CGNL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CGNL1
Entrez Gene ID	517509
Full Name	cingulin like 1
General protein information	Preferred Names cingulin like 1 Names cingulin-like protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010811981.2	XP_010810283.2	cingulin-like protein 1
XM_015465081.1	XP_015320567.1	cingulin-like protein 1
XM_010809305.3	XP_010807607.1	cingulin-like protein 1
XM_010809305.2	XP_010807607.1	cingulin-like protein 1
XM_003586570.5	XP_003586618.1	cingulin-like protein 1
XM_003586570.4	XP_003586618.1	cingulin-like protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CGNL1	NP_116255.2
Danio rerio (zebrafish)	cgnl1	XP_003199047.1
Gallus gallus (chicken)	CGNL1	XP_413790.4
	CGNL1	XP_001092704.1
Mus musculus (house mouse)	Cgnl1	NP_080875.3
Pan troglodytes (chimpanzee)	CGNL1	XP_510437.2
Bos taurus (cattle)	CGNL1	XP_005211807.1
Xenopus tropicalis (tropical clawed frog)	cgnl1	NP_001120551.1
Canis lupus familiaris (dog)	CGNL1	XP_005638512.1
Rattus norvegicus (Norway rat)	Cgnl1	NP_001101634.1

Gene Ontology

The following CGNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CGNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219862	XM_010809305.3 Latest version!	Bos taurus cingulin like 1 (CGNL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa219862	XM_003586570.5 Latest version!	Bos taurus cingulin like 1 (CGNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa23296	XM_003586570.4 Latest version!	Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa23296	XM_010809305.2 Latest version!	Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa23296	XM_010811981.2 Latest version!	Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa23296	XM_015465081.1 Latest version!	Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CGNL1 ( XM_010809305.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807607.1 Bos taurus cingulin like 1 (CGNL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219862
Clone ID Related Accession (Same CDS sequence)	XM_010809305.3 , XM_003586570.5
Accession Version	XM_010809305.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TTATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807607.1
CDS	177..4058
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_010809305.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin like 1 (CGNL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809305.3

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TTATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGNL1 ( XM_003586570.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586618.1 Bos taurus cingulin like 1 (CGNL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219862
Clone ID Related Accession (Same CDS sequence)	XM_010809305.3 , XM_003586570.5
Accession Version	XM_003586570.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586570.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TTATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586618.1
CDS	99..3980
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_003586570.5
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin like 1 (CGNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586570.5

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TTATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGNL1 ( XM_010811981.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810283.2 Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.

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CloneID	OBa23296
Clone ID Related Accession (Same CDS sequence)	XM_010811981.2 , XM_015465081.1 , XM_010809305.2 , XM_003586570.4
Accession Version	XM_010811981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810283.2
CDS	114..3995
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_010811981.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811981.2

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGNL1 ( XM_015465081.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320567.1 Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23296
Clone ID Related Accession (Same CDS sequence)	XM_010811981.2 , XM_015465081.1 , XM_010809305.2 , XM_003586570.4
Accession Version	XM_015465081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320567.1
CDS	172..4053
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_015465081.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465081.1

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGNL1 ( XM_010809305.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807607.1 Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23296
Clone ID Related Accession (Same CDS sequence)	XM_010811981.2 , XM_015465081.1 , XM_010809305.2 , XM_003586570.4
Accession Version	XM_010809305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807607.1
CDS	172..4053
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_010809305.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin-like 1 (CGNL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809305.2

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGNL1 ( XM_003586570.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586618.1 Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.

