ARHGEF5 cDNA ORF clone, Bos taurus(cattle)

The following ARHGEF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa233662 XM_024990664.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa18861 NM_001110075.2
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18861 XM_005205839.3
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa18861 XM_005205839.2 Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa233662
    Clone ID Related Accession (Same CDS sequence) XM_024990664.1
    Accession Version XM_024990664.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGACGGCCC TTCTACACCC ATCTTCCCCG ACCACCCCGT GGACTCCAGA GGTCCAGCGA 
    CCCACCTCTG AACCCGGGCT CTCAGAAGAG TCTGAAGCCC CTGCCAGAGG GTCTTGGAGA
    AGAACAGCCC CCCAGGAAGG AAGCAATGGC CTGAGGAGGT CAGAGGTAGG CCCGGTGAGG
    CAGCCGGAGA AATCGGGCCA CATCCTCCTG GAGAAGGCAT CGAGCTGGCC CCATCGACGG
    GACCCGAGAA GGCCAGTGGA GGGCGGCAGT GAGGTGGCGG CGGTCCCCGG TGAAGGGTCG
    AGTAAGCACA AGGACTGGCA CCGGCAGGGC CTGCGAAGAC CCTCCATCCT CCCCGAGAGC
    CCTGTAGACA CAAGAGGTCC CGCCATGGAA GGATCCCCTG GCCTGTCGGA CGCCATTGTT
    TTCCGGGAGA AAAAGCCAAA GGAGGTGATG GGAAACTTTT CAAGACGGTG CTCCAAGCTT
    ATCAACTCCT CTCAGCTGCT TTACCAGGAG TACAGTGATG TGTTTCTGAA CAAGGAGATT
    CAGAGCCAGC AGCGGTTGGA CAGCCTGACG GAATCACCGG GGCCCATCTC GCCCCGGCAG
    CCCCGAAAGG CCCTTGTCTC CTCGGAGTCC TACCTGCGTC GCCTCTCCAT GGCCTCCAGC
    GGCTCCCTGT GGCAGGAGAT CCCTGTGGTG CGCAATAGCA CCGTGCTGCT CTCCATGACC
    CATGAAGACC AGAAGCTCCA GGAGGCCAAA TTTGAGCTGA TTGTGTCAGA GGCCTCCTAC
    CTGCGCAGTT TGCATATAGC TGTGGATCAC TTCCAACATT CAGAGCAGCT CCGGGCCACC
    CTTTCCAACC AGGATCACCA ATGGCTCTTC TCTCGTTTGC AGGATGTGCG TGACGTCAGC
    ACCATGTTCC TTTCTGACCT GGAAGAGAAC TTTGAGAACA ACATCTTCAC CTTCCAAGTG
    TGTGATGTGG TCCTGAACCA TGCCCCCAAC TTCCGTCGGG TCTACCTGCC TTATGTCACC
    AACCAGACCT ACCAGGAACG CACCTTCCAA AGCCTGCTGA ACAGCAACAG CAGTTTCCGA
    GAGGTCCTGG AGAAGCTGGA GAGTGCCCCC ATCTGCCAGC GCCTTTCCCT CAAGTCCTTC
    CTGATTCTGC CTTTCCAGCG CATCACCCGT CTCAAGCTCC TGCTCCAGAA CATTCTGAAG
    AGAACACAGC CCGGCTCCGC AGAGGAAGCA GAGGCCACGA AAGCACACCA TGCCCTGGAG
    GAGCTGATCC GAGACTGCAA TAACAATGTC CAGAGGATGC GACGGACGGA GGAGCTGATC
    TACCTGAGCC AGAAGATTGA GTTTGAGTGC AAAATATTCC CGCTCATTTC ACAATCGCGC
    TGGCTGGTGA AGAGTGGGGA GCTGACGGCC CTGGAGCTCA GCGTCTCCCA GGCGCTGCGG
    AGGAAGCTGA CCACCCGCCC GGTCCACCTG CACCTCTTCA ACGACTGTCT GTTGCTGTCT
    CGGCCCCGAG AGGGTAGCCG ATTCCTGGTA TTCGACCATG CTCCCTTCTC TGCCGTCCGT
    GGGGAAAAGT GTGAAATGAA GCTGCATGGA GCTCACAAAA ACCTCTTCCG ACTCTTCCTG
    CGGCGCAACG CCCAGGGCTC CCAGGCCGAG TTCCTCCTCC GCACAGAGAC CCAAAGTGAA
    AAGCTTCGCT GGATCTCAGC CTTGGCTATG CCGAGGGAGG AGTTGGACCT TCTGGAGTGT
    TACGACTCCC CACAAGTGCA GTGCCTTAGA GCCTACAAAC CCCGAGAGAA TGACGAGTTG
    GCGCTCGAGA AGGCAGACGT GGTGATGGTG ACCCAGCAGA GCAGTGATGG CTGGCTGGAA
    GGCGTGAGAC TGTCCGATGG GGAGCAAGGC TGGTTCCCTC TACAACAAGT AGAGTTCATC
    TCCAACCCAG AGGTCCGCGC ACGGAACCTG AAGGAAGCCC ACCGAGTCAA GACTGCCAAG
    CTACAGCTGG TGGAGCAGCA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846432.1
    CDS168..2174
    Translation

