PDHX cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDHX
Entrez Gene ID	517402
Full Name	pyruvate dehydrogenase complex component X
General protein information	Preferred Names pyruvate dehydrogenase complex component X Names pyruvate dehydrogenase protein X component E3-binding protein E3BP dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex proX pyruvate dehydrogenase protein X component-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001075751.1	NP_001069219.1	pyruvate dehydrogenase protein X component precursor
XM_024975273.1	XP_024831041.1	pyruvate dehydrogenase protein X component isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pdhx	NP_780303.1
Gallus gallus (chicken)	PDHX	NP_001026358.1
Xenopus tropicalis (tropical clawed frog)	pdhx	XP_002937341.2
Pan troglodytes (chimpanzee)	PDHX	XP_001149489.1
	PDHX	XP_001109997.2
Bos taurus (cattle)	LOC517402	NP_001069219.1
Homo sapiens (human)	PDHX	NP_003468.2
Canis lupus familiaris (dog)	PDHX	XP_533153.2
Danio rerio (zebrafish)	pdhx	NP_956854.1

Gene Ontology
Bibliography

Related articles in PubMed

Variation in the organization and subunit composition of the mammalian pyruvate dehydrogenase complex E2/E3BP core assembly.
Vijayakrishnan S, Callow P, Nutley MA, McGow DP, Gilbert D, Kropholler P, Cooper A, Byron O, Lindsay JG
The Biochemical journal437(3)565-74(2011 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that the recombinant and bovine E2/E3BP cores bind E3s with a 2:1 stoichiometry, and propose that mammalian PDC comprises a heterogeneous population of assemblies incorporating a network of E3 cross-bridges above the core surface.
Title: Variation in the organization and subunit composition of the mammalian pyruvate dehydrogenase complex E2/E3BP core assembly.

The following PDHX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43750	NM_001075751.1 Latest version!	Bos taurus pyruvate dehydrogenase complex component X (PDHX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa222798	XM_024975273.1 Latest version!	Bos taurus pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDHX ( NM_001075751.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069219.1 Bos taurus pyruvate dehydrogenase complex component X (PDHX), mRNA.

