SMG6 cDNA ORF clone, Bos taurus(cattle)

The following SMG6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226341 XM_024980134.1
Latest version!
Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa226340 XM_024980133.1
Latest version!
Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa238863 NM_001205827.2
Latest version!
Bos taurus SMG6 nonsense mediated mRNA decay factor (SMG6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226339 XM_024980132.1
Latest version!
Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa41204 NM_001205827.1
Latest version!
Bos taurus SMG6 nonsense mediated mRNA decay factor (SMG6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226341
    Clone ID Related Accession (Same CDS sequence) XM_024980134.1
    Accession Version XM_024980134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product telomerase-binding protein EST1A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCTTCA CTACTTGTGA GCTCCAGATC CAAGGGGAGC CCCTCTCCTC CCTTGGTGAG 
    TGCCCTCCCA GAAGGGAGGA GAGGACCCCA GAGGTCAGGG AGGAAGAACG GGCACGAGAA
    GGTGCAAAGC AGGTTATTGC AGCCGACTGC AAAAGGGTCA CAGTGCTGAA GTATTTTCTG
    GAAGCCCTTT GTGGACAAGA AGAGCCTCTG CTGGCATTCA AGGGTGGAAA ATATGTGTCA
    GTGGCACCCG TCCCAGACAC CATGGGAAAG GAAATGGGAA GCCAAGAGGG AAAACAACTG
    GAAGATGAGG AGGAGGACGT GGTGATTGAA GACTTTGAGG AAGACTCGGA GGCCGAAGGC
    AGCGGGGGCG AGGATGACAT CAGGGAGCTT CGGGCCAAGA AGCTGGCTCT GGCCAGGAAG
    ATAGCGGAGC AGCAGCGTCG CCAGGAGAAG ATCCAGGCTG TCCTGGAGGA CCAGAGTCAC
    GTGCGGCAGA TGGAGCTCGA AATCAGACCC TTGTTCCTCG TACCAGATAC CAACGGCTTC
    ATTGACCACC TGGCCAGCCT GGCACAGCTG CTGGAGAGCA GGAACTACAT CCTGGTGGTG
    CCCCTCATCG TGATCAATGA GCTGGATGGC CTGGCCAAGG GGCAGGAGAC AGACCACCGG
    GCCGGGGGCT ATGCCCGCGT GGTGCAGGAA AAGGCCCGAA AGTCCATTGA GTTTCTCGAG
    CGGCGATTCG AGAGTCGGGA CTCTTGCCTG CGGGCCCTGA CCAGCCGTGG CAATGAACTC
    GAATCCATCG CCTTCCGCAG CGAGGACATC ACTGGCCAGC TGGGTAACAA TGATGACCTG
    ATCCTCTCCT GCTGCCTCCA CTACTGTAAA GACAAGGCCA AGGACTTCAT GCCCACCAAC
    AAAGAGGAGC CCATCCGGCT GCTGAGGGAG GTGGTGCTGT TGACAGATGA CCGGAACCTG
    CGTGTGAAGG CGCTGACCAG GAACGTGCCT GTGCGGGACA TCCCGGCCTT CCTCACGTGG
    GCCCAGGTGG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835902.1
    CDS119..1153
    Translation

    Target ORF information:

    RefSeq Version XM_024980134.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCTTCA CTACTTGTGA GCTCCAGATC CAAGGGGAGC CCCTCTCCTC CCTTGGTGAG 
    TGCCCTCCCA GAAGGGAGGA GAGGACCCCA GAGGTCAGGG AGGAAGAACG GGCACGAGAA
    GGTGCAAAGC AGGTTATTGC AGCCGACTGC AAAAGGGTCA CAGTGCTGAA GTATTTTCTG
    GAAGCCCTTT GTGGACAAGA AGAGCCTCTG CTGGCATTCA AGGGTGGAAA ATATGTGTCA
    GTGGCACCCG TCCCAGACAC CATGGGAAAG GAAATGGGAA GCCAAGAGGG AAAACAACTG
    GAAGATGAGG AGGAGGACGT GGTGATTGAA GACTTTGAGG AAGACTCGGA GGCCGAAGGC
    AGCGGGGGCG AGGATGACAT CAGGGAGCTT CGGGCCAAGA AGCTGGCTCT GGCCAGGAAG
    ATAGCGGAGC AGCAGCGTCG CCAGGAGAAG ATCCAGGCTG TCCTGGAGGA CCAGAGTCAC
    GTGCGGCAGA TGGAGCTCGA AATCAGACCC TTGTTCCTCG TACCAGATAC CAACGGCTTC
    ATTGACCACC TGGCCAGCCT GGCACAGCTG CTGGAGAGCA GGAACTACAT CCTGGTGGTG
    CCCCTCATCG TGATCAATGA GCTGGATGGC CTGGCCAAGG GGCAGGAGAC AGACCACCGG
    GCCGGGGGCT ATGCCCGCGT GGTGCAGGAA AAGGCCCGAA AGTCCATTGA GTTTCTCGAG
    CGGCGATTCG AGAGTCGGGA CTCTTGCCTG CGGGCCCTGA CCAGCCGTGG CAATGAACTC
    GAATCCATCG CCTTCCGCAG CGAGGACATC ACTGGCCAGC TGGGTAACAA TGATGACCTG
    ATCCTCTCCT GCTGCCTCCA CTACTGTAAA GACAAGGCCA AGGACTTCAT GCCCACCAAC
    AAAGAGGAGC CCATCCGGCT GCTGAGGGAG GTGGTGCTGT TGACAGATGA CCGGAACCTG
    CGTGTGAAGG CGCTGACCAG GAACGTGCCT GTGCGGGACA TCCCGGCCTT CCTCACGTGG
    GCCCAGGTGG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226340
    Clone ID Related Accession (Same CDS sequence) XM_024980133.1
    Accession Version XM_024980133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product telomerase-binding protein EST1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG 
