DENND4A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005211325.3	XP_005211382.1	C-myc promoter-binding protein isoform X4
XM_005211320.3	XP_005211377.1	C-myc promoter-binding protein isoform X1
XM_005211320.4	XP_005211377.1	C-myc promoter-binding protein isoform X1
XM_005211323.4	XP_005211380.1	C-myc promoter-binding protein isoform X2
NM_001205525.3	NP_001192454.2	C-myc promoter-binding protein
XM_005211319.3	XP_005211376.1	C-myc promoter-binding protein isoform X1
XM_015473032.1	XP_015328518.1	C-myc promoter-binding protein isoform X1
XM_005211323.3	XP_005211380.1	C-myc promoter-binding protein isoform X2
XM_005211324.4	XP_005211381.1	C-myc promoter-binding protein isoform X3
XM_005211325.4	XP_005211382.1	C-myc promoter-binding protein isoform X4
XM_024997490.1	XP_024853258.1	C-myc promoter-binding protein isoform X6
NM_001205525.1	NP_001192454.1	C-myc promoter-binding protein
NM_001205525.2	NP_001192454.2	C-myc promoter-binding protein
XM_005211324.3	XP_005211381.1	C-myc promoter-binding protein isoform X3
XM_005211319.4	XP_005211376.1	C-myc promoter-binding protein isoform X1
XM_024997489.1	XP_024853257.1	C-myc promoter-binding protein isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219529	XM_024997489.1 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219530	XM_024997490.1 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa22956	NM_001205525.3 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa39499	NM_001205525.1 Latest version!	Bos taurus DENN/MADD domain containing 4A (DENND4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22953	XM_005211319.4 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22953	XM_015473032.1 Latest version!	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22953	XM_005211320.4 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22954	XM_005211323.4 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22955	XM_005211324.4 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22956	XM_005211325.4 Latest version!	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa22953	XM_005211320.3	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22954	XM_005211323.3	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22956	XM_005211325.3	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22956	NM_001205525.2	Bos taurus DENN domain containing 4A (DENND4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22955	XM_005211324.3	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa22953	XM_005211319.3	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

DENND4A ( XM_024997489.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853257.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa219529
Clone ID Related Accession (Same CDS sequence)	XM_024997489.1
Accession Version	XM_024997489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACTG CAAAGACCAA CGCAATAAGA ACAGATGACT ATTTACTAAA CATCAACAAG 
TATTTCTCAA ACTCTGAGGC TTGCAGAATT CACAAATTGA GATCTGGCGT CATTTTCAGA 
GAGACTTTCA GTAATGTGGT TTTATATGAC TGGAAAGAAA GATTGAAACA AGGGTGTGAA 
ATTATCCAGA GTACTCCCTA TGGACGCCCT GCAAATATTA GCGGCAGTAC CTCATCACAA 
AGAATTTATA TCACTTACCG AAGAGCCTCT GAAAACATGA CTCAGAATAC GTTGGCTGTT 
ACAGACATAT GTATTATTAT ACCCAGTAAA GGAGAAAGTC CACCACACAC ATTCTGCAAA 
GTTGACAAGA ATCTCAATAA CAGTATGTGG GGATCAGCAG TATACTTATG TTATAAAAAA 
TCAGTGGCAA AGACTAACAC TATATCTTAC AAAGCTGGTC TAATTTGTAG ATACCCTCAA 
GAAGATTACG AGTCATTCTC ACTCCCAGAA TCTGTTCCCC TGTTTTGTTT ACCAATGGGC 
GCAACTATTG AATGTTGGCC ATCAAATAGC AAATACCCCC TCCCTGTATT TTCTACATTC 
GTGTTAACTG GAGCCTCAGC TGAAAAGGTC TATGGTGCTG CTATTCAGTT TTATGAACCA 
TACTCCGAGG AGAATCTCAC AGAAAAACAG AGATTTCTTT TGGGTTTAGC ATCATCAGCA 
AATGAGAAGT CTGATAGTTC CAAGACAATT CACACTAATA AATGCATCTG TCTTCTCTCT 
CACTGGCCTT TTTTTGACGC ATTCAGGAAG TTTTTGACTT TTCTATATCG TTATTCCATC 
TCTGGACCTC ACGTCCTTCC AATTGAGAAG CATATTTCTC ATTTTATGCA TAAAGTTCCT 
TTTCCATCTC CTCAGAGACC ACGGATTCTA GTTCAGTTAT CACCACATGA TAATCTGATC 
CTCAGTCAGC CTGTTTCTTC ACCTCTTCCA CTAAGTGGTG GCAAGTTTTC AACACTGCTC 
CAGAATTTAG GCCCTGAAAA TGCTGTGACA CTCCTGGTGT TTGCAGTAAC AGAACATAAA 
ATTCTCATTC ATTCCTTACG GCCTTCCGTG CTTACCAGTG TGACAGAAGC ATTAGTGTCT 
ATGATTTTCC CTTTCCACTG GCCATGCCCC TATGTTCCTC TCTGCCCACT GGCTTTAGCA 
GATGTCTTGA GTGCACCATG TCCATTCATA GTAGGAATTG ATTCAAGATA CTTTGATCTC 
TATGACCCTC CACCAGATGT CAGTTGTGTT GACCTAGATA CCAACACCAT TTCTCAAACT 
GGTGACAAGA AAAATGTAGC CTGGAAGATA CTTCCAAAGA AACCATGTAA AAATCTGATG 
AACACACTGA ATAATTTGCA TCAACAACTG GCAAAATTAC AGCAGAGACC AAGAGATGAT 
GGACTTATGG ACTTAGCAAT GAATGATTAT GACTTTAATT CTGGAAAGAG GTTGCACATG 
ATAGACTTGG AAATCCAAGA GGCATTTTTG TGTTTCATGG CCTCTATTTT AAAAGGTTAT 
AGATCATACT TAAGACCAAT AACTCAAGCC CCATCTGAGA CAGCCACAGA TGCAACTTCT 
CTTTTTGCTC TACAAGCTTT CTTAAGAAGT CGGGACCGGT CACATCAGAA ATTCTATAAC 
ATGATGACCA AAACACAAAT GTTTATTCGC TTCATTGAGG AATGTTCTTT CGTCAGTGAT 
AAAGATGCAA GTCTGGCGTT TTTTGATGAT TGTGTAGATA AAGTGGATAT GGACAAGAGT 
GGTGAAGTGC GACTTATAGA ATTGGATGAA TCTTTCAAAA GTGAACATAC TGTTTTTGTA 
ACACCTCCTG AGATCCCCCA TCTACCCAAT GGTGAAGAAC CCCCTTTACA GTACAGCTAT 
AATGGATTTC CAGTTCTTAG GAACAATTTA TTTGAGAGAC CTGAAGGATT TCTGCAAACA 
CGAAAGAGCA AATTGCCTTC AAAATCAAGT AGTCCTAGTA GCCCTTCTCC CATGTTCAGA 
AGAACGAAAC AGGAAATTAA ATCAGCCCAT AAAATTGCCA AGAGATATTC TTCCATTCCT 
CAGATGTGGT CTAGGTGTCT ACTGCGTCAC TGTTATGGAC TCTGGTTTAT TTGTCTCCCA 
GCTTATGTGA AAGTATGCCA TTCAAAAGTC AGGGCTCTGA AAACAGCATA TGATGTACTC 
AAAAAAATGC AGTCAAAGAA GATGGATCCA CCTGATGAGG TGTGCTACCG CATTCTCATG 
CAGCTCTGCG GACAGTATGA TCAGCCAGTG CTTGCAGTTC GAGTGCTTTT TGAAATGCAG 
AAAGCTGGTA TTGACCCAAA TGCTATTACT TATGGCTATT ATAATAAGGC TGTTTTGGAA 
AGTACTTGGC CTTCAAGGAG TCGTAGTGGA TATTTTCTTT GGACAAAAGT GAGAAATGTT 
GTTTTAGGAG TAGCACAGTT CAAAAGAGCT TTAAAGAAGC ATGCGAATTT ATCTCAAACA 
GCTCTCTCAG CAGATGGCAG TGACCTGGAC GCTGTTAGCC ATGGCAGCAT GGATAGTGGC 
CATGGGACAC ACGCTGTGGA GCAGGCGCCG TGTAGTACAG GTTCAATCAA AGCGTATGCT 
TCCGATGATA GATCTAGTAC AGGTGGCCAG TCTGACCTTG GATATAATTC GTTATCTAAG 
GATGAAGTTA GAAGAGGGGA TACATCCACT GAGGACATTC AAGAAGAAAA AGATAAGAAA 
GGGAGTGATT CTAGTTCCTT GTCAGAGTGC GAGAGTGCAA AGGGAAGTGC CGACTGTCTT 
CCTAAGCTCG GTTATCAAAG TTCTTCCAGT ATAGTTCGCC TCAATGGTAC AAGTAACAGT 
GCTGATAAAA TATCAGGAGA AAGCACAGAA AGCACATCTG AGCCGCTCTT AATATCGTCT 
TTTGAGGATG CAAATGAAAC AGGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AGCCTGATTT GCAGCAGCAA ATGTCCTGGG TAAATCGAAG CCGCAACCTT 
AGTGGAGGAG TGCTGATGGG ACTTATGCTC AGTAGAATTA ACCAGGAAGC AAACCCTGGA 
AACATGGTTG AGAAATTAGG AGCTGATGCA AAAATTCTTT CAAATGTAAT CTCAAAAAGC 
ACAAGACCAA ATAGTCTTAA TATTGGAAAA CCGTCTTTAA GATCAAAGAG AGACAGTGTA 
GAAAAGGAAT CGAGTGATGA TGATACACCT TTTGATGGTT CTAATTGTCT GACAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA ATGAAACAGA TATTTCAAAA 
GCGAGGTACA TTGTTCCACA AAATCCCAAG AGGAGTCAAC GCTTGAACAG CAGCTTTTCA 
ATAAAACCTT TTGAAAAAAC TGATGTTGCC ACAGGATTTG ATCCCCTCTC ACTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAGGAG GAGGAAGAGG AGGAGGATGA CAGCAAAAGT 
GTTTCAACAC CATCTGCTAG GCGGGATTTA GCTGAAGAAA TTGAGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGAACACCA AGCATTGATT TACAACGGGC CTGTGATGAT 
AAAGTCACCA CTAAGAAAAG TCCAACGTTG GTCAAGGCAT GCAGAAGATC TAGCCTGCCT 
CCTAATTCTC CCAGACCAGT GAGACTTACC AAATCCAAAA GTTACTCAAA AAATGAGGAC 
AGGCCAAGGG ATAGACTCTG GTCTTCTCCA GCCTTCTCAC CTACCTGTCC ATTTAGGGAA 
GAATCTCAAG ATGCATTAAC CCATTCATCA CCTTCATTTA ATTTAGACAC ACTACTAGTA 
CCTAAACTAG ATGTTCTGAG AAACAGTATG TTCACTGCTG GAAAAGGAGT TGCCGAGAAA 
GCAAGCAAAT GGTATTCAAG ATTCACCATG TACACCACAT CATCAAAGGA TCAAAGTTCT 
GACCGTACCA GTCTTTCTTC AGTGGGAGCC CAGGATTCTG AATCTACCTC TTTAACAGAT 
GAAGATGTCT GCCATGAGTT GGAAGGAGCC ACTTCTCAGG AGAGCAGCGC CACTTCAGGG 
ACTAAGGGAG TTGATTTCAG CAGAACAAGC CTGGAAGGCT CTGCCTCCTT AGAAGGATCC 
TTATCCAAGT TTGCCTTACC TGGGAAATCA GAGGTGGCAT CTTCCTGCAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTCCTTATCT CAAGCTGCTC TCGGTGTAAA 
ACCTGCGATT GTCTGGTCCA CGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CTACCTGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTCT GAATATAGAG 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTTTGAAGT CAAGCCCATC TACAGAAAAT 
ATACATTTTC CATCCTCCTT TTCAAGTCAG ACAAGGCAGT CTTGCATCTC AACATCAACC 
TCTGGTCTTG ACACATCTCT TCTCTCTGTT CACGGGAGTT TTGATCTGAA TAGCAAATCT 
AAGCCGCTGG AAAATACATG TGCCCGAAGC ATTCAGATCC CTGCTGATAG ATCAAAAGCA 
GCTATGTCAA AATGTCCGTT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCACCTTG 
GATCAGGAAG ACATTGGCGG GCAGAAGCTC GTTCCTACAG GCAGCCTGCC AACCACCTTA 
CAAGGCGCTG CGGATTCTTT AGGATTAGAA TGGCACCTCC CAAGTCCAGA TCCTATCACT 
GTTCCGTACC TTAGCCCACT AGTGGTGTGG AAGGAACTTG AAAGCTTACT GGAAAACGAA 
GGTGATCGTG CAATAACAGT GGCAGACTTC GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTTGACTTA CCCAGTAATT TACCTGGACT GATTCTTTCT 
TCTGAGCATT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCTGA AGACAGTAAA 
TATGTTTTAA TACAGATGTT GTGGGACAAT ATGAAATTAC ATCAGGATCC AGGACAGCCC 
TTGTACATCC TCTGGAATGC GCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAAGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAC 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTGG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAT 
AAGATGGCAT ATGATCGTCT CACACCCAGT CAAGTCAAGA GCACCCACAA CTGCGACAGG 
CCGCCAAGCA CAGGGGTGAT GGAATGTCGG AAGACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853257.1
CDS	201..5777
Translation	MRTAKTNAIRTDDYLLNINKYFSNSEACRIHKLRSGVIFRETFSNVVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_024997489.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997489.1

ORF Insert Sequence:

ATGAGAACTG CAAAGACCAA CGCAATAAGA ACAGATGACT ATTTACTAAA CATCAACAAG 
TATTTCTCAA ACTCTGAGGC TTGCAGAATT CACAAATTGA GATCTGGCGT CATTTTCAGA 
GAGACTTTCA GTAATGTGGT TTTATATGAC TGGAAAGAAA GATTGAAACA AGGGTGTGAA 
ATTATCCAGA GTACTCCCTA TGGACGCCCT GCAAATATTA GCGGCAGTAC CTCATCACAA 
AGAATTTATA TCACTTACCG AAGAGCCTCT GAAAACATGA CTCAGAATAC GTTGGCTGTT 
ACAGACATAT GTATTATTAT ACCCAGTAAA GGAGAAAGTC CACCACACAC ATTCTGCAAA 
GTTGACAAGA ATCTCAATAA CAGTATGTGG GGATCAGCAG TATACTTATG TTATAAAAAA 
TCAGTGGCAA AGACTAACAC TATATCTTAC AAAGCTGGTC TAATTTGTAG ATACCCTCAA 
GAAGATTACG AGTCATTCTC ACTCCCAGAA TCTGTTCCCC TGTTTTGTTT ACCAATGGGC 
GCAACTATTG AATGTTGGCC ATCAAATAGC AAATACCCCC TCCCTGTATT TTCTACATTC 
GTGTTAACTG GAGCCTCAGC TGAAAAGGTC TATGGTGCTG CTATTCAGTT TTATGAACCA 
TACTCCGAGG AGAATCTCAC AGAAAAACAG AGATTTCTTT TGGGTTTAGC ATCATCAGCA 
AATGAGAAGT CTGATAGTTC CAAGACAATT CACACTAATA AATGCATCTG TCTTCTCTCT 
CACTGGCCTT TTTTTGACGC ATTCAGGAAG TTTTTGACTT TTCTATATCG TTATTCCATC 
TCTGGACCTC ACGTCCTTCC AATTGAGAAG CATATTTCTC ATTTTATGCA TAAAGTTCCT 
TTTCCATCTC CTCAGAGACC ACGGATTCTA GTTCAGTTAT CACCACATGA TAATCTGATC 
CTCAGTCAGC CTGTTTCTTC ACCTCTTCCA CTAAGTGGTG GCAAGTTTTC AACACTGCTC 
CAGAATTTAG GCCCTGAAAA TGCTGTGACA CTCCTGGTGT TTGCAGTAAC AGAACATAAA 
ATTCTCATTC ATTCCTTACG GCCTTCCGTG CTTACCAGTG TGACAGAAGC ATTAGTGTCT 
ATGATTTTCC CTTTCCACTG GCCATGCCCC TATGTTCCTC TCTGCCCACT GGCTTTAGCA 
GATGTCTTGA GTGCACCATG TCCATTCATA GTAGGAATTG ATTCAAGATA CTTTGATCTC 
TATGACCCTC CACCAGATGT CAGTTGTGTT GACCTAGATA CCAACACCAT TTCTCAAACT 
GGTGACAAGA AAAATGTAGC CTGGAAGATA CTTCCAAAGA AACCATGTAA AAATCTGATG 
AACACACTGA ATAATTTGCA TCAACAACTG GCAAAATTAC AGCAGAGACC AAGAGATGAT 
GGACTTATGG ACTTAGCAAT GAATGATTAT GACTTTAATT CTGGAAAGAG GTTGCACATG 
ATAGACTTGG AAATCCAAGA GGCATTTTTG TGTTTCATGG CCTCTATTTT AAAAGGTTAT 
AGATCATACT TAAGACCAAT AACTCAAGCC CCATCTGAGA CAGCCACAGA TGCAACTTCT 
CTTTTTGCTC TACAAGCTTT CTTAAGAAGT CGGGACCGGT CACATCAGAA ATTCTATAAC 
ATGATGACCA AAACACAAAT GTTTATTCGC TTCATTGAGG AATGTTCTTT CGTCAGTGAT 
AAAGATGCAA GTCTGGCGTT TTTTGATGAT TGTGTAGATA AAGTGGATAT GGACAAGAGT 
GGTGAAGTGC GACTTATAGA ATTGGATGAA TCTTTCAAAA GTGAACATAC TGTTTTTGTA 
ACACCTCCTG AGATCCCCCA TCTACCCAAT GGTGAAGAAC CCCCTTTACA GTACAGCTAT 
AATGGATTTC CAGTTCTTAG GAACAATTTA TTTGAGAGAC CTGAAGGATT TCTGCAAACA 
CGAAAGAGCA AATTGCCTTC AAAATCAAGT AGTCCTAGTA GCCCTTCTCC CATGTTCAGA 
AGAACGAAAC AGGAAATTAA ATCAGCCCAT AAAATTGCCA AGAGATATTC TTCCATTCCT 
CAGATGTGGT CTAGGTGTCT ACTGCGTCAC TGTTATGGAC TCTGGTTTAT TTGTCTCCCA 
GCTTATGTGA AAGTATGCCA TTCAAAAGTC AGGGCTCTGA AAACAGCATA TGATGTACTC 
AAAAAAATGC AGTCAAAGAA GATGGATCCA CCTGATGAGG TGTGCTACCG CATTCTCATG 
CAGCTCTGCG GACAGTATGA TCAGCCAGTG CTTGCAGTTC GAGTGCTTTT TGAAATGCAG 
AAAGCTGGTA TTGACCCAAA TGCTATTACT TATGGCTATT ATAATAAGGC TGTTTTGGAA 
AGTACTTGGC CTTCAAGGAG TCGTAGTGGA TATTTTCTTT GGACAAAAGT GAGAAATGTT 
GTTTTAGGAG TAGCACAGTT CAAAAGAGCT TTAAAGAAGC ATGCGAATTT ATCTCAAACA 
GCTCTCTCAG CAGATGGCAG TGACCTGGAC GCTGTTAGCC ATGGCAGCAT GGATAGTGGC 
CATGGGACAC ACGCTGTGGA GCAGGCGCCG TGTAGTACAG GTTCAATCAA AGCGTATGCT 
TCCGATGATA GATCTAGTAC AGGTGGCCAG TCTGACCTTG GATATAATTC GTTATCTAAG 
GATGAAGTTA GAAGAGGGGA TACATCCACT GAGGACATTC AAGAAGAAAA AGATAAGAAA 
GGGAGTGATT CTAGTTCCTT GTCAGAGTGC GAGAGTGCAA AGGGAAGTGC CGACTGTCTT 
CCTAAGCTCG GTTATCAAAG TTCTTCCAGT ATAGTTCGCC TCAATGGTAC AAGTAACAGT 
GCTGATAAAA TATCAGGAGA AAGCACAGAA AGCACATCTG AGCCGCTCTT AATATCGTCT 
TTTGAGGATG CAAATGAAAC AGGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AGCCTGATTT GCAGCAGCAA ATGTCCTGGG TAAATCGAAG CCGCAACCTT 
AGTGGAGGAG TGCTGATGGG ACTTATGCTC AGTAGAATTA ACCAGGAAGC AAACCCTGGA 
AACATGGTTG AGAAATTAGG AGCTGATGCA AAAATTCTTT CAAATGTAAT CTCAAAAAGC 
ACAAGACCAA ATAGTCTTAA TATTGGAAAA CCGTCTTTAA GATCAAAGAG AGACAGTGTA 
GAAAAGGAAT CGAGTGATGA TGATACACCT TTTGATGGTT CTAATTGTCT GACAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA ATGAAACAGA TATTTCAAAA 
GCGAGGTACA TTGTTCCACA AAATCCCAAG AGGAGTCAAC GCTTGAACAG CAGCTTTTCA 
ATAAAACCTT TTGAAAAAAC TGATGTTGCC ACAGGATTTG ATCCCCTCTC ACTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAGGAG GAGGAAGAGG AGGAGGATGA CAGCAAAAGT 
GTTTCAACAC CATCTGCTAG GCGGGATTTA GCTGAAGAAA TTGAGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGAACACCA AGCATTGATT TACAACGGGC CTGTGATGAT 
AAAGTCACCA CTAAGAAAAG TCCAACGTTG GTCAAGGCAT GCAGAAGATC TAGCCTGCCT 
CCTAATTCTC CCAGACCAGT GAGACTTACC AAATCCAAAA GTTACTCAAA AAATGAGGAC 
AGGCCAAGGG ATAGACTCTG GTCTTCTCCA GCCTTCTCAC CTACCTGTCC ATTTAGGGAA 
GAATCTCAAG ATGCATTAAC CCATTCATCA CCTTCATTTA ATTTAGACAC ACTACTAGTA 
CCTAAACTAG ATGTTCTGAG AAACAGTATG TTCACTGCTG GAAAAGGAGT TGCCGAGAAA 
GCAAGCAAAT GGTATTCAAG ATTCACCATG TACACCACAT CATCAAAGGA TCAAAGTTCT 
GACCGTACCA GTCTTTCTTC AGTGGGAGCC CAGGATTCTG AATCTACCTC TTTAACAGAT 
GAAGATGTCT GCCATGAGTT GGAAGGAGCC ACTTCTCAGG AGAGCAGCGC CACTTCAGGG 
ACTAAGGGAG TTGATTTCAG CAGAACAAGC CTGGAAGGCT CTGCCTCCTT AGAAGGATCC 
TTATCCAAGT TTGCCTTACC TGGGAAATCA GAGGTGGCAT CTTCCTGCAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTCCTTATCT CAAGCTGCTC TCGGTGTAAA 
ACCTGCGATT GTCTGGTCCA CGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CTACCTGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTCT GAATATAGAG 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTTTGAAGT CAAGCCCATC TACAGAAAAT 
ATACATTTTC CATCCTCCTT TTCAAGTCAG ACAAGGCAGT CTTGCATCTC AACATCAACC 
TCTGGTCTTG ACACATCTCT TCTCTCTGTT CACGGGAGTT TTGATCTGAA TAGCAAATCT 
AAGCCGCTGG AAAATACATG TGCCCGAAGC ATTCAGATCC CTGCTGATAG ATCAAAAGCA 
GCTATGTCAA AATGTCCGTT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCACCTTG 
GATCAGGAAG ACATTGGCGG GCAGAAGCTC GTTCCTACAG GCAGCCTGCC AACCACCTTA 
CAAGGCGCTG CGGATTCTTT AGGATTAGAA TGGCACCTCC CAAGTCCAGA TCCTATCACT 
GTTCCGTACC TTAGCCCACT AGTGGTGTGG AAGGAACTTG AAAGCTTACT GGAAAACGAA 
GGTGATCGTG CAATAACAGT GGCAGACTTC GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTTGACTTA CCCAGTAATT TACCTGGACT GATTCTTTCT 
TCTGAGCATT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCTGA AGACAGTAAA 
TATGTTTTAA TACAGATGTT GTGGGACAAT ATGAAATTAC ATCAGGATCC AGGACAGCCC 
TTGTACATCC TCTGGAATGC GCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAAGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAC 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTGG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAT 
AAGATGGCAT ATGATCGTCT CACACCCAGT CAAGTCAAGA GCACCCACAA CTGCGACAGG 
CCGCCAAGCA CAGGGGTGAT GGAATGTCGG AAGACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_024997490.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853258.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

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CloneID	OBa219530
Clone ID Related Accession (Same CDS sequence)	XM_024997490.1
Accession Version	XM_024997490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC AGTATTTCTC AAACTCTGAG GCTTGCAGAA TTCACAAATT GAGATCTGGC 
GTCATTTTCA GAGAGACTTT CAGTAATGTG GTTTTATATG ACTGGAAAGA AAGATTGAAA 
CAAGGGTGTG AAATTATCCA GAGTACTCCC TATGGACGCC CTGCAAATAT TAGCGGCAGT 
ACCTCATCAC AAAGAATTTA TATCACTTAC CGAAGAGCCT CTGAAAACAT GACTCAGAAT 
ACGTTGGCTG TTACAGACAT ATGTATTATT ATACCCAGTA AAGGAGAAAG TCCACCACAC 
ACATTCTGCA AAGTTGACAA GAATCTCAAT AACAGTATGT GGGGATCAGC AGTATACTTA 
TGTTATAAAA AATCAGTGGC AAAGACTAAC ACTATATCTT ACAAAGCTGG TCTAATTTGT 
AGATACCCTC AAGAAGATTA CGAGTCATTC TCACTCCCAG AATCTGTTCC CCTGTTTTGT 
TTACCAATGG GCGCAACTAT TGAATGTTGG CCATCAAATA GCAAATACCC CCTCCCTGTA 
TTTTCTACAT TCGTGTTAAC TGGAGCCTCA GCTGAAAAGG TCTATGGTGC TGCTATTCAG 
TTTTATGAAC CATACTCCGA GGAGAATCTC ACAGAAAAAC AGAGATTTCT TTTGGGTTTA 
GCATCATCAG CAAATGAGAA GTCTGATAGT TCCAAGACAA TTCACACTAA TAAATGCATC 
TGTCTTCTCT CTCACTGGCC TTTTTTTGAC GCATTCAGGA AGTTTTTGAC TTTTCTATAT 
CGTTATTCCA TCTCTGGACC TCACGTCCTT CCAATTGAGA AGCATATTTC TCATTTTATG 
CATAAAGTTC CTTTTCCATC TCCTCAGAGA CCACGGATTC TAGTTCAGTT ATCACCACAT 
GATAATCTGA TCCTCAGTCA GCCTGTTTCT TCACCTCTTC CACTAAGTGG TGGCAAGTTT 
TCAACACTGC TCCAGAATTT AGGCCCTGAA AATGCTGTGA CACTCCTGGT GTTTGCAGTA 
ACAGAACATA AAATTCTCAT TCATTCCTTA CGGCCTTCCG TGCTTACCAG TGTGACAGAA 
GCATTAGTGT CTATGATTTT CCCTTTCCAC TGGCCATGCC CCTATGTTCC TCTCTGCCCA 
CTGGCTTTAG CAGATGTCTT GAGTGCACCA TGTCCATTCA TAGTAGGAAT TGATTCAAGA 
TACTTTGATC TCTATGACCC TCCACCAGAT GTCAGTTGTG TTGACCTAGA TACCAACACC 
ATTTCTCAAA CTGGTGACAA GAAAAATGTA GCCTGGAAGA TACTTCCAAA GAAACCATGT 
AAAAATCTGA TGAACACACT GAATAATTTG CATCAACAAC TGGCAAAATT ACAGCAGAGA 
CCAAGAGATG ATGGACTTAT GGACTTAGCA ATGAATGATT ATGACTTTAA TTCTGGAAAG 
AGGTTGCACA TGATAGACTT GGAAATCCAA GAGGCATTTT TGTGTTTCAT GGCCTCTATT 
TTAAAAGGTT ATAGATCATA CTTAAGACCA ATAACTCAAG CCCCATCTGA GACAGCCACA 
GATGCAACTT CTCTTTTTGC TCTACAAGCT TTCTTAAGAA GTCGGGACCG GTCACATCAG 
AAATTCTATA ACATGATGAC CAAAACACAA ATGTTTATTC GCTTCATTGA GGAATGTTCT 
TTCGTCAGTG ATAAAGATGC AAGTCTGGCG TTTTTTGATG ATTGTGTAGA TAAAGTGGAT 
ATGGACAAGA GTGGTGAAGT GCGACTTATA GAATTGGATG AATCTTTCAA AAGTGAACAT 
ACTGTTTTTG TAACACCTCC TGAGATCCCC CATCTACCCA ATGGTGAAGA ACCCCCTTTA 
CAGTACAGCT ATAATGGATT TCCAGTTCTT AGGAACAATT TATTTGAGAG ACCTGAAGGA 
TTTCTGCAAA CACGAAAGAG CAAATTGCCT TCAAAATCAA GTAGTCCTAG TAGCCCTTCT 
CCCATGTTCA GAAGAACGAA ACAGGAAATT AAATCAGCCC ATAAAATTGC CAAGAGATAT 
TCTTCCATTC CTCAGATGTG GTCTAGGTGT CTACTGCGTC ACTGTTATGG ACTCTGGTTT 
ATTTGTCTCC CAGCTTATGT GAAAGTATGC CATTCAAAAG TCAGGGCTCT GAAAACAGCA 
TATGATGTAC TCAAAAAAAT GCAGTCAAAG AAGATGGATC CACCTGATGA GGTGTGCTAC 
CGCATTCTCA TGCAGCTCTG CGGACAGTAT GATCAGCCAG TGCTTGCAGT TCGAGTGCTT 
TTTGAAATGC AGAAAGCTGG TATTGACCCA AATGCTATTA CTTATGGCTA TTATAATAAG 
GCTGTTTTGG AAAGTACTTG GCCTTCAAGG AGTCGTAGTG GATATTTTCT TTGGACAAAA 
GTGAGAAATG TTGTTTTAGG AGTAGCACAG TTCAAAAGAG CTTTAAAGAA GCATGCGAAT 
TTATCTCAAA CAGCTCTCTC AGCAGATGGC AGTGACCTGG ACGCTGTTAG CCATGGCAGC 
ATGGATAGTG GCCATGGGAC ACACGCTGTG GAGCAGGCGC CGTGTAGTAC AGGTTCAATC 
AAAGCGTATG CTTCCGATGA TAGATCTAGT ACAGGTGGCC AGTCTGACCT TGGATATAAT 
TCGTTATCTA AGGATGAAGT TAGAAGAGGG GATACATCCA CTGAGGACAT TCAAGAAGAA 
AAAGATAAGA AAGGGAGTGA TTCTAGTTCC TTGTCAGAGT GCGAGAGTGC AAAGGGAAGT 
GCCGACTGTC TTCCTAAGCT CGGTTATCAA AGTTCTTCCA GTATAGTTCG CCTCAATGGT 
ACAAGTAACA GTGCTGATAA AATATCAGGA GAAAGCACAG AAAGCACATC TGAGCCGCTC 
TTAATATCGT CTTTTGAGGA TGCAAATGAA ACAGGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAGCCTGAT TTGCAGCAGC AAATGTCCTG GGTAAATCGA 
AGCCGCAACC TTAGTGGAGG AGTGCTGATG GGACTTATGC TCAGTAGAAT TAACCAGGAA 
GCAAACCCTG GAAACATGGT TGAGAAATTA GGAGCTGATG CAAAAATTCT TTCAAATGTA 
ATCTCAAAAA GCACAAGACC AAATAGTCTT AATATTGGAA AACCGTCTTT AAGATCAAAG 
AGAGACAGTG TAGAAAAGGA ATCGAGTGAT GATGATACAC CTTTTGATGG TTCTAATTGT 
CTGACAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAATGAAACA 
GATATTTCAA AAGCGAGGTA CATTGTTCCA CAAAATCCCA AGAGGAGTCA ACGCTTGAAC 
AGCAGCTTTT CAATAAAACC TTTTGAAAAA ACTGATGTTG CCACAGGATT TGATCCCCTC 
TCACTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAGG AGGAGGAAGA GGAGGAGGAT 
GACAGCAAAA GTGTTTCAAC ACCATCTGCT AGGCGGGATT TAGCTGAAGA AATTGAGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGAACAC CAAGCATTGA TTTACAACGG 
GCCTGTGATG ATAAAGTCAC CACTAAGAAA AGTCCAACGT TGGTCAAGGC ATGCAGAAGA 
TCTAGCCTGC CTCCTAATTC TCCCAGACCA GTGAGACTTA CCAAATCCAA AAGTTACTCA 
AAAAATGAGG ACAGGCCAAG GGATAGACTC TGGTCTTCTC CAGCCTTCTC ACCTACCTGT 
CCATTTAGGG AAGAATCTCA AGATGCATTA ACCCATTCAT CACCTTCATT TAATTTAGAC 
ACACTACTAG TACCTAAACT AGATGTTCTG AGAAACAGTA TGTTCACTGC TGGAAAAGGA 
GTTGCCGAGA AAGCAAGCAA ATGGTATTCA AGATTCACCA TGTACACCAC ATCATCAAAG 
GATCAAAGTT CTGACCGTAC CAGTCTTTCT TCAGTGGGAG CCCAGGATTC TGAATCTACC 
TCTTTAACAG ATGAAGATGT CTGCCATGAG TTGGAAGGAG CCACTTCTCA GGAGAGCAGC 
GCCACTTCAG GGACTAAGGG AGTTGATTTC AGCAGAACAA GCCTGGAAGG CTCTGCCTCC 
TTAGAAGGAT CCTTATCCAA GTTTGCCTTA CCTGGGAAAT CAGAGGTGGC ATCTTCCTGC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTCCTTAT CTCAAGCTGC 
TCTCGGTGTA AAACCTGCGA TTGTCTGGTC CACGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACTACCTGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
CTGAATATAG AGATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTTTGAA GTCAAGCCCA 
TCTACAGAAA ATATACATTT TCCATCCTCC TTTTCAAGTC AGACAAGGCA GTCTTGCATC 
TCAACATCAA CCTCTGGTCT TGACACATCT CTTCTCTCTG TTCACGGGAG TTTTGATCTG 
AATAGCAAAT CTAAGCCGCT GGAAAATACA TGTGCCCGAA GCATTCAGAT CCCTGCTGAT 
AGATCAAAAG CAGCTATGTC AAAATGTCCG TTATTTCCAA TGGCCAGGAG TGTTAGTACT 
TATGGCACCT TGGATCAGGA AGACATTGGC GGGCAGAAGC TCGTTCCTAC AGGCAGCCTG 
CCAACCACCT TACAAGGCGC TGCGGATTCT TTAGGATTAG AATGGCACCT CCCAAGTCCA 
GATCCTATCA CTGTTCCGTA CCTTAGCCCA CTAGTGGTGT GGAAGGAACT TGAAAGCTTA 
CTGGAAAACG AAGGTGATCG TGCAATAACA GTGGCAGACT TCGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTGTG GTATTTTAGA CGTCTTGACT TACCCAGTAA TTTACCTGGA 
CTGATTCTTT CTTCTGAGCA TTGTAACAAG TATTCAAAGA TTCCTCGCCA CTGTATGTCT 
GAAGACAGTA AATATGTTTT AATACAGATG TTGTGGGACA ATATGAAATT ACATCAGGAT 
CCAGGACAGC CCTTGTACAT CCTCTGGAAT GCGCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAAG GTGATAATTC ATTTAACCAG 
GAACTGTTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG ACACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTAT TTCTCTCACT GGTGGCACTG GGAAGAGAAA ACATTGATAT AGATGCATTT 
GATAAAGAAT ATAAGATGGC ATATGATCGT CTCACACCCA GTCAAGTCAA GAGCACCCAC 
AACTGCGACA GGCCGCCAAG CACAGGGGTG ATGGAATGTC GGAAGACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853258.1
CDS	119..5647
Translation	MGNQYFSNSEACRIHKLRSGVIFRETFSNVVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_024997490.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997490.1

ORF Insert Sequence:

ATGGGAAACC AGTATTTCTC AAACTCTGAG GCTTGCAGAA TTCACAAATT GAGATCTGGC 
GTCATTTTCA GAGAGACTTT CAGTAATGTG GTTTTATATG ACTGGAAAGA AAGATTGAAA 
CAAGGGTGTG AAATTATCCA GAGTACTCCC TATGGACGCC CTGCAAATAT TAGCGGCAGT 
ACCTCATCAC AAAGAATTTA TATCACTTAC CGAAGAGCCT CTGAAAACAT GACTCAGAAT 
ACGTTGGCTG TTACAGACAT ATGTATTATT ATACCCAGTA AAGGAGAAAG TCCACCACAC 
ACATTCTGCA AAGTTGACAA GAATCTCAAT AACAGTATGT GGGGATCAGC AGTATACTTA 
TGTTATAAAA AATCAGTGGC AAAGACTAAC ACTATATCTT ACAAAGCTGG TCTAATTTGT 
AGATACCCTC AAGAAGATTA CGAGTCATTC TCACTCCCAG AATCTGTTCC CCTGTTTTGT 
TTACCAATGG GCGCAACTAT TGAATGTTGG CCATCAAATA GCAAATACCC CCTCCCTGTA 
TTTTCTACAT TCGTGTTAAC TGGAGCCTCA GCTGAAAAGG TCTATGGTGC TGCTATTCAG 
TTTTATGAAC CATACTCCGA GGAGAATCTC ACAGAAAAAC AGAGATTTCT TTTGGGTTTA 
GCATCATCAG CAAATGAGAA GTCTGATAGT TCCAAGACAA TTCACACTAA TAAATGCATC 
TGTCTTCTCT CTCACTGGCC TTTTTTTGAC GCATTCAGGA AGTTTTTGAC TTTTCTATAT 
CGTTATTCCA TCTCTGGACC TCACGTCCTT CCAATTGAGA AGCATATTTC TCATTTTATG 
CATAAAGTTC CTTTTCCATC TCCTCAGAGA CCACGGATTC TAGTTCAGTT ATCACCACAT 
GATAATCTGA TCCTCAGTCA GCCTGTTTCT TCACCTCTTC CACTAAGTGG TGGCAAGTTT 
TCAACACTGC TCCAGAATTT AGGCCCTGAA AATGCTGTGA CACTCCTGGT GTTTGCAGTA 
ACAGAACATA AAATTCTCAT TCATTCCTTA CGGCCTTCCG TGCTTACCAG TGTGACAGAA 
GCATTAGTGT CTATGATTTT CCCTTTCCAC TGGCCATGCC CCTATGTTCC TCTCTGCCCA 
CTGGCTTTAG CAGATGTCTT GAGTGCACCA TGTCCATTCA TAGTAGGAAT TGATTCAAGA 
TACTTTGATC TCTATGACCC TCCACCAGAT GTCAGTTGTG TTGACCTAGA TACCAACACC 
ATTTCTCAAA CTGGTGACAA GAAAAATGTA GCCTGGAAGA TACTTCCAAA GAAACCATGT 
AAAAATCTGA TGAACACACT GAATAATTTG CATCAACAAC TGGCAAAATT ACAGCAGAGA 
CCAAGAGATG ATGGACTTAT GGACTTAGCA ATGAATGATT ATGACTTTAA TTCTGGAAAG 
AGGTTGCACA TGATAGACTT GGAAATCCAA GAGGCATTTT TGTGTTTCAT GGCCTCTATT 
TTAAAAGGTT ATAGATCATA CTTAAGACCA ATAACTCAAG CCCCATCTGA GACAGCCACA 
GATGCAACTT CTCTTTTTGC TCTACAAGCT TTCTTAAGAA GTCGGGACCG GTCACATCAG 
AAATTCTATA ACATGATGAC CAAAACACAA ATGTTTATTC GCTTCATTGA GGAATGTTCT 
TTCGTCAGTG ATAAAGATGC AAGTCTGGCG TTTTTTGATG ATTGTGTAGA TAAAGTGGAT 
ATGGACAAGA GTGGTGAAGT GCGACTTATA GAATTGGATG AATCTTTCAA AAGTGAACAT 
ACTGTTTTTG TAACACCTCC TGAGATCCCC CATCTACCCA ATGGTGAAGA ACCCCCTTTA 
CAGTACAGCT ATAATGGATT TCCAGTTCTT AGGAACAATT TATTTGAGAG ACCTGAAGGA 
TTTCTGCAAA CACGAAAGAG CAAATTGCCT TCAAAATCAA GTAGTCCTAG TAGCCCTTCT 
CCCATGTTCA GAAGAACGAA ACAGGAAATT AAATCAGCCC ATAAAATTGC CAAGAGATAT 
TCTTCCATTC CTCAGATGTG GTCTAGGTGT CTACTGCGTC ACTGTTATGG ACTCTGGTTT 
ATTTGTCTCC CAGCTTATGT GAAAGTATGC CATTCAAAAG TCAGGGCTCT GAAAACAGCA 
TATGATGTAC TCAAAAAAAT GCAGTCAAAG AAGATGGATC CACCTGATGA GGTGTGCTAC 
CGCATTCTCA TGCAGCTCTG CGGACAGTAT GATCAGCCAG TGCTTGCAGT TCGAGTGCTT 
TTTGAAATGC AGAAAGCTGG TATTGACCCA AATGCTATTA CTTATGGCTA TTATAATAAG 
GCTGTTTTGG AAAGTACTTG GCCTTCAAGG AGTCGTAGTG GATATTTTCT TTGGACAAAA 
GTGAGAAATG TTGTTTTAGG AGTAGCACAG TTCAAAAGAG CTTTAAAGAA GCATGCGAAT 
TTATCTCAAA CAGCTCTCTC AGCAGATGGC AGTGACCTGG ACGCTGTTAG CCATGGCAGC 
ATGGATAGTG GCCATGGGAC ACACGCTGTG GAGCAGGCGC CGTGTAGTAC AGGTTCAATC 
AAAGCGTATG CTTCCGATGA TAGATCTAGT ACAGGTGGCC AGTCTGACCT TGGATATAAT 
TCGTTATCTA AGGATGAAGT TAGAAGAGGG GATACATCCA CTGAGGACAT TCAAGAAGAA 
AAAGATAAGA AAGGGAGTGA TTCTAGTTCC TTGTCAGAGT GCGAGAGTGC AAAGGGAAGT 
GCCGACTGTC TTCCTAAGCT CGGTTATCAA AGTTCTTCCA GTATAGTTCG CCTCAATGGT 
ACAAGTAACA GTGCTGATAA AATATCAGGA GAAAGCACAG AAAGCACATC TGAGCCGCTC 
TTAATATCGT CTTTTGAGGA TGCAAATGAA ACAGGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAGCCTGAT TTGCAGCAGC AAATGTCCTG GGTAAATCGA 
AGCCGCAACC TTAGTGGAGG AGTGCTGATG GGACTTATGC TCAGTAGAAT TAACCAGGAA 
GCAAACCCTG GAAACATGGT TGAGAAATTA GGAGCTGATG CAAAAATTCT TTCAAATGTA 
ATCTCAAAAA GCACAAGACC AAATAGTCTT AATATTGGAA AACCGTCTTT AAGATCAAAG 
AGAGACAGTG TAGAAAAGGA ATCGAGTGAT GATGATACAC CTTTTGATGG TTCTAATTGT 
CTGACAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAATGAAACA 
GATATTTCAA AAGCGAGGTA CATTGTTCCA CAAAATCCCA AGAGGAGTCA ACGCTTGAAC 
AGCAGCTTTT CAATAAAACC TTTTGAAAAA ACTGATGTTG CCACAGGATT TGATCCCCTC 
TCACTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAGG AGGAGGAAGA GGAGGAGGAT 
GACAGCAAAA GTGTTTCAAC ACCATCTGCT AGGCGGGATT TAGCTGAAGA AATTGAGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGAACAC CAAGCATTGA TTTACAACGG 
GCCTGTGATG ATAAAGTCAC CACTAAGAAA AGTCCAACGT TGGTCAAGGC ATGCAGAAGA 
TCTAGCCTGC CTCCTAATTC TCCCAGACCA GTGAGACTTA CCAAATCCAA AAGTTACTCA 
AAAAATGAGG ACAGGCCAAG GGATAGACTC TGGTCTTCTC CAGCCTTCTC ACCTACCTGT 
CCATTTAGGG AAGAATCTCA AGATGCATTA ACCCATTCAT CACCTTCATT TAATTTAGAC 
ACACTACTAG TACCTAAACT AGATGTTCTG AGAAACAGTA TGTTCACTGC TGGAAAAGGA 
GTTGCCGAGA AAGCAAGCAA ATGGTATTCA AGATTCACCA TGTACACCAC ATCATCAAAG 
GATCAAAGTT CTGACCGTAC CAGTCTTTCT TCAGTGGGAG CCCAGGATTC TGAATCTACC 
TCTTTAACAG ATGAAGATGT CTGCCATGAG TTGGAAGGAG CCACTTCTCA GGAGAGCAGC 
GCCACTTCAG GGACTAAGGG AGTTGATTTC AGCAGAACAA GCCTGGAAGG CTCTGCCTCC 
TTAGAAGGAT CCTTATCCAA GTTTGCCTTA CCTGGGAAAT CAGAGGTGGC ATCTTCCTGC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTCCTTAT CTCAAGCTGC 
TCTCGGTGTA AAACCTGCGA TTGTCTGGTC CACGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACTACCTGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
CTGAATATAG AGATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTTTGAA GTCAAGCCCA 
TCTACAGAAA ATATACATTT TCCATCCTCC TTTTCAAGTC AGACAAGGCA GTCTTGCATC 
TCAACATCAA CCTCTGGTCT TGACACATCT CTTCTCTCTG TTCACGGGAG TTTTGATCTG 
AATAGCAAAT CTAAGCCGCT GGAAAATACA TGTGCCCGAA GCATTCAGAT CCCTGCTGAT 
AGATCAAAAG CAGCTATGTC AAAATGTCCG TTATTTCCAA TGGCCAGGAG TGTTAGTACT 
TATGGCACCT TGGATCAGGA AGACATTGGC GGGCAGAAGC TCGTTCCTAC AGGCAGCCTG 
CCAACCACCT TACAAGGCGC TGCGGATTCT TTAGGATTAG AATGGCACCT CCCAAGTCCA 
GATCCTATCA CTGTTCCGTA CCTTAGCCCA CTAGTGGTGT GGAAGGAACT TGAAAGCTTA 
CTGGAAAACG AAGGTGATCG TGCAATAACA GTGGCAGACT TCGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTGTG GTATTTTAGA CGTCTTGACT TACCCAGTAA TTTACCTGGA 
CTGATTCTTT CTTCTGAGCA TTGTAACAAG TATTCAAAGA TTCCTCGCCA CTGTATGTCT 
GAAGACAGTA AATATGTTTT AATACAGATG TTGTGGGACA ATATGAAATT ACATCAGGAT 
CCAGGACAGC CCTTGTACAT CCTCTGGAAT GCGCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAAG GTGATAATTC ATTTAACCAG 
GAACTGTTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG ACACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTAT TTCTCTCACT GGTGGCACTG GGAAGAGAAA ACATTGATAT AGATGCATTT 
GATAAAGAAT ATAAGATGGC ATATGATCGT CTCACACCCA GTCAAGTCAA GAGCACCCAC 
AACTGCGACA GGCCGCCAAG CACAGGGGTG ATGGAATGTC GGAAGACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001205525.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192454.2 Bos taurus DENN domain containing 4A (DENND4A), mRNA.

