAGGCCC AGGCTCCTGG TCCGGCAGTG
    GGGCTGCCCA GCGCAGAGGG CAGGGGCCCT TCAGAACAGC GAGGCGGTCT CGCCGGGGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852365.1
    CDS611..5239
    Translation

    Target ORF information:

    RefSeq Version XM_024996597.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCTGCC TGAAAGCTGT TTCCAACCTA GATGAGGAGA GTCGATGGAC GGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTCCCCA CCACAAGACC CCCCTGCGTG
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGA ATGTGATAAG
    TTACGGCGGG ATGGCTATCG GAGTTCTCAG TACTATTCCC AAGGACCCAC CTTTGCAGCC
    AACTCCAGTC CCCTCTGTGA TGATTACCAA GATGAGGATG AAGAAACAGA TCCAAAGTGT
    TCTGTCTCTT CATCAGAAGA GGAAAGACTT ACTTCCACCA GGAGACCTAA GACGCCAACG
    TCAAGTGACT GCTCTGACCT TCATACTCAA ACCAACTGGA CCAAGTCACT TCCCTTGCCA
    ACACCGGAAG AGAAGATGCG GCAGCAAGCC CAAACAGTCC AGGCTGACGT GGTTCCTATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAGCATAC AGAAGGAGCT AGCTTCGGGA GCCACTGGCC AAGATGATGT TGGTGGTCAC
    TCAGCATACA CCCCAGACCA CTACTCTACA CTCGGAAGGC TCGACAGCTA TCGATCTATG
    GGACAGCGCT CAGAAACCAG GGATTCCAGC TGTCAGACTG AGGAAGTGAA GGTCGTACCA
    CCTTCAATGA GAAGAATCAG AGCCCAGAAG GGGCAAGGCA TTGCTGCTCA GATGAGTCAC
    TTCTCAGGCT CCTCTGGGAA CATGTCAGTG CTGAGTGACT CTGCCGGGGT CGTGTTCCCT
    TCCCGCCTCA ACAGCGATGC TGGTTTTCAC AGTCTTCCCC GGTCTGGAGC AAGGGCAAAT
    GTTCAGGCCC TGGAGCCACG GCTGGGCGCC CTGGGCCCTG CGGAAGACCT GGGTGGCGCT
    TTCCCCTACC AGCAGGGTCA CCCACAAGTA GATGAAGACT TTGGGCATCT AGGAGCTGCC
    CCGAGGATGG GAACACTGTT GAGGCCCAAA TCTCAGGAGC TGAGGCACTT CGAGGGTGAA
    AATGTAACCA GCCCTGCGTG TGTGGTGTCT CCTCACACGA GCTACTCCAC CAGCATCATC
    CCAAATGCCA CGCTCTCCTC CTCTTCAGAG ATCATTGTGA TTCACACTGC TCAGAGCTCA
    GGGCAGCTGG ACAGTAAAAT TACCAGCTCA TCTTCATACT CAAAGGTCAG ATCCAGAGAC
    CACCTCCTCT CCAGGCAGAA AGATGGTCAT CATTCGTCCA GTGCAACCAC ACTGCTGTCC
    CTCTGTGACT CCGAAGTCTC TCTAAATACA CCAGCAAATC GGGAGAATGG GTCCCAGGCC
    ATGGCCTATA ACTGTAGAAA TAACCTTAGC ATCCCAGCCC ACCCCCAGGA TGTGGACAAC
    AAGAGTGAGT CCAGTTATTC AGGCAGCCGG GGCCAGGGTG GTAGCGTGGA GCCCTGGGAA
    TATGGTGGCT CAGGTAGTGG GCGGGCATCC CCACTGAAGC CACATTTGGC AGCTCCTGGT
    TACTCCACTC CTGTAAGCAA CACGAGCAGC TGCAGTTTGG ACCAGACCTC TAACAAGGAT
    GATACCAGGT CTGTGTATTC AGAGGACCAC GATGGCTACT CCCCTACTCT GCACCCGGAC
    TCTGGACACA GACCTGGGGA TCTGTGCAAT AGTAATGATG GCTTTGGGAA CCCCAGGCAC
    AGCGTGGTCA ATGTTTTTGA TGGAAGAGCT CAGAGACACC AAGGGGACCG GTCCAATTGC
    CAAGACAAAT CCCTCGCGCG AAGCATTTCT TTGAAGAAAG CCAAGAAGCC TCCCCTGCCA
    CCCTCTCGGA CGGACTCTCT GCGCAGGGTT CCCAAGAAGA ACGTCCAGTC GAACGGGCAG
    GTGCTGAACG AGAGCCTGAT CGCCTCTCTC CAGCACTCGC TGCAGCTGAA CCTCCCGGGC
    AAGGGCGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGATT TCGAAGAACC GTGGCTGCCT
    CGCTCCCGCA GCCAGAGCAC TGTGAGCGCC GGCAGCAGCC TAACCTCCGC CACGGCCCCC
    AACGTCTACT CCGTGTGCGG GCTCACCCCG GCACAGAGCG ACACGAGCAG CGTCAAGTCC
    GAGTACACAG ACCCGTGGGG TTACTACATT GACTACACGG GAGTACAGGA AGATCAGGGG
    AACGCAGGCA GGGCCGGTCC GGCCGGCAGC GCAGCATCAC CTGGAAGTGG GCCGGGCCGC
    CATCTCCTGG AGGGGTCCCG GGCTGCAGTA CCCCCAGTGC CCGGTGGCGC CGTCAAACCA
    AAGATCACAT CACCGGAGAA GTCGCACAGA GTCACTTCTC CATCCAGTGG GTATTCCAGT
    CAGTCGAACA CGCCCACCGC TCTCACCCCT GTGCCTGTGT TCTTAAAATC AATGTCACCA
    GCCAATGGGA AGGGAAAGCC CAAACCCAAG GTGCCAGAAA GGAAGTCGTC TCTTGTATCT
    TCAGTATCCG TCTCCTCCTC CTCCACGTCT CTTTCCTCGA ATACTTCCAC TGAAGGAAGC
    GGGACGGTGA AGAAGCTGGA TTCAGCGCTG GTGTCTCCGC TCGCGGCTGC TCCTCACACC
    TCTCCTCCAG CCCCTTGTCC CGCTGACAAG TCTCCTTTCC TCCCTCCCCC GCCACCTCTG
    GCAGATTTCT CCGAGGGCTC ACCTCTGCCT CGCTCTCCCC TCTTCCCCCC TCCGCCACCA
    GAAGTGCTCA CTCCCTTCTG TGCCCCCACC GACGCCTGCT TTCCTCCGCC CCCTACAGCA
    CTGAGCGCCC CTTTTCTGGA TTCATCTGAC TCCCTGCCAC CTCCGCCACC TGCCTTAACC
