ABCG5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCG5
Entrez Gene ID	515536
Full Name	ATP binding cassette subfamily G member 5
General protein information	Preferred Names ATP binding cassette subfamily G member 5 Names ATP-binding cassette sub-family G member 5 ATP-binding cassette, sub-family G (WHITE), member 5 (sterolin 1)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024547.2	NP_001019718.2	ATP-binding cassette sub-family G member 5
NM_001024547.1	NP_001019718.1	ATP-binding cassette sub-family G member 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG5	NP_071881.1
Canis lupus familiaris (dog)	ABCG5	XP_538475.1
Rattus norvegicus (Norway rat)	Abcg5	NP_446206.2
Danio rerio (zebrafish)	abcg5	NP_001122162.1
Bos taurus (cattle)	ABCG5	NP_001019718.1
Mus musculus (house mouse)	Abcg5	NP_114090.1
Drosophila melanogaster (fruit fly)	CG11069	NP_651307.2
Xenopus tropicalis (tropical clawed frog)	abcg5	NP_001120613.1
Pan troglodytes (chimpanzee)	ABCG5	XP_003309098.1
	ABCG5	XP_001111277.1
Gallus gallus (chicken)	ABCG5	XP_419457.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002051	XP_320996.5

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification, sequence analysis and mRNA tissue distribution of the bovine sterol transporters ABCG5 and ABCG8.
Viturro E, Farke C, Meyer HH, Albrecht C
Journal of dairy science89(2)553-61(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

high expression levels of both ATP-binding cassette sub-family G member 5 and 8 (ABCG5 and ABCG8) were present in bovine liver and digestive tract samples, and in the mammary gland
Title: Identification, sequence analysis and mRNA tissue distribution of the bovine sterol transporters ABCG5 and ABCG8.

The following ABCG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37268	NM_001024547.1 Latest version!	Bos taurus ATP-binding cassette, sub-family G (WHITE), member 5 (ABCG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa239204	NM_001024547.2 Latest version!	Bos taurus ATP binding cassette subfamily G member 5 (ABCG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCG5 ( NM_001024547.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019718.1 Bos taurus ATP-binding cassette, sub-family G (WHITE), member 5 (ABCG5), mRNA.

