TNS3 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015463074.1	XP_015318560.1	tensin-3 isoform X6
XM_005198091.3	XP_005198148.2	tensin-3 isoform X6
XM_015463075.1	XP_015318561.1	tensin-3 isoform X6
XM_015470044.1	XP_015325530.1	tensin-3 isoform X6
XM_005205612.4	XP_005205669.2	tensin-3 isoform X3
XM_015463073.1	XP_015318559.1	tensin-3 isoform X1
XM_010827364.2	XP_010825666.2	tensin-3 isoform X5
XM_015470042.1	XP_015325528.1	tensin-3 isoform X1
XM_010804393.2	XP_010802695.2	tensin-3 isoform X2
XM_005205612.3	XP_005205669.1	tensin-3 isoform X6
XM_010827362.2	XP_010825664.2	tensin-3 isoform X2
XM_594710.9	XP_594710.8	tensin-3 isoform X4
XM_010804394.2	XP_010802696.2	tensin-3 isoform X3
XM_005205611.3	XP_005205668.3	tensin-3 isoform X4
XM_015470043.1	XP_015325529.1	tensin-3 isoform X6
XM_024991105.1	XP_024846873.1	tensin-3 isoform X3
XM_024991104.1	XP_024846872.1	tensin-3 isoform X2
XM_010827363.2	XP_010825665.2	tensin-3 isoform X3
XM_010804395.2	XP_010802697.2	tensin-3 isoform X5
XM_005205611.4	XP_005205668.4	tensin-3 isoform X1
XM_015470044.2	XP_015325530.2	tensin-3 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233995	XM_024991104.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa233996	XM_015470044.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233996	XM_024991105.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233996	XM_005205612.4 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89748	XM_010827364.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89748	XM_010804395.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa233994	XM_005205611.4 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa89744	XM_015470042.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89744	XM_015463073.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89745	XM_010827362.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89745	XM_010804393.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89746	XM_010827363.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89746	XM_010804394.2 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89747	XM_594710.9 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89747	XM_005205611.3 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa18699	XM_015463075.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa18699	XM_015463074.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa18699	XM_005198091.3 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa18699	XM_005205612.3 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa18699	XM_015470044.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa18699	XM_015470043.1 Latest version!	Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

TNS3 ( XM_024991104.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846872.1 Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa233995
Clone ID Related Accession (Same CDS sequence)	XM_024991104.1
Accession Version	XM_024991104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGAACCCC GAGGCTGCCC TGAGATTCTC AGTCATTCTG TGGGGATGTC AGAGAGCCCT 
GTTGGACCCA AACCCACGAT GCTCCGGGCC GACATGCCCA CGGCGCCCTC CTTCCAGCGG 
GCATTCACGT CCTCCTGCAC CATTTCTGGG AACAGCCCCA GCCAGAGGAG AGGGAGCCCT 
TCCTCAGCTG AGCACCAGTG GGTGGAGACC AGCCCCAAGT CCACGCTGAC CCTCCTGGGG 
GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG CGACCCCACT TCCCGCCTGC CGACCTCCAG 
ACATCCTTCC ACGGCCCCGA GCTGTCCCTG GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG 
AGCAACCAGG CCTTCCTGAG CTTCAGCACT GCCCCCATGG CGGGAGGTGG GCTCCCCGCC 
GGGGAGGACC CCGGGGCCTT GCTGGCCAAT TCCCACGGAG CAGCCCAGGC CCCTGGCAAC 
CCACTGACGG CAGCTGAGGC TGCCGACAAC AGCTTCCTGT CCCACAGCTT TCTCACGGTG 
GCACCTGGAC ACAGCGGCCA CCACAGCCCG GTCCTGCAGG GCCCGGGACT GGCTCTGCCC 
GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG ACCTCAGAGG GAGATCGGTC TTTTGGCTCC 
GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT CCTCACAGCG GGAGTACCAT GAGCATCCCT 
TTTCCAAACA TCATCCCGGA CTTTTCCAAG GCTGCCGAGG GGGCAGCACC TTCCCCAGAT 
AATCCAGGTG ATAAGCACGT GTCTGTAAAC TTTGTTCAGG ACACATCCAA GTTCTGGTAC 
AAGGCGGACA TCTCCAGAGA GCAAGCCATC GCCATGCTGA AGGACAAGGA ACCCGGGTCA 
TTCATCGTCA GGGACAGCCA CTCCTTCCGA GGTGCCTACG GCCTGGCCAT GAAGGTGGCC 
ACACCCCCGC CCTCTGTCCT GCAGATGAAC AAGAAAGCTG GAGATTTGGC CAATGAACTT 
GTTCGGCATT TTTTGATTGA GTGTACCCCA AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT 
GAACCATATT TCGGGAGCCT GACGGCTTTG GTGTGTCAGC ATTCCATTAC GCCCCTGGCT 
TTGCCCTGCA AACTGCTCAT TCCGGATAGA GATCCATTGG AGGAAATAGC CGAAAATCCT 
CAGCAAACAG CGGCCAACTC TGCGGCCGAG CTGCTGAAGC AAGGGGCAGC CTGCAATGTG 
TGGTACCTGA ACTCCGTGGA GATGGAGTCC CTCACGGGCC ACCAAGCCAT CCAGAAGGCC 
CTGAGCATGA CCCTAGTCCA GGAGCCACCT CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG 
TCGGCCCAGG GCATCACCCT GACGGACAAC CAGAGGAAGC TCTTCTTCCG GAGACACTAC 
CCCGTGAGCA GCGTGATCTT CTGTGCGCTG GACCCACAGG ACAGAAAGTG GGTCACAGAC 
GGCCCTTGCT CGAGAGTCTT TGGATTTGTG GCCCGGAAGC AGGGCAGTGC CACGGACAAC 
GTGTGCCACC TGTTTGCAGA GCATGACCCC GAGCAGCCGG CCAGCGCCAT TGTCAACTTC 
GTGTCAAAGG TCATGATCGG CTCCCCTAAG AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846872.1
CDS	265..4203
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNQAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_024991104.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991104.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGAACCCC GAGGCTGCCC TGAGATTCTC AGTCATTCTG TGGGGATGTC AGAGAGCCCT 
GTTGGACCCA AACCCACGAT GCTCCGGGCC GACATGCCCA CGGCGCCCTC CTTCCAGCGG 
GCATTCACGT CCTCCTGCAC CATTTCTGGG AACAGCCCCA GCCAGAGGAG AGGGAGCCCT 
TCCTCAGCTG AGCACCAGTG GGTGGAGACC AGCCCCAAGT CCACGCTGAC CCTCCTGGGG 
GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG CGACCCCACT TCCCGCCTGC CGACCTCCAG 
ACATCCTTCC ACGGCCCCGA GCTGTCCCTG GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG 
AGCAACCAGG CCTTCCTGAG CTTCAGCACT GCCCCCATGG CGGGAGGTGG GCTCCCCGCC 
GGGGAGGACC CCGGGGCCTT GCTGGCCAAT TCCCACGGAG CAGCCCAGGC CCCTGGCAAC 
CCACTGACGG CAGCTGAGGC TGCCGACAAC AGCTTCCTGT CCCACAGCTT TCTCACGGTG 
GCACCTGGAC ACAGCGGCCA CCACAGCCCG GTCCTGCAGG GCCCGGGACT GGCTCTGCCC 
GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG ACCTCAGAGG GAGATCGGTC TTTTGGCTCC 
GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT CCTCACAGCG GGAGTACCAT GAGCATCCCT 
TTTCCAAACA TCATCCCGGA CTTTTCCAAG GCTGCCGAGG GGGCAGCACC TTCCCCAGAT 
AATCCAGGTG ATAAGCACGT GTCTGTAAAC TTTGTTCAGG ACACATCCAA GTTCTGGTAC 
AAGGCGGACA TCTCCAGAGA GCAAGCCATC GCCATGCTGA AGGACAAGGA ACCCGGGTCA 
TTCATCGTCA GGGACAGCCA CTCCTTCCGA GGTGCCTACG GCCTGGCCAT GAAGGTGGCC 
ACACCCCCGC CCTCTGTCCT GCAGATGAAC AAGAAAGCTG GAGATTTGGC CAATGAACTT 
GTTCGGCATT TTTTGATTGA GTGTACCCCA AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT 
GAACCATATT TCGGGAGCCT GACGGCTTTG GTGTGTCAGC ATTCCATTAC GCCCCTGGCT 
TTGCCCTGCA AACTGCTCAT TCCGGATAGA GATCCATTGG AGGAAATAGC CGAAAATCCT 
CAGCAAACAG CGGCCAACTC TGCGGCCGAG CTGCTGAAGC AAGGGGCAGC CTGCAATGTG 
TGGTACCTGA ACTCCGTGGA GATGGAGTCC CTCACGGGCC ACCAAGCCAT CCAGAAGGCC 
CTGAGCATGA CCCTAGTCCA GGAGCCACCT CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG 
TCGGCCCAGG GCATCACCCT GACGGACAAC CAGAGGAAGC TCTTCTTCCG GAGACACTAC 
CCCGTGAGCA GCGTGATCTT CTGTGCGCTG GACCCACAGG ACAGAAAGTG GGTCACAGAC 
GGCCCTTGCT CGAGAGTCTT TGGATTTGTG GCCCGGAAGC AGGGCAGTGC CACGGACAAC 
GTGTGCCACC TGTTTGCAGA GCATGACCCC GAGCAGCCGG CCAGCGCCAT TGTCAACTTC 
GTGTCAAAGG TCATGATCGG CTCCCCTAAG AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_005205612.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205669.2 Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa233996
Clone ID Related Accession (Same CDS sequence)	XM_005205612.4 , XM_024991105.1 , XM_015470044.2
Accession Version	XM_005205612.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205669.2
CDS	365..4678
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFQKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNQAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_005205612.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205612.4

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_024991105.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846873.1 Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

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CloneID	OBa233996
Clone ID Related Accession (Same CDS sequence)	XM_005205612.4 , XM_024991105.1 , XM_015470044.2
Accession Version	XM_024991105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846873.1
CDS	282..4595
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFQKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNQAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_024991105.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991105.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015470044.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325530.2 Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

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CloneID	OBa233996
Clone ID Related Accession (Same CDS sequence)	XM_005205612.4 , XM_024991105.1 , XM_015470044.2
Accession Version	XM_015470044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325530.2
CDS	211..4524
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFQKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNQAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015470044.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470044.2