    CCTTCAGTGC CCCCGCCTGC CCCACCGCTT GACCCCAAAT TGATGAAAGA TGCCAGGCCT
    TCTTTCAAGA AACCTGGCCA GCCAGAGCCC TCCCGGGAGG CCTGCAGACA GCCTTCCATC
    AAGGAGGAGG GCAGCAGACT CCCCATGCCC CTGATCACCA CCGAAGCGTT GCAAATGGTG
    CAGTTGAGGC CGGTGAAGAA GAACTCAGGA ACCGAGGTAG CCCCCTTACA GGAACACGCA
    TCTCAGGAAA AACGAACTCC GATTGTTTCC CAGTATCATT TAAAGCCATC TGCTTTCCTG
    AAATCCCGAA ATAGCATACA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC CGCGACGAGC
    TCGCTCCCGA CTCCTGCCAA GAGCTCGACT CAGGGTCACC AGGACAGTGC AGCCGAGCAT
    GGCCTCCAGA GCCACAGCCC GGCCGGGGAG GCGGAGGCCG GAGCGGGTGC CACCCCGCCC
    CTCGGCCCGT CGCCCAGCAG GAAGCCGCCC CCCATTTCCA AGAAGCCCAA ACTGTTCCTC
    GTGGTGTCAC CTCCGCAGAG GGATTTCACG GCGGAGCCCG CAGAGAACGT GAGCGTGGCG
    TCTCCCAGCC CCACGAGCGG AGAGGCACGA GAGAGGGGTG CAGCAGGGGC TGGTTCCGAT
    GACACCGACT CTGGCAGCTC GGTGGTTCTT GTGGGAGGAG CTGCAGGATC CGAGTCCCCG
    GGCAGAGTGG AAGCCAATGT CCCCATGGTG CCAGCCCCTG CGGAGGAGCC GGGTGCCAGG
    AACGGTGTGG AGCGCTGCCT GCCTCCGCAG GACCCAGGGC CTGGGGTGCC GGAGGCTGAG
    ACAGCCACTT CTTCTTCAGA GACCTGTGAC TTCCCTAAGG ACGACGGGGG TGATGAGGTG
    ATGACCCCCA GTAGACCCAG GACCACAGAA GACCTTTTTG CAGCTATTCA CAGGTCTAAG
    AGGAAAGTCC TTGGCCGTAA AGATTCAGAG GATGATCACT CCCGAAACCA TTCTCCCTCC
    CCACCAGTGA CACCTACTGG TGCCGCCCCC AGCCTGGCCT CCCAGAAGCA AGTGGGGTCC
    ATTCAGAGAA GCGTCCGGAA GAGCAGCACC AGCAGTGACA ACTTCAAAGC TCTGCTGCTG
    AAAAAGGGGA GCCGCTCGGA TACCAGCGCC CGCATGTCAG CTGCAGAGAT GCTCAAAAAC
    ACAGACCCTA GATTCCAGAG GTCAAGGTCG GAGCCATCGC CAGACACGCC CGAGAGCCCG
    TCAAACTGCT CCCCGAGCAA GAACAGGAGG GCGCAGGAGG AGTGGGCCAA GACCGAAGGC
    TTGATGCCTC GGAGTCTGTC CTTCTCCGGC CCCAGGTACG GCCGCAGTCG AACGCCGCCT
    TCTGCCGCCA GCAGCAGGTA CAGCGTGCGG AACCGGATCC AGAGCAGCCC CATGACCGTC
    ATCTCGGAGG GCGAAGGGGA AGCCACGGAG CCCATAGACA GCACGGCTCT TCGGGCCCCG
    GGTGCTACAA GGGGGTGTTC GCTGGATGGA CTGGCAGGGG GTGGTGTGGA TGAGGGCAGC
    CTGCTCTGTG CCGGGGGACC CGCTGCCCCG CTGGAGGCCC AGGCTCCTGG TCCGGCAGTG
    GGGCTGCCCA GCGCAGAGGG CAGGGGCCCT TCAGAACAGC GAGGCGGTCT CGCCGGGGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91507
    Clone ID Related Accession (Same CDS sequence) XM_015472884.1
    Accession Version XM_015472884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCCTTCC ACCAGAGGAC CGTGGAGCCC GCGCGGCTGC GCCGGCCCGA GGCGGCCGGG 
    GCGGCGGGCG CGCCGCTCTT CCGCTCGCTG GAGCAGGTCA GCTCGCACGC CCTGGTCTGC
    CTGCTGGCGC AGCTCGCCGA CCTGTCGCGC TGCGCCGGGG ACATCTTCGG CGAGCTCGAG
    GGCCAGGCGG CTGCCCTGGG CCGCCGCACC GCCGCGCTGC ACCGCCGCCT CGACGCCCTG
    CACGCCGCCA CCGCGCGCCT GGACCACCGC CGAGTGAAAA TCCCTGTTTC CAACCTAGAT
    GAGGAGAGTC GATGGACGGT CCACTACACT GCTCCATGGC ACCAGCAGGA GAATGTGTTC
    CTCCCCACCA CAAGACCCCC CTGCGTGGAG GACCTGCACC GCCAAGCCAA GCTCAACCTC
    AAATCAGTAC TGAGGGAATG TGATAAGTTA CGGCGGGATG GCTATCGGAG TTCTCAGTAC
    TATTCCCAAG GACCCACCTT TGCAGCCAAC TCCAGTCCCC TCTGTGATGA TTACCAAGAT
    GAGGATGAAG AAACAGATCC AAAGTGTTCT GTCTCTTCAT CAGAAGAGGA AAGACTTACT
    TCCACCAGGA GACCTAAGAC GCCAACGTCA AGTGACTGCT CTGACCTTCA TACTCAAACC
    AACTGGACCA AGTCACTTCC CTTGCCAACA CCGGAAGAGA AGATGCGGCA GCAAGCCCAA
    ACAGTCCAGG CTGACGTGGT TCCTATTAAC ATAACTGGGG AGAATTTCGA TCGCCAGGCC
    AGCCTTCGGC GGTCTCTAAT TTACACAGAC ACTCTGGTAA GACGACCGAA GAAAGTCAAA
    AGGAGAAAGA CTATTACAGG AGTCCCTGAC AGCATACAGA AGGAGCTAGC TTCGGGAGCC
    ACTGGCCAAG ATGATGTTGG TGGTCACTCA GCATACACCC CAGACCACTA CTCTACACTC
    GGAAGGCTCG ACAGCTATCG ATCTATGGGA CAGCGCTCAG AAACCAGGGA TTCCAGCTGT
    CAGACTGAGG AAGTGAAGGT CGTACCACCT TCAATGAGAA GAATCAGAGC CCAGAAGGGG
    CAAGGCATTG CTGCTCAGAT GAGTCACTTC TCAGGCTCCT CTGGGAACAT GTCAGTGCTG
    AGTGACTCTG CCGGGGTCGT GTTCCCTTCC CGCCTCAACA GCGATGCTGG TTTTCACAGT
    CTTCCCCGGT CTGGAGCAAG GGCAAATGTT CAGGCCCTGG AGCCACGGCT GGGCGCCCTG