    Target ORF information:

    RefSeq Version XM_024990664.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990664.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGACGGCCC TTCTACACCC ATCTTCCCCG ACCACCCCGT GGACTCCAGA GGTCCAGCGA 
    CCCACCTCTG AACCCGGGCT CTCAGAAGAG TCTGAAGCCC CTGCCAGAGG GTCTTGGAGA
    AGAACAGCCC CCCAGGAAGG AAGCAATGGC CTGAGGAGGT CAGAGGTAGG CCCGGTGAGG
    CAGCCGGAGA AATCGGGCCA CATCCTCCTG GAGAAGGCAT CGAGCTGGCC CCATCGACGG
    GACCCGAGAA GGCCAGTGGA GGGCGGCAGT GAGGTGGCGG CGGTCCCCGG TGAAGGGTCG
    AGTAAGCACA AGGACTGGCA CCGGCAGGGC CTGCGAAGAC CCTCCATCCT CCCCGAGAGC
    CCTGTAGACA CAAGAGGTCC CGCCATGGAA GGATCCCCTG GCCTGTCGGA CGCCATTGTT
    TTCCGGGAGA AAAAGCCAAA GGAGGTGATG GGAAACTTTT CAAGACGGTG CTCCAAGCTT
    ATCAACTCCT CTCAGCTGCT TTACCAGGAG TACAGTGATG TGTTTCTGAA CAAGGAGATT
    CAGAGCCAGC AGCGGTTGGA CAGCCTGACG GAATCACCGG GGCCCATCTC GCCCCGGCAG
    CCCCGAAAGG CCCTTGTCTC CTCGGAGTCC TACCTGCGTC GCCTCTCCAT GGCCTCCAGC
    GGCTCCCTGT GGCAGGAGAT CCCTGTGGTG CGCAATAGCA CCGTGCTGCT CTCCATGACC
    CATGAAGACC AGAAGCTCCA GGAGGCCAAA TTTGAGCTGA TTGTGTCAGA GGCCTCCTAC
    CTGCGCAGTT TGCATATAGC TGTGGATCAC TTCCAACATT CAGAGCAGCT CCGGGCCACC
    CTTTCCAACC AGGATCACCA ATGGCTCTTC TCTCGTTTGC AGGATGTGCG TGACGTCAGC
    ACCATGTTCC TTTCTGACCT GGAAGAGAAC TTTGAGAACA ACATCTTCAC CTTCCAAGTG
    TGTGATGTGG TCCTGAACCA TGCCCCCAAC TTCCGTCGGG TCTACCTGCC TTATGTCACC
    AACCAGACCT ACCAGGAACG CACCTTCCAA AGCCTGCTGA ACAGCAACAG CAGTTTCCGA
    GAGGTCCTGG AGAAGCTGGA GAGTGCCCCC ATCTGCCAGC GCCTTTCCCT CAAGTCCTTC
    CTGATTCTGC CTTTCCAGCG CATCACCCGT CTCAAGCTCC TGCTCCAGAA CATTCTGAAG
    AGAACACAGC CCGGCTCCGC AGAGGAAGCA GAGGCCACGA AAGCACACCA TGCCCTGGAG
    GAGCTGATCC GAGACTGCAA TAACAATGTC CAGAGGATGC GACGGACGGA GGAGCTGATC
    TACCTGAGCC AGAAGATTGA GTTTGAGTGC AAAATATTCC CGCTCATTTC ACAATCGCGC
    TGGCTGGTGA AGAGTGGGGA GCTGACGGCC CTGGAGCTCA GCGTCTCCCA GGCGCTGCGG
    AGGAAGCTGA CCACCCGCCC GGTCCACCTG CACCTCTTCA ACGACTGTCT GTTGCTGTCT
    CGGCCCCGAG AGGGTAGCCG ATTCCTGGTA TTCGACCATG CTCCCTTCTC TGCCGTCCGT
    GGGGAAAAGT GTGAAATGAA GCTGCATGGA GCTCACAAAA ACCTCTTCCG ACTCTTCCTG
    CGGCGCAACG CCCAGGGCTC CCAGGCCGAG TTCCTCCTCC GCACAGAGAC CCAAAGTGAA
    AAGCTTCGCT GGATCTCAGC CTTGGCTATG CCGAGGGAGG AGTTGGACCT TCTGGAGTGT
    TACGACTCCC CACAAGTGCA GTGCCTTAGA GCCTACAAAC CCCGAGAGAA TGACGAGTTG
    GCGCTCGAGA AGGCAGACGT GGTGATGGTG ACCCAGCAGA GCAGTGATGG CTGGCTGGAA
    GGCGTGAGAC TGTCCGATGG GGAGCAAGGC TGGTTCCCTC TACAACAAGT AGAGTTCATC
    TCCAACCCAG AGGTCCGCGC ACGGAACCTG AAGGAAGCCC ACCGAGTCAA GACTGCCAAG
    CTACAGCTGG TGGAGCAGCA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18861
    Clone ID Related Accession (Same CDS sequence) XM_005205839.2 , NM_001110075.2 , XM_005205839.3
    Accession Version NM_001110075.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000004.1, EE350207.1, CB440533.1, BI539326.1 and BF042105.1. On Feb 14, 2012 this sequence version replaced NM_001110075.1. Sequence Note: This RefSeq record was created from transcripts of different breeds because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001103545.2
    CDS1..4755
    Translation