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT CGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAC TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CAGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTTTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCAGGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAACCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010827364.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825666.2 Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa89748
Clone ID Related Accession (Same CDS sequence)	XM_010827364.2 , XM_010804395.2
Accession Version	XM_010827364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGAA 
CCCCGAGGCT GCCCTGAGAT TCTCAGTCAT TCTGTGGGGA TGTCAGAGAG CCCTGTTGGA 
CCCAAACCCA CGATGCTCCG GGCCGACATG CCCACGGCGC CCTCCTTCCA GCGGGCATTC 
ACGTCCTCCT GCACCATTTC TGGGAACAGC CCCAGCCAGA GGAGAGGGAG CCCTTCCTCA 
GCTGAGCACC AGTGGGTGGA GACCAGCCCC AAGTCCACGC TGACCCTCCT GGGGGGCAGC 
CGGCCTGGCA AGGACAGCCC CGCGCGACCC CACTTCCCGC CTGCCGACCT CCAGACATCC 
TTCCACGGCC CCGAGCTGTC CCTGGCGGAG CCGCCGGAAG CTCTGGGTCC CCCGAGCAAC 
CACGCCTTCC TGAGCTTCAG CACTGCCCCC ATGGCGGGAG GTGGGCTCCC CGCCGGGGAG 
GACCCCGGGG CCTTGCTGGC CAATTCCCAC GGAGCAGCCC AGGCCCCTGG CAACCCACTG 
ACGGCAGCTG AGGCTGCCGA CAACAGCTTC CTGTCCCACA GCTTTCTCAC GGTGGCACCT 
GGACACAGCG GCCACCACAG CCCGGTCCTG CAGGGCCCGG GACTGGCTCT GCCCGGGCAG 
CCACCCCTTC CTGAGAAGAA GCGGACCTCA GAGGGAGATC GGTCTTTTGG CTCCGTCTCG 
CCATCCTCCA GTGGCTTCTC CAGTCCTCAC AGCGGGAGTA CCATGAGCAT CCCTTTTCCA 
AACATCATCC CGGACTTTTC CAAGGCTGCC GAGGGGGCAG CACCTTCCCC AGATAATCCA 
GGTGATAAGC ACGTGTCTGT AAACTTTGTT CAGGACACAT CCAAGTTCTG GTACAAGGCG 
GACATCTCCA GAGAGCAAGC CATCGCCATG CTGAAGGACA AGGAGCCCGG GTCATTCATC 
GTCAGGGACA GCCACTCCTT CCGAGGTGCC TACGGCCTGG CCATGAAGGT GGCCACACCC 
CCGCCCTCTG TCCTGCAGAT GAACAAGAAA GCTGGAGATT TGGCCAATGA ACTTGTTCGG 
CATTTTTTGA TTGAGTGTAC CCCAAAAGGA GTGCGGTTGA AAGGGTGCTC CAATGAACCA 
TATTTCGGGA GCCTGACGGC TTTGGTGTGT CAGCATTCCA TTACGCCCCT GGCTTTGCCC 
TGCAAACTGC TCATTCCGGA TAGAGATCCA TTGGAGGAAA TAGCCGAAAA TCCTCAGCAA 
ACAGCGGCCA ACTCTGCGGC CGAGCTGCTG AAGCAAGGGG CAGCCTGCAA TGTGTGGTAC 
CTGAACTCCG TGGAGATGGA GTCCCTCACG GGCCACCAAG CCATCCAGAA GGCCCTGAGC 
ATGACCCTAG TCCAGGAGCC ACCTCCCTTG TCCACAGTCG TGCACTTCAA GGTGTCGGCC 
CAGGGCATCA CCCTGACGGA CAACCAGAGG AAGCTCTTCT TCCGGAGACA CTACCCCGTG 
AGCAGCGTGA TCTTCTGTGC GCTGGACCCA CAGGACAGAA AGTGGGTCAC AGACGGCCCT 
TGCTCGAGAG TCTTTGGATT TGTGGCCCGG AAGCAGGGCA GTGCCACGGA CAACGTGTGC 
CACCTGTTTG CAGAGCATGA CCCCGAGCAG CCGGCCAGCG CCATTGTCAA CTTCGTGTCA 
AAGGTCATGA TCGGCTCCCC TAAGAAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825666.2
CDS	2182..6594
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010827364.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827364.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGAA 
CCCCGAGGCT GCCCTGAGAT TCTCAGTCAT TCTGTGGGGA TGTCAGAGAG CCCTGTTGGA 
CCCAAACCCA CGATGCTCCG GGCCGACATG CCCACGGCGC CCTCCTTCCA GCGGGCATTC 
ACGTCCTCCT GCACCATTTC TGGGAACAGC CCCAGCCAGA GGAGAGGGAG CCCTTCCTCA 
GCTGAGCACC AGTGGGTGGA GACCAGCCCC AAGTCCACGC TGACCCTCCT GGGGGGCAGC 
CGGCCTGGCA AGGACAGCCC CGCGCGACCC CACTTCCCGC CTGCCGACCT CCAGACATCC 
TTCCACGGCC CCGAGCTGTC CCTGGCGGAG CCGCCGGAAG CTCTGGGTCC CCCGAGCAAC 
CACGCCTTCC TGAGCTTCAG CACTGCCCCC ATGGCGGGAG GTGGGCTCCC CGCCGGGGAG 
GACCCCGGGG CCTTGCTGGC CAATTCCCAC GGAGCAGCCC AGGCCCCTGG CAACCCACTG 
ACGGCAGCTG AGGCTGCCGA CAACAGCTTC CTGTCCCACA GCTTTCTCAC GGTGGCACCT 
GGACACAGCG GCCACCACAG CCCGGTCCTG CAGGGCCCGG GACTGGCTCT GCCCGGGCAG 
CCACCCCTTC CTGAGAAGAA GCGGACCTCA GAGGGAGATC GGTCTTTTGG CTCCGTCTCG 
CCATCCTCCA GTGGCTTCTC CAGTCCTCAC AGCGGGAGTA CCATGAGCAT CCCTTTTCCA 
AACATCATCC CGGACTTTTC CAAGGCTGCC GAGGGGGCAG CACCTTCCCC AGATAATCCA 
GGTGATAAGC ACGTGTCTGT AAACTTTGTT CAGGACACAT CCAAGTTCTG GTACAAGGCG 
GACATCTCCA GAGAGCAAGC CATCGCCATG CTGAAGGACA AGGAGCCCGG GTCATTCATC 
GTCAGGGACA GCCACTCCTT CCGAGGTGCC TACGGCCTGG CCATGAAGGT GGCCACACCC 
CCGCCCTCTG TCCTGCAGAT GAACAAGAAA GCTGGAGATT TGGCCAATGA ACTTGTTCGG 
CATTTTTTGA TTGAGTGTAC CCCAAAAGGA GTGCGGTTGA AAGGGTGCTC CAATGAACCA 
TATTTCGGGA GCCTGACGGC TTTGGTGTGT CAGCATTCCA TTACGCCCCT GGCTTTGCCC 
TGCAAACTGC TCATTCCGGA TAGAGATCCA TTGGAGGAAA TAGCCGAAAA TCCTCAGCAA 
ACAGCGGCCA ACTCTGCGGC CGAGCTGCTG AAGCAAGGGG CAGCCTGCAA TGTGTGGTAC 
CTGAACTCCG TGGAGATGGA GTCCCTCACG GGCCACCAAG CCATCCAGAA GGCCCTGAGC 
ATGACCCTAG TCCAGGAGCC ACCTCCCTTG TCCACAGTCG TGCACTTCAA GGTGTCGGCC 
CAGGGCATCA CCCTGACGGA CAACCAGAGG AAGCTCTTCT TCCGGAGACA CTACCCCGTG 
AGCAGCGTGA TCTTCTGTGC GCTGGACCCA CAGGACAGAA AGTGGGTCAC AGACGGCCCT 
TGCTCGAGAG TCTTTGGATT TGTGGCCCGG AAGCAGGGCA GTGCCACGGA CAACGTGTGC 
CACCTGTTTG CAGAGCATGA CCCCGAGCAG CCGGCCAGCG CCATTGTCAA CTTCGTGTCA 
AAGGTCATGA TCGGCTCCCC TAAGAAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010804395.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802697.2 Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa89748
Clone ID Related Accession (Same CDS sequence)	XM_010827364.2 , XM_010804395.2
Accession Version	XM_010804395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGAA 
CCCCGAGGCT GCCCTGAGAT TCTCAGTCAT TCTGTGGGGA TGTCAGAGAG CCCTGTTGGA 
CCCAAACCCA CGATGCTCCG GGCCGACATG CCCACGGCGC CCTCCTTCCA GCGGGCATTC 
ACGTCCTCCT GCACCATTTC TGGGAACAGC CCCAGCCAGA GGAGAGGGAG CCCTTCCTCA 
GCTGAGCACC AGTGGGTGGA GACCAGCCCC AAGTCCACGC TGACCCTCCT GGGGGGCAGC 
CGGCCTGGCA AGGACAGCCC CGCGCGACCC CACTTCCCGC CTGCCGACCT CCAGACATCC 
TTCCACGGCC CCGAGCTGTC CCTGGCGGAG CCGCCGGAAG CTCTGGGTCC CCCGAGCAAC 
CACGCCTTCC TGAGCTTCAG CACTGCCCCC ATGGCGGGAG GTGGGCTCCC CGCCGGGGAG 
GACCCCGGGG CCTTGCTGGC CAATTCCCAC GGAGCAGCCC AGGCCCCTGG CAACCCACTG 
ACGGCAGCTG AGGCTGCCGA CAACAGCTTC CTGTCCCACA GCTTTCTCAC GGTGGCACCT 
GGACACAGCG GCCACCACAG CCCGGTCCTG CAGGGCCCGG GACTGGCTCT GCCCGGGCAG 
CCACCCCTTC CTGAGAAGAA GCGGACCTCA GAGGGAGATC GGTCTTTTGG CTCCGTCTCG 
CCATCCTCCA GTGGCTTCTC CAGTCCTCAC AGCGGGAGTA CCATGAGCAT CCCTTTTCCA 
AACATCATCC CGGACTTTTC CAAGGCTGCC GAGGGGGCAG CACCTTCCCC AGATAATCCA 
GGTGATAAGC ACGTGTCTGT AAACTTTGTT CAGGACACAT CCAAGTTCTG GTACAAGGCG 
GACATCTCCA GAGAGCAAGC CATCGCCATG CTGAAGGACA AGGAGCCCGG GTCATTCATC 
GTCAGGGACA GCCACTCCTT CCGAGGTGCC TACGGCCTGG CCATGAAGGT GGCCACACCC 
CCGCCCTCTG TCCTGCAGAT GAACAAGAAA GCTGGAGATT TGGCCAATGA ACTTGTTCGG 
CATTTTTTGA TTGAGTGTAC CCCAAAAGGA GTGCGGTTGA AAGGGTGCTC CAATGAACCA 
TATTTCGGGA GCCTGACGGC TTTGGTGTGT CAGCATTCCA TTACGCCCCT GGCTTTGCCC 
TGCAAACTGC TCATTCCGGA TAGAGATCCA TTGGAGGAAA TAGCCGAAAA TCCTCAGCAA 
ACAGCGGCCA ACTCTGCGGC CGAGCTGCTG AAGCAAGGGG CAGCCTGCAA TGTGTGGTAC 
CTGAACTCCG TGGAGATGGA GTCCCTCACG GGCCACCAAG CCATCCAGAA GGCCCTGAGC 
ATGACCCTAG TCCAGGAGCC ACCTCCCTTG TCCACAGTCG TGCACTTCAA GGTGTCGGCC 
CAGGGCATCA CCCTGACGGA CAACCAGAGG AAGCTCTTCT TCCGGAGACA CTACCCCGTG 
AGCAGCGTGA TCTTCTGTGC GCTGGACCCA CAGGACAGAA AGTGGGTCAC AGACGGCCCT 
TGCTCGAGAG TCTTTGGATT TGTGGCCCGG AAGCAGGGCA GTGCCACGGA CAACGTGTGC 
CACCTGTTTG CAGAGCATGA CCCCGAGCAG CCGGCCAGCG CCATTGTCAA CTTCGTGTCA 
AAGGTCATGA TCGGCTCCCC TAAGAAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802697.2
CDS	2182..6594
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010804395.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804395.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGAA 
CCCCGAGGCT GCCCTGAGAT TCTCAGTCAT TCTGTGGGGA TGTCAGAGAG CCCTGTTGGA 
CCCAAACCCA CGATGCTCCG GGCCGACATG CCCACGGCGC CCTCCTTCCA GCGGGCATTC 
ACGTCCTCCT GCACCATTTC TGGGAACAGC CCCAGCCAGA GGAGAGGGAG CCCTTCCTCA 
GCTGAGCACC AGTGGGTGGA GACCAGCCCC AAGTCCACGC TGACCCTCCT GGGGGGCAGC 
CGGCCTGGCA AGGACAGCCC CGCGCGACCC CACTTCCCGC CTGCCGACCT CCAGACATCC 
TTCCACGGCC CCGAGCTGTC CCTGGCGGAG CCGCCGGAAG CTCTGGGTCC CCCGAGCAAC 
CACGCCTTCC TGAGCTTCAG CACTGCCCCC ATGGCGGGAG GTGGGCTCCC CGCCGGGGAG 
GACCCCGGGG CCTTGCTGGC CAATTCCCAC GGAGCAGCCC AGGCCCCTGG CAACCCACTG 
ACGGCAGCTG AGGCTGCCGA CAACAGCTTC CTGTCCCACA GCTTTCTCAC GGTGGCACCT 
GGACACAGCG GCCACCACAG CCCGGTCCTG CAGGGCCCGG GACTGGCTCT GCCCGGGCAG 
CCACCCCTTC CTGAGAAGAA GCGGACCTCA GAGGGAGATC GGTCTTTTGG CTCCGTCTCG 
CCATCCTCCA GTGGCTTCTC CAGTCCTCAC AGCGGGAGTA CCATGAGCAT CCCTTTTCCA 
AACATCATCC CGGACTTTTC CAAGGCTGCC GAGGGGGCAG CACCTTCCCC AGATAATCCA 
GGTGATAAGC ACGTGTCTGT AAACTTTGTT CAGGACACAT CCAAGTTCTG GTACAAGGCG 
GACATCTCCA GAGAGCAAGC CATCGCCATG CTGAAGGACA AGGAGCCCGG GTCATTCATC 
GTCAGGGACA GCCACTCCTT CCGAGGTGCC TACGGCCTGG CCATGAAGGT GGCCACACCC 
CCGCCCTCTG TCCTGCAGAT GAACAAGAAA GCTGGAGATT TGGCCAATGA ACTTGTTCGG 
CATTTTTTGA TTGAGTGTAC CCCAAAAGGA GTGCGGTTGA AAGGGTGCTC CAATGAACCA 
TATTTCGGGA GCCTGACGGC TTTGGTGTGT CAGCATTCCA TTACGCCCCT GGCTTTGCCC 
TGCAAACTGC TCATTCCGGA TAGAGATCCA TTGGAGGAAA TAGCCGAAAA TCCTCAGCAA 
ACAGCGGCCA ACTCTGCGGC CGAGCTGCTG AAGCAAGGGG CAGCCTGCAA TGTGTGGTAC 
CTGAACTCCG TGGAGATGGA GTCCCTCACG GGCCACCAAG CCATCCAGAA GGCCCTGAGC 
ATGACCCTAG TCCAGGAGCC ACCTCCCTTG TCCACAGTCG TGCACTTCAA GGTGTCGGCC 
CAGGGCATCA CCCTGACGGA CAACCAGAGG AAGCTCTTCT TCCGGAGACA CTACCCCGTG 
AGCAGCGTGA TCTTCTGTGC GCTGGACCCA CAGGACAGAA AGTGGGTCAC AGACGGCCCT 
TGCTCGAGAG TCTTTGGATT TGTGGCCCGG AAGCAGGGCA GTGCCACGGA CAACGTGTGC 
CACCTGTTTG CAGAGCATGA CCCCGAGCAG CCGGCCAGCG CCATTGTCAA CTTCGTGTCA 
AAGGTCATGA TCGGCTCCCC TAAGAAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_005205611.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205668.4 Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

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CloneID	OBa233994
Clone ID Related Accession (Same CDS sequence)	XM_005205611.4
Accession Version	XM_005205611.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205611.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG GCCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATCGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ACTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCAGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTTT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCAGGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAACC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205668.4
CDS	12..4547
Translation	MQRSFCSFLRDLFTAPVSGLVSMAGSSTQVRRLWEAGGREVSSGPAKFLAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFQKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNQAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_005205611.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205611.4

ORF Insert Sequence:

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG GCCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATCGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ACTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCAGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTTT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCAGGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAACC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015463073.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318559.1 Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

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CloneID	OBa89744
Clone ID Related Accession (Same CDS sequence)	XM_015463073.1 , XM_015470042.1
Accession Version	XM_015463073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGG GACAACAAGC 
CCACGTTACT CTCCGTTCAG CCCATCCGTG CCACAGATGG GCAGCCCCAG TGCTCTGTAT 
AAAGGAGCTC ATGAACCCCG AGGCTGCCCT GAGATTCTCA GTCATTCTGT GGGGATGTCA 
GAGAGCCCTG TTGGACCCAA ACCCACGATG CTCCGGGCCG ACATGCCCAC GGCGCCCTCC 
TTCCAGCGGG CATTCACGTC CTCCTGCACC ATTTCTGGGA ACAGCCCCAG CCAGAGGAGA 
GGGAGCCCTT CCTCAGCTGA GCACCAGTGG GTGGAGACCA GCCCCAAGTC CACGCTGACC 
CTCCTGGGGG GCAGCCGGCC TGGCAAGGAC AGCCCCGCGC GACCCCACTT CCCGCCTGCC 
GACCTCCAGA CATCCTTCCA CGGCCCCGAG CTGTCCCTGG CGGAGCCGCC GGAAGCTCTG 
GGTCCCCCGA GCAACCACGC CTTCCTGAGC TTCAGCACTG CCCCCATGGC GGGAGGTGGG 
CTCCCCGCCG GGGAGGACCC CGGGGCCTTG CTGGCCAATT CCCACGGAGC AGCCCAGGCC 
CCTGGCAACC CACTGACGGC AGCTGAGGCT GCCGACAACA GCTTCCTGTC CCACAGCTTT 
CTCACGGTGG CACCTGGACA CAGCGGCCAC CACAGCCCGG TCCTGCAGGG CCCGGGACTG 
GCTCTGCCCG GGCAGCCACC CCTTCCTGAG AAGAAGCGGA CCTCAGAGGG AGATCGGTCT 
TTTGGCTCCG TCTCGCCATC CTCCAGTGGC TTCTCCAGTC CTCACAGCGG GAGTACCATG 
AGCATCCCTT TTCCAAACAT CATCCCGGAC TTTTCCAAGG CTGCCGAGGG GGCAGCACCT 
TCCCCAGATA ATCCAGGTGA TAAGCACGTG TCTGTAAACT TTGTTCAGGA CACATCCAAG 
TTCTGGTACA AGGCGGACAT CTCCAGAGAG CAAGCCATCG CCATGCTGAA GGACAAGGAG 
CCCGGGTCAT TCATCGTCAG GGACAGCCAC TCCTTCCGAG GTGCCTACGG CCTGGCCATG 
AAGGTGGCCA CACCCCCGCC CTCTGTCCTG CAGATGAACA AGAAAGCTGG AGATTTGGCC 
AATGAACTTG TTCGGCATTT TTTGATTGAG TGTACCCCAA AAGGAGTGCG GTTGAAAGGG 
TGCTCCAATG AACCATATTT CGGGAGCCTG ACGGCTTTGG TGTGTCAGCA TTCCATTACG 
CCCCTGGCTT TGCCCTGCAA ACTGCTCATT CCGGATAGAG ATCCATTGGA GGAAATAGCC 
GAAAATCCTC AGCAAACAGC GGCCAACTCT GCGGCCGAGC TGCTGAAGCA AGGGGCAGCC 
TGCAATGTGT GGTACCTGAA CTCCGTGGAG ATGGAGTCCC TCACGGGCCA CCAAGCCATC 
CAGAAGGCCC TGAGCATGAC CCTAGTCCAG GAGCCACCTC CCTTGTCCAC AGTCGTGCAC 
TTCAAGGTGT CGGCCCAGGG CATCACCCTG ACGGACAACC AGAGGAAGCT CTTCTTCCGG 
AGACACTACC CCGTGAGCAG CGTGATCTTC TGTGCGCTGG ACCCACAGGA CAGAAAGTGG 
GTCACAGACG GCCCTTGCTC GAGAGTCTTT GGATTTGTGG CCCGGAAGCA GGGCAGTGCC 
ACGGACAACG TGTGCCACCT GTTTGCAGAG CATGACCCCG AGCAGCCGGC CAGCGCCATT 
GTCAACTTCG TGTCAAAGGT CATGATCGGC TCCCCTAAGA AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318559.1
CDS	547..5334
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015463073.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463073.1