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CloneID	OBa23296
Clone ID Related Accession (Same CDS sequence)	XM_010811981.2 , XM_015465081.1 , XM_010809305.2 , XM_003586570.4
Accession Version	XM_003586570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cingulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586570.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586618.1
CDS	114..3995
Translation	MELYFGEYQHVQQEYGVHLRLASDETRKSRNSQHAKAGSYGVSIRVQGIDGHPYIVLNNTEQCLAGTSFSENGPSFPAPMINNLPLHPSKGSVVEESSAEELQLPENPYAPPGPVRNLKQPSVFEGKNGALERKEEPRKASPMLNFERHPELLQPYDPEKNELSLKNHQPPETSWLKALREGTNNKKAWTCMPKPSLSQPTSPPSEDPARSNVTAIRLCSSVVIDDPKKQTSVCMNVQSCSKECLAEEALAPSGRPLPTPSLQAHLEAKKTRPDVLPFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRYIPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGMQRRGRSGKRNRINPEDRKRSRSVDSAFPFGLQGNSEYLTEFSRNLGKSSEHLLRPSQVCPPRAQEHRGRQGGGRAFARLLGAQPRPPLQNKDGKVPENKSAQESPTGRTSSLPAQTKKEEEIKTVTATLMLQNPALATSPDSGTKRISVKTFTSTSNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGSTDNDDATKRKVNLVFEKIQTLKSRAAGSTQGNNQASNSSSEVKDLLEQKNKLTTEVAELQTQLQLEVKNQQNIKEERDRMKADLEVLQSQHDSKVEESTVLQRRLEESEGALRRHLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKQSEDREKGALIEELLQAKQDLQHLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDREMDKLKEQYDAELLALRESVEEATKNVEVLASRSSSAEQTQVGAEMRVKALQEENEKLRGSVEELERTVARLQRQLADLQDDEAKAKETLKKCEGETRQLEEALLHARKEEKEATSAKRALQTELEEAQRNLSRATQEQKQLSERLKDEAEQKEQLRRLKNEMENERWHLDKTIEKLQKEMADIVEVSRTSTLELQNQLDEYKEKNRRELAEMQRQLKEKTLEAEKSHLTAMKLQDEMRLMEEELRDYQRAQEEALTKRQLLEQTLKDLEYELEAKSHLKDDRGRLVKQMEDKVSQLEMELEEERNNSDLLSERITRSREQMEQMRNELLQERAMKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLESEERDRASLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDVNEQLQGQLNSMKKDLRLKKLPSKVLDDVDDDDDLSTDGGSLYEAPLSYTFPKDSTVVSQI

Target ORF information:

RefSeq Version	XM_003586570.4
Organism	Bos taurus(cattle)
Definition	Bos taurus cingulin-like 1 (CGNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586570.4

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGTGA ATATCAACAT GTGCAACAGG AATATGGTGT CCACCTGAGA 
CTCGCAAGTG ATGAAACCAG GAAGTCCAGG AATTCCCAGC ATGCTAAGGC AGGCTCCTAC 
GGTGTCAGTA TTCGGGTCCA GGGCATTGAT GGCCACCCCT ACATAGTCCT CAATAACACA 
GAGCAGTGTC TGGCAGGCAC GTCTTTTTCT GAAAACGGGC CATCATTTCC AGCTCCGATG 
ATAAACAACC TGCCCCTCCA TCCCAGCAAG GGGTCTGTGG TGGAAGAGAG CAGTGCAGAA 
GAACTGCAGC TCCCAGAAAA CCCATATGCC CCACCTGGCC CAGTAAGAAA CCTGAAACAG 
CCCTCTGTCT TTGAGGGCAA GAATGGGGCT CTAGAGCGCA AAGAGGAGCC AAGGAAAGCA 
TCCCCCATGC TGAACTTTGA GAGGCATCCA GAGCTTCTAC AGCCCTATGA TCCTGAAAAG 
AATGAGTTGA GCTTGAAAAA TCACCAGCCT CCTGAAACCA GTTGGCTGAA AGCTTTAAGA 
GAAGGGACGA ACAATAAGAA GGCCTGGACT TGCATGCCCA AACCTAGCCT TTCCCAGCCC 
ACCAGCCCTC CCTCCGAAGA CCCGGCCAGG TCCAATGTGA CGGCCATCCG TCTGTGCAGC 
TCCGTGGTCA TCGATGACCC CAAGAAGCAG ACCTCGGTGT GTATGAACGT TCAGAGCTGC 
AGCAAGGAAT GTCTGGCAGA GGAGGCTCTT GCCCCTAGCG GGAGGCCCCT GCCCACCCCC 
AGCCTGCAAG CCCACCTCGA AGCCAAGAAA ACCAGACCGG ATGTGCTTCC CTTCCGGCGC 
CAGGACTCAG CCGGGCCTGT GCTGGACGGA GCCCGGTCCC GGAGATCCTC CTCGTCATCC 
ACGACTCCCA CTTCAGCCAA CTCTTTGTAC AGATTTTTAC TCGATGATCA AGAATGTGCC 
ATCCACGCCG ACAATGTGAA TCGTCACGAA AACAGAAGGT ACATCCCCTT CTTGCCTGGA 
ACTGGGCGGG ATATTGACAC GGGGTCGATT CCTGGCGTGG ATCAGTTGAT TGAAAAATTT 
GACCAAAAAC CTGGGATGCA GAGAAGAGGC AGGTCTGGGA AGAGAAACAG GATCAACCCA 
GAAGACAGGA AAAGGTCCCG AAGTGTGGAC AGTGCCTTTC CTTTCGGTCT CCAGGGGAAC 
TCGGAGTACC TAACGGAATT CAGCAGGAAC CTGGGCAAGT CCAGCGAACA CCTGCTGCGG 
CCGTCACAGG TGTGCCCGCC GCGTGCGCAG GAGCACCGGG GCAGGCAAGG TGGGGGGCGC 
GCCTTCGCCA GGCTGCTGGG AGCCCAGCCC AGGCCGCCCC TGCAGAACAA AGACGGGAAA 
GTTCCAGAAA ACAAAAGTGC TCAGGAAAGT CCGACTGGGC GCACATCCTC CCTCCCAGCA 
CAGACCAAGA AGGAGGAGGA AATCAAAACA GTCACGGCTA CGCTGATGTT ACAGAATCCG 
GCGCTGGCGA CTTCCCCTGA TTCAGGGACC AAGAGGATTT CCGTGAAGAC GTTTACTTCC 
ACCTCAAATA CTCAGGCCAC ACCGGATCTG TTAAAAGGCC AGCAGGAGCT CACTCAGCAA 
ACCAACGAGG AGACGGCCAA GCAGATACTT TACAATTACC TCAAAGAAGG AAGCACCGAT 
AATGATGATG CTACTAAGAG GAAGGTCAAC CTGGTGTTTG AGAAAATACA GACTTTGAAA 
TCTCGAGCAG CAGGGAGCAC ACAAGGAAAT AATCAAGCTT CTAATTCTTC ATCTGAAGTC 
AAAGATCTCT TGGAACAGAA GAACAAGTTG ACCACAGAAG TGGCTGAACT TCAGACACAG 
CTTCAACTGG AAGTCAAGAA TCAGCAGAAC ATCAAAGAAG AGAGAGACAG GATGAAAGCA 
GACTTAGAAG TGTTGCAAAG CCAGCATGAC AGCAAGGTGG AGGAGAGCAC TGTGCTGCAG 