    Target ORF information:

    RefSeq Version NM_001110075.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001110075.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18861
    Clone ID Related Accession (Same CDS sequence) XM_005205839.2 , NM_001110075.2 , XM_005205839.3
    Accession Version XM_005205839.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005205839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205896.1
    CDS118..4872
    Translation

    Target ORF information:

    RefSeq Version XM_005205839.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205839.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18861
    Clone ID Related Accession (Same CDS sequence) XM_005205839.2 , NM_001110075.2 , XM_005205839.3
    Accession Version XM_005205839.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205896.1
    CDS118..4872
    Translation

    Target ORF information:

    RefSeq Version XM_005205839.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205839.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCGG AGGAGCCCCA GCATGGAGCC TCGACTCCCA TCCCTGCCAG AGCAGAATTC 
    CCTGTTATCT GGGCACCTCC CATGAGGAGC AGCAAGACCC CTGCCTTGGA GCCTGCAGCC
    GAAGAGGGTG AGGACCCGCG GTGTCAGTGG GCTGAGGGCT GCACACTCTT GGAGACCCAG
    CAGAGACACG TGAGGGATGC GAATGACCGT GCAGCTGAGA GAATGACCTC TCTCCTCAAC
    GAAGCCTCTC CAGAGGTGGA GACCGACCAG GAAACCCTGA TGGCTGAGGC CTGTGATGAT
    ACCCCCTCAT GCCAGGAGGC CGGGCCCTGG AGCCTGGAAG ATAGACAGGC AAGGACGCCA
    GCTTCCCTGG ATTTCCTGAC CTGCCCTGAC CAGGGTGACT GTCTGGACAC GGCCTCGGTA
    TCCAGGGATC TGGACACCAG CGTGGAGGTG GACTTTAAAC CAGAACTCAC GTCTTTGATA
    TTGGAAACGG GACAAGCAGA AGGGAAGGAG GAAACATCCT CCAACGCCTA TGCCCGGACC
    AGACACAGGC CTTCCTGTGA TGACGAGCAC CCGGCTGAGA CCAGCCGGCC CCTGGAGGAC
    TCGGAGTGTG GACCTGCCAG GCAGGAAAGT CCAGGCCTCG TGTGTCTCCA GGGAACTGAG
    GGGCTGGAGG AAGGGGGACC TCAGGGAGAG GCTGCTGGCT CTGCTGGGCT TTCCGGGGCT
    CCAGAGCAGA TGGGAGAGCA GGTTAAAGGT GCAGAGGAAG AAGGGAGACA GAAACAGCAG
    CAGATGCAAA ATGACATGGT GCTTGAAGAT CCAGGAGAAA GAGAGGGGTG GTGCTGCGGG
    GAGGAGTTGG GGGGTCTGAG TTATGGGGAG TGGGACTGGA GGACTTGGAG CCACGGGGAT
    CCAGAACAAG GGGAGCAGAA AGGGAGGGAG TTAACAGGGC CAGAGGAGAA TGAGGTGGGT
    GCTCAGGACG GGGAGAATCA AAGCTCAGCA AGTAAGTCAG AGAAAGGAAG TGGAAAGCCC