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTGTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCACCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835901.1
    CDS140..1675
    Translation

    Target ORF information:

    RefSeq Version XM_024980133.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG 
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTGTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCACCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238863
    Clone ID Related Accession (Same CDS sequence) NM_001205827.2
    Accession Version NM_001205827.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-29
    Organism Bos taurus(cattle)
    Product telomerase-binding protein EST1A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 25, 2018 this sequence version replaced NM_001205827.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.5036.1, SRR5721698.14513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG GGTTGGAGCG TGTCCGGATC TCCGCGTCGG AGCTGCGAGG GATCCTGGCC 
    ACTCTGGGTC CACAGGCCGG GAGCAGAGAA AACATGAAGG AGCCCAGGCA GCGAAAAGAT
    AACAGGCGCC CAGATCTGGA AATCTATAAG CCTGGTCTCT CCCGACTAAG AAACAGACCT
    AAAATCAAGG AACCCTCTGG GAGTGACGAA TCGAAGAATG AAGTTGTAAA TGACAGAGAT
    TCTTCTGCTG TTGGAAATTG TACCCAACTC ATTAAAGATG TCTGCAAAGA CAACCCACAG
    CAAAATGGTC CTGTAGACTC AGAAAATATT CAGGCACAGG AGTCCTTTCC TGGGACTGTT
    GGACAAGAGG ATCGAAGCCT AAGAATTATT AAGAGAACAA AGAAACCAGA CATGCAGATC
    TATCAGCCTG GAAGGCGTTT GCAGACTGTT AGCAAAGAAG CCACCAGCCG GGTGGAAGAG
    GAAGAAATCC TCAACCAGGT AGAGCAACTG AGGGTAGAGG AAGATGAGTG TAGGGGAAAT
    GTGAAAGAGG AGGTGGTGAA CAAGCCAGAC AGAGAGGAGG CTGAAAAGAG CCTAAGTGGT
    GACAGAGTAA GGGCTGCAAA GGGAGAAAAA GGAAAGAGGG TAGAAAGGGG GGAAGGGGTG
    AAGAAAGCAA ACGAAGATTC GGCTCAGGGG AAGCCGGGTT CTGCCAAGCG CTACTCCCGC
    TCAGACAAAC GCAGGAACCG CTACCGCACT TGCAGCACCA GCTCGGCGGG GAGCAACAAC
    AGCGCCGAGG GGGCTGGCCT GACTGACAGT GGAAGTCGCC GCCGCCGCCA GGATCGGACC
    AAGGAGAGGC CCCGACTGAA GAAGCAGGTG TCTATGTCCT CAACCGACTC CTTGGATGAG
    GACAGAATTG ATGAGCCCGA TGGACCACGG AGGAGCTCAG AGAGGAAGAA ACATTCAGAA
    AGAAGCTGGT CTGGCCGAGG GGAGGGAGAG CAGAAAAGCA ATGGTAAAGA AAATCGAGGT
    ACTCTTCGAG TCACTTTTGA TGCAGAAACC ATGAACAAAG ATTCCCCAGT GGTGAGGTCG
    GCCAGGGAAG AGGTGGATAG GTTAAAGCCA GACAAAGGCT CGAGCGGTGG GGGCAAAGGC
    TCTGAGAAGC CGGAGTCCAA AAACCTGAGA CAGGAGCTTC GGGGTCGCGG TCGGGGCATT
    CTCATTCTCC CTGCCCACAC CACCCTGTCA GTCAGTTCAG CCGGTTCTCC AGAGTCGACG
    CCTTTGGGAC CTCGGCTTTT ATTTGGAACT GGTAGCAAGG GATCTCGGAG TTGGGGCCGG
    GGAGGCACCA CTCGACGATT GTGGGACCCA AACAATCCTG AACAGAAACC CGCTCTAAAG
    AGCCAGACGC CGCAGCTACA TTTCTTGGAC ACTGATGATG AAGTCAGCCC TACCTCTTGG
    GGTGACTCAC GCCAGGCCCA AGCCTCTTAC TATAAGTTTC AGAACTCCGA CAACCCCTAT
    TATTACCCCC GGGCACCAGG CCCTGCCTCC CAGTATCCTT ACACAGGCTA TAGCCCTCTG
    CAGTACCCGG TGGGCCCTAC AAACGGTGTG TACCCAGCGC CTTACTACCC GGGCTACCCA
    GCTCCATCAG GACAGTATGT GTGTAGCCCT CTCCCTGCCA GCACCATGAG TCCTGAGGAG
    ATGGAACAGC ACATGAGGAA CATGCAGCAA CAGGAACTGC ACCGGCTCCT CCGGGTGGCT
    GACAACCAAG AACTACAACT CAGCAACCTG CTCTCCAGGG ACCGCATCAG TCCTGAGGGC
    CTGGAGAAGA TGGCTCAGCT CAGAGCTGAA CTGCTGCAGC TATATGAGCG CTGTATTCTA
    TTAGATATTG AGTTCTCTGA TAATCAGAAT GTGGATCAGA TCCTGTGGAA GAATGCTTTC
    TATCAGGTGA TTGAGAAGTT CAGGCAGCTT CTCAAGGATC CGAATGTTGA GAACCCAGAA
    CAGATTCGGA ACAGACTTTT GGAGCTCTTG GATGAGGGTA GTGACTTCTT TGATAGTTTG
    CTTCAGAAGC TGCAGGTCAC TTATAAGTTC AAACTGGAGG ACTACATGGA CGGTCTTGCC
    ATTCGCAGCA AGCCATTACG AAAGACAGTA AAATATGCCT TGATCAGTGC CCAGCGATGT
    ATGATTTGCC AAGGAGATAT TGCCAGGTAT CGGGAGCAAG CCAACGACAC GGCAAATTAT
    GGGAAGGCAC GCAGCTGGTA CCTGAAGGCC CAGCATATTG CTCCGAAGAA TGGGCGCCCT
    TATAACCAGT TGGCTCTGCT GGCTGTGTAT ACGAGGAGGA AGCTGGATGC TGTCTATTAT
    TATATGCGCA GTTTAGCTGC CAGCAACCCT ATCCTGACTG CCAAGGAGAG TCTCATGAGC
    TTGTTTGAAG AGACCAAACG GAAGGCGGAA CAGATGGAAA AGAAGCAACA CGAAGAATTG
    GAGCTGAGCC CTGACCAGTG GCGGAAAGGG AAGAAATCCA CTTTCCGACA TGTAGGAGAT