CGGCGGCTGG AGGAAAGTGA GGGGGCGCTC CGGAGACACC TGGAGGAGCT GTTCCAAGTG 
AAGATGGAAC GGGAGCAGCA CCAGACTGAA ATCAGAGATC TCCAGGATCA GCTCTCGGAA 
ATGCATGATG AGTTGGACAG TGCGAAACAA TCCGAGGACA GGGAGAAGGG GGCTCTGATC 
GAGGAGCTCC TGCAGGCAAA GCAGGACCTT CAGCATCTGC TCATTGCCAA AGAGGAGCAG 
GAAGACCTCT TGAGGAAGCG GGAGCGTGAA CTCACTGCGC TAAAGGGAGC CCTGAAGGAA 
GAGGTTTCCA GCCACGACCG GGAGATGGAC AAGCTGAAGG AGCAGTATGA CGCTGAGCTG 
CTGGCCCTGC GGGAGAGTGT GGAGGAGGCC ACTAAGAACG TCGAGGTCCT GGCCAGTCGG 
AGCAGTAGCG CAGAGCAAAC CCAGGTGGGG GCAGAGATGC GCGTAAAGGC TCTGCAGGAG 
GAGAATGAGA AGCTGCGGGG AAGTGTGGAA GAGCTGGAGC GCACTGTGGC TAGGCTGCAG 
AGGCAGCTTG CTGACCTTCA AGATGACGAG GCCAAAGCAA AGGAGACTCT CAAGAAGTGC 
GAGGGAGAAA CTCGGCAATT AGAAGAGGCC CTTCTGCATG CAAGAAAGGA AGAGAAAGAA 
GCCACGTCAG CCAAAAGAGC CCTGCAGACT GAGCTGGAAG AGGCTCAGAG AAATCTGAGC 
CGGGCCACCC AGGAACAGAA GCAGTTGTCT GAGAGGCTCA AAGACGAGGC CGAGCAGAAG 
GAGCAGTTAA GGAGACTGAA GAATGAAATG GAGAATGAGC GATGGCACCT TGACAAGACC 
ATTGAGAAAC TGCAGAAGGA GATGGCCGAC ATTGTCGAGG TGTCCCGCAC ATCAACGCTG 
GAGCTCCAGA ACCAACTGGA TGAATACAAG GAGAAAAATC GCAGGGAACT TGCGGAGATG 
CAAAGACAGC TGAAGGAGAA AACTCTAGAG GCAGAAAAGT CCCATCTGAC TGCAATGAAG 
TTGCAGGATG AGATGCGCCT GATGGAGGAA GAGTTACGGG ACTACCAGAG AGCTCAGGAA 
GAAGCGCTCA CGAAAAGGCA GCTTCTAGAG CAGACACTCA AGGACCTGGA GTACGAGCTG 
GAGGCGAAGA GTCACCTCAA GGATGACCGA GGCCGACTCG TCAAGCAGAT GGAGGACAAG 
GTGTCTCAAC TGGAGATGGA GCTGGAAGAA GAAAGAAACA ACTCGGATTT GCTGTCTGAG 
AGGATCACCC GGAGCAGGGA ACAGATGGAG CAGATGCGGA ATGAGCTACT TCAGGAGCGA 
GCCATGAAGC AAGACTTGGA GTGTGACAAG ATTTCTCTGG AGAGACAGAA CAAGGACTTA 
AAGAGCCGGA TAATCCACCT GGAAGGTTCC TACAGGTCCA GCAAAGAGGG CCTGGTGGTG 
CAGATGGAGG CCAGGATCGC AGAGCTGGAG GACCGTCTGG AGAGTGAGGA GAGGGACCGG 
GCCAGCCTCC AACTGAGCAA CCGCCGGCTG GAGCGGAAGG TGAAGGAGCT GGTGATGCAG 
GTGGACGATG AGCACCTGTC GCTGACTGAT CAGAAGGACC AGTTGAGCTT GCGCTTGAAG 
GCAATGAAGC GACAAGTAGA GGAGGCTGAG GAGGAAATCG ACAGGCTGGA AAGTTCTAAG 
AAGAAGCTGC AGAGGGAGCT GGAGGAGCAG ATGGACGTGA ATGAGCAGCT GCAGGGCCAG 
CTCAACTCCA TGAAGAAGGA CTTAAGACTT AAGAAGCTGC CAAGCAAAGT GCTGGATGAT 
GTGGACGACG ACGACGACCT CAGCACGGAT GGGGGGAGCC TCTATGAGGC GCCACTGAGC 
TACACCTTCC CCAAGGACAG CACCGTCGTC AGCCAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CGNL1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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