TRIP12 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIP12
Entrez Gene ID	514387
Full Name	thyroid hormone receptor interactor 12
Synonyms	TRIP-12
General protein information	Preferred Names thyroid hormone receptor interactor 12 Names E3 ubiquitin-protein ligase TRIP12 HECT-type E3 ubiquitin transferase TRIP12 TR-interacting protein 12 thyroid receptor-interacting protein 12
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015459460.1	XP_015314946.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_015459471.1	XP_015314957.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_015459479.1	XP_015314965.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_010802409.3	XP_010800711.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_010802410.3	XP_010800712.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001202.1	XP_024856970.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001205.1	XP_024856973.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001231.1	XP_024856999.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_025001255.1	XP_024857023.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_025001283.1	XP_024857051.1	E3 ubiquitin-protein ligase TRIP12 isoform X16
XM_025001288.1	XP_024857056.1	E3 ubiquitin-protein ligase TRIP12 isoform X17
XM_025001328.1	XP_024857096.1	E3 ubiquitin-protein ligase TRIP12 isoform X22
XM_025001187.1	XP_024856955.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001222.1	XP_024856990.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_025001290.1	XP_024857058.1	E3 ubiquitin-protein ligase TRIP12 isoform X18
XM_025001310.1	XP_024857078.1	E3 ubiquitin-protein ligase TRIP12 isoform X20
XM_015459481.1	XP_015314967.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_025001197.1	XP_024856965.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001208.1	XP_024856976.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_025001214.1	XP_024856982.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_025001238.1	XP_024857006.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_025001267.1	XP_024857035.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
XM_025001269.1	XP_024857037.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_025001329.1	XP_024857097.1	E3 ubiquitin-protein ligase TRIP12 isoform X23
XM_025001331.1	XP_024857099.1	E3 ubiquitin-protein ligase TRIP12 isoform X24
XM_010802410.2	XP_010800712.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_010802409.2	XP_010800711.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015459476.1	XP_015314962.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_025001240.1	XP_024857008.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_025001250.1	XP_024857018.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_025001263.1	XP_024857031.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_025001273.1	XP_024857041.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_025001302.1	XP_024857070.1	E3 ubiquitin-protein ligase TRIP12 isoform X19
XM_025001322.1	XP_024857090.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_015459467.1	XP_015314953.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_015459470.1	XP_015314956.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_015459474.1	XP_015314960.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_015459475.1	XP_015314961.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_015459483.1	XP_015314969.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_010802422.2	XP_010800724.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_025001195.1	XP_024856963.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025001235.1	XP_024857003.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_025001243.1	XP_024857011.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_015459483.2	XP_015314969.1	E3 ubiquitin-protein ligase TRIP12 isoform X18
NM_001191203.1	NP_001178132.1	E3 ubiquitin-protein ligase TRIP12

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRIP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227293	XM_025001328.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227294	XM_025001329.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa16788	NM_001191203.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48942	XM_025001331.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48942	XM_010802422.2 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa227283	XM_025001235.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227284	XM_025001238.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227285	XM_025001255.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227286	XM_025001267.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227287	XM_025001269.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227288	XM_025001273.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227289	XM_025001283.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227290	XM_025001302.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227291	XM_025001310.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa227292	XM_025001322.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa16781	XM_025001214.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16781	XM_015459467.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16782	XM_015459470.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16782	XM_025001222.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16785	XM_015459479.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16785	XM_025001263.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16787	XM_025001290.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16787	XM_015459483.2 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_025001197.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_025001205.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_025001195.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_010802410.3 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_025001202.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_010802409.3 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_025001187.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48936	XM_015459460.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48936	XM_025001208.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48937	XM_015459471.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48937	XM_025001231.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48938	XM_015459474.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48938	XM_025001240.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48939	XM_025001243.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48939	XM_015459475.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48940	XM_025001250.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48940	XM_015459476.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48941	XM_015459481.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48941	XM_025001288.1 Latest version!	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa48935	XM_010802410.2	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48935	XM_010802409.2	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16787	XM_015459483.1	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIP12 ( XM_025001328.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857096.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X29, mRNA.