    GACACCACTC GCCTGGAGAT CTGGATTCAT CCATCCCATC CCCGGTCCTC CCAGGGCACA
    GAGTCTGGGA AGGATTCTGA GCAGGAGAAT GGGCTGGGCA GCCTAAGCCC CAGTGATCTG
    AACAAAAGGT TCATCCTCAG TTTTCTCCAT GCCCATGGGA AGCTGTTTAC CCGGATTGGG
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTGTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCACCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192756.2
    CDS1..4236
    Translation

    Target ORF information:

    RefSeq Version NM_001205827.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG6 nonsense mediated mRNA decay factor (SMG6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205827.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG GGTTGGAGCG TGTCCGGATC TCCGCGTCGG AGCTGCGAGG GATCCTGGCC 
    ACTCTGGGTC CACAGGCCGG GAGCAGAGAA AACATGAAGG AGCCCAGGCA GCGAAAAGAT
    AACAGGCGCC CAGATCTGGA AATCTATAAG CCTGGTCTCT CCCGACTAAG AAACAGACCT
    AAAATCAAGG AACCCTCTGG GAGTGACGAA TCGAAGAATG AAGTTGTAAA TGACAGAGAT
    TCTTCTGCTG TTGGAAATTG TACCCAACTC ATTAAAGATG TCTGCAAAGA CAACCCACAG
    CAAAATGGTC CTGTAGACTC AGAAAATATT CAGGCACAGG AGTCCTTTCC TGGGACTGTT
    GGACAAGAGG ATCGAAGCCT AAGAATTATT AAGAGAACAA AGAAACCAGA CATGCAGATC
    TATCAGCCTG GAAGGCGTTT GCAGACTGTT AGCAAAGAAG CCACCAGCCG GGTGGAAGAG
    GAAGAAATCC TCAACCAGGT AGAGCAACTG AGGGTAGAGG AAGATGAGTG TAGGGGAAAT
    GTGAAAGAGG AGGTGGTGAA CAAGCCAGAC AGAGAGGAGG CTGAAAAGAG CCTAAGTGGT
    GACAGAGTAA GGGCTGCAAA GGGAGAAAAA GGAAAGAGGG TAGAAAGGGG GGAAGGGGTG
    AAGAAAGCAA ACGAAGATTC GGCTCAGGGG AAGCCGGGTT CTGCCAAGCG CTACTCCCGC
    TCAGACAAAC GCAGGAACCG CTACCGCACT TGCAGCACCA GCTCGGCGGG GAGCAACAAC
    AGCGCCGAGG GGGCTGGCCT GACTGACAGT GGAAGTCGCC GCCGCCGCCA GGATCGGACC
    AAGGAGAGGC CCCGACTGAA GAAGCAGGTG TCTATGTCCT CAACCGACTC CTTGGATGAG
    GACAGAATTG ATGAGCCCGA TGGACCACGG AGGAGCTCAG AGAGGAAGAA ACATTCAGAA
    AGAAGCTGGT CTGGCCGAGG GGAGGGAGAG CAGAAAAGCA ATGGTAAAGA AAATCGAGGT
    ACTCTTCGAG TCACTTTTGA TGCAGAAACC ATGAACAAAG ATTCCCCAGT GGTGAGGTCG
    GCCAGGGAAG AGGTGGATAG GTTAAAGCCA GACAAAGGCT CGAGCGGTGG GGGCAAAGGC
    TCTGAGAAGC CGGAGTCCAA AAACCTGAGA CAGGAGCTTC GGGGTCGCGG TCGGGGCATT
    CTCATTCTCC CTGCCCACAC CACCCTGTCA GTCAGTTCAG CCGGTTCTCC AGAGTCGACG
    CCTTTGGGAC CTCGGCTTTT ATTTGGAACT GGTAGCAAGG GATCTCGGAG TTGGGGCCGG
    GGAGGCACCA CTCGACGATT GTGGGACCCA AACAATCCTG AACAGAAACC CGCTCTAAAG
    AGCCAGACGC CGCAGCTACA TTTCTTGGAC ACTGATGATG AAGTCAGCCC TACCTCTTGG
    GGTGACTCAC GCCAGGCCCA AGCCTCTTAC TATAAGTTTC AGAACTCCGA CAACCCCTAT
    TATTACCCCC GGGCACCAGG CCCTGCCTCC CAGTATCCTT ACACAGGCTA TAGCCCTCTG
    CAGTACCCGG TGGGCCCTAC AAACGGTGTG TACCCAGCGC CTTACTACCC GGGCTACCCA
    GCTCCATCAG GACAGTATGT GTGTAGCCCT CTCCCTGCCA GCACCATGAG TCCTGAGGAG
    ATGGAACAGC ACATGAGGAA CATGCAGCAA CAGGAACTGC ACCGGCTCCT CCGGGTGGCT
    GACAACCAAG AACTACAACT CAGCAACCTG CTCTCCAGGG ACCGCATCAG TCCTGAGGGC
    CTGGAGAAGA TGGCTCAGCT CAGAGCTGAA CTGCTGCAGC TATATGAGCG CTGTATTCTA
    TTAGATATTG AGTTCTCTGA TAATCAGAAT GTGGATCAGA TCCTGTGGAA GAATGCTTTC
    TATCAGGTGA TTGAGAAGTT CAGGCAGCTT CTCAAGGATC CGAATGTTGA GAACCCAGAA
    CAGATTCGGA ACAGACTTTT GGAGCTCTTG GATGAGGGTA GTGACTTCTT TGATAGTTTG
    CTTCAGAAGC TGCAGGTCAC TTATAAGTTC AAACTGGAGG ACTACATGGA CGGTCTTGCC
    ATTCGCAGCA AGCCATTACG AAAGACAGTA AAATATGCCT TGATCAGTGC CCAGCGATGT
    ATGATTTGCC AAGGAGATAT TGCCAGGTAT CGGGAGCAAG CCAACGACAC GGCAAATTAT
    GGGAAGGCAC GCAGCTGGTA CCTGAAGGCC CAGCATATTG CTCCGAAGAA TGGGCGCCCT
    TATAACCAGT TGGCTCTGCT GGCTGTGTAT ACGAGGAGGA AGCTGGATGC TGTCTATTAT
    TATATGCGCA GTTTAGCTGC CAGCAACCCT ATCCTGACTG CCAAGGAGAG TCTCATGAGC
    TTGTTTGAAG AGACCAAACG GAAGGCGGAA CAGATGGAAA AGAAGCAACA CGAAGAATTG
    GAGCTGAGCC CTGACCAGTG GCGGAAAGGG AAGAAATCCA CTTTCCGACA TGTAGGAGAT
    GACACCACTC GCCTGGAGAT CTGGATTCAT CCATCCCATC CCCGGTCCTC CCAGGGCACA
    GAGTCTGGGA AGGATTCTGA GCAGGAGAAT GGGCTGGGCA GCCTAAGCCC CAGTGATCTG
    AACAAAAGGT TCATCCTCAG TTTTCTCCAT GCCCATGGGA AGCTGTTTAC CCGGATTGGG
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTGTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCACCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226339
    Clone ID Related Accession (Same CDS sequence) XM_024980132.1
    Accession Version XM_024980132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product telomerase-binding protein EST1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGAGC CCAGGCAGCG AAAAGATAAC AGGCGCCCAG ATCTGGAAAT CTATAAGCCT 
    GGTCTCTCCC GACTAAGAAA CAGACCTAAA ATCAAGGAAC CCTCTGGGAG TGACGAATCG
    AAGAATGAAG TTGTAAATGA CAGAGATTCT TCTGCTGTTG GAAATTGTAC CCAACTCATT
    AAAGATGTCT GCAAAGACAA CCCACAGCAA AATGGTCCTG TAGACTCAGA AAATATTCAG
    GCACAGGAGT CCTTTCCTGG GACTGTTGGA CAAGAGGATC GAAGCCTAAG AATTATTAAG
    AGAACAAAGA AACCAGACAT GCAGATCTAT CAGCCTGGAA GGCGTTTGCA GACTGTTAGC
    AAAGAAGCCA CCAGCCGGGT GGAAGAGGAA GAAATCCTCA ACCAGGTAGA GCAACTGAGG
    GTAGAGGAAG ATGAGTGTAG GGGAAATGTG AAAGAGGAGG TGGTGAACAA GCCAGACAGA
    GAGGAGGCTG AAAAGAGCCT AAGTGGTGAC AGAGTAAGGG CTGCAAAGGG AGAAAAAGGA
    AAGAGGGTAG AAAGGGGGGA AGGGGTGAAG AAAGCAAACG AAGATTCGGC TCAGGGGAAG
    CCGGGTTCTG CCAAGCGCTA CTCCCGCTCA GACAAACGCA GGAACCGCTA CCGCACTTGC
    AGCACCAGCT CGGCGGGGAG CAACAACAGC GCCGAGGGGG CTGGCCTGAC TGACAGTGGA
    AGTCGCCGCC GCCGCCAGGA TCGGACCAAG GAGAGGCCCC GACTGAAGAA GCAGGTGTCT
    ATGTCCTCAA CCGACTCCTT GGATGAGGAC AGAATTGATG AGCCCGATGG ACCACGGAGG
    AGCTCAGAGA GGAAGAAACA TTCAGAAAGA AGCTGGTCTG GCCGAGGGGA GGGAGAGCAG
    AAAAGCAATG GTAAAGAAAA TCGAGGTACT CTTCGAGTCA CTTTTGATGC AGAAACCATG
    AACAAAGATT CCCCAGTGGT GAGGTCGGCC AGGGAAGAGG TGGATAGGTT AAAGCCAGAC
    AAAGGCTCGA GCGGTGGGGG CAAAGGCTCT GAGAAGCCGG AGTCCAAAAA CCTGAGACAG
    GAGCTTCGGG GTCGCGGTCG GGGCATTCTC ATTCTCCCTG CCCACACCAC CCTGTCAGTC
    AGTTCAGCCG GTTCTCCAGA GTCGACGCCT TTGGGACCTC GGCTTTTATT TGGAACTGGT
    AGCAAGGGAT CTCGGAGTTG GGGCCGGGGA GGCACCACTC GACGATTGTG GGACCCAAAC
    AATCCTGAAC AGAAACCCGC TCTAAAGAGC CAGACGCCGC AGCTACATTT CTTGGACACT
    GATGATGAAG TCAGCCCTAC CTCTTGGGGT GACTCACGCC AGGCCCAAGC CTCTTACTAT
    AAGTTTCAGA ACTCCGACAA CCCCTATTAT TACCCCCGGG CACCAGGCCC TGCCTCCCAG
    TATCCTTACA CAGGCTATAG CCCTCTGCAG TACCCGGTGG GCCCTACAAA CGGTGTGTAC
    CCAGCGCCTT ACTACCCGGG CTACCCAGCT CCATCAGGAC AGTATGTGTG TAGCCCTCTC
    CCTGCCAGCA CCATGAGTCC TGAGGAGATG GAACAGCACA TGAGGAACAT GCAGCAACAG
    GAACTGCACC GGCTCCTCCG GGTGGCTGAC AACCAAGAAC TACAACTCAG CAACCTGCTC
    TCCAGGGACC GCATCAGTCC TGAGGGCCTG GAGAAGATGG CTCAGCTCAG AGCTGAACTG
    CTGCAGCTAT ATGAGCGCTG TATTCTATTA GATATTGAGT TCTCTGATAA TCAGAATGTG
    GATCAGATCC TGTGGAAGAA TGCTTTCTAT CAGGTGATTG AGAAGTTCAG GCAGCTTCTC
    AAGGATCCGA ATGTTGAGAA CCCAGAACAG ATTCGGAACA GACTTTTGGA GCTCTTGGAT
    GAGGGTAGTG ACTTCTTTGA TAGTTTGCTT CAGAAGCTGC AGGTCACTTA TAAGTTCAAA
    CTGGAGGACT ACATGGACGG TCTTGCCATT CGCAGCAAGC CATTACGAAA GACAGTAAAA
    TATGCCTTGA TCAGTGCCCA GCGATGTATG ATTTGCCAAG GAGATATTGC CAGGTATCGG
    GAGCAAGCCA ACGACACGGC AAATTATGGG AAGGCACGCA GCTGGTACCT GAAGGCCCAG
    CATATTGCTC CGAAGAATGG GCGCCCTTAT AACCAGTTGG CTCTGCTGGC TGTGTATACG
    AGGAGGAAGC TGGATGCTGT CTATTATTAT ATGCGCAGTT TAGCTGCCAG CAACCCTATC
    CTGACTGCCA AGGAGAGTCT CATGAGCTTG TTTGAAGAGA CCAAACGGAA GGCGGAACAG
    ATGGAAAAGA AGCAACACGA AGAATTGGAG CTGAGCCCTG ACCAGTGGCG GAAAGGGAAG
    AAATCCACTT TCCGACATGT AGGAGATGAC ACCACTCGCC TGGAGATCTG GATTCATCCA
    TCCCATCCCC GGTCCTCCCA GGGCACAGAG TCTGGGAAGG ATTCTGAGCA GGAGAATGGG
    CTGGGCAGCC TAAGCCCCAG TGATCTGAAC AAAAGGTTCA TCCTCAGTTT TCTCCATGCC
    CATGGGAAGC TGTTTACCCG GATTGGGATG GAGACGTTCC CTGCAGTGGC TGAGAAGGTC
    CTCAAGGAGT TCCAGGTGTT GCTGCAGCAC AGCCCCTCTC CTATTGGAAG TACCCGCATG
    CTACAGCTTA TGACCATCAA CATGTTTGCT GTACACAACT CCCAGTTGAA AGACTGCTTC
    TCGGAGGAGT GTCGCTCTGT GATCCAGGAG CAGGCTGCAG CCCTGGGCCT GGCCATGTTT
    TCTCTCCTGG TGCGACGCTG CACCTACTTA CTGAAGGAGT CGGCCAAAGC TCAGCTGTCC
    TCTCCTGAGG GAGAGGAGGA TCAAGATGAC ATCAAGGTGT CCTCCTTTGT CCCGGACCTG
    AAGGAACTGC TCCCCAGTGT GAAGGTCTGG TCAGACTGGA TGCTCGGCTA CCCAGACACC
    TGGAACCCTC CACCTACCTC CCTGGAGCTG CCCTCACAGG TTGCTGTGGA TGTGTGGTCG
    ACGCTGGCTG ACTTCTGTAA CATACTGACT GCAGTGAATC AGTCTGAGGT GCCACTGTAC
    AAGGACCCGG ACGATGACCT CACCCTCCTC ATCTTGGAAG AGGATCGGCT TCTCTCGGGC
    TTTGTCCCCT TGCTGGCTGC CCCTCAGGAC CCCTGCTACG TGGAGAAAAC CTCGGATAAG
    GTTATTGCAG CCGACTGCAA AAGGGTCACA GTGCTGAAGT ATTTTCTGGA AGCCCTTTGT
    GGACAAGAAG AGCCTCTGCT GGCATTCAAG GGTGGAAAAT ATGTGTCAGT GGCACCCGTC
    CCAGACACCA TGGGAAAGGA AATGGGAAGC CAAGAGGGAA AACAACTGGA AGATGAGGAG
    GAGGACGTGG TGATTGAAGA CTTTGAGGAA GACTCGGAGG CCGAAGGCAG CGGGGGCGAG
    GATGACATCA GGGAGCTTCG GGCCAAGAAG CTGGCTCTGG CCAGGAAGAT AGCGGAGCAG
    CAGCGTCGCC AGGAGAAGAT CCAGGCTGTC CTGGAGGACC AGAGTCACGT GCGGCAGATG
    GAGCTCGAAA TCAGACCCTT GTTCCTCGTA CCAGATACCA ACGGCTTCAT TGACCACCTG
    GCCAGCCTGG CACAGCTGCT GGAGAGCAGG AACTACATCC TGGTGGTGCC CCTCATCGTG
    ATCAATGAGC TGGATGGCCT GGCCAAGGGG CAGGAGACAG ACCACCGGGC CGGGGGCTAT
    GCCCGCGTGG TGCAGGAAAA GGCCCGAAAG TCCATTGAGT TTCTCGAGCG GCGATTCGAG
    AGTCGGGACT CTTGCCTGCG GGCCCTGACC AGCCGTGGCA ATGAACTCGA ATCCATCGCC
    TTCCGCAGCG AGGACATCAC TGGCCAGCTG GGTAACAATG ATGACCTGAT CCTCTCCTGC
    TGCCTCCACT ACTGTAAAGA CAAGGCCAAG GACTTCATGC CCACCAACAA AGAGGAGCCC
    ATCCGGCTGC TGAGGGAGGT GGTGCTGTTG ACAGATGACC GGAACCTGCG TGTGAAGGCG
    CTGACCAGGA ACGTGCCTGT GCGGGACATC CCGGCCTTCC TCACGTGGGC CCAGGTGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835900.1
    CDS154..4296
    Translation

    Target ORF information:

    RefSeq Version XM_024980132.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG6, nonsense mediated mRNA decay factor (SMG6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGAGC CCAGGCAGCG AAAAGATAAC AGGCGCCCAG ATCTGGAAAT CTATAAGCCT 
    GGTCTCTCCC GACTAAGAAA CAGACCTAAA ATCAAGGAAC CCTCTGGGAG TGACGAATCG
    AAGAATGAAG TTGTAAATGA CAGAGATTCT TCTGCTGTTG GAAATTGTAC CCAACTCATT
    AAAGATGTCT GCAAAGACAA CCCACAGCAA AATGGTCCTG TAGACTCAGA AAATATTCAG
    GCACAGGAGT CCTTTCCTGG GACTGTTGGA CAAGAGGATC GAAGCCTAAG AATTATTAAG
    AGAACAAAGA AACCAGACAT GCAGATCTAT CAGCCTGGAA GGCGTTTGCA GACTGTTAGC
    AAAGAAGCCA CCAGCCGGGT GGAAGAGGAA GAAATCCTCA ACCAGGTAGA GCAACTGAGG
    GTAGAGGAAG ATGAGTGTAG GGGAAATGTG AAAGAGGAGG TGGTGAACAA GCCAGACAGA
    GAGGAGGCTG AAAAGAGCCT AAGTGGTGAC AGAGTAAGGG CTGCAAAGGG AGAAAAAGGA
    AAGAGGGTAG AAAGGGGGGA AGGGGTGAAG AAAGCAAACG AAGATTCGGC TCAGGGGAAG
    CCGGGTTCTG CCAAGCGCTA CTCCCGCTCA GACAAACGCA GGAACCGCTA CCGCACTTGC
    AGCACCAGCT CGGCGGGGAG CAACAACAGC GCCGAGGGGG CTGGCCTGAC TGACAGTGGA
    AGTCGCCGCC GCCGCCAGGA TCGGACCAAG GAGAGGCCCC GACTGAAGAA GCAGGTGTCT
    ATGTCCTCAA CCGACTCCTT GGATGAGGAC AGAATTGATG AGCCCGATGG ACCACGGAGG
    AGCTCAGAGA GGAAGAAACA TTCAGAAAGA AGCTGGTCTG GCCGAGGGGA GGGAGAGCAG
    AAAAGCAATG GTAAAGAAAA TCGAGGTACT CTTCGAGTCA CTTTTGATGC AGAAACCATG
    AACAAAGATT CCCCAGTGGT GAGGTCGGCC AGGGAAGAGG TGGATAGGTT AAAGCCAGAC
    AAAGGCTCGA GCGGTGGGGG CAAAGGCTCT GAGAAGCCGG AGTCCAAAAA CCTGAGACAG
    GAGCTTCGGG GTCGCGGTCG GGGCATTCTC ATTCTCCCTG CCCACACCAC CCTGTCAGTC
    AGTTCAGCCG GTTCTCCAGA GTCGACGCCT TTGGGACCTC GGCTTTTATT TGGAACTGGT
    AGCAAGGGAT CTCGGAGTTG GGGCCGGGGA GGCACCACTC GACGATTGTG GGACCCAAAC
    AATCCTGAAC AGAAACCCGC TCTAAAGAGC CAGACGCCGC AGCTACATTT CTTGGACACT
    GATGATGAAG TCAGCCCTAC CTCTTGGGGT GACTCACGCC AGGCCCAAGC CTCTTACTAT
    AAGTTTCAGA ACTCCGACAA CCCCTATTAT TACCCCCGGG CACCAGGCCC TGCCTCCCAG
    TATCCTTACA CAGGCTATAG CCCTCTGCAG TACCCGGTGG GCCCTACAAA CGGTGTGTAC
    CCAGCGCCTT ACTACCCGGG CTACCCAGCT CCATCAGGAC AGTATGTGTG TAGCCCTCTC
    CCTGCCAGCA CCATGAGTCC TGAGGAGATG GAACAGCACA TGAGGAACAT GCAGCAACAG
    GAACTGCACC GGCTCCTCCG GGTGGCTGAC AACCAAGAAC TACAACTCAG CAACCTGCTC
    TCCAGGGACC GCATCAGTCC TGAGGGCCTG GAGAAGATGG CTCAGCTCAG AGCTGAACTG
    CTGCAGCTAT ATGAGCGCTG TATTCTATTA GATATTGAGT TCTCTGATAA TCAGAATGTG
    GATCAGATCC TGTGGAAGAA TGCTTTCTAT CAGGTGATTG AGAAGTTCAG GCAGCTTCTC
    AAGGATCCGA ATGTTGAGAA CCCAGAACAG ATTCGGAACA GACTTTTGGA GCTCTTGGAT
