BUB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	514777
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
General protein information	Preferred Names BUB1 mitotic checkpoint serine/threonine kinase Names mitotic checkpoint serine/threonine-protein kinase BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog budding uninhibited by benzimidazoles 1 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024998579.1	XP_024854347.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
NM_001102011.2	NP_001095481.1	mitotic checkpoint serine/threonine-protein kinase BUB1
XM_005212316.2	XP_005212373.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_005212316.3	XP_005212373.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_024998578.1	XP_024854346.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_010809723.2	XP_010808025.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BUB1	XP_001142040.1
Canis lupus familiaris (dog)	BUB1	XP_005630473.1
Bos taurus (cattle)	BUB1	NP_001095481.1
Gallus gallus (chicken)	BUB1	NP_001012888.1
Homo sapiens (human)	BUB1	NP_004327.1
Rattus norvegicus (Norway rat)	Bub1	NP_001099977.1
Danio rerio (zebrafish)	bub1	XP_002665685.2
Xenopus tropicalis (tropical clawed frog)	bub1	NP_001072830.1
	LOC696598	XP_002808098.1
Mus musculus (house mouse)	Bub1	NP_001106650.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220319	XM_024998579.1 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa37922	NM_001102011.2 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23694	XM_005212316.3 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49088	XM_024998578.1 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49088	XM_010809723.2 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa23694	XM_005212316.2	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( XM_024998579.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854347.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220319
Clone ID Related Accession (Same CDS sequence)	XM_024998579.1
Accession Version	XM_024998579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCA TTCCCTTGGC AGGAACCGAG CACACTCCCA CCTCCAAGCG CACAGTCCCA 
CCTCCAAGCA AGTTAATGTT TGAAGCCCAT ATGCAGAGCT ACAAGGGCGA TGACCCACTT 
GGTGAATGGG AAAGTTACAT GCAATGGGTA GAAGAGAATT TTCCTGAGAA TAAAGAATAC 
TTAACAACTT TATTAGAGCA TCTAATGAAG GAATTTTTAG ATAAGAAGAG ATATCACAAT 
GACCCAAGGT TCATCAATTA TTGTCTACAA TTTGCCAAGT GCAACAGTGA CCTTCACCAG 
TTTTTTGAGT TTCTGTACAA CCACGGTATC GGAACCCAAG CAGCTCCTCT GTACGTAGCC 
TGGGCCGGGC ATCTGGAAGC CCAGGGGGAG CGGCAGCACG CCAATGCCGT TTTTCGGAGA 
GGAATTCAGA ACCAGGCCGA ACCTAGAGAG CTGCTGCAAC AACAGTACAG GATGTTTCAG 
ACACGCCTCA CTGAAACCCA TTTGCCTACT CAAGTAAGAA CCTCAGAACC TCTGCATGAT 
GCTCAGATTT TAAATCAAAT GGTAACATCA AGCTCAAATC CAGGAAGTAA CCCAGCCTGC 
ATTTCTAAGA ATCAGGGTCC ACAACTTTCT GGAGCAACAC CTGCAGCTGG TGATAAAGGG 
TCGGACACGG AACAAAGGGT GATCACGATT TCCAAATCAG AATATTCTGC ACACCCGTCT 
GTGGCAGACA AAGCTGATGT TCAGCAGGTT GTCATGTATT GCAAGGAGAA GCTTATTCGC 
GGAGAATCGG AGTTTTCCTT TGAAGAGCTG AGAGCCCAGA AATACAATCT ACGGAGAAAG 
CATGAACAAT GGGTAAATGA AGAGAGGCAT TATATGAAAA GGAAAGAAGC AAATGCTTTG 
GAAGAACAAC TATTAAAACA GAAAATGGAT GAGCTTCATA AGAAGTTGCA CCAAGTGGTG 
GAGATGTCGC AGGAGAACCT GCCCTCTTCC CAGGGGACAT CTGAGGTCAG CCCAGCATGT 
TTGGAGCCGC ATACAGACTC CCAGCAGGAA CTAAGAGCTC CGGGTCTTCC AGCCATCTCT 
CAGCAGACCT CGAAGAATGT GGACGAGAAA CCCAGAGAGG CATCTTCTGT TCCTCGTATG 
GCGAATGCTG CCACCACTGC TTTGGTGTCC CCAGCCGCCA ACCAGGGTGT AGCTTGCCCG 
GCTCCTTTGG CAGGCCAGCA CGTGACAGAT GCCGTGTTTT CAGGGGGCAG CAGAGATGCC 
AGGTGTGTGA ACAAAAGTAC TCACGAATTC AGGCCACAGA GTGGAGCAGA GTTAAAAGAA 
GGGTGTGAAA CACAGAAGGT TGTTAACACG AGTTCCTTTC ACACAACTCC AAACACATCT 
CTGGGACTGG TTCAGGCAAC ACCATCCAAA GCGCAGCCAT CACCCACTGT GCACACGAAG 
GAGGCTCTCG GTTTCATCAT GAATATGTTT CAGGCTCCTA CACTTCCTGA TATTTCTGAT 
GACAAAGAGG AATGGCCATC TCTAAGTCAA AATGAGTTCG AAGCCCAGTT TCAAAAAAAT 
GCACCATCTT CTGGGGCTTG GGGAGTCAAT AAGATCATCT CTTCTTTGTC ATCTGCTTTT 
CCTGTATTTG AAGATGGAAA CAAAGAAAAT TATGGATTAC CACAGCTGAA AAATAAACCT 
GCAGGAGCCA GGACCTTTGG AGAACGTTCC GTGAGCAGGT TTCCTGCAAA ACCAAATGAA 
GTGCCTCACA CTGACGAGTT TCTGGATGAT TCAACCGTGT GGGGTGTCCG CTGCAACAAA 
AGCCTGGCGC CCAGCCCCAA GAGCCCAGGA GACTTTAGGT CCGCCGCGCA GCTGGCGTCT 
ACACCCTTCC ACAGGCTTCC CGCGGACTGC GTGCACCCAC CAGAAGATAA AGAAAATATG 
GTAGCAAACC AGCACACCCG TGTGGCTTTG GATTCTTGTG AAAGAAACAA GGTGGAACCC 
TTGAAAGAGA GAAAGTGCAG TCCAGTTGAA GAGACAAGCC CAGACCCAGT GTGCCCCGCA 
GATGGCCCTT CGGCATCCTT GCTCCATCTG GGCCAACCTG CTGTAGCCGT GCTGATGAGT 
GAGGCAGAGC AGGGTCTCAA GGCCTGTAAA GTCACAGACC CCGACTTTGC CACTGCAGAG 
GATCCGGCAG AAGCCAGTGC TGAGCTCCAG GCTGAACTGA TGCCGGCTGG CCCGCTTGGG 
AATGTCGACG CTCCAAACTT CACTGTTGAG AACCCTTGGG ATGAGGAGTT GATTCTCAAA 
CTTTTATCCG GGCTTTCTAA ACCAGTGAGT TCCTATCCAA ATACTTATGA ATGGCCATGT 
AAGCTTCCAG CCATCAAGCC CAAGACTGAA TTCCAATTAG GTTCTTTGCT CGTCTACGTT 
GATCACTTTA TTGGACAAGG GGCTTTCGCC CAAGTCTATG AAGTTACCCA TGGAGATGTG 
AACAACAGCA AAAATAAGGA GAAATTTGCT TTGAAGGTCC AGAAGCCTCC CAACCTCTGG 
GAATTCTACA TTGGGACCCA GCTGATGGAA AGACTAAAGC CAAGTATGCA ACACATGTTT 
ATCAAATTTT ATTCAGCCCA CCTGTTCCAG AATGGCAGCG TGTTAGTAGG AGACCTCTAC 
AGTTATGGCA CATTATTAAA TGCCATTAAT CTCTATAAAA ATACCCCTGA AAAAGTGATG 
CCTCAGGCAC TAGTCCTCGC TTTTGCTATC AGAATGCTCT GCATGATCCA GCAAGTCCAT 
GACTGTGAAA TCATCCACGG AGACATCAAG CCAGACAATT TCATACTCGG AAACAGATTT 
TTGGAACAAG ACAACAAAGA TGATGACTTA TCTAACGGCT TGGTCCTGAT TGACCTGGGT 
CAGAGTATAG ACATGAAACT TTTTCCCAAA GGAACTGCAT TTACAGGAAA GTGTGAAACT 
TCTGGTTTCC AGTGTATTGA GATGCTCAGT AACAAGCCAT GGAACTACCA GATTGATTAC 
TTTGGAGTTG CTGCAACCAT ATACTGCATG CTTTTTGGCA CCTACATGAA AGTGAAAAAT 
GAAGGGGGCA TCTGGAAGCC TGAAGGTCTT TTCAGAAGGC TTCCTCACTT GGACATGTGG 
AATGAATTTT TTCACATAAT GTTGAATATA CCCGATTGTC ATCATCTTCC ATCTTTGGAT 
TTGTTAAGGC AGAAGCTAAA GAAAACATTT CAAGAACACT ATACCAGCAA GATCAAGACC 
CTGTGTAACA GGCTAATTGT ACTGCTCTTA GAATATAAGC GTTCTCGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854347.1
CDS	370..3663
Translation	MFFIPLAGTEHTPTSKRTVPPPSKLMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGSDTEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_024998579.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998579.1

ORF Insert Sequence:

ATGTTTTTCA TTCCCTTGGC AGGAACCGAG CACACTCCCA CCTCCAAGCG CACAGTCCCA 
CCTCCAAGCA AGTTAATGTT TGAAGCCCAT ATGCAGAGCT ACAAGGGCGA TGACCCACTT 
GGTGAATGGG AAAGTTACAT GCAATGGGTA GAAGAGAATT TTCCTGAGAA TAAAGAATAC 
TTAACAACTT TATTAGAGCA TCTAATGAAG GAATTTTTAG ATAAGAAGAG ATATCACAAT 
GACCCAAGGT TCATCAATTA TTGTCTACAA TTTGCCAAGT GCAACAGTGA CCTTCACCAG 
TTTTTTGAGT TTCTGTACAA CCACGGTATC GGAACCCAAG CAGCTCCTCT GTACGTAGCC 
TGGGCCGGGC ATCTGGAAGC CCAGGGGGAG CGGCAGCACG CCAATGCCGT TTTTCGGAGA 
GGAATTCAGA ACCAGGCCGA ACCTAGAGAG CTGCTGCAAC AACAGTACAG GATGTTTCAG 
ACACGCCTCA CTGAAACCCA TTTGCCTACT CAAGTAAGAA CCTCAGAACC TCTGCATGAT 
GCTCAGATTT TAAATCAAAT GGTAACATCA AGCTCAAATC CAGGAAGTAA CCCAGCCTGC 
ATTTCTAAGA ATCAGGGTCC ACAACTTTCT GGAGCAACAC CTGCAGCTGG TGATAAAGGG 
TCGGACACGG AACAAAGGGT GATCACGATT TCCAAATCAG AATATTCTGC ACACCCGTCT 
GTGGCAGACA AAGCTGATGT TCAGCAGGTT GTCATGTATT GCAAGGAGAA GCTTATTCGC 
GGAGAATCGG AGTTTTCCTT TGAAGAGCTG AGAGCCCAGA AATACAATCT ACGGAGAAAG 
CATGAACAAT GGGTAAATGA AGAGAGGCAT TATATGAAAA GGAAAGAAGC AAATGCTTTG 
GAAGAACAAC TATTAAAACA GAAAATGGAT GAGCTTCATA AGAAGTTGCA CCAAGTGGTG 
GAGATGTCGC AGGAGAACCT GCCCTCTTCC CAGGGGACAT CTGAGGTCAG CCCAGCATGT 
TTGGAGCCGC ATACAGACTC CCAGCAGGAA CTAAGAGCTC CGGGTCTTCC AGCCATCTCT 
CAGCAGACCT CGAAGAATGT GGACGAGAAA CCCAGAGAGG CATCTTCTGT TCCTCGTATG 
GCGAATGCTG CCACCACTGC TTTGGTGTCC CCAGCCGCCA ACCAGGGTGT AGCTTGCCCG 
GCTCCTTTGG CAGGCCAGCA CGTGACAGAT GCCGTGTTTT CAGGGGGCAG CAGAGATGCC 
AGGTGTGTGA ACAAAAGTAC TCACGAATTC AGGCCACAGA GTGGAGCAGA GTTAAAAGAA 
GGGTGTGAAA CACAGAAGGT TGTTAACACG AGTTCCTTTC ACACAACTCC AAACACATCT 
CTGGGACTGG TTCAGGCAAC ACCATCCAAA GCGCAGCCAT CACCCACTGT GCACACGAAG 
GAGGCTCTCG GTTTCATCAT GAATATGTTT CAGGCTCCTA CACTTCCTGA TATTTCTGAT 
GACAAAGAGG AATGGCCATC TCTAAGTCAA AATGAGTTCG AAGCCCAGTT TCAAAAAAAT 
GCACCATCTT CTGGGGCTTG GGGAGTCAAT AAGATCATCT CTTCTTTGTC ATCTGCTTTT 
CCTGTATTTG AAGATGGAAA CAAAGAAAAT TATGGATTAC CACAGCTGAA AAATAAACCT 
GCAGGAGCCA GGACCTTTGG AGAACGTTCC GTGAGCAGGT TTCCTGCAAA ACCAAATGAA 
GTGCCTCACA CTGACGAGTT TCTGGATGAT TCAACCGTGT GGGGTGTCCG CTGCAACAAA 
AGCCTGGCGC CCAGCCCCAA GAGCCCAGGA GACTTTAGGT CCGCCGCGCA GCTGGCGTCT 
ACACCCTTCC ACAGGCTTCC CGCGGACTGC GTGCACCCAC CAGAAGATAA AGAAAATATG 
GTAGCAAACC AGCACACCCG TGTGGCTTTG GATTCTTGTG AAAGAAACAA GGTGGAACCC 
TTGAAAGAGA GAAAGTGCAG TCCAGTTGAA GAGACAAGCC CAGACCCAGT GTGCCCCGCA 
GATGGCCCTT CGGCATCCTT GCTCCATCTG GGCCAACCTG CTGTAGCCGT GCTGATGAGT 
GAGGCAGAGC AGGGTCTCAA GGCCTGTAAA GTCACAGACC CCGACTTTGC CACTGCAGAG 
GATCCGGCAG AAGCCAGTGC TGAGCTCCAG GCTGAACTGA TGCCGGCTGG CCCGCTTGGG 
AATGTCGACG CTCCAAACTT CACTGTTGAG AACCCTTGGG ATGAGGAGTT GATTCTCAAA 
CTTTTATCCG GGCTTTCTAA ACCAGTGAGT TCCTATCCAA ATACTTATGA ATGGCCATGT 
AAGCTTCCAG CCATCAAGCC CAAGACTGAA TTCCAATTAG GTTCTTTGCT CGTCTACGTT 
GATCACTTTA TTGGACAAGG GGCTTTCGCC CAAGTCTATG AAGTTACCCA TGGAGATGTG 
AACAACAGCA AAAATAAGGA GAAATTTGCT TTGAAGGTCC AGAAGCCTCC CAACCTCTGG 
GAATTCTACA TTGGGACCCA GCTGATGGAA AGACTAAAGC CAAGTATGCA ACACATGTTT 
ATCAAATTTT ATTCAGCCCA CCTGTTCCAG AATGGCAGCG TGTTAGTAGG AGACCTCTAC 
AGTTATGGCA CATTATTAAA TGCCATTAAT CTCTATAAAA ATACCCCTGA AAAAGTGATG 
CCTCAGGCAC TAGTCCTCGC TTTTGCTATC AGAATGCTCT GCATGATCCA GCAAGTCCAT 
GACTGTGAAA TCATCCACGG AGACATCAAG CCAGACAATT TCATACTCGG AAACAGATTT 
TTGGAACAAG ACAACAAAGA TGATGACTTA TCTAACGGCT TGGTCCTGAT TGACCTGGGT 
CAGAGTATAG ACATGAAACT TTTTCCCAAA GGAACTGCAT TTACAGGAAA GTGTGAAACT 
TCTGGTTTCC AGTGTATTGA GATGCTCAGT AACAAGCCAT GGAACTACCA GATTGATTAC 
TTTGGAGTTG CTGCAACCAT ATACTGCATG CTTTTTGGCA CCTACATGAA AGTGAAAAAT 
GAAGGGGGCA TCTGGAAGCC TGAAGGTCTT TTCAGAAGGC TTCCTCACTT GGACATGTGG 
AATGAATTTT TTCACATAAT GTTGAATATA CCCGATTGTC ATCATCTTCC ATCTTTGGAT 
TTGTTAAGGC AGAAGCTAAA GAAAACATTT CAAGAACACT ATACCAGCAA GATCAAGACC 
CTGTGTAACA GGCTAATTGT ACTGCTCTTA GAATATAAGC GTTCTCGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( NM_001102011.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095481.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Price & Availability↑

CloneID	OBa37922
Clone ID Related Accession (Same CDS sequence)	NM_001102011.2
Accession Version	NM_001102011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC148104.1. On Aug 30, 2012 this sequence version replaced NM_001102011.1. ##Evidence-Data-START## Transcript exon combination :: BC148104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145433 [ECO:0000348] ##Evidence-Data-END##

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACG ATTTCCAAAT CAGAATATTC TGCACACCCG 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTTGAAGAG CTGAGAGCCC AGAAATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGTT GCACCAAGTG 
GTGGAGATGT CGCAGGAGAA CCTGCCCTCT TCCCAGGGGA CATCTGAGGT CAGCCCAGCA 
TGTTTGGAGC CGCATACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCATC 
TCTCAGCAGA CCTCGAAGAA TGTGGACGAG AAACCCAGAG AGGCATCTTC TGTTCCTCGT 
ATGGCGAATG CTGCCACCAC TGCTTTGGTG TCCCCAGCCG CCAACCAGGG TGTAGCTTGC 