Price & Availability↑

CloneID	OBa37268
Clone ID Related Accession (Same CDS sequence)	NM_001024547.1
Accession Version	NM_001024547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	ATP-binding cassette sub-family G member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ001133.1. On May 29, 2005 this sequence version replaced XM_593572.1. ##Evidence-Data-START## Transcript exon combination :: DQ001133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGAAC TCCCCTTTTT GACTCTTGGG GAGTCCTCAG AACTCCAAGT AAACAGAAGC 
TCTCAGTGCT CCCTAGAGGA GGTTCCTGCA AAGCCTCGAC ACAGCCTGGG CATTTTCCAT 
GCCTCGTACA GTGTCAGCCA CCATGTGGGG CCCTGGTGGG ACATCGCATC TCGCCGACAG 
AAGTGGACCA GGCAGATCCT CAAAGATGTC TCCTTGTACG TCGAGAGCGG GCAGATCATG 
TGCATCTTAG GGAGCTCAGG CTCAGGGAAA ACCACGCTGC TGGACGCCAT GTCCGGGAGG 
CTGCGGCGCA CAGGGACCTT GCTTGGGGAG GTGTTCGTGA ACGGCCAGGA GCTGCACAGG 
GAGCAGTTCC AGGACTGCTT CTCCTACGTC CTGCAGAGCG ACACTCTGCT GAGCAACCTC 
ACCGTCCGCG AGACGCTGAA CTACACGGCA CTCCTGGCCA TCCGCCGCGG TTCCCAGAGC 
TTCTTCCAGA AGAAGGTGGA GGCAGTCATG ACGGAGCTGA GTTTGAGCCA CGTGGCAGAT 
CAACTGATTG GCAACTACAG CTTTGGGGGA ATTTCCCATG GCGAGAGGCG CCGAGTCTCC 
ATTGCAGCCC AGCTCCTCCA GGATCCCAAG GTCATGCTGT TTGATGAGCC AACCACAGGT 
CTGGACTGCA TGACTGCAAA TCAGATCGTC GTCCTCCTAG CAGAGCTGGC TCGCAGGGAC 
CGCATTGTGA TCCTCACCAT CCACCAGCCC CGCTCTGAGC TCTTCCAGCT CTTTGACAAA 
ATCGCCATCC TGAGCTGCGG AGAGCTGGTT TTCTGTGGGA CACCGGTGGA AATGCTTGAT 
TTCTTCAGTG GCTGTGGTTA CCCTTGTCCT GAACATTCAA ACCCTTTTGA TTTTTATATG 
GACCTGACAT CAGTGGATAC CCAAAGCAAA GAACGAGAAA TAGAAACCTA CAGAAGAGTC 
CAGATGATTG AAGTTGCCTA CAGAGAATCA GCAATGTATC ACAAAACCAT GGAGGCTATT 
GAAAGAACAA AACATCTGAA AACATTACCA AAGATTCCTT TCAAAACCAG AGATTCTCCT 
GGAGCCCTCT CTAAACTGTG TGTTCTCTTG AGGAGAGTGA TGAGAAACTT ACTGAGAAAT 
AAGCTGGCGG TGACGATGCG TCTTACTCAG AATCTGATCA TGGGTCTATT CGTCATTTTC 
TTCCTTCTAC GACTGGGGAA GGATGTGCTG AAGGGTGCCG TCCAGGATCG TGTGGGTCTC 
CTGTACCAGT TTGTGGGTGC CATGCCGTAC ACAGGCATGC TCAATGCTGT GACCCTGTTT 
CCTGTGCTGC GTGCCGTCAG TGACCAGGAG AGCCAGGATG GCCTGTACCA CAAGTGGCAG 
ATGCTACTGG CCTACATGCT GCACGTGCTT CCCTTCAGCG TCACTGCCAC CCTCGTATGC 
TGCAGTGTGT GCTACTGGAC TCTGGCCTTA TACCCCGAGG CTGCCAGATT TGCATATTTC 
TCTGTTGCTC TCTTGGCCCC CCACTTAATT GGTGAATTTC TAAGCCTTGT GCTACTTGGC 
GTGATCCAAA ACCCAAATGT GGTCAACAGC GTGGTGGCCC TGCTCTCCAT TGCTGGGGTG 
CTTATGGGCT CTGGCTTCGT GAGAAACATA CAAGAAATGC CCATTCCTTT TAAAATCCTC 
GGTTATTTTA CATTCCAAAA ATATTGCTCT GAGATTCTCG TAGTCAATGA GTTCTATGGA 
CAGAATTTCA CTTGTGGCAA CTCAAACGCC TCTGTGTCAG CTAACCCAAT GTGTACCATC 
ACTCAAGGAG CTCAGTTCAT TGAGAGAAAC TGCCCAGGTG CAACATCCAG ATTCACAGCA 
AACTTTCTCA TTTTGTATTC ATTTATCCCA GCTCTTGTCA TCCTAGGAAT AATTGTGTTT 
AAAATAAGGG ATTATCTCAT TAGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019718.1
CDS	137..2086
Translation	MGELPFLTLGESSELQVNRSSQCSLEEVPAKPRHSLGIFHASYSVSHHVGPWWDIASRRQKWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLRRTGTLLGEVFVNGQELHREQFQDCFSYVLQSDTLLSNLTVRETLNYTALLAIRRGSQSFFQKKVEAVMTELSLSHVADQLIGNYSFGGISHGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLAELARRDRIVILTIHQPRSELFQLFDKIAILSCGELVFCGTPVEMLDFFSGCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETYRRVQMIEVAYRESAMYHKTMEAIERTKHLKTLPKIPFKTRDSPGALSKLCVLLRRVMRNLLRNKLAVTMRLTQNLIMGLFVIFFLLRLGKDVLKGAVQDRVGLLYQFVGAMPYTGMLNAVTLFPVLRAVSDQESQDGLYHKWQMLLAYMLHVLPFSVTATLVCCSVCYWTLALYPEAARFAYFSVALLAPHLIGEFLSLVLLGVIQNPNVVNSVVALLSIAGVLMGSGFVRNIQEMPIPFKILGYFTFQKYCSEILVVNEFYGQNFTCGNSNASVSANPMCTITQGAQFIERNCPGATSRFTANFLILYSFIPALVILGIIVFKIRDYLISR

Target ORF information:

RefSeq Version	NM_001024547.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP-binding cassette, sub-family G (WHITE), member 5 (ABCG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024547.1

ORF Insert Sequence:

ATGGGCGAAC TCCCCTTTTT GACTCTTGGG GAGTCCTCAG AACTCCAAGT AAACAGAAGC 
TCTCAGTGCT CCCTAGAGGA GGTTCCTGCA AAGCCTCGAC ACAGCCTGGG CATTTTCCAT 
GCCTCGTACA GTGTCAGCCA CCATGTGGGG CCCTGGTGGG ACATCGCATC TCGCCGACAG 
AAGTGGACCA GGCAGATCCT CAAAGATGTC TCCTTGTACG TCGAGAGCGG GCAGATCATG 
TGCATCTTAG GGAGCTCAGG CTCAGGGAAA ACCACGCTGC TGGACGCCAT GTCCGGGAGG 
CTGCGGCGCA CAGGGACCTT GCTTGGGGAG GTGTTCGTGA ACGGCCAGGA GCTGCACAGG 
GAGCAGTTCC AGGACTGCTT CTCCTACGTC CTGCAGAGCG ACACTCTGCT GAGCAACCTC 
ACCGTCCGCG AGACGCTGAA CTACACGGCA CTCCTGGCCA TCCGCCGCGG TTCCCAGAGC 
TTCTTCCAGA AGAAGGTGGA GGCAGTCATG ACGGAGCTGA GTTTGAGCCA CGTGGCAGAT 
CAACTGATTG GCAACTACAG CTTTGGGGGA ATTTCCCATG GCGAGAGGCG CCGAGTCTCC 
ATTGCAGCCC AGCTCCTCCA GGATCCCAAG GTCATGCTGT TTGATGAGCC AACCACAGGT 
CTGGACTGCA TGACTGCAAA TCAGATCGTC GTCCTCCTAG CAGAGCTGGC TCGCAGGGAC 
CGCATTGTGA TCCTCACCAT CCACCAGCCC CGCTCTGAGC TCTTCCAGCT CTTTGACAAA 
ATCGCCATCC TGAGCTGCGG AGAGCTGGTT TTCTGTGGGA CACCGGTGGA AATGCTTGAT 
TTCTTCAGTG GCTGTGGTTA CCCTTGTCCT GAACATTCAA ACCCTTTTGA TTTTTATATG 
GACCTGACAT CAGTGGATAC CCAAAGCAAA GAACGAGAAA TAGAAACCTA CAGAAGAGTC 
CAGATGATTG AAGTTGCCTA CAGAGAATCA GCAATGTATC ACAAAACCAT GGAGGCTATT 
GAAAGAACAA AACATCTGAA AACATTACCA AAGATTCCTT TCAAAACCAG AGATTCTCCT 
GGAGCCCTCT CTAAACTGTG TGTTCTCTTG AGGAGAGTGA TGAGAAACTT ACTGAGAAAT 
AAGCTGGCGG TGACGATGCG TCTTACTCAG AATCTGATCA TGGGTCTATT CGTCATTTTC 
TTCCTTCTAC GACTGGGGAA GGATGTGCTG AAGGGTGCCG TCCAGGATCG TGTGGGTCTC 
CTGTACCAGT TTGTGGGTGC CATGCCGTAC ACAGGCATGC TCAATGCTGT GACCCTGTTT 
CCTGTGCTGC GTGCCGTCAG TGACCAGGAG AGCCAGGATG GCCTGTACCA CAAGTGGCAG 
ATGCTACTGG CCTACATGCT GCACGTGCTT CCCTTCAGCG TCACTGCCAC CCTCGTATGC 
TGCAGTGTGT GCTACTGGAC TCTGGCCTTA TACCCCGAGG CTGCCAGATT TGCATATTTC 
TCTGTTGCTC TCTTGGCCCC CCACTTAATT GGTGAATTTC TAAGCCTTGT GCTACTTGGC 
GTGATCCAAA ACCCAAATGT GGTCAACAGC GTGGTGGCCC TGCTCTCCAT TGCTGGGGTG 
CTTATGGGCT CTGGCTTCGT GAGAAACATA CAAGAAATGC CCATTCCTTT TAAAATCCTC 
GGTTATTTTA CATTCCAAAA ATATTGCTCT GAGATTCTCG TAGTCAATGA GTTCTATGGA 
CAGAATTTCA CTTGTGGCAA CTCAAACGCC TCTGTGTCAG CTAACCCAAT GTGTACCATC 
ACTCAAGGAG CTCAGTTCAT TGAGAGAAAC TGCCCAGGTG CAACATCCAG ATTCACAGCA 
AACTTTCTCA TTTTGTATTC ATTTATCCCA GCTCTTGTCA TCCTAGGAAT AATTGTGTTT 
AAAATAAGGG ATTATCTCAT TAGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG5 ( NM_001024547.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019718.2 Bos taurus ATP binding cassette subfamily G member 5 (ABCG5), mRNA.

Price & Availability↑

CloneID	OBa239204
Clone ID Related Accession (Same CDS sequence)	NM_001024547.2
Accession Version	NM_001024547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Bos taurus(cattle)
Product	ATP-binding cassette sub-family G member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ001133.1 and CK948920.1. On Sep 4, 2018 this sequence version replaced NM_001024547.1. ##Evidence-Data-START## Transcript exon combination :: DQ001133.1, SRR5571260.33569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145427, SAMN03145482 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGAAC TCCCCTTTTT GACTCTTGGG GAGTCCTCAG AACTCCAAGT AAACAGAAGC 
TCTCAGTGCT CCCTAGAGGA GGTTCCTGCA AAGCCTCGAC ACAGCCTGGG CATTTTCCAT 
GCCTCGTACA GTGTCAGCCA CCATGTGGGG CCCTGGTGGG ACATCGCATC TCGCCGACAG 
AAGTGGACCA GGCAGATCCT CAAAGATGTC TCCTTGTACG TCGAGAGCGG GCAGATCATG 
TGCATCTTAG GGAGCTCAGG CTCAGGGAAA ACCACGCTGC TGGACGCCAT GTCCGGGAGG 
CTGCGGCGCA CAGGGACCTT GCTTGGGGAG GTGTTCGTGA ACGGCCAGGA GCTGCACAGG 
GAGCAGTTCC AGGACTGCTT CTCCTACGTC CTGCAGAGCG ACACTCTGCT GAGCAACCTC 
ACCGTCCGCG AGACGCTGAA CTACACGGCA CTCCTGGCCA TCCGCCGCGG TTCCCAGAGC 
TTCTTCCAGA AGAAGGTGGA GGCAGTCATG ACGGAGCTGA GTTTGAGCCA CGTGGCAGAT 
CAACTGATTG GCAACTACAG CTTTGGGGGA ATTTCCCATG GCGAGAGGCG CCGAGTCTCC 
ATTGCAGCCC AGCTCCTCCA GGATCCCAAG GTCATGCTGT TTGATGAGCC AACCACAGGT 
CTGGACTGCA TGACTGCAAA TCAGATCGTC GTCCTCCTAG CAGAGCTGGC TCGCAGGGAC 
CGCATTGTGA TCCTCACCAT CCACCAGCCC CGCTCTGAGC TCTTCCAGCT CTTTGACAAA 
ATCGCCATCC TGAGCTGCGG AGAGCTGGTT TTCTGTGGGA CACCGGTGGA AATGCTTGAT 
TTCTTCAGTG GCTGTGGTTA CCCTTGTCCT GAACATTCAA ACCCTTTTGA TTTTTATATG 
GACCTGACAT CAGTGGATAC CCAAAGCAAA GAACGAGAAA TAGAAACCTA CAGAAGAGTC 
CAGATGATTG AAGTTGCCTA CAGAGAATCA GCAATGTATC ACAAAACCAT GGAGGCTATT 
GAAAGAACAA AACATCTGAA AACATTACCA AAGATTCCTT TCAAAACCAG AGATTCTCCT 
GGAGCCCTCT CTAAACTGTG TGTTCTCTTG AGGAGAGTGA TGAGAAACTT ACTGAGAAAT 
AAGCTGGCGG TGACGATGCG TCTTACTCAG AATCTGATCA TGGGTCTATT CATCATTTTC 
TTCCTTCTAC GACTGGGGAA GGATGTGCTG AAGGGTGCCG TCCAGGATCG TGTGGGTCTC 
CTGTACCAGT TTGTGGGTGC CATGCCGTAC ACAGGCATGC TCAATGCTGT GACACTGTTT 
CCTGTGCTGC GTGCCGTCAG TGACCAGGAG AGCCAGGATG GCCTGTACCA CAAGTGGCAG 
ATGCTACTGG CCTACATGCT GCACGTGCTT CCCTTCAGCG TCACTGCCAC CCTCGTATGC 
TGCAGTGTGT GCTACTGGAC TCTGGCCTTA TACCCCGAGG CTGCCAGATT TGCATATTTC 
TCTGTTGCTC TCTTGGCCCC CCACTTAATT GGTGAATTTC TAAGCCTTGT GCTACTTGGC 
GTGATCCAAA ACCCAAATGT GGTCAACAGC GTGGTGGCCC TGCTCTCCAT TGCTGGGGTG 
CTTATGGGCT CTGGCTTCGT GAGAAACATA CAAGAAATGC CCATTCCTTT TAAAATCCTC 
GGTTATTTTA CATTCCAAAA ATATTGCTCT GAGATTCTCG TAGTCAATGA GTTCTATGGA 
CAGAATTTCA CTTGTGGCAA CTCAAACGCC TCTGTGTCAG CTAACCCAAT GTGTACCATC 
ACTCAAGGAG CTCAGTTCAT TGAGAGAAAC TGCCCAGGTG CAACATCCAG ATTCACAGCA 
AACTTTCTCA TTTTGTATTC ATTTATCCCA GCTCTTGTCA TCCTAGGAAT AATTGTGTTT 
AAAATAAGGG ATTATCTCAT TAGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019718.2
CDS	137..2086
Translation	MGELPFLTLGESSELQVNRSSQCSLEEVPAKPRHSLGIFHASYSVSHHVGPWWDIASRRQKWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLRRTGTLLGEVFVNGQELHREQFQDCFSYVLQSDTLLSNLTVRETLNYTALLAIRRGSQSFFQKKVEAVMTELSLSHVADQLIGNYSFGGISHGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLAELARRDRIVILTIHQPRSELFQLFDKIAILSCGELVFCGTPVEMLDFFSGCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETYRRVQMIEVAYRESAMYHKTMEAIERTKHLKTLPKIPFKTRDSPGALSKLCVLLRRVMRNLLRNKLAVTMRLTQNLIMGLFIIFFLLRLGKDVLKGAVQDRVGLLYQFVGAMPYTGMLNAVTLFPVLRAVSDQESQDGLYHKWQMLLAYMLHVLPFSVTATLVCCSVCYWTLALYPEAARFAYFSVALLAPHLIGEFLSLVLLGVIQNPNVVNSVVALLSIAGVLMGSGFVRNIQEMPIPFKILGYFTFQKYCSEILVVNEFYGQNFTCGNSNASVSANPMCTITQGAQFIERNCPGATSRFTANFLILYSFIPALVILGIIVFKIRDYLISR

Target ORF information:

RefSeq Version	NM_001024547.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP binding cassette subfamily G member 5 (ABCG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024547.2

ORF Insert Sequence:

ATGGGCGAAC TCCCCTTTTT GACTCTTGGG GAGTCCTCAG AACTCCAAGT AAACAGAAGC 
TCTCAGTGCT CCCTAGAGGA GGTTCCTGCA AAGCCTCGAC ACAGCCTGGG CATTTTCCAT 
GCCTCGTACA GTGTCAGCCA CCATGTGGGG CCCTGGTGGG ACATCGCATC TCGCCGACAG 
AAGTGGACCA GGCAGATCCT CAAAGATGTC TCCTTGTACG TCGAGAGCGG GCAGATCATG 
TGCATCTTAG GGAGCTCAGG CTCAGGGAAA ACCACGCTGC TGGACGCCAT GTCCGGGAGG 
CTGCGGCGCA CAGGGACCTT GCTTGGGGAG GTGTTCGTGA ACGGCCAGGA GCTGCACAGG 
GAGCAGTTCC AGGACTGCTT CTCCTACGTC CTGCAGAGCG ACACTCTGCT GAGCAACCTC 
ACCGTCCGCG AGACGCTGAA CTACACGGCA CTCCTGGCCA TCCGCCGCGG TTCCCAGAGC 
TTCTTCCAGA AGAAGGTGGA GGCAGTCATG ACGGAGCTGA GTTTGAGCCA CGTGGCAGAT 
CAACTGATTG GCAACTACAG CTTTGGGGGA ATTTCCCATG GCGAGAGGCG CCGAGTCTCC 
ATTGCAGCCC AGCTCCTCCA GGATCCCAAG GTCATGCTGT TTGATGAGCC AACCACAGGT 
CTGGACTGCA TGACTGCAAA TCAGATCGTC GTCCTCCTAG CAGAGCTGGC TCGCAGGGAC 
CGCATTGTGA TCCTCACCAT CCACCAGCCC CGCTCTGAGC TCTTCCAGCT CTTTGACAAA 
ATCGCCATCC TGAGCTGCGG AGAGCTGGTT TTCTGTGGGA CACCGGTGGA AATGCTTGAT 
TTCTTCAGTG GCTGTGGTTA CCCTTGTCCT GAACATTCAA ACCCTTTTGA TTTTTATATG 
GACCTGACAT CAGTGGATAC CCAAAGCAAA GAACGAGAAA TAGAAACCTA CAGAAGAGTC 
CAGATGATTG AAGTTGCCTA CAGAGAATCA GCAATGTATC ACAAAACCAT GGAGGCTATT 
GAAAGAACAA AACATCTGAA AACATTACCA AAGATTCCTT TCAAAACCAG AGATTCTCCT 
GGAGCCCTCT CTAAACTGTG TGTTCTCTTG AGGAGAGTGA TGAGAAACTT ACTGAGAAAT 
AAGCTGGCGG TGACGATGCG TCTTACTCAG AATCTGATCA TGGGTCTATT CATCATTTTC 
TTCCTTCTAC GACTGGGGAA GGATGTGCTG AAGGGTGCCG TCCAGGATCG TGTGGGTCTC 
CTGTACCAGT TTGTGGGTGC CATGCCGTAC ACAGGCATGC TCAATGCTGT GACACTGTTT 
CCTGTGCTGC GTGCCGTCAG TGACCAGGAG AGCCAGGATG GCCTGTACCA CAAGTGGCAG 
ATGCTACTGG CCTACATGCT GCACGTGCTT CCCTTCAGCG TCACTGCCAC CCTCGTATGC 
TGCAGTGTGT GCTACTGGAC TCTGGCCTTA TACCCCGAGG CTGCCAGATT TGCATATTTC 
TCTGTTGCTC TCTTGGCCCC CCACTTAATT GGTGAATTTC TAAGCCTTGT GCTACTTGGC 
GTGATCCAAA ACCCAAATGT GGTCAACAGC GTGGTGGCCC TGCTCTCCAT TGCTGGGGTG 
CTTATGGGCT CTGGCTTCGT GAGAAACATA CAAGAAATGC CCATTCCTTT TAAAATCCTC 
GGTTATTTTA CATTCCAAAA ATATTGCTCT GAGATTCTCG TAGTCAATGA GTTCTATGGA 
CAGAATTTCA CTTGTGGCAA CTCAAACGCC TCTGTGTCAG CTAACCCAAT GTGTACCATC 
ACTCAAGGAG CTCAGTTCAT TGAGAGAAAC TGCCCAGGTG CAACATCCAG ATTCACAGCA 
AACTTTCTCA TTTTGTATTC ATTTATCCCA GCTCTTGTCA TCCTAGGAAT AATTGTGTTT 
AAAATAAGGG ATTATCTCAT TAGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Identification, sequence analysis and mRNA tissue distribution of the bovine sterol transporters ABCG5 and ABCG8.
Journal of dairy science89(2)553-61(2006 Feb)
Viturro E,Farke C,Meyer HH,Albrecht C