    GAGGGTAGTG ACTTCTTTGA TAGTTTGCTT CAGAAGCTGC AGGTCACTTA TAAGTTCAAA
    CTGGAGGACT ACATGGACGG TCTTGCCATT CGCAGCAAGC CATTACGAAA GACAGTAAAA
    TATGCCTTGA TCAGTGCCCA GCGATGTATG ATTTGCCAAG GAGATATTGC CAGGTATCGG
    GAGCAAGCCA ACGACACGGC AAATTATGGG AAGGCACGCA GCTGGTACCT GAAGGCCCAG
    CATATTGCTC CGAAGAATGG GCGCCCTTAT AACCAGTTGG CTCTGCTGGC TGTGTATACG
    AGGAGGAAGC TGGATGCTGT CTATTATTAT ATGCGCAGTT TAGCTGCCAG CAACCCTATC
    CTGACTGCCA AGGAGAGTCT CATGAGCTTG TTTGAAGAGA CCAAACGGAA GGCGGAACAG
    ATGGAAAAGA AGCAACACGA AGAATTGGAG CTGAGCCCTG ACCAGTGGCG GAAAGGGAAG
    AAATCCACTT TCCGACATGT AGGAGATGAC ACCACTCGCC TGGAGATCTG GATTCATCCA
    TCCCATCCCC GGTCCTCCCA GGGCACAGAG TCTGGGAAGG ATTCTGAGCA GGAGAATGGG
    CTGGGCAGCC TAAGCCCCAG TGATCTGAAC AAAAGGTTCA TCCTCAGTTT TCTCCATGCC
    CATGGGAAGC TGTTTACCCG GATTGGGATG GAGACGTTCC CTGCAGTGGC TGAGAAGGTC
    CTCAAGGAGT TCCAGGTGTT GCTGCAGCAC AGCCCCTCTC CTATTGGAAG TACCCGCATG
    CTACAGCTTA TGACCATCAA CATGTTTGCT GTACACAACT CCCAGTTGAA AGACTGCTTC
    TCGGAGGAGT GTCGCTCTGT GATCCAGGAG CAGGCTGCAG CCCTGGGCCT GGCCATGTTT
    TCTCTCCTGG TGCGACGCTG CACCTACTTA CTGAAGGAGT CGGCCAAAGC TCAGCTGTCC
    TCTCCTGAGG GAGAGGAGGA TCAAGATGAC ATCAAGGTGT CCTCCTTTGT CCCGGACCTG
    AAGGAACTGC TCCCCAGTGT GAAGGTCTGG TCAGACTGGA TGCTCGGCTA CCCAGACACC
    TGGAACCCTC CACCTACCTC CCTGGAGCTG CCCTCACAGG TTGCTGTGGA TGTGTGGTCG
    ACGCTGGCTG ACTTCTGTAA CATACTGACT GCAGTGAATC AGTCTGAGGT GCCACTGTAC
    AAGGACCCGG ACGATGACCT CACCCTCCTC ATCTTGGAAG AGGATCGGCT TCTCTCGGGC
    TTTGTCCCCT TGCTGGCTGC CCCTCAGGAC CCCTGCTACG TGGAGAAAAC CTCGGATAAG
    GTTATTGCAG CCGACTGCAA AAGGGTCACA GTGCTGAAGT ATTTTCTGGA AGCCCTTTGT
    GGACAAGAAG AGCCTCTGCT GGCATTCAAG GGTGGAAAAT ATGTGTCAGT GGCACCCGTC
    CCAGACACCA TGGGAAAGGA AATGGGAAGC CAAGAGGGAA AACAACTGGA AGATGAGGAG
    GAGGACGTGG TGATTGAAGA CTTTGAGGAA GACTCGGAGG CCGAAGGCAG CGGGGGCGAG
    GATGACATCA GGGAGCTTCG GGCCAAGAAG CTGGCTCTGG CCAGGAAGAT AGCGGAGCAG
    CAGCGTCGCC AGGAGAAGAT CCAGGCTGTC CTGGAGGACC AGAGTCACGT GCGGCAGATG
    GAGCTCGAAA TCAGACCCTT GTTCCTCGTA CCAGATACCA ACGGCTTCAT TGACCACCTG
    GCCAGCCTGG CACAGCTGCT GGAGAGCAGG AACTACATCC TGGTGGTGCC CCTCATCGTG
    ATCAATGAGC TGGATGGCCT GGCCAAGGGG CAGGAGACAG ACCACCGGGC CGGGGGCTAT
    GCCCGCGTGG TGCAGGAAAA GGCCCGAAAG TCCATTGAGT TTCTCGAGCG GCGATTCGAG
    AGTCGGGACT CTTGCCTGCG GGCCCTGACC AGCCGTGGCA ATGAACTCGA ATCCATCGCC
    TTCCGCAGCG AGGACATCAC TGGCCAGCTG GGTAACAATG ATGACCTGAT CCTCTCCTGC
    TGCCTCCACT ACTGTAAAGA CAAGGCCAAG GACTTCATGC CCACCAACAA AGAGGAGCCC
    ATCCGGCTGC TGAGGGAGGT GGTGCTGTTG ACAGATGACC GGAACCTGCG TGTGAAGGCG
    CTGACCAGGA ACGTGCCTGT GCGGGACATC CCGGCCTTCC TCACGTGGGC CCAGGTGGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41204
    Clone ID Related Accession (Same CDS sequence) NM_001205827.1
    Accession Version NM_001205827.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product telomerase-binding protein EST1A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03014223.1, AAFC03014221.1, AAFC03014222.1 and AAFC03026633.1. On Apr 20, 2011 this sequence version replaced XM_594564.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG GGTTGGAGCG TGTCCGGATC TCCGCGTCGG AGCTGCGAGG GATCCTGGCC 
    ACTCTGGGTC CACAGGCCGG GAGCAGAGAA AACATGAAGG AGCCCAGGCA GCGAAAAGAT
    AACAGGCGCC CAGATCTGGA AATCTATAAG CCTGGTCTCT CCCGACTAAG AAACAGACCT
    AAAATCAAGG AACCCTCTGG GAGTGACGAA TCGAAGAATG AAGTTGTAAA TGACAGAGAT
    TCTTCTGCTG TTGGAAATTG TACCCAACTC ATTAAAGATG TCTGCAAAGA CAACCCACAG
    CAAAATGGTC CTGTAGACTC AGAAAATATT CAGGCACAGG AGTCCTTTCC TGGGACTGTT
    GGACAAGAGG ATCGAAGCCT AAGAATTATT AAGAGAACAA AGAAACCAGA CATGCAGATC
    TATCAGCCTG GAAGGCGTTT GCAGACTGTT AGCAAAGAAG CCACCAGCCG GGTGGAAGAG
    GAAGAAATCC TCAACCAGGT AGAGCAACTG AGGGTAGAGG AAGATGAGTG TAGGGGAAAT
    GTGAAAGAGG AGGTGGTGAA CAAGCCAGAC AGAGAGGAGG CTGAAAAGAG CCTAAGTGGT
    GACAGAGTAA GGGCTGCAAA GGGAGAAAAA GGAAAGAGGG TAGAAAGGGG GGAAGGGGTG
    AAGAAAGCAA ACGAAGATTC GGCTCAGGGG AAGCCGGGTT CTGCCAAGCG CTACTCCCGC
