ACTA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTA2
Entrez Gene ID	515610
Full Name	actin, alpha 2, smooth muscle, aorta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005225364.3	XP_005225421.1	actin, aortic smooth muscle isoform X1
NM_001034502.1	NP_001029674.1	actin, aortic smooth muscle

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Acta2	NP_112266.1
Gallus gallus (chicken)	ACTA2	NP_001026400.1
Xenopus tropicalis (tropical clawed frog)	acta1	NP_001006709.1
Homo sapiens (human)	ACTA2	NP_001604.1
Canis lupus familiaris (dog)	ACTA2	XP_534781.2
Bos taurus (cattle)	ACTA2	NP_001029674.1
	ACTA2	XP_002805789.1
Danio rerio (zebrafish)	acta1a	NP_001017750.1
Oryza sativa (rice)	Os12g0163700	NP_001066233.1
Xenopus tropicalis (tropical clawed frog)	MGC75582	NP_989355.1
Pan troglodytes (chimpanzee)	ACTA2	XP_001142014.1
Mus musculus (house mouse)	Acta2	NP_031418.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Ishiwata H, Katsuma S, Kizaki K, Patel OV, Nakano H, Takahashi T, Imai K, Hirasawa A, Shiojima S, Ikawa H, Suzuki Y, Tsujimoto G, Izaike Y, Todoroki J, Hashizume K
Molecular reproduction and development65(1)9-18(2003 May)

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs.
Takasuga A, Hirotsune S, Itoh R, Jitohzono A, Suzuki H, Aso H, Sugimoto Y
Nucleic acids research29(22)E108(2001 Nov)

The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.
Vandekerckhove J, Weber K
Differentiation; research in biological diversity14(3)123-33(1979)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACTA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa33441	NM_001034502.1 Latest version!	Bos taurus actin alpha 2, smooth muscle (ACTA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa33441	XM_005225364.3 Latest version!	Bos taurus actin, alpha 2, smooth muscle, aorta (ACTA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTA2 ( NM_001034502.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029674.1 Bos taurus actin alpha 2, smooth muscle (ACTA2), mRNA.

Price & Availability↑

CloneID	OBa33441
Clone ID Related Accession (Same CDS sequence)	XM_005225364.3 , NM_001034502.1
Accession Version	NM_001034502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	actin, aortic smooth muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102699.1. On Oct 24, 2005 this sequence version replaced XM_593657.2. ##Evidence-Data-START## Transcript exon combination :: BC102699.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGTGCGAGG AGGAGGACAG CACCGCCTTG GTGTGTGACA ATGGCTCTGG GCTCTGTAAG 
GCCGGCTTTG CGGGGGATGA TGCTCCCAGG GCTGTTTTCC CATCTATCGT GGGACGTCCT 
CGACATCAGG GAGTGATGGT GGGAATGGGA CAGAAAGACA GCTACGTGGG TGATGAAGCA 
CAAAGCAAAA GAGGAATCCT GACCCTGAAG TACCCGATAG AGCACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCTTTCTACA ATGAGCTTCG TGTTGCCCCT 
GAAGAGCATC CAACCCTTCT CACCGAGGCG CCCCTGAACC CCAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACTTTCAAT GTCCCAGCCA TGTATGTGGC TATTCAGGCG 
GTGCTGTCCC TCTACGCCTC TGGACGCACA ACTGGCATTG TGCTGGACTC TGGAGATGGT 
GTCACCCACA ACGTGCCCAT CTATGAGGGC TACGCCCTGC CCCACGCCAT CATGCGTCTG 
GACCTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACCGA GCGAGGCTAT 
TCCTTCGTGA CCACCGCTGA GCGTGAGATT GTCCGGGACA TCAAGGAGAA ACTGTGCTAC 
GTAGCCCTGG ACTTTGAGAA TGAGATGGCC ACTGCCGCAT CCTCCTCCTC CCTCGAGAAG 
AGCTATGAGC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAGCGTTT CCGCTGCCCT 
GAGACCCTGT TCCAGCCCTC GTTCATCGGG ATGGAGTCTG CTGGCATCCA CGAGACCACG 
TACAACAGCA TCATGAAGTG TGATATTGAT ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCCGGGG GTACCACCAT GTACCCCGGC ATTGCTGACC GCATGCAGAA GGAGATCACG 
GCCCTGGCCC CCAGCACCAT GAAGATCAAG ATCATCGCCC CTCCCGAGCG CAAGTACTCT 
GTCTGGATTG GTGGCTCCAT CCTGGCCTCT TTGTCCACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ACGACGAAGC CGGGCCATCC ATCGTCCACC GCAAATGTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029674.1
CDS	68..1201
Translation	MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001034502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin alpha 2, smooth muscle (ACTA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034502.1