    GAAGATCCGG GTGCACAGTG GGAAGCCAAG GCAGCAGAGG GGCAGGAGGA GGAAGTGGGG
    TCACTGGAGG AGGAACCAGG GGGCGGGGAG GGGGACACAC CCAGCCGAGG AGGAGAAGAG
    AGCTGCATAG AACAAGGAGA GCGTGAGGGT CCCACTGCGC TGGCTCTGGT CGCCCCTGAG
    GTCTCTCCTC CCCGTGACCT GTCTCTAGAG GCCTCTTCTC CCCTGAGCAG GATCCCGGGG
    ACTCAAACAG AGCCTGGAGC CGAGGTTCCA TCCCCCATAG CTCCGAGTCC TGCCCCAGAA
    CCCACAGGAT GCTCCCACCA GCCCTTTTCT TTACCTGGTT CCTGTCCTGC CGAGGAGTCC
    CTGGACCCAG AAACTGCTCA TCGCCACCAG CAAGAGGGCT CTGAGCTGGG GCAGGAGACC
    GCACATGGCG TGGGGGCAGA GGTGGTCTCT GCCTACAGTC CCTCTGCGAC CCCTCCAAGG
    AGCCCAGAGG CAGCTGCACC CAGTCCTCCT GAAGGGCCCC CTGGCACGGC TGCCACACCG
    ATGGCCCGTC CCCTGGCTGC CTCTCCCAGG AGGGAGCCTC CTGTCCTTGC CTGTTCTCCA
    GAAACCTCCA GAGCCTCTTG CCCGACTGAC AGTCCATCTC CCCATGGGGC TCCTAAGACT
    CCCCCAGCAG CCTGCTACGG CTTCCCCTTG GCGGGCCGGG CCAACCCCCC GGGGTCCCTG
    GGCAGCCCCA CCGGAGCGGT CCAGCCCGTG AGGAGCAACT CCTTCCCTGG GTCTCATGGG
    ACAGAGCCAG CCCCACACCT GGCGGGAATA TCCCTGTCCT CCTCCCACTC AGAGTTGCCC
    CAGAGGCCTC CCAAACCTGT CATCTATGGC TCTGTGCTCC CAAGAAGGGG CAGGAGAACT
    GTAAGGGACT GTGCCATCAT TCCGGAAGCC TCGAGTTCAC CACCCACTCC GGGGCAGGAC
    TTTGGAGAAC TGGCTTCAAA TCCAGCCACG GCCGGCAGCC CCCCTGACAG TCAGCCGTGG
    GGACCCACGA AGCACTGGGC CTTTGCCCCC GGGTCTCCTG CCTGTGGCTC CTCCCCAGCC
    CCTGCAGCCC CCATGGACCT GATGATCCAT GAACCTCCGC CCCTGCCTCC CCTGGAGAGA
    AGGCACGTCC AGCCCTCCAC GGTGGAGCCA GACGGCCATC CCCCTGCGGT GATCCCCATG
    CTGAAGCGGT ACAGCCATCC TCCACCACTC ACCCTGGGTC CAGGGCTCCA CGGCCCCCCC
    AGGGTACCCC CAGCCCACAT TCCTGACCCC TTGGTGGCGA GGGAGCACCG ACCGCTGCCC
    TCCACGCCAG ACGCGCCACC CCACACTCAG CACTCTGTCT CTTCCAGGCA GAGATACAAC
    AAGCCGTTAC CCCCCACCCC CGACGCCCCC CAGCCCCACC ACCCCCTTGT CTTGTCGAGC
    ATCTCAAGGA TCTACAAGCC TCTCCCTCCT GTCCCTATCC TGGATTCTCC TACTGAACCA
    CCCCCTCTGC CCCCAAAGTC CAAGGGGAAG GGCAGGAGCT CGCAGGGAGG ACTCGTGAAC
    TCAGGGGATC AAGGCAGGCC GAGGCCTGTT TGTCAAGAGT GGACCGTCTC CACCCCACCT
    TCTGCAGGAC GGGTCTCCTG GCCTCCAGCC ACAGGCCGGT CAACCGACCC TTTGGCTTCC
    GTCGGCAGGT GTAAGAGTGA TACGCCCCCG GGCCTGGCTT TCAGTAACAT GACGGCCCTT
    CTACACCCAT CTTCCCCGAC CACCCCGTGG ACTCCAGAGG TCCAGCGACC CACCTCTGAA
    CCCGGGCTCT CAGAAGAGTC TGAAGCCCCT GCCAGAGGGT CTTGGAGAAG AACAGCCCCC
    CAGGAAGGAA GCAATGGCCT GAGGAGGTCA GAGGTAGGCC CGGTGAGGCA GCCGGAGAAA
    TCGGGCCACA TCCTCCTGGA GAAGGCATCG AGCTGGCCCC ATCGACGGGA CCCGAGAAGG
    CCAGTGGAGG GCGGCAGTGA GGTGGCGGCG GTCCCCGGTG AAGGGTCGAG TAAGCACAAG
    GACTGGCACC GGCAGGGCCT GCGAAGACCC TCCATCCTCC CCGAGAGCCC TGTAGACACA
    AGAGGTCCCG CCATGGAAGG ATCCCCTGGC CTGTCGGACG CCATTGTTTT CCGGGAGAAA
    AAGCCAAAGG AGGTGATGGG AAACTTTTCA AGACGGTGCT CCAAGCTTAT CAACTCCTCT
    CAGCTGCTTT ACCAGGAGTA CAGTGATGTG TTTCTGAACA AGGAGATTCA GAGCCAGCAG
    CGGTTGGACA GCCTGACGGA ATCACCGGGG CCCATCTCGC CCCGGCAGCC CCGAAAGGCC
    CTTGTCTCCT CGGAGTCCTA CCTGCGTCGC CTCTCCATGG CCTCCAGCGG CTCCCTGTGG
    CAGGAGATCC CTGTGGTGCG CAATAGCACC GTGCTGCTCT CCATGACCCA TGAAGACCAG
    AAGCTCCAGG AGGCCAAATT TGAGCTGATT GTGTCAGAGG CCTCCTACCT GCGCAGTTTG
    CATATAGCTG TGGATCACTT CCAACATTCA GAGCAGCTCC GGGCCACCCT TTCCAACCAG
    GATCACCAAT GGCTCTTCTC TCGTTTGCAG GATGTGCGTG ACGTCAGCAC CATGTTCCTT
    TCTGACCTGG AAGAGAACTT TGAGAACAAC ATCTTCACCT TCCAAGTGTG TGATGTGGTC
    CTGAACCATG CCCCCAACTT CCGTCGGGTC TACCTGCCTT ATGTCACCAA CCAGACCTAC
    CAGGAACGCA CCTTCCAAAG CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG
    AAGCTGGAGA GTGCCCCCAT CTGCCAGCGC CTTTCCCTCA AGTCCTTCCT GATTCTGCCT
    TTCCAGCGCA TCACCCGTCT CAAGCTCCTG CTCCAGAACA TTCTGAAGAG AACACAGCCC
    GGCTCCGCAG AGGAAGCAGA GGCCACGAAA GCACACCATG CCCTGGAGGA GCTGATCCGA
    GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACGGAGG AGCTGATCTA CCTGAGCCAG
    AAGATTGAGT TTGAGTGCAA AATATTCCCG CTCATTTCAC AATCGCGCTG GCTGGTGAAG
    AGTGGGGAGC TGACGGCCCT GGAGCTCAGC GTCTCCCAGG CGCTGCGGAG GAAGCTGACC
    ACCCGCCCGG TCCACCTGCA CCTCTTCAAC GACTGTCTGT TGCTGTCTCG GCCCCGAGAG
    GGTAGCCGAT TCCTGGTATT CGACCATGCT CCCTTCTCTG CCGTCCGTGG GGAAAAGTGT
    GAAATGAAGC TGCATGGAGC TCACAAAAAC CTCTTCCGAC TCTTCCTGCG GCGCAACGCC
    CAGGGCTCCC AGGCCGAGTT CCTCCTCCGC ACAGAGACCC AAAGTGAAAA GCTTCGCTGG
    ATCTCAGCCT TGGCTATGCC GAGGGAGGAG TTGGACCTTC TGGAGTGTTA CGACTCCCCA
    CAAGTGCAGT GCCTTAGAGC CTACAAACCC CGAGAGAATG ACGAGTTGGC GCTCGAGAAG
    GCAGACGTGG TGATGGTGAC CCAGCAGAGC AGTGATGGCT GGCTGGAAGG CGTGAGACTG
    TCCGATGGGG AGCAAGGCTG GTTCCCTCTA CAACAAGTAG AGTTCATCTC CAACCCAGAG
    GTCCGCGCAC GGAACCTGAA GGAAGCCCAC CGAGTCAAGA CTGCCAAGCT ACAGCTGGTG
    GAGCAGCAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.