LIG3 cDNA ORF clone, Bos taurus(cattle)

The following LIG3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40694 NM_001038107.2
Latest version!
Bos taurus DNA ligase 3 (LIG3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa29465 XM_005220003.3
Latest version!
Bos taurus DNA ligase 3 (LIG3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49068 XM_010815767.2
Latest version!
Bos taurus DNA ligase 3 (LIG3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa29465 XM_005220003.2 Bos taurus ligase III, DNA, ATP-dependent (LIG3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49068 XM_010815767.1 Bos taurus ligase III, DNA, ATP-dependent (LIG3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40694
    Clone ID Related Accession (Same CDS sequence) NM_001038107.2
    Accession Version NM_001038107.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product DNA ligase 3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC111207.1. On Aug 30, 2012 this sequence version replaced NM_001038107.1. ##Evidence-Data-START## Transcript exon combination :: BC111207.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACAGGA GCGGGCCCCG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT ATGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGGTCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC AGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGAGGCG GCCAGCCAGC AAGCGGAGAG GAAGGAGAGC TGTGCCCACA
    GGCAGGAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001033196.1
    CDS56..2887
    Translation

    Target ORF information:

    RefSeq Version NM_001038107.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 3 (LIG3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACAGGA GCGGGCCCCG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT ATGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGGTCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC AGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGAGGCG GCCAGCCAGC AAGCGGAGAG GAAGGAGAGC TGTGCCCACA
    GGCAGGAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29465
    Clone ID Related Accession (Same CDS sequence) XM_005220003.2 , XM_005220003.3
    Accession Version XM_005220003.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005220003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGGTGCT GCTGGACATC TTCACTGGAG TGCGGCTCTA CCTGCCGCCC
    TCCACGCCAG ACTTCAGCCG CCTCAGACGC TACTTTGTGG CATTCGATGG GGACCTGGTA
    CAGGAATTTG ACGTGGGCTC AGCCACACAT GTGCTGGGTG GCGGGGACAG GAACCCCGAG
    GCCCAGCAGG TCTCCCCCGA GTGGATTTGG GCGTGTATCC GGAAACGGAG GCTGGTGGCT
    CCTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220060.1
    CDS119..3127
    Translation

    Target ORF information:

    RefSeq Version XM_005220003.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase III, DNA, ATP-dependent (LIG3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGGTGCT GCTGGACATC TTCACTGGAG TGCGGCTCTA CCTGCCGCCC
    TCCACGCCAG ACTTCAGCCG CCTCAGACGC TACTTTGTGG CATTCGATGG GGACCTGGTA
    CAGGAATTTG ACGTGGGCTC AGCCACACAT GTGCTGGGTG GCGGGGACAG GAACCCCGAG
    GCCCAGCAGG TCTCCCCCGA GTGGATTTGG GCGTGTATCC GGAAACGGAG GCTGGTGGCT
    CCTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29465
    Clone ID Related Accession (Same CDS sequence) XM_005220003.2 , XM_005220003.3
    Accession Version XM_005220003.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGGTGCT GCTGGACATC TTCACTGGAG TGCGGCTCTA CCTGCCGCCC
    TCCACGCCAG ACTTCAGCCG CCTCAGACGC TACTTTGTGG CATTCGATGG GGACCTGGTA
    CAGGAATTTG ACGTGGGCTC AGCCACACAT GTGCTGGGTG GCGGGGACAG GAACCCCGAG
    GCCCAGCAGG TCTCCCCCGA GTGGATTTGG GCGTGTATCC GGAAACGGAG GCTGGTGGCT
    CCTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220060.1
    CDS152..3160
    Translation

    Target ORF information:

    RefSeq Version XM_005220003.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 3 (LIG3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGGTGCT GCTGGACATC TTCACTGGAG TGCGGCTCTA CCTGCCGCCC
    TCCACGCCAG ACTTCAGCCG CCTCAGACGC TACTTTGTGG CATTCGATGG GGACCTGGTA
    CAGGAATTTG ACGTGGGCTC AGCCACACAT GTGCTGGGTG GCGGGGACAG GAACCCCGAG
    GCCCAGCAGG TCTCCCCCGA GTGGATTTGG GCGTGTATCC GGAAACGGAG GCTGGTGGCT
    CCTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49068
    Clone ID Related Accession (Same CDS sequence) XM_010815767.1 , XM_010815767.2
    Accession Version XM_010815767.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGCTCCT TCCCGAGCGG GGTCAGAGCC AACCGCCCCA GCCCAGCCCA
    GCCCTGACTG CTGTGCGTTT GCAGGTGCTG CTGGACATCT TCACTGGAGT GCGGCTCTAC
    CTGCCGCCCT CCACGCCAGA CTTCAGCCGC CTCAGACGCT ACTTTGTGGC ATTCGATGGG
    GACCTGGTAC AGGAATTTGA CGTGGGCTCA GCCACACATG TGCTGGGTGG CGGGGACAGG
    AACCCCGAGG CCCAGCAGGT CTCCCCCGAG TGGATTTGGG CGTGTATCCG GAAACGGAGG
    CTGGTGGCTC CTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814069.1
    CDS119..3196
    Translation

    Target ORF information:

    RefSeq Version XM_010815767.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase III, DNA, ATP-dependent (LIG3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGCTCCT TCCCGAGCGG GGTCAGAGCC AACCGCCCCA GCCCAGCCCA
    GCCCTGACTG CTGTGCGTTT GCAGGTGCTG CTGGACATCT TCACTGGAGT GCGGCTCTAC
    CTGCCGCCCT CCACGCCAGA CTTCAGCCGC CTCAGACGCT ACTTTGTGGC ATTCGATGGG
    GACCTGGTAC AGGAATTTGA CGTGGGCTCA GCCACACATG TGCTGGGTGG CGGGGACAGG
    AACCCCGAGG CCCAGCAGGT CTCCCCCGAG TGGATTTGGG CGTGTATCCG GAAACGGAGG
    CTGGTGGCTC CTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49068
    Clone ID Related Accession (Same CDS sequence) XM_010815767.1 , XM_010815767.2
    Accession Version XM_010815767.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGCTCCT TCCCGAGCGG GGTCAGAGCC AACCGCCCCA GCCCAGCCCA
    GCCCTGACTG CTGTGCGTTT GCAGGTGCTG CTGGACATCT TCACTGGAGT GCGGCTCTAC
    CTGCCGCCCT CCACGCCAGA CTTCAGCCGC CTCAGACGCT ACTTTGTGGC ATTCGATGGG
    GACCTGGTAC AGGAATTTGA CGTGGGCTCA GCCACACATG TGCTGGGTGG CGGGGACAGG
    AACCCCGAGG CCCAGCAGGT CTCCCCCGAG TGGATTTGGG CGTGTATCCG GAAACGGAGG
    CTGGTGGCTC CTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814069.1
    CDS153..3230
    Translation

    Target ORF information:

    RefSeq Version XM_010815767.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 3 (LIG3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACTTTGG CTTTTAAGAT CCTCTTCCCA CCAACTCTCC GTGTACTCAG GAAAAAAGAA 
    CTGTGCCTAT TCCGAGAACA TCGCCGGCCT GACATAAGAC ACTTTGGCCG GTGGTCAGAG
    ACAGTTTTTT GTGGATACCA CCTCCTCCAG AGAAGAAAGC CTGTTCCGTC CTTCCAGGAA
    AGCAACCTAA AACTACGTGC CACCTGCCTT GTTTACTTGC CAGGGTCGCA TGTGGGACTC
    TGCAGTGGCC CCTGTGAGAT GGCAGAGCAA CGCTTCTGTG TGGACTACGC CAAGCGTGGC
    ACGGCTGGCT GCAAAAAATG CAAGGAAAAG ATTGTCAAGG GCGTATGCCG AATCGGCAAA
    GTGGTGCCCA ATCCCTTTTC AGAGTCGGGG GGCGATATGA AAGAGTGGTA CCACATTAAA
    TGCATGTTTG AGAAACTGGA GCGGGCCCGG GCCACCACAA AAAAAATTGA GGACCTCACC
    GAGCTGGAAG GCTGGGAAGA GCTGGAAGAG AATGAGAAAG AGCAGATAAG TCAGCACATT
    GCAGATCTGT CTTCCAAGGC AACAAGCACA CCAAAGAAGA AAGCTGTTGT CCAGGCGAAG
    TTGACAGCCA CTGGCCAGGT CGCATCTCCA GTGAAAGGTG CCTCATTTGT CACCAATTCC
    AATCCCCGGA AGTTTTCTGG CTTTTCAGCC AAGCCCAACA ACTCTGGGGA AGCCCACTCC
    AGCCCTACCC CTAAGACCCC TAAGACAAGT CTGTCCTCAA GCAAATGTGA CCCGAAACAC
    AAGGATTGTC TGCTGCGTGA GTTTCGGAAG TTGTGTGCCA TGGTGGCTGA AAATCCTAGC
    TACAACACGA AAACCCAGAT CATCCAGGAC TTCCTTCAGA AAGGCTCAGC AGGAGATGGT
    TTCCACGGTG ACGTGTACCT AACAGTGAAG CTGCTGCTGC CGGGTGTTAT TAAGAGTGTT
    TACAACTTGA ATGATAAGCA GATTGTGAAG CTTTTCAGCC GCATTTTTAA CTGCAACTCA
    GATGACATGA CACGAGACCT AGAGCAGGGT GACGTGTCAG AGACAATCAG AGTCTTCTTT
    GAGCAGAGCA AGTCTTTCCC TCCAGCTGCC AAGAGCCTCC TTACCATCCA GGAGGTGGAC
    GAATTCCTCC TGCGGCTCTC CAAGCTCACC AAGGAGGATG AGCAGCAACA GGCCCTGCAG
    GATATCGCCT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TCATCAGGCT GATCAAGCAT
    GACCTGAAGA TGAACTCAGG GGCAAAACAC GTGCTAGATG CGCTAGACCC CAATGCCTAT
    GAAGCCTTCA AAGCCTCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT CCGCAACGAG
    CAGGAGGTGG AGAAGGAGCC AGGCCAGAGA CGAGCTCTGA GTGTCCAGGC CTCTCTGATG
    ACCCCAGTGC AGCCCATGCT GGCTGAGGCC TGCAAGTCCA TCGAGTATGC CATGAAGAAG
    TGTCCAAACG GCATGTTCTC TGAGATCAAG TATGACGGGG AGCGAGTCCA GGTGCATAAG
    AAGGGGGACC ACTTCAGCTA CTTCAGCCGC AGTCTCAAGC CCGTCCTGCC ACACAAGGTG
    GCCCACTTTA AGGACTTCAT CCCCCGGGCT TTCCCTGGGG GCCACAGCAT GATCTTGGAC
    TCTGAGGTGC TCCTGATCGA CAACAGGACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA
    GTGCACAAGA AAGCTGCCTT CCAGGATGCC AATGTCTGCC TGTTTGTTTT TGATTGTATC
    TACTTCAATG ATGTCAGCTT GATGGATAGG CCTCTGTGTG AGCGGCGGAA GCTTCTTCAT
    GACAACATGG TGGAGATTCC CAACCGGATC ATGTTCTCAG AAATGAAACA AGTCACAAAA
    GCTTCCGACT TGGTGGACAT GATCAACAGG GTGATCCAAG AGGGGCTGGA AGGGCTGGTG
    CTGAAGGACG TGAAGGGTAC ATATGAGCCT GGAAAGCGTC ATTGGCTGAA AGTGAAGAAA
    GACTATTTGA ACGAGGGTGC CATGGCCGAC ACAGCTGACC TGGTGGTGCT TGGGGCCTTC
    TACGGGCAAG GGAGCAAAGG TGGCATGATG TCCATCTTCC TCATGGGCTG CTATGACCCG
    AGCAGCCAGA AGTGGTGCAC CGTCACCAAG TGTGCAGGAG GCCACGACGA CGCGACGCTC
    GCCCGCCTGC AGACGGAACT GGACATGGTG AAGATCAGCA AGGATCCCAG CAAGATACCC
    AACTGGCTGA AAATCAATAA GATCTACTAC CCTGACTTCA TCGTTCCAGA CCCAAAGAAA
    GCTGCCGTGT GGGAGATCAC AGGGGCTGAA TTCTCGAAAT CTGAGGCTCA CACAGCTGAT
    GGGATCTCCA TCCGATTCCC TCGCTGCACC CGAATCCGTG ATGATAAGGA CTGGAAGTCC
    GCCACGACCC TCCCCCAACT CAAGGAGCTG TACCAGCTGT CCAAGGAGCG CGCGGCCTTC
    GCCATAACGG CCGGAGACGA GGGGAGCTCC GCCACAGGGG GCAGCAGTGG GGAGAACGAG
    GGCACCTCAG GGCCGGCTGT GCCTCACAAG GCCCCAAGAG CCTCCCCCAA GCAGCCTCCC
    ACCAGCGCCA AGAAGGCTGG GGGCAAGCCG GGCGGCTGCA CCAGCAGAGG TGGCAACCTG
    CCAGCTGCAG AGCCCTCCCC TGTGAGAGCG GGAGTGAAAC GGAAGGCTCC TGATGTGACC
    CCGTGCCAAG CCAAGCTCCT TCCCGAGCGG GGTCAGAGCC AACCGCCCCA GCCCAGCCCA
    GCCCTGACTG CTGTGCGTTT GCAGGTGCTG CTGGACATCT TCACTGGAGT GCGGCTCTAC
    CTGCCGCCCT CCACGCCAGA CTTCAGCCGC CTCAGACGCT ACTTTGTGGC ATTCGATGGG
    GACCTGGTAC AGGAATTTGA CGTGGGCTCA GCCACACATG TGCTGGGTGG CGGGGACAGG
    AACCCCGAGG CCCAGCAGGT CTCCCCCGAG TGGATTTGGG CGTGTATCCG GAAACGGAGG
    CTGGTGGCTC CTTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.