GIT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GIT1
Entrez Gene ID	515088
Full Name	GIT ArfGAP 1
General protein information	Preferred Names GIT ArfGAP 1 Names ARF GTPase-activating protein GIT1 G protein-coupled receptor kinase interactor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002695704.5	XP_002695750.3	ARF GTPase-activating protein GIT1 isoform X1
XM_015467970.1	XP_015323456.1	ARF GTPase-activating protein GIT1 isoform X3
XM_005220138.3	XP_005220195.1	ARF GTPase-activating protein GIT1 isoform X2
XM_024980691.1	XP_024836459.1	ARF GTPase-activating protein GIT1 isoform X1
XM_024980693.1	XP_024836461.1	ARF GTPase-activating protein GIT1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC568935	XP_697386.4
Mus musculus (house mouse)	Git1	NP_001004144.1
Gallus gallus (chicken)	GIT1	NP_989627.1
Homo sapiens (human)	GIT1	NP_001078923.1
	GIT1	XP_001108023.2
Rattus norvegicus (Norway rat)	Git1	NP_114002.1
Pan troglodytes (chimpanzee)	GIT1	XP_511377.3
Canis lupus familiaris (dog)	GIT1	XP_005625057.1
Bos taurus (cattle)	GIT1	XP_002695750.3

Gene Ontology

The following GIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa10019	XM_002695704.5 Latest version!	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa10020	XM_005220138.3 Latest version!	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa226717	XM_024980691.1 Latest version!	Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa226718	XM_024980693.1 Latest version!	Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa95029	XM_015467970.1 Latest version!	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIT1 ( XM_002695704.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695750.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10019
Clone ID Related Accession (Same CDS sequence)	XM_002695704.5
Accession Version	XM_002695704.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695704.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGGA GGCTCAGCCG TGTCTGGCTG TGGCTGACCC TTGTCCCTGC TCACCCCGCA 
GACCCCGGCT GGGCCTCCAT CAGCAGGGGT GTGCTGGTGT GTGACGAGTG CTGCAGCGTG 
CACCGGAGTC TGGGCCGCCA CATCTCCATT GTCAAGCACC TCCGCCACAG CGCCTGGCCC 
CCCACCCTGC TGCAGATGGT GCACACGCTT GCCAGCAACG GGGCCAACTC CATCTGGGAG 
CACTCCCTGC TGGACCCCGC GCAGGTGCAG AGCGGCCGGC GTAAAGCCAA CCCCCAAGAC 
AAAGTCCACC CCATCAAGTC CGAGTTCATC AGGGCCAAGT ACCAGATGCT GGCTTTTGTG 
CACAAGCTGC CCTGCCGGGA CGATGACGGA GTCACCGCCA AAGACCTCAG CAAGCAACTG 
CACTCAAGCG TGCGAACGGG CAACCTGGAG ACATGTCTGC GCCTGCTATC CCTGGGCGCC 
CAGGCCAACT TCTTCCACCC CGAAAAGGGC ACCACACCTC TGCATGTGGC TGCCAAGGCA 
GGTCAGACGC TGCAGGCCGA GCTGCTTGTG GTGTATGGGG CTGACCCTGG CTCCCCAGAT 
GTTAACGGCC GTACACCCAT TGATTATGCC AGGCAGGCGG GGCACCATGA GCTGGCGGAA 
CGGCTAGTCG AGTGCCAGTA CGAGCTCACT GACCGGTTGG CCTTCTATCT CTGTGGGCGC 
AAGCCGGATC ACAAGAATGG GCATTACATC ATCCCACAGA TGGCAGACAG ATCTCGGCAA 
AAGTGCATGT CTCAGAGCCT GGACCTGTCC GAGTTGGCCA AAGCTGCCAA GAAGAAGCTG 
CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTAGCCATGG ACGTGTATGA CGAGGTGGAT 
CGAAGGGAAA ATGACGCTGT GTGGCTGGCG ACCCAGAACC ACAGCACCCT GGTGACCGAG 
CGCAGCGCCG TGCCCTTCCT GCCAGTGAAC CCCGAGTACT CAGCCACACG GAATCAGGGG 
CGACAGAAGC TGGCCCGCTT TAACGCCCGG GAGTTTGCCA CCTTGATCAT CGACATCCTC 
AGCGAGGCCA AGAGGAGACA GCAGGGCAAG AGCCTGGGCA GCCCCACAGA CAACCTCGAG 
CTGTCCACGC GGGGCCAGAG TGACCTGGAC GACCAGCACG ACTACGACAG CGTGGCCTCG 
GACGAGGACA CAGACCAGGA GCCCCTGCGC AGTGCTGGTG CCACGCGGAA CAACAGAGCT 
CGGAGCATGG ACTCCTCAGA CCTGTCAGAC GGGGCTGTGA CGCTGCAAGA GTACCTGGAG 
CTGAAAAAGG CCCTGGCTGC TTCCGAGGCC AAGGTGCAGC AGCTCATGAA GGTCAATAGC 
AGCCTGAGTG ATGAGCTCCG GAGGCTGCAG AGGGAGATCC ACAAGCTGCA GGCCGAGAAC 
CTGCAGATCC GGCAGCCACC AGGGCCGATG CCCACACCCC CCCTCCCCAG CGAGCGAGCT 
GAGCACGCAC CCGTGGGGCC TGGCGGGAGC ACCCACCGCA GGGACCGCCA GGCCTTTTCC 
ATGTATGAAC CAGGCTCTGC CCTGAAGCCC TTTGGGGTCC CGCCCGGGGA CGAGCTCACC 
ACCCGGCTCC AGCCTTTCCA CAGCACCGAG CTGGAGGACG ACGCCATCTA TTCAGTGCAC 
GTCCCTGCTG GCCTTTACCG GATCCGGAAG GGGGTGTCGG CCTCAGCTGT GCCCTTCACT 
CCCTCCTCCC CACTGCTGTC GTGCTCCCAG GAAGGAAGCC GCCACACGAG CAAGCTCTCC 
CGCCACGGCA GCGGCGCAGA CAGTGACTAT GAGAACACAC AAAGTGGGGA CCCGCTGCTG 
GGCCTGGAAG GGAAGAGGTT TCTGGAGCTG GGCAAGGAAG AGGACTTCCA CCCGGAGCTG 
GAAAGCCTGG ATGGCGACCT CGACCCTGGG CTTCCCAGCA CGGAGGATGT CATCCTGAAG 
ACGGAGCAGG TCACCAAGAA CATTCAGGAG TTACTGCGGG CGGCCCAGGA ATTCAAGCAT 
GACAGCTTCG TGCCCTGCTC AGAGAAGATC CACTTGGCTG TGACTGAGAT GGCCTCTCTC 
TTCCCGAAGA GGCCCGCCCT GGAGCCTGTG CGAAGCTCGC TGCGGCTGCT CAACGCCAGC 
GCCTACCGGC TGCAGAGCGA GTGCCGGAAG ACGGTGCCCC CAGAGCCTGG CGCTCCCGTG 
GACTTCCAGC TGCTGACTCA GCAGGTGATC CAGTGCGCCT ACGACATCGC CAAGGCCGCC 
AAGCAGCTGG TCACCATCAC CACCCGCGAG AAGAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695750.3
CDS	2355..4676
Translation	MLGRLSRVWLWLTLVPAHPADPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLGSPTDNLELSTRGQSDLDDQHDYDSVASDEDTDQEPLRSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALAASEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQIRQPPGPMPTPPLPSERAEHAPVGPGGSTHRRDRQAFSMYEPGSALKPFGVPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_002695704.5
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695704.5

ORF Insert Sequence:

ATGCTCGGGA GGCTCAGCCG TGTCTGGCTG TGGCTGACCC TTGTCCCTGC TCACCCCGCA 
GACCCCGGCT GGGCCTCCAT CAGCAGGGGT GTGCTGGTGT GTGACGAGTG CTGCAGCGTG 
CACCGGAGTC TGGGCCGCCA CATCTCCATT GTCAAGCACC TCCGCCACAG CGCCTGGCCC 
CCCACCCTGC TGCAGATGGT GCACACGCTT GCCAGCAACG GGGCCAACTC CATCTGGGAG 
CACTCCCTGC TGGACCCCGC GCAGGTGCAG AGCGGCCGGC GTAAAGCCAA CCCCCAAGAC 
AAAGTCCACC CCATCAAGTC CGAGTTCATC AGGGCCAAGT ACCAGATGCT GGCTTTTGTG 
CACAAGCTGC CCTGCCGGGA CGATGACGGA GTCACCGCCA AAGACCTCAG CAAGCAACTG 
CACTCAAGCG TGCGAACGGG CAACCTGGAG ACATGTCTGC GCCTGCTATC CCTGGGCGCC 
CAGGCCAACT TCTTCCACCC CGAAAAGGGC ACCACACCTC TGCATGTGGC TGCCAAGGCA 
GGTCAGACGC TGCAGGCCGA GCTGCTTGTG GTGTATGGGG CTGACCCTGG CTCCCCAGAT 
GTTAACGGCC GTACACCCAT TGATTATGCC AGGCAGGCGG GGCACCATGA GCTGGCGGAA 
CGGCTAGTCG AGTGCCAGTA CGAGCTCACT GACCGGTTGG CCTTCTATCT CTGTGGGCGC 
AAGCCGGATC ACAAGAATGG GCATTACATC ATCCCACAGA TGGCAGACAG ATCTCGGCAA 
AAGTGCATGT CTCAGAGCCT GGACCTGTCC GAGTTGGCCA AAGCTGCCAA GAAGAAGCTG 
CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTAGCCATGG ACGTGTATGA CGAGGTGGAT 
CGAAGGGAAA ATGACGCTGT GTGGCTGGCG ACCCAGAACC ACAGCACCCT GGTGACCGAG 
CGCAGCGCCG TGCCCTTCCT GCCAGTGAAC CCCGAGTACT CAGCCACACG GAATCAGGGG 
CGACAGAAGC TGGCCCGCTT TAACGCCCGG GAGTTTGCCA CCTTGATCAT CGACATCCTC 
AGCGAGGCCA AGAGGAGACA GCAGGGCAAG AGCCTGGGCA GCCCCACAGA CAACCTCGAG 
CTGTCCACGC GGGGCCAGAG TGACCTGGAC GACCAGCACG ACTACGACAG CGTGGCCTCG 
GACGAGGACA CAGACCAGGA GCCCCTGCGC AGTGCTGGTG CCACGCGGAA CAACAGAGCT 
CGGAGCATGG ACTCCTCAGA CCTGTCAGAC GGGGCTGTGA CGCTGCAAGA GTACCTGGAG 
CTGAAAAAGG CCCTGGCTGC TTCCGAGGCC AAGGTGCAGC AGCTCATGAA GGTCAATAGC 
AGCCTGAGTG ATGAGCTCCG GAGGCTGCAG AGGGAGATCC ACAAGCTGCA GGCCGAGAAC 
CTGCAGATCC GGCAGCCACC AGGGCCGATG CCCACACCCC CCCTCCCCAG CGAGCGAGCT 
GAGCACGCAC CCGTGGGGCC TGGCGGGAGC ACCCACCGCA GGGACCGCCA GGCCTTTTCC 
ATGTATGAAC CAGGCTCTGC CCTGAAGCCC TTTGGGGTCC CGCCCGGGGA CGAGCTCACC 
ACCCGGCTCC AGCCTTTCCA CAGCACCGAG CTGGAGGACG ACGCCATCTA TTCAGTGCAC 
GTCCCTGCTG GCCTTTACCG GATCCGGAAG GGGGTGTCGG CCTCAGCTGT GCCCTTCACT 
CCCTCCTCCC CACTGCTGTC GTGCTCCCAG GAAGGAAGCC GCCACACGAG CAAGCTCTCC 
CGCCACGGCA GCGGCGCAGA CAGTGACTAT GAGAACACAC AAAGTGGGGA CCCGCTGCTG 
GGCCTGGAAG GGAAGAGGTT TCTGGAGCTG GGCAAGGAAG AGGACTTCCA CCCGGAGCTG 
GAAAGCCTGG ATGGCGACCT CGACCCTGGG CTTCCCAGCA CGGAGGATGT CATCCTGAAG 
ACGGAGCAGG TCACCAAGAA CATTCAGGAG TTACTGCGGG CGGCCCAGGA ATTCAAGCAT 
GACAGCTTCG TGCCCTGCTC AGAGAAGATC CACTTGGCTG TGACTGAGAT GGCCTCTCTC 
TTCCCGAAGA GGCCCGCCCT GGAGCCTGTG CGAAGCTCGC TGCGGCTGCT CAACGCCAGC 
GCCTACCGGC TGCAGAGCGA GTGCCGGAAG ACGGTGCCCC CAGAGCCTGG CGCTCCCGTG 
GACTTCCAGC TGCTGACTCA GCAGGTGATC CAGTGCGCCT ACGACATCGC CAAGGCCGCC 
AAGCAGCTGG TCACCATCAC CACCCGCGAG AAGAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_005220138.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220195.1 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10020
Clone ID Related Accession (Same CDS sequence)	XM_005220138.3
Accession Version	XM_005220138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGGA GGCTCAGCCG TGTCTGGCTG TGGCTGACCC TTGTCCCTGC TCACCCCGCA 
GACCCCGGCT GGGCCTCCAT CAGCAGGGGT GTGCTGGTGT GTGACGAGTG CTGCAGCGTG 
CACCGGAGTC TGGGCCGCCA CATCTCCATT GTCAAGCACC TCCGCCACAG CGCCTGGCCC 
CCCACCCTGC TGCAGATGGT GCACACGCTT GCCAGCAACG GGGCCAACTC CATCTGGGAG 
CACTCCCTGC TGGACCCCGC GCAGGTGCAG AGCGGCCGGC GTAAAGCCAA CCCCCAAGAC 
AAAGTCCACC CCATCAAGTC CGAGTTCATC AGGGCCAAGT ACCAGATGCT GGCTTTTGTG 
CACAAGCTGC CCTGCCGGGA CGATGACGGA GTCACCGCCA AAGACCTCAG CAAGCAACTG 