    GGCCCTGCGG AAGACCTGGG TGGCGCTTTC CCCTACCAGC AGGGTCACCC ACAAGTAGAT
    GAAGACTTTG GGCATCTAGG AGCTGCCCCG AGGATGGGAA CACTGTTGAG GCCCAAATCT
    CAGGAGCTGA GGCACTTCGA GGGTGAAAAT GTAACCAGCC CTGCGTGTGT GGTGTCTCCT
    CACACGAGCT ACTCCACCAG CATCATCCCA AATGCCACGC TCTCCTCCTC TTCAGAGATC
    ATTGTGATTC ACACTGCTCA GAGCTCAGGG CAGCTGGACA GTAAAATTAC CAGCTCATCT
    TCATACTCAA AGGTCAGATC CAGAGACCAC CTCCTCTCCA GGCAGAAAGA TGGTCATCAT
    TCGTCCAGTG CAACCACACT GCTGTCCCTC TGTGACTCCG AAGTCTCTCT AAATACACCA
    GCAAATCGGG AGAATGGGTC CCAGGCCATG GCCTATAACT GTAGAAATAA CCTTAGCATC
    CCAGCCCACC CCCAGGATGT GGACAACAAG AGTGAGTCCA GTTATTCAGG CAGCCGGGGC
    CAGGGTGGTA GCGTGGAGCC CTGGGAATAT GGTGGCTCAG GTAGTGGGCG GGCATCCCCA
    CTGAAGCCAC ATTTGGCAGC TCCTGGTTAC TCCACTCCTG TAAGCAACAC GAGCAGCTGC
    AGTTTGGACC AGACCTCTAA CAAGGATGAT ACCAGGTCTG TGTATTCAGA GGACCACGAT
    GGCTACTCCC CTACTCTGCA CCCGGACTCT GGACACAGAC CTGGGGATCT GTGCAATAGT
    AATGATGGCT TTGGGAACCC CAGGCACAGC GTGGTCAATG TTTTTGATGG AAGAGCTCAG
    AGACACCAAG GGGACCGGTC CAATTGCCAA GACAAATCCC TCGCGCGAAG CATTTCTTTG
    AAGAAAGCCA AGAAGCCTCC CCTGCCACCC TCTCGGACGG ACTCTCTGCG CAGGGTTCCC
    AAGAAGAACG TCCAGTCGAA CGGGCAGGTG CTGAACGAGA GCCTGATCGC CTCTCTCCAG
    CACTCGCTGC AGCTGAACCT CCCGGGCAAG GGCGGCAGCT CGCCCTCCCA GAGCCCCTGC
    AGTGATTTCG AAGAACCGTG GCTGCCTCGC TCCCGCAGCC AGAGCACTGT GAGCGCCGGC
    AGCAGCCTAA CCTCCGCCAC GGCCCCCAAC GTCTACTCCG TGTGCGGGCT CACCCCGGCA
    CAGAGCGACA CGAGCAGCGT CAAGTCCGAG TACACAGACC CGTGGGGTTA CTACATTGAC
    TACACGGGAG TACAGGAAGA TCAGGGGAAC GCAGGCAGGG CCGGTCCGGC CGGCAGCGCA
    GCATCACCTG GAAGTGGGCC GGGCCGCCAT CTCCTGGAGG GGTCCCGGGC TGCAGTACCC
    CCAGTGCCCG GTGGCGCCGT CAAACCAAAG ATCACATCAC CGGAGAAGTC GCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGTCAG TCGAACACGC CCACCGCTCT CACCCCTGTG
    CCTGTGTTCT TAAAATCAAT GTCACCAGCC AATGGGAAGG GAAAGCCCAA ACCCAAGGTG
    CCAGAAAGGA AGTCGTCTCT TGTATCTTCA GTATCCGTCT CCTCCTCCTC CACGTCTCTT
    TCCTCGAATA CTTCCACTGA AGGAAGCGGG ACGGTGAAGA AGCTGGATTC AGCGCTGGTG
    TCTCCGCTCG CGGCTGCTCC TCACACCTCT CCTCCAGCCC CTTGTCCCGC TGACAAGTCT
    CCTTTCCTCC CTCCCCCGCC ACCTCTGGCA GATTTCTCCG AGGGCTCACC TCTGCCTCGC
    TCTCCCCTCT TCCCCCCTCC GCCACCAGAA GTGCTCACTC CCTTCTGTGC CCCCACCGAC
    GCCTGCTTTC CTCCGCCCCC TACAGCACTG AGCGCCCCTT TTCTGGATTC ATCTGACTCC
    CTGCCACCTC CGCCACCTGC CTTAACCCCT TCAGTGCCCC CGCCTGCCCC ACCGCTTGAC
    CCCAAATTGA TGAAAGATGC CAGGCCTTCT TTCAAGAAAC CTGGCCAGCC AGAGCCCTCC
    CGGGAGGCCT GCAGACAGCC TTCCATCAAG GAGGAGGGCA GCAGACTCCC CATGCCCCTG
    ATCACCACCG AAGCGTTGCA AATGGTGCAG TTGAGGCCGG TGAAGAAGAA CTCAGGAACC
    GAGGTAGCCC CCTTACAGGA ACACGCATCT CAGGAAAAAC GAACTCCGAT TGTTTCCCAG
    TATCATTTAA AGCCATCTGC TTTCCTGAAA TCCCGAAATA GCATACATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCCGC GACGAGCTCG CTCCCGACTC CTGCCAAGAG CTCGACTCAG
    GGTCACCAGG ACAGTGCAGC CGAGCATGGC CTCCAGAGCC ACAGCCCGGC CGGGGAGGCG
    GAGGCCGGAG CGGGTGCCAC CCCGCCCCTC GGCCCGTCGC CCAGCAGGAA GCCGCCCCCC
    ATTTCCAAGA AGCCCAAACT GTTCCTCGTG GTGTCACCTC CGCAGAGGGA TTTCACGGCG
    GAGCCCGCAG AGAACGTGAG CGTGGCGTCT CCCAGCCCCA CGAGCGGAGA GGCACGAGAG
    AGGGGTGCAG CAGGGGCTGG TTCCGATGAC ACCGACTCTG GCAGCTCGGT GGTTCTTGTG
    GGAGGAGCTG CAGGATCCGA GTCCCCGGGC AGAGTGGAAG CCAATGTCCC CATGGTGCCA
    GCCCCTGCGG AGGAGCCGGG TGCCAGGAAC GGTGTGGAGC GCTGCCTGCC TCCGCAGGAC
    CCAGGGCCTG GGGTGCCGGA GGCTGAGACA GCCACTTCTT CTTCAGAGAC CTGTGACTTC
    CCTAAGGACG ACGGGGGTGA TGAGGTGATG ACCCCCAGTA GACCCAGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG GTCTAAGAGG AAAGTCCTTG GCCGTAAAGA TTCAGAGGAT
    GATCACTCCC GAAACCATTC TCCCTCCCCA CCAGTGACAC CTACTGGTGC CGCCCCCAGC
    CTGGCCTCCC AGAAGCAAGT GGGGTCCATT CAGAGAAGCG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTGAAA AAGGGGAGCC GCTCGGATAC CAGCGCCCGC
    ATGTCAGCTG CAGAGATGCT CAAAAACACA GACCCTAGAT TCCAGAGGTC AAGGTCGGAG
    CCATCGCCAG ACACGCCCGA GAGCCCGTCA AACTGCTCCC CGAGCAAGAA CAGGAGGGCG
    CAGGAGGAGT GGGCCAAGAC CGAAGGCTTG ATGCCTCGGA GTCTGTCCTT CTCCGGCCCC
    AGGTACGGCC GCAGTCGAAC GCCGCCTTCT GCCGCCAGCA GCAGGTACAG CGTGCGGAAC
    CGGATCCAGA GCAGCCCCAT GACCGTCATC TCGGAGGGCG AAGGGGAAGC CACGGAGCCC
    ATAGACAGCA CGGCTCTTCG GGCCCCGGGT GCTACAAGGG GGTGTTCGCT GGATGGACTG
    GCAGGGGGTG GTGTGGATGA GGGCAGCCTG CTCTGTGCCG GGGGACCCGC TGCCCCGCTG
    GAGGCCCAGG CTCCTGGTCC GGCAGTGGGG CTGCCCAGCG CAGAGGGCAG GGGCCCTTCA
    GAACAGCGAG GCGGTCTCGC CGGGGATAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328370.1
    CDS103..4998
    Translation

    Target ORF information:

    RefSeq Version XM_015472884.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCCTTCC ACCAGAGGAC CGTGGAGCCC GCGCGGCTGC GCCGGCCCGA GGCGGCCGGG 
    GCGGCGGGCG CGCCGCTCTT CCGCTCGCTG GAGCAGGTCA GCTCGCACGC CCTGGTCTGC
    CTGCTGGCGC AGCTCGCCGA CCTGTCGCGC TGCGCCGGGG ACATCTTCGG CGAGCTCGAG
    GGCCAGGCGG CTGCCCTGGG CCGCCGCACC GCCGCGCTGC ACCGCCGCCT CGACGCCCTG
    CACGCCGCCA CCGCGCGCCT GGACCACCGC CGAGTGAAAA TCCCTGTTTC CAACCTAGAT
    GAGGAGAGTC GATGGACGGT CCACTACACT GCTCCATGGC ACCAGCAGGA GAATGTGTTC
    CTCCCCACCA CAAGACCCCC CTGCGTGGAG GACCTGCACC GCCAAGCCAA GCTCAACCTC
    AAATCAGTAC TGAGGGAATG TGATAAGTTA CGGCGGGATG GCTATCGGAG TTCTCAGTAC
    TATTCCCAAG GACCCACCTT TGCAGCCAAC TCCAGTCCCC TCTGTGATGA TTACCAAGAT
    GAGGATGAAG AAACAGATCC AAAGTGTTCT GTCTCTTCAT CAGAAGAGGA AAGACTTACT
    TCCACCAGGA GACCTAAGAC GCCAACGTCA AGTGACTGCT CTGACCTTCA TACTCAAACC
    AACTGGACCA AGTCACTTCC CTTGCCAACA CCGGAAGAGA AGATGCGGCA GCAAGCCCAA
    ACAGTCCAGG CTGACGTGGT TCCTATTAAC ATAACTGGGG AGAATTTCGA TCGCCAGGCC
    AGCCTTCGGC GGTCTCTAAT TTACACAGAC ACTCTGGTAA GACGACCGAA GAAAGTCAAA
    AGGAGAAAGA CTATTACAGG AGTCCCTGAC AGCATACAGA AGGAGCTAGC TTCGGGAGCC
    ACTGGCCAAG ATGATGTTGG TGGTCACTCA GCATACACCC CAGACCACTA CTCTACACTC
    GGAAGGCTCG ACAGCTATCG ATCTATGGGA CAGCGCTCAG AAACCAGGGA TTCCAGCTGT
    CAGACTGAGG AAGTGAAGGT CGTACCACCT TCAATGAGAA GAATCAGAGC CCAGAAGGGG
    CAAGGCATTG CTGCTCAGAT GAGTCACTTC TCAGGCTCCT CTGGGAACAT GTCAGTGCTG
    AGTGACTCTG CCGGGGTCGT GTTCCCTTCC CGCCTCAACA GCGATGCTGG TTTTCACAGT
    CTTCCCCGGT CTGGAGCAAG GGCAAATGTT CAGGCCCTGG AGCCACGGCT GGGCGCCCTG
    GGCCCTGCGG AAGACCTGGG TGGCGCTTTC CCCTACCAGC AGGGTCACCC ACAAGTAGAT
    GAAGACTTTG GGCATCTAGG AGCTGCCCCG AGGATGGGAA CACTGTTGAG GCCCAAATCT
    CAGGAGCTGA GGCACTTCGA GGGTGAAAAT GTAACCAGCC CTGCGTGTGT GGTGTCTCCT
    CACACGAGCT ACTCCACCAG CATCATCCCA AATGCCACGC TCTCCTCCTC TTCAGAGATC
    ATTGTGATTC ACACTGCTCA GAGCTCAGGG CAGCTGGACA GTAAAATTAC CAGCTCATCT
    TCATACTCAA AGGTCAGATC CAGAGACCAC CTCCTCTCCA GGCAGAAAGA TGGTCATCAT
    TCGTCCAGTG CAACCACACT GCTGTCCCTC TGTGACTCCG AAGTCTCTCT AAATACACCA
    GCAAATCGGG AGAATGGGTC CCAGGCCATG GCCTATAACT GTAGAAATAA CCTTAGCATC
    CCAGCCCACC CCCAGGATGT GGACAACAAG AGTGAGTCCA GTTATTCAGG CAGCCGGGGC
    CAGGGTGGTA GCGTGGAGCC CTGGGAATAT GGTGGCTCAG GTAGTGGGCG GGCATCCCCA
    CTGAAGCCAC ATTTGGCAGC TCCTGGTTAC TCCACTCCTG TAAGCAACAC GAGCAGCTGC
    AGTTTGGACC AGACCTCTAA CAAGGATGAT ACCAGGTCTG TGTATTCAGA GGACCACGAT
    GGCTACTCCC CTACTCTGCA CCCGGACTCT GGACACAGAC CTGGGGATCT GTGCAATAGT
    AATGATGGCT TTGGGAACCC CAGGCACAGC GTGGTCAATG TTTTTGATGG AAGAGCTCAG
    AGACACCAAG GGGACCGGTC CAATTGCCAA GACAAATCCC TCGCGCGAAG CATTTCTTTG
    AAGAAAGCCA AGAAGCCTCC CCTGCCACCC TCTCGGACGG ACTCTCTGCG CAGGGTTCCC
    AAGAAGAACG TCCAGTCGAA CGGGCAGGTG CTGAACGAGA GCCTGATCGC CTCTCTCCAG
    CACTCGCTGC AGCTGAACCT CCCGGGCAAG GGCGGCAGCT CGCCCTCCCA GAGCCCCTGC
    AGTGATTTCG AAGAACCGTG GCTGCCTCGC TCCCGCAGCC AGAGCACTGT GAGCGCCGGC
    AGCAGCCTAA CCTCCGCCAC GGCCCCCAAC GTCTACTCCG TGTGCGGGCT CACCCCGGCA
    CAGAGCGACA CGAGCAGCGT CAAGTCCGAG TACACAGACC CGTGGGGTTA CTACATTGAC
    TACACGGGAG TACAGGAAGA TCAGGGGAAC GCAGGCAGGG CCGGTCCGGC CGGCAGCGCA
    GCATCACCTG GAAGTGGGCC GGGCCGCCAT CTCCTGGAGG GGTCCCGGGC TGCAGTACCC
    CCAGTGCCCG GTGGCGCCGT CAAACCAAAG ATCACATCAC CGGAGAAGTC GCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGTCAG TCGAACACGC CCACCGCTCT CACCCCTGTG
    CCTGTGTTCT TAAAATCAAT GTCACCAGCC AATGGGAAGG GAAAGCCCAA ACCCAAGGTG
    CCAGAAAGGA AGTCGTCTCT TGTATCTTCA GTATCCGTCT CCTCCTCCTC CACGTCTCTT
    TCCTCGAATA CTTCCACTGA AGGAAGCGGG ACGGTGAAGA AGCTGGATTC AGCGCTGGTG
    TCTCCGCTCG CGGCTGCTCC TCACACCTCT CCTCCAGCCC CTTGTCCCGC TGACAAGTCT
    CCTTTCCTCC CTCCCCCGCC ACCTCTGGCA GATTTCTCCG AGGGCTCACC TCTGCCTCGC
    TCTCCCCTCT TCCCCCCTCC GCCACCAGAA GTGCTCACTC CCTTCTGTGC CCCCACCGAC
    GCCTGCTTTC CTCCGCCCCC TACAGCACTG AGCGCCCCTT TTCTGGATTC ATCTGACTCC
    CTGCCACCTC CGCCACCTGC CTTAACCCCT TCAGTGCCCC CGCCTGCCCC ACCGCTTGAC
    CCCAAATTGA TGAAAGATGC CAGGCCTTCT TTCAAGAAAC CTGGCCAGCC AGAGCCCTCC
    CGGGAGGCCT GCAGACAGCC TTCCATCAAG GAGGAGGGCA GCAGACTCCC CATGCCCCTG
    ATCACCACCG AAGCGTTGCA AATGGTGCAG TTGAGGCCGG TGAAGAAGAA CTCAGGAACC
    GAGGTAGCCC CCTTACAGGA ACACGCATCT CAGGAAAAAC GAACTCCGAT TGTTTCCCAG
    TATCATTTAA AGCCATCTGC TTTCCTGAAA TCCCGAAATA GCATACATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCCGC GACGAGCTCG CTCCCGACTC CTGCCAAGAG CTCGACTCAG
    GGTCACCAGG ACAGTGCAGC CGAGCATGGC CTCCAGAGCC ACAGCCCGGC CGGGGAGGCG
    GAGGCCGGAG CGGGTGCCAC CCCGCCCCTC GGCCCGTCGC CCAGCAGGAA GCCGCCCCCC
    ATTTCCAAGA AGCCCAAACT GTTCCTCGTG GTGTCACCTC CGCAGAGGGA TTTCACGGCG
    GAGCCCGCAG AGAACGTGAG CGTGGCGTCT CCCAGCCCCA CGAGCGGAGA GGCACGAGAG
    AGGGGTGCAG CAGGGGCTGG TTCCGATGAC ACCGACTCTG GCAGCTCGGT GGTTCTTGTG
    GGAGGAGCTG CAGGATCCGA GTCCCCGGGC AGAGTGGAAG CCAATGTCCC CATGGTGCCA
    GCCCCTGCGG AGGAGCCGGG TGCCAGGAAC GGTGTGGAGC GCTGCCTGCC TCCGCAGGAC
    CCAGGGCCTG GGGTGCCGGA GGCTGAGACA GCCACTTCTT CTTCAGAGAC CTGTGACTTC
    CCTAAGGACG ACGGGGGTGA TGAGGTGATG ACCCCCAGTA GACCCAGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG GTCTAAGAGG AAAGTCCTTG GCCGTAAAGA TTCAGAGGAT
    GATCACTCCC GAAACCATTC TCCCTCCCCA CCAGTGACAC CTACTGGTGC CGCCCCCAGC
    CTGGCCTCCC AGAAGCAAGT GGGGTCCATT CAGAGAAGCG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTGAAA AAGGGGAGCC GCTCGGATAC CAGCGCCCGC
    ATGTCAGCTG CAGAGATGCT CAAAAACACA GACCCTAGAT TCCAGAGGTC AAGGTCGGAG
    CCATCGCCAG ACACGCCCGA GAGCCCGTCA AACTGCTCCC CGAGCAAGAA CAGGAGGGCG
    CAGGAGGAGT GGGCCAAGAC CGAAGGCTTG ATGCCTCGGA GTCTGTCCTT CTCCGGCCCC
    AGGTACGGCC GCAGTCGAAC GCCGCCTTCT GCCGCCAGCA GCAGGTACAG CGTGCGGAAC
    CGGATCCAGA GCAGCCCCAT GACCGTCATC TCGGAGGGCG AAGGGGAAGC CACGGAGCCC
    ATAGACAGCA CGGCTCTTCG GGCCCCGGGT GCTACAAGGG GGTGTTCGCT GGATGGACTG
    GCAGGGGGTG GTGTGGATGA GGGCAGCCTG CTCTGTGCCG GGGGACCCGC TGCCCCGCTG
    GAGGCCCAGG CTCCTGGTCC GGCAGTGGGG CTGCCCAGCG CAGAGGGCAG GGGCCCTTCA
    GAACAGCGAG GCGGTCTCGC CGGGGATAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91508
    Clone ID Related Accession (Same CDS sequence) XM_015472885.1
    Accession Version XM_015472885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCCTTCC ACCAGAGGAC CGTGGAGCCC GCGCGGCTGC GCCGGCCCGA GGCGGCCGGG 
    GCGGCGGGCG CGCCGCTCTT CCGCTCGCTG GAGCAGGTCA GCTCGCACGC CCTGGTCTGC
    CTGCTGGCGC AGCTCGCCGA CCTGTCGCGC TGCGCCGGGG ACATCTTCGG CGAGCTCGAG
    GGCCAGGCGG CTGCCCTGGG CCGCCGCACC GCCGCGCTGC ACCGCCGCCT CGACGCCCTG
    CACGCCGCCA CCGCGCGCCT GGACCACCGC CGAGTGAAAA TCCCTGTTTC CAACCTAGAT
    GAGGAGAGTC GATGGACGGT CCACTACACT GCTCCATGGC ACCAGCAGGA GAATGTGTTC
    CTCCCCACCA CAAGACCCCC CTGCGTGGAG GACCTGCACC GCCAAGCCAA GCTCAACCTC
    AAATCAGTAC TGAGGGAATG TGATAAGTTA CGGCGGGATG GCTATCGGAG TTCTCAGTAC
    TATTCCCAAG GACCCACCTT TGCAGCCAAC TCCAGTCCCC TCTGTGATGA TTACCAAGAT
    GAGGATGAAG AAACAGATCC AAAGTGTTCT GTCTCTTCAT CAGAAGAGGA AAGACTTACT
    TCCACCAGGA GACCTAAGAC GCCAACGTCA AGTGACTGCT CTGACCTTCA TACTCAAACC
    AACTGGACCA AGTCACTTCC CTTGCCAACA CCGGAAGAGA AGATGCGGCA GCAAGCCCAA
    ACAGTCCAGG CTGACGTGGT TCCTATTAAC ATAACTGCTT CGGGAGCCAC TGGCCAAGAT
    GATGTTGGTG GTCACTCAGC ATACACCCCA GACCACTACT CTACACTCGG AAGGCTCGAC
    AGCTATCGAT CTATGGGACA GCGCTCAGAA ACCAGGGATT CCAGCTGTCA GACTGAGGAA
    GTGAAGGTCG TACCACCTTC AATGAGAAGA ATCAGAGCCC AGAAGGGGCA AGGCATTGCT
    GCTCAGATGA GTCACTTCTC AGGCTCCTCT GGGAACATGT CAGTGCTGAG TGACTCTGCC
    GGGGTCGTGT TCCCTTCCCG CCTCAACAGC GATGCTGGTT TTCACAGTCT TCCCCGGTCT
    GGAGCAAGGG CAAATGTTCA GGCCCTGGAG CCACGGCTGG GCGCCCTGGG CCCTGCGGAA
    GACCTGGGTG GCGCTTTCCC CTACCAGCAG GGTCACCCAC AAGTAGATGA AGACTTTGGG
    CATCTAGGAG CTGCCCCGAG GATGGGAACA CTGTTGAGGC CCAAATCTCA GGAGCTGAGG
    CACTTCGAGG GTGAAAATGT AACCAGCCCT GCGTGTGTGG TGTCTCCTCA CACGAGCTAC
    TCCACCAGCA TCATCCCAAA TGCCACGCTC TCCTCCTCTT CAGAGATCAT TGTGATTCAC
    ACTGCTCAGA GCTCAGGGCA GCTGGACAGT AAAATTACCA GCTCATCTTC ATACTCAAAG
    GTCAGATCCA GAGACCACCT CCTCTCCAGG CAGAAAGATG GTCATCATTC GTCCAGTGCA
    ACCACACTGC TGTCCCTCTG TGACTCCGAA GTCTCTCTAA ATACACCAGC AAATCGGGAG
    AATGGGTCCC AGGCCATGGC CTATAACTGT AGAAATAACC TTAGCATCCC AGCCCACCCC
    CAGGATGTGG ACAACAAGAG TGAGTCCAGT TATTCAGGCA GCCGGGGCCA GGGTGGTAGC
    GTGGAGCCCT GGGAATATGG TGGCTCAGGT AGTGGGCGGG CATCCCCACT GAAGCCACAT
    TTGGCAGCTC CTGGTTACTC CACTCCTGTA AGCAACACGA GCAGCTGCAG TTTGGACCAG
    ACCTCTAACA AGGATGATAC CAGGTCTGTG TATTCAGAGG ACCACGATGG CTACTCCCCT
    ACTCTGCACC CGGACTCTGG ACACAGACCT GGGGATCTGT GCAATAGTAA TGATGGCTTT
    GGGAACCCCA GGCACAGCGT GGTCAATGTT TTTGATGGAA GAGCTCAGAG ACACCAAGGG
    GACCGGTCCA ATTGCCAAGA CAAATCCCTC GCGCGAAGCA TTTCTTTGAA GAAAGCCAAG
    AAGCCTCCCC TGCCACCCTC TCGGACGGAC TCTCTGCGCA GGGTTCCCAA GAAGAACGTC
    CAGTCGAACG GGCAGGTGCT GAACGAGAGC CTGATCGCCT CTCTCCAGCA CTCGCTGCAG
    CTGAACCTCC CGGGCAAGGG CGGCAGCTCG CCCTCCCAGA GCCCCTGCAG TGATTTCGAA
    GAACCGTGGC TGCCTCGCTC CCGCAGCCAG AGCACTGTGA GCGCCGGCAG CAGCCTAACC
    TCCGCCACGG CCCCCAACGT CTACTCCGTG TGCGGGCTCA CCCCGGCACA GAGCGACACG
    AGCAGCGTCA AGTCCGAGTA CACAGACCCG TGGGGTTACT ACATTGACTA CACGGGAGTA
    CAGGAAGATC AGGGGAACGC AGGCAGGGCC GGTCCGGCCG GCAGCGCAGC ATCACCTGGA
    AGTGGGCCGG GCCGCCATCT CCTGGAGGGG TCCCGGGCTG CAGTACCCCC AGTGCCCGGT
    GGCGCCGTCA AACCAAAGAT CACATCACCG GAGAAGTCGC ACAGAGTCAC TTCTCCATCC
    AGTGGGTATT CCAGTCAGTC GAACACGCCC ACCGCTCTCA CCCCTGTGCC TGTGTTCTTA
    AAATCAATGT CACCAGCCAA TGGGAAGGGA AAGCCCAAAC CCAAGGTGCC AGAAAGGAAG
    TCGTCTCTTG TATCTTCAGT ATCCGTCTCC TCCTCCTCCA CGTCTCTTTC CTCGAATACT
    TCCACTGAAG GAAGCGGGAC GGTGAAGAAG CTGGATTCAG CGCTGGTGTC TCCGCTCGCG
    GCTGCTCCTC ACACCTCTCC TCCAGCCCCT TGTCCCGCTG ACAAGTCTCC TTTCCTCCCT
    CCCCCGCCAC CTCTGGCAGA TTTCTCCGAG GGCTCACCTC TGCCTCGCTC TCCCCTCTTC
    CCCCCTCCGC CACCAGAAGT GCTCACTCCC TTCTGTGCCC CCACCGACGC CTGCTTTCCT
    CCGCCCCCTA CAGCACTGAG CGCCCCTTTT CTGGATTCAT CTGACTCCCT GCCACCTCCG
    CCACCTGCCT TAACCCCTTC AGTGCCCCCG CCTGCCCCAC CGCTTGACCC CAAATTGATG
    AAAGATGCCA GGCCTTCTTT CAAGAAACCT GGCCAGCCAG AGCCCTCCCG GGAGGCCTGC
    AGACAGCCTT CCATCAAGGA GGAGGGCAGC AGACTCCCCA TGCCCCTGAT CACCACCGAA
    GCGTTGCAAA TGGTGCAGTT GAGGCCGGTG AAGAAGAACT CAGGAACCGA GGTAGCCCCC
    TTACAGGAAC ACGCATCTCA GGAAAAACGA ACTCCGATTG TTTCCCAGTA TCATTTAAAG
    CCATCTGCTT TCCTGAAATC CCGAAATAGC ATACATGAAA TGGAGAGTGA AAGCCAGCCT
    GCCTCCGCGA CGAGCTCGCT CCCGACTCCT GCCAAGAGCT CGACTCAGGG TCACCAGGAC
    AGTGCAGCCG AGCATGGCCT CCAGAGCCAC AGCCCGGCCG GGGAGGCGGA GGCCGGAGCG
    GGTGCCACCC CGCCCCTCGG CCCGTCGCCC AGCAGGAAGC CGCCCCCCAT TTCCAAGAAG
    CCCAAACTGT TCCTCGTGGT GTCACCTCCG CAGAGGGATT TCACGGCGGA GCCCGCAGAG
    AACGTGAGCG TGGCGTCTCC CAGCCCCACG AGCGGAGAGG CACGAGAGAG GGGTGCAGCA
    GGGGCTGGTT CCGATGACAC CGACTCTGGC AGCTCGGTGG TTCTTGTGGG AGGAGCTGCA
    GGATCCGAGT CCCCGGGCAG AGTGGAAGCC AATGTCCCCA TGGTGCCAGC CCCTGCGGAG
    GAGCCGGGTG CCAGGAACGG TGTGGAGCGC TGCCTGCCTC CGCAGGACCC AGGGCCTGGG
    GTGCCGGAGG CTGAGACAGC CACTTCTTCT TCAGAGACCT GTGACTTCCC TAAGGACGAC
    GGGGGTGATG AGGTGATGAC CCCCAGTAGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT
    ATTCACAGGT CTAAGAGGAA AGTCCTTGGC CGTAAAGATT CAGAGGATGA TCACTCCCGA
    AACCATTCTC CCTCCCCACC AGTGACACCT ACTGGTGCCG CCCCCAGCCT GGCCTCCCAG
    AAGCAAGTGG GGTCCATTCA GAGAAGCGTC CGGAAGAGCA GCACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTGAAAAA GGGGAGCCGC TCGGATACCA GCGCCCGCAT GTCAGCTGCA
    GAGATGCTCA AAAACACAGA CCCTAGATTC CAGAGGTCAA GGTCGGAGCC ATCGCCAGAC
    ACGCCCGAGA GCCCGTCAAA CTGCTCCCCG AGCAAGAACA GGAGGGCGCA GGAGGAGTGG
    GCCAAGACCG AAGGCTTGAT GCCTCGGAGT CTGTCCTTCT CCGGCCCCAG GTACGGCCGC
    AGTCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCG TGCGGAACCG GATCCAGAGC
    AGCCCCATGA CCGTCATCTC GGAGGGCGAA GGGGAAGCCA CGGAGCCCAT AGACAGCACG
    GCTCTTCGGG CCCCGGGTGC TACAAGGGGG TGTTCGCTGG ATGGACTGGC AGGGGGTGGT
    GTGGATGAGG GCAGCCTGCT CTGTGCCGGG GGACCCGCTG CCCCGCTGGA GGCCCAGGCT
    CCTGGTCCGG CAGTGGGGCT GCCCAGCGCA GAGGGCAGGG GCCCTTCAGA ACAGCGAGGC
    GGTCTCGCCG GGGATAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328371.1
    CDS103..4866
    Translation

    Target ORF information:

    RefSeq Version XM_015472885.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 1 (NHSL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCCTTCC ACCAGAGGAC CGTGGAGCCC GCGCGGCTGC GCCGGCCCGA GGCGGCCGGG 
    GCGGCGGGCG CGCCGCTCTT CCGCTCGCTG GAGCAGGTCA GCTCGCACGC CCTGGTCTGC
    CTGCTGGCGC AGCTCGCCGA CCTGTCGCGC TGCGCCGGGG ACATCTTCGG CGAGCTCGAG
    GGCCAGGCGG CTGCCCTGGG CCGCCGCACC GCCGCGCTGC ACCGCCGCCT CGACGCCCTG
    CACGCCGCCA CCGCGCGCCT GGACCACCGC CGAGTGAAAA TCCCTGTTTC CAACCTAGAT
    GAGGAGAGTC GATGGACGGT CCACTACACT GCTCCATGGC ACCAGCAGGA GAATGTGTTC
    CTCCCCACCA CAAGACCCCC CTGCGTGGAG GACCTGCACC GCCAAGCCAA GCTCAACCTC
    AAATCAGTAC TGAGGGAATG TGATAAGTTA CGGCGGGATG GCTATCGGAG TTCTCAGTAC
    TATTCCCAAG GACCCACCTT TGCAGCCAAC TCCAGTCCCC TCTGTGATGA TTACCAAGAT
    GAGGATGAAG AAACAGATCC AAAGTGTTCT GTCTCTTCAT CAGAAGAGGA AAGACTTACT
    TCCACCAGGA GACCTAAGAC GCCAACGTCA AGTGACTGCT CTGACCTTCA TACTCAAACC
    AACTGGACCA AGTCACTTCC CTTGCCAACA CCGGAAGAGA AGATGCGGCA GCAAGCCCAA
    ACAGTCCAGG CTGACGTGGT TCCTATTAAC ATAACTGCTT CGGGAGCCAC TGGCCAAGAT
    GATGTTGGTG GTCACTCAGC ATACACCCCA GACCACTACT CTACACTCGG AAGGCTCGAC
    AGCTATCGAT CTATGGGACA GCGCTCAGAA ACCAGGGATT CCAGCTGTCA GACTGAGGAA
    GTGAAGGTCG TACCACCTTC AATGAGAAGA ATCAGAGCCC AGAAGGGGCA AGGCATTGCT
    GCTCAGATGA GTCACTTCTC AGGCTCCTCT GGGAACATGT CAGTGCTGAG TGACTCTGCC
    GGGGTCGTGT TCCCTTCCCG CCTCAACAGC GATGCTGGTT TTCACAGTCT TCCCCGGTCT
    GGAGCAAGGG CAAATGTTCA GGCCCTGGAG CCACGGCTGG GCGCCCTGGG CCCTGCGGAA
    GACCTGGGTG GCGCTTTCCC CTACCAGCAG GGTCACCCAC AAGTAGATGA AGACTTTGGG
    CATCTAGGAG CTGCCCCGAG GATGGGAACA CTGTTGAGGC CCAAATCTCA GGAGCTGAGG
    CACTTCGAGG GTGAAAATGT AACCAGCCCT GCGTGTGTGG TGTCTCCTCA CACGAGCTAC
    TCCACCAGCA TCATCCCAAA TGCCACGCTC TCCTCCTCTT CAGAGATCAT TGTGATTCAC
    ACTGCTCAGA GCTCAGGGCA GCTGGACAGT AAAATTACCA GCTCATCTTC ATACTCAAAG
    GTCAGATCCA GAGACCACCT CCTCTCCAGG CAGAAAGATG GTCATCATTC GTCCAGTGCA
    ACCACACTGC TGTCCCTCTG TGACTCCGAA GTCTCTCTAA ATACACCAGC AAATCGGGAG
    AATGGGTCCC AGGCCATGGC CTATAACTGT AGAAATAACC TTAGCATCCC AGCCCACCCC
    CAGGATGTGG ACAACAAGAG TGAGTCCAGT TATTCAGGCA GCCGGGGCCA GGGTGGTAGC
    GTGGAGCCCT GGGAATATGG TGGCTCAGGT AGTGGGCGGG CATCCCCACT GAAGCCACAT