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGG GACAACAAGC 
CCACGTTACT CTCCGTTCAG CCCATCCGTG CCACAGATGG GCAGCCCCAG TGCTCTGTAT 
AAAGGAGCTC ATGAACCCCG AGGCTGCCCT GAGATTCTCA GTCATTCTGT GGGGATGTCA 
GAGAGCCCTG TTGGACCCAA ACCCACGATG CTCCGGGCCG ACATGCCCAC GGCGCCCTCC 
TTCCAGCGGG CATTCACGTC CTCCTGCACC ATTTCTGGGA ACAGCCCCAG CCAGAGGAGA 
GGGAGCCCTT CCTCAGCTGA GCACCAGTGG GTGGAGACCA GCCCCAAGTC CACGCTGACC 
CTCCTGGGGG GCAGCCGGCC TGGCAAGGAC AGCCCCGCGC GACCCCACTT CCCGCCTGCC 
GACCTCCAGA CATCCTTCCA CGGCCCCGAG CTGTCCCTGG CGGAGCCGCC GGAAGCTCTG 
GGTCCCCCGA GCAACCACGC CTTCCTGAGC TTCAGCACTG CCCCCATGGC GGGAGGTGGG 
CTCCCCGCCG GGGAGGACCC CGGGGCCTTG CTGGCCAATT CCCACGGAGC AGCCCAGGCC 
CCTGGCAACC CACTGACGGC AGCTGAGGCT GCCGACAACA GCTTCCTGTC CCACAGCTTT 
CTCACGGTGG CACCTGGACA CAGCGGCCAC CACAGCCCGG TCCTGCAGGG CCCGGGACTG 
GCTCTGCCCG GGCAGCCACC CCTTCCTGAG AAGAAGCGGA CCTCAGAGGG AGATCGGTCT 
TTTGGCTCCG TCTCGCCATC CTCCAGTGGC TTCTCCAGTC CTCACAGCGG GAGTACCATG 
AGCATCCCTT TTCCAAACAT CATCCCGGAC TTTTCCAAGG CTGCCGAGGG GGCAGCACCT 
TCCCCAGATA ATCCAGGTGA TAAGCACGTG TCTGTAAACT TTGTTCAGGA CACATCCAAG 
TTCTGGTACA AGGCGGACAT CTCCAGAGAG CAAGCCATCG CCATGCTGAA GGACAAGGAG 
CCCGGGTCAT TCATCGTCAG GGACAGCCAC TCCTTCCGAG GTGCCTACGG CCTGGCCATG 
AAGGTGGCCA CACCCCCGCC CTCTGTCCTG CAGATGAACA AGAAAGCTGG AGATTTGGCC 
AATGAACTTG TTCGGCATTT TTTGATTGAG TGTACCCCAA AAGGAGTGCG GTTGAAAGGG 
TGCTCCAATG AACCATATTT CGGGAGCCTG ACGGCTTTGG TGTGTCAGCA TTCCATTACG 
CCCCTGGCTT TGCCCTGCAA ACTGCTCATT CCGGATAGAG ATCCATTGGA GGAAATAGCC 
GAAAATCCTC AGCAAACAGC GGCCAACTCT GCGGCCGAGC TGCTGAAGCA AGGGGCAGCC 
TGCAATGTGT GGTACCTGAA CTCCGTGGAG ATGGAGTCCC TCACGGGCCA CCAAGCCATC 
CAGAAGGCCC TGAGCATGAC CCTAGTCCAG GAGCCACCTC CCTTGTCCAC AGTCGTGCAC 
TTCAAGGTGT CGGCCCAGGG CATCACCCTG ACGGACAACC AGAGGAAGCT CTTCTTCCGG 
AGACACTACC CCGTGAGCAG CGTGATCTTC TGTGCGCTGG ACCCACAGGA CAGAAAGTGG 
GTCACAGACG GCCCTTGCTC GAGAGTCTTT GGATTTGTGG CCCGGAAGCA GGGCAGTGCC 
ACGGACAACG TGTGCCACCT GTTTGCAGAG CATGACCCCG AGCAGCCGGC CAGCGCCATT 
GTCAACTTCG TGTCAAAGGT CATGATCGGC TCCCCTAAGA AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015470042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325528.1 Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

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CloneID	OBa89744
Clone ID Related Accession (Same CDS sequence)	XM_015463073.1 , XM_015470042.1
Accession Version	XM_015470042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGG GACAACAAGC 
CCACGTTACT CTCCGTTCAG CCCATCCGTG CCACAGATGG GCAGCCCCAG TGCTCTGTAT 
AAAGGAGCTC ATGAACCCCG AGGCTGCCCT GAGATTCTCA GTCATTCTGT GGGGATGTCA 
GAGAGCCCTG TTGGACCCAA ACCCACGATG CTCCGGGCCG ACATGCCCAC GGCGCCCTCC 
TTCCAGCGGG CATTCACGTC CTCCTGCACC ATTTCTGGGA ACAGCCCCAG CCAGAGGAGA 
GGGAGCCCTT CCTCAGCTGA GCACCAGTGG GTGGAGACCA GCCCCAAGTC CACGCTGACC 
CTCCTGGGGG GCAGCCGGCC TGGCAAGGAC AGCCCCGCGC GACCCCACTT CCCGCCTGCC 
GACCTCCAGA CATCCTTCCA CGGCCCCGAG CTGTCCCTGG CGGAGCCGCC GGAAGCTCTG 
GGTCCCCCGA GCAACCACGC CTTCCTGAGC TTCAGCACTG CCCCCATGGC GGGAGGTGGG 
CTCCCCGCCG GGGAGGACCC CGGGGCCTTG CTGGCCAATT CCCACGGAGC AGCCCAGGCC 
CCTGGCAACC CACTGACGGC AGCTGAGGCT GCCGACAACA GCTTCCTGTC CCACAGCTTT 
CTCACGGTGG CACCTGGACA CAGCGGCCAC CACAGCCCGG TCCTGCAGGG CCCGGGACTG 
GCTCTGCCCG GGCAGCCACC CCTTCCTGAG AAGAAGCGGA CCTCAGAGGG AGATCGGTCT 
TTTGGCTCCG TCTCGCCATC CTCCAGTGGC TTCTCCAGTC CTCACAGCGG GAGTACCATG 
AGCATCCCTT TTCCAAACAT CATCCCGGAC TTTTCCAAGG CTGCCGAGGG GGCAGCACCT 
TCCCCAGATA ATCCAGGTGA TAAGCACGTG TCTGTAAACT TTGTTCAGGA CACATCCAAG 
TTCTGGTACA AGGCGGACAT CTCCAGAGAG CAAGCCATCG CCATGCTGAA GGACAAGGAG 
CCCGGGTCAT TCATCGTCAG GGACAGCCAC TCCTTCCGAG GTGCCTACGG CCTGGCCATG 
AAGGTGGCCA CACCCCCGCC CTCTGTCCTG CAGATGAACA AGAAAGCTGG AGATTTGGCC 
AATGAACTTG TTCGGCATTT TTTGATTGAG TGTACCCCAA AAGGAGTGCG GTTGAAAGGG 
TGCTCCAATG AACCATATTT CGGGAGCCTG ACGGCTTTGG TGTGTCAGCA TTCCATTACG 
CCCCTGGCTT TGCCCTGCAA ACTGCTCATT CCGGATAGAG ATCCATTGGA GGAAATAGCC 
GAAAATCCTC AGCAAACAGC GGCCAACTCT GCGGCCGAGC TGCTGAAGCA AGGGGCAGCC 
TGCAATGTGT GGTACCTGAA CTCCGTGGAG ATGGAGTCCC TCACGGGCCA CCAAGCCATC 
CAGAAGGCCC TGAGCATGAC CCTAGTCCAG GAGCCACCTC CCTTGTCCAC AGTCGTGCAC 
TTCAAGGTGT CGGCCCAGGG CATCACCCTG ACGGACAACC AGAGGAAGCT CTTCTTCCGG 
AGACACTACC CCGTGAGCAG CGTGATCTTC TGTGCGCTGG ACCCACAGGA CAGAAAGTGG 
GTCACAGACG GCCCTTGCTC GAGAGTCTTT GGATTTGTGG CCCGGAAGCA GGGCAGTGCC 
ACGGACAACG TGTGCCACCT GTTTGCAGAG CATGACCCCG AGCAGCCGGC CAGCGCCATT 
GTCAACTTCG TGTCAAAGGT CATGATCGGC TCCCCTAAGA AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325528.1
CDS	547..5334
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015470042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470042.1