CACTCAAGCG TGCGAACGGG CAACCTGGAG ACATGTCTGC GCCTGCTATC CCTGGGCGCC 
CAGGCCAACT TCTTCCACCC CGAAAAGGGC ACCACACCTC TGCATGTGGC TGCCAAGGCA 
GGTCAGACGC TGCAGGCCGA GCTGCTTGTG GTGTATGGGG CTGACCCTGG CTCCCCAGAT 
GTTAACGGCC GTACACCCAT TGATTATGCC AGGCAGGCGG GGCACCATGA GCTGGCGGAA 
CGGCTAGTCG AGTGCCAGTA CGAGCTCACT GACCGGTTGG CCTTCTATCT CTGTGGGCGC 
AAGCCGGATC ACAAGAATGG GCATTACATC ATCCCACAGA TGGCAGACAG CCTGGACCTG 
TCCGAGTTGG CCAAAGCTGC CAAGAAGAAG CTGCAGGCGC TCAGCAACCG GCTTTTTGAG 
GAACTAGCCA TGGACGTGTA TGACGAGGTG GATCGAAGGG AAAATGACGC TGTGTGGCTG 
GCGACCCAGA ACCACAGCAC CCTGGTGACC GAGCGCAGCG CCGTGCCCTT CCTGCCAGTG 
AACCCCGAGT ACTCAGCCAC ACGGAATCAG GGGCGACAGA AGCTGGCCCG CTTTAACGCC 
CGGGAGTTTG CCACCTTGAT CATCGACATC CTCAGCGAGG CCAAGAGGAG ACAGCAGGGC 
AAGAGCCTGG GCAGCCCCAC AGACAACCTC GAGCTGTCCA CGCGGGGCCA GAGTGACCTG 
GACGACCAGC ACGACTACGA CAGCGTGGCC TCGGACGAGG ACACAGACCA GGAGCCCCTG 
CGCAGTGCTG GTGCCACGCG GAACAACAGA GCTCGGAGCA TGGACTCCTC AGACCTGTCA 
GACGGGGCTG TGACGCTGCA AGAGTACCTG GAGCTGAAAA AGGCCCTGGC TGCTTCCGAG 
GCCAAGGTGC AGCAGCTCAT GAAGGTCAAT AGCAGCCTGA GTGATGAGCT CCGGAGGCTG 
CAGAGGGAGA TCCACAAGCT GCAGGCCGAG AACCTGCAGA TCCGGCAGCC ACCAGGGCCG 
ATGCCCACAC CCCCCCTCCC CAGCGAGCGA GCTGAGCACG CACCCGTGGG GCCTGGCGGG 
AGCACCCACC GCAGGGACCG CCAGGCCTTT TCCATGTATG AACCAGGCTC TGCCCTGAAG 
CCCTTTGGGG TCCCGCCCGG GGACGAGCTC ACCACCCGGC TCCAGCCTTT CCACAGCACC 
GAGCTGGAGG ACGACGCCAT CTATTCAGTG CACGTCCCTG CTGGCCTTTA CCGGATCCGG 
AAGGGGGTGT CGGCCTCAGC TGTGCCCTTC ACTCCCTCCT CCCCACTGCT GTCGTGCTCC 
CAGGAAGGAA GCCGCCACAC GAGCAAGCTC TCCCGCCACG GCAGCGGCGC AGACAGTGAC 
TATGAGAACA CACAAAGTGG GGACCCGCTG CTGGGCCTGG AAGGGAAGAG GTTTCTGGAG 
CTGGGCAAGG AAGAGGACTT CCACCCGGAG CTGGAAAGCC TGGATGGCGA CCTCGACCCT 
GGGCTTCCCA GCACGGAGGA TGTCATCCTG AAGACGGAGC AGGTCACCAA GAACATTCAG 
GAGTTACTGC GGGCGGCCCA GGAATTCAAG CATGACAGCT TCGTGCCCTG CTCAGAGAAG 
ATCCACTTGG CTGTGACTGA GATGGCCTCT CTCTTCCCGA AGAGGCCCGC CCTGGAGCCT 
GTGCGAAGCT CGCTGCGGCT GCTCAACGCC AGCGCCTACC GGCTGCAGAG CGAGTGCCGG 
AAGACGGTGC CCCCAGAGCC TGGCGCTCCC GTGGACTTCC AGCTGCTGAC TCAGCAGGTG 
ATCCAGTGCG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCCGC 
GAGAAGAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220195.1
CDS	2354..4648
Translation	MLGRLSRVWLWLTLVPAHPADPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLGSPTDNLELSTRGQSDLDDQHDYDSVASDEDTDQEPLRSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALAASEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQIRQPPGPMPTPPLPSERAEHAPVGPGGSTHRRDRQAFSMYEPGSALKPFGVPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_005220138.3
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220138.3

ORF Insert Sequence:

ATGCTCGGGA GGCTCAGCCG TGTCTGGCTG TGGCTGACCC TTGTCCCTGC TCACCCCGCA 
GACCCCGGCT GGGCCTCCAT CAGCAGGGGT GTGCTGGTGT GTGACGAGTG CTGCAGCGTG 
CACCGGAGTC TGGGCCGCCA CATCTCCATT GTCAAGCACC TCCGCCACAG CGCCTGGCCC 
CCCACCCTGC TGCAGATGGT GCACACGCTT GCCAGCAACG GGGCCAACTC CATCTGGGAG 
CACTCCCTGC TGGACCCCGC GCAGGTGCAG AGCGGCCGGC GTAAAGCCAA CCCCCAAGAC 
AAAGTCCACC CCATCAAGTC CGAGTTCATC AGGGCCAAGT ACCAGATGCT GGCTTTTGTG 
CACAAGCTGC CCTGCCGGGA CGATGACGGA GTCACCGCCA AAGACCTCAG CAAGCAACTG 
CACTCAAGCG TGCGAACGGG CAACCTGGAG ACATGTCTGC GCCTGCTATC CCTGGGCGCC 
CAGGCCAACT TCTTCCACCC CGAAAAGGGC ACCACACCTC TGCATGTGGC TGCCAAGGCA 
GGTCAGACGC TGCAGGCCGA GCTGCTTGTG GTGTATGGGG CTGACCCTGG CTCCCCAGAT 
GTTAACGGCC GTACACCCAT TGATTATGCC AGGCAGGCGG GGCACCATGA GCTGGCGGAA 
CGGCTAGTCG AGTGCCAGTA CGAGCTCACT GACCGGTTGG CCTTCTATCT CTGTGGGCGC 
AAGCCGGATC ACAAGAATGG GCATTACATC ATCCCACAGA TGGCAGACAG CCTGGACCTG 
TCCGAGTTGG CCAAAGCTGC CAAGAAGAAG CTGCAGGCGC TCAGCAACCG GCTTTTTGAG 
GAACTAGCCA TGGACGTGTA TGACGAGGTG GATCGAAGGG AAAATGACGC TGTGTGGCTG 
GCGACCCAGA ACCACAGCAC CCTGGTGACC GAGCGCAGCG CCGTGCCCTT CCTGCCAGTG 
AACCCCGAGT ACTCAGCCAC ACGGAATCAG GGGCGACAGA AGCTGGCCCG CTTTAACGCC 
CGGGAGTTTG CCACCTTGAT CATCGACATC CTCAGCGAGG CCAAGAGGAG ACAGCAGGGC 
AAGAGCCTGG GCAGCCCCAC AGACAACCTC GAGCTGTCCA CGCGGGGCCA GAGTGACCTG 
GACGACCAGC ACGACTACGA CAGCGTGGCC TCGGACGAGG ACACAGACCA GGAGCCCCTG 
CGCAGTGCTG GTGCCACGCG GAACAACAGA GCTCGGAGCA TGGACTCCTC AGACCTGTCA 
GACGGGGCTG TGACGCTGCA AGAGTACCTG GAGCTGAAAA AGGCCCTGGC TGCTTCCGAG 
GCCAAGGTGC AGCAGCTCAT GAAGGTCAAT AGCAGCCTGA GTGATGAGCT CCGGAGGCTG 
CAGAGGGAGA TCCACAAGCT GCAGGCCGAG AACCTGCAGA TCCGGCAGCC ACCAGGGCCG 
ATGCCCACAC CCCCCCTCCC CAGCGAGCGA GCTGAGCACG CACCCGTGGG GCCTGGCGGG 
AGCACCCACC GCAGGGACCG CCAGGCCTTT TCCATGTATG AACCAGGCTC TGCCCTGAAG 
CCCTTTGGGG TCCCGCCCGG GGACGAGCTC ACCACCCGGC TCCAGCCTTT CCACAGCACC 
GAGCTGGAGG ACGACGCCAT CTATTCAGTG CACGTCCCTG CTGGCCTTTA CCGGATCCGG 
AAGGGGGTGT CGGCCTCAGC TGTGCCCTTC ACTCCCTCCT CCCCACTGCT GTCGTGCTCC 
CAGGAAGGAA GCCGCCACAC GAGCAAGCTC TCCCGCCACG GCAGCGGCGC AGACAGTGAC 
TATGAGAACA CACAAAGTGG GGACCCGCTG CTGGGCCTGG AAGGGAAGAG GTTTCTGGAG 
CTGGGCAAGG AAGAGGACTT CCACCCGGAG CTGGAAAGCC TGGATGGCGA CCTCGACCCT 
GGGCTTCCCA GCACGGAGGA TGTCATCCTG AAGACGGAGC AGGTCACCAA GAACATTCAG 
GAGTTACTGC GGGCGGCCCA GGAATTCAAG CATGACAGCT TCGTGCCCTG CTCAGAGAAG 
ATCCACTTGG CTGTGACTGA GATGGCCTCT CTCTTCCCGA AGAGGCCCGC CCTGGAGCCT 
GTGCGAAGCT CGCTGCGGCT GCTCAACGCC AGCGCCTACC GGCTGCAGAG CGAGTGCCGG 
AAGACGGTGC CCCCAGAGCC TGGCGCTCCC GTGGACTTCC AGCTGCTGAC TCAGCAGGTG 
ATCCAGTGCG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCCGC 
GAGAAGAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_024980691.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836459.1 Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226717
Clone ID Related Accession (Same CDS sequence)	XM_024980691.1
Accession Version	XM_024980691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCCGGC 
TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT 
CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG 
CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CCGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG 
CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC 
GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTAT CCCTGGGCGC CCAGGCCAAC 
TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG 
CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC 
CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA ACGGCTAGTC 
GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT 
CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GCCTGGACCT GTCCGAGTTG 
GCCAAAGCTG CCAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTAGCC 
ATGGACGTGT ATGACGAGGT GGATCGAAGG GAAAATGACG CTGTGTGGCT GGCGACCCAG 
AACCACAGCA CCCTGGTGAC CGAGCGCAGC GCCGTGCCCT TCCTGCCAGT GAACCCCGAG 
TACTCAGCCA CACGGAATCA GGGGCGACAG AAGCTGGCCC GCTTTAACGC CCGGGAGTTT 
GCCACCTTGA TCATCGACAT CCTCAGCGAG GCCAAGAGGA GACAGCAGGG CAAGAGCCTG 
GGCAGCCCCA CAGACAACCT CGAGCTGTCC ACGCGGGGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTGGC CTCGGACGAG GACACAGACC AGGAGCCCCT GCGCAGTGCT 
GGTGCCACGC GGAACAACAG AGCTCGGAGC ATGGACTCCT CAGACCTGTC AGACGGGGCT 
GTGACGCTGC AAGAGTACCT GGAGCTGAAA AAGGCCCTGG CTGCTTCCGA GGCCAAGGTG 
CAGCAGCTCA TGAAGGTCAA TAGCAGCCTG AGTGATGAGC TCCGGAGGCT GCAGAGGGAG 
ATCCACAAGC TGCAGGCCGA GAACCTGCAG ATCCGGCAGC CACCAGGGCC GATGCCCACA 
CCCCCCCTCC CCAGCGAGCG AGCTGAGCAC GCACCCGTGG GGCCTGGCGG GAGCACCCAC 
CGCAGGGACC GCCAGGCCTT TTCCATGTAT GAACCAGGCT CTGCCCTGAA GCCCTTTGGG 
GTCCCGCCCG GGGACGAGCT CACCACCCGG CTCCAGCCTT TCCACAGCAC CGAGCTGGAG 
GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAGGGGGTG 
TCGGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCACTGC TGTCGTGCTC CCAGGAAGGA 
AGCCGCCACA CGAGCAAGCT CTCCCGCCAC GGCAGCGGCG CAGACAGTGA CTATGAGAAC 
ACACAAAGTG GGGACCCGCT GCTGGGCCTG GAAGGGAAGA GGTTTCTGGA GCTGGGCAAG 
GAAGAGGACT TCCACCCGGA GCTGGAAAGC CTGGATGGCG ACCTCGACCC TGGGCTTCCC 
AGCACGGAGG ATGTCATCCT GAAGACGGAG CAGGTCACCA AGAACATTCA GGAGTTACTG 
CGGGCGGCCC AGGAATTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCACTTG 
GCTGTGACTG AGATGGCCTC TCTCTTCCCG AAGAGGCCCG CCCTGGAGCC TGTGCGAAGC 
TCGCTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GCGAGTGCCG GAAGACGGTG 
CCCCCAGAGC CTGGCGCTCC CGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC 
GCCTACGACA TCGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAGAAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836459.1
CDS	165..2450
Translation	MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLGSPTDNLELSTRGQSDLDDQHDYDSVASDEDTDQEPLRSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALAASEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQIRQPPGPMPTPPLPSERAEHAPVGPGGSTHRRDRQAFSMYEPGSALKPFGVPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_024980691.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980691.1

ORF Insert Sequence:

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCCGGC 
TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT 
CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG 
CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CCGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG 
CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC 
GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTAT CCCTGGGCGC CCAGGCCAAC 
TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG 
CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC 
CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA ACGGCTAGTC 
GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT 
CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GCCTGGACCT GTCCGAGTTG 
GCCAAAGCTG CCAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTAGCC 
ATGGACGTGT ATGACGAGGT GGATCGAAGG GAAAATGACG CTGTGTGGCT GGCGACCCAG 
AACCACAGCA CCCTGGTGAC CGAGCGCAGC GCCGTGCCCT TCCTGCCAGT GAACCCCGAG 
TACTCAGCCA CACGGAATCA GGGGCGACAG AAGCTGGCCC GCTTTAACGC CCGGGAGTTT 
GCCACCTTGA TCATCGACAT CCTCAGCGAG GCCAAGAGGA GACAGCAGGG CAAGAGCCTG 
GGCAGCCCCA CAGACAACCT CGAGCTGTCC ACGCGGGGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTGGC CTCGGACGAG GACACAGACC AGGAGCCCCT GCGCAGTGCT 
GGTGCCACGC GGAACAACAG AGCTCGGAGC ATGGACTCCT CAGACCTGTC AGACGGGGCT 
GTGACGCTGC AAGAGTACCT GGAGCTGAAA AAGGCCCTGG CTGCTTCCGA GGCCAAGGTG 
CAGCAGCTCA TGAAGGTCAA TAGCAGCCTG AGTGATGAGC TCCGGAGGCT GCAGAGGGAG 
ATCCACAAGC TGCAGGCCGA GAACCTGCAG ATCCGGCAGC CACCAGGGCC GATGCCCACA 
CCCCCCCTCC CCAGCGAGCG AGCTGAGCAC GCACCCGTGG GGCCTGGCGG GAGCACCCAC 
CGCAGGGACC GCCAGGCCTT TTCCATGTAT GAACCAGGCT CTGCCCTGAA GCCCTTTGGG 
GTCCCGCCCG GGGACGAGCT CACCACCCGG CTCCAGCCTT TCCACAGCAC CGAGCTGGAG 
GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAGGGGGTG 
TCGGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCACTGC TGTCGTGCTC CCAGGAAGGA 
AGCCGCCACA CGAGCAAGCT CTCCCGCCAC GGCAGCGGCG CAGACAGTGA CTATGAGAAC 
ACACAAAGTG GGGACCCGCT GCTGGGCCTG GAAGGGAAGA GGTTTCTGGA GCTGGGCAAG 
GAAGAGGACT TCCACCCGGA GCTGGAAAGC CTGGATGGCG ACCTCGACCC TGGGCTTCCC 
AGCACGGAGG ATGTCATCCT GAAGACGGAG CAGGTCACCA AGAACATTCA GGAGTTACTG 
CGGGCGGCCC AGGAATTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCACTTG 
GCTGTGACTG AGATGGCCTC TCTCTTCCCG AAGAGGCCCG CCCTGGAGCC TGTGCGAAGC 
TCGCTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GCGAGTGCCG GAAGACGGTG 
CCCCCAGAGC CTGGCGCTCC CGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC 
GCCTACGACA TCGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAGAAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_024980693.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836461.1 Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226718
Clone ID Related Accession (Same CDS sequence)	XM_024980693.1
Accession Version	XM_024980693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCCGGC 
TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT 
CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG 
CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CCGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG 
CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC 
GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTAT CCCTGGGCGC CCAGGCCAAC 
TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG 
CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC 
CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA ACGGCTAGTC 
GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT 
CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG 
TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC 
AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA 
AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC 
GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG 
CTGGCCCGCT TTAACGCCCG GGAGTTTGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC 
AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCACG 
CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC 
ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACAGAGC TCGGAGCATG 
GACTCCTCAG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG 
GCCCTGGCTG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGT 
GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC 
CGGCAGCCAC CAGGGCCGAT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA 
CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA 
CCAGGCTCTG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC 
CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT 
GGCCTTTACC GGATCCGGAA GGGGGTGTCG GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC 
CCACTGCTGT CGTGCTCCCA GGAAGGAAGC CGCCACACGA GCAAGCTCTC CCGCCACGGC 
AGCGGCGCAG ACAGTGACTA TGAGAACACA CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA 
GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA GAGGACTTCC ACCCGGAGCT GGAAAGCCTG 
GATGGCGACC TCGACCCTGG GCTTCCCAGC ACGGAGGATG TCATCCTGAA GACGGAGCAG 
GTCACCAAGA ACATTCAGGA GTTACTGCGG GCGGCCCAGG AATTCAAGCA TGACAGCTTC 
GTGCCCTGCT CAGAGAAGAT CCACTTGGCT GTGACTGAGA TGGCCTCTCT CTTCCCGAAG 
AGGCCCGCCC TGGAGCCTGT GCGAAGCTCG CTGCGGCTGC TCAACGCCAG CGCCTACCGG 
CTGCAGAGCG AGTGCCGGAA GACGGTGCCC CCAGAGCCTG GCGCTCCCGT GGACTTCCAG 
CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TACGACATCG CCAAGGCCGC CAAGCAGCTG 
GTCACCATCA CCACCCGCGA GAAGAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836461.1
CDS	166..2478
Translation	MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLGSPTDNLELSTRGQSDLDDQHDYDSVASDEDTDQEPLRSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALAASEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQIRQPPGPMPTPPLPSERAEHAPVGPGGSTHRRDRQAFSMYEPGSALKPFGVPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_024980693.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980693.1

ORF Insert Sequence:

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCCGGC 
TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT 
CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG 
CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CCGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG 
CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC 
GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTAT CCCTGGGCGC CCAGGCCAAC 
TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG 
CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC 
CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA ACGGCTAGTC 
GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT 
CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG 
TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC 
AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA 
AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC 
GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG 
CTGGCCCGCT TTAACGCCCG GGAGTTTGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC 
AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCACG 
CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC 
ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACAGAGC TCGGAGCATG 
GACTCCTCAG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG 
GCCCTGGCTG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGT 
GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC 
CGGCAGCCAC CAGGGCCGAT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA 
CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA 
CCAGGCTCTG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC 
CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT 
GGCCTTTACC GGATCCGGAA GGGGGTGTCG GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC 
CCACTGCTGT CGTGCTCCCA GGAAGGAAGC CGCCACACGA GCAAGCTCTC CCGCCACGGC 
AGCGGCGCAG ACAGTGACTA TGAGAACACA CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA 
GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA GAGGACTTCC ACCCGGAGCT GGAAAGCCTG 
GATGGCGACC TCGACCCTGG GCTTCCCAGC ACGGAGGATG TCATCCTGAA GACGGAGCAG 
GTCACCAAGA ACATTCAGGA GTTACTGCGG GCGGCCCAGG AATTCAAGCA TGACAGCTTC 
GTGCCCTGCT CAGAGAAGAT CCACTTGGCT GTGACTGAGA TGGCCTCTCT CTTCCCGAAG 
AGGCCCGCCC TGGAGCCTGT GCGAAGCTCG CTGCGGCTGC TCAACGCCAG CGCCTACCGG 
CTGCAGAGCG AGTGCCGGAA GACGGTGCCC CCAGAGCCTG GCGCTCCCGT GGACTTCCAG 
CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TACGACATCG CCAAGGCCGC CAAGCAGCTG 
GTCACCATCA CCACCCGCGA GAAGAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015467970.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323456.1 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa95029
Clone ID Related Accession (Same CDS sequence)	XM_015467970.1
Accession Version	XM_015467970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 30% of CDS bases ##RefSeq-Attributes-END##

ATGGGTCCAC CAACCCCACA GACTGACTCC TCGGTGAGGG TGCACCACGG GGCGGCAGGT 
GGTGCCACCC ACCCCCCCGA CCCTACCCCA CCCCGCGAGG TATCTTTGCT CGCAGAAACC 
CTGCCAGATG AGCGGCTGGC TATGGGGGGA GGGGATGCCT GCAAGAAGAA TTTTTTTCTA 
AGGGGTCACT GTCCCTGGGC CCGAGCTCCT CAGGCCACCC TCAGCCTCGA ATATGAAGGG 
GACGAGGCTG TGAGGCGAAC TCGCAGCGTC TCGGCGGCAG GGGCGCACCG AGGCCCGGGC 
CGCAGGCGGC GCTGCGCGCT CGGGCCTGGC GGCGGCGGCG GCCCCTTTAA GCGCCCCCGC 
CCCCTCCCGC CCGCGCTCCC GGCTTCCTTT CTCCGGGAGG CGCGGCGGGC TGGGCCCGGC 
GGCGGGGCGG GTGTGCGGGG CCGCCCCTCC CGGGGCCGGG CCTGCGCCGC GTTCCTGCAG 
GAGCGGCCCG GAGCCGCGCA GGGGCGGAGA GAGCGAGCGG CGGTGGGGCG GCGCGGGCGG 
CGCGGGGCGG AGGGCGCCGA GGCGAGCGGC GGGCGGGCAC GGCGGGTTCG GGCCAGCCCG 
GGGGCGGACG GTTGGCCGGG CGCGCGGAGC CTAGCCGGGC CTTGCCGGAG CCGGGCCGGG 
CAGCAGGGGC CGCCGCCGCC CGGGCCCGCC GCCCGCGCCC CCCACGCTGG CCCAGAGGGC 
TGCGGCCGCG TCGCCGCTGA GGATGTCCGG AAGGGCCGCG AGCGAGGTGT GTGCGGACTG 
CAGGCGCCCC GGGTAAGCGG GCCGGGCTGG GGCGGGACCG TGCACCGAGG CGCCGCCGGC 
GGGCGCCGGG CCTGGCGGGC GCGCCGTGCG GGGCCGGAGG GCGGGGCGGG CTGGGGCTGC 
GGCCTCCGGC CTCGGGTCTC GCCCCCCCCG GCCGCTGCTG CGGAGCCGCG TCCTTGGAGG 
GAGGGCCGCT CGGGACGCGG TTTGCTCGCG TCCGTGACCA GCGAGGAAGA CATCCCCGGC 
GCCGCCTCCC GCCCCTCAGC TGGCGCCCCC CACCCTCCCC CGCTCCCCCC GCCTTCCATC 
TCTGGATCCA AACGGAAGCG CCAGCCTAGG GTGGCCAGGA GTACTGGCGG AGGCCGGCCG 
GGCTTCGGAG ACCCCGGCTG GGCCTCCATC AGCAGGGGTG TGCTGGTGTG TGACGAGTGC 
TGCAGCGTGC ACCGGAGTCT GGGCCGCCAC ATCTCCATTG TCAAGCACCT CCGCCACAGC 
GCCTGGCCCC CCACCCTGCT GCAGATGGTG CACACGCTTG CCAGCAACGG GGCCAACTCC 
ATCTGGGAGC ACTCCCTGCT GGACCCCGCG CAGGTGCAGA GCGGCCGGCG TAAAGCCAAC 
CCCCAAGACA AAGTCCACCC CATCAAGTCC GAGTTCATCA GGGCCAAGTA CCAGATGCTG 
GCTTTTGTGC ACAAGCTGCC CTGCCGGGAC GATGACGGAG TCACCGCCAA AGACCTCAGC 
AAGCAACTGC ACTCAAGCGT GCGAACGGGC AACCTGGAGA CATGTCTGCG CCTGCTATCC 
CTGGGCGCCC AGGCCAACTT CTTCCACCCC GAAAAGGGCA CCACACCTCT GCATGTGGCT 
GCCAAGGCAG GTCAGACGCT GCAGGCCGAG CTGCTTGTGG TGTATGGGGC TGACCCTGGC 
TCCCCAGATG TTAACGGCCG TACACCCATT GATTATGCCA GGCAGGCGGG GCACCATGAG 
CTGGCGGAAC GGCTAGTCGA GTGCCAGTAC GAGCTCACTG ACCGGTTGGC CTTCTATCTC 
TGTGGGCGCA AGCCGGATCA CAAGAATGGG CATTACATCA TCCCACAGAT GGCAGACAGA 
TCTCGGCAAA AGTGCATGTC TCAGAGCCTG GACCTGTCCG AGTTGGCCAA AGCTGCCAAG 
AAGAAGCTGC AGGCGCTCAG CAACCGGCTT TTTGAGGAAC TAGCCATGGA CGTGTATGAC 
GAGGTGGATC GAAGGGAAAA TGACGCTGTG TGGCTGGCGA CCCAGAACCA CAGCACCCTG 
GTGACCGAGC GCAGCGCCGT GCCCTTCCTG CCAGTGAACC CCGAGTACTC AGCCACACGG 
AATCAGGGGC GACAGAAGCT GGCCCGCTTT AACGCCCGGG AGTTTGCCAC CTTGATCATC 
GACATCCTCA GCGAGGCCAA GAGGAGACAG CAGGGCAAGA GCCTGGGCAG CCCCACAGAC 
AACCTCGAGC TGTCCACGCG GGGCCAGAGT GACCTGGACG ACCAGCACGA CTACGACAGC 
GTGGCCTCGG ACGAGGACAC AGACCAGGAG CCCCTGCGCA GTGCTGGTGC CACGCGGAAC 
AACAGAGCTC GGAGCATGGA CTCCTCAGAC CTGTCAGACG GGGCTGTGAC GCTGCAAGAG 
TACCTGGAGC TGAAAAAGGC CCTGGCTGCT TCCGAGGCCA AGGTGCAGCA GCTCATGAAG 
GTCAATAGCA GCCTGAGTGA TGAGCTCCGG AGGCTGCAGA GGGAGATCCA CAAGCTGCAG 
GCCGAGAACC TGCAGATCCG GCAGCCACCA GGGCCGATGC CCACACCCCC CCTCCCCAGC 
GAGCGAGCTG AGCACGCACC CGTGGGGCCT GGCGGGAGCA CCCACCGCAG GGACCGCCAG 
GCCTTTTCCA TGTATGAACC AGGCTCTGCC CTGAAGCCCT TTGGGGTCCC GCCCGGGGAC 
GAGCTCACCA CCCGGCTCCA GCCTTTCCAC AGCACCGAGC TGGAGGACGA CGCCATCTAT 
TCAGTGCACG TCCCTGCTGG CCTTTACCGG ATCCGGAAGG GGGTGTCGGC CTCAGCTGTG 
CCCTTCACTC CCTCCTCCCC ACTGCTGTCG TGCTCCCAGG AAGGAAGCCG CCACACGAGC 
AAGCTCTCCC GCCACGGCAG CGGCGCAGAC AGTGACTATG AGAACACACA AAGTGGGGAC 
CCGCTGCTGG GCCTGGAAGG GAAGAGGTTT CTGGAGCTGG GCAAGGAAGA GGACTTCCAC 
CCGGAGCTGG AAAGCCTGGA TGGCGACCTC GACCCTGGGC TTCCCAGCAC GGAGGATGTC 
ATCCTGAAGA CGGAGCAGGT CACCAAGAAC ATTCAGGAGT TACTGCGGGC GGCCCAGGAA 
TTCAAGCATG ACAGCTTCGT GCCCTGCTCA GAGAAGATCC ACTTGGCTGT GACTGAGATG 
GCCTCTCTCT TCCCGAAGAG GCCCGCCCTG GAGCCTGTGC GAAGCTCGCT GCGGCTGCTC 
AACGCCAGCG CCTACCGGCT GCAGAGCGAG TGCCGGAAGA CGGTGCCCCC AGAGCCTGGC 
GCTCCCGTGG ACTTCCAGCT GCTGACTCAG CAGGTGATCC AGTGCGCCTA CGACATCGCC 
AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323456.1
CDS	1..3411
Translation	MGPPTPQTDSSVRVHHGAAGGATHPPDPTPPREVSLLAETLPDERLAMGGGDACKKNFFLRGHCPWARAPQATLSLEYEGDEAVRRTRSVSAAGAHRGPGRRRRCALGPGGGGGPFKRPRPLPPALPASFLREARRAGPGGGAGVRGRPSRGRACAAFLQERPGAAQGRRERAAVGRRGRRGAEGAEASGGRARRVRASPGADGWPGARSLAGPCRSRAGQQGPPPPGPAARAPHAGPEGCGRVAAEDVRKGRERGVCGLQAPRVSGPGWGGTVHRGAAGGRRAWRARRAGPEGGAGWGCGLRPRVSPPPAAAAEPRPWREGRSGRGLLASVTSEEDIPGAASRPSAGAPHPPPLPPPSISGSKRKRQPRVARSTGGGRPGFGDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLGSPTDNLELSTRGQSDLDDQHDYDSVASDEDTDQEPLRSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALAASEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQIRQPPGPMPTPPLPSERAEHAPVGPGGSTHRRDRQAFSMYEPGSALKPFGVPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015467970.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467970.1

ORF Insert Sequence:

ATGGGTCCAC CAACCCCACA GACTGACTCC TCGGTGAGGG TGCACCACGG GGCGGCAGGT 
GGTGCCACCC ACCCCCCCGA CCCTACCCCA CCCCGCGAGG TATCTTTGCT CGCAGAAACC 
CTGCCAGATG AGCGGCTGGC TATGGGGGGA GGGGATGCCT GCAAGAAGAA TTTTTTTCTA 
AGGGGTCACT GTCCCTGGGC CCGAGCTCCT CAGGCCACCC TCAGCCTCGA ATATGAAGGG 
GACGAGGCTG TGAGGCGAAC TCGCAGCGTC TCGGCGGCAG GGGCGCACCG AGGCCCGGGC 
CGCAGGCGGC GCTGCGCGCT CGGGCCTGGC GGCGGCGGCG GCCCCTTTAA GCGCCCCCGC 
CCCCTCCCGC CCGCGCTCCC GGCTTCCTTT CTCCGGGAGG CGCGGCGGGC TGGGCCCGGC 
GGCGGGGCGG GTGTGCGGGG CCGCCCCTCC CGGGGCCGGG CCTGCGCCGC GTTCCTGCAG 
GAGCGGCCCG GAGCCGCGCA GGGGCGGAGA GAGCGAGCGG CGGTGGGGCG GCGCGGGCGG 
CGCGGGGCGG AGGGCGCCGA GGCGAGCGGC GGGCGGGCAC GGCGGGTTCG GGCCAGCCCG 
GGGGCGGACG GTTGGCCGGG CGCGCGGAGC CTAGCCGGGC CTTGCCGGAG CCGGGCCGGG 
CAGCAGGGGC CGCCGCCGCC CGGGCCCGCC GCCCGCGCCC CCCACGCTGG CCCAGAGGGC 
TGCGGCCGCG TCGCCGCTGA GGATGTCCGG AAGGGCCGCG AGCGAGGTGT GTGCGGACTG 
CAGGCGCCCC GGGTAAGCGG GCCGGGCTGG GGCGGGACCG TGCACCGAGG CGCCGCCGGC 
GGGCGCCGGG CCTGGCGGGC GCGCCGTGCG GGGCCGGAGG GCGGGGCGGG CTGGGGCTGC 
GGCCTCCGGC CTCGGGTCTC GCCCCCCCCG GCCGCTGCTG CGGAGCCGCG TCCTTGGAGG 
GAGGGCCGCT CGGGACGCGG TTTGCTCGCG TCCGTGACCA GCGAGGAAGA CATCCCCGGC 
GCCGCCTCCC GCCCCTCAGC TGGCGCCCCC CACCCTCCCC CGCTCCCCCC GCCTTCCATC 
TCTGGATCCA AACGGAAGCG CCAGCCTAGG GTGGCCAGGA GTACTGGCGG AGGCCGGCCG 
GGCTTCGGAG ACCCCGGCTG GGCCTCCATC AGCAGGGGTG TGCTGGTGTG TGACGAGTGC 
TGCAGCGTGC ACCGGAGTCT GGGCCGCCAC ATCTCCATTG TCAAGCACCT CCGCCACAGC 
GCCTGGCCCC CCACCCTGCT GCAGATGGTG CACACGCTTG CCAGCAACGG GGCCAACTCC 
ATCTGGGAGC ACTCCCTGCT GGACCCCGCG CAGGTGCAGA GCGGCCGGCG TAAAGCCAAC 
CCCCAAGACA AAGTCCACCC CATCAAGTCC GAGTTCATCA GGGCCAAGTA CCAGATGCTG 
GCTTTTGTGC ACAAGCTGCC CTGCCGGGAC GATGACGGAG TCACCGCCAA AGACCTCAGC 
AAGCAACTGC ACTCAAGCGT GCGAACGGGC AACCTGGAGA CATGTCTGCG CCTGCTATCC 
CTGGGCGCCC AGGCCAACTT CTTCCACCCC GAAAAGGGCA CCACACCTCT GCATGTGGCT 
GCCAAGGCAG GTCAGACGCT GCAGGCCGAG CTGCTTGTGG TGTATGGGGC TGACCCTGGC 
TCCCCAGATG TTAACGGCCG TACACCCATT GATTATGCCA GGCAGGCGGG GCACCATGAG 
CTGGCGGAAC GGCTAGTCGA GTGCCAGTAC GAGCTCACTG ACCGGTTGGC CTTCTATCTC 
TGTGGGCGCA AGCCGGATCA CAAGAATGGG CATTACATCA TCCCACAGAT GGCAGACAGA 
TCTCGGCAAA AGTGCATGTC TCAGAGCCTG GACCTGTCCG AGTTGGCCAA AGCTGCCAAG 
AAGAAGCTGC AGGCGCTCAG CAACCGGCTT TTTGAGGAAC TAGCCATGGA CGTGTATGAC 
GAGGTGGATC GAAGGGAAAA TGACGCTGTG TGGCTGGCGA CCCAGAACCA CAGCACCCTG 
GTGACCGAGC GCAGCGCCGT GCCCTTCCTG CCAGTGAACC CCGAGTACTC AGCCACACGG 
AATCAGGGGC GACAGAAGCT GGCCCGCTTT AACGCCCGGG AGTTTGCCAC CTTGATCATC 
GACATCCTCA GCGAGGCCAA GAGGAGACAG CAGGGCAAGA GCCTGGGCAG CCCCACAGAC 
AACCTCGAGC TGTCCACGCG GGGCCAGAGT GACCTGGACG ACCAGCACGA CTACGACAGC 
GTGGCCTCGG ACGAGGACAC AGACCAGGAG CCCCTGCGCA GTGCTGGTGC CACGCGGAAC 
AACAGAGCTC GGAGCATGGA CTCCTCAGAC CTGTCAGACG GGGCTGTGAC GCTGCAAGAG 
TACCTGGAGC TGAAAAAGGC CCTGGCTGCT TCCGAGGCCA AGGTGCAGCA GCTCATGAAG 
GTCAATAGCA GCCTGAGTGA TGAGCTCCGG AGGCTGCAGA GGGAGATCCA CAAGCTGCAG 
GCCGAGAACC TGCAGATCCG GCAGCCACCA GGGCCGATGC CCACACCCCC CCTCCCCAGC 
GAGCGAGCTG AGCACGCACC CGTGGGGCCT GGCGGGAGCA CCCACCGCAG GGACCGCCAG 
GCCTTTTCCA TGTATGAACC AGGCTCTGCC CTGAAGCCCT TTGGGGTCCC GCCCGGGGAC 
GAGCTCACCA CCCGGCTCCA GCCTTTCCAC AGCACCGAGC TGGAGGACGA CGCCATCTAT 
TCAGTGCACG TCCCTGCTGG CCTTTACCGG ATCCGGAAGG GGGTGTCGGC CTCAGCTGTG 
CCCTTCACTC CCTCCTCCCC ACTGCTGTCG TGCTCCCAGG AAGGAAGCCG CCACACGAGC 
AAGCTCTCCC GCCACGGCAG CGGCGCAGAC AGTGACTATG AGAACACACA AAGTGGGGAC 
CCGCTGCTGG GCCTGGAAGG GAAGAGGTTT CTGGAGCTGG GCAAGGAAGA GGACTTCCAC 
CCGGAGCTGG AAAGCCTGGA TGGCGACCTC GACCCTGGGC TTCCCAGCAC GGAGGATGTC 
ATCCTGAAGA CGGAGCAGGT CACCAAGAAC ATTCAGGAGT TACTGCGGGC GGCCCAGGAA 
TTCAAGCATG ACAGCTTCGT GCCCTGCTCA GAGAAGATCC ACTTGGCTGT GACTGAGATG 
GCCTCTCTCT TCCCGAAGAG GCCCGCCCTG GAGCCTGTGC GAAGCTCGCT GCGGCTGCTC 
AACGCCAGCG CCTACCGGCT GCAGAGCGAG TGCCGGAAGA CGGTGCCCCC AGAGCCTGGC 
GCTCCCGTGG ACTTCCAGCT GCTGACTCAG CAGGTGATCC AGTGCGCCTA CGACATCGCC 
AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GIT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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