    TCAGACAAAC GCAGGAACCG CTACCGCACT TGCAGCACCA GCTCGGCGGG GAGCAACAAC
    AGCGCCGAGG GGGCTGGCCT GACTGACAGT GGAAGTCGCC GCCGCCGCCA GGATCGGACC
    AAGGAGAGGC CCCGACTGAA GAAGCAGGTG TCTATGTCCT CAACCGACTC CTTGGATGAG
    GACAGAATTG ATGAGCCCGA TGGACCACGG AGGAGCTCAG AGAGGAAGAA ACATTCAGAA
    AGAAGCTGGT CTGGCCGAGG GGAGGGAGAG CAGAAAAGCA ATGGTAAAGA AAATCGAGGT
    ACTCTTCGAG TCACTTTTGA TGCAGAAACC ATGAACAAAG ATTCCCCAGT GGTGAGGTCG
    GCCAGGGAAG AGGTGGATAG GTTAAAGCCA GACAAAGGCT CGAGCGGTGG GGGCAAAGGC
    TCTGAGAAGC CGGAGTCCAA AAACCTGAGA CAGGAGCTTC GGGGTCGCGG TCGGGGCATT
    CTCATTCTCC CTGCCCACAC CACCCTGTCA GTCAGTTCAG CCGGTTCTCC AGAGTCGACG
    CCTTTGGGAC CTCGGCTTTT ATTTGGAACT GGTAGCAAGG GATCTCGGAG TTGGGGCCGG
    GGAGGCACCA CTCGACGATT GTGGGACCCA AACAATCCTG AACAGAAACC CGCTCTAAAG
    AGCCAGACGC CGCAGCTACA TTTCTTGGAC ACTGATGATG AAGTCAGCCC TACCTCTTGG
    GGTGACTCAC GCCAGGCCCA AGCCTCTTAC TATAAGTTTC AGAACTCCGA CAACCCCTAT
    TATTACCCCC GGGCACCAGG CCCTGCCTCC CAGTATCCTT ACACAGGCTA TAGCCCTCTG
    CAGTACCCGG TGGGCCCTAC AAACGGTGTG TACCCAGCGC CTTACTACCC GGGCTACCCA
    GCTCCATCAG GACAGTATGT GTGTAGCCCT CTCCCTGCCA GCACCATGAG TCCTGAGGAG
    ATGGAACAGC ACATGAGGAA CATGCAGCAA CAGGAACTGC ACCGGCTCCT CCGGGTGGCT
    GACAACCAAG AACTACAACT CAGCAACCTG CTCTCCAGGG ACCGCATCAG TCCTGAGGGC
    CTGGAGAAGA TGGCTCAGCT CAGAGCTGAA CTGCTGCAGC TATATGAGCG CTGTATTCTA
    TTAGATATTG AGTTCTCTGA TAATCAGAAT GTGGATCAGA TCCTGTGGAA GAATGCTTTC
    TATCAGGTGA TTGAGAAGTT CAGGCAGCTT CTCAAGGATC CGAATGTTGA GAACCCAGAA
    CAGATTCGGA ACAGACTTTT GGAGCTCTTG GATGAGGGTA GTGACTTCTT TGATAGTTTG
    CTTCAGAAGC TGCAGGTCAC TTATAAGTTC AAACTGGAGG ACTACATGGA CGGTCTTGCC
    ATTCGCAGCA AGCCATTACG AAAGACAGTA AAATATGCCT TGATCAGTGC CCAGCGATGT
    ATGATTTGCC AAGGAGATAT TGCCAGGTAT CGGGAGCAAG CCAACGACAC GGCAAATTAT
    GGGAAGGCAC GCAGCTGGTA CCTGAAGGCC CAGCATATTG CTCCGAAGAA TGGGCGCCCT
    TATAACCAGT TGGCTCTGCT GGCTGTGTAT ACGAGGAGGA AGCTGGATGC TGTCTATTAT
    TATATGCGCA GTTTAGCTGC CAGCAACCCT ATCCTGACTG CCAAGGAGAG TCTCATGAGC
    TTGTTTGAAG AGACCAAACG GAAGGCGGAA CAGATGGAAA AGAAGCAACA CGAAGAATTG
    GAGCTGAGCC CTGACCAGTG GCGGAAAGGG AAGAAATCCA CTTTCCGACA TGTAGGAGAT
    GACACCACTC GCCTGGAGAT CTGGATTCAT CCATCCCATC CCCGGTCCTC CCAGGGCACA
    GAGTCTGGGA AGGATTCTGA GCAGGAGAAT GGGCTGGGCA GCCTAAGCCC CAGTGATCTG
    AACAAAAGGT TCATCCTCAG TTTTCTCCAT GCCCATGGGA AGCTGTTTAC CCGGATTGGG
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTTTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCGCCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192756.1
    CDS1..4236
    Translation

    Target ORF information:

    RefSeq Version NM_001205827.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG6 nonsense mediated mRNA decay factor (SMG6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG GGTTGGAGCG TGTCCGGATC TCCGCGTCGG AGCTGCGAGG GATCCTGGCC 
    ACTCTGGGTC CACAGGCCGG GAGCAGAGAA AACATGAAGG AGCCCAGGCA GCGAAAAGAT
    AACAGGCGCC CAGATCTGGA AATCTATAAG CCTGGTCTCT CCCGACTAAG AAACAGACCT
    AAAATCAAGG AACCCTCTGG GAGTGACGAA TCGAAGAATG AAGTTGTAAA TGACAGAGAT
    TCTTCTGCTG TTGGAAATTG TACCCAACTC ATTAAAGATG TCTGCAAAGA CAACCCACAG
    CAAAATGGTC CTGTAGACTC AGAAAATATT CAGGCACAGG AGTCCTTTCC TGGGACTGTT
    GGACAAGAGG ATCGAAGCCT AAGAATTATT AAGAGAACAA AGAAACCAGA CATGCAGATC
    TATCAGCCTG GAAGGCGTTT GCAGACTGTT AGCAAAGAAG CCACCAGCCG GGTGGAAGAG
    GAAGAAATCC TCAACCAGGT AGAGCAACTG AGGGTAGAGG AAGATGAGTG TAGGGGAAAT
    GTGAAAGAGG AGGTGGTGAA CAAGCCAGAC AGAGAGGAGG CTGAAAAGAG CCTAAGTGGT
    GACAGAGTAA GGGCTGCAAA GGGAGAAAAA GGAAAGAGGG TAGAAAGGGG GGAAGGGGTG
    AAGAAAGCAA ACGAAGATTC GGCTCAGGGG AAGCCGGGTT CTGCCAAGCG CTACTCCCGC
    TCAGACAAAC GCAGGAACCG CTACCGCACT TGCAGCACCA GCTCGGCGGG GAGCAACAAC
    AGCGCCGAGG GGGCTGGCCT GACTGACAGT GGAAGTCGCC GCCGCCGCCA GGATCGGACC
    AAGGAGAGGC CCCGACTGAA GAAGCAGGTG TCTATGTCCT CAACCGACTC CTTGGATGAG
    GACAGAATTG ATGAGCCCGA TGGACCACGG AGGAGCTCAG AGAGGAAGAA ACATTCAGAA
    AGAAGCTGGT CTGGCCGAGG GGAGGGAGAG CAGAAAAGCA ATGGTAAAGA AAATCGAGGT
    ACTCTTCGAG TCACTTTTGA TGCAGAAACC ATGAACAAAG ATTCCCCAGT GGTGAGGTCG
    GCCAGGGAAG AGGTGGATAG GTTAAAGCCA GACAAAGGCT CGAGCGGTGG GGGCAAAGGC
    TCTGAGAAGC CGGAGTCCAA AAACCTGAGA CAGGAGCTTC GGGGTCGCGG TCGGGGCATT
    CTCATTCTCC CTGCCCACAC CACCCTGTCA GTCAGTTCAG CCGGTTCTCC AGAGTCGACG
    CCTTTGGGAC CTCGGCTTTT ATTTGGAACT GGTAGCAAGG GATCTCGGAG TTGGGGCCGG
    GGAGGCACCA CTCGACGATT GTGGGACCCA AACAATCCTG AACAGAAACC CGCTCTAAAG
    AGCCAGACGC CGCAGCTACA TTTCTTGGAC ACTGATGATG AAGTCAGCCC TACCTCTTGG
    GGTGACTCAC GCCAGGCCCA AGCCTCTTAC TATAAGTTTC AGAACTCCGA CAACCCCTAT
    TATTACCCCC GGGCACCAGG CCCTGCCTCC CAGTATCCTT ACACAGGCTA TAGCCCTCTG
    CAGTACCCGG TGGGCCCTAC AAACGGTGTG TACCCAGCGC CTTACTACCC GGGCTACCCA
    GCTCCATCAG GACAGTATGT GTGTAGCCCT CTCCCTGCCA GCACCATGAG TCCTGAGGAG
    ATGGAACAGC ACATGAGGAA CATGCAGCAA CAGGAACTGC ACCGGCTCCT CCGGGTGGCT
    GACAACCAAG AACTACAACT CAGCAACCTG CTCTCCAGGG ACCGCATCAG TCCTGAGGGC
    CTGGAGAAGA TGGCTCAGCT CAGAGCTGAA CTGCTGCAGC TATATGAGCG CTGTATTCTA
    TTAGATATTG AGTTCTCTGA TAATCAGAAT GTGGATCAGA TCCTGTGGAA GAATGCTTTC
    TATCAGGTGA TTGAGAAGTT CAGGCAGCTT CTCAAGGATC CGAATGTTGA GAACCCAGAA
    CAGATTCGGA ACAGACTTTT GGAGCTCTTG GATGAGGGTA GTGACTTCTT TGATAGTTTG
    CTTCAGAAGC TGCAGGTCAC TTATAAGTTC AAACTGGAGG ACTACATGGA CGGTCTTGCC
    ATTCGCAGCA AGCCATTACG AAAGACAGTA AAATATGCCT TGATCAGTGC CCAGCGATGT
    ATGATTTGCC AAGGAGATAT TGCCAGGTAT CGGGAGCAAG CCAACGACAC GGCAAATTAT
    GGGAAGGCAC GCAGCTGGTA CCTGAAGGCC CAGCATATTG CTCCGAAGAA TGGGCGCCCT
    TATAACCAGT TGGCTCTGCT GGCTGTGTAT ACGAGGAGGA AGCTGGATGC TGTCTATTAT
    TATATGCGCA GTTTAGCTGC CAGCAACCCT ATCCTGACTG CCAAGGAGAG TCTCATGAGC
    TTGTTTGAAG AGACCAAACG GAAGGCGGAA CAGATGGAAA AGAAGCAACA CGAAGAATTG
    GAGCTGAGCC CTGACCAGTG GCGGAAAGGG AAGAAATCCA CTTTCCGACA TGTAGGAGAT
    GACACCACTC GCCTGGAGAT CTGGATTCAT CCATCCCATC CCCGGTCCTC CCAGGGCACA
    GAGTCTGGGA AGGATTCTGA GCAGGAGAAT GGGCTGGGCA GCCTAAGCCC CAGTGATCTG
    AACAAAAGGT TCATCCTCAG TTTTCTCCAT GCCCATGGGA AGCTGTTTAC CCGGATTGGG
    ATGGAGACGT TCCCTGCAGT GGCTGAGAAG GTCCTCAAGG AGTTCCAGGT GTTGCTGCAG
    CACAGCCCCT CTCCTATTGG AAGTACCCGC ATGCTACAGC TTATGACCAT CAACATGTTT
    GCTTTACACA ACTCCCAGTT GAAAGACTGC TTCTCGGAGG AGTGTCGCTC TGTGATCCAG
    GAGCAGGCTG CAGCCCTGGG CCTGGCCATG TTTTCTCTCC TGGTGCGACG CTGCACCTAC
    TTACTGAAGG AGTCGGCCAA AGCTCAGCTG TCCTCTCCTG AGGGAGAGGA GGATCAAGAT
    GACATCAAGG TGTCCTCCTT TGTCCCGGAC CTGAAGGAAC TGCTCCCCAG TGTGAAGGTC
    TGGTCAGACT GGATGCTCGG CTACCCAGAC ACCTGGAACC CTCCGCCTAC CTCCCTGGAG
    CTGCCCTCAC AGGTTGCTGT GGATGTGTGG TCGACGCTGG CTGACTTCTG TAACATACTG
    ACTGCAGTGA ATCAGTCTGA GGTGCCACTG TACAAGGACC CGGACGATGA CCTCACCCTC
    CTCATCTTGG AAGAGGATCG GCTTCTCTCG GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG
    GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCCGACTG CAAAAGGGTC
    ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC
    AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA
    AGCCAAGAGG GAAAACAACT GGAAGATGAG GAGGAGGACG TGGTGATTGA AGACTTTGAG
    GAAGACTCGG AGGCCGAAGG CAGCGGGGGC GAGGATGACA TCAGGGAGCT TCGGGCCAAG
    AAGCTGGCTC TGGCCAGGAA GATAGCGGAG CAGCAGCGTC GCCAGGAGAA GATCCAGGCT
    GTCCTGGAGG ACCAGAGTCA CGTGCGGCAG ATGGAGCTCG AAATCAGACC CTTGTTCCTC
    GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGCC TGGCACAGCT GCTGGAGAGC
    AGGAACTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGATGG CCTGGCCAAG
    GGGCAGGAGA CAGACCACCG GGCCGGGGGC TATGCCCGCG TGGTGCAGGA AAAGGCCCGA
    AAGTCCATTG AGTTTCTCGA GCGGCGATTC GAGAGTCGGG ACTCTTGCCT GCGGGCCCTG
    ACCAGCCGTG GCAATGAACT CGAATCCATC GCCTTCCGCA GCGAGGACAT CACTGGCCAG
    CTGGGTAACA ATGATGACCT GATCCTCTCC TGCTGCCTCC ACTACTGTAA AGACAAGGCC
    AAGGACTTCA TGCCCACCAA CAAAGAGGAG CCCATCCGGC TGCTGAGGGA GGTGGTGCTG
    TTGACAGATG ACCGGAACCT GCGTGTGAAG GCGCTGACCA GGAACGTGCC TGTGCGGGAC
    ATCCCGGCCT TCCTCACGTG GGCCCAGGTG GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.