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CloneID	OBa227293
Clone ID Related Accession (Same CDS sequence)	XM_025001328.1
Accession Version	XM_025001328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA AACATCCAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC CAAGAAGCGC 
AGCGAATCCC CGCCTGCCGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGCG CTAGTGCCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAGAT GGCCGACCCT GAAGGCAACC AGGAGACGGT GAACTCTTCA 
GCTGCACGGA CAGACGAGAC TCCCCAAGGA GCAGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTGGGCATGA CCACCTCTGG GGAGAGCGAA TCTGATGACT CCGAGATGGG ACGGTTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCCCCT CACCTGTTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTG 
CAAGGACTAC AAGCAAGCGA CGAAAGCCAG CAGCTCCAAG CCGTTATTGA GATGTGTCAG 
TTACTGGTTA TGGGGAATGA GGAGACGCTG GGAGGGTTTC CTGTCAAGAG TGTAGTTCCA 
GCTTTGATAA CATTGCTGCA GATGGAACAT AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCATCGG CTGTGGTAGT TGATGCAATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCCGA GCAGGCCTTG 
ACGGCCTTGG AGATGTTATC ACGTAGACAC AGTAAAGCCA TTCTCCAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTATCT AGAGTTCTTC AGCATAAATG CCCAAAGAAA CGCGCTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCGGATTCC 
CTCCCATTGC TTACCCAAAG GCTAACCCAC CAGGACAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGTCTAGT GGACAACTTC CAACATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTAAC AAATGTTCAA CAACTGTTGG TAGTAACTCC ACCAATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAATTGTCCA 
ACTTTAGCAG TTCAACTTAT GAAGCAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAGCAG ATTGATCTTG TTCCACGAAG TCCTCAAGAA 
CTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA GGAAGGCATT 
TTTGCAGTTG ATACCATGCT GAAGAAAGGC AATGCACAGA ACACGGATGG GGCCATATGG 
CAGTGGCGGG ATGACCGGGG CCTCTGGCAC CCCTATAACA GGATAGACAG CCGGATCATT 
GAGGCAGCCC ATCAGGTCGG TGAGGATGAG ATAAGCTTGT CCACTCTGGG ACGAGTTTAT 
ACTATTGATT TTAATTCTAT GCAGCAAATC AATGAGGACA CGGGAACAGC ACGTGCCATT 
CAGAGAAAAC CTAACCCCTT AGCCAATACT AACACTAGTG GATATTCAGA GTTAAAGAAG 
GATGATGCTC GAGCACAGCT TATGAAAGAG GATCCGGAAC TGGCTAAGTC TTTTATTAAG 
ACATTATTTG GTGTTCTTTA TGAAGTGTAT AGTTCCTCAG CAGGACCTGC GGTCAGACAT 
AAGTGCCTTA GAGCAATTCT TAGGATAATT TATTTCGCGG ATGCCGAACT TCTAAAGGAT 
GTCCTGAAAA ACCATGCTGT TTCAAGTCAT ATTGCTTCCA TGCTATCAAG CCAAGACCTG 
AAGATAGTAG TTGGAGCCCT TCAAATGGCA GAGATCTTAA TGCAGAAGTT ACCTGATATT 
TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA ATGCATCAAG TAAAACACTT AGCAGAATCT 
GAGTCATTGT TGACAAGTCC ACCAAAGGCA TGTACAAATG GGTCCGGATC CTTGGGGTCC 
ACGCCATCGG TCAACAGTGG AACAGCCACG GCCGCCACCA ATGCCTCGGC TGACCTGGGG 
TCCCCCAGCT TGCAGCACAG CAGAGACGAT TCTCTAGACC TGAGCCCTCA AGGTCGATTA 
AGTGATGTTC TAAAGAGAAA ACGACTGCCA AAACGAGGAT CAAGAAGGCC AAAGTATTCA 
CCTCCGAGAG ATGATGACAA AGTAGACAAT CAAGCTAAAA GCCCCACTAC TACTCAGTCA 
CCTAAGTCTT CTTTCTTGGC AAGCTTGAAT CCAAAAACGT GGGGAAGGTT AAGCGCACAG 
TCTAACAGCA ACAACATTGA ACCAGCACGA ACTGCGGGAG TTAGTGGTCT TGCCAGGGCC 
GCCTCAAAGG ACACCATCTC CAATAATAGA GAAAAAATTA AAGGTTGGAT TAAAGAGCAG 
GCACATAAAT TTGTAGAGCG TTACTTCAGT TCTGAGAACA TGGATGGAAG CAACCCTGCA 
TTGAATGTCC TTCAGAGACT TTGTGCTGCT ACTGAACAAC TCAACCTCCA GGTGGATGGT 
GGAGCTGAGT GCCTTGTAGA AATCCGTAGC ATAGTCTCAG AGTCTGATGT TTCATCGTTT 
GAAATCCAAC ACAGTGGATT TGTGAAGCAA CTGTTGCTTT ATTTGACATC TAAAAGTGAA 
AAAGACGCTG TGAGCAGAGA GATCAGATTA AAGAGATTCC TTCATGTATT TTTTTCTTCT 
CCACTTCCTG GAGAAGAGCC CATTGAACGA GTAGAACCAG TGGGTAATGC ACCTTTGTTG 
GCATTAGTTC ACAAAATGAA CAACTGCCTC AGCCAGATGG AACAATTTCC AGTCAAAGTG 
CATGACTTTC CCAGTGGAAA TGGGACAGGA GGCAGCTTCT CTCTCAACAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACATCAGTTA AAATGCCAGT TACAAAGACA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGTGGACCT GTCAAGATTG ACCCCCTGGC TTTGGTGCAG 
GCTATTGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGACGAAGAC 
AGTGACGACG ATGGTTCTGA CGAGGAAATA GACGAATCTC TGGCTGCTCA GTTCTTAAAT 
TCCGGAAATG TGAGACACAG GCTACAGTTT TACATCGGGG AGCATTTGCT ACCGTATAAC 
ATGACTGTGT ATCAGGCAGT ACGGCAGTTC AGTATACAGG CTGAAGATGA ACGGGAGTCC 
ACGGATGACG AGAGCAATCC TCTAGGAAGA GCTGGTATTT GGACGAAAAC TCATACGATA 
TGGTACAAAC CTGTGCGAGA GGACGAAGAA AGTAATAAAG ATTGTGTTGG TGGTAAAAGA 
GGAAGAGCCC AGACAGCTCC AACAAAAACT TCCCCTAGAA ATGCAAAAAA GCATGATGAG 
CTGTGGAATG ATGGAGTGTG CCCATCCGTA TCAAATCCTT TAGAAGTTTA CCTCATACCC 
ACAGCACCCG AAAATATAAC TTTTGAAGAC CCGTCATTAG ATGTAATTCT TCTTTTAAGA 
GTTTTACACG CCGTCAGTAG ATACTGGTAT TACTTGTATG ATAATGCAAT GTGCAAGGAG 
ATTATCCCAA CTAGTGAATT TATTAACAGT AAGTTGACAG CAAAAGCAAA TAGGCAGCTT 
CAAGATCCTT TGGTAATCAT GACAGGAAAC ATCCCTACAT GGCTCACTGA ACTAGGAAAA 
ACCTGCCCAT TTTTCTTCCC TTTTGATACC CGGCAAATGC TTTTCTATGT AACTGCATTT 
GATCGGGACC GAGCAATGCA AAGATTACTT GATACCAACC CAGAGATCAA CCAGTCTGAT 
TCTCAAGATA GCAGAGTTGC ACCTAGATTG GACAGAAAAA AACGTACTGT GAACAGAGAG 
GAGCTGCTGA AACAAGCGGA GTCTGTGATG CAGGATCTTG GCAGCTCACG GGCCATGTTA 
GAAATCCAGT ATGAAAATGA GGTTGGCACA GGTCTTGGGC CTACACTGGA GTTTTATGCA 
CTTGTATCTC AGGAACTGCA GAGAGCTGAC TTGGGTCTCT GGAGAGGGGA AGAAGTAACT 
CTTAGCAATC CAAAAGGAAG CCAAGAAGGG ACCAAGTATA TTCAAAACCT CCAAGGCCTG 
TTTGCACTTC CCTTTGGTAG GACAGCTAAG CCAGCTCATA TCGCAAAGGT TAAAATGAAG 
TTTCGCTTCT TAGGAAAACT AATGGCCAAG GCTATCATGG ATTTCAGATT GGTGGACCTT 
CCCCTGGGCT TACCTTTTTA TAAATGGATG CTCCGGCAAG AAACTTCGCT GACGTCACAC 
GATTTGTTTG ACATTGACCC AGTTGTAGCC AGGTCAGTGT ATCACCTCGA AGACATCGTC 
AGACAGAAGA AAAGACTTGA ACAAGATAAA TCCCAGACCA AAGAGAGTCT CCAGTATGCA 
TTAGAAACCC TGACTATGAA TGGCTGCTCC GTGGAGGATC TGGGATTGGA TTTCACTCTG 
CCAGGGTTTC CTAACATTGA ACTAAAGAAA GGAGGAAAGG ATATACCGGT CACCATCCAC 
AATTTGGAGG AGTATCTAAG ACTGGTTATA TTTTGGGCAC TAAATGAAGG TGTTTCCAGA 
CAATTTGATT CATTTAGAGA TGGATTTGAA TCAGTCTTCC CACTCAGTCA TCTCCAGTAC 
TTCTACCCAG AAGAACTGGA TCAGCTTCTG TGTGGTAGTA AAGCAGACAC TTGGGATGCA 
AAGACACTGA TGGAATGCTG CAGGCCAGAT CACGGTTACA CTCATGACAG TCGGGCTGTG 
AAGTTCTTGT TTGAGATTCT CAGTAGTTTT GACAATGAAC AGCAGAGGTT ATTTCTCCAG 
TTTGTGACAG GTAGCCCACG ATTGCCTGTT GGAGGCTTCC GGAGCTTGAA TCCACCTTTG 
ACCATTGTCC GGAAGACCTT CGAATCAACA GAGAACCCAG ATGACTTCCT ACCCTCTGTC 
ATGACCTGTG TGAACTATCT TAAGTTGCCG GACTATTCAA GCCTCGAGAT AATGCGGGAA 
AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857096.1
CDS	290..6280
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001328.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001328.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA AACATCCAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC CAAGAAGCGC 
AGCGAATCCC CGCCTGCCGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGCG CTAGTGCCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAGAT GGCCGACCCT GAAGGCAACC AGGAGACGGT GAACTCTTCA 
GCTGCACGGA CAGACGAGAC TCCCCAAGGA GCAGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTGGGCATGA CCACCTCTGG GGAGAGCGAA TCTGATGACT CCGAGATGGG ACGGTTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCCCCT CACCTGTTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTG 
CAAGGACTAC AAGCAAGCGA CGAAAGCCAG CAGCTCCAAG CCGTTATTGA GATGTGTCAG 
TTACTGGTTA TGGGGAATGA GGAGACGCTG GGAGGGTTTC CTGTCAAGAG TGTAGTTCCA 
GCTTTGATAA CATTGCTGCA GATGGAACAT AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCATCGG CTGTGGTAGT TGATGCAATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCCGA GCAGGCCTTG 
ACGGCCTTGG AGATGTTATC ACGTAGACAC AGTAAAGCCA TTCTCCAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTATCT AGAGTTCTTC AGCATAAATG CCCAAAGAAA CGCGCTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCGGATTCC 
CTCCCATTGC TTACCCAAAG GCTAACCCAC CAGGACAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGTCTAGT GGACAACTTC CAACATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTAAC AAATGTTCAA CAACTGTTGG TAGTAACTCC ACCAATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAATTGTCCA 
ACTTTAGCAG TTCAACTTAT GAAGCAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAGCAG ATTGATCTTG TTCCACGAAG TCCTCAAGAA 
CTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA GGAAGGCATT 
TTTGCAGTTG ATACCATGCT GAAGAAAGGC AATGCACAGA ACACGGATGG GGCCATATGG 
CAGTGGCGGG ATGACCGGGG CCTCTGGCAC CCCTATAACA GGATAGACAG CCGGATCATT 
GAGGCAGCCC ATCAGGTCGG TGAGGATGAG ATAAGCTTGT CCACTCTGGG ACGAGTTTAT 
ACTATTGATT TTAATTCTAT GCAGCAAATC AATGAGGACA CGGGAACAGC ACGTGCCATT 
CAGAGAAAAC CTAACCCCTT AGCCAATACT AACACTAGTG GATATTCAGA GTTAAAGAAG 
GATGATGCTC GAGCACAGCT TATGAAAGAG GATCCGGAAC TGGCTAAGTC TTTTATTAAG 
ACATTATTTG GTGTTCTTTA TGAAGTGTAT AGTTCCTCAG CAGGACCTGC GGTCAGACAT 
AAGTGCCTTA GAGCAATTCT TAGGATAATT TATTTCGCGG ATGCCGAACT TCTAAAGGAT 
GTCCTGAAAA ACCATGCTGT TTCAAGTCAT ATTGCTTCCA TGCTATCAAG CCAAGACCTG 
AAGATAGTAG TTGGAGCCCT TCAAATGGCA GAGATCTTAA TGCAGAAGTT ACCTGATATT 
TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA ATGCATCAAG TAAAACACTT AGCAGAATCT 
GAGTCATTGT TGACAAGTCC ACCAAAGGCA TGTACAAATG GGTCCGGATC CTTGGGGTCC 
ACGCCATCGG TCAACAGTGG AACAGCCACG GCCGCCACCA ATGCCTCGGC TGACCTGGGG 
TCCCCCAGCT TGCAGCACAG CAGAGACGAT TCTCTAGACC TGAGCCCTCA AGGTCGATTA 
AGTGATGTTC TAAAGAGAAA ACGACTGCCA AAACGAGGAT CAAGAAGGCC AAAGTATTCA 
CCTCCGAGAG ATGATGACAA AGTAGACAAT CAAGCTAAAA GCCCCACTAC TACTCAGTCA 
CCTAAGTCTT CTTTCTTGGC AAGCTTGAAT CCAAAAACGT GGGGAAGGTT AAGCGCACAG 
TCTAACAGCA ACAACATTGA ACCAGCACGA ACTGCGGGAG TTAGTGGTCT TGCCAGGGCC 
GCCTCAAAGG ACACCATCTC CAATAATAGA GAAAAAATTA AAGGTTGGAT TAAAGAGCAG 
GCACATAAAT TTGTAGAGCG TTACTTCAGT TCTGAGAACA TGGATGGAAG CAACCCTGCA 
TTGAATGTCC TTCAGAGACT TTGTGCTGCT ACTGAACAAC TCAACCTCCA GGTGGATGGT 
GGAGCTGAGT GCCTTGTAGA AATCCGTAGC ATAGTCTCAG AGTCTGATGT TTCATCGTTT 
GAAATCCAAC ACAGTGGATT TGTGAAGCAA CTGTTGCTTT ATTTGACATC TAAAAGTGAA 
AAAGACGCTG TGAGCAGAGA GATCAGATTA AAGAGATTCC TTCATGTATT TTTTTCTTCT 
CCACTTCCTG GAGAAGAGCC CATTGAACGA GTAGAACCAG TGGGTAATGC ACCTTTGTTG 
GCATTAGTTC ACAAAATGAA CAACTGCCTC AGCCAGATGG AACAATTTCC AGTCAAAGTG 
CATGACTTTC CCAGTGGAAA TGGGACAGGA GGCAGCTTCT CTCTCAACAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACATCAGTTA AAATGCCAGT TACAAAGACA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGTGGACCT GTCAAGATTG ACCCCCTGGC TTTGGTGCAG 
GCTATTGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGACGAAGAC 
AGTGACGACG ATGGTTCTGA CGAGGAAATA GACGAATCTC TGGCTGCTCA GTTCTTAAAT 
TCCGGAAATG TGAGACACAG GCTACAGTTT TACATCGGGG AGCATTTGCT ACCGTATAAC 
ATGACTGTGT ATCAGGCAGT ACGGCAGTTC AGTATACAGG CTGAAGATGA ACGGGAGTCC 
ACGGATGACG AGAGCAATCC TCTAGGAAGA GCTGGTATTT GGACGAAAAC TCATACGATA 
TGGTACAAAC CTGTGCGAGA GGACGAAGAA AGTAATAAAG ATTGTGTTGG TGGTAAAAGA 
GGAAGAGCCC AGACAGCTCC AACAAAAACT TCCCCTAGAA ATGCAAAAAA GCATGATGAG 
CTGTGGAATG ATGGAGTGTG CCCATCCGTA TCAAATCCTT TAGAAGTTTA CCTCATACCC 
ACAGCACCCG AAAATATAAC TTTTGAAGAC CCGTCATTAG ATGTAATTCT TCTTTTAAGA 
GTTTTACACG CCGTCAGTAG ATACTGGTAT TACTTGTATG ATAATGCAAT GTGCAAGGAG 
ATTATCCCAA CTAGTGAATT TATTAACAGT AAGTTGACAG CAAAAGCAAA TAGGCAGCTT 
CAAGATCCTT TGGTAATCAT GACAGGAAAC ATCCCTACAT GGCTCACTGA ACTAGGAAAA 
ACCTGCCCAT TTTTCTTCCC TTTTGATACC CGGCAAATGC TTTTCTATGT AACTGCATTT 
GATCGGGACC GAGCAATGCA AAGATTACTT GATACCAACC CAGAGATCAA CCAGTCTGAT 
TCTCAAGATA GCAGAGTTGC ACCTAGATTG GACAGAAAAA AACGTACTGT GAACAGAGAG 
GAGCTGCTGA AACAAGCGGA GTCTGTGATG CAGGATCTTG GCAGCTCACG GGCCATGTTA 
GAAATCCAGT ATGAAAATGA GGTTGGCACA GGTCTTGGGC CTACACTGGA GTTTTATGCA 
CTTGTATCTC AGGAACTGCA GAGAGCTGAC TTGGGTCTCT GGAGAGGGGA AGAAGTAACT 
CTTAGCAATC CAAAAGGAAG CCAAGAAGGG ACCAAGTATA TTCAAAACCT CCAAGGCCTG 
TTTGCACTTC CCTTTGGTAG GACAGCTAAG CCAGCTCATA TCGCAAAGGT TAAAATGAAG 
TTTCGCTTCT TAGGAAAACT AATGGCCAAG GCTATCATGG ATTTCAGATT GGTGGACCTT 
CCCCTGGGCT TACCTTTTTA TAAATGGATG CTCCGGCAAG AAACTTCGCT GACGTCACAC 
GATTTGTTTG ACATTGACCC AGTTGTAGCC AGGTCAGTGT ATCACCTCGA AGACATCGTC 
AGACAGAAGA AAAGACTTGA ACAAGATAAA TCCCAGACCA AAGAGAGTCT CCAGTATGCA 
TTAGAAACCC TGACTATGAA TGGCTGCTCC GTGGAGGATC TGGGATTGGA TTTCACTCTG 
CCAGGGTTTC CTAACATTGA ACTAAAGAAA GGAGGAAAGG ATATACCGGT CACCATCCAC 
AATTTGGAGG AGTATCTAAG ACTGGTTATA TTTTGGGCAC TAAATGAAGG TGTTTCCAGA 
CAATTTGATT CATTTAGAGA TGGATTTGAA TCAGTCTTCC CACTCAGTCA TCTCCAGTAC 
TTCTACCCAG AAGAACTGGA TCAGCTTCTG TGTGGTAGTA AAGCAGACAC TTGGGATGCA 
AAGACACTGA TGGAATGCTG CAGGCCAGAT CACGGTTACA CTCATGACAG TCGGGCTGTG 
AAGTTCTTGT TTGAGATTCT CAGTAGTTTT GACAATGAAC AGCAGAGGTT ATTTCTCCAG 
TTTGTGACAG GTAGCCCACG ATTGCCTGTT GGAGGCTTCC GGAGCTTGAA TCCACCTTTG 
ACCATTGTCC GGAAGACCTT CGAATCAACA GAGAACCCAG ATGACTTCCT ACCCTCTGTC 
ATGACCTGTG TGAACTATCT TAAGTTGCCG GACTATTCAA GCCTCGAGAT AATGCGGGAA 
AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001329.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857097.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.

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CloneID	OBa227294
Clone ID Related Accession (Same CDS sequence)	XM_025001329.1
Accession Version	XM_025001329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGTTCTG ACGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGG GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGACGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCATCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA CGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TCCAGTATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAATGAAG GTGTTTCCAG ACAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GATTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTCGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857097.1
CDS	291..6272
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001329.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001329.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGTTCTG ACGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGG GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGACGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCATCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA CGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TCCAGTATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAATGAAG GTGTTTCCAG ACAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GATTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTCGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001191203.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178132.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), mRNA.

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CloneID	OBa16788
Clone ID Related Accession (Same CDS sequence)	NM_001191203.1
Accession Version	NM_001191203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_001252754.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGGGGCTG TGGGCATGAC CACCTCTGGG GAGAGCGAAT CTGATGACTC CGAGATGGGA 
CGGTTACAAG CTCTGTTAGA GGCCAGGGGT CTTCCCCCTC ACCTGTTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTGC AAGGACTACA AGCAAGCGAC GAAAGCCAGC AGCTCCAAGC CGTTATTGAG 
ATGTGTCAGT TACTGGTTAT GGGGAATGAG GAGACGCTGG GAGGGTTTCC TGTCAAGAGT 
GTAGTTCCAG CTTTGATAAC ATTGCTGCAG ATGGAACATA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCATCGGC TGTGGTAGTT 
GATGCAATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCCGAG 
CAGGCCTTGA CGGCCTTGGA GATGTTATCA CGTAGACACA GTAAAGCCAT TCTCCAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTATCTA GAGTTCTTCA GCATAAATGC CCAAAGAAAC 
GCGCTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCGGATTCCC TCCCATTGCT TACCCAAAGG CTAACCCACC AGGACAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGTCTAGTG GACAACTTCC AACATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTAACA AATGTTCAAC AACTGTTGGT AGTAACTCCA 
CCAATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AATTGTCCAA CTTTAGCAGT TCAACTTATG AAGCAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAAGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCCTTAG CCAATACTAA CACTAGTGGA TATTCAGAGT TAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTCGCGGAT GCCGAACTTC TAAAGGATGT CCTGAAAAAC 
CATGCTGTTT CAAGTCATAT TGCTTCCATG CTATCAAGCC AAGACCTGAA GATAGTAGTT 
GGAGCCCTTC AAATGGCAGA GATCTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCTGA GTCATTGTTG 
ACAAGTCCAC CAAAGGCATG TACAAATGGG TCCGGATCCT TGGGGTCCAC GCCATCGGTC 
AACAGTGGAA CAGCCACGGC CGCCACCAAT GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG 
CAGCACAGCA GAGACGATTC TCTAGACCTG AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGATCA AGAAGGCCAA AGTATTCACC TCCGAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACTACTA CTCAGTCACC TAAGTCTTCT 
TTCTTGGCAA GCTTGAATCC AAAAACGTGG GGAAGGTTAA GCGCACAGTC TAACAGCAAC 
AACATTGAAC CAGCACGAAC TGCGGGAGTT AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AAGAGCAGGC ACATAAATTT 
GTAGAGCGTT ACTTCAGTTC TGAGAACATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCTAC TGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCTGATGTTT CATCGTTTGA AATCCAACAC 
AGTGGATTTG TGAAGCAACT GTTGCTTTAT TTGACATCTA AAAGTGAAAA AGACGCTGTG 
AGCAGAGAGA TCAGATTAAA GAGATTCCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGAACGAGT AGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAAATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTGCA TGACTTTCCC 
AGTGGAAATG GGACAGGAGG CAGCTTCTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAGTTAAA ATGCCAGTTA CAAAGACATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCCCTGGCTT TGGTGCAGGC TATTGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ACGAAGACAG TGACGACGAT 
GGTTCTGACG AGGAAATAGA CGAATCTCTG GCTGCTCAGT TCTTAAATTC CGGAAATGTG 
AGACACAGGC TACAGTTTTA CATCGGGGAG CATTTGCTAC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTCAG TATACAGGCT GAAGATGAAC GGGAGTCCAC GGATGACGAG 
AGCAATCCTC TAGGAAGAGC TGGTATTTGG ACGAAAACTC ATACGATATG GTACAAACCT 
GTGCGAGAGG ACGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG 
ACAGCTCCAA CAAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGCT GTGGAATGAT 
GGAGTGTGCC CATCCGTATC AAATCCTTTA GAAGTTTACC TCATACCCAC AGCACCCGAA 
AATATAACTT TTGAAGACCC GTCATTAGAT GTAATTCTTC TTTTAAGAGT TTTACACGCC 
GTCAGTAGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAGAT TATCCCAACT 
AGTGAATTTA TTAACAGTAA GTTGACAGCA AAAGCAAATA GGCAGCTTCA AGATCCTTTG 
GTAATCATGA CAGGAAACAT CCCTACATGG CTCACTGAAC TAGGAAAAAC CTGCCCATTT 
TTCTTCCCTT TTGATACCCG GCAAATGCTT TTCTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAGATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA CAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTGCTGAAA 
CAAGCGGAGT CTGTGATGCA GGATCTTGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGCACAGG TCTTGGGCCT ACACTGGAGT TTTATGCACT TGTATCTCAG 
GAACTGCAGA GAGCTGACTT GGGTCTCTGG AGAGGGGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AAGGCCTGTT TGCACTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCATATC GCAAAGGTTA AAATGAAGTT TCGCTTCTTA 
GGAAAACTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTGGGCTTA 
CCTTTTTATA AATGGATGCT CCGGCAAGAA ACTTCGCTGA CGTCACACGA TTTGTTTGAC 
ATTGACCCAG TTGTAGCCAG GTCAGTGTAT CACCTCGAAG ACATCGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTCC AGTATGCATT AGAAACCCTG 
ACTATGAATG GCTGCTCCGT GGAGGATCTG GGATTGGATT TCACTCTGCC AGGGTTTCCT 
AACATTGAAC TAAAGAAAGG AGGAAAGGAT ATACCGGTCA CCATCCACAA TTTGGAGGAG 
TATCTAAGAC TGGTTATATT TTGGGCACTA AATGAAGGTG TTTCCAGACA ATTTGATTCA 
TTTAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TCCAGTACTT CTACCCAGAA 
GAACTGGATC AGCTTCTGTG TGGTAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGCA GGCCAGATCA CGGTTACACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA CAATGAACAG CAGAGGTTAT TTCTCCAGTT TGTGACAGGT 
AGCCCACGAT TGCCTGTTGG AGGCTTCCGG AGCTTGAATC CACCTTTGAC CATTGTCCGG 
AAGACCTTCG AATCAACAGA GAACCCAGAT GACTTCCTAC CCTCTGTCAT GACCTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC CTCGAGATAA TGCGGGAAAA GCTGTTGATG 
GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178132.1
CDS	1..5979
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001191203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191203.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGGGGCTG TGGGCATGAC CACCTCTGGG GAGAGCGAAT CTGATGACTC CGAGATGGGA 
CGGTTACAAG CTCTGTTAGA GGCCAGGGGT CTTCCCCCTC ACCTGTTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTGC AAGGACTACA AGCAAGCGAC GAAAGCCAGC AGCTCCAAGC CGTTATTGAG 
ATGTGTCAGT TACTGGTTAT GGGGAATGAG GAGACGCTGG GAGGGTTTCC TGTCAAGAGT 
GTAGTTCCAG CTTTGATAAC ATTGCTGCAG ATGGAACATA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCATCGGC TGTGGTAGTT 
GATGCAATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCCGAG 
CAGGCCTTGA CGGCCTTGGA GATGTTATCA CGTAGACACA GTAAAGCCAT TCTCCAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTATCTA GAGTTCTTCA GCATAAATGC CCAAAGAAAC 
GCGCTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCGGATTCCC TCCCATTGCT TACCCAAAGG CTAACCCACC AGGACAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGTCTAGTG GACAACTTCC AACATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTAACA AATGTTCAAC AACTGTTGGT AGTAACTCCA 
CCAATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AATTGTCCAA CTTTAGCAGT TCAACTTATG AAGCAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAAGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCCTTAG CCAATACTAA CACTAGTGGA TATTCAGAGT TAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTCGCGGAT GCCGAACTTC TAAAGGATGT CCTGAAAAAC 
CATGCTGTTT CAAGTCATAT TGCTTCCATG CTATCAAGCC AAGACCTGAA GATAGTAGTT 
GGAGCCCTTC AAATGGCAGA GATCTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCTGA GTCATTGTTG 
ACAAGTCCAC CAAAGGCATG TACAAATGGG TCCGGATCCT TGGGGTCCAC GCCATCGGTC 
AACAGTGGAA CAGCCACGGC CGCCACCAAT GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG 
CAGCACAGCA GAGACGATTC TCTAGACCTG AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGATCA AGAAGGCCAA AGTATTCACC TCCGAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACTACTA CTCAGTCACC TAAGTCTTCT 
TTCTTGGCAA GCTTGAATCC AAAAACGTGG GGAAGGTTAA GCGCACAGTC TAACAGCAAC 
AACATTGAAC CAGCACGAAC TGCGGGAGTT AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AAGAGCAGGC ACATAAATTT 
GTAGAGCGTT ACTTCAGTTC TGAGAACATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCTAC TGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCTGATGTTT CATCGTTTGA AATCCAACAC 
AGTGGATTTG TGAAGCAACT GTTGCTTTAT TTGACATCTA AAAGTGAAAA AGACGCTGTG 
AGCAGAGAGA TCAGATTAAA GAGATTCCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGAACGAGT AGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAAATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTGCA TGACTTTCCC 
AGTGGAAATG GGACAGGAGG CAGCTTCTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAGTTAAA ATGCCAGTTA CAAAGACATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCCCTGGCTT TGGTGCAGGC TATTGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ACGAAGACAG TGACGACGAT 
GGTTCTGACG AGGAAATAGA CGAATCTCTG GCTGCTCAGT TCTTAAATTC CGGAAATGTG 
AGACACAGGC TACAGTTTTA CATCGGGGAG CATTTGCTAC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTCAG TATACAGGCT GAAGATGAAC GGGAGTCCAC GGATGACGAG 
AGCAATCCTC TAGGAAGAGC TGGTATTTGG ACGAAAACTC ATACGATATG GTACAAACCT 
GTGCGAGAGG ACGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG 
ACAGCTCCAA CAAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGCT GTGGAATGAT 
GGAGTGTGCC CATCCGTATC AAATCCTTTA GAAGTTTACC TCATACCCAC AGCACCCGAA 
AATATAACTT TTGAAGACCC GTCATTAGAT GTAATTCTTC TTTTAAGAGT TTTACACGCC 
GTCAGTAGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAGAT TATCCCAACT 
AGTGAATTTA TTAACAGTAA GTTGACAGCA AAAGCAAATA GGCAGCTTCA AGATCCTTTG 
GTAATCATGA CAGGAAACAT CCCTACATGG CTCACTGAAC TAGGAAAAAC CTGCCCATTT 
TTCTTCCCTT TTGATACCCG GCAAATGCTT TTCTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAGATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA CAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTGCTGAAA 
CAAGCGGAGT CTGTGATGCA GGATCTTGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGCACAGG TCTTGGGCCT ACACTGGAGT TTTATGCACT TGTATCTCAG 
GAACTGCAGA GAGCTGACTT GGGTCTCTGG AGAGGGGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AAGGCCTGTT TGCACTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCATATC GCAAAGGTTA AAATGAAGTT TCGCTTCTTA 
GGAAAACTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTGGGCTTA 
CCTTTTTATA AATGGATGCT CCGGCAAGAA ACTTCGCTGA CGTCACACGA TTTGTTTGAC 
ATTGACCCAG TTGTAGCCAG GTCAGTGTAT CACCTCGAAG ACATCGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTCC AGTATGCATT AGAAACCCTG 
ACTATGAATG GCTGCTCCGT GGAGGATCTG GGATTGGATT TCACTCTGCC AGGGTTTCCT 
AACATTGAAC TAAAGAAAGG AGGAAAGGAT ATACCGGTCA CCATCCACAA TTTGGAGGAG 
TATCTAAGAC TGGTTATATT TTGGGCACTA AATGAAGGTG TTTCCAGACA ATTTGATTCA 
TTTAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TCCAGTACTT CTACCCAGAA 
GAACTGGATC AGCTTCTGTG TGGTAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGCA GGCCAGATCA CGGTTACACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA CAATGAACAG CAGAGGTTAT TTCTCCAGTT TGTGACAGGT 
AGCCCACGAT TGCCTGTTGG AGGCTTCCGG AGCTTGAATC CACCTTTGAC CATTGTCCGG 
AAGACCTTCG AATCAACAGA GAACCCAGAT GACTTCCTAC CCTCTGTCAT GACCTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC CTCGAGATAA TGCGGGAAAA GCTGTTGATG 
GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001331.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857099.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.

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CloneID	OBa48942
Clone ID Related Accession (Same CDS sequence)	XM_025001331.1 , XM_010802422.2
Accession Version	XM_025001331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857099.1
CDS	290..6286
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001331.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001331.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_010802422.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800724.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

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CloneID	OBa48942
Clone ID Related Accession (Same CDS sequence)	XM_025001331.1 , XM_010802422.2
Accession Version	XM_010802422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800724.1
CDS	1718..7714
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_010802422.2
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802422.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001235.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857003.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OBa227283
Clone ID Related Accession (Same CDS sequence)	XM_025001235.1
Accession Version	XM_025001235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857003.1
CDS	292..6483
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001235.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001235.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001238.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857006.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

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CloneID	OBa227284
Clone ID Related Accession (Same CDS sequence)	XM_025001238.1
Accession Version	XM_025001238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857006.1
CDS	292..6480
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001238.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001238.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001255.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857023.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

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CloneID	OBa227285
Clone ID Related Accession (Same CDS sequence)	XM_025001255.1
Accession Version	XM_025001255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT CGCGGATGCC 
GAACTTCTAA AGGATGTCCT GAAAAACCAT GCTGTTTCAA GTCATATTGC TTCCATGCTA 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCCCTTCAAA TGGCAGAGAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCTGAGTC ATTGTTGACA AGTCCACCAA AGGCATGTAC AAATGGGTCC 
GGATCCTTGG GGTCCACGCC ATCGGTCAAC AGTGGAACAG CCACGGCCGC CACCAATGCC 
TCGGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ACGATTCTCT AGACCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGATCAAGA 
AGGCCAAAGT ATTCACCTCC GAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACTACTACTC AGTCACCTAA GTCTTCTTTC TTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCTAA CAGCAACAAC ATTGAACCAG CACGAACTGC GGGAGTTAGT 
GGTCTTGCCA GGGCCGCCTC AAAGGACACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAAG AGCAGGCACA TAAATTTGTA GAGCGTTACT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCTACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CGTTTGAAAT CCAACACAGT GGATTTGTGA AGCAACTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAAGA CGCTGTGAGC AGAGAGATCA GATTAAAGAG ATTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG AACGAGTAGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAA ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTGCATGA CTTTCCCAGT GGAAATGGGA CAGGAGGCAG CTTCTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGT TCTGACGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGGGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGACG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCAT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACACGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTCCAGT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAT 
GAAGGTGTTT CCAGACAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGATTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTC 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCGAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857023.1
CDS	291..6416
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001255.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001255.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT CGCGGATGCC 
GAACTTCTAA AGGATGTCCT GAAAAACCAT GCTGTTTCAA GTCATATTGC TTCCATGCTA 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCCCTTCAAA TGGCAGAGAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCTGAGTC ATTGTTGACA AGTCCACCAA AGGCATGTAC AAATGGGTCC 
GGATCCTTGG GGTCCACGCC ATCGGTCAAC AGTGGAACAG CCACGGCCGC CACCAATGCC 
TCGGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ACGATTCTCT AGACCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGATCAAGA 
AGGCCAAAGT ATTCACCTCC GAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACTACTACTC AGTCACCTAA GTCTTCTTTC TTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCTAA CAGCAACAAC ATTGAACCAG CACGAACTGC GGGAGTTAGT 
GGTCTTGCCA GGGCCGCCTC AAAGGACACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAAG AGCAGGCACA TAAATTTGTA GAGCGTTACT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCTACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CGTTTGAAAT CCAACACAGT GGATTTGTGA AGCAACTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAAGA CGCTGTGAGC AGAGAGATCA GATTAAAGAG ATTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG AACGAGTAGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAA ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTGCATGA CTTTCCCAGT GGAAATGGGA CAGGAGGCAG CTTCTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGT TCTGACGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGGGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGACG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCAT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACACGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTCCAGT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAT 
GAAGGTGTTT CCAGACAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGATTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTC 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCGAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001267.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857035.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

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CloneID	OBa227286
Clone ID Related Accession (Same CDS sequence)	XM_025001267.1
Accession Version	XM_025001267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCCTTA GCCAATACTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTCGCGGA TGCCGAACTT 
CTAAAGGATG TCCTGAAAAA CCATGCTGTT TCAAGTCATA TTGCTTCCAT GCTATCAAGC 
CAAGACCTGA AGATAGTAGT TGGAGCCCTT CAAATGGCAG AGATCTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCTG AGTCATTGTT GACAAGTCCA CCAAAGGCAT GTACAAATGG GTCCGGATCC 
TTGGGGTCCA CGCCATCGGT CAACAGTGGA ACAGCCACGG CCGCCACCAA TGCCTCGGCT 
GACCTGGGGT CCCCCAGCTT GCAGCACAGC AGAGACGATT CTCTAGACCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGATC AAGAAGGCCA 
AAGTATTCAC CTCCGAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACTACT 
ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA AGCTTGAATC CAAAAACGTG GGGAAGGTTA 
AGCGCACAGT CTAACAGCAA CAACATTGAA CCAGCACGAA CTGCGGGAGT TAGTGGTCTT 
GCCAGGGCCG CCTCAAAGGA CACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAAGAGCAGG CACATAAATT TGTAGAGCGT TACTTCAGTT CTGAGAACAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCTA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCTGATGTT 
TCATCGTTTG AAATCCAACA CAGTGGATTT GTGAAGCAAC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AAGACGCTGT GAGCAGAGAG ATCAGATTAA AGAGATTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGAACGAG TAGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAAATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTGC ATGACTTTCC CAGTGGAAAT GGGACAGGAG GCAGCTTCTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGCCAGTT ACAAAGACAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCCCTGGCT 
TTGGTGCAGG CTATTGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GACGAAGACA GTGACGACGA TGGTTCTGAC GAGGAAATAG ACGAATCTCT GGCTGCTCAG 
TTCTTAAATT CCGGAAATGT GAGACACAGG CTACAGTTTT ACATCGGGGA GCATTTGCTA 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTCA GTATACAGGC TGAAGATGAA 
CGGGAGTCCA CGGATGACGA GAGCAATCCT CTAGGAAGAG CTGGTATTTG GACGAAAACT 
CATACGATAT GGTACAAACC TGTGCGAGAG GACGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA GACAGCTCCA ACAAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGC TGTGGAATGA TGGAGTGTGC CCATCCGTAT CAAATCCTTT AGAAGTTTAC 
CTCATACCCA CAGCACCCGA AAATATAACT TTTGAAGACC CGTCATTAGA TGTAATTCTT 
CTTTTAAGAG TTTTACACGC CGTCAGTAGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAGA TTATCCCAAC TAGTGAATTT ATTAACAGTA AGTTGACAGC AAAAGCAAAT 
AGGCAGCTTC AAGATCCTTT GGTAATCATG ACAGGAAACA TCCCTACATG GCTCACTGAA 
CTAGGAAAAA CCTGCCCATT TTTCTTCCCT TTTGATACCC GGCAAATGCT TTTCTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAGATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ACAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTGCTGAA ACAAGCGGAG TCTGTGATGC AGGATCTTGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGCACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCAC TTGTATCTCA GGAACTGCAG AGAGCTGACT TGGGTCTCTG GAGAGGGGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAAGGCCTGT TTGCACTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCATAT CGCAAAGGTT 
AAAATGAAGT TTCGCTTCTT AGGAAAACTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTGGGCTT ACCTTTTTAT AAATGGATGC TCCGGCAAGA AACTTCGCTG 
ACGTCACACG ATTTGTTTGA CATTGACCCA GTTGTAGCCA GGTCAGTGTA TCACCTCGAA 
GACATCGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTC 
CAGTATGCAT TAGAAACCCT GACTATGAAT GGCTGCTCCG TGGAGGATCT GGGATTGGAT 
TTCACTCTGC CAGGGTTTCC TAACATTGAA CTAAAGAAAG GAGGAAAGGA TATACCGGTC 
ACCATCCACA ATTTGGAGGA GTATCTAAGA CTGGTTATAT TTTGGGCACT AAATGAAGGT 
GTTTCCAGAC AATTTGATTC ATTTAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTCCAGTACT TCTACCCAGA AGAACTGGAT CAGCTTCTGT GTGGTAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGC AGGCCAGATC ACGGTTACAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ACAATGAACA GCAGAGGTTA 
TTTCTCCAGT TTGTGACAGG TAGCCCACGA TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT 
CCACCTTTGA CCATTGTCCG GAAGACCTTC GAATCAACAG AGAACCCAGA TGACTTCCTA 
CCCTCTGTCA TGACCTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CCTCGAGATA 
ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857035.1
CDS	292..6411
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001267.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001267.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCCTTA GCCAATACTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTCGCGGA TGCCGAACTT 
CTAAAGGATG TCCTGAAAAA CCATGCTGTT TCAAGTCATA TTGCTTCCAT GCTATCAAGC 
CAAGACCTGA AGATAGTAGT TGGAGCCCTT CAAATGGCAG AGATCTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCTG AGTCATTGTT GACAAGTCCA CCAAAGGCAT GTACAAATGG GTCCGGATCC 
TTGGGGTCCA CGCCATCGGT CAACAGTGGA ACAGCCACGG CCGCCACCAA TGCCTCGGCT 
GACCTGGGGT CCCCCAGCTT GCAGCACAGC AGAGACGATT CTCTAGACCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGATC AAGAAGGCCA 
AAGTATTCAC CTCCGAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACTACT 
ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA AGCTTGAATC CAAAAACGTG GGGAAGGTTA 
AGCGCACAGT CTAACAGCAA CAACATTGAA CCAGCACGAA CTGCGGGAGT TAGTGGTCTT 
GCCAGGGCCG CCTCAAAGGA CACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAAGAGCAGG CACATAAATT TGTAGAGCGT TACTTCAGTT CTGAGAACAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCTA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCTGATGTT 
TCATCGTTTG AAATCCAACA CAGTGGATTT GTGAAGCAAC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AAGACGCTGT GAGCAGAGAG ATCAGATTAA AGAGATTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGAACGAG TAGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAAATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTGC ATGACTTTCC CAGTGGAAAT GGGACAGGAG GCAGCTTCTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGCCAGTT ACAAAGACAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCCCTGGCT 
TTGGTGCAGG CTATTGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GACGAAGACA GTGACGACGA TGGTTCTGAC GAGGAAATAG ACGAATCTCT GGCTGCTCAG 
TTCTTAAATT CCGGAAATGT GAGACACAGG CTACAGTTTT ACATCGGGGA GCATTTGCTA 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTCA GTATACAGGC TGAAGATGAA 
CGGGAGTCCA CGGATGACGA GAGCAATCCT CTAGGAAGAG CTGGTATTTG GACGAAAACT 
CATACGATAT GGTACAAACC TGTGCGAGAG GACGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA GACAGCTCCA ACAAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGC TGTGGAATGA TGGAGTGTGC CCATCCGTAT CAAATCCTTT AGAAGTTTAC 
CTCATACCCA CAGCACCCGA AAATATAACT TTTGAAGACC CGTCATTAGA TGTAATTCTT 
CTTTTAAGAG TTTTACACGC CGTCAGTAGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAGA TTATCCCAAC TAGTGAATTT ATTAACAGTA AGTTGACAGC AAAAGCAAAT 
AGGCAGCTTC AAGATCCTTT GGTAATCATG ACAGGAAACA TCCCTACATG GCTCACTGAA 
CTAGGAAAAA CCTGCCCATT TTTCTTCCCT TTTGATACCC GGCAAATGCT TTTCTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAGATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ACAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTGCTGAA ACAAGCGGAG TCTGTGATGC AGGATCTTGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGCACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCAC TTGTATCTCA GGAACTGCAG AGAGCTGACT TGGGTCTCTG GAGAGGGGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAAGGCCTGT TTGCACTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCATAT CGCAAAGGTT 
AAAATGAAGT TTCGCTTCTT AGGAAAACTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTGGGCTT ACCTTTTTAT AAATGGATGC TCCGGCAAGA AACTTCGCTG 
ACGTCACACG ATTTGTTTGA CATTGACCCA GTTGTAGCCA GGTCAGTGTA TCACCTCGAA 
GACATCGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTC 
CAGTATGCAT TAGAAACCCT GACTATGAAT GGCTGCTCCG TGGAGGATCT GGGATTGGAT 
TTCACTCTGC CAGGGTTTCC TAACATTGAA CTAAAGAAAG GAGGAAAGGA TATACCGGTC 
ACCATCCACA ATTTGGAGGA GTATCTAAGA CTGGTTATAT TTTGGGCACT AAATGAAGGT 
GTTTCCAGAC AATTTGATTC ATTTAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTCCAGTACT TCTACCCAGA AGAACTGGAT CAGCTTCTGT GTGGTAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGC AGGCCAGATC ACGGTTACAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ACAATGAACA GCAGAGGTTA 
TTTCTCCAGT TTGTGACAGG TAGCCCACGA TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT 
CCACCTTTGA CCATTGTCCG GAAGACCTTC GAATCAACAG AGAACCCAGA TGACTTCCTA 
CCCTCTGTCA TGACCTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CCTCGAGATA 
ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001269.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857037.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

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CloneID	OBa227287
Clone ID Related Accession (Same CDS sequence)	XM_025001269.1
Accession Version	XM_025001269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857037.1
CDS	186..6302
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001269.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001269.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG TTCTGACGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGGGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAC GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCA TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACACGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTCCAG 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA TGAAGGTGTT 
TCCAGACAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGATTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT CGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCGAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001273.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857041.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

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CloneID	OBa227288
Clone ID Related Accession (Same CDS sequence)	XM_025001273.1
Accession Version	XM_025001273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAGTTAAAA TGCCAGTTAC AAAGACATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CCCTGGCTTT GGTGCAGGCT 
ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA CGAAGACAGT 
GACGACGATG GTTCTGACGA GGAAATAGAC GAATCTCTGG CTGCTCAGTT CTTAAATTCC 
GGAAATGTGA GACACAGGCT ACAGTTTTAC ATCGGGGAGC ATTTGCTACC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTCAGT ATACAGGCTG AAGATGAACG GGAGTCCACG 
GATGACGAGA GCAATCCTCT AGGAAGAGCT GGTATTTGGA CGAAAACTCA TACGATATGG 
TACAAACCTG TGCGAGAGGA CGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAGA CAGCTCCAAC AAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGCTG 
TGGAATGATG GAGTGTGCCC ATCCGTATCA AATCCTTTAG AAGTTTACCT CATACCCACA 
GCACCCGAAA ATATAACTTT TGAAGACCCG TCATTAGATG TAATTCTTCT TTTAAGAGTT 
TTACACGCCG TCAGTAGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAGATT 
ATCCCAACTA GTGAATTTAT TAACAGTAAG TTGACAGCAA AAGCAAATAG GCAGCTTCAA 
GATCCTTTGG TAATCATGAC AGGAAACATC CCTACATGGC TCACTGAACT AGGAAAAACC 
TGCCCATTTT TCTTCCCTTT TGATACCCGG CAAATGCTTT TCTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AGATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAC AGAAAAAAAC GTACTGTGAA CAGAGAGGAG 
CTGCTGAAAC AAGCGGAGTC TGTGATGCAG GATCTTGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGCACAGGT CTTGGGCCTA CACTGGAGTT TTATGCACTT 
GTATCTCAGG AACTGCAGAG AGCTGACTTG GGTCTCTGGA GAGGGGAAGA AGTAACTCTT 
AGCAATCCAA AAGGAAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA AGGCCTGTTT 
GCACTTCCCT TTGGTAGGAC AGCTAAGCCA GCTCATATCG CAAAGGTTAA AATGAAGTTT 
CGCTTCTTAG GAAAACTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTGGGCTTAC CTTTTTATAA ATGGATGCTC CGGCAAGAAA CTTCGCTGAC GTCACACGAT 
TTGTTTGACA TTGACCCAGT TGTAGCCAGG TCAGTGTATC ACCTCGAAGA CATCGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTCCA GTATGCATTA 
GAAACCCTGA CTATGAATGG CTGCTCCGTG GAGGATCTGG GATTGGATTT CACTCTGCCA 
GGGTTTCCTA ACATTGAACT AAAGAAAGGA GGAAAGGATA TACCGGTCAC CATCCACAAT 
TTGGAGGAGT ATCTAAGACT GGTTATATTT TGGGCACTAA ATGAAGGTGT TTCCAGACAA 
TTTGATTCAT TTAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT CCAGTACTTC 
TACCCAGAAG AACTGGATCA GCTTCTGTGT GGTAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGCAG GCCAGATCAC GGTTACACTC ATGACAGTCG GGCTGTGAAG 
TTCTTGTTTG AGATTCTCAG TAGTTTTGAC AATGAACAGC AGAGGTTATT TCTCCAGTTT 
GTGACAGGTA GCCCACGATT GCCTGTTGGA GGCTTCCGGA GCTTGAATCC ACCTTTGACC 
ATTGTCCGGA AGACCTTCGA ATCAACAGAG AACCCAGATG ACTTCCTACC CTCTGTCATG 
ACCTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCC TCGAGATAAT GCGGGAAAAG 
CTGTTGATGG CTGCGAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857041.1
CDS	292..6399
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001273.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001273.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAGTTAAAA TGCCAGTTAC AAAGACATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CCCTGGCTTT GGTGCAGGCT 
ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA CGAAGACAGT 
GACGACGATG GTTCTGACGA GGAAATAGAC GAATCTCTGG CTGCTCAGTT CTTAAATTCC 
GGAAATGTGA GACACAGGCT ACAGTTTTAC ATCGGGGAGC ATTTGCTACC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTCAGT ATACAGGCTG AAGATGAACG GGAGTCCACG 
GATGACGAGA GCAATCCTCT AGGAAGAGCT GGTATTTGGA CGAAAACTCA TACGATATGG 
TACAAACCTG TGCGAGAGGA CGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAGA CAGCTCCAAC AAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGCTG 
TGGAATGATG GAGTGTGCCC ATCCGTATCA AATCCTTTAG AAGTTTACCT CATACCCACA 
GCACCCGAAA ATATAACTTT TGAAGACCCG TCATTAGATG TAATTCTTCT TTTAAGAGTT 
TTACACGCCG TCAGTAGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAGATT 
ATCCCAACTA GTGAATTTAT TAACAGTAAG TTGACAGCAA AAGCAAATAG GCAGCTTCAA 
GATCCTTTGG TAATCATGAC AGGAAACATC CCTACATGGC TCACTGAACT AGGAAAAACC 
TGCCCATTTT TCTTCCCTTT TGATACCCGG CAAATGCTTT TCTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AGATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAC AGAAAAAAAC GTACTGTGAA CAGAGAGGAG 
CTGCTGAAAC AAGCGGAGTC TGTGATGCAG GATCTTGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGCACAGGT CTTGGGCCTA CACTGGAGTT TTATGCACTT 
GTATCTCAGG AACTGCAGAG AGCTGACTTG GGTCTCTGGA GAGGGGAAGA AGTAACTCTT 
AGCAATCCAA AAGGAAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA AGGCCTGTTT 
GCACTTCCCT TTGGTAGGAC AGCTAAGCCA GCTCATATCG CAAAGGTTAA AATGAAGTTT 
CGCTTCTTAG GAAAACTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTGGGCTTAC CTTTTTATAA ATGGATGCTC CGGCAAGAAA CTTCGCTGAC GTCACACGAT 
TTGTTTGACA TTGACCCAGT TGTAGCCAGG TCAGTGTATC ACCTCGAAGA CATCGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTCCA GTATGCATTA 
GAAACCCTGA CTATGAATGG CTGCTCCGTG GAGGATCTGG GATTGGATTT CACTCTGCCA 
GGGTTTCCTA ACATTGAACT AAAGAAAGGA GGAAAGGATA TACCGGTCAC CATCCACAAT 
TTGGAGGAGT ATCTAAGACT GGTTATATTT TGGGCACTAA ATGAAGGTGT TTCCAGACAA 
TTTGATTCAT TTAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT CCAGTACTTC 
TACCCAGAAG AACTGGATCA GCTTCTGTGT GGTAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGCAG GCCAGATCAC GGTTACACTC ATGACAGTCG GGCTGTGAAG 
TTCTTGTTTG AGATTCTCAG TAGTTTTGAC AATGAACAGC AGAGGTTATT TCTCCAGTTT 
GTGACAGGTA GCCCACGATT GCCTGTTGGA GGCTTCCGGA GCTTGAATCC ACCTTTGACC 
ATTGTCCGGA AGACCTTCGA ATCAACAGAG AACCCAGATG ACTTCCTACC CTCTGTCATG 
ACCTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCC TCGAGATAAT GCGGGAAAAG 
CTGTTGATGG CTGCGAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001283.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857051.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

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CloneID	OBa227289
Clone ID Related Accession (Same CDS sequence)	XM_025001283.1
Accession Version	XM_025001283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGTTCTG ACGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGG GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGACGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCATCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA CGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TCCAGTATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAATGAAG GTGTTTCCAG ACAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GATTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTCGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857051.1
CDS	292..6393
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001283.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001283.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAAGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT ATCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC AAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACGC CATCGGTCAA CAGTGGAACA GCCACGGCCG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACTACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCTACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAACTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAAG ACGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGTTCTG ACGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGG GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGACGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCATCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA CGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TCCAGTATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAATGAAG GTGTTTCCAG ACAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GATTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTCGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001302.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857070.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X26, mRNA.

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CloneID	OBa227290
Clone ID Related Accession (Same CDS sequence)	XM_025001302.1
Accession Version	XM_025001302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTCGCGG ATGCCGAACT TCTAAAGGAT GTCCTGAAAA ACCATGCTGT TTCAAGTCAT 
ATTGCTTCCA TGCTATCAAG CCAAGACCTG AAGATAGTAG TTGGAGCCCT TCAAATGGCA 
GAGATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCT GAGTCATTGT TGACAAGTCC ACCAAAGGCA 
TGTACAAATG GGTCCGGATC CTTGGGGTCC ACGCCATCGG TCAACAGTGG AACAGCCACG 
GCCGCCACCA ATGCCTCGGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGACGAT 
TCTCTAGACC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGAT CAAGAAGGCC AAAGTATTCA CCTCCGAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACTAC TACTCAGTCA CCTAAGTCTT CTTTCTTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCTAACAGCA ACAACATTGA ACCAGCACGA 
ACTGCGGGAG TTAGTGGTCT TGCCAGGGCC GCCTCAAAGG ACACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAAGAGCAG GCACATAAAT TTGTAGAGCG TTACTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCT 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCGTTT GAAATCCAAC ACAGTGGATT TGTGAAGCAA 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAAGACGCTG TGAGCAGAGA GATCAGATTA 
AAGAGATTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGAACGA 
GTAGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAAATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTG CATGACTTTC CCAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGT TCTGACGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGGGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGACG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCAT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACACGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTCCAGT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAT 
GAAGGTGTTT CCAGACAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGATTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTC 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCGAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857070.1
CDS	291..6356
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001302.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTCGCGG ATGCCGAACT TCTAAAGGAT GTCCTGAAAA ACCATGCTGT TTCAAGTCAT 
ATTGCTTCCA TGCTATCAAG CCAAGACCTG AAGATAGTAG TTGGAGCCCT TCAAATGGCA 
GAGATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCT GAGTCATTGT TGACAAGTCC ACCAAAGGCA 
TGTACAAATG GGTCCGGATC CTTGGGGTCC ACGCCATCGG TCAACAGTGG AACAGCCACG 
GCCGCCACCA ATGCCTCGGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGACGAT 
TCTCTAGACC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGAT CAAGAAGGCC AAAGTATTCA CCTCCGAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACTAC TACTCAGTCA CCTAAGTCTT CTTTCTTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCTAACAGCA ACAACATTGA ACCAGCACGA 
ACTGCGGGAG TTAGTGGTCT TGCCAGGGCC GCCTCAAAGG ACACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAAGAGCAG GCACATAAAT TTGTAGAGCG TTACTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCT 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCGTTT GAAATCCAAC ACAGTGGATT TGTGAAGCAA 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAAGACGCTG TGAGCAGAGA GATCAGATTA 
AAGAGATTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGAACGA 
GTAGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAAATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTG CATGACTTTC CCAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGT TCTGACGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGGGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGACG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCAT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACACGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTCCAGT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAT 
GAAGGTGTTT CCAGACAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGATTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTC 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCGAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001310.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857078.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X27, mRNA.

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CloneID	OBa227291
Clone ID Related Accession (Same CDS sequence)	XM_025001310.1
Accession Version	XM_025001310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857078.1
CDS	339..6401
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001310.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001310.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001322.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857090.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X28, mRNA.

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CloneID	OBa227292
Clone ID Related Accession (Same CDS sequence)	XM_025001322.1
Accession Version	XM_025001322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA AACATCCAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC CAAGAAGCGC 
AGCGAATCCC CGCCTGCCGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGCG CTAGTGCCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAGAT GGCCGACCCT GAAGGCAACC AGGAGACGGT GAACTCTTCA 
GCTGCACGGA CAGACGAGAC TCCCCAAGGA GCAGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTGGGCATGA CCACCTCTGG GGAGAGCGAA TCTGATGACT CCGAGATGGG ACGGTTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCCCCT CACCTGTTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTG 
CAAGGACTAC AAGCAAGCGA CGAAAGCCAG CAGCTCCAAG CCGTTATTGA GATGTGTCAG 
TTACTGGTTA TGGGGAATGA GGAGACGCTG GGAGGGTTTC CTGTCAAGAG TGTAGTTCCA 
GCTTTGATAA CATTGCTGCA GATGGAACAT AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCATCGG CTGTGGTAGT TGATGCAATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCCGA GCAGGCCTTG 
ACGGCCTTGG AGATGTTATC ACGTAGACAC AGTAAAGCCA TTCTCCAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTATCT AGAGTTCTTC AGCATAAATG CCCAAAGAAA CGCGCTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCGGATTCC 
CTCCCATTGC TTACCCAAAG GCTAACCCAC CAGGACAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGTCTAGT GGACAACTTC CAACATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTAAC AAATGTTCAA CAACTGTTGG TAGTAACTCC ACCAATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAATTGTCCA 
ACTTTAGCAG TTCAACTTAT GAAGCAAAGC TTTACAGACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAAGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGGTAGCAGC CCATCAGGTC GGTGAGGATG AGATAAGCTT GTCCACTCTG 
GGACGAGTTT ATACTATTGA TTTTAATTCT ATGCAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857090.1
CDS	165..6167
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001322.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001322.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA AACATCCAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC CAAGAAGCGC 
AGCGAATCCC CGCCTGCCGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGCG CTAGTGCCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAGAT GGCCGACCCT GAAGGCAACC AGGAGACGGT GAACTCTTCA 
GCTGCACGGA CAGACGAGAC TCCCCAAGGA GCAGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTGGGCATGA CCACCTCTGG GGAGAGCGAA TCTGATGACT CCGAGATGGG ACGGTTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCCCCT CACCTGTTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTG 
CAAGGACTAC AAGCAAGCGA CGAAAGCCAG CAGCTCCAAG CCGTTATTGA GATGTGTCAG 
TTACTGGTTA TGGGGAATGA GGAGACGCTG GGAGGGTTTC CTGTCAAGAG TGTAGTTCCA 
GCTTTGATAA CATTGCTGCA GATGGAACAT AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCATCGG CTGTGGTAGT TGATGCAATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCCGA GCAGGCCTTG 
ACGGCCTTGG AGATGTTATC ACGTAGACAC AGTAAAGCCA TTCTCCAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTATCT AGAGTTCTTC AGCATAAATG CCCAAAGAAA CGCGCTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCGGATTCC 
CTCCCATTGC TTACCCAAAG GCTAACCCAC CAGGACAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGTCTAGT GGACAACTTC CAACATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTAAC AAATGTTCAA CAACTGTTGG TAGTAACTCC ACCAATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAATTGTCCA 
ACTTTAGCAG TTCAACTTAT GAAGCAAAGC TTTACAGACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAAGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGGTAGCAGC CCATCAGGTC GGTGAGGATG AGATAAGCTT GTCCACTCTG 
GGACGAGTTT ATACTATTGA TTTTAATTCT ATGCAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001214.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856982.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OBa16781
Clone ID Related Accession (Same CDS sequence)	XM_025001214.1 , XM_015459467.1
Accession Version	XM_025001214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG TTCTGACGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGGGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAC 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCA 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACACGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTCCAG TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA TGAAGGTGTT TCCAGACAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGATTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT CGAGATAATG CGGGAAAAGC TGTTGATGGC TGCGAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856982.1
CDS	291..6497
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001214.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001214.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG TTCTGACGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGGGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAC 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCA 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACACGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTCCAG TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA TGAAGGTGTT TCCAGACAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGATTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT CGAGATAATG CGGGAAAAGC TGTTGATGGC TGCGAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459467.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314953.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OBa16781
Clone ID Related Accession (Same CDS sequence)	XM_025001214.1 , XM_015459467.1
Accession Version	XM_015459467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG TTCTGACGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGGGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAC 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCA 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACACGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTCCAG TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA TGAAGGTGTT TCCAGACAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGATTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT CGAGATAATG CGGGAAAAGC TGTTGATGGC TGCGAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314953.1
CDS	179..6385
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459467.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459467.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT ATCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC AAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACGC CATCGGTCAA CAGTGGAACA 
GCCACGGCCG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACTACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCTACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAACTGT TGCTTTATTT GACATCTAAA AGTGAAAAAG ACGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG TTCTGACGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGGGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAC 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCA 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACACGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTCCAG TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA TGAAGGTGTT TCCAGACAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGATTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT CGAGATAATG CGGGAAAAGC TGTTGATGGC TGCGAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001222.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856990.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OBa16782
Clone ID Related Accession (Same CDS sequence)	XM_025001222.1 , XM_015459470.1
Accession Version	XM_025001222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGTTC TGACGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GGGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGACGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCATCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CACGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTCCAGTAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAATGA AGGTGTTTCC AGACAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGATTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTCGA GATAATGCGG GAAAAGCTGT TGATGGCTGC GAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856990.1
CDS	256..6459
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001222.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001222.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGTTC TGACGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GGGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGACGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCATCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CACGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTCCAGTAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAATGA AGGTGTTTCC AGACAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGATTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTCGA GATAATGCGG GAAAAGCTGT TGATGGCTGC GAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459470.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314956.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OBa16782
Clone ID Related Accession (Same CDS sequence)	XM_025001222.1 , XM_015459470.1
Accession Version	XM_015459470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGTTC TGACGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GGGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGACGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCATCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CACGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTCCAGTAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAATGA AGGTGTTTCC AGACAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGATTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTCGA GATAATGCGG GAAAAGCTGT TGATGGCTGC GAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314956.1
CDS	179..6382
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459470.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459470.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTATC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACAAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACGCCAT CGGTCAACAG TGGAACAGCC 
ACGGCCGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC TACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCTACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAACTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAAGACG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGTTC TGACGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GGGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGACGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCATCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CACGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTCCAGTAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAATGA AGGTGTTTCC AGACAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGATTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTCGA GATAATGCGG GAAAAGCTGT TGATGGCTGC GAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459479.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314965.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OBa16785
Clone ID Related Accession (Same CDS sequence)	XM_015459479.1 , XM_025001263.1
Accession Version	XM_015459479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314965.1
CDS	179..6301
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459479.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459479.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001263.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857031.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

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CloneID	OBa16785
Clone ID Related Accession (Same CDS sequence)	XM_015459479.1 , XM_025001263.1
Accession Version	XM_025001263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857031.1
CDS	291..6413
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001263.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001263.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAA 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTATCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACAAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACGCCATC GGTCAACAGT GGAACAGCCA CGGCCGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACT 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CTACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AACTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAAGACGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGTTCT GACGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG GGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGACGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCATCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ACGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTCCAGTATG CATTAGAAAC CCTGACTATG AATGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAATGAA 
GGTGTTTCCA GACAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGATTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTCGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCG AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001290.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857058.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

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CloneID	OBa16787
Clone ID Related Accession (Same CDS sequence)	XM_025001290.1 , XM_015459483.1 , XM_015459483.2
Accession Version	XM_025001290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857058.1
CDS	320..6397
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001290.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001290.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314969.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OBa16787
Clone ID Related Accession (Same CDS sequence)	XM_025001290.1 , XM_015459483.1 , XM_015459483.2
Accession Version	XM_015459483.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314969.1
CDS	178..6255
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459483.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459483.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314969.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

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CloneID	OBa16787
Clone ID Related Accession (Same CDS sequence)	XM_025001290.1 , XM_015459483.1 , XM_015459483.2
Accession Version	XM_015459483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314969.1
CDS	180..6257
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459483.2
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459483.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAAGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TATCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CAAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACG CCATCGGTCA ACAGTGGAAC AGCCACGGCC 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACTACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCTACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAACTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAA GACGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GTTCTGACGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGGGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA CGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC ATCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACACGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTCCA GTATGCATTA GAAACCCTGA CTATGAATGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ATGAAGGTGT TTCCAGACAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGATT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TCGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCGAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_010802409.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800711.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_010802409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800711.1
CDS	190..6405
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_010802409.3
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802409.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_010802410.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800712.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_010802410.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800712.1
CDS	181..6396
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_010802410.3
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802410.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001202.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856970.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_025001202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856970.1
CDS	74..6289
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001202.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001202.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001205.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856973.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_025001205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856973.1
CDS	73..6288
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001205.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001205.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001187.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856955.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_025001187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856955.1
CDS	292..6507
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001187.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001187.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001197.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856965.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_025001197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856965.1
CDS	72..6287
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001197.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001197.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_010802410.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800712.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_010802410.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800712.1
CDS	180..6395
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_010802410.2
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802410.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_010802409.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800711.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_010802409.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800711.1
CDS	1719..7934
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_010802409.2
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802409.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001195.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856963.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OBa48935
Clone ID Related Accession (Same CDS sequence)	XM_010802409.3 , XM_010802410.3 , XM_025001202.1 , XM_025001205.1 , XM_025001187.1 , XM_025001197.1 , XM_010802410.2 , XM_010802409.2 , XM_025001195.1
Accession Version	XM_025001195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856963.1
CDS	166..6381
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001195.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001195.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGT 
TCTGACGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGGGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGACG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCAT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC ACTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACACGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTCCAGT ATGCATTAGA AACCCTGACT 
ATGAATGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAT GAAGGTGTTT CCAGACAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGATTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTC GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCGAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459460.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314946.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OBa48936
Clone ID Related Accession (Same CDS sequence)	XM_015459460.1 , XM_025001208.1
Accession Version	XM_015459460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTATCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACAAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACGCCATC GGTCAACAGT 
GGAACAGCCA CGGCCGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACT ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCTG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CTACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AACTGTTGCT TTATTTGACA TCTAAAAGTG AAAAAGACGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATACCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGTTCT 
GACGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG GGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGACGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCATCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCACT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ACGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTCCAGTATG CATTAGAAAC CCTGACTATG 
AATGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAATGAA GGTGTTTCCA GACAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGATTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTCGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCG 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314946.1
CDS	180..6392
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459460.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459460.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTATCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACAAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACGCCATC GGTCAACAGT 
GGAACAGCCA CGGCCGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACT ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCTG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CTACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AACTGTTGCT TTATTTGACA TCTAAAAGTG AAAAAGACGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATACCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGTTCT 
GACGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG GGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGACGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCATCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCACT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ACGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTCCAGTATG CATTAGAAAC CCTGACTATG 
AATGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAATGAA GGTGTTTCCA GACAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGATTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTCGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCG 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001208.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856976.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OBa48936
Clone ID Related Accession (Same CDS sequence)	XM_015459460.1 , XM_025001208.1
Accession Version	XM_025001208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTATCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACAAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACGCCATC GGTCAACAGT 
GGAACAGCCA CGGCCGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACT ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCTG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CTACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AACTGTTGCT TTATTTGACA TCTAAAAGTG AAAAAGACGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATACCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGTTCT 
GACGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG GGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGACGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCATCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCACT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ACGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTCCAGTATG CATTAGAAAC CCTGACTATG 
AATGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAATGAA GGTGTTTCCA GACAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGATTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTCGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCG 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856976.1
CDS	291..6503
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001208.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001208.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTATCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACAAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACGCCATC GGTCAACAGT 
GGAACAGCCA CGGCCGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACT ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCTG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CTACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AACTGTTGCT TTATTTGACA TCTAAAAGTG AAAAAGACGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATACCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGTTCT 
GACGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG GGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGACGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCATCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCACT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ACGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTCCAGTATG CATTAGAAAC CCTGACTATG 
AATGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAATGAA GGTGTTTCCA GACAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGATTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTCGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCG 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459471.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314957.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OBa48937
Clone ID Related Accession (Same CDS sequence)	XM_015459471.1 , XM_025001231.1
Accession Version	XM_015459471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGTTCTGA CGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGGG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGACGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCATCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAC GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT CCAGTATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAATGAAGG TGTTTCCAGA CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG ATTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTCGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314957.1
CDS	181..6381
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459471.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459471.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGTTCTGA CGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGGG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGACGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCATCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAC GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT CCAGTATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAATGAAGG TGTTTCCAGA CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG ATTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTCGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001231.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856999.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OBa48937
Clone ID Related Accession (Same CDS sequence)	XM_015459471.1 , XM_025001231.1
Accession Version	XM_025001231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGTTCTGA CGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGGG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGACGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCATCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAC GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT CCAGTATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAATGAAGG TGTTTCCAGA CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG ATTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTCGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856999.1
CDS	293..6493
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001231.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001231.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTA TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACA 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACGCC ATCGGTCAAC 
AGTGGAACAG CCACGGCCGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACTACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCTACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAACTGTT GCTTTATTTG ACATCTAAAA GTGAAAAAGA CGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGTTCTGA CGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGGG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGACGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCATCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAC GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT CCAGTATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAATGAAGG TGTTTCCAGA CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG ATTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTCGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCGAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001240.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857008.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

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CloneID	OBa48938
Clone ID Related Accession (Same CDS sequence)	XM_025001240.1 , XM_015459474.1
Accession Version	XM_025001240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTAT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACAA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACGCCA TCGGTCAACA GTGGAACAGC CACGGCCGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CTACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC TGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCTACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAACTGTTG 
CTTTATTTGA CATCTAAAAG TGAAAAAGAC GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATACC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGTT CTGACGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGGGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGACGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCATC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCA CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACACGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTCCAGTA TGCATTAGAA ACCCTGACTA TGAATGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAATG AAGGTGTTTC CAGACAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGATTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTCG AGATAATGCG GGAAAAGCTG TTGATGGCTG CGAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857008.1
CDS	292..6426
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001240.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001240.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTAT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACAA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACGCCA TCGGTCAACA GTGGAACAGC CACGGCCGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CTACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC TGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCTACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAACTGTTG 
CTTTATTTGA CATCTAAAAG TGAAAAAGAC GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATACC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGTT CTGACGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGGGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGACGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCATC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCA CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACACGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTCCAGTA TGCATTAGAA ACCCTGACTA TGAATGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAATG AAGGTGTTTC CAGACAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGATTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTCG AGATAATGCG GGAAAAGCTG TTGATGGCTG CGAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459474.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314960.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OBa48938
Clone ID Related Accession (Same CDS sequence)	XM_025001240.1 , XM_015459474.1
Accession Version	XM_015459474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTAT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACAA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACGCCA TCGGTCAACA GTGGAACAGC CACGGCCGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CTACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC TGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCTACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAACTGTTG 
CTTTATTTGA CATCTAAAAG TGAAAAAGAC GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATACC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGTT CTGACGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGGGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGACGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCATC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCA CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACACGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTCCAGTA TGCATTAGAA ACCCTGACTA TGAATGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAATG AAGGTGTTTC CAGACAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGATTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTCