POLA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLA2
Entrez Gene ID	514793
Full Name	DNA polymerase alpha 2, accessory subunit
General protein information	Preferred Names DNA polymerase alpha 2, accessory subunit Names DNA polymerase alpha subunit B DNA polymerase alpha 70 kDa subunit polymerase (DNA directed), alpha 2 (70kD subunit) polymerase (DNA directed), alpha 2, accessory subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005227073.3	XP_005227130.1	DNA polymerase alpha subunit B isoform X1
XM_005227073.4	XP_005227130.1	DNA polymerase alpha subunit B isoform X1
NM_001024542.2	NP_001019713.2	DNA polymerase alpha subunit B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pola2	NP_955875.1
Drosophila melanogaster (fruit fly)	DNApol-alpha73	NP_733206.3
Homo sapiens (human)	POLA2	NP_002680.2
	POLA2	XP_001113977.1
Bos taurus (cattle)	POLA2	NP_001019713.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004392	XP_313675.5
Pan troglodytes (chimpanzee)	POLA2	XP_001169495.1
Mus musculus (house mouse)	Pola2	NP_032919.2
Gallus gallus (chicken)	LOC100859759	XP_003643474.2
Oryza sativa (rice)	Os12g0242900	NP_001066483.1
Rattus norvegicus (Norway rat)	Pola2	NP_445932.2
Arabidopsis thaliana (thale cress)	POLA2	NP_176930.2
Canis lupus familiaris (dog)	POLA2	XP_533234.2
Xenopus tropicalis (tropical clawed frog)	pola2	NP_001005060.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44250	NM_001024542.2 Latest version!	Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa34791	XM_005227073.4 Latest version!	Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa34791	XM_005227073.3	Bos taurus polymerase (DNA directed), alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLA2 ( NM_001024542.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019713.2 Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), mRNA.

Price & Availability↑

CloneID	OBa44250
Clone ID Related Accession (Same CDS sequence)	NM_001024542.2
Accession Version	NM_001024542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha subunit B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW632685.1, BT021784.1, BF040752.1 and DT828048.1. On Aug 11, 2009 this sequence version replaced NM_001024542.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACGTA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGACATCC TCGGCTGCGT CTGTGTGAAC 
CCGGGGCGCC TCACCAAAGG CCAGGTGGGG GGCACCTTCG GCCGACTCTA CCTCAGGAGG 
CAGACCACAG GCGGTGAGGG GCGGCAGAGT CCGTGTGCTG CCGCCCAGGT GGTCAGGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019713.2
CDS	350..2152
Translation	MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLETLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGSHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKDILGCVCVNPGRLTKGQVGGTFGRLYLRRQTTGGEGRQSPCAAAQVVRI

Target ORF information:

RefSeq Version	NM_001024542.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024542.2

ORF Insert Sequence:

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACGTA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGACATCC TCGGCTGCGT CTGTGTGAAC 
CCGGGGCGCC TCACCAAAGG CCAGGTGGGG GGCACCTTCG GCCGACTCTA CCTCAGGAGG 
CAGACCACAG GCGGTGAGGG GCGGCAGAGT CCGTGTGCTG CCGCCCAGGT GGTCAGGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA2 ( XM_005227073.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227130.1 Bos taurus polymerase (DNA directed), alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34791
Clone ID Related Accession (Same CDS sequence)	XM_005227073.3 , XM_005227073.4
Accession Version	XM_005227073.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha subunit B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACATA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGTGGGTT TGAACTCAGC CATTTCCCAG 
CAATGCCAGA ACCAGTTCCT TTCTGGAGCC CTGGCACTTA ACTCCTCTCG GGTCTGCTGG 
TGGGGACAGT GGCCTCGTTC CTTTGGGGGA GGGGTAGGAG TAGAAAACAA ATCTGTTAGT 
ATAATGCTTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227130.1
CDS	386..2200
Translation	MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTHKDCFTLETLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGSHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKVGLNSAISQQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVSIMLK

Target ORF information:

RefSeq Version	XM_005227073.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227073.3

ORF Insert Sequence:

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACATA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGTGGGTT TGAACTCAGC CATTTCCCAG 
CAATGCCAGA ACCAGTTCCT TTCTGGAGCC CTGGCACTTA ACTCCTCTCG GGTCTGCTGG 
TGGGGACAGT GGCCTCGTTC CTTTGGGGGA GGGGTAGGAG TAGAAAACAA ATCTGTTAGT 
ATAATGCTTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA2 ( XM_005227073.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227130.1 Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34791
Clone ID Related Accession (Same CDS sequence)	XM_005227073.3 , XM_005227073.4
Accession Version	XM_005227073.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha subunit B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227073.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACATA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGTGGGTT TGAACTCAGC CATTTCCCAG 
CAATGCCAGA ACCAGTTCCT TTCTGGAGCC CTGGCACTTA ACTCCTCTCG GGTCTGCTGG 
TGGGGACAGT GGCCTCGTTC CTTTGGGGGA GGGGTAGGAG TAGAAAACAA ATCTGTTAGT 
ATAATGCTTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227130.1
CDS	465..2279
Translation	MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTHKDCFTLETLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGSHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKVGLNSAISQQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVSIMLK

Target ORF information:

RefSeq Version	XM_005227073.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 2, accessory subunit (POLA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227073.4

ORF Insert Sequence:

ATGGCTGTGT CCGCCCAGCT CTTGGTGGAG GAGTTGCAGA TCTTCGGCCT AGAATGCGAG 
GAGGCTGTGA TTGAGAAATT GGTAGAATTG TGCATTCTGT ATGGACAGAA TGAAGAGGGA 
ATGGCCAGCG AGCTTATAGC CTTCTGCACC AGCACACATA AAGACTGCTT TACCTTAGAG 
ACCCTGAATT CTTTTGAGCA CGAGTTTCTG AGCAAAAGAG TATCCAAAAC CCGACATGGC 
GCCTCCAAGG ACAAGGGGCT CAGGCACGCA GGAGCCAGAG ACATTGTCTC CATACAAGAG 
CTAATTGAAG TGGAGGAGGA AGAGGAGACC CTCTTGAACT CTTACACCAC ACCCTCTAAG 
GGTTCTCAGA AGCGAACTAT CACCACCCCA GAAACCCCCC TCACAAAGAG GAGTGTGTCT 
GCTCGGAGCC CCCATCAGCT CCTCTCACCA TCGAGTTTCT CTCCAAGCGC TACTCCCCCT 
CAGAAATACA GCTCGAGAAG TAACCGGGGA GAAGTGGTTA CCTCCTTTGG CTCGGCGCAG 
GGGGTGTCTT GGTCCGGGAG AGGAGGAGCT AGTCCCCTCA GCCTGAAGGT CTTGGGACAT 
CCAGAGCCGC TCACCGGGAG CTACAAATAC ATGTTTCAGA AGCTCCCAGA CATTCGAGAA 
GTTCTGACCT GTAAGATAGA AGAACTTGGC AGTGAACTCA AGGAACATTA CAAAATTGAG 
GCTTTTGCTC CTATTCTAGT CCCAGCACAG GAGCCCGTCA CCCTGCTGGG CCAGATCGGC 
TGTGACAGCA ACGGGAAGCT GAACCACAAG TCAGTGATTC TGGAGGGAGA TTTGGAACAT 
TCCTCAGGCG CTCAGATTCC AGTGGATCTA TCTGAGCTCA AAGAATATTC TCTATTTCCT 
GGACAGGTGG TGGTCATGGA AGGAATCAAC ACCACGGGTA GAAAACTTGT TGCCACCAGA 
CTCTACGAGG GTGTGCCGCT CCCATTCCAT CAGCCCGATG AAGAGGATGG AGATTCTGAG 
CAGTTCATGG TCCTGGTGGC CTGTGGGCCG TACACCACAT CTGACAGCAT CACCTTTGAC 
CCCCTGCTCG ACCTGATCAC CATCATCAAC CGCGACCGAC CGGACGTCTG CATCCTGTTT 
GGCCCTTTCC TGGACGCTAA GCATGAACAG GTTGAGAGCT GTCTGCTGAC GAGCTCATTT 
GAAGATGTTT TCAAGCAATG TCTCCGAACA ATTATTGAAG GAACACGAAG CTCCGGCTCC 
CACCTCATCA TCGTCCCGTC GCTGAGAGAC GTGCACCACG AGCCCGTGTA CCCACAACCT 
CCCTTCAGCT GCTCCGACCT GCTTCGAGAG GACAAAAAGC GAGTGAGGCT CGTGTCCGAG 
CCCTGCACCC TTTCCATCAA CGGAGTGATC TTTGGTTTGA CATCCACCGA CCTGCTGTTC 
CACATGGGGG CTGAGGAGAT CAGTAGTTCT TCTGGAACTT CAGACCGGTT CAGCCGAATT 
CTTAGACACA TCCTGACCCA GCGGAGCTAC TACCCACTCT ACCCACCCCA GGAGGACATG 
GCCATCGACT ACGAGAACTT CTACCTCTAC GCTCAGCTGC CCGTCACCCC CGACGTCTTC 
ATAGCCCCAT CAGAGCTGAG ATACTTCGTG AAGGTGGGTT TGAACTCAGC CATTTCCCAG 
CAATGCCAGA ACCAGTTCCT TTCTGGAGCC CTGGCACTTA ACTCCTCTCG GGTCTGCTGG 
TGGGGACAGT GGCCTCGTTC CTTTGGGGGA GGGGTAGGAG TAGAAAACAA ATCTGTTAGT 
ATAATGCTTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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POLA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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