Price & Availability↑

CloneID	OBa43750
Clone ID Related Accession (Same CDS sequence)	NM_001075751.1
Accession Version	NM_001075751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	pyruvate dehydrogenase protein X component precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC120413.1. On Oct 4, 2006 this sequence version replaced XM_595571.2. ##Evidence-Data-START## Transcript exon combination :: BC120413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCCT CCTGGAGGCT AGGCTGTGAC CCGCGGCTGC TGCGTTGCCT TCTGGGCTTT 
GGCAGTCGCA GAAGCCCAGA GCTGGTTAAG GGGGCTGCTA GGTGGTCTGT CGGCCGTGGA 
GCTAGTTGGA GATGGTTTCA CAGTACGCAG TGGCTGCGGG CTGATCCTAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGGAACATTG TGAAATGGCT GAAAAAGGAG 
GGTGAAGCTG TGAGTGCTGG AGATGCCTTA TGTGAAATAG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CGAGTGATGA TGGCATCTTG GCCAAAATAG TGGTGGCAGA AGGATCTAAA 
AATATACGGT TGGGTTCACT GATTGGTTTG CTAGTTGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CTAAAGATAC GGGTCCTCCA CCACCAGCTG CAAAACCATC AGTGCCTCCC 
CCCTCAGCAG AACCACAGAT TGCTACCCCT GTCAAGAAGG AACACCCACC AGGAAAAGTG 
CAGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AGCACGCACT GGATGCTAAC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTGGTC 
CAGTTGAAAC AGACGGGCAA GATCACTGAA CCCAGACCCA CCGCAGCCCT GCCAACCACT 
CCCGCAGCGC CTTTGCCCCC ACAGGCTGCA GCCACAGCAT CTTACCCCCG GCCGATGATC 
CCTCCTGTAT CCACTCCCGG ACAACCTAAT GTAGAGGGCA CATTCACTGA AATCCCAGCT 
AGCAATATTA GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGCAC CATACCTCAT 
GCGTATGCTA CTACTGACTG TGACCTTGGA GCTGTCCTAA CAGCTAGACA GAATCTGGTC 
AGAGATGACA TTAAAGTATC AGTAAACGAT TTTATCATCA AGGCAGCAGC TGTTACACTT 
AAACAAATGC CTAATGTTAA TGCAAGCTGG GACGGAGAGG GTGCAAAGCA ACTGCCCTCT 
ATTGACATTT CAGTGGCAGT GGCAACAGAT AGAGGTCTAA TTACACCAGT CATAAAAGAT 
GCTGCTGCTA AGGGTCTACA GGAGATTGCT GATTCTGTAA AGGCTCTATC AAAGAAAGCC 
AGAGATGGAA AATTGTTACC TGAAGAATAT CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGATGA ATTTACTGCC GTGATCAACC CTCCTCAGGC CTGCATTTTG 
GCGGTAGGGA GATTCCGACC TGTGCTGAAG CTCACTCAGG ATGAAGAGGG GAATGCCCAG 
CTGCAGCAAC GTCAGCTCAT AACGGTCACC ATGTCCAGTG ACAGCCGGGT GGTAGACGAT 
GAACTGGCCA CCAGGTTTCT GGAAAGTTTT AAAGCAAACC TGGAGAATCC TCTCCGACTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069219.1
CDS	19..1524
Translation	MAASWRLGCDPRLLRCLLGFGSRRSPELVKGAARWSVGRGASWRWFHSTQWLRADPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVAEGSKNIRLGSLIGLLVEEGEDWKHVEIPKDTGPPPPAAKPSVPPPSAEPQIATPVKKEHPPGKVQFRLSPAARNILEKHALDANQGTATGPRGIFTKEDALKLVQLKQTGKITEPRPTAALPTTPAAPLPPQAAATASYPRPMIPPVSTPGQPNVEGTFTEIPASNIRRVIAKRLTESKSTIPHAYATTDCDLGAVLTARQNLVRDDIKVSVNDFIIKAAAVTLKQMPNVNASWDGEGAKQLPSIDISVAVATDRGLITPVIKDAAAKGLQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTQDEEGNAQLQQRQLITVTMSSDSRVVDDELATRFLESFKANLENPLRLA

Target ORF information:

RefSeq Version	NM_001075751.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate dehydrogenase complex component X (PDHX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075751.1

ORF Insert Sequence:

ATGGCGGCCT CCTGGAGGCT AGGCTGTGAC CCGCGGCTGC TGCGTTGCCT TCTGGGCTTT 
GGCAGTCGCA GAAGCCCAGA GCTGGTTAAG GGGGCTGCTA GGTGGTCTGT CGGCCGTGGA 
GCTAGTTGGA GATGGTTTCA CAGTACGCAG TGGCTGCGGG CTGATCCTAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGGAACATTG TGAAATGGCT GAAAAAGGAG 
GGTGAAGCTG TGAGTGCTGG AGATGCCTTA TGTGAAATAG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CGAGTGATGA TGGCATCTTG GCCAAAATAG TGGTGGCAGA AGGATCTAAA 
AATATACGGT TGGGTTCACT GATTGGTTTG CTAGTTGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CTAAAGATAC GGGTCCTCCA CCACCAGCTG CAAAACCATC AGTGCCTCCC 
CCCTCAGCAG AACCACAGAT TGCTACCCCT GTCAAGAAGG AACACCCACC AGGAAAAGTG 
CAGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AGCACGCACT GGATGCTAAC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTGGTC 
CAGTTGAAAC AGACGGGCAA GATCACTGAA CCCAGACCCA CCGCAGCCCT GCCAACCACT 
CCCGCAGCGC CTTTGCCCCC ACAGGCTGCA GCCACAGCAT CTTACCCCCG GCCGATGATC 
CCTCCTGTAT CCACTCCCGG ACAACCTAAT GTAGAGGGCA CATTCACTGA AATCCCAGCT 
AGCAATATTA GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGCAC CATACCTCAT 
GCGTATGCTA CTACTGACTG TGACCTTGGA GCTGTCCTAA CAGCTAGACA GAATCTGGTC 
AGAGATGACA TTAAAGTATC AGTAAACGAT TTTATCATCA AGGCAGCAGC TGTTACACTT 
AAACAAATGC CTAATGTTAA TGCAAGCTGG GACGGAGAGG GTGCAAAGCA ACTGCCCTCT 
ATTGACATTT CAGTGGCAGT GGCAACAGAT AGAGGTCTAA TTACACCAGT CATAAAAGAT 
GCTGCTGCTA AGGGTCTACA GGAGATTGCT GATTCTGTAA AGGCTCTATC AAAGAAAGCC 
AGAGATGGAA AATTGTTACC TGAAGAATAT CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGATGA ATTTACTGCC GTGATCAACC CTCCTCAGGC CTGCATTTTG 
GCGGTAGGGA GATTCCGACC TGTGCTGAAG CTCACTCAGG ATGAAGAGGG GAATGCCCAG 
CTGCAGCAAC GTCAGCTCAT AACGGTCACC ATGTCCAGTG ACAGCCGGGT GGTAGACGAT 
GAACTGGCCA CCAGGTTTCT GGAAAGTTTT AAAGCAAACC TGGAGAATCC TCTCCGACTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHX ( XM_024975273.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831041.1 Bos taurus pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222798
Clone ID Related Accession (Same CDS sequence)	XM_024975273.1
Accession Version	XM_024975273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate dehydrogenase protein X component isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CCTGGAGGCT AGGCTGTGAC CCGCGGCTGC TGCGTTGCCT TCTGGGCTTT 
GGCAGTCGCA GAAGCCCAGA GCTGGTTAAG GGGGCTGCTA GGTGGTCTGT CGGCCGTGGA 
GCTAGTTGGA GATGGTTTCA CAGTACGCAG TGGCTGCGGG CTGATCCTAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGGAACATTG TGAAATGGCT GAAAAAGGAG 
GGTGAAGCTG TGAGTGCTGG AGATGCCTTA TGTGAAATAG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CGAGTGATGA TGGCATCTTG GCCAAAATAG TGGTGGCAGA AGGATCTAAA 
AATATACGGT TGGGTTCACT GATTGGTTTG CTAGTTGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CTAAAGATAC GGGTCCTCCA CCACCAGCTG CAAAACCATC AGTGCCTCCC 
CCCTCAGCAG AACCACAGAT TGCTACCCCT GTCAAGAAGG AACACCCACC AGGAAAAGTG 
CAGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AGCACGCACT GGATGCTAAC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTGGTC 
CAGTTGAAAC AGACGGGCAA GATCACTGAA CCCAGACCCA CCGCAGCCCT GCCAACCACT 
CCCGCAGCGC CTTTGCCCCC ACAGGCTGCA GCCACAGCAT CTTACCCCCG GCCGATGATC 
CCTCCTGTAT CCACTCCCGG ACAACCTAAT GTAGAGGGCA CATTCACTGA AATCCCAGCT 
AGCAATATTA GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGCAC CATACCTCAT 
GCGTATGCTA CTACTGACTG TGACCTTGGA GCTGTCCTAA CAGCTAGACA GAATCTGGTC 
AGAGATGACA TTAAAGTATC AGTAAACGAT TTTATCATCA AGGCAGCAGC TGTTACACTT 
AAACAAATGC CTAATGTTAA TGCAAGCTGG GACGGAGAGG GTGCAAAGCA ACTGCCCTCT 
ATTGACATTT CAGTGGCAGT GGCAACAGAT AGAGGTCTAA TTACACCAGT CATAAAAGAT 
GCTGCTGCTA AGGGTCTACA GGAGATTGCT GATTCTGTAA AGGAGAATCC GCATGGACAG 
AGGAGCCTGG TGGTCTACGA TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831041.1
CDS	48..1274
Translation	MAASWRLGCDPRLLRCLLGFGSRRSPELVKGAARWSVGRGASWRWFHSTQWLRADPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVAEGSKNIRLGSLIGLLVEEGEDWKHVEIPKDTGPPPPAAKPSVPPPSAEPQIATPVKKEHPPGKVQFRLSPAARNILEKHALDANQGTATGPRGIFTKEDALKLVQLKQTGKITEPRPTAALPTTPAAPLPPQAAATASYPRPMIPPVSTPGQPNVEGTFTEIPASNIRRVIAKRLTESKSTIPHAYATTDCDLGAVLTARQNLVRDDIKVSVNDFIIKAAAVTLKQMPNVNASWDGEGAKQLPSIDISVAVATDRGLITPVIKDAAAKGLQEIADSVKENPHGQRSLVVYDP

Target ORF information:

RefSeq Version	XM_024975273.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975273.1

ORF Insert Sequence:

ATGGCGGCCT CCTGGAGGCT AGGCTGTGAC CCGCGGCTGC TGCGTTGCCT TCTGGGCTTT 
GGCAGTCGCA GAAGCCCAGA GCTGGTTAAG GGGGCTGCTA GGTGGTCTGT CGGCCGTGGA 
GCTAGTTGGA GATGGTTTCA CAGTACGCAG TGGCTGCGGG CTGATCCTAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGGAACATTG TGAAATGGCT GAAAAAGGAG 
GGTGAAGCTG TGAGTGCTGG AGATGCCTTA TGTGAAATAG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CGAGTGATGA TGGCATCTTG GCCAAAATAG TGGTGGCAGA AGGATCTAAA 
AATATACGGT TGGGTTCACT GATTGGTTTG CTAGTTGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CTAAAGATAC GGGTCCTCCA CCACCAGCTG CAAAACCATC AGTGCCTCCC 
CCCTCAGCAG AACCACAGAT TGCTACCCCT GTCAAGAAGG AACACCCACC AGGAAAAGTG 
CAGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AGCACGCACT GGATGCTAAC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTGGTC 
CAGTTGAAAC AGACGGGCAA GATCACTGAA CCCAGACCCA CCGCAGCCCT GCCAACCACT 
CCCGCAGCGC CTTTGCCCCC ACAGGCTGCA GCCACAGCAT CTTACCCCCG GCCGATGATC 
CCTCCTGTAT CCACTCCCGG ACAACCTAAT GTAGAGGGCA CATTCACTGA AATCCCAGCT 
AGCAATATTA GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGCAC CATACCTCAT 
GCGTATGCTA CTACTGACTG TGACCTTGGA GCTGTCCTAA CAGCTAGACA GAATCTGGTC 
AGAGATGACA TTAAAGTATC AGTAAACGAT TTTATCATCA AGGCAGCAGC TGTTACACTT 
AAACAAATGC CTAATGTTAA TGCAAGCTGG GACGGAGAGG GTGCAAAGCA ACTGCCCTCT 
ATTGACATTT CAGTGGCAGT GGCAACAGAT AGAGGTCTAA TTACACCAGT CATAAAAGAT 
GCTGCTGCTA AGGGTCTACA GGAGATTGCT GATTCTGTAA AGGAGAATCC GCATGGACAG 
AGGAGCCTGG TGGTCTACGA TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variation in the organization and subunit composition of the mammalian pyruvate dehydrogenase complex E2/E3BP core assembly.
The Biochemical journal437(3)565-74(2011 Aug)
Vijayakrishnan S,Callow P,Nutley MA,McGow DP,Gilbert D,Kropholler P,Cooper A,Byron O,Lindsay JG

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PDHX cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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