    TTGGCAGCTC CTGGTTACTC CACTCCTGTA AGCAACACGA GCAGCTGCAG TTTGGACCAG
    ACCTCTAACA AGGATGATAC CAGGTCTGTG TATTCAGAGG ACCACGATGG CTACTCCCCT
    ACTCTGCACC CGGACTCTGG ACACAGACCT GGGGATCTGT GCAATAGTAA TGATGGCTTT
    GGGAACCCCA GGCACAGCGT GGTCAATGTT TTTGATGGAA GAGCTCAGAG ACACCAAGGG
    GACCGGTCCA ATTGCCAAGA CAAATCCCTC GCGCGAAGCA TTTCTTTGAA GAAAGCCAAG
    AAGCCTCCCC TGCCACCCTC TCGGACGGAC TCTCTGCGCA GGGTTCCCAA GAAGAACGTC
    CAGTCGAACG GGCAGGTGCT GAACGAGAGC CTGATCGCCT CTCTCCAGCA CTCGCTGCAG
    CTGAACCTCC CGGGCAAGGG CGGCAGCTCG CCCTCCCAGA GCCCCTGCAG TGATTTCGAA
    GAACCGTGGC TGCCTCGCTC CCGCAGCCAG AGCACTGTGA GCGCCGGCAG CAGCCTAACC
    TCCGCCACGG CCCCCAACGT CTACTCCGTG TGCGGGCTCA CCCCGGCACA GAGCGACACG
    AGCAGCGTCA AGTCCGAGTA CACAGACCCG TGGGGTTACT ACATTGACTA CACGGGAGTA
    CAGGAAGATC AGGGGAACGC AGGCAGGGCC GGTCCGGCCG GCAGCGCAGC ATCACCTGGA
    AGTGGGCCGG GCCGCCATCT CCTGGAGGGG TCCCGGGCTG CAGTACCCCC AGTGCCCGGT
    GGCGCCGTCA AACCAAAGAT CACATCACCG GAGAAGTCGC ACAGAGTCAC TTCTCCATCC
    AGTGGGTATT CCAGTCAGTC GAACACGCCC ACCGCTCTCA CCCCTGTGCC TGTGTTCTTA
    AAATCAATGT CACCAGCCAA TGGGAAGGGA AAGCCCAAAC CCAAGGTGCC AGAAAGGAAG
    TCGTCTCTTG TATCTTCAGT ATCCGTCTCC TCCTCCTCCA CGTCTCTTTC CTCGAATACT
    TCCACTGAAG GAAGCGGGAC GGTGAAGAAG CTGGATTCAG CGCTGGTGTC TCCGCTCGCG
    GCTGCTCCTC ACACCTCTCC TCCAGCCCCT TGTCCCGCTG ACAAGTCTCC TTTCCTCCCT
    CCCCCGCCAC CTCTGGCAGA TTTCTCCGAG GGCTCACCTC TGCCTCGCTC TCCCCTCTTC
    CCCCCTCCGC CACCAGAAGT GCTCACTCCC TTCTGTGCCC CCACCGACGC CTGCTTTCCT
    CCGCCCCCTA CAGCACTGAG CGCCCCTTTT CTGGATTCAT CTGACTCCCT GCCACCTCCG
    CCACCTGCCT TAACCCCTTC AGTGCCCCCG CCTGCCCCAC CGCTTGACCC CAAATTGATG
    AAAGATGCCA GGCCTTCTTT CAAGAAACCT GGCCAGCCAG AGCCCTCCCG GGAGGCCTGC
    AGACAGCCTT CCATCAAGGA GGAGGGCAGC AGACTCCCCA TGCCCCTGAT CACCACCGAA
    GCGTTGCAAA TGGTGCAGTT GAGGCCGGTG AAGAAGAACT CAGGAACCGA GGTAGCCCCC
    TTACAGGAAC ACGCATCTCA GGAAAAACGA ACTCCGATTG TTTCCCAGTA TCATTTAAAG
    CCATCTGCTT TCCTGAAATC CCGAAATAGC ATACATGAAA TGGAGAGTGA AAGCCAGCCT
    GCCTCCGCGA CGAGCTCGCT CCCGACTCCT GCCAAGAGCT CGACTCAGGG TCACCAGGAC
    AGTGCAGCCG AGCATGGCCT CCAGAGCCAC AGCCCGGCCG GGGAGGCGGA GGCCGGAGCG
    GGTGCCACCC CGCCCCTCGG CCCGTCGCCC AGCAGGAAGC CGCCCCCCAT TTCCAAGAAG
    CCCAAACTGT TCCTCGTGGT GTCACCTCCG CAGAGGGATT TCACGGCGGA GCCCGCAGAG
    AACGTGAGCG TGGCGTCTCC CAGCCCCACG AGCGGAGAGG CACGAGAGAG GGGTGCAGCA
    GGGGCTGGTT CCGATGACAC CGACTCTGGC AGCTCGGTGG TTCTTGTGGG AGGAGCTGCA
    GGATCCGAGT CCCCGGGCAG AGTGGAAGCC AATGTCCCCA TGGTGCCAGC CCCTGCGGAG
    GAGCCGGGTG CCAGGAACGG TGTGGAGCGC TGCCTGCCTC CGCAGGACCC AGGGCCTGGG
    GTGCCGGAGG CTGAGACAGC CACTTCTTCT TCAGAGACCT GTGACTTCCC TAAGGACGAC
    GGGGGTGATG AGGTGATGAC CCCCAGTAGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT
    ATTCACAGGT CTAAGAGGAA AGTCCTTGGC CGTAAAGATT CAGAGGATGA TCACTCCCGA
    AACCATTCTC CCTCCCCACC AGTGACACCT ACTGGTGCCG CCCCCAGCCT GGCCTCCCAG
    AAGCAAGTGG GGTCCATTCA GAGAAGCGTC CGGAAGAGCA GCACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTGAAAAA GGGGAGCCGC TCGGATACCA GCGCCCGCAT GTCAGCTGCA
    GAGATGCTCA AAAACACAGA CCCTAGATTC CAGAGGTCAA GGTCGGAGCC ATCGCCAGAC
    ACGCCCGAGA GCCCGTCAAA CTGCTCCCCG AGCAAGAACA GGAGGGCGCA GGAGGAGTGG
    GCCAAGACCG AAGGCTTGAT GCCTCGGAGT CTGTCCTTCT CCGGCCCCAG GTACGGCCGC
    AGTCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCG TGCGGAACCG GATCCAGAGC
    AGCCCCATGA CCGTCATCTC GGAGGGCGAA GGGGAAGCCA CGGAGCCCAT AGACAGCACG
    GCTCTTCGGG CCCCGGGTGC TACAAGGGGG TGTTCGCTGG ATGGACTGGC AGGGGGTGGT
    GTGGATGAGG GCAGCCTGCT CTGTGCCGGG GGACCCGCTG CCCCGCTGGA GGCCCAGGCT
    CCTGGTCCGG CAGTGGGGCT GCCCAGCGCA GAGGGCAGGG GCCCTTCAGA ACAGCGAGGC
    GGTCTCGCCG GGGATAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.