ORF Insert Sequence:

ATGTGCGAGG AGGAGGACAG CACCGCCTTG GTGTGTGACA ATGGCTCTGG GCTCTGTAAG 
GCCGGCTTTG CGGGGGATGA TGCTCCCAGG GCTGTTTTCC CATCTATCGT GGGACGTCCT 
CGACATCAGG GAGTGATGGT GGGAATGGGA CAGAAAGACA GCTACGTGGG TGATGAAGCA 
CAAAGCAAAA GAGGAATCCT GACCCTGAAG TACCCGATAG AGCACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCTTTCTACA ATGAGCTTCG TGTTGCCCCT 
GAAGAGCATC CAACCCTTCT CACCGAGGCG CCCCTGAACC CCAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACTTTCAAT GTCCCAGCCA TGTATGTGGC TATTCAGGCG 
GTGCTGTCCC TCTACGCCTC TGGACGCACA ACTGGCATTG TGCTGGACTC TGGAGATGGT 
GTCACCCACA ACGTGCCCAT CTATGAGGGC TACGCCCTGC CCCACGCCAT CATGCGTCTG 
GACCTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACCGA GCGAGGCTAT 
TCCTTCGTGA CCACCGCTGA GCGTGAGATT GTCCGGGACA TCAAGGAGAA ACTGTGCTAC 
GTAGCCCTGG ACTTTGAGAA TGAGATGGCC ACTGCCGCAT CCTCCTCCTC CCTCGAGAAG 
AGCTATGAGC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAGCGTTT CCGCTGCCCT 
GAGACCCTGT TCCAGCCCTC GTTCATCGGG ATGGAGTCTG CTGGCATCCA CGAGACCACG 
TACAACAGCA TCATGAAGTG TGATATTGAT ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCCGGGG GTACCACCAT GTACCCCGGC ATTGCTGACC GCATGCAGAA GGAGATCACG 
GCCCTGGCCC CCAGCACCAT GAAGATCAAG ATCATCGCCC CTCCCGAGCG CAAGTACTCT 
GTCTGGATTG GTGGCTCCAT CCTGGCCTCT TTGTCCACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ACGACGAAGC CGGGCCATCC ATCGTCCACC GCAAATGTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTA2 ( XM_005225364.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225421.1 Bos taurus actin, alpha 2, smooth muscle, aorta (ACTA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa33441
Clone ID Related Accession (Same CDS sequence)	XM_005225364.3 , NM_001034502.1
Accession Version	XM_005225364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin, aortic smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGAGG AGGAGGACAG CACCGCCTTG GTGTGTGACA ATGGCTCTGG GCTCTGTAAG 
GCCGGCTTTG CGGGGGATGA TGCTCCCAGG GCTGTTTTCC CATCTATCGT GGGACGTCCT 
CGACATCAGG GAGTGATGGT GGGAATGGGA CAGAAAGACA GCTACGTGGG TGATGAAGCA 
CAAAGCAAAA GAGGAATCCT GACCCTGAAG TACCCGATAG AGCACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCTTTCTACA ATGAGCTTCG TGTTGCCCCT 
GAAGAGCATC CAACCCTTCT CACCGAGGCG CCCCTGAACC CCAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACTTTCAAT GTCCCAGCCA TGTATGTGGC TATTCAGGCG 
GTGCTGTCCC TCTACGCCTC TGGACGCACA ACTGGCATTG TGCTGGACTC TGGAGATGGT 
GTCACCCACA ACGTGCCCAT CTATGAGGGC TACGCCCTGC CCCACGCCAT CATGCGTCTG 
GACCTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACCGA GCGAGGCTAT 
TCCTTCGTGA CCACCGCTGA GCGTGAGATT GTCCGGGACA TCAAGGAGAA ACTGTGCTAC 
GTAGCCCTGG ACTTTGAGAA TGAGATGGCC ACTGCCGCAT CCTCCTCCTC CCTCGAGAAG 
AGCTATGAGC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAGCGTTT CCGCTGCCCT 
GAGACCCTGT TCCAGCCCTC GTTCATCGGG ATGGAGTCTG CTGGCATCCA CGAGACCACG 
TACAACAGCA TCATGAAGTG TGATATTGAT ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCCGGGG GTACCACCAT GTACCCCGGC ATTGCTGACC GCATGCAGAA GGAGATCACG 
GCCCTGGCCC CCAGCACCAT GAAGATCAAG ATCATCGCCC CTCCCGAGCG CAAGTACTCT 
GTCTGGATTG GTGGCTCCAT CCTGGCCTCT TTGTCCACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ACGACGAAGC CGGGCCATCC ATCGTCCACC GCAAATGTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225421.1
CDS	736..1869
Translation	MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_005225364.3
Organism	Bos taurus(cattle)
Definition	Bos taurus actin, alpha 2, smooth muscle, aorta (ACTA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225364.3

