MFN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MFN1
Entrez Gene ID	515180
Full Name	mitofusin 1
Synonyms	MFN2
General protein information	Preferred Names mitofusin 1 Names mitofusin-1 mitofusin 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206508.1	NP_001193437.1	mitofusin-1

Pathways from BioSystems

Homologies

	MFN1	XP_001108853.1
Pan troglodytes (chimpanzee)	MFN1	XP_516887.2
Canis lupus familiaris (dog)	MFN1	XP_545210.2
Bos taurus (cattle)	MFN1	NP_001193437.1
Rattus norvegicus (Norway rat)	Mfn1	NP_620432.1
Gallus gallus (chicken)	MFN1	NP_001012949.1
Danio rerio (zebrafish)	mfn1b	NP_956941.1
Homo sapiens (human)	MFN1	NP_284941.2
Mus musculus (house mouse)	Mfn1	NP_077162.2
Xenopus tropicalis (tropical clawed frog)	mfn1	NP_001016189.1

Gene Ontology
Bibliography

Related articles in PubMed

High expression of Mfn1 promotes early development of bovine SCNT embryos: improvement of mitochondrial membrane potential and oxidative metabolism.
Hua S, Zhang H, Song Y, Li R, Liu J, Wang Y, Quan F, Zhang Y
Mitochondrion12(2)320-7(2012 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results showed that high level of Mfn1 expression significantly improved the embryo development rates by increasing ATP level and Deltapsim, while reducing H(2)O(2) generation.
Title: High expression of Mfn1 promotes early development of bovine SCNT embryos: improvement of mitochondrial membrane potential and oxidative metabolism.

The following MFN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MFN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa40041	NM_001206508.1 Latest version!	Bos taurus mitofusin 1 (MFN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MFN1 ( NM_001206508.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193437.1 Bos taurus mitofusin 1 (MFN1), mRNA.

Price & Availability↑

CloneID	OBa40041
Clone ID Related Accession (Same CDS sequence)	NM_001206508.1
Accession Version	NM_001206508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	mitofusin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC123688.1. On or before Apr 28, 2011 this sequence version replaced XM_002702453.1, XM_002684903.1. ##Evidence-Data-START## Transcript exon combination :: BC123688.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGAAA CTGCTTCTCC ACTGAAGCAC TTCGTGCTGG CTAAGAAGAC AATTACAGCA 
ATCTTCGACC AGTTACTGGA GTTTGTTACT GAAGGATCAC ATTTTGTTGA AGCAACATAT 
AAGAATCCAG AGCTTGACCG AGTAGCTACG GAGGATGATC TGATAGAAAT ACAGAGGTAT 
AAAAACAAGC TTTCCGTCAT TGGTGAGGTG CTGTCTCGGA GACACATGAA AGTGGCCTTT 
TTTGGCAGGA CAAGCAGTGG GAAGAGCTCT GTTATCAATG CCATGTTGTG GGATAAAGTC 
CTCCCTAGTG GGATTGGCCA TACAACCAAT TGCTTCCTGA GTGTTGAAGG AACCGATGGA 
GATAAAGCCT ATCTCATGAC AGAAGGGTCA GATGAAAAAA GGAGTGTGAA GACAGTTAAT 
CAGCTGGCCC ACGCCCTTCA TATGGACAAA GACTTGAAAG CTGGGTGTCT TGTGCATGTG 
TTTTGGCCAA AGGCAAAATG TGCCCTCTTG AGAGATGACC TGGTTTTAGT AGACAGTCCG 
GGCACAGATG TCACTACAGA GCTGGATAGC TGGATTGATA AGTTTTGCTT AGATGCTGAT 
GTCTTCGTTT TGGTTGCAAA TTCCGAATCA ACTCTAATGA ACACGGAAAA ACAGTTTTTT 
CACAAGGTAA ATGAGCGACT TTCCAAGCCT AATATTTTTA TCCTGAATAA TCGTTGGGAT 
GCCTCTGCAT CAGAGCCAGA ATATATGGAA GATGTACGCA GACAGCACAT GGAAAGATGC 
CTGCATTTTT TGGTGGAGGA GCTCAGAGTT GTGGATCCTT TAGAAGCACG CAATCGTATC 
TTTTTTGTTT CTGCAAAGGA AGTTCTTAGT GCTAGAAAGC ACAAAGCACA GGGGATGCCA 
GAAGGTGGTG GGGCACTTGC AGAAGGATTT CAGGCAAGAT TACAAGAGTT TCAGAATTTT 
GAACAAATCT TTGAGGAGTG TATCTCGCAG TCAGCAGTGA AAACAAAGTT TGAACAGCAC 
ACTATCAGAG CTAAACAGAT ACTAGAAACT GTGAAAAACA TAATGGATTC AATAAACGTG 
GCAGCAGCAG AGAAAAGGGT TTATTCAATA GAAGAGAGGG AAGACCAAAT TGATAGACTG 
GACTTTATCC GAAACCAGAT GAACCTTTTA ACACTGGATG TTAAAAAAAA AATCAGGGAG 
GTTACAGAGG AGGTTGCAAA TAAGGTTTCA TGTGCAATGA CAGATGAAAT CTGTCGACTC 
TCTGTTTTGG TTGATGAATT TTGTTCTGAG TTTCATCCTA CTCCAAGTGT ACTGAAAGTA 
TATAAAAATG AGTTAAATAA GCACATAGAA GACGGAATGG GAAGAAATTT GGCTGATCGG 
TGCACCAATG AAGTCAACGC TTCAATGCTT CAATCCCAGC AAGAAATTAT TGAAAATTTG 
AAGCCATTAC TTCCAGCTGG TATTCAGAAT AAACTACACA CACTGGTTCC TTGCAAGAAA 
TTTGATCTTA GCTATGATCT AAATTGCCAC AAGTTATGTT CAGATTTTCA AGAGGATATT 
GTATTTCGTT TTTCTCTGGG CTGGGCTTCT CTTGTCCATC GTTTCTTGGG CCCTACAAAT 
GCTCAGCGGG TGCTTCTAGG ATTATCGGAG CCTATCTTTC AGCTCCCCAG ATCTTTAGCT 
TCTACTCCCA CTGCTCCTTC CAACCCAGCA ACACCAGATA ATGCATCACA GGAAGAACTC 
ATGGTTACCC TAATAACAAC ATTAGCCTCT CTTACATCTA GAACGTCTAT GGGCATCATT 
GTTGTTGGAG GAGTGATTTG GAAAACTGTA GGTTGGAAAC TCATATCTGT TTCATTAAGT 
ATGTATGGAG CTTTGTATCT TTATGAAAGG CTCACTTGGA CCACCCGTGC CAAGGAGAGA 
GCCTTTAAAC AGCAATTTGT GAATTATGCA ACTGAAAAAC TGCAGATGAT TGTTAGCTTC 
ACAAGCGCAA ACTGCAGCCA TCAAGTACAA CAGGAAATGG CAACCACTTT TGCTCGCCTG 
TGCCAACAAG TTGATATTAC ACAAAGACAT CTGGAAGAAG AAATTGCTAG ATTATCCAAA 
GAAATAGATC AATTGGAGAA AATACAAAAC AATTCAAAGC TCTTAAGAAA TAAAGCTGTT 
CAACTTGAAA ACCAGCTGGA GAATTTTACT AAGCGGTTTC TACATTCAAG CAGAGAAGAA 
GAATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193437.1
CDS	69..2297
Translation	MAETASPLKHFVLAKKTITAIFDQLLEFVTEGSHFVEATYKNPELDRVATEDDLIEIQRYKNKLSVIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKRSVKTVNQLAHALHMDKDLKAGCLVHVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKQFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELRVVDPLEARNRIFFVSAKEVLSARKHKAQGMPEGGGALAEGFQARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILETVKNIMDSINVAAAEKRVYSIEEREDQIDRLDFIRNQMNLLTLDVKKKIREVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPTPSVLKVYKNELNKHIEDGMGRNLADRCTNEVNASMLQSQQEIIENLKPLLPAGIQNKLHTLVPCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWASLVHRFLGPTNAQRVLLGLSEPIFQLPRSLASTPTAPSNPATPDNASQEELMVTLITTLASLTSRTSMGIIVVGGVIWKTVGWKLISVSLSMYGALYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVSFTSANCSHQVQQEMATTFARLCQQVDITQRHLEEEIARLSKEIDQLEKIQNNSKLLRNKAVQLENQLENFTKRFLHSSREEES

Target ORF information:

RefSeq Version	NM_001206508.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mitofusin 1 (MFN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206508.1

ORF Insert Sequence:

ATGGCAGAAA CTGCTTCTCC ACTGAAGCAC TTCGTGCTGG CTAAGAAGAC AATTACAGCA 
ATCTTCGACC AGTTACTGGA GTTTGTTACT GAAGGATCAC ATTTTGTTGA AGCAACATAT 
AAGAATCCAG AGCTTGACCG AGTAGCTACG GAGGATGATC TGATAGAAAT ACAGAGGTAT 
AAAAACAAGC TTTCCGTCAT TGGTGAGGTG CTGTCTCGGA GACACATGAA AGTGGCCTTT 
TTTGGCAGGA CAAGCAGTGG GAAGAGCTCT GTTATCAATG CCATGTTGTG GGATAAAGTC 
CTCCCTAGTG GGATTGGCCA TACAACCAAT TGCTTCCTGA GTGTTGAAGG AACCGATGGA 
GATAAAGCCT ATCTCATGAC AGAAGGGTCA GATGAAAAAA GGAGTGTGAA GACAGTTAAT 
CAGCTGGCCC ACGCCCTTCA TATGGACAAA GACTTGAAAG CTGGGTGTCT TGTGCATGTG 
TTTTGGCCAA AGGCAAAATG TGCCCTCTTG AGAGATGACC TGGTTTTAGT AGACAGTCCG 
GGCACAGATG TCACTACAGA GCTGGATAGC TGGATTGATA AGTTTTGCTT AGATGCTGAT 
GTCTTCGTTT TGGTTGCAAA TTCCGAATCA ACTCTAATGA ACACGGAAAA ACAGTTTTTT 
CACAAGGTAA ATGAGCGACT TTCCAAGCCT AATATTTTTA TCCTGAATAA TCGTTGGGAT 
GCCTCTGCAT CAGAGCCAGA ATATATGGAA GATGTACGCA GACAGCACAT GGAAAGATGC 
CTGCATTTTT TGGTGGAGGA GCTCAGAGTT GTGGATCCTT TAGAAGCACG CAATCGTATC 
TTTTTTGTTT CTGCAAAGGA AGTTCTTAGT GCTAGAAAGC ACAAAGCACA GGGGATGCCA 
GAAGGTGGTG GGGCACTTGC AGAAGGATTT CAGGCAAGAT TACAAGAGTT TCAGAATTTT 
GAACAAATCT TTGAGGAGTG TATCTCGCAG TCAGCAGTGA AAACAAAGTT TGAACAGCAC 
ACTATCAGAG CTAAACAGAT ACTAGAAACT GTGAAAAACA TAATGGATTC AATAAACGTG 
GCAGCAGCAG AGAAAAGGGT TTATTCAATA GAAGAGAGGG AAGACCAAAT TGATAGACTG 
GACTTTATCC GAAACCAGAT GAACCTTTTA ACACTGGATG TTAAAAAAAA AATCAGGGAG 
GTTACAGAGG AGGTTGCAAA TAAGGTTTCA TGTGCAATGA CAGATGAAAT CTGTCGACTC 
TCTGTTTTGG TTGATGAATT TTGTTCTGAG TTTCATCCTA CTCCAAGTGT ACTGAAAGTA 
TATAAAAATG AGTTAAATAA GCACATAGAA GACGGAATGG GAAGAAATTT GGCTGATCGG 
TGCACCAATG AAGTCAACGC TTCAATGCTT CAATCCCAGC AAGAAATTAT TGAAAATTTG 
AAGCCATTAC TTCCAGCTGG TATTCAGAAT AAACTACACA CACTGGTTCC TTGCAAGAAA 
TTTGATCTTA GCTATGATCT AAATTGCCAC AAGTTATGTT CAGATTTTCA AGAGGATATT 
GTATTTCGTT TTTCTCTGGG CTGGGCTTCT CTTGTCCATC GTTTCTTGGG CCCTACAAAT 
GCTCAGCGGG TGCTTCTAGG ATTATCGGAG CCTATCTTTC AGCTCCCCAG ATCTTTAGCT 
TCTACTCCCA CTGCTCCTTC CAACCCAGCA ACACCAGATA ATGCATCACA GGAAGAACTC 
ATGGTTACCC TAATAACAAC ATTAGCCTCT CTTACATCTA GAACGTCTAT GGGCATCATT 
GTTGTTGGAG GAGTGATTTG GAAAACTGTA GGTTGGAAAC TCATATCTGT TTCATTAAGT 
ATGTATGGAG CTTTGTATCT TTATGAAAGG CTCACTTGGA CCACCCGTGC CAAGGAGAGA 
GCCTTTAAAC AGCAATTTGT GAATTATGCA ACTGAAAAAC TGCAGATGAT TGTTAGCTTC 
ACAAGCGCAA ACTGCAGCCA TCAAGTACAA CAGGAAATGG CAACCACTTT TGCTCGCCTG 
TGCCAACAAG TTGATATTAC ACAAAGACAT CTGGAAGAAG AAATTGCTAG ATTATCCAAA 
GAAATAGATC AATTGGAGAA AATACAAAAC AATTCAAAGC TCTTAAGAAA TAAAGCTGTT 
CAACTTGAAA ACCAGCTGGA GAATTTTACT AAGCGGTTTC TACATTCAAG CAGAGAAGAA 
GAATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High expression of Mfn1 promotes early development of bovine SCNT embryos: improvement of mitochondrial membrane potential and oxidative metabolism.
Mitochondrion12(2)320-7(2012 Mar)
Hua S,Zhang H,Song Y,Li R,Liu J,Wang Y,Quan F,Zhang Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MFN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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