CCGGCTCCTT TGGCAGGCCA GCACGTGACA GATGCCGTGT TTTCAGGGGG CAGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCACGAA TTCAGGCCAC AGAGTGGAGC AGAGTTAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACGAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGCGCAGC CATCACCCAC TGTGCACACG 
AAGGAGGCTC TCGGTTTCAT CATGAATATG TTTCAGGCTC CTACACTTCC TGATATTTCT 
GATGACAAAG AGGAATGGCC ATCTCTAAGT CAAAATGAGT TCGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCT GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ACACTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CGCCCAGCCC CAAGAGCCCA GGAGACTTTA GGTCCGCCGC GCAGCTGGCG 
TCTACACCCT TCCACAGGCT TCCCGCGGAC TGCGTGCACC CACCAGAAGA TAAAGAAAAT 
ATGGTAGCAA ACCAGCACAC CCGTGTGGCT TTGGATTCTT GTGAAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAAGTG CAGTCCAGTT GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
GCAGATGGCC CTTCGGCATC CTTGCTCCAT CTGGGCCAAC CTGCTGTAGC CGTGCTGATG 
AGTGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT TGCCACTGCA 
GAGGATCCGG CAGAAGCCAG TGCTGAGCTC CAGGCTGAAC TGATGCCGGC TGGCCCGCTT 
GGGAATGTCG ACGCTCCAAA CTTCACTGTT GAGAACCCTT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGCCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAAT TAGGTTCTTT GCTCGTCTAC 
GTTGATCACT TTATTGGACA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAATTCT ACATTGGGAC CCAGCTGATG GAAAGACTAA AGCCAAGTAT GCAACACATG 
TTTATCAAAT TTTATTCAGC CCACCTGTTC CAGAATGGCA GCGTGTTAGT AGGAGACCTC 
TACAGTTATG GCACATTATT AAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCT ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTAACG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCC AAAGGAACTG CATTTACAGG AAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTTTG GCACCTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTCAGAA GGCTTCCTCA CTTGGACATG 
TGGAATGAAT TTTTTCACAT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTATACCAG CAAGATCAAG 
ACCCTGTGTA ACAGGCTAAT TGTACTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095481.1
CDS	74..3310
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	NM_001102011.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102011.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACG ATTTCCAAAT CAGAATATTC TGCACACCCG 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTTGAAGAG CTGAGAGCCC AGAAATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGTT GCACCAAGTG 
GTGGAGATGT CGCAGGAGAA CCTGCCCTCT TCCCAGGGGA CATCTGAGGT CAGCCCAGCA 
TGTTTGGAGC CGCATACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCATC 
TCTCAGCAGA CCTCGAAGAA TGTGGACGAG AAACCCAGAG AGGCATCTTC TGTTCCTCGT 
ATGGCGAATG CTGCCACCAC TGCTTTGGTG TCCCCAGCCG CCAACCAGGG TGTAGCTTGC 
CCGGCTCCTT TGGCAGGCCA GCACGTGACA GATGCCGTGT TTTCAGGGGG CAGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCACGAA TTCAGGCCAC AGAGTGGAGC AGAGTTAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACGAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGCGCAGC CATCACCCAC TGTGCACACG 
AAGGAGGCTC TCGGTTTCAT CATGAATATG TTTCAGGCTC CTACACTTCC TGATATTTCT 
GATGACAAAG AGGAATGGCC ATCTCTAAGT CAAAATGAGT TCGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCT GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ACACTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CGCCCAGCCC CAAGAGCCCA GGAGACTTTA GGTCCGCCGC GCAGCTGGCG 
TCTACACCCT TCCACAGGCT TCCCGCGGAC TGCGTGCACC CACCAGAAGA TAAAGAAAAT 
ATGGTAGCAA ACCAGCACAC CCGTGTGGCT TTGGATTCTT GTGAAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAAGTG CAGTCCAGTT GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
GCAGATGGCC CTTCGGCATC CTTGCTCCAT CTGGGCCAAC CTGCTGTAGC CGTGCTGATG 
AGTGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT TGCCACTGCA 
GAGGATCCGG CAGAAGCCAG TGCTGAGCTC CAGGCTGAAC TGATGCCGGC TGGCCCGCTT 
GGGAATGTCG ACGCTCCAAA CTTCACTGTT GAGAACCCTT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGCCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAAT TAGGTTCTTT GCTCGTCTAC 
GTTGATCACT TTATTGGACA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAATTCT ACATTGGGAC CCAGCTGATG GAAAGACTAA AGCCAAGTAT GCAACACATG 
TTTATCAAAT TTTATTCAGC CCACCTGTTC CAGAATGGCA GCGTGTTAGT AGGAGACCTC 
TACAGTTATG GCACATTATT AAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCT ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTAACG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCC AAAGGAACTG CATTTACAGG AAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTTTG GCACCTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTCAGAA GGCTTCCTCA CTTGGACATG 
TGGAATGAAT TTTTTCACAT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTATACCAG CAAGATCAAG 
ACCCTGTGTA ACAGGCTAAT TGTACTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_005212316.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212373.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23694
Clone ID Related Accession (Same CDS sequence)	XM_005212316.2 , XM_005212316.3
Accession Version	XM_005212316.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCGGACAC GGAACAAAGG GTGATCACGA TTTCCAAATC AGAATATTCT 
GCACACCCGT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTTGAAGAGC TGAGAGCCCA GAAATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGTTG 
CACCAAGTGG TGGAGATGTC GCAGGAGAAC CTGCCCTCTT CCCAGGGGAC ATCTGAGGTC 
AGCCCAGCAT GTTTGGAGCC GCATACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCATCT CTCAGCAGAC CTCGAAGAAT GTGGACGAGA AACCCAGAGA GGCATCTTCT 
GTTCCTCGTA TGGCGAATGC TGCCACCACT GCTTTGGTGT CCCCAGCCGC CAACCAGGGT 
GTAGCTTGCC CGGCTCCTTT GGCAGGCCAG CACGTGACAG ATGCCGTGTT TTCAGGGGGC 
AGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCACGAAT TCAGGCCACA GAGTGGAGCA 
GAGTTAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CGAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGCGCAGCC ATCACCCACT 
GTGCACACGA AGGAGGCTCT CGGTTTCATC ATGAATATGT TTCAGGCTCC TACACTTCCT 
GATATTTCTG ATGACAAAGA GGAATGGCCA TCTCTAAGTC AAAATGAGTT CGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCTG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA CACTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC GCCCAGCCCC AAGAGCCCAG GAGACTTTAG GTCCGCCGCG 
CAGCTGGCGT CTACACCCTT CCACAGGCTT CCCGCGGACT GCGTGCACCC ACCAGAAGAT 
AAAGAAAATA TGGTAGCAAA CCAGCACACC CGTGTGGCTT TGGATTCTTG TGAAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAAGTGC AGTCCAGTTG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCG CAGATGGCCC TTCGGCATCC TTGCTCCATC TGGGCCAACC TGCTGTAGCC 
GTGCTGATGA GTGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTT 
GCCACTGCAG AGGATCCGGC AGAAGCCAGT GCTGAGCTCC AGGCTGAACT GATGCCGGCT 
GGCCCGCTTG GGAATGTCGA CGCTCCAAAC TTCACTGTTG AGAACCCTTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGCCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAATT AGGTTCTTTG 
CTCGTCTACG TTGATCACTT TATTGGACAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAATTCTA CATTGGGACC CAGCTGATGG AAAGACTAAA GCCAAGTATG 
CAACACATGT TTATCAAATT TTATTCAGCC CACCTGTTCC AGAATGGCAG CGTGTTAGTA 
GGAGACCTCT ACAGTTATGG CACATTATTA AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCTA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTAACGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCCA AAGGAACTGC ATTTACAGGA 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTTTGG CACCTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTCAGAAG GCTTCCTCAC 
TTGGACATGT GGAATGAATT TTTTCACATA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTATACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTACTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212373.1
CDS	101..3346
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGSDTEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_005212316.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212316.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCGGACAC GGAACAAAGG GTGATCACGA TTTCCAAATC AGAATATTCT 
GCACACCCGT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTTGAAGAGC TGAGAGCCCA GAAATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGTTG 
CACCAAGTGG TGGAGATGTC GCAGGAGAAC CTGCCCTCTT CCCAGGGGAC ATCTGAGGTC 
AGCCCAGCAT GTTTGGAGCC GCATACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCATCT CTCAGCAGAC CTCGAAGAAT GTGGACGAGA AACCCAGAGA GGCATCTTCT 
GTTCCTCGTA TGGCGAATGC TGCCACCACT GCTTTGGTGT CCCCAGCCGC CAACCAGGGT 
GTAGCTTGCC CGGCTCCTTT GGCAGGCCAG CACGTGACAG ATGCCGTGTT TTCAGGGGGC 
AGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCACGAAT TCAGGCCACA GAGTGGAGCA 
GAGTTAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CGAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGCGCAGCC ATCACCCACT 
GTGCACACGA AGGAGGCTCT CGGTTTCATC ATGAATATGT TTCAGGCTCC TACACTTCCT 
GATATTTCTG ATGACAAAGA GGAATGGCCA TCTCTAAGTC AAAATGAGTT CGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCTG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA CACTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC GCCCAGCCCC AAGAGCCCAG GAGACTTTAG GTCCGCCGCG 
CAGCTGGCGT CTACACCCTT CCACAGGCTT CCCGCGGACT GCGTGCACCC ACCAGAAGAT 
AAAGAAAATA TGGTAGCAAA CCAGCACACC CGTGTGGCTT TGGATTCTTG TGAAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAAGTGC AGTCCAGTTG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCG CAGATGGCCC TTCGGCATCC TTGCTCCATC TGGGCCAACC TGCTGTAGCC 
GTGCTGATGA GTGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTT 
GCCACTGCAG AGGATCCGGC AGAAGCCAGT GCTGAGCTCC AGGCTGAACT GATGCCGGCT 
GGCCCGCTTG GGAATGTCGA CGCTCCAAAC TTCACTGTTG AGAACCCTTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGCCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAATT AGGTTCTTTG 
CTCGTCTACG TTGATCACTT TATTGGACAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAATTCTA CATTGGGACC CAGCTGATGG AAAGACTAAA GCCAAGTATG 
CAACACATGT TTATCAAATT TTATTCAGCC CACCTGTTCC AGAATGGCAG CGTGTTAGTA 
GGAGACCTCT ACAGTTATGG CACATTATTA AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCTA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTAACGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCCA AAGGAACTGC ATTTACAGGA 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTTTGG CACCTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTCAGAAG GCTTCCTCAC 
TTGGACATGT GGAATGAATT TTTTCACATA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTATACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTACTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_005212316.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212373.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23694
Clone ID Related Accession (Same CDS sequence)	XM_005212316.2 , XM_005212316.3
Accession Version	XM_005212316.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCGGACAC GGAACAAAGG GTGATCACGA TTTCCAAATC AGAATATTCT 
GCACACCCGT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTTGAAGAGC TGAGAGCCCA GAAATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGTTG 
CACCAAGTGG TGGAGATGTC GCAGGAGAAC CTGCCCTCTT CCCAGGGGAC ATCTGAGGTC 
AGCCCAGCAT GTTTGGAGCC GCATACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCATCT CTCAGCAGAC CTCGAAGAAT GTGGACGAGA AACCCAGAGA GGCATCTTCT 
GTTCCTCGTA TGGCGAATGC TGCCACCACT GCTTTGGTGT CCCCAGCCGC CAACCAGGGT 
GTAGCTTGCC CGGCTCCTTT GGCAGGCCAG CACGTGACAG ATGCCGTGTT TTCAGGGGGC 
AGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCACGAAT TCAGGCCACA GAGTGGAGCA 
GAGTTAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CGAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGCGCAGCC ATCACCCACT 
GTGCACACGA AGGAGGCTCT CGGTTTCATC ATGAATATGT TTCAGGCTCC TACACTTCCT 
GATATTTCTG ATGACAAAGA GGAATGGCCA TCTCTAAGTC AAAATGAGTT CGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCTG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA CACTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC GCCCAGCCCC AAGAGCCCAG GAGACTTTAG GTCCGCCGCG 
CAGCTGGCGT CTACACCCTT CCACAGGCTT CCCGCGGACT GCGTGCACCC ACCAGAAGAT 
AAAGAAAATA TGGTAGCAAA CCAGCACACC CGTGTGGCTT TGGATTCTTG TGAAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAAGTGC AGTCCAGTTG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCG CAGATGGCCC TTCGGCATCC TTGCTCCATC TGGGCCAACC TGCTGTAGCC 
GTGCTGATGA GTGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTT 
GCCACTGCAG AGGATCCGGC AGAAGCCAGT GCTGAGCTCC AGGCTGAACT GATGCCGGCT 
GGCCCGCTTG GGAATGTCGA CGCTCCAAAC TTCACTGTTG AGAACCCTTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGCCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAATT AGGTTCTTTG 
CTCGTCTACG TTGATCACTT TATTGGACAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAATTCTA CATTGGGACC CAGCTGATGG AAAGACTAAA GCCAAGTATG 
CAACACATGT TTATCAAATT TTATTCAGCC CACCTGTTCC AGAATGGCAG CGTGTTAGTA 
GGAGACCTCT ACAGTTATGG CACATTATTA AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCTA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTAACGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCCA AAGGAACTGC ATTTACAGGA 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTTTGG CACCTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTCAGAAG GCTTCCTCAC 
TTGGACATGT GGAATGAATT TTTTCACATA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTATACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTACTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212373.1
CDS	127..3372
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGSDTEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_005212316.3
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212316.3

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT TTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGTAC AACCACGGTA TCGGAACCCA AGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCATCTGGAA GCCCAGGGGG AGCGGCAGCA CGCCAATGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGCCTA CTCAAGTAAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAGCTCAAA TCCAGGAAGT 
AACCCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTT CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCGGACAC GGAACAAAGG GTGATCACGA TTTCCAAATC AGAATATTCT 
GCACACCCGT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTTGAAGAGC TGAGAGCCCA GAAATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGTTG 
CACCAAGTGG TGGAGATGTC GCAGGAGAAC CTGCCCTCTT CCCAGGGGAC ATCTGAGGTC 
AGCCCAGCAT GTTTGGAGCC GCATACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCATCT CTCAGCAGAC CTCGAAGAAT GTGGACGAGA AACCCAGAGA GGCATCTTCT 
GTTCCTCGTA TGGCGAATGC TGCCACCACT GCTTTGGTGT CCCCAGCCGC CAACCAGGGT 
GTAGCTTGCC CGGCTCCTTT GGCAGGCCAG CACGTGACAG ATGCCGTGTT TTCAGGGGGC 
AGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCACGAAT TCAGGCCACA GAGTGGAGCA 
GAGTTAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CGAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGCGCAGCC ATCACCCACT 
GTGCACACGA AGGAGGCTCT CGGTTTCATC ATGAATATGT TTCAGGCTCC TACACTTCCT 
GATATTTCTG ATGACAAAGA GGAATGGCCA TCTCTAAGTC AAAATGAGTT CGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCTG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA CACTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC GCCCAGCCCC AAGAGCCCAG GAGACTTTAG GTCCGCCGCG 
CAGCTGGCGT CTACACCCTT CCACAGGCTT CCCGCGGACT GCGTGCACCC ACCAGAAGAT 
AAAGAAAATA TGGTAGCAAA CCAGCACACC CGTGTGGCTT TGGATTCTTG TGAAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAAGTGC AGTCCAGTTG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCG CAGATGGCCC TTCGGCATCC TTGCTCCATC TGGGCCAACC TGCTGTAGCC 
GTGCTGATGA GTGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTT 
GCCACTGCAG AGGATCCGGC AGAAGCCAGT GCTGAGCTCC AGGCTGAACT GATGCCGGCT 
GGCCCGCTTG GGAATGTCGA CGCTCCAAAC TTCACTGTTG AGAACCCTTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGCCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAATT AGGTTCTTTG 
CTCGTCTACG TTGATCACTT TATTGGACAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAATTCTA CATTGGGACC CAGCTGATGG AAAGACTAAA GCCAAGTATG 
CAACACATGT TTATCAAATT TTATTCAGCC CACCTGTTCC AGAATGGCAG CGTGTTAGTA 
GGAGACCTCT ACAGTTATGG CACATTATTA AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCTA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTAACGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCCA AAGGAACTGC ATTTACAGGA 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTTTGG CACCTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTCAGAAG GCTTCCTCAC 
TTGGACATGT GGAATGAATT TTTTCACATA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTATACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTACTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_024998578.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854346.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

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CloneID	OBa49088
Clone ID Related Accession (Same CDS sequence)	XM_024998578.1 , XM_010809723.2
Accession Version	XM_024998578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAG CCCATATGCA GAGCTACAAG GGCGATGACC CACTTGGTGA ATGGGAAAGT 
TACATGCAAT GGGTAGAAGA GAATTTTCCT GAGAATAAAG AATACTTAAC AACTTTATTA 
GAGCATCTAA TGAAGGAATT TTTAGATAAG AAGAGATATC ACAATGACCC AAGGTTCATC 
AATTATTGTC TACAATTTGC CAAGTGCAAC AGTGACCTTC ACCAGTTTTT TGAGTTTCTG 
TACAACCACG GTATCGGAAC CCAAGCAGCT CCTCTGTACG TAGCCTGGGC CGGGCATCTG 
GAAGCCCAGG GGGAGCGGCA GCACGCCAAT GCCGTTTTTC GGAGAGGAAT TCAGAACCAG 
GCCGAACCTA GAGAGCTGCT GCAACAACAG TACAGGATGT TTCAGACACG CCTCACTGAA 
ACCCATTTGC CTACTCAAGT AAGAACCTCA GAACCTCTGC ATGATGCTCA GATTTTAAAT 
CAAATGGTAA CATCAAGCTC AAATCCAGGA AGTAACCCAG CCTGCATTTC TAAGAATCAG 
GGTCCACAAC TTTCTGGAGC AACACCTGCA GCTGGTGATA AAGGGTCGGA CACGGAACAA 
AGGGTGATCA CGATTTCCAA ATCAGAATAT TCTGCACACC CGTCTGTGGC AGACAAAGCT 
GATGTTCAGC AGGTTGTCAT GTATTGCAAG GAGAAGCTTA TTCGCGGAGA ATCGGAGTTT 
TCCTTTGAAG AGCTGAGAGC CCAGAAATAC AATCTACGGA GAAAGCATGA ACAATGGGTA 
AATGAAGAGA GGCATTATAT GAAAAGGAAA GAAGCAAATG CTTTGGAAGA ACAACTATTA 
AAACAGAAAA TGGATGAGCT TCATAAGAAG TTGCACCAAG TGGTGGAGAT GTCGCAGGAG 
AACCTGCCCT CTTCCCAGGG GACATCTGAG GTCAGCCCAG CATGTTTGGA GCCGCATACA 
GACTCCCAGC AGGAACTAAG AGCTCCGGGT CTTCCAGCCA TCTCTCAGCA GACCTCGAAG 
AATGTGGACG AGAAACCCAG AGAGGCATCT TCTGTTCCTC GTATGGCGAA TGCTGCCACC 
ACTGCTTTGG TGTCCCCAGC CGCCAACCAG GGTGTAGCTT GCCCGGCTCC TTTGGCAGGC 
CAGCACGTGA CAGATGCCGT GTTTTCAGGG GGCAGCAGAG ATGCCAGGTG TGTGAACAAA 
AGTACTCACG AATTCAGGCC ACAGAGTGGA GCAGAGTTAA AAGAAGGGTG TGAAACACAG 
AAGGTTGTTA ACACGAGTTC CTTTCACACA ACTCCAAACA CATCTCTGGG ACTGGTTCAG 
GCAACACCAT CCAAAGCGCA GCCATCACCC ACTGTGCACA CGAAGGAGGC TCTCGGTTTC 
ATCATGAATA TGTTTCAGGC TCCTACACTT CCTGATATTT CTGATGACAA AGAGGAATGG 
CCATCTCTAA GTCAAAATGA GTTCGAAGCC CAGTTTCAAA AAAATGCACC ATCTTCTGGG 
GCTTGGGGAG TCAATAAGAT CATCTCTTCT TTGTCATCTG CTTTTCCTGT ATTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CTGAAAAATA AACCTGCAGG AGCCAGGACC 
TTTGGAGAAC GTTCCGTGAG CAGGTTTCCT GCAAAACCAA ATGAAGTGCC TCACACTGAC 
GAGTTTCTGG ATGATTCAAC CGTGTGGGGT GTCCGCTGCA ACAAAAGCCT GGCGCCCAGC 
CCCAAGAGCC CAGGAGACTT TAGGTCCGCC GCGCAGCTGG CGTCTACACC CTTCCACAGG 
CTTCCCGCGG ACTGCGTGCA CCCACCAGAA GATAAAGAAA ATATGGTAGC AAACCAGCAC 
ACCCGTGTGG CTTTGGATTC TTGTGAAAGA AACAAGGTGG AACCCTTGAA AGAGAGAAAG 
TGCAGTCCAG TTGAAGAGAC AAGCCCAGAC CCAGTGTGCC CCGCAGATGG CCCTTCGGCA 
TCCTTGCTCC ATCTGGGCCA ACCTGCTGTA GCCGTGCTGA TGAGTGAGGC AGAGCAGGGT 
CTCAAGGCCT GTAAAGTCAC AGACCCCGAC TTTGCCACTG CAGAGGATCC GGCAGAAGCC 
AGTGCTGAGC TCCAGGCTGA ACTGATGCCG GCTGGCCCGC TTGGGAATGT CGACGCTCCA 
AACTTCACTG TTGAGAACCC TTGGGATGAG GAGTTGATTC TCAAACTTTT ATCCGGGCTT 
TCTAAACCAG TGAGTTCCTA TCCAAATACT TATGAATGGC CATGTAAGCT TCCAGCCATC 
AAGCCCAAGA CTGAATTCCA ATTAGGTTCT TTGCTCGTCT ACGTTGATCA CTTTATTGGA 
CAAGGGGCTT TCGCCCAAGT CTATGAAGTT ACCCATGGAG ATGTGAACAA CAGCAAAAAT 
AAGGAGAAAT TTGCTTTGAA GGTCCAGAAG CCTCCCAACC TCTGGGAATT CTACATTGGG 
ACCCAGCTGA TGGAAAGACT AAAGCCAAGT ATGCAACACA TGTTTATCAA ATTTTATTCA 
GCCCACCTGT TCCAGAATGG CAGCGTGTTA GTAGGAGACC TCTACAGTTA TGGCACATTA 
TTAAATGCCA TTAATCTCTA TAAAAATACC CCTGAAAAAG TGATGCCTCA GGCACTAGTC 
CTCGCTTTTG CTATCAGAAT GCTCTGCATG ATCCAGCAAG TCCATGACTG TGAAATCATC 
CACGGAGACA TCAAGCCAGA CAATTTCATA CTCGGAAACA GATTTTTGGA ACAAGACAAC 
AAAGATGATG ACTTATCTAA CGGCTTGGTC CTGATTGACC TGGGTCAGAG TATAGACATG 
AAACTTTTTC CCAAAGGAAC TGCATTTACA GGAAAGTGTG AAACTTCTGG TTTCCAGTGT 
ATTGAGATGC TCAGTAACAA GCCATGGAAC TACCAGATTG ATTACTTTGG AGTTGCTGCA 
ACCATATACT GCATGCTTTT TGGCACCTAC ATGAAAGTGA AAAATGAAGG GGGCATCTGG 
AAGCCTGAAG GTCTTTTCAG AAGGCTTCCT CACTTGGACA TGTGGAATGA ATTTTTTCAC 
ATAATGTTGA ATATACCCGA TTGTCATCAT CTTCCATCTT TGGATTTGTT AAGGCAGAAG 
CTAAAGAAAA CATTTCAAGA ACACTATACC AGCAAGATCA AGACCCTGTG TAACAGGCTA 
ATTGTACTGC TCTTAGAATA TAAGCGTTCT CGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854346.1
CDS	246..3464
Translation	MFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGSDTEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_024998578.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998578.1

ORF Insert Sequence:

ATGTTTGAAG CCCATATGCA GAGCTACAAG GGCGATGACC CACTTGGTGA ATGGGAAAGT 
TACATGCAAT GGGTAGAAGA GAATTTTCCT GAGAATAAAG AATACTTAAC AACTTTATTA 
GAGCATCTAA TGAAGGAATT TTTAGATAAG AAGAGATATC ACAATGACCC AAGGTTCATC 
AATTATTGTC TACAATTTGC CAAGTGCAAC AGTGACCTTC ACCAGTTTTT TGAGTTTCTG 
TACAACCACG GTATCGGAAC CCAAGCAGCT CCTCTGTACG TAGCCTGGGC CGGGCATCTG 
GAAGCCCAGG GGGAGCGGCA GCACGCCAAT GCCGTTTTTC GGAGAGGAAT TCAGAACCAG 
GCCGAACCTA GAGAGCTGCT GCAACAACAG TACAGGATGT TTCAGACACG CCTCACTGAA 
ACCCATTTGC CTACTCAAGT AAGAACCTCA GAACCTCTGC ATGATGCTCA GATTTTAAAT 
CAAATGGTAA CATCAAGCTC AAATCCAGGA AGTAACCCAG CCTGCATTTC TAAGAATCAG 
GGTCCACAAC TTTCTGGAGC AACACCTGCA GCTGGTGATA AAGGGTCGGA CACGGAACAA 
AGGGTGATCA CGATTTCCAA ATCAGAATAT TCTGCACACC CGTCTGTGGC AGACAAAGCT 
GATGTTCAGC AGGTTGTCAT GTATTGCAAG GAGAAGCTTA TTCGCGGAGA ATCGGAGTTT 
TCCTTTGAAG AGCTGAGAGC CCAGAAATAC AATCTACGGA GAAAGCATGA ACAATGGGTA 
AATGAAGAGA GGCATTATAT GAAAAGGAAA GAAGCAAATG CTTTGGAAGA ACAACTATTA 
AAACAGAAAA TGGATGAGCT TCATAAGAAG TTGCACCAAG TGGTGGAGAT GTCGCAGGAG 
AACCTGCCCT CTTCCCAGGG GACATCTGAG GTCAGCCCAG CATGTTTGGA GCCGCATACA 
GACTCCCAGC AGGAACTAAG AGCTCCGGGT CTTCCAGCCA TCTCTCAGCA GACCTCGAAG 
AATGTGGACG AGAAACCCAG AGAGGCATCT TCTGTTCCTC GTATGGCGAA TGCTGCCACC 
ACTGCTTTGG TGTCCCCAGC CGCCAACCAG GGTGTAGCTT GCCCGGCTCC TTTGGCAGGC 
CAGCACGTGA CAGATGCCGT GTTTTCAGGG GGCAGCAGAG ATGCCAGGTG TGTGAACAAA 
AGTACTCACG AATTCAGGCC ACAGAGTGGA GCAGAGTTAA AAGAAGGGTG TGAAACACAG 
AAGGTTGTTA ACACGAGTTC CTTTCACACA ACTCCAAACA CATCTCTGGG ACTGGTTCAG 
GCAACACCAT CCAAAGCGCA GCCATCACCC ACTGTGCACA CGAAGGAGGC TCTCGGTTTC 
ATCATGAATA TGTTTCAGGC TCCTACACTT CCTGATATTT CTGATGACAA AGAGGAATGG 
CCATCTCTAA GTCAAAATGA GTTCGAAGCC CAGTTTCAAA AAAATGCACC ATCTTCTGGG 
GCTTGGGGAG TCAATAAGAT CATCTCTTCT TTGTCATCTG CTTTTCCTGT ATTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CTGAAAAATA AACCTGCAGG AGCCAGGACC 
TTTGGAGAAC GTTCCGTGAG CAGGTTTCCT GCAAAACCAA ATGAAGTGCC TCACACTGAC 
GAGTTTCTGG ATGATTCAAC CGTGTGGGGT GTCCGCTGCA ACAAAAGCCT GGCGCCCAGC 
CCCAAGAGCC CAGGAGACTT TAGGTCCGCC GCGCAGCTGG CGTCTACACC CTTCCACAGG 
CTTCCCGCGG ACTGCGTGCA CCCACCAGAA GATAAAGAAA ATATGGTAGC AAACCAGCAC 
ACCCGTGTGG CTTTGGATTC TTGTGAAAGA AACAAGGTGG AACCCTTGAA AGAGAGAAAG 
TGCAGTCCAG TTGAAGAGAC AAGCCCAGAC CCAGTGTGCC CCGCAGATGG CCCTTCGGCA 
TCCTTGCTCC ATCTGGGCCA ACCTGCTGTA GCCGTGCTGA TGAGTGAGGC AGAGCAGGGT 
CTCAAGGCCT GTAAAGTCAC AGACCCCGAC TTTGCCACTG CAGAGGATCC GGCAGAAGCC 
AGTGCTGAGC TCCAGGCTGA ACTGATGCCG GCTGGCCCGC TTGGGAATGT CGACGCTCCA 
AACTTCACTG TTGAGAACCC TTGGGATGAG GAGTTGATTC TCAAACTTTT ATCCGGGCTT 
TCTAAACCAG TGAGTTCCTA TCCAAATACT TATGAATGGC CATGTAAGCT TCCAGCCATC 
AAGCCCAAGA CTGAATTCCA ATTAGGTTCT TTGCTCGTCT ACGTTGATCA CTTTATTGGA 
CAAGGGGCTT TCGCCCAAGT CTATGAAGTT ACCCATGGAG ATGTGAACAA CAGCAAAAAT 
AAGGAGAAAT TTGCTTTGAA GGTCCAGAAG CCTCCCAACC TCTGGGAATT CTACATTGGG 
ACCCAGCTGA TGGAAAGACT AAAGCCAAGT ATGCAACACA TGTTTATCAA ATTTTATTCA 
GCCCACCTGT TCCAGAATGG CAGCGTGTTA GTAGGAGACC TCTACAGTTA TGGCACATTA 
TTAAATGCCA TTAATCTCTA TAAAAATACC CCTGAAAAAG TGATGCCTCA GGCACTAGTC 
CTCGCTTTTG CTATCAGAAT GCTCTGCATG ATCCAGCAAG TCCATGACTG TGAAATCATC 
CACGGAGACA TCAAGCCAGA CAATTTCATA CTCGGAAACA GATTTTTGGA ACAAGACAAC 
AAAGATGATG ACTTATCTAA CGGCTTGGTC CTGATTGACC TGGGTCAGAG TATAGACATG 
AAACTTTTTC CCAAAGGAAC TGCATTTACA GGAAAGTGTG AAACTTCTGG TTTCCAGTGT 
ATTGAGATGC TCAGTAACAA GCCATGGAAC TACCAGATTG ATTACTTTGG AGTTGCTGCA 
ACCATATACT GCATGCTTTT TGGCACCTAC ATGAAAGTGA AAAATGAAGG GGGCATCTGG 
AAGCCTGAAG GTCTTTTCAG AAGGCTTCCT CACTTGGACA TGTGGAATGA ATTTTTTCAC 
ATAATGTTGA ATATACCCGA TTGTCATCAT CTTCCATCTT TGGATTTGTT AAGGCAGAAG 
CTAAAGAAAA CATTTCAAGA ACACTATACC AGCAAGATCA AGACCCTGTG TAACAGGCTA 
ATTGTACTGC TCTTAGAATA TAAGCGTTCT CGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_010809723.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808025.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

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CloneID	OBa49088
Clone ID Related Accession (Same CDS sequence)	XM_024998578.1 , XM_010809723.2
Accession Version	XM_010809723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAG CCCATATGCA GAGCTACAAG GGCGATGACC CACTTGGTGA ATGGGAAAGT 
TACATGCAAT GGGTAGAAGA GAATTTTCCT GAGAATAAAG AATACTTAAC AACTTTATTA 
GAGCATCTAA TGAAGGAATT TTTAGATAAG AAGAGATATC ACAATGACCC AAGGTTCATC 
AATTATTGTC TACAATTTGC CAAGTGCAAC AGTGACCTTC ACCAGTTTTT TGAGTTTCTG 
TACAACCACG GTATCGGAAC CCAAGCAGCT CCTCTGTACG TAGCCTGGGC CGGGCATCTG 
GAAGCCCAGG GGGAGCGGCA GCACGCCAAT GCCGTTTTTC GGAGAGGAAT TCAGAACCAG 
GCCGAACCTA GAGAGCTGCT GCAACAACAG TACAGGATGT TTCAGACACG CCTCACTGAA 
ACCCATTTGC CTACTCAAGT AAGAACCTCA GAACCTCTGC ATGATGCTCA GATTTTAAAT 
CAAATGGTAA CATCAAGCTC AAATCCAGGA AGTAACCCAG CCTGCATTTC TAAGAATCAG 
GGTCCACAAC TTTCTGGAGC AACACCTGCA GCTGGTGATA AAGGGTCGGA CACGGAACAA 
AGGGTGATCA CGATTTCCAA ATCAGAATAT TCTGCACACC CGTCTGTGGC AGACAAAGCT 
GATGTTCAGC AGGTTGTCAT GTATTGCAAG GAGAAGCTTA TTCGCGGAGA ATCGGAGTTT 
TCCTTTGAAG AGCTGAGAGC CCAGAAATAC AATCTACGGA GAAAGCATGA ACAATGGGTA 
AATGAAGAGA GGCATTATAT GAAAAGGAAA GAAGCAAATG CTTTGGAAGA ACAACTATTA 
AAACAGAAAA TGGATGAGCT TCATAAGAAG TTGCACCAAG TGGTGGAGAT GTCGCAGGAG 
AACCTGCCCT CTTCCCAGGG GACATCTGAG GTCAGCCCAG CATGTTTGGA GCCGCATACA 
GACTCCCAGC AGGAACTAAG AGCTCCGGGT CTTCCAGCCA TCTCTCAGCA GACCTCGAAG 
AATGTGGACG AGAAACCCAG AGAGGCATCT TCTGTTCCTC GTATGGCGAA TGCTGCCACC 
ACTGCTTTGG TGTCCCCAGC CGCCAACCAG GGTGTAGCTT GCCCGGCTCC TTTGGCAGGC 
CAGCACGTGA CAGATGCCGT GTTTTCAGGG GGCAGCAGAG ATGCCAGGTG TGTGAACAAA 
AGTACTCACG AATTCAGGCC ACAGAGTGGA GCAGAGTTAA AAGAAGGGTG TGAAACACAG 
AAGGTTGTTA ACACGAGTTC CTTTCACACA ACTCCAAACA CATCTCTGGG ACTGGTTCAG 
GCAACACCAT CCAAAGCGCA GCCATCACCC ACTGTGCACA CGAAGGAGGC TCTCGGTTTC 
ATCATGAATA TGTTTCAGGC TCCTACACTT CCTGATATTT CTGATGACAA AGAGGAATGG 
CCATCTCTAA GTCAAAATGA GTTCGAAGCC CAGTTTCAAA AAAATGCACC ATCTTCTGGG 
GCTTGGGGAG TCAATAAGAT CATCTCTTCT TTGTCATCTG CTTTTCCTGT ATTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CTGAAAAATA AACCTGCAGG AGCCAGGACC 
TTTGGAGAAC GTTCCGTGAG CAGGTTTCCT GCAAAACCAA ATGAAGTGCC TCACACTGAC 
GAGTTTCTGG ATGATTCAAC CGTGTGGGGT GTCCGCTGCA ACAAAAGCCT GGCGCCCAGC 
CCCAAGAGCC CAGGAGACTT TAGGTCCGCC GCGCAGCTGG CGTCTACACC CTTCCACAGG 
CTTCCCGCGG ACTGCGTGCA CCCACCAGAA GATAAAGAAA ATATGGTAGC AAACCAGCAC 
ACCCGTGTGG CTTTGGATTC TTGTGAAAGA AACAAGGTGG AACCCTTGAA AGAGAGAAAG 
TGCAGTCCAG TTGAAGAGAC AAGCCCAGAC CCAGTGTGCC CCGCAGATGG CCCTTCGGCA 
TCCTTGCTCC ATCTGGGCCA ACCTGCTGTA GCCGTGCTGA TGAGTGAGGC AGAGCAGGGT 
CTCAAGGCCT GTAAAGTCAC AGACCCCGAC TTTGCCACTG CAGAGGATCC GGCAGAAGCC 
AGTGCTGAGC TCCAGGCTGA ACTGATGCCG GCTGGCCCGC TTGGGAATGT CGACGCTCCA 
AACTTCACTG TTGAGAACCC TTGGGATGAG GAGTTGATTC TCAAACTTTT ATCCGGGCTT 
TCTAAACCAG TGAGTTCCTA TCCAAATACT TATGAATGGC CATGTAAGCT TCCAGCCATC 
AAGCCCAAGA CTGAATTCCA ATTAGGTTCT TTGCTCGTCT ACGTTGATCA CTTTATTGGA 
CAAGGGGCTT TCGCCCAAGT CTATGAAGTT ACCCATGGAG ATGTGAACAA CAGCAAAAAT 
AAGGAGAAAT TTGCTTTGAA GGTCCAGAAG CCTCCCAACC TCTGGGAATT CTACATTGGG 
ACCCAGCTGA TGGAAAGACT AAAGCCAAGT ATGCAACACA TGTTTATCAA ATTTTATTCA 
GCCCACCTGT TCCAGAATGG CAGCGTGTTA GTAGGAGACC TCTACAGTTA TGGCACATTA 
TTAAATGCCA TTAATCTCTA TAAAAATACC CCTGAAAAAG TGATGCCTCA GGCACTAGTC 
CTCGCTTTTG CTATCAGAAT GCTCTGCATG ATCCAGCAAG TCCATGACTG TGAAATCATC 
CACGGAGACA TCAAGCCAGA CAATTTCATA CTCGGAAACA GATTTTTGGA ACAAGACAAC 
AAAGATGATG ACTTATCTAA CGGCTTGGTC CTGATTGACC TGGGTCAGAG TATAGACATG 
AAACTTTTTC CCAAAGGAAC TGCATTTACA GGAAAGTGTG AAACTTCTGG TTTCCAGTGT 
ATTGAGATGC TCAGTAACAA GCCATGGAAC TACCAGATTG ATTACTTTGG AGTTGCTGCA 
ACCATATACT GCATGCTTTT TGGCACCTAC ATGAAAGTGA AAAATGAAGG GGGCATCTGG 
AAGCCTGAAG GTCTTTTCAG AAGGCTTCCT CACTTGGACA TGTGGAATGA ATTTTTTCAC 
ATAATGTTGA ATATACCCGA TTGTCATCAT CTTCCATCTT TGGATTTGTT AAGGCAGAAG 
CTAAAGAAAA CATTTCAAGA ACACTATACC AGCAAGATCA AGACCCTGTG TAACAGGCTA 
ATTGTACTGC TCTTAGAATA TAAGCGTTCT CGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808025.1
CDS	342..3560
Translation	MFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLYNHGIGTQAAPLYVAWAGHLEAQGERQHANAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLPTQVRTSEPLHDAQILNQMVTSSSNPGSNPACISKNQGPQLSGATPAAGDKGSDTEQRVITISKSEYSAHPSVADKADVQQVVMYCKEKLIRGESEFSFEELRAQKYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQENLPSSQGTSEVSPACLEPHTDSQQELRAPGLPAISQQTSKNVDEKPREASSVPRMANAATTALVSPAANQGVACPAPLAGQHVTDAVFSGGSRDARCVNKSTHEFRPQSGAELKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKAQPSPTVHTKEALGFIMNMFQAPTLPDISDDKEEWPSLSQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSVSRFPAKPNEVPHTDEFLDDSTVWGVRCNKSLAPSPKSPGDFRSAAQLASTPFHRLPADCVHPPEDKENMVANQHTRVALDSCERNKVEPLKERKCSPVEETSPDPVCPADGPSASLLHLGQPAVAVLMSEAEQGLKACKVTDPDFATAEDPAEASAELQAELMPAGPLGNVDAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWPCKLPAIKPKTEFQLGSLLVYVDHFIGQGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLKPSMQHMFIKFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSNGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLFGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_010809723.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809723.2

ORF Insert Sequence:

ATGTTTGAAG CCCATATGCA GAGCTACAAG GGCGATGACC CACTTGGTGA ATGGGAAAGT 
TACATGCAAT GGGTAGAAGA GAATTTTCCT GAGAATAAAG AATACTTAAC AACTTTATTA 
GAGCATCTAA TGAAGGAATT TTTAGATAAG AAGAGATATC ACAATGACCC AAGGTTCATC 
AATTATTGTC TACAATTTGC CAAGTGCAAC AGTGACCTTC ACCAGTTTTT TGAGTTTCTG 
TACAACCACG GTATCGGAAC CCAAGCAGCT CCTCTGTACG TAGCCTGGGC CGGGCATCTG 
GAAGCCCAGG GGGAGCGGCA GCACGCCAAT GCCGTTTTTC GGAGAGGAAT TCAGAACCAG 
GCCGAACCTA GAGAGCTGCT GCAACAACAG TACAGGATGT TTCAGACACG CCTCACTGAA 
ACCCATTTGC CTACTCAAGT AAGAACCTCA GAACCTCTGC ATGATGCTCA GATTTTAAAT 
CAAATGGTAA CATCAAGCTC AAATCCAGGA AGTAACCCAG CCTGCATTTC TAAGAATCAG 
GGTCCACAAC TTTCTGGAGC AACACCTGCA GCTGGTGATA AAGGGTCGGA CACGGAACAA 
AGGGTGATCA CGATTTCCAA ATCAGAATAT TCTGCACACC CGTCTGTGGC AGACAAAGCT 
GATGTTCAGC AGGTTGTCAT GTATTGCAAG GAGAAGCTTA TTCGCGGAGA ATCGGAGTTT 
TCCTTTGAAG AGCTGAGAGC CCAGAAATAC AATCTACGGA GAAAGCATGA ACAATGGGTA 
AATGAAGAGA GGCATTATAT GAAAAGGAAA GAAGCAAATG CTTTGGAAGA ACAACTATTA 
AAACAGAAAA TGGATGAGCT TCATAAGAAG TTGCACCAAG TGGTGGAGAT GTCGCAGGAG 
AACCTGCCCT CTTCCCAGGG GACATCTGAG GTCAGCCCAG CATGTTTGGA GCCGCATACA 
GACTCCCAGC AGGAACTAAG AGCTCCGGGT CTTCCAGCCA TCTCTCAGCA GACCTCGAAG 
AATGTGGACG AGAAACCCAG AGAGGCATCT TCTGTTCCTC GTATGGCGAA TGCTGCCACC 
ACTGCTTTGG TGTCCCCAGC CGCCAACCAG GGTGTAGCTT GCCCGGCTCC TTTGGCAGGC 
CAGCACGTGA CAGATGCCGT GTTTTCAGGG GGCAGCAGAG ATGCCAGGTG TGTGAACAAA 
AGTACTCACG AATTCAGGCC ACAGAGTGGA GCAGAGTTAA AAGAAGGGTG TGAAACACAG 
AAGGTTGTTA ACACGAGTTC CTTTCACACA ACTCCAAACA CATCTCTGGG ACTGGTTCAG 
GCAACACCAT CCAAAGCGCA GCCATCACCC ACTGTGCACA CGAAGGAGGC TCTCGGTTTC 
ATCATGAATA TGTTTCAGGC TCCTACACTT CCTGATATTT CTGATGACAA AGAGGAATGG 
CCATCTCTAA GTCAAAATGA GTTCGAAGCC CAGTTTCAAA AAAATGCACC ATCTTCTGGG 
GCTTGGGGAG TCAATAAGAT CATCTCTTCT TTGTCATCTG CTTTTCCTGT ATTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CTGAAAAATA AACCTGCAGG AGCCAGGACC 
TTTGGAGAAC GTTCCGTGAG CAGGTTTCCT GCAAAACCAA ATGAAGTGCC TCACACTGAC 
GAGTTTCTGG ATGATTCAAC CGTGTGGGGT GTCCGCTGCA ACAAAAGCCT GGCGCCCAGC 
CCCAAGAGCC CAGGAGACTT TAGGTCCGCC GCGCAGCTGG CGTCTACACC CTTCCACAGG 
CTTCCCGCGG ACTGCGTGCA CCCACCAGAA GATAAAGAAA ATATGGTAGC AAACCAGCAC 
ACCCGTGTGG CTTTGGATTC TTGTGAAAGA AACAAGGTGG AACCCTTGAA AGAGAGAAAG 
TGCAGTCCAG TTGAAGAGAC AAGCCCAGAC CCAGTGTGCC CCGCAGATGG CCCTTCGGCA 
TCCTTGCTCC ATCTGGGCCA ACCTGCTGTA GCCGTGCTGA TGAGTGAGGC AGAGCAGGGT 
CTCAAGGCCT GTAAAGTCAC AGACCCCGAC TTTGCCACTG CAGAGGATCC GGCAGAAGCC 
AGTGCTGAGC TCCAGGCTGA ACTGATGCCG GCTGGCCCGC TTGGGAATGT CGACGCTCCA 
AACTTCACTG TTGAGAACCC TTGGGATGAG GAGTTGATTC TCAAACTTTT ATCCGGGCTT 
TCTAAACCAG TGAGTTCCTA TCCAAATACT TATGAATGGC CATGTAAGCT TCCAGCCATC 
AAGCCCAAGA CTGAATTCCA ATTAGGTTCT TTGCTCGTCT ACGTTGATCA CTTTATTGGA 
CAAGGGGCTT TCGCCCAAGT CTATGAAGTT ACCCATGGAG ATGTGAACAA CAGCAAAAAT 
AAGGAGAAAT TTGCTTTGAA GGTCCAGAAG CCTCCCAACC TCTGGGAATT CTACATTGGG 
ACCCAGCTGA TGGAAAGACT AAAGCCAAGT ATGCAACACA TGTTTATCAA ATTTTATTCA 
GCCCACCTGT TCCAGAATGG CAGCGTGTTA GTAGGAGACC TCTACAGTTA TGGCACATTA 
TTAAATGCCA TTAATCTCTA TAAAAATACC CCTGAAAAAG TGATGCCTCA GGCACTAGTC 
CTCGCTTTTG CTATCAGAAT GCTCTGCATG ATCCAGCAAG TCCATGACTG TGAAATCATC 
CACGGAGACA TCAAGCCAGA CAATTTCATA CTCGGAAACA GATTTTTGGA ACAAGACAAC 
AAAGATGATG ACTTATCTAA CGGCTTGGTC CTGATTGACC TGGGTCAGAG TATAGACATG 
AAACTTTTTC CCAAAGGAAC TGCATTTACA GGAAAGTGTG AAACTTCTGG TTTCCAGTGT 
ATTGAGATGC TCAGTAACAA GCCATGGAAC TACCAGATTG ATTACTTTGG AGTTGCTGCA 
ACCATATACT GCATGCTTTT TGGCACCTAC ATGAAAGTGA AAAATGAAGG GGGCATCTGG 
AAGCCTGAAG GTCTTTTCAG AAGGCTTCCT CACTTGGACA TGTGGAATGA ATTTTTTCAC 
ATAATGTTGA ATATACCCGA TTGTCATCAT CTTCCATCTT TGGATTTGTT AAGGCAGAAG 
CTAAAGAAAA CATTTCAAGA ACACTATACC AGCAAGATCA AGACCCTGTG TAACAGGCTA 
ATTGTACTGC TCTTAGAATA TAAGCGTTCT CGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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BUB1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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