ORF Insert Sequence:

ATGTGCGAGG AGGAGGACAG CACCGCCTTG GTGTGTGACA ATGGCTCTGG GCTCTGTAAG 
GCCGGCTTTG CGGGGGATGA TGCTCCCAGG GCTGTTTTCC CATCTATCGT GGGACGTCCT 
CGACATCAGG GAGTGATGGT GGGAATGGGA CAGAAAGACA GCTACGTGGG TGATGAAGCA 
CAAAGCAAAA GAGGAATCCT GACCCTGAAG TACCCGATAG AGCACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCTTTCTACA ATGAGCTTCG TGTTGCCCCT 
GAAGAGCATC CAACCCTTCT CACCGAGGCG CCCCTGAACC CCAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACTTTCAAT GTCCCAGCCA TGTATGTGGC TATTCAGGCG 
GTGCTGTCCC TCTACGCCTC TGGACGCACA ACTGGCATTG TGCTGGACTC TGGAGATGGT 
GTCACCCACA ACGTGCCCAT CTATGAGGGC TACGCCCTGC CCCACGCCAT CATGCGTCTG 
GACCTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACCGA GCGAGGCTAT 
TCCTTCGTGA CCACCGCTGA GCGTGAGATT GTCCGGGACA TCAAGGAGAA ACTGTGCTAC 
GTAGCCCTGG ACTTTGAGAA TGAGATGGCC ACTGCCGCAT CCTCCTCCTC CCTCGAGAAG 
AGCTATGAGC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAGCGTTT CCGCTGCCCT 
GAGACCCTGT TCCAGCCCTC GTTCATCGGG ATGGAGTCTG CTGGCATCCA CGAGACCACG 
TACAACAGCA TCATGAAGTG TGATATTGAT ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCCGGGG GTACCACCAT GTACCCCGGC ATTGCTGACC GCATGCAGAA GGAGATCACG 
GCCCTGGCCC CCAGCACCAT GAAGATCAAG ATCATCGCCC CTCCCGAGCG CAAGTACTCT 
GTCTGGATTG GTGGCTCCAT CCTGGCCTCT TTGTCCACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ACGACGAAGC CGGGCCATCC ATCGTCCACC GCAAATGTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Molecular reproduction and development65(1)9-18(2003 May)
Ishiwata H,Katsuma S,Kizaki K,Patel OV,Nakano H,Takahashi T,Imai K,Hirasawa A,Shiojima S,Ikawa H,Suzuki Y,Tsujimoto G,Izaike Y,Todoroki J,Hashizume K

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs.
Nucleic acids research29(22)E108(2001 Nov)
Takasuga A,Hirotsune S,Itoh R,Jitohzono A,Suzuki H,Aso H,Sugimoto Y

The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.
Differentiation; research in biological diversity14(3)123-33(1979)
Vandekerckhove J,Weber K

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ACTA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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