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGG GACAACAAGC 
CCACGTTACT CTCCGTTCAG CCCATCCGTG CCACAGATGG GCAGCCCCAG TGCTCTGTAT 
AAAGGAGCTC ATGAACCCCG AGGCTGCCCT GAGATTCTCA GTCATTCTGT GGGGATGTCA 
GAGAGCCCTG TTGGACCCAA ACCCACGATG CTCCGGGCCG ACATGCCCAC GGCGCCCTCC 
TTCCAGCGGG CATTCACGTC CTCCTGCACC ATTTCTGGGA ACAGCCCCAG CCAGAGGAGA 
GGGAGCCCTT CCTCAGCTGA GCACCAGTGG GTGGAGACCA GCCCCAAGTC CACGCTGACC 
CTCCTGGGGG GCAGCCGGCC TGGCAAGGAC AGCCCCGCGC GACCCCACTT CCCGCCTGCC 
GACCTCCAGA CATCCTTCCA CGGCCCCGAG CTGTCCCTGG CGGAGCCGCC GGAAGCTCTG 
GGTCCCCCGA GCAACCACGC CTTCCTGAGC TTCAGCACTG CCCCCATGGC GGGAGGTGGG 
CTCCCCGCCG GGGAGGACCC CGGGGCCTTG CTGGCCAATT CCCACGGAGC AGCCCAGGCC 
CCTGGCAACC CACTGACGGC AGCTGAGGCT GCCGACAACA GCTTCCTGTC CCACAGCTTT 
CTCACGGTGG CACCTGGACA CAGCGGCCAC CACAGCCCGG TCCTGCAGGG CCCGGGACTG 
GCTCTGCCCG GGCAGCCACC CCTTCCTGAG AAGAAGCGGA CCTCAGAGGG AGATCGGTCT 
TTTGGCTCCG TCTCGCCATC CTCCAGTGGC TTCTCCAGTC CTCACAGCGG GAGTACCATG 
AGCATCCCTT TTCCAAACAT CATCCCGGAC TTTTCCAAGG CTGCCGAGGG GGCAGCACCT 
TCCCCAGATA ATCCAGGTGA TAAGCACGTG TCTGTAAACT TTGTTCAGGA CACATCCAAG 
TTCTGGTACA AGGCGGACAT CTCCAGAGAG CAAGCCATCG CCATGCTGAA GGACAAGGAG 
CCCGGGTCAT TCATCGTCAG GGACAGCCAC TCCTTCCGAG GTGCCTACGG CCTGGCCATG 
AAGGTGGCCA CACCCCCGCC CTCTGTCCTG CAGATGAACA AGAAAGCTGG AGATTTGGCC 
AATGAACTTG TTCGGCATTT TTTGATTGAG TGTACCCCAA AAGGAGTGCG GTTGAAAGGG 
TGCTCCAATG AACCATATTT CGGGAGCCTG ACGGCTTTGG TGTGTCAGCA TTCCATTACG 
CCCCTGGCTT TGCCCTGCAA ACTGCTCATT CCGGATAGAG ATCCATTGGA GGAAATAGCC 
GAAAATCCTC AGCAAACAGC GGCCAACTCT GCGGCCGAGC TGCTGAAGCA AGGGGCAGCC 
TGCAATGTGT GGTACCTGAA CTCCGTGGAG ATGGAGTCCC TCACGGGCCA CCAAGCCATC 
CAGAAGGCCC TGAGCATGAC CCTAGTCCAG GAGCCACCTC CCTTGTCCAC AGTCGTGCAC 
TTCAAGGTGT CGGCCCAGGG CATCACCCTG ACGGACAACC AGAGGAAGCT CTTCTTCCGG 
AGACACTACC CCGTGAGCAG CGTGATCTTC TGTGCGCTGG ACCCACAGGA CAGAAAGTGG 
GTCACAGACG GCCCTTGCTC GAGAGTCTTT GGATTTGTGG CCCGGAAGCA GGGCAGTGCC 
ACGGACAACG TGTGCCACCT GTTTGCAGAG CATGACCCCG AGCAGCCGGC CAGCGCCATT 
GTCAACTTCG TGTCAAAGGT CATGATCGGC TCCCCTAAGA AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010804393.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802695.2 Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89745
Clone ID Related Accession (Same CDS sequence)	XM_010804393.2 , XM_010827362.2
Accession Version	XM_010804393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GCTCCGGGGG CATCATCAAC ACCTGCTTCT CTGTGCTGTG ACAAACCGTT CAGACCTGCA 
GGGCTGAGCC CCGCCATGGA GGACGGCCGC GAGCTGGATC TCACCTACAT CACCGAGCGC 
ATCATTGCCG TGTCCTTCCC CGCGGGCTGC TCCGAGGAGT CTTACCTGCA CAACCTGCAG 
GAGGTGACGC GCATGCTACG CTCCAAGCAT GGGGACAACT ACTTGGTGTT AAACCTCTCG 
GAAAAGAGAT ATGACCTTAC AAAGCTTAAC CCAAAGATTC TGGACGTGGG CTGGCCGGAG 
CTGCATGCGC CACCCCTGGA TAAGGTGTGC ACCATCTGCA AGGCGCAGGA GGCCTGGCTG 
AACAGCGACC CCCAACACGT GGTTGTTATT CACTGCAGGG GTGGAAAGGG ACGCATCGGC 
GTGGTCATAT CATCTTACAT GCACTTCACC AATGTCTCGG CCAGTGCTGA CCAGGCCCTT 
GACAGATTCG CAATGAAGAA GTTTTATGAT GATAAACTTG CAGCTTTAAT GCAGCCATCC 
CAGAAACGGT ATGTGCAGTT TCTCAGTGGG CTCCTGTCAG GAACGGTGAA GATGAATGCC 
TCGCCCCTGT TCCTGCATTT CGTCATCCTG CACGGAACCC CCAATTTTGA CTCCGGTGGA 
GTGTGCCGGC CCTTTCTCAA GCTCTACCAG GCCATGCAGC CCGTGTACAC GTCGGGGATC 
TACAATGTTG GCCCAGAAAA CCAAAGCAGG ATCTACATTG CCATCGAGCC GGCCCAGCTC 
CTGAAAGGGG ACATCATGGT GAAGTGCTAC CACAAGAAGT ACCGCTCGGC CACCCGTGAT 
GTCATTTTCC GCCTGCAGTT TCACACCGGG GCTGTTCAGG GCTACAGACT TGTGTTTGCG 
AAGGAGGATT TGGACAACGC GAGCAAAGAT GACCGTTTTC CTGATAATGG GAAGATTGAG 
TTAGTCTTCT CAGCCACTCC TGAGAAGATT CAAGGATGTG AACATTTGCA GAATGACCAC 
AGAGTGATTG TGGACTTCAA CACAGCAGAC CCGCTAATCC GCTGGGACTC CTACGAGAAC 
CTGAGTGCAG ACGGTGAAGT GCTGCACACG CAGGGCCCCG TGGACGGCAG TCTTTACGCC 
AAGGTGAGAA AGAAGAGCAC TTCAGATCCC AGTGTCCCAG GCGGGTCCCC CGCTGTCCCA 
GCCGCCAGCA GCCCAGACCA CAGCGATCAC ACCCTGTCTG TCAGCAGTGA CTCGGGCCAC 
TCCACAGCCT CTGTGAGGAC CGACAGGACC GAGGAGCACC TGACTCCCGG ATCCAAGAGG 
GGCCTGAGCC CCCAGGAAAA GGCCGAGCTG GATCAGTTGC TCAGTGGCTT TGGCCTCGAG 
GATTCTGGAA GCCCCCTGAA GGATATGAGC GATGCTCGCA GCAAGTACAG CGGGACCCGC 
CACGTTGTAC CGGCCCAGGT CCACGTGAAC GGAGACACCA CCCCCAAGGA CCGAGAGACG 
GACATCCTGG ATGACGAGAT GCCCAGCCAC GACCTGCACA GCGTGGACAG CATCGGGACC 
CTGTCCTCCT CGGAGGGCCA CCCGTCAGCC CACCTGAGCC CCTTCACCTG CCACCAGAGC 
AGCCAGAACT CCCTCCTGTC TGATGGATTC GGCAGCACTG CCGGCGAAGA TCAGCATGGT 
GCTCTGGCCC CAGATCTGGG GCTCGCCGCG GAGCCCCTGT TCGAGCGAGA GCGGGCTTTC 
GGGAGCTGCG AGCCCAAGCA GCCACAGCCT GTGCTCAGGA AGCCCTCTGT GCCTGCCCAG 
ACGCAGGCCT ACGGGCCGAG CAGCTACTCC ACGCAGACCT GGGTGCGCCA GCAGCAGATG 
GTGGCCGCTC ACCAGTACAG CTTTGCCCCT GATGGGGAGG CCAGGCTCAG CGGCCGTGGT 
GCAGCAGACA GTTCTGGCCT GCTGCAGACA CAGCCTAGGG TCCCGCTCAC CCCCACTCGG 
GGGGCCAGCA GCAGAGTGGC CGTCCAGAGG GGCATAGGCC CTGGGCCACA TCCTCCCGAT 
ATACAGAGAC CCCCTCCTGG CAAAGCAGCC TTCAAACCCA GGATTCCAGA TGTCAAAGTC 
GTGAATGGTG CTGGCCCAGA GCTGGGCACA GACCTGTCCC CAGGCTCACC CACCCTGGAC 
ATCGACCAGT CGATCGAACA GCTCAACAGG CTGATCTTGG AGCTGGACCC CACCTTTGAG 
CCCATCCCCA CCCACATGAA CATGTCGAGC AGCCAGGCCA ACGGCCCCAT GCCCCCAGAC 
GGCATGGCAG GCGGGCTATG GGCCAGTGGC CGCCTGCAGG ACACAGGAGA TGGCCCTGGC 
AGGGCCCCAG TGAGGCAAGG GGATGAGCCA CTTGGGGGAA GATTCCGGAA GCTAAGCCTT 
GAGCAGTATG ATAACGAAGC TGGGGAGCAG CCCACCTTCT CCAAATGCAG CTGGGGAAAG 
ACCACCAGTT CAGAACAGAC TCCAGGCCTG GCGCCCTTTC TCTCCCCAGA CAACACCAAA 
GAGGCAGTGA TCACCACGTA TCCCCCAGAC CTGGATGTGA TCGATGGCAG GATCTTCTCT 
CCAACCAACA GTGGCAGTGA ATCCATCTCT CCCACCCCTG CATTTCCTGT GTCTCCAGAA 
ACGCCGTATG GGACAACAAG CCCACGTTAC TCTCCGTTCA GCCCATCCGT GCCACAGATG 
GGCAGCCCCA GTGCTCTGTA TAAAGGAGCT CATGAACCCC GAGGCTGCCC TGAGATTCTC 
AGTCATTCTG TGGGGATGTC AGAGAGCCCT GTTGGACCCA AACCCACGAT GCTCCGGGCC 
GACATGCCCA CGGCGCCCTC CTTCCAGCGG GCATTCACGT CCTCCTGCAC CATTTCTGGG 
AACAGCCCCA GCCAGAGGAG AGGGAGCCCT TCCTCAGCTG AGCACCAGTG GGTGGAGACC 
AGCCCCAAGT CCACGCTGAC CCTCCTGGGG GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG 
CGACCCCACT TCCCGCCTGC CGACCTCCAG ACATCCTTCC ACGGCCCCGA GCTGTCCCTG 
GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG AGCAACCACG CCTTCCTGAG CTTCAGCACT 
GCCCCCATGG CGGGAGGTGG GCTCCCCGCC GGGGAGGACC CCGGGGCCTT GCTGGCCAAT 
TCCCACGGAG CAGCCCAGGC CCCTGGCAAC CCACTGACGG CAGCTGAGGC TGCCGACAAC 
AGCTTCCTGT CCCACAGCTT TCTCACGGTG GCACCTGGAC ACAGCGGCCA CCACAGCCCG 
GTCCTGCAGG GCCCGGGACT GGCTCTGCCC GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG 
ACCTCAGAGG GAGATCGGTC TTTTGGCTCC GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT 
CCTCACAGCG GGAGTACCAT GAGCATCCCT TTTCCAAACA TCATCCCGGA CTTTTCCAAG 
GCTGCCGAGG GGGCAGCACC TTCCCCAGAT AATCCAGGTG ATAAGCACGT GTCTGTAAAC 
TTTGTTCAGG ACACATCCAA GTTCTGGTAC AAGGCGGACA TCTCCAGAGA GCAAGCCATC 
GCCATGCTGA AGGACAAGGA GCCCGGGTCA TTCATCGTCA GGGACAGCCA CTCCTTCCGA 
GGTGCCTACG GCCTGGCCAT GAAGGTGGCC ACACCCCCGC CCTCTGTCCT GCAGATGAAC 
AAGAAAGCTG GAGATTTGGC CAATGAACTT GTTCGGCATT TTTTGATTGA GTGTACCCCA 
AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT GAACCATATT TCGGGAGCCT GACGGCTTTG 
GTGTGTCAGC ATTCCATTAC GCCCCTGGCT TTGCCCTGCA AACTGCTCAT TCCGGATAGA 
GATCCATTGG AGGAAATAGC CGAAAATCCT CAGCAAACAG CGGCCAACTC TGCGGCCGAG 
CTGCTGAAGC AAGGGGCAGC CTGCAATGTG TGGTACCTGA ACTCCGTGGA GATGGAGTCC 
CTCACGGGCC ACCAAGCCAT CCAGAAGGCC CTGAGCATGA CCCTAGTCCA GGAGCCACCT 
CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG TCGGCCCAGG GCATCACCCT GACGGACAAC 
CAGAGGAAGC TCTTCTTCCG GAGACACTAC CCCGTGAGCA GCGTGATCTT CTGTGCGCTG 
GACCCACAGG ACAGAAAGTG GGTCACAGAC GGCCCTTGCT CGAGAGTCTT TGGATTTGTG 
GCCCGGAAGC AGGGCAGTGC CACGGACAAC GTGTGCCACC TGTTTGCAGA GCATGACCCC 
GAGCAGCCGG CCAGCGCCAT TGTCAACTTC GTGTCAAAGG TCATGATCGG CTCCCCTAAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802695.2
CDS	547..5295
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010804393.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804393.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GCTCCGGGGG CATCATCAAC ACCTGCTTCT CTGTGCTGTG ACAAACCGTT CAGACCTGCA 
GGGCTGAGCC CCGCCATGGA GGACGGCCGC GAGCTGGATC TCACCTACAT CACCGAGCGC 
ATCATTGCCG TGTCCTTCCC CGCGGGCTGC TCCGAGGAGT CTTACCTGCA CAACCTGCAG 
GAGGTGACGC GCATGCTACG CTCCAAGCAT GGGGACAACT ACTTGGTGTT AAACCTCTCG 
GAAAAGAGAT ATGACCTTAC AAAGCTTAAC CCAAAGATTC TGGACGTGGG CTGGCCGGAG 
CTGCATGCGC CACCCCTGGA TAAGGTGTGC ACCATCTGCA AGGCGCAGGA GGCCTGGCTG 
AACAGCGACC CCCAACACGT GGTTGTTATT CACTGCAGGG GTGGAAAGGG ACGCATCGGC 
GTGGTCATAT CATCTTACAT GCACTTCACC AATGTCTCGG CCAGTGCTGA CCAGGCCCTT 
GACAGATTCG CAATGAAGAA GTTTTATGAT GATAAACTTG CAGCTTTAAT GCAGCCATCC 
CAGAAACGGT ATGTGCAGTT TCTCAGTGGG CTCCTGTCAG GAACGGTGAA GATGAATGCC 
TCGCCCCTGT TCCTGCATTT CGTCATCCTG CACGGAACCC CCAATTTTGA CTCCGGTGGA 
GTGTGCCGGC CCTTTCTCAA GCTCTACCAG GCCATGCAGC CCGTGTACAC GTCGGGGATC 
TACAATGTTG GCCCAGAAAA CCAAAGCAGG ATCTACATTG CCATCGAGCC GGCCCAGCTC 
CTGAAAGGGG ACATCATGGT GAAGTGCTAC CACAAGAAGT ACCGCTCGGC CACCCGTGAT 
GTCATTTTCC GCCTGCAGTT TCACACCGGG GCTGTTCAGG GCTACAGACT TGTGTTTGCG 
AAGGAGGATT TGGACAACGC GAGCAAAGAT GACCGTTTTC CTGATAATGG GAAGATTGAG 
TTAGTCTTCT CAGCCACTCC TGAGAAGATT CAAGGATGTG AACATTTGCA GAATGACCAC 
AGAGTGATTG TGGACTTCAA CACAGCAGAC CCGCTAATCC GCTGGGACTC CTACGAGAAC 
CTGAGTGCAG ACGGTGAAGT GCTGCACACG CAGGGCCCCG TGGACGGCAG TCTTTACGCC 
AAGGTGAGAA AGAAGAGCAC TTCAGATCCC AGTGTCCCAG GCGGGTCCCC CGCTGTCCCA 
GCCGCCAGCA GCCCAGACCA CAGCGATCAC ACCCTGTCTG TCAGCAGTGA CTCGGGCCAC 
TCCACAGCCT CTGTGAGGAC CGACAGGACC GAGGAGCACC TGACTCCCGG ATCCAAGAGG 
GGCCTGAGCC CCCAGGAAAA GGCCGAGCTG GATCAGTTGC TCAGTGGCTT TGGCCTCGAG 
GATTCTGGAA GCCCCCTGAA GGATATGAGC GATGCTCGCA GCAAGTACAG CGGGACCCGC 
CACGTTGTAC CGGCCCAGGT CCACGTGAAC GGAGACACCA CCCCCAAGGA CCGAGAGACG 
GACATCCTGG ATGACGAGAT GCCCAGCCAC GACCTGCACA GCGTGGACAG CATCGGGACC 
CTGTCCTCCT CGGAGGGCCA CCCGTCAGCC CACCTGAGCC CCTTCACCTG CCACCAGAGC 
AGCCAGAACT CCCTCCTGTC TGATGGATTC GGCAGCACTG CCGGCGAAGA TCAGCATGGT 
GCTCTGGCCC CAGATCTGGG GCTCGCCGCG GAGCCCCTGT TCGAGCGAGA GCGGGCTTTC 
GGGAGCTGCG AGCCCAAGCA GCCACAGCCT GTGCTCAGGA AGCCCTCTGT GCCTGCCCAG 
ACGCAGGCCT ACGGGCCGAG CAGCTACTCC ACGCAGACCT GGGTGCGCCA GCAGCAGATG 
GTGGCCGCTC ACCAGTACAG CTTTGCCCCT GATGGGGAGG CCAGGCTCAG CGGCCGTGGT 
GCAGCAGACA GTTCTGGCCT GCTGCAGACA CAGCCTAGGG TCCCGCTCAC CCCCACTCGG 
GGGGCCAGCA GCAGAGTGGC CGTCCAGAGG GGCATAGGCC CTGGGCCACA TCCTCCCGAT 
ATACAGAGAC CCCCTCCTGG CAAAGCAGCC TTCAAACCCA GGATTCCAGA TGTCAAAGTC 
GTGAATGGTG CTGGCCCAGA GCTGGGCACA GACCTGTCCC CAGGCTCACC CACCCTGGAC 
ATCGACCAGT CGATCGAACA GCTCAACAGG CTGATCTTGG AGCTGGACCC CACCTTTGAG 
CCCATCCCCA CCCACATGAA CATGTCGAGC AGCCAGGCCA ACGGCCCCAT GCCCCCAGAC 
GGCATGGCAG GCGGGCTATG GGCCAGTGGC CGCCTGCAGG ACACAGGAGA TGGCCCTGGC 
AGGGCCCCAG TGAGGCAAGG GGATGAGCCA CTTGGGGGAA GATTCCGGAA GCTAAGCCTT 
GAGCAGTATG ATAACGAAGC TGGGGAGCAG CCCACCTTCT CCAAATGCAG CTGGGGAAAG 
ACCACCAGTT CAGAACAGAC TCCAGGCCTG GCGCCCTTTC TCTCCCCAGA CAACACCAAA 
GAGGCAGTGA TCACCACGTA TCCCCCAGAC CTGGATGTGA TCGATGGCAG GATCTTCTCT 
CCAACCAACA GTGGCAGTGA ATCCATCTCT CCCACCCCTG CATTTCCTGT GTCTCCAGAA 
ACGCCGTATG GGACAACAAG CCCACGTTAC TCTCCGTTCA GCCCATCCGT GCCACAGATG 
GGCAGCCCCA GTGCTCTGTA TAAAGGAGCT CATGAACCCC GAGGCTGCCC TGAGATTCTC 
AGTCATTCTG TGGGGATGTC AGAGAGCCCT GTTGGACCCA AACCCACGAT GCTCCGGGCC 
GACATGCCCA CGGCGCCCTC CTTCCAGCGG GCATTCACGT CCTCCTGCAC CATTTCTGGG 
AACAGCCCCA GCCAGAGGAG AGGGAGCCCT TCCTCAGCTG AGCACCAGTG GGTGGAGACC 
AGCCCCAAGT CCACGCTGAC CCTCCTGGGG GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG 
CGACCCCACT TCCCGCCTGC CGACCTCCAG ACATCCTTCC ACGGCCCCGA GCTGTCCCTG 
GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG AGCAACCACG CCTTCCTGAG CTTCAGCACT 
GCCCCCATGG CGGGAGGTGG GCTCCCCGCC GGGGAGGACC CCGGGGCCTT GCTGGCCAAT 
TCCCACGGAG CAGCCCAGGC CCCTGGCAAC CCACTGACGG CAGCTGAGGC TGCCGACAAC 
AGCTTCCTGT CCCACAGCTT TCTCACGGTG GCACCTGGAC ACAGCGGCCA CCACAGCCCG 
GTCCTGCAGG GCCCGGGACT GGCTCTGCCC GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG 
ACCTCAGAGG GAGATCGGTC TTTTGGCTCC GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT 
CCTCACAGCG GGAGTACCAT GAGCATCCCT TTTCCAAACA TCATCCCGGA CTTTTCCAAG 
GCTGCCGAGG GGGCAGCACC TTCCCCAGAT AATCCAGGTG ATAAGCACGT GTCTGTAAAC 
TTTGTTCAGG ACACATCCAA GTTCTGGTAC AAGGCGGACA TCTCCAGAGA GCAAGCCATC 
GCCATGCTGA AGGACAAGGA GCCCGGGTCA TTCATCGTCA GGGACAGCCA CTCCTTCCGA 
GGTGCCTACG GCCTGGCCAT GAAGGTGGCC ACACCCCCGC CCTCTGTCCT GCAGATGAAC 
AAGAAAGCTG GAGATTTGGC CAATGAACTT GTTCGGCATT TTTTGATTGA GTGTACCCCA 
AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT GAACCATATT TCGGGAGCCT GACGGCTTTG 
GTGTGTCAGC ATTCCATTAC GCCCCTGGCT TTGCCCTGCA AACTGCTCAT TCCGGATAGA 
GATCCATTGG AGGAAATAGC CGAAAATCCT CAGCAAACAG CGGCCAACTC TGCGGCCGAG 
CTGCTGAAGC AAGGGGCAGC CTGCAATGTG TGGTACCTGA ACTCCGTGGA GATGGAGTCC 
CTCACGGGCC ACCAAGCCAT CCAGAAGGCC CTGAGCATGA CCCTAGTCCA GGAGCCACCT 
CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG TCGGCCCAGG GCATCACCCT GACGGACAAC 
CAGAGGAAGC TCTTCTTCCG GAGACACTAC CCCGTGAGCA GCGTGATCTT CTGTGCGCTG 
GACCCACAGG ACAGAAAGTG GGTCACAGAC GGCCCTTGCT CGAGAGTCTT TGGATTTGTG 
GCCCGGAAGC AGGGCAGTGC CACGGACAAC GTGTGCCACC TGTTTGCAGA GCATGACCCC 
GAGCAGCCGG CCAGCGCCAT TGTCAACTTC GTGTCAAAGG TCATGATCGG CTCCCCTAAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010827362.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825664.2 Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89745
Clone ID Related Accession (Same CDS sequence)	XM_010804393.2 , XM_010827362.2
Accession Version	XM_010827362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GCTCCGGGGG CATCATCAAC ACCTGCTTCT CTGTGCTGTG ACAAACCGTT CAGACCTGCA 
GGGCTGAGCC CCGCCATGGA GGACGGCCGC GAGCTGGATC TCACCTACAT CACCGAGCGC 
ATCATTGCCG TGTCCTTCCC CGCGGGCTGC TCCGAGGAGT CTTACCTGCA CAACCTGCAG 
GAGGTGACGC GCATGCTACG CTCCAAGCAT GGGGACAACT ACTTGGTGTT AAACCTCTCG 
GAAAAGAGAT ATGACCTTAC AAAGCTTAAC CCAAAGATTC TGGACGTGGG CTGGCCGGAG 
CTGCATGCGC CACCCCTGGA TAAGGTGTGC ACCATCTGCA AGGCGCAGGA GGCCTGGCTG 
AACAGCGACC CCCAACACGT GGTTGTTATT CACTGCAGGG GTGGAAAGGG ACGCATCGGC 
GTGGTCATAT CATCTTACAT GCACTTCACC AATGTCTCGG CCAGTGCTGA CCAGGCCCTT 
GACAGATTCG CAATGAAGAA GTTTTATGAT GATAAACTTG CAGCTTTAAT GCAGCCATCC 
CAGAAACGGT ATGTGCAGTT TCTCAGTGGG CTCCTGTCAG GAACGGTGAA GATGAATGCC 
TCGCCCCTGT TCCTGCATTT CGTCATCCTG CACGGAACCC CCAATTTTGA CTCCGGTGGA 
GTGTGCCGGC CCTTTCTCAA GCTCTACCAG GCCATGCAGC CCGTGTACAC GTCGGGGATC 
TACAATGTTG GCCCAGAAAA CCAAAGCAGG ATCTACATTG CCATCGAGCC GGCCCAGCTC 
CTGAAAGGGG ACATCATGGT GAAGTGCTAC CACAAGAAGT ACCGCTCGGC CACCCGTGAT 
GTCATTTTCC GCCTGCAGTT TCACACCGGG GCTGTTCAGG GCTACAGACT TGTGTTTGCG 
AAGGAGGATT TGGACAACGC GAGCAAAGAT GACCGTTTTC CTGATAATGG GAAGATTGAG 
TTAGTCTTCT CAGCCACTCC TGAGAAGATT CAAGGATGTG AACATTTGCA GAATGACCAC 
AGAGTGATTG TGGACTTCAA CACAGCAGAC CCGCTAATCC GCTGGGACTC CTACGAGAAC 
CTGAGTGCAG ACGGTGAAGT GCTGCACACG CAGGGCCCCG TGGACGGCAG TCTTTACGCC 
AAGGTGAGAA AGAAGAGCAC TTCAGATCCC AGTGTCCCAG GCGGGTCCCC CGCTGTCCCA 
GCCGCCAGCA GCCCAGACCA CAGCGATCAC ACCCTGTCTG TCAGCAGTGA CTCGGGCCAC 
TCCACAGCCT CTGTGAGGAC CGACAGGACC GAGGAGCACC TGACTCCCGG ATCCAAGAGG 
GGCCTGAGCC CCCAGGAAAA GGCCGAGCTG GATCAGTTGC TCAGTGGCTT TGGCCTCGAG 
GATTCTGGAA GCCCCCTGAA GGATATGAGC GATGCTCGCA GCAAGTACAG CGGGACCCGC 
CACGTTGTAC CGGCCCAGGT CCACGTGAAC GGAGACACCA CCCCCAAGGA CCGAGAGACG 
GACATCCTGG ATGACGAGAT GCCCAGCCAC GACCTGCACA GCGTGGACAG CATCGGGACC 
CTGTCCTCCT CGGAGGGCCA CCCGTCAGCC CACCTGAGCC CCTTCACCTG CCACCAGAGC 
AGCCAGAACT CCCTCCTGTC TGATGGATTC GGCAGCACTG CCGGCGAAGA TCAGCATGGT 
GCTCTGGCCC CAGATCTGGG GCTCGCCGCG GAGCCCCTGT TCGAGCGAGA GCGGGCTTTC 
GGGAGCTGCG AGCCCAAGCA GCCACAGCCT GTGCTCAGGA AGCCCTCTGT GCCTGCCCAG 
ACGCAGGCCT ACGGGCCGAG CAGCTACTCC ACGCAGACCT GGGTGCGCCA GCAGCAGATG 
GTGGCCGCTC ACCAGTACAG CTTTGCCCCT GATGGGGAGG CCAGGCTCAG CGGCCGTGGT 
GCAGCAGACA GTTCTGGCCT GCTGCAGACA CAGCCTAGGG TCCCGCTCAC CCCCACTCGG 
GGGGCCAGCA GCAGAGTGGC CGTCCAGAGG GGCATAGGCC CTGGGCCACA TCCTCCCGAT 
ATACAGAGAC CCCCTCCTGG CAAAGCAGCC TTCAAACCCA GGATTCCAGA TGTCAAAGTC 
GTGAATGGTG CTGGCCCAGA GCTGGGCACA GACCTGTCCC CAGGCTCACC CACCCTGGAC 
ATCGACCAGT CGATCGAACA GCTCAACAGG CTGATCTTGG AGCTGGACCC CACCTTTGAG 
CCCATCCCCA CCCACATGAA CATGTCGAGC AGCCAGGCCA ACGGCCCCAT GCCCCCAGAC 
GGCATGGCAG GCGGGCTATG GGCCAGTGGC CGCCTGCAGG ACACAGGAGA TGGCCCTGGC 
AGGGCCCCAG TGAGGCAAGG GGATGAGCCA CTTGGGGGAA GATTCCGGAA GCTAAGCCTT 
GAGCAGTATG ATAACGAAGC TGGGGAGCAG CCCACCTTCT CCAAATGCAG CTGGGGAAAG 
ACCACCAGTT CAGAACAGAC TCCAGGCCTG GCGCCCTTTC TCTCCCCAGA CAACACCAAA 
GAGGCAGTGA TCACCACGTA TCCCCCAGAC CTGGATGTGA TCGATGGCAG GATCTTCTCT 
CCAACCAACA GTGGCAGTGA ATCCATCTCT CCCACCCCTG CATTTCCTGT GTCTCCAGAA 
ACGCCGTATG GGACAACAAG CCCACGTTAC TCTCCGTTCA GCCCATCCGT GCCACAGATG 
GGCAGCCCCA GTGCTCTGTA TAAAGGAGCT CATGAACCCC GAGGCTGCCC TGAGATTCTC 
AGTCATTCTG TGGGGATGTC AGAGAGCCCT GTTGGACCCA AACCCACGAT GCTCCGGGCC 
GACATGCCCA CGGCGCCCTC CTTCCAGCGG GCATTCACGT CCTCCTGCAC CATTTCTGGG 
AACAGCCCCA GCCAGAGGAG AGGGAGCCCT TCCTCAGCTG AGCACCAGTG GGTGGAGACC 
AGCCCCAAGT CCACGCTGAC CCTCCTGGGG GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG 
CGACCCCACT TCCCGCCTGC CGACCTCCAG ACATCCTTCC ACGGCCCCGA GCTGTCCCTG 
GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG AGCAACCACG CCTTCCTGAG CTTCAGCACT 
GCCCCCATGG CGGGAGGTGG GCTCCCCGCC GGGGAGGACC CCGGGGCCTT GCTGGCCAAT 
TCCCACGGAG CAGCCCAGGC CCCTGGCAAC CCACTGACGG CAGCTGAGGC TGCCGACAAC 
AGCTTCCTGT CCCACAGCTT TCTCACGGTG GCACCTGGAC ACAGCGGCCA CCACAGCCCG 
GTCCTGCAGG GCCCGGGACT GGCTCTGCCC GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG 
ACCTCAGAGG GAGATCGGTC TTTTGGCTCC GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT 
CCTCACAGCG GGAGTACCAT GAGCATCCCT TTTCCAAACA TCATCCCGGA CTTTTCCAAG 
GCTGCCGAGG GGGCAGCACC TTCCCCAGAT AATCCAGGTG ATAAGCACGT GTCTGTAAAC 
TTTGTTCAGG ACACATCCAA GTTCTGGTAC AAGGCGGACA TCTCCAGAGA GCAAGCCATC 
GCCATGCTGA AGGACAAGGA GCCCGGGTCA TTCATCGTCA GGGACAGCCA CTCCTTCCGA 
GGTGCCTACG GCCTGGCCAT GAAGGTGGCC ACACCCCCGC CCTCTGTCCT GCAGATGAAC 
AAGAAAGCTG GAGATTTGGC CAATGAACTT GTTCGGCATT TTTTGATTGA GTGTACCCCA 
AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT GAACCATATT TCGGGAGCCT GACGGCTTTG 
GTGTGTCAGC ATTCCATTAC GCCCCTGGCT TTGCCCTGCA AACTGCTCAT TCCGGATAGA 
GATCCATTGG AGGAAATAGC CGAAAATCCT CAGCAAACAG CGGCCAACTC TGCGGCCGAG 
CTGCTGAAGC AAGGGGCAGC CTGCAATGTG TGGTACCTGA ACTCCGTGGA GATGGAGTCC 
CTCACGGGCC ACCAAGCCAT CCAGAAGGCC CTGAGCATGA CCCTAGTCCA GGAGCCACCT 
CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG TCGGCCCAGG GCATCACCCT GACGGACAAC 
CAGAGGAAGC TCTTCTTCCG GAGACACTAC CCCGTGAGCA GCGTGATCTT CTGTGCGCTG 
GACCCACAGG ACAGAAAGTG GGTCACAGAC GGCCCTTGCT CGAGAGTCTT TGGATTTGTG 
GCCCGGAAGC AGGGCAGTGC CACGGACAAC GTGTGCCACC TGTTTGCAGA GCATGACCCC 
GAGCAGCCGG CCAGCGCCAT TGTCAACTTC GTGTCAAAGG TCATGATCGG CTCCCCTAAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825664.2
CDS	547..5295
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010827362.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827362.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GCTCCGGGGG CATCATCAAC ACCTGCTTCT CTGTGCTGTG ACAAACCGTT CAGACCTGCA 
GGGCTGAGCC CCGCCATGGA GGACGGCCGC GAGCTGGATC TCACCTACAT CACCGAGCGC 
ATCATTGCCG TGTCCTTCCC CGCGGGCTGC TCCGAGGAGT CTTACCTGCA CAACCTGCAG 
GAGGTGACGC GCATGCTACG CTCCAAGCAT GGGGACAACT ACTTGGTGTT AAACCTCTCG 
GAAAAGAGAT ATGACCTTAC AAAGCTTAAC CCAAAGATTC TGGACGTGGG CTGGCCGGAG 
CTGCATGCGC CACCCCTGGA TAAGGTGTGC ACCATCTGCA AGGCGCAGGA GGCCTGGCTG 
AACAGCGACC CCCAACACGT GGTTGTTATT CACTGCAGGG GTGGAAAGGG ACGCATCGGC 
GTGGTCATAT CATCTTACAT GCACTTCACC AATGTCTCGG CCAGTGCTGA CCAGGCCCTT 
GACAGATTCG CAATGAAGAA GTTTTATGAT GATAAACTTG CAGCTTTAAT GCAGCCATCC 
CAGAAACGGT ATGTGCAGTT TCTCAGTGGG CTCCTGTCAG GAACGGTGAA GATGAATGCC 
TCGCCCCTGT TCCTGCATTT CGTCATCCTG CACGGAACCC CCAATTTTGA CTCCGGTGGA 
GTGTGCCGGC CCTTTCTCAA GCTCTACCAG GCCATGCAGC CCGTGTACAC GTCGGGGATC 
TACAATGTTG GCCCAGAAAA CCAAAGCAGG ATCTACATTG CCATCGAGCC GGCCCAGCTC 
CTGAAAGGGG ACATCATGGT GAAGTGCTAC CACAAGAAGT ACCGCTCGGC CACCCGTGAT 
GTCATTTTCC GCCTGCAGTT TCACACCGGG GCTGTTCAGG GCTACAGACT TGTGTTTGCG 
AAGGAGGATT TGGACAACGC GAGCAAAGAT GACCGTTTTC CTGATAATGG GAAGATTGAG 
TTAGTCTTCT CAGCCACTCC TGAGAAGATT CAAGGATGTG AACATTTGCA GAATGACCAC 
AGAGTGATTG TGGACTTCAA CACAGCAGAC CCGCTAATCC GCTGGGACTC CTACGAGAAC 
CTGAGTGCAG ACGGTGAAGT GCTGCACACG CAGGGCCCCG TGGACGGCAG TCTTTACGCC 
AAGGTGAGAA AGAAGAGCAC TTCAGATCCC AGTGTCCCAG GCGGGTCCCC CGCTGTCCCA 
GCCGCCAGCA GCCCAGACCA CAGCGATCAC ACCCTGTCTG TCAGCAGTGA CTCGGGCCAC 
TCCACAGCCT CTGTGAGGAC CGACAGGACC GAGGAGCACC TGACTCCCGG ATCCAAGAGG 
GGCCTGAGCC CCCAGGAAAA GGCCGAGCTG GATCAGTTGC TCAGTGGCTT TGGCCTCGAG 
GATTCTGGAA GCCCCCTGAA GGATATGAGC GATGCTCGCA GCAAGTACAG CGGGACCCGC 
CACGTTGTAC CGGCCCAGGT CCACGTGAAC GGAGACACCA CCCCCAAGGA CCGAGAGACG 
GACATCCTGG ATGACGAGAT GCCCAGCCAC GACCTGCACA GCGTGGACAG CATCGGGACC 
CTGTCCTCCT CGGAGGGCCA CCCGTCAGCC CACCTGAGCC CCTTCACCTG CCACCAGAGC 
AGCCAGAACT CCCTCCTGTC TGATGGATTC GGCAGCACTG CCGGCGAAGA TCAGCATGGT 
GCTCTGGCCC CAGATCTGGG GCTCGCCGCG GAGCCCCTGT TCGAGCGAGA GCGGGCTTTC 
GGGAGCTGCG AGCCCAAGCA GCCACAGCCT GTGCTCAGGA AGCCCTCTGT GCCTGCCCAG 
ACGCAGGCCT ACGGGCCGAG CAGCTACTCC ACGCAGACCT GGGTGCGCCA GCAGCAGATG 
GTGGCCGCTC ACCAGTACAG CTTTGCCCCT GATGGGGAGG CCAGGCTCAG CGGCCGTGGT 
GCAGCAGACA GTTCTGGCCT GCTGCAGACA CAGCCTAGGG TCCCGCTCAC CCCCACTCGG 
GGGGCCAGCA GCAGAGTGGC CGTCCAGAGG GGCATAGGCC CTGGGCCACA TCCTCCCGAT 
ATACAGAGAC CCCCTCCTGG CAAAGCAGCC TTCAAACCCA GGATTCCAGA TGTCAAAGTC 
GTGAATGGTG CTGGCCCAGA GCTGGGCACA GACCTGTCCC CAGGCTCACC CACCCTGGAC 
ATCGACCAGT CGATCGAACA GCTCAACAGG CTGATCTTGG AGCTGGACCC CACCTTTGAG 
CCCATCCCCA CCCACATGAA CATGTCGAGC AGCCAGGCCA ACGGCCCCAT GCCCCCAGAC 
GGCATGGCAG GCGGGCTATG GGCCAGTGGC CGCCTGCAGG ACACAGGAGA TGGCCCTGGC 
AGGGCCCCAG TGAGGCAAGG GGATGAGCCA CTTGGGGGAA GATTCCGGAA GCTAAGCCTT 
GAGCAGTATG ATAACGAAGC TGGGGAGCAG CCCACCTTCT CCAAATGCAG CTGGGGAAAG 
ACCACCAGTT CAGAACAGAC TCCAGGCCTG GCGCCCTTTC TCTCCCCAGA CAACACCAAA 
GAGGCAGTGA TCACCACGTA TCCCCCAGAC CTGGATGTGA TCGATGGCAG GATCTTCTCT 
CCAACCAACA GTGGCAGTGA ATCCATCTCT CCCACCCCTG CATTTCCTGT GTCTCCAGAA 
ACGCCGTATG GGACAACAAG CCCACGTTAC TCTCCGTTCA GCCCATCCGT GCCACAGATG 
GGCAGCCCCA GTGCTCTGTA TAAAGGAGCT CATGAACCCC GAGGCTGCCC TGAGATTCTC 
AGTCATTCTG TGGGGATGTC AGAGAGCCCT GTTGGACCCA AACCCACGAT GCTCCGGGCC 
GACATGCCCA CGGCGCCCTC CTTCCAGCGG GCATTCACGT CCTCCTGCAC CATTTCTGGG 
AACAGCCCCA GCCAGAGGAG AGGGAGCCCT TCCTCAGCTG AGCACCAGTG GGTGGAGACC 
AGCCCCAAGT CCACGCTGAC CCTCCTGGGG GGCAGCCGGC CTGGCAAGGA CAGCCCCGCG 
CGACCCCACT TCCCGCCTGC CGACCTCCAG ACATCCTTCC ACGGCCCCGA GCTGTCCCTG 
GCGGAGCCGC CGGAAGCTCT GGGTCCCCCG AGCAACCACG CCTTCCTGAG CTTCAGCACT 
GCCCCCATGG CGGGAGGTGG GCTCCCCGCC GGGGAGGACC CCGGGGCCTT GCTGGCCAAT 
TCCCACGGAG CAGCCCAGGC CCCTGGCAAC CCACTGACGG CAGCTGAGGC TGCCGACAAC 
AGCTTCCTGT CCCACAGCTT TCTCACGGTG GCACCTGGAC ACAGCGGCCA CCACAGCCCG 
GTCCTGCAGG GCCCGGGACT GGCTCTGCCC GGGCAGCCAC CCCTTCCTGA GAAGAAGCGG 
ACCTCAGAGG GAGATCGGTC TTTTGGCTCC GTCTCGCCAT CCTCCAGTGG CTTCTCCAGT 
CCTCACAGCG GGAGTACCAT GAGCATCCCT TTTCCAAACA TCATCCCGGA CTTTTCCAAG 
GCTGCCGAGG GGGCAGCACC TTCCCCAGAT AATCCAGGTG ATAAGCACGT GTCTGTAAAC 
TTTGTTCAGG ACACATCCAA GTTCTGGTAC AAGGCGGACA TCTCCAGAGA GCAAGCCATC 
GCCATGCTGA AGGACAAGGA GCCCGGGTCA TTCATCGTCA GGGACAGCCA CTCCTTCCGA 
GGTGCCTACG GCCTGGCCAT GAAGGTGGCC ACACCCCCGC CCTCTGTCCT GCAGATGAAC 
AAGAAAGCTG GAGATTTGGC CAATGAACTT GTTCGGCATT TTTTGATTGA GTGTACCCCA 
AAAGGAGTGC GGTTGAAAGG GTGCTCCAAT GAACCATATT TCGGGAGCCT GACGGCTTTG 
GTGTGTCAGC ATTCCATTAC GCCCCTGGCT TTGCCCTGCA AACTGCTCAT TCCGGATAGA 
GATCCATTGG AGGAAATAGC CGAAAATCCT CAGCAAACAG CGGCCAACTC TGCGGCCGAG 
CTGCTGAAGC AAGGGGCAGC CTGCAATGTG TGGTACCTGA ACTCCGTGGA GATGGAGTCC 
CTCACGGGCC ACCAAGCCAT CCAGAAGGCC CTGAGCATGA CCCTAGTCCA GGAGCCACCT 
CCCTTGTCCA CAGTCGTGCA CTTCAAGGTG TCGGCCCAGG GCATCACCCT GACGGACAAC 
CAGAGGAAGC TCTTCTTCCG GAGACACTAC CCCGTGAGCA GCGTGATCTT CTGTGCGCTG 
GACCCACAGG ACAGAAAGTG GGTCACAGAC GGCCCTTGCT CGAGAGTCTT TGGATTTGTG 
GCCCGGAAGC AGGGCAGTGC CACGGACAAC GTGTGCCACC TGTTTGCAGA GCATGACCCC 
GAGCAGCCGG CCAGCGCCAT TGTCAACTTC GTGTCAAAGG TCATGATCGG CTCCCCTAAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010804394.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802696.2 Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

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CloneID	OBa89746
Clone ID Related Accession (Same CDS sequence)	XM_010804394.2 , XM_010827363.2
Accession Version	XM_010804394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGA ACCCCGAGGC 
TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG ATGTCAGAGA GCCCTGTTGG ACCCAAACCC 
ACGATGCTCC GGGCCGACAT GCCCACGGCG CCCTCCTTCC AGCGGGCATT CACGTCCTCC 
TGCACCATTT CTGGGAACAG CCCCAGCCAG AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC 
CAGTGGGTGG AGACCAGCCC CAAGTCCACG CTGACCCTCC TGGGGGGCAG CCGGCCTGGC 
AAGGACAGCC CCGCGCGACC CCACTTCCCG CCTGCCGACC TCCAGACATC CTTCCACGGC 
CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA GCTCTGGGTC CCCCGAGCAA CCACGCCTTC 
CTGAGCTTCA GCACTGCCCC CATGGCGGGA GGTGGGCTCC CCGCCGGGGA GGACCCCGGG 
GCCTTGCTGG CCAATTCCCA CGGAGCAGCC CAGGCCCCTG GCAACCCACT GACGGCAGCT 
GAGGCTGCCG ACAACAGCTT CCTGTCCCAC AGCTTTCTCA CGGTGGCACC TGGACACAGC 
GGCCACCACA GCCCGGTCCT GCAGGGCCCG GGACTGGCTC TGCCCGGGCA GCCACCCCTT 
CCTGAGAAGA AGCGGACCTC AGAGGGAGAT CGGTCTTTTG GCTCCGTCTC GCCATCCTCC 
AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT ACCATGAGCA TCCCTTTTCC AAACATCATC 
CCGGACTTTT CCAAGGCTGC CGAGGGGGCA GCACCTTCCC CAGATAATCC AGGTGATAAG 
CACGTGTCTG TAAACTTTGT TCAGGACACA TCCAAGTTCT GGTACAAGGC GGACATCTCC 
AGAGAGCAAG CCATCGCCAT GCTGAAGGAC AAGGAGCCCG GGTCATTCAT CGTCAGGGAC 
AGCCACTCCT TCCGAGGTGC CTACGGCCTG GCCATGAAGG TGGCCACACC CCCGCCCTCT 
GTCCTGCAGA TGAACAAGAA AGCTGGAGAT TTGGCCAATG AACTTGTTCG GCATTTTTTG 
ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG AAAGGGTGCT CCAATGAACC ATATTTCGGG 
AGCCTGACGG CTTTGGTGTG TCAGCATTCC ATTACGCCCC TGGCTTTGCC CTGCAAACTG 
CTCATTCCGG ATAGAGATCC ATTGGAGGAA ATAGCCGAAA ATCCTCAGCA AACAGCGGCC 
AACTCTGCGG CCGAGCTGCT GAAGCAAGGG GCAGCCTGCA ATGTGTGGTA CCTGAACTCC 
GTGGAGATGG AGTCCCTCAC GGGCCACCAA GCCATCCAGA AGGCCCTGAG CATGACCCTA 
GTCCAGGAGC CACCTCCCTT GTCCACAGTC GTGCACTTCA AGGTGTCGGC CCAGGGCATC 
ACCCTGACGG ACAACCAGAG GAAGCTCTTC TTCCGGAGAC ACTACCCCGT GAGCAGCGTG 
ATCTTCTGTG CGCTGGACCC ACAGGACAGA AAGTGGGTCA CAGACGGCCC TTGCTCGAGA 
GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC AGTGCCACGG ACAACGTGTG CCACCTGTTT 
GCAGAGCATG ACCCCGAGCA GCCGGCCAGC GCCATTGTCA ACTTCGTGTC AAAGGTCATG 
ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802696.2
CDS	2181..6884
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010804394.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804394.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGA ACCCCGAGGC 
TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG ATGTCAGAGA GCCCTGTTGG ACCCAAACCC 
ACGATGCTCC GGGCCGACAT GCCCACGGCG CCCTCCTTCC AGCGGGCATT CACGTCCTCC 
TGCACCATTT CTGGGAACAG CCCCAGCCAG AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC 
CAGTGGGTGG AGACCAGCCC CAAGTCCACG CTGACCCTCC TGGGGGGCAG CCGGCCTGGC 
AAGGACAGCC CCGCGCGACC CCACTTCCCG CCTGCCGACC TCCAGACATC CTTCCACGGC 
CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA GCTCTGGGTC CCCCGAGCAA CCACGCCTTC 
CTGAGCTTCA GCACTGCCCC CATGGCGGGA GGTGGGCTCC CCGCCGGGGA GGACCCCGGG 
GCCTTGCTGG CCAATTCCCA CGGAGCAGCC CAGGCCCCTG GCAACCCACT GACGGCAGCT 
GAGGCTGCCG ACAACAGCTT CCTGTCCCAC AGCTTTCTCA CGGTGGCACC TGGACACAGC 
GGCCACCACA GCCCGGTCCT GCAGGGCCCG GGACTGGCTC TGCCCGGGCA GCCACCCCTT 
CCTGAGAAGA AGCGGACCTC AGAGGGAGAT CGGTCTTTTG GCTCCGTCTC GCCATCCTCC 
AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT ACCATGAGCA TCCCTTTTCC AAACATCATC 
CCGGACTTTT CCAAGGCTGC CGAGGGGGCA GCACCTTCCC CAGATAATCC AGGTGATAAG 
CACGTGTCTG TAAACTTTGT TCAGGACACA TCCAAGTTCT GGTACAAGGC GGACATCTCC 
AGAGAGCAAG CCATCGCCAT GCTGAAGGAC AAGGAGCCCG GGTCATTCAT CGTCAGGGAC 
AGCCACTCCT TCCGAGGTGC CTACGGCCTG GCCATGAAGG TGGCCACACC CCCGCCCTCT 
GTCCTGCAGA TGAACAAGAA AGCTGGAGAT TTGGCCAATG AACTTGTTCG GCATTTTTTG 
ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG AAAGGGTGCT CCAATGAACC ATATTTCGGG 
AGCCTGACGG CTTTGGTGTG TCAGCATTCC ATTACGCCCC TGGCTTTGCC CTGCAAACTG 
CTCATTCCGG ATAGAGATCC ATTGGAGGAA ATAGCCGAAA ATCCTCAGCA AACAGCGGCC 
AACTCTGCGG CCGAGCTGCT GAAGCAAGGG GCAGCCTGCA ATGTGTGGTA CCTGAACTCC 
GTGGAGATGG AGTCCCTCAC GGGCCACCAA GCCATCCAGA AGGCCCTGAG CATGACCCTA 
GTCCAGGAGC CACCTCCCTT GTCCACAGTC GTGCACTTCA AGGTGTCGGC CCAGGGCATC 
ACCCTGACGG ACAACCAGAG GAAGCTCTTC TTCCGGAGAC ACTACCCCGT GAGCAGCGTG 
ATCTTCTGTG CGCTGGACCC ACAGGACAGA AAGTGGGTCA CAGACGGCCC TTGCTCGAGA 
GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC AGTGCCACGG ACAACGTGTG CCACCTGTTT 
GCAGAGCATG ACCCCGAGCA GCCGGCCAGC GCCATTGTCA ACTTCGTGTC AAAGGTCATG 
ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_010827363.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825665.2 Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

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CloneID	OBa89746
Clone ID Related Accession (Same CDS sequence)	XM_010804394.2 , XM_010827363.2
Accession Version	XM_010827363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGA ACCCCGAGGC 
TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG ATGTCAGAGA GCCCTGTTGG ACCCAAACCC 
ACGATGCTCC GGGCCGACAT GCCCACGGCG CCCTCCTTCC AGCGGGCATT CACGTCCTCC 
TGCACCATTT CTGGGAACAG CCCCAGCCAG AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC 
CAGTGGGTGG AGACCAGCCC CAAGTCCACG CTGACCCTCC TGGGGGGCAG CCGGCCTGGC 
AAGGACAGCC CCGCGCGACC CCACTTCCCG CCTGCCGACC TCCAGACATC CTTCCACGGC 
CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA GCTCTGGGTC CCCCGAGCAA CCACGCCTTC 
CTGAGCTTCA GCACTGCCCC CATGGCGGGA GGTGGGCTCC CCGCCGGGGA GGACCCCGGG 
GCCTTGCTGG CCAATTCCCA CGGAGCAGCC CAGGCCCCTG GCAACCCACT GACGGCAGCT 
GAGGCTGCCG ACAACAGCTT CCTGTCCCAC AGCTTTCTCA CGGTGGCACC TGGACACAGC 
GGCCACCACA GCCCGGTCCT GCAGGGCCCG GGACTGGCTC TGCCCGGGCA GCCACCCCTT 
CCTGAGAAGA AGCGGACCTC AGAGGGAGAT CGGTCTTTTG GCTCCGTCTC GCCATCCTCC 
AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT ACCATGAGCA TCCCTTTTCC AAACATCATC 
CCGGACTTTT CCAAGGCTGC CGAGGGGGCA GCACCTTCCC CAGATAATCC AGGTGATAAG 
CACGTGTCTG TAAACTTTGT TCAGGACACA TCCAAGTTCT GGTACAAGGC GGACATCTCC 
AGAGAGCAAG CCATCGCCAT GCTGAAGGAC AAGGAGCCCG GGTCATTCAT CGTCAGGGAC 
AGCCACTCCT TCCGAGGTGC CTACGGCCTG GCCATGAAGG TGGCCACACC CCCGCCCTCT 
GTCCTGCAGA TGAACAAGAA AGCTGGAGAT TTGGCCAATG AACTTGTTCG GCATTTTTTG 
ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG AAAGGGTGCT CCAATGAACC ATATTTCGGG 
AGCCTGACGG CTTTGGTGTG TCAGCATTCC ATTACGCCCC TGGCTTTGCC CTGCAAACTG 
CTCATTCCGG ATAGAGATCC ATTGGAGGAA ATAGCCGAAA ATCCTCAGCA AACAGCGGCC 
AACTCTGCGG CCGAGCTGCT GAAGCAAGGG GCAGCCTGCA ATGTGTGGTA CCTGAACTCC 
GTGGAGATGG AGTCCCTCAC GGGCCACCAA GCCATCCAGA AGGCCCTGAG CATGACCCTA 
GTCCAGGAGC CACCTCCCTT GTCCACAGTC GTGCACTTCA AGGTGTCGGC CCAGGGCATC 
ACCCTGACGG ACAACCAGAG GAAGCTCTTC TTCCGGAGAC ACTACCCCGT GAGCAGCGTG 
ATCTTCTGTG CGCTGGACCC ACAGGACAGA AAGTGGGTCA CAGACGGCCC TTGCTCGAGA 
GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC AGTGCCACGG ACAACGTGTG CCACCTGTTT 
GCAGAGCATG ACCCCGAGCA GCCGGCCAGC GCCATTGTCA ACTTCGTGTC AAAGGTCATG 
ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825665.2
CDS	2181..6884
Translation	MSPQGQAGPLAGASAFCTHSSRTTTTTGLRRWLREQKGSIDSAPLVIHHLSQRFPECSPLTTGDFGRTSKPEEVTASLHSFKNKAFKKARVCGVCKQIIEGRGISCRACKYSCHKKCEAKVVSPHCVQVHLEQAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_010827363.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827363.2

ORF Insert Sequence:

ATGTCGCCCC AGGGCCAGGC TGGGCCATTA GCTGGGGCGT CTGCGTTCTG TACACATTCC 
TCCCGGACGA CGACGACGAC GGGGCTGCGG CGCTGGCTGC GGGAACAAAA GGGCTCCATT 
GACTCTGCTC CGCTCGTAAT CCATCACCTT TCTCAGAGGT TTCCTGAGTG CTCACCCCTC 
ACCACTGGTG ATTTTGGACG CACATCAAAG CCAGAAGAAG TAACTGCGAG TCTGCACAGT 
TTTAAGAACA AGGCCTTTAA AAAAGCCAGA GTCTGTGGGG TTTGCAAACA GATTATTGAA 
GGTCGAGGGA TTTCATGCCG AGCCTGCAAG TATTCCTGTC ACAAGAAATG TGAAGCCAAG 
GTGGTGAGTC CCCACTGTGT GCAGGTCCAC CTGGAACAGG CTCCGGGGGC ATCATCAACA 
CCTGCTTCTC TGTGCTGTGA CAAACCGTTC AGACCTGCAG GGCTGAGCCC CGCCATGGAG 
GACGGCCGCG AGCTGGATCT CACCTACATC ACCGAGCGCA TCATTGCCGT GTCCTTCCCC 
GCGGGCTGCT CCGAGGAGTC TTACCTGCAC AACCTGCAGG AGGTGACGCG CATGCTACGC 
TCCAAGCATG GGGACAACTA CTTGGTGTTA AACCTCTCGG AAAAGAGATA TGACCTTACA 
AAGCTTAACC CAAAGATTCT GGACGTGGGC TGGCCGGAGC TGCATGCGCC ACCCCTGGAT 
AAGGTGTGCA CCATCTGCAA GGCGCAGGAG GCCTGGCTGA ACAGCGACCC CCAACACGTG 
GTTGTTATTC ACTGCAGGGG TGGAAAGGGA CGCATCGGCG TGGTCATATC ATCTTACATG 
CACTTCACCA ATGTCTCGGC CAGTGCTGAC CAGGCCCTTG ACAGATTCGC AATGAAGAAG 
TTTTATGATG ATAAACTTGC AGCTTTAATG CAGCCATCCC AGAAACGGTA TGTGCAGTTT 
CTCAGTGGGC TCCTGTCAGG AACGGTGAAG ATGAATGCCT CGCCCCTGTT CCTGCATTTC 
GTCATCCTGC ACGGAACCCC CAATTTTGAC TCCGGTGGAG TGTGCCGGCC CTTTCTCAAG 
CTCTACCAGG CCATGCAGCC CGTGTACACG TCGGGGATCT ACAATGTTGG CCCAGAAAAC 
CAAAGCAGGA TCTACATTGC CATCGAGCCG GCCCAGCTCC TGAAAGGGGA CATCATGGTG 
AAGTGCTACC ACAAGAAGTA CCGCTCGGCC ACCCGTGATG TCATTTTCCG CCTGCAGTTT 
CACACCGGGG CTGTTCAGGG CTACAGACTT GTGTTTGCGA AGGAGGATTT GGACAACGCG 
AGCAAAGATG ACCGTTTTCC TGATAATGGG AAGATTGAGT TAGTCTTCTC AGCCACTCCT 
GAGAAGATTC AAGGATGTGA ACATTTGCAG AATGACCACA GAGTGATTGT GGACTTCAAC 
ACAGCAGACC CGCTAATCCG CTGGGACTCC TACGAGAACC TGAGTGCAGA CGGTGAAGTG 
CTGCACACGC AGGGCCCCGT GGACGGCAGT CTTTACGCCA AGGTGAGAAA GAAGAGCACT 
TCAGATCCCA GTGTCCCAGG CGGGTCCCCC GCTGTCCCAG CCGCCAGCAG CCCAGACCAC 
AGCGATCACA CCCTGTCTGT CAGCAGTGAC TCGGGCCACT CCACAGCCTC TGTGAGGACC 
GACAGGACCG AGGAGCACCT GACTCCCGGA TCCAAGAGGG GCCTGAGCCC CCAGGAAAAG 
GCCGAGCTGG ATCAGTTGCT CAGTGGCTTT GGCCTCGAGG ATTCTGGAAG CCCCCTGAAG 
GATATGAGCG ATGCTCGCAG CAAGTACAGC GGGACCCGCC ACGTTGTACC GGCCCAGGTC 
CACGTGAACG GAGACACCAC CCCCAAGGAC CGAGAGACGG ACATCCTGGA TGACGAGATG 
CCCAGCCACG ACCTGCACAG CGTGGACAGC ATCGGGACCC TGTCCTCCTC GGAGGGCCAC 
CCGTCAGCCC ACCTGAGCCC CTTCACCTGC CACCAGAGCA GCCAGAACTC CCTCCTGTCT 
GATGGATTCG GCAGCACTGC CGGCGAAGAT CAGCATGGTG CTCTGGCCCC AGATCTGGGG 
CTCGCCGCGG AGCCCCTGTT CGAGCGAGAG CGGGCTTTCG GGAGCTGCGA GCCCAAGCAG 
CCACAGCCTG TGCTCAGGAA GCCCTCTGTG CCTGCCCAGA CGCAGGCCTA CGGGCCGAGC 
AGCTACTCCA CGCAGACCTG GGTGCGCCAG CAGCAGATGG TGGCCGCTCA CCAGTACAGC 
TTTGCCCCTG ATGGGGAGGC CAGGCTCAGC GGCCGTGGTG CAGCAGACAG TTCTGGCCTG 
CTGCAGACAC AGCCTAGGGT CCCGCTCACC CCCACTCGGG GGGCCAGCAG CAGAGTGGCC 
GTCCAGAGGG GCATAGGCCC TGGGCCACAT CCTCCCGATA TACAGAGACC CCCTCCTGGC 
AAAGCAGCCT TCAAACCCAG GATTCCAGAT GTCAAAGTCG TGAATGGTGC TGGCCCAGAG 
CTGGGCACAG ACCTGTCCCC AGGCTCACCC ACCCTGGACA TCGACCAGTC GATCGAACAG 
CTCAACAGGC TGATCTTGGA GCTGGACCCC ACCTTTGAGC CCATCCCCAC CCACATGAAC 
ATGTCGAGCA GCCAGGCCAA CGGCCCCATG CCCCCAGACG GCATGGCAGG CGGGCTATGG 
GCCAGTGGCC GCCTGCAGGA CACAGGAGAT GGCCCTGGCA GGGCCCCAGT GAGGCAAGGG 
GATGAGCCAC TTGGGGGAAG ATTCCGGAAG CTAAGCCTTG AGCAGTATGA TAACGAAGCT 
GGGGAGCAGC CCACCTTCTC CAAATGCAGC TGGGGAAAGA CCACCAGTTC AGAACAGACT 
CCAGGCCTGG CGCCCTTTCT CTCCCCAGAC AACACCAAAG AGGCAGTGAT CACCACGTAT 
CCCCCAGACC TGGATGTGAT CGATGGCAGG ATCTTCTCTC CAACCAACAG TGGCAGTGAA 
TCCATCTCTC CCACCCCTGC ATTTCCTGTG TCTCCAGAAA CGCCGTATGA ACCCCGAGGC 
TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG ATGTCAGAGA GCCCTGTTGG ACCCAAACCC 
ACGATGCTCC GGGCCGACAT GCCCACGGCG CCCTCCTTCC AGCGGGCATT CACGTCCTCC 
TGCACCATTT CTGGGAACAG CCCCAGCCAG AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC 
CAGTGGGTGG AGACCAGCCC CAAGTCCACG CTGACCCTCC TGGGGGGCAG CCGGCCTGGC 
AAGGACAGCC CCGCGCGACC CCACTTCCCG CCTGCCGACC TCCAGACATC CTTCCACGGC 
CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA GCTCTGGGTC CCCCGAGCAA CCACGCCTTC 
CTGAGCTTCA GCACTGCCCC CATGGCGGGA GGTGGGCTCC CCGCCGGGGA GGACCCCGGG 
GCCTTGCTGG CCAATTCCCA CGGAGCAGCC CAGGCCCCTG GCAACCCACT GACGGCAGCT 
GAGGCTGCCG ACAACAGCTT CCTGTCCCAC AGCTTTCTCA CGGTGGCACC TGGACACAGC 
GGCCACCACA GCCCGGTCCT GCAGGGCCCG GGACTGGCTC TGCCCGGGCA GCCACCCCTT 
CCTGAGAAGA AGCGGACCTC AGAGGGAGAT CGGTCTTTTG GCTCCGTCTC GCCATCCTCC 
AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT ACCATGAGCA TCCCTTTTCC AAACATCATC 
CCGGACTTTT CCAAGGCTGC CGAGGGGGCA GCACCTTCCC CAGATAATCC AGGTGATAAG 
CACGTGTCTG TAAACTTTGT TCAGGACACA TCCAAGTTCT GGTACAAGGC GGACATCTCC 
AGAGAGCAAG CCATCGCCAT GCTGAAGGAC AAGGAGCCCG GGTCATTCAT CGTCAGGGAC 
AGCCACTCCT TCCGAGGTGC CTACGGCCTG GCCATGAAGG TGGCCACACC CCCGCCCTCT 
GTCCTGCAGA TGAACAAGAA AGCTGGAGAT TTGGCCAATG AACTTGTTCG GCATTTTTTG 
ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG AAAGGGTGCT CCAATGAACC ATATTTCGGG 
AGCCTGACGG CTTTGGTGTG TCAGCATTCC ATTACGCCCC TGGCTTTGCC CTGCAAACTG 
CTCATTCCGG ATAGAGATCC ATTGGAGGAA ATAGCCGAAA ATCCTCAGCA AACAGCGGCC 
AACTCTGCGG CCGAGCTGCT GAAGCAAGGG GCAGCCTGCA ATGTGTGGTA CCTGAACTCC 
GTGGAGATGG AGTCCCTCAC GGGCCACCAA GCCATCCAGA AGGCCCTGAG CATGACCCTA 
GTCCAGGAGC CACCTCCCTT GTCCACAGTC GTGCACTTCA AGGTGTCGGC CCAGGGCATC 
ACCCTGACGG ACAACCAGAG GAAGCTCTTC TTCCGGAGAC ACTACCCCGT GAGCAGCGTG 
ATCTTCTGTG CGCTGGACCC ACAGGACAGA AAGTGGGTCA CAGACGGCCC TTGCTCGAGA 
GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC AGTGCCACGG ACAACGTGTG CCACCTGTTT 
GCAGAGCATG ACCCCGAGCA GCCGGCCAGC GCCATTGTCA ACTTCGTGTC AAAGGTCATG 
ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_594710.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_594710.8 Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa89747
Clone ID Related Accession (Same CDS sequence)	XM_594710.9 , XM_005205611.3
Accession Version	XM_594710.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_594710.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG ACCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATTGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ATTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCGGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTCT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCACGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAGCC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_594710.8
CDS	25..4560
Translation	MQRSFCSFLRDLFTAPVSGLVSMAGSSTQVRRLWEAGGREVSSGPAKFLAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_594710.9
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_594710.9

ORF Insert Sequence:

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG ACCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATTGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ATTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCGGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTCT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCACGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAGCC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_005205611.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205668.3 Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa89747
Clone ID Related Accession (Same CDS sequence)	XM_594710.9 , XM_005205611.3
Accession Version	XM_005205611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG ACCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATTGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ATTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCGGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTCT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCACGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAGCC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205668.3
CDS	25..4560
Translation	MQRSFCSFLRDLFTAPVSGLVSMAGSSTQVRRLWEAGGREVSSGPAKFLAPGASSTPASLCCDKPFRPAGLSPAMEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_005205611.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205611.3

ORF Insert Sequence:

ATGCAACGTT CCTTTTGTTC CTTTTTGCGG GACCTTTTCA CAGCGCCAGT TTCAGGGCTG 
GTCAGCATGG CTGGGAGCAG CACACAGGTC CGACGACTCT GGGAAGCTGG GGGCCGTGAA 
GTGTCCTCGG GCCCTGCCAA GTTTCTGGCT CCGGGGGCAT CATCAACACC TGCTTCTCTG 
TGCTGTGACA AACCGTTCAG ACCTGCAGGG CTGAGCCCCG CCATGGAGGA CGGCCGCGAG 
CTGGATCTCA CCTACATCAC CGAGCGCATC ATTGCCGTGT CCTTCCCCGC GGGCTGCTCC 
GAGGAGTCTT ACCTGCACAA CCTGCAGGAG GTGACGCGCA TGCTACGCTC CAAGCATGGG 
GACAACTACT TGGTGTTAAA CCTCTCGGAA AAGAGATATG ACCTTACAAA GCTTAACCCA 
AAGATTCTGG ACGTGGGCTG GCCGGAGCTG CATGCGCCAC CCCTGGATAA GGTGTGCACC 
ATCTGCAAGG CGCAGGAGGC CTGGCTGAAC AGCGACCCCC AACACGTGGT TGTTATTCAC 
TGCAGGGGTG GAAAGGGACG CATCGGCGTG GTCATATCAT CTTACATGCA CTTCACCAAT 
GTCTCGGCCA GTGCTGACCA GGCCCTTGAC AGATTCGCAA TGAAGAAGTT TTATGATGAT 
AAACTTGCAG CTTTAATGCA GCCATCCCAG AAACGGTATG TGCAGTTTCT CAGTGGGCTC 
CTGTCAGGAA CGGTGAAGAT GAATGCCTCG CCCCTGTTCC TGCATTTCGT CATCCTGCAC 
GGAACCCCCA ATTTTGACTC CGGTGGAGTG TGCCGGCCCT TTCTCAAGCT CTACCAGGCC 
ATGCAGCCCG TGTACACGTC GGGGATCTAC AATGTTGGCC CAGAAAACCA AAGCAGGATC 
TACATTGCCA TCGAGCCGGC CCAGCTCCTG AAAGGGGACA TCATGGTGAA GTGCTACCAC 
AAGAAGTACC GCTCGGCCAC CCGTGATGTC ATTTTCCGCC TGCAGTTTCA CACCGGGGCT 
GTTCAGGGCT ACAGACTTGT GTTTGCGAAG GAGGATTTGG ACAACGCGAG CAAAGATGAC 
CGTTTTCCTG ATAATGGGAA GATTGAGTTA GTCTTCTCAG CCACTCCTGA GAAGATTCAA 
GGATGTGAAC ATTTGCAGAA TGACCACAGA GTGATTGTGG ACTTCAACAC AGCAGACCCG 
CTAATCCGCT GGGACTCCTA CGAGAACCTG AGTGCAGACG GTGAAGTGCT GCACACGCAG 
GGCCCCGTGG ACGGCAGTCT TTACGCCAAG GTGAGAAAGA AGAGCACTTC AGATCCCAGT 
GTCCCAGGCG GGTCCCCCGC TGTCCCAGCC GCCAGCAGCC CAGACCACAG CGATCACACC 
CTGTCTGTCA GCAGTGACTC GGGCCACTCC ACAGCCTCTG TGAGGACCGA CAGGACCGAG 
GAGCACCTGA CTCCCGGATC CAAGAGGGGC CTGAGCCCCC AGGAAAAGGC CGAGCTGGAT 
CAGTTGCTCA GTGGCTTTGG CCTCGAGGAT TCTGGAAGCC CCCTGAAGGA TATGAGCGAT 
GCTCGCAGCA AGTACAGCGG GACCCGCCAC GTTGTACCGG CCCAGGTCCA CGTGAACGGA 
GACACCACCC CCAAGGACCG AGAGACGGAC ATCCTGGATG ACGAGATGCC CAGCCACGAC 
CTGCACAGCG TGGACAGCAT CGGGACCCTG TCCTCCTCGG AGGGCCACCC GTCAGCCCAC 
CTGAGCCCCT TCACCTGCCA CCAGAGCAGC CAGAACTCCC TCCTGTCTGA TGGATTCGGC 
AGCACTGCCG GCGAAGATCA GCATGGTGCT CTGGCCCCAG ATCTGGGGCT CGCCGCGGAG 
CCCCTGTTCG AGCGAGAGCG GGCTTTCGGG AGCTGCGAGC CCAAGCAGCC ACAGCCTGTG 
CTCAGGAAGC CCTCTGTGCC TGCCCAGACG CAGGCCTACG GGCCGAGCAG CTACTCCACG 
CAGACCTGGG TGCGCCAGCA GCAGATGGTG GCCGCTCACC AGTACAGCTT TGCCCCTGAT 
GGGGAGGCCA GGCTCAGCGG CCGTGGTGCA GCAGACAGTT CTGGCCTGCT GCAGACACAG 
CCTAGGGTCC CGCTCACCCC CACTCGGGGG GCCAGCAGCA GAGTGGCCGT CCAGAGGGGC 
ATAGGCCCTG GGCCACATCC TCCCGATATA CAGAGACCCC CTCCTGGCAA AGCAGCCTTC 
AAACCCAGGA TTCCAGATGT CAAAGTCGTG AATGGTGCTG GCCCAGAGCT GGGCACAGAC 
CTGTCCCCAG GCTCACCCAC CCTGGACATC GACCAGTCGA TCGAACAGCT CAACAGGCTG 
ATCTTGGAGC TGGACCCCAC CTTTGAGCCC ATCCCCACCC ACATGAACAT GTCGAGCAGC 
CAGGCCAACG GCCCCATGCC CCCAGACGGC ATGGCAGGCG GGCTATGGGC CAGTGGCCGC 
CTGCAGGACA CAGGAGATGG CCCTGGCAGG GCCCCAGTGA GGCAAGGGGA TGAGCCACTT 
GGGGGAAGAT TCCGGAAGCT AAGCCTTGAG CAGTATGATA ACGAAGCTGG GGAGCAGCCC 
ACCTTCTCCA AATGCAGCTG GGGAAAGACC ACCAGTTCAG AACAGACTCC AGGCCTGGCG 
CCCTTTCTCT CCCCAGACAA CACCAAAGAG GCAGTGATCA CCACGTATCC CCCAGACCTG 
GATGTGATCG ATGGCAGGAT CTTCTCTCCA ACCAACAGTG GCAGTGAATC CATCTCTCCC 
ACCCCTGCAT TTCCTGTGTC TCCAGAAACG CCGTATGGGA CAACAAGCCC ACGTTACTCT 
CCGTTCAGCC CATCCGTGCC ACAGATGGGC AGCCCCAGTG CTCTGTATAA AGGAGCTCAT 
GAACCCCGAG GCTGCCCTGA GATTCTCAGT CATTCTGTGG GGATGTCAGA GAGCCCTGTT 
GGACCCAAAC CCACGATGCT CCGGGCCGAC ATGCCCACGG CGCCCTCCTT CCAGCGGGCA 
TTCACGTCCT CCTGCACCAT TTCTGGGAAC AGCCCCAGCC AGAGGAGAGG GAGCCCTTCC 
TCAGCTGAGC ACCAGTGGGT GGAGACCAGC CCCAAGTCCA CGCTGACCCT CCTGGGGGGC 
AGCCGGCCTG GCAAGGACAG CCCCGCGCGA CCCCACTTCC CGCCTGCCGA CCTCCAGACA 
TCCTTCCACG GCCCCGAGCT GTCCCTGGCG GAGCCGCCGG AAGCTCTGGG TCCCCCGAGC 
AACCACGCCT TCCTGAGCTT CAGCACTGCC CCCATGGCGG GAGGTGGGCT CCCCGCCGGG 
GAGGACCCCG GGGCCTTGCT GGCCAATTCC CACGGAGCAG CCCAGGCCCC TGGCAACCCA 
CTGACGGCAG CTGAGGCTGC CGACAACAGC TTCCTGTCCC ACAGCTTTCT CACGGTGGCA 
CCTGGACACA GCGGCCACCA CAGCCCGGTC CTGCAGGGCC CGGGACTGGC TCTGCCCGGG 
CAGCCACCCC TTCCTGAGAA GAAGCGGACC TCAGAGGGAG ATCGGTCTTT TGGCTCCGTC 
TCGCCATCCT CCAGTGGCTT CTCCAGTCCT CACAGCGGGA GTACCATGAG CATCCCTTTT 
CCAAACATCA TCCCGGACTT TTCCAAGGCT GCCGAGGGGG CAGCACCTTC CCCAGATAAT 
CCAGGTGATA AGCACGTGTC TGTAAACTTT GTTCAGGACA CATCCAAGTT CTGGTACAAG 
GCGGACATCT CCAGAGAGCA AGCCATCGCC ATGCTGAAGG ACAAGGAGCC CGGGTCATTC 
ATCGTCAGGG ACAGCCACTC CTTCCGAGGT GCCTACGGCC TGGCCATGAA GGTGGCCACA 
CCCCCGCCCT CTGTCCTGCA GATGAACAAG AAAGCTGGAG ATTTGGCCAA TGAACTTGTT 
CGGCATTTTT TGATTGAGTG TACCCCAAAA GGAGTGCGGT TGAAAGGGTG CTCCAATGAA 
CCATATTTCG GGAGCCTGAC GGCTTTGGTG TGTCAGCATT CCATTACGCC CCTGGCTTTG 
CCCTGCAAAC TGCTCATTCC GGATAGAGAT CCATTGGAGG AAATAGCCGA AAATCCTCAG 
CAAACAGCGG CCAACTCTGC GGCCGAGCTG CTGAAGCAAG GGGCAGCCTG CAATGTGTGG 
TACCTGAACT CCGTGGAGAT GGAGTCCCTC ACGGGCCACC AAGCCATCCA GAAGGCCCTG 
AGCATGACCC TAGTCCAGGA GCCACCTCCC TTGTCCACAG TCGTGCACTT CAAGGTGTCG 
GCCCAGGGCA TCACCCTGAC GGACAACCAG AGGAAGCTCT TCTTCCGGAG ACACTACCCC 
GTGAGCAGCG TGATCTTCTG TGCGCTGGAC CCACAGGACA GAAAGTGGGT CACAGACGGC 
CCTTGCTCGA GAGTCTTTGG ATTTGTGGCC CGGAAGCAGG GCAGTGCCAC GGACAACGTG 
TGCCACCTGT TTGCAGAGCA TGACCCCGAG CAGCCGGCCA GCGCCATTGT CAACTTCGTG 
TCAAAGGTCA TGATCGGCTC CCCTAAGAAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015463074.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318560.1 Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.

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CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_015463074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318560.1
CDS	755..5068
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015463074.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463074.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_005198091.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198148.2 Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.

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CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_005198091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198148.2
CDS	361..4674
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_005198091.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198091.3

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015463075.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318561.1 Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_015463075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318561.1
CDS	170..4483
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015463075.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463075.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015470044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325530.1 Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_015470044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325530.1
CDS	170..4483
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015470044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470044.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_005205612.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205669.1 Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.

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CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_005205612.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205612.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205669.1
CDS	361..4674
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_005205612.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205612.3

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS3 ( XM_015470043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325529.1 Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.

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CloneID	OBa18699
Clone ID Related Accession (Same CDS sequence)	XM_015463074.1 , XM_005198091.3 , XM_015463075.1 , XM_015470044.1 , XM_005205612.3 , XM_015470043.1
Accession Version	XM_015470043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325529.1
CDS	755..5068
Translation	MEDGRELDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLRSKHGDNYLVLNLSEKRYDLTKLNPKILDVGWPELHAPPLDKVCTICKAQEAWLNSDPQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKLAALMQPSQKRYVQFLSGLLSGTVKMNASPLFLHFVILHGTPNFDSGGVCRPFLKLYQAMQPVYTSGIYNVGPENQSRIYIAIEPAQLLKGDIMVKCYHKKYRSATRDVIFRLQFHTGAVQGYRLVFAKEDLDNASKDDRFPDNGKIELVFSATPEKIQGCEHLQNDHRVIVDFNTADPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSTSDPSVPGGSPAVPAASSPDHSDHTLSVSSDSGHSTASVRTDRTEEHLTPGSKRGLSPQEKAELDQLLSGFGLEDSGSPLKDMSDARSKYSGTRHVVPAQVHVNGDTTPKDRETDILDDEMPSHDLHSVDSIGTLSSSEGHPSAHLSPFTCHQSSQNSLLSDGFGSTAGEDQHGALAPDLGLAAEPLFERERAFGSCEPKQPQPVLRKPSVPAQTQAYGPSSYSTQTWVRQQQMVAAHQYSFAPDGEARLSGRGAADSSGLLQTQPRVPLTPTRGASSRVAVQRGIGPGPHPPDIQRPPPGKAAFKPRIPDVKVVNGAGPELGTDLSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNMSSSQANGPMPPDGMAGGLWASGRLQDTGDGPGRAPVRQGDEPLGGRFRKLSLEQYDNEAGEQPTFSKCSWGKTTSSEQTPGLAPFLSPDNTKEAVITTYPPDLDVIDGRIFSPTNSGSESISPTPAFPVSPETPYGTTSPRYSPFSPSVPQMGSPSALYKGAHEPRGCPEILSHSVGMSESPVGPKPTMLRADMPTAPSFQRAFTSSCTISGNSPSQRRGSPSSAEHQWVETSPKSTLTLLGGSRPGKDSPARPHFPPADLQTSFHGPELSLAEPPEALGPPSNHAFLSFSTAPMAGGGLPAGEDPGALLANSHGAAQAPGNPLTAAEAADNSFLSHSFLTVAPGHSGHHSPVLQGPGLALPGQPPLPEKKRTSEGDRSFGSVSPSSSGFSSPHSGSTMSIPFPNIIPDFSKAAEGAAPSPDNPGDKHVSVNFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQMNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPDRDPLEEIAENPQQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSMTLVQEPPPLSTVVHFKVSAQGITLTDNQRKLFFRRHYPVSSVIFCALDPQDRKWVTDGPCSRVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKI

Target ORF information:

RefSeq Version	XM_015470043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 3 (TNS3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470043.1

ORF Insert Sequence:

ATGGAGGACG GCCGCGAGCT GGATCTCACC TACATCACCG AGCGCATCAT TGCCGTGTCC 
TTCCCCGCGG GCTGCTCCGA GGAGTCTTAC CTGCACAACC TGCAGGAGGT GACGCGCATG 
CTACGCTCCA AGCATGGGGA CAACTACTTG GTGTTAAACC TCTCGGAAAA GAGATATGAC 
CTTACAAAGC TTAACCCAAA GATTCTGGAC GTGGGCTGGC CGGAGCTGCA TGCGCCACCC 
CTGGATAAGG TGTGCACCAT CTGCAAGGCG CAGGAGGCCT GGCTGAACAG CGACCCCCAA 
CACGTGGTTG TTATTCACTG CAGGGGTGGA AAGGGACGCA TCGGCGTGGT CATATCATCT 
TACATGCACT TCACCAATGT CTCGGCCAGT GCTGACCAGG CCCTTGACAG ATTCGCAATG 
AAGAAGTTTT ATGATGATAA ACTTGCAGCT TTAATGCAGC CATCCCAGAA ACGGTATGTG 
CAGTTTCTCA GTGGGCTCCT GTCAGGAACG GTGAAGATGA ATGCCTCGCC CCTGTTCCTG 
CATTTCGTCA TCCTGCACGG AACCCCCAAT TTTGACTCCG GTGGAGTGTG CCGGCCCTTT 
CTCAAGCTCT ACCAGGCCAT GCAGCCCGTG TACACGTCGG GGATCTACAA TGTTGGCCCA 
GAAAACCAAA GCAGGATCTA CATTGCCATC GAGCCGGCCC AGCTCCTGAA AGGGGACATC 
ATGGTGAAGT GCTACCACAA GAAGTACCGC TCGGCCACCC GTGATGTCAT TTTCCGCCTG 
CAGTTTCACA CCGGGGCTGT TCAGGGCTAC AGACTTGTGT TTGCGAAGGA GGATTTGGAC 
AACGCGAGCA AAGATGACCG TTTTCCTGAT AATGGGAAGA TTGAGTTAGT CTTCTCAGCC 
ACTCCTGAGA AGATTCAAGG ATGTGAACAT TTGCAGAATG ACCACAGAGT GATTGTGGAC 
TTCAACACAG CAGACCCGCT AATCCGCTGG GACTCCTACG AGAACCTGAG TGCAGACGGT 
GAAGTGCTGC ACACGCAGGG CCCCGTGGAC GGCAGTCTTT ACGCCAAGGT GAGAAAGAAG 
AGCACTTCAG ATCCCAGTGT CCCAGGCGGG TCCCCCGCTG TCCCAGCCGC CAGCAGCCCA 
GACCACAGCG ATCACACCCT GTCTGTCAGC AGTGACTCGG GCCACTCCAC AGCCTCTGTG 
AGGACCGACA GGACCGAGGA GCACCTGACT CCCGGATCCA AGAGGGGCCT GAGCCCCCAG 
GAAAAGGCCG AGCTGGATCA GTTGCTCAGT GGCTTTGGCC TCGAGGATTC TGGAAGCCCC 
CTGAAGGATA TGAGCGATGC TCGCAGCAAG TACAGCGGGA CCCGCCACGT TGTACCGGCC 
CAGGTCCACG TGAACGGAGA CACCACCCCC AAGGACCGAG AGACGGACAT CCTGGATGAC 
GAGATGCCCA GCCACGACCT GCACAGCGTG GACAGCATCG GGACCCTGTC CTCCTCGGAG 
GGCCACCCGT CAGCCCACCT GAGCCCCTTC ACCTGCCACC AGAGCAGCCA GAACTCCCTC 
CTGTCTGATG GATTCGGCAG CACTGCCGGC GAAGATCAGC ATGGTGCTCT GGCCCCAGAT 
CTGGGGCTCG CCGCGGAGCC CCTGTTCGAG CGAGAGCGGG CTTTCGGGAG CTGCGAGCCC 
AAGCAGCCAC AGCCTGTGCT CAGGAAGCCC TCTGTGCCTG CCCAGACGCA GGCCTACGGG 
CCGAGCAGCT ACTCCACGCA GACCTGGGTG CGCCAGCAGC AGATGGTGGC CGCTCACCAG 
TACAGCTTTG CCCCTGATGG GGAGGCCAGG CTCAGCGGCC GTGGTGCAGC AGACAGTTCT 
GGCCTGCTGC AGACACAGCC TAGGGTCCCG CTCACCCCCA CTCGGGGGGC CAGCAGCAGA 
GTGGCCGTCC AGAGGGGCAT AGGCCCTGGG CCACATCCTC CCGATATACA GAGACCCCCT 
CCTGGCAAAG CAGCCTTCAA ACCCAGGATT CCAGATGTCA AAGTCGTGAA TGGTGCTGGC 
CCAGAGCTGG GCACAGACCT GTCCCCAGGC TCACCCACCC TGGACATCGA CCAGTCGATC 
GAACAGCTCA ACAGGCTGAT CTTGGAGCTG GACCCCACCT TTGAGCCCAT CCCCACCCAC 
ATGAACATGT CGAGCAGCCA GGCCAACGGC CCCATGCCCC CAGACGGCAT GGCAGGCGGG 
CTATGGGCCA GTGGCCGCCT GCAGGACACA GGAGATGGCC CTGGCAGGGC CCCAGTGAGG 
CAAGGGGATG AGCCACTTGG GGGAAGATTC CGGAAGCTAA GCCTTGAGCA GTATGATAAC 
GAAGCTGGGG AGCAGCCCAC CTTCTCCAAA TGCAGCTGGG GAAAGACCAC CAGTTCAGAA 
CAGACTCCAG GCCTGGCGCC CTTTCTCTCC CCAGACAACA CCAAAGAGGC AGTGATCACC 
ACGTATCCCC CAGACCTGGA TGTGATCGAT GGCAGGATCT TCTCTCCAAC CAACAGTGGC 
AGTGAATCCA TCTCTCCCAC CCCTGCATTT CCTGTGTCTC CAGAAACGCC GTATGGGACA 
ACAAGCCCAC GTTACTCTCC GTTCAGCCCA TCCGTGCCAC AGATGGGCAG CCCCAGTGCT 
CTGTATAAAG GAGCTCATGA ACCCCGAGGC TGCCCTGAGA TTCTCAGTCA TTCTGTGGGG 
ATGTCAGAGA GCCCTGTTGG ACCCAAACCC ACGATGCTCC GGGCCGACAT GCCCACGGCG 
CCCTCCTTCC AGCGGGCATT CACGTCCTCC TGCACCATTT CTGGGAACAG CCCCAGCCAG 
AGGAGAGGGA GCCCTTCCTC AGCTGAGCAC CAGTGGGTGG AGACCAGCCC CAAGTCCACG 
CTGACCCTCC TGGGGGGCAG CCGGCCTGGC AAGGACAGCC CCGCGCGACC CCACTTCCCG 
CCTGCCGACC TCCAGACATC CTTCCACGGC CCCGAGCTGT CCCTGGCGGA GCCGCCGGAA 
GCTCTGGGTC CCCCGAGCAA CCACGCCTTC CTGAGCTTCA GCACTGCCCC CATGGCGGGA 
GGTGGGCTCC CCGCCGGGGA GGACCCCGGG GCCTTGCTGG CCAATTCCCA CGGAGCAGCC 
CAGGCCCCTG GCAACCCACT GACGGCAGCT GAGGCTGCCG ACAACAGCTT CCTGTCCCAC 
AGCTTTCTCA CGGTGGCACC TGGACACAGC GGCCACCACA GCCCGGTCCT GCAGGGCCCG 
GGACTGGCTC TGCCCGGGCA GCCACCCCTT CCTGAGAAGA AGCGGACCTC AGAGGGAGAT 
CGGTCTTTTG GCTCCGTCTC GCCATCCTCC AGTGGCTTCT CCAGTCCTCA CAGCGGGAGT 
ACCATGAGCA TCCCTTTTCC AAACATCATC CCGGACTTTT CCAAGGCTGC CGAGGGGGCA 
GCACCTTCCC CAGATAATCC AGGTGATAAG CACGTGTCTG TAAACTTTGT TCAGGACACA 
TCCAAGTTCT GGTACAAGGC GGACATCTCC AGAGAGCAAG CCATCGCCAT GCTGAAGGAC 
AAGGAGCCCG GGTCATTCAT CGTCAGGGAC AGCCACTCCT TCCGAGGTGC CTACGGCCTG 
GCCATGAAGG TGGCCACACC CCCGCCCTCT GTCCTGCAGA TGAACAAGAA AGCTGGAGAT 
TTGGCCAATG AACTTGTTCG GCATTTTTTG ATTGAGTGTA CCCCAAAAGG AGTGCGGTTG 
AAAGGGTGCT CCAATGAACC ATATTTCGGG AGCCTGACGG CTTTGGTGTG TCAGCATTCC 
ATTACGCCCC TGGCTTTGCC CTGCAAACTG CTCATTCCGG ATAGAGATCC ATTGGAGGAA 
ATAGCCGAAA ATCCTCAGCA AACAGCGGCC AACTCTGCGG CCGAGCTGCT GAAGCAAGGG 
GCAGCCTGCA ATGTGTGGTA CCTGAACTCC GTGGAGATGG AGTCCCTCAC GGGCCACCAA 
GCCATCCAGA AGGCCCTGAG CATGACCCTA GTCCAGGAGC CACCTCCCTT GTCCACAGTC 
GTGCACTTCA AGGTGTCGGC CCAGGGCATC ACCCTGACGG ACAACCAGAG GAAGCTCTTC 
TTCCGGAGAC ACTACCCCGT GAGCAGCGTG ATCTTCTGTG CGCTGGACCC ACAGGACAGA 
AAGTGGGTCA CAGACGGCCC TTGCTCGAGA GTCTTTGGAT TTGTGGCCCG GAAGCAGGGC 
AGTGCCACGG ACAACGTGTG CCACCTGTTT GCAGAGCATG ACCCCGAGCA GCCGGCCAGC 
GCCATTGTCA ACTTCGTGTC AAAGGTCATG ATCGGCTCCC CTAAGAAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNS3
Entrez Gene ID	516555
Full Name	tensin 3
General protein information	Preferred Names tensin 3 Names tensin-3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Xenopus tropicalis (tropical clawed frog)	tns3	XP_002933460.2
Pan troglodytes (chimpanzee)	TNS3	XP_519088.3
Canis lupus familiaris (dog)	TNS3	XP_005629787.1
Bos taurus (cattle)	TNS3	XP_005205666.1
Mus musculus (house mouse)	Tns3	NP_001077056.1
Gallus gallus (chicken)	TNS3	NP_001264690.1
Homo sapiens (human)	TNS3	NP_073585.8
	LOC700541	XP_002803333.1
Rattus norvegicus (Norway rat)	Tns3	NP_001163930.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNS3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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