Price & Availability↑

CloneID	OBa22956
Clone ID Related Accession (Same CDS sequence)	XM_005211325.3 , NM_001205525.3 , XM_005211325.4 , NM_001205525.2
Accession Version	NM_001205525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Dec 20, 2018 this sequence version replaced NM_001205525.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.10154.1, SRR5571260.45650.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192454.2
CDS	1..5721
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001205525.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205525.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001205525.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192454.2 Bos taurus DENN domain containing 4A (DENND4A), mRNA.

Price & Availability↑

CloneID	OBa22956
Clone ID Related Accession (Same CDS sequence)	XM_005211325.3 , NM_001205525.3 , XM_005211325.4 , NM_001205525.2
Accession Version	NM_001205525.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02027871.1 and DAAA02027870.1. On Jun 30, 2017 this sequence version replaced NM_001205525.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192454.2
CDS	1..5721
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001205525.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205525.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001205525.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192454.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), mRNA.

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CloneID	OBa39499
Clone ID Related Accession (Same CDS sequence)	NM_001205525.1
Accession Version	NM_001205525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	C-myc promoter-binding protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03047787.1, AAFC03035815.1, AAFC03035814.1, AAFC03085564.1, AAFC03035816.1 and AAFC03085566.1. On Apr 20, 2011 this sequence version replaced XM_002700495.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTACGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192454.1
CDS	1..5721
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLRPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001205525.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205525.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTACGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211320.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211377.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OBa22953
Clone ID Related Accession (Same CDS sequence)	XM_005211320.3 , XM_005211320.4 , XM_005211319.3 , XM_015473032.1 , XM_005211319.4
Accession Version	XM_005211320.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211377.1
CDS	271..6021
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211320.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211320.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211320.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211377.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OBa22953
Clone ID Related Accession (Same CDS sequence)	XM_005211320.3 , XM_005211320.4 , XM_005211319.3 , XM_015473032.1 , XM_005211319.4
Accession Version	XM_005211320.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211320.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211377.1
CDS	208..5958
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211320.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211320.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211319.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211376.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OBa22953
Clone ID Related Accession (Same CDS sequence)	XM_005211320.3 , XM_005211320.4 , XM_005211319.3 , XM_015473032.1 , XM_005211319.4
Accession Version	XM_005211319.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211376.1
CDS	347..6097
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211319.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211319.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_015473032.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328518.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OBa22953
Clone ID Related Accession (Same CDS sequence)	XM_005211320.3 , XM_005211320.4 , XM_005211319.3 , XM_015473032.1 , XM_005211319.4
Accession Version	XM_015473032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328518.1
CDS	118..5868
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_015473032.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473032.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211319.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211376.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OBa22953
Clone ID Related Accession (Same CDS sequence)	XM_005211320.3 , XM_005211320.4 , XM_005211319.3 , XM_015473032.1 , XM_005211319.4
Accession Version	XM_005211319.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211319.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211376.1
CDS	285..6035
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211319.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211319.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAG TCAAAATCGT ACTCTTTTGT TTGAGACCCA AAAGTATCCA 
ATGGTCCATT TATTGCAAAA AGGTGATAAT TCATTTAACC AGGAACTGTT GAAAAATATG 
GTAAAAAGCA TTAAAATGAA TGATGTCTAT GGACCAATGA GTCAGATTTT AGACACACTG 
AATAAATGTC CTCATTTTAA AAGACAGAGG AGTTTATATA GAGAAATACT ATTTCTCTCA 
CTGGTGGCAC TGGGAAGAGA AAACATTGAT ATAGATGCAT TTGATAAAGA ATATAAGATG 
GCATATGATC GTCTCACACC CAGTCAAGTC AAGAGCACCC ACAACTGCGA CAGGCCGCCA 
AGCACAGGGG TGATGGAATG TCGGAAGACC TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211323.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211380.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OBa22954
Clone ID Related Accession (Same CDS sequence)	XM_005211323.4 , XM_005211323.3
Accession Version	XM_005211323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211323.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACAGTCA AAATCGTACT CTTTTGTTTG AGACCCAAAA GTATCCAATG 
GTCCATTTAT TGCAAAAAGG TGATAATTCA TTTAACCAGG AACTGTTGAA AAATATGGTA 
AAAAGCATTA AAATGAATGA TGTCTATGGA CCAATGAGTC AGATTTTAGA CACACTGAAT 
AAATGTCCTC ATTTTAAAAG ACAGAGGAGT TTATATAGAG AAATACTATT TCTCTCACTG 
GTGGCACTGG GAAGAGAAAA CATTGATATA GATGCATTTG ATAAAGAATA TAAGATGGCA 
TATGATCGTC TCACACCCAG TCAAGTCAAG AGCACCCACA ACTGCGACAG GCCGCCAAGC 
ACAGGGGTGA TGGAATGTCG GAAGACCTTT GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211380.1
CDS	285..6032
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211323.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211323.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACAGTCA AAATCGTACT CTTTTGTTTG AGACCCAAAA GTATCCAATG 
GTCCATTTAT TGCAAAAAGG TGATAATTCA TTTAACCAGG AACTGTTGAA AAATATGGTA 
AAAAGCATTA AAATGAATGA TGTCTATGGA CCAATGAGTC AGATTTTAGA CACACTGAAT 
AAATGTCCTC ATTTTAAAAG ACAGAGGAGT TTATATAGAG AAATACTATT TCTCTCACTG 
GTGGCACTGG GAAGAGAAAA CATTGATATA GATGCATTTG ATAAAGAATA TAAGATGGCA 
TATGATCGTC TCACACCCAG TCAAGTCAAG AGCACCCACA ACTGCGACAG GCCGCCAAGC 
ACAGGGGTGA TGGAATGTCG GAAGACCTTT GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211323.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211380.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OBa22954
Clone ID Related Accession (Same CDS sequence)	XM_005211323.4 , XM_005211323.3
Accession Version	XM_005211323.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACAGTCA AAATCGTACT CTTTTGTTTG AGACCCAAAA GTATCCAATG 
GTCCATTTAT TGCAAAAAGG TGATAATTCA TTTAACCAGG AACTGTTGAA AAATATGGTA 
AAAAGCATTA AAATGAATGA TGTCTATGGA CCAATGAGTC AGATTTTAGA CACACTGAAT 
AAATGTCCTC ATTTTAAAAG ACAGAGGAGT TTATATAGAG AAATACTATT TCTCTCACTG 
GTGGCACTGG GAAGAGAAAA CATTGATATA GATGCATTTG ATAAAGAATA TAAGATGGCA 
TATGATCGTC TCACACCCAG TCAAGTCAAG AGCACCCACA ACTGCGACAG GCCGCCAAGC 
ACAGGGGTGA TGGAATGTCG GAAGACCTTT GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211380.1
CDS	348..6095
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211323.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211323.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACAGTCA AAATCGTACT CTTTTGTTTG AGACCCAAAA GTATCCAATG 
GTCCATTTAT TGCAAAAAGG TGATAATTCA TTTAACCAGG AACTGTTGAA AAATATGGTA 
AAAAGCATTA AAATGAATGA TGTCTATGGA CCAATGAGTC AGATTTTAGA CACACTGAAT 
AAATGTCCTC ATTTTAAAAG ACAGAGGAGT TTATATAGAG AAATACTATT TCTCTCACTG 
GTGGCACTGG GAAGAGAAAA CATTGATATA GATGCATTTG ATAAAGAATA TAAGATGGCA 
TATGATCGTC TCACACCCAG TCAAGTCAAG AGCACCCACA ACTGCGACAG GCCGCCAAGC 
ACAGGGGTGA TGGAATGTCG GAAGACCTTT GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211324.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211381.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OBa22955
Clone ID Related Accession (Same CDS sequence)	XM_005211324.4 , XM_005211324.3
Accession Version	XM_005211324.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211324.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAC CCAAAAGTAT CCAATGGTCC ATTTATTGCA AAAAGGTGAT 
AATTCATTTA ACCAGGAACT GTTGAAAAAT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGACACA CTGAATAAAT GTCCTCATTT TAAAAGACAG 
AGGAGTTTAT ATAGAGAAAT ACTATTTCTC TCACTGGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CATTTGATAA AGAATATAAG ATGGCATATG ATCGTCTCAC ACCCAGTCAA 
GTCAAGAGCA CCCACAACTG CGACAGGCCG CCAAGCACAG GGGTGATGGA ATGTCGGAAG 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211381.1
CDS	373..6096
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211324.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211324.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAC CCAAAAGTAT CCAATGGTCC ATTTATTGCA AAAAGGTGAT 
AATTCATTTA ACCAGGAACT GTTGAAAAAT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGACACA CTGAATAAAT GTCCTCATTT TAAAAGACAG 
AGGAGTTTAT ATAGAGAAAT ACTATTTCTC TCACTGGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CATTTGATAA AGAATATAAG ATGGCATATG ATCGTCTCAC ACCCAGTCAA 
GTCAAGAGCA CCCACAACTG CGACAGGCCG CCAAGCACAG GGGTGATGGA ATGTCGGAAG 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211324.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211381.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OBa22955
Clone ID Related Accession (Same CDS sequence)	XM_005211324.4 , XM_005211324.3
Accession Version	XM_005211324.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAC CCAAAAGTAT CCAATGGTCC ATTTATTGCA AAAAGGTGAT 
AATTCATTTA ACCAGGAACT GTTGAAAAAT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGACACA CTGAATAAAT GTCCTCATTT TAAAAGACAG 
AGGAGTTTAT ATAGAGAAAT ACTATTTCTC TCACTGGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CATTTGATAA AGAATATAAG ATGGCATATG ATCGTCTCAC ACCCAGTCAA 
GTCAAGAGCA CCCACAACTG CGACAGGCCG CCAAGCACAG GGGTGATGGA ATGTCGGAAG 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211381.1
CDS	346..6069
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSADGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211324.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211324.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGCAGATG GCAGTGACCT GGACGCTGTT AGCCATGGCA GCATGGATAG TGGCCATGGG 
ACACACGCTG TGGAGCAGGC GCCGTGTAGT ACAGGTTCAA TCAAAGCGTA TGCTTCCGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAC CTTGGATATA ATTCGTTATC TAAGGATGAA 
GTTAGAAGAG GGGATACATC CACTGAGGAC ATTCAAGAAG AAAAAGATAA GAAAGGGAGT 
GATTCTAGTT CCTTGTCAGA GTGCGAGAGT GCAAAGGGAA GTGCCGACTG TCTTCCTAAG 
CTCGGTTATC AAAGTTCTTC CAGTATAGTT CGCCTCAATG GTACAAGTAA CAGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTGAGCCGC TCTTAATATC GTCTTTTGAG 
GATGCAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAGCCTG ATTTGCAGCA GCAAATGTCC TGGGTAAATC GAAGCCGCAA CCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTCAGTAGA ATTAACCAGG AAGCAAACCC TGGAAACATG 
GTTGAGAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TAATCTCAAA AAGCACAAGA 
CCAAATAGTC TTAATATTGG AAAACCGTCT TTAAGATCAA AGAGAGACAG TGTAGAAAAG 
GAATCGAGTG ATGATGATAC ACCTTTTGAT GGTTCTAATT GTCTGACAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATATTTC AAAAGCGAGG 
TACATTGTTC CACAAAATCC CAAGAGGAGT CAACGCTTGA ACAGCAGCTT TTCAATAAAA 
CCTTTTGAAA AAACTGATGT TGCCACAGGA TTTGATCCCC TCTCACTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGAGGAGG ATGACAGCAA AAGTGTTTCA 
ACACCATCTG CTAGGCGGGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGAAC ACCAAGCATT GATTTACAAC GGGCCTGTGA TGATAAAGTC 
ACCACTAAGA AAAGTCCAAC GTTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCCAGAC CAGTGAGACT TACCAAATCC AAAAGTTACT CAAAAAATGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACCT GTCCATTTAG GGAAGAATCT 
CAAGATGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ACACACTACT AGTACCTAAA 
CTAGATGTTC TGAGAAACAG TATGTTCACT GCTGGAAAAG GAGTTGCCGA GAAAGCAAGC 
AAATGGTATT CAAGATTCAC CATGTACACC ACATCATCAA AGGATCAAAG TTCTGACCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTCTGCCATG AGTTGGAAGG AGCCACTTCT CAGGAGAGCA GCGCCACTTC AGGGACTAAG 
GGAGTTGATT TCAGCAGAAC AAGCCTGGAA GGCTCTGCCT CCTTAGAAGG ATCCTTATCC 
AAGTTTGCCT TACCTGGGAA ATCAGAGGTG GCATCTTCCT GCAACACGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTCCTT ATCTCAAGCT GCTCTCGGTG TAAAACCTGC 
GATTGTCTGG TCCACGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA CTCAAATCTC 
AATACTACCT GCCCGTTTTG TGGCAATCTT TTCTTGCCCT TTCTGAATAT AGAGATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTTTG AAGTCAAGCC CATCTACAGA AAATATACAT 
TTTCCATCCT CCTTTTCAAG TCAGACAAGG CAGTCTTGCA TCTCAACATC AACCTCTGGT 
CTTGACACAT CTCTTCTCTC TGTTCACGGG AGTTTTGATC TGAATAGCAA ATCTAAGCCG 
CTGGAAAATA CATGTGCCCG AAGCATTCAG ATCCCTGCTG ATAGATCAAA AGCAGCTATG 
TCAAAATGTC CGTTATTTCC AATGGCCAGG AGTGTTAGTA CTTATGGCAC CTTGGATCAG 
GAAGACATTG GCGGGCAGAA GCTCGTTCCT ACAGGCAGCC TGCCAACCAC CTTACAAGGC 
GCTGCGGATT CTTTAGGATT AGAATGGCAC CTCCCAAGTC CAGATCCTAT CACTGTTCCG 
TACCTTAGCC CACTAGTGGT GTGGAAGGAA CTTGAAAGCT TACTGGAAAA CGAAGGTGAT 
CGTGCAATAA CAGTGGCAGA CTTCGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTG 
TGGTATTTTA GACGTCTTGA CTTACCCAGT AATTTACCTG GACTGATTCT TTCTTCTGAG 
CATTGTAACA AGTATTCAAA GATTCCTCGC CACTGTATGT CTGAAGACAG TAAATATGTT 
TTAATACAGA TGTTGTGGGA CAATATGAAA TTACATCAGG ATCCAGGACA GCCCTTGTAC 
ATCCTCTGGA ATGCGCACAC CCAAAAGTAT CCAATGGTCC ATTTATTGCA AAAAGGTGAT 
AATTCATTTA ACCAGGAACT GTTGAAAAAT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGACACA CTGAATAAAT GTCCTCATTT TAAAAGACAG 
AGGAGTTTAT ATAGAGAAAT ACTATTTCTC TCACTGGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CATTTGATAA AGAATATAAG ATGGCATATG ATCGTCTCAC ACCCAGTCAA 
GTCAAGAGCA CCCACAACTG CGACAGGCCG CCAAGCACAG GGGTGATGGA ATGTCGGAAG 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211325.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211382.1 Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OBa22956
Clone ID Related Accession (Same CDS sequence)	XM_005211325.3 , NM_001205525.3 , XM_005211325.4 , NM_001205525.2
Accession Version	XM_005211325.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211382.1
CDS	347..6067
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211325.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211325.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005211325.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211382.1 Bos taurus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OBa22956
Clone ID Related Accession (Same CDS sequence)	XM_005211325.3 , NM_001205525.3 , XM_005211325.4 , NM_001205525.2
Accession Version	XM_005211325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211382.1
CDS	380..6100
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVARPKEPITDVSVIIKSLGEEVPRDYTCIDITPTGLSADLNNGSLVGPQIFLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRFLLGLASSANEKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKSKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHANLSQTALSDGSDLDAVSHGSMDSGHGTHAVEQAPCSTGSIKAYASDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDSSSLSECESAKGSADCLPKLGYQSSSSIVRLNGTSNSADKISGESTESTSEPLLISSFEDANETGNIQSRCFRKRHKSDNEPDLQQQMSWVNRSRNLSGGVLMGLMLSRINQEANPGNMVEKLGADAKILSNVISKSTRPNSLNIGKPSLRSKRDSVEKESSDDDTPFDGSNCLTDKVDSPVIFDLEDLDNETDISKARYIVPQNPKRSQRLNSSFSIKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEEEDDSKSVSTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKVTTKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYSKNEDRPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSQESSATSGTKGVDFSRTSLEGSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCKTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENIHFPSSFSSQTRQSCISTSTSGLDTSLLSVHGSFDLNSKSKPLENTCARSIQIPADRSKAAMSKCPLFPMARSVSTYGTLDQEDIGGQKLVPTGSLPTTLQGAADSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDRAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILDTLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005211325.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211325.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCCAGA 
CCAAAAGAGC CTATCACAGA TGTTTCAGTT ATTATTAAAT CTCTCGGGGA GGAAGTGCCA 
CGGGATTACA CATGTATTGA TATTACCCCA ACGGGACTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCGCAGAT TTTCCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTG 
ACGGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAAGGGTG TGAAATTATC 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGCGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTTACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGTCCACCAC ACACATTCTG CAAAGTTGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC TTACAAAGCT GGTCTAATTT GTAGATACCC TCAAGAAGAT 
TACGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTGTTTT GTTTACCAAT GGGCGCAACT 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCCCTCCCTG TATTTTCTAC ATTCGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCC 
GAGGAGAATC TCACAGAAAA ACAGAGATTT CTTTTGGGTT TAGCATCATC AGCAAATGAG 
AAGTCTGATA GTTCCAAGAC AATTCACACT AATAAATGCA TCTGTCTTCT CTCTCACTGG 
CCTTTTTTTG ACGCATTCAG GAAGTTTTTG ACTTTTCTAT ATCGTTATTC CATCTCTGGA 
CCTCACGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GACCACGGAT TCTAGTTCAG TTATCACCAC ATGATAATCT GATCCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTCCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTCCTG GTGTTTGCAG TAACAGAACA TAAAATTCTC 
ATTCATTCCT TACGGCCTTC CGTGCTTACC AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCATG CCCCTATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTCAGTTG TGTTGACCTA GATACCAACA CCATTTCTCA AACTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCATCAACA ACTGGCAAAA TTACAGCAGA GACCAAGAGA TGATGGACTT 
ATGGACTTAG CAATGAATGA TTATGACTTT AATTCTGGAA AGAGGTTGCA CATGATAGAC 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACTCA AGCCCCATCT GAGACAGCCA CAGATGCAAC TTCTCTTTTT 
GCTCTACAAG CTTTCTTAAG AAGTCGGGAC CGGTCACATC AGAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTCGTCAG TGATAAAGAT 
GCAAGTCTGG CGTTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
GTGCGACTTA TAGAATTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCT 
CCTGAGATCC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAACAA TTTATTTGAG AGACCTGAAG GATTTCTGCA AACACGAAAG 
AGCAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACG 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGAT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTCTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GCCATTCAAA AGTCAGGGCT CTGAAAACAG CATATGATGT ACTCAAAAAA 
ATGCAGTCAA AGAAGATGGA TCCACCTGAT GAGGTGTGCT ACCGCATTCT CATGCAGCTC 
TGCGGACAGT ATGATCAGCC AGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CAAATGCTAT TACTTATGGC TATTATAATA AGGCTGTTTT GGAAAGTACT 
TGGCCTTCAA GGAGTCGTAG TGGATATTTT CTTTGGACAA AAGTGAGAAA TGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAGCATGCGA ATTTATCTCA AACAGCTCTC 
TCAGATGGCA GTGACCTGGA CGCTGTTAGC CATGGCAGCA TGGATAGTGG CCATGGGACA 
CACGCTGTGG AGCAGGCGCC GTGTAGTACA GGTTCAATCA AAGCGTATGC TTCCGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGACCTT GGATATAATT CGTTATCTAA GGATGAAGTT 
AGAAGAGGGG ATACATCCAC TGAGGACATT CAAGAAGAAA AAGATAAGAA AGGGAGTGAT 
TCTAGTTCCT TGTCAGAGTG CGAGAGTGCA AAGGGAAGTG CCGACTGTCT TCCTAAGCTC 
GGTTATCAAA GTTCTTCCAG TATAGTTCGC CTCAATGGTA CAAGTAACAG TGCTGATAAA 
ATATCAGGAG AAAGCACAGA AAGCACATCT GAGCCGCTCT TAATATCGTC TTTTGAGGAT 
GCAAATGAAA CAGGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAGCCTGATT TGCAGCAGCA AATGTCCTGG GTAAATCGAA GCCGCAACCT TAGTGGAGGA 
GTGCTGATGG GACTTATGCT CAGTAGAATT AACCAGGAAG CAAACCCTGG AAACATGGTT 
GAGAAATTAG GAGCTGATGC AAAAATTCTT TCAAATGTAA TCTCAAAAAG CACAAGACCA 
AATAGTCTTA ATATTGGAAA ACCGTCTTTA AGATCAAAGA GAGACAGTGT AGAAAAGGAA 
TCGAGTGATG ATGATACACC TTTTGATGGT TCTAATTGTC TGACAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AATGAAACAG ATATTTCAAA AGCGAGGTAC 
ATTGTTCCAC AAAATCCCAA GAGGAGTCAA CGCTTGAACA GCAGCTTTTC AATAAAACCT 
TTTGAAAAAA CTGATGTTGC CACAGGATTT GATCCCCTCT CACTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAGGA GGAGGAAGAG GAGGAGGATG ACAGCAAAAG TGTTTCAACA 
CCATCTGCTA GGCGGGATTT AGCTGAAGAA ATTGAGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGAACACC AAGCATTGAT TTACAACGGG CCTGTGATGA TAAAGTCACC 
ACTAAGAAAA GTCCAACGTT GGTCAAGGCA TGCAGAAGAT CTAGCCTGCC TCCTAATTCT 
CCCAGACCAG TGAGACTTAC CAAATCCAAA AGTTACTCAA AAAATGAGGA CAGGCCAAGG 
GATAGACTCT GGTCTTCTCC AGCCTTCTCA CCTACCTGTC CATTTAGGGA AGAATCTCAA 
GATGCATTAA CCCATTCATC ACCTTCATTT AATTTAGACA CACTACTAGT ACCTAAACTA 
GATGTTCTGA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCCGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCAAAGG ATCAAAGTTC TGACCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGAGC CACTTCTCAG GAGAGCAGCG CCACTTCAGG GACTAAGGGA 
GTTGATTTCA GCAGAACAAG CCTGGAAGGC TCTGCCTCCT TAGAAGGATC CTTATCCAAG 
TTTGCCTTAC CTGGGAAATC AGAGGTGGCA TCTTCCTGCA ACACGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTCCTTATC TCAAGCTGCT CTCGGTGTAA AACCTGCGAT 
TGTCTGGTCC ACGATGAGGA AATCATGGCT GGCTGGACAG CAGATGACTC AAATCTCAAT 
ACTACCTGCC CGTTTTGTGG CAATCTTTTC TTGCCCTTTC TGAATATAGA GATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTTTGAAG TCAAGCCCAT CTACAGAAAA TATACATTTT 
CCATCCTCCT TTTCAAGTCA GACAAGGCAG TCTTGCATCT CAACATCAAC CTCTGGTCTT 
GACACATCTC TTCTCTCTGT TCACGGGAGT TTTGATCTGA ATAGCAAATC TAAGCCGCTG 
GAAAATACAT GTGCCCGAAG CATTCAGATC CCTGCTGATA GATCAAAAGC AGCTATGTCA 
AAATGTCCGT TATTTCCAAT GGCCAGGAGT GTTAGTACTT ATGGCACCTT GGATCAGGAA 
GACATTGGCG GGCAGAAGCT CGTTCCTACA GGCAGCCTGC CAACCACCTT ACAAGGCGCT 
GCGGATTCTT TAGGATTAGA ATGGCACCTC CCAAGTCCAG ATCCTATCAC TGTTCCGTAC 
CTTAGCCCAC TAGTGGTGTG GAAGGAACTT GAAAGCTTAC TGGAAAACGA AGGTGATCGT 
GCAATAACAG TGGCAGACTT CGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTGTGG 
TATTTTAGAC GTCTTGACTT ACCCAGTAAT TTACCTGGAC TGATTCTTTC TTCTGAGCAT 
TGTAACAAGT ATTCAAAGAT TCCTCGCCAC TGTATGTCTG AAGACAGTAA ATATGTTTTA 
ATACAGATGT TGTGGGACAA TATGAAATTA CATCAGGATC CAGGACAGCC CTTGTACATC 
CTCTGGAATG CGCACACCCA AAAGTATCCA ATGGTCCATT TATTGCAAAA AGGTGATAAT 
TCATTTAACC AGGAACTGTT GAAAAATATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGACACACTG AATAAATGTC CTCATTTTAA AAGACAGAGG 
AGTTTATATA GAGAAATACT ATTTCTCTCA CTGGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCAT TTGATAAAGA ATATAAGATG GCATATGATC GTCTCACACC CAGTCAAGTC 
AAGAGCACCC ACAACTGCGA CAGGCCGCCA AGCACAGGGG TGATGGAATG TCGGAAGACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DENND4A
Entrez Gene ID	514906
Full Name	DENN domain containing 4A
General protein information	Preferred Names DENN domain containing 4A Names C-myc promoter-binding protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	DENND4A	NP_001138295.1
Bos taurus (cattle)	DENND4A	NP_001192454.1
Gallus gallus (chicken)	DENND4A	XP_413911.4
Drosophila melanogaster (fruit fly)	Crag	NP_727281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001102	XP_322065.3
Pan troglodytes (chimpanzee)	DENND4A	XP_523102.2
	DENND4A	XP_001109950.1
Rattus norvegicus (Norway rat)	Dennd4a	XP_006243364.1
Mus musculus (house mouse)	Dennd4a	NP_001156389.1
Canis lupus familiaris (dog)	DENND4A	XP_005638584.1
Danio rerio (zebrafish)	dennd4a	NP_001074458.1
Xenopus tropicalis (tropical clawed frog)	dennd4a	XP_004912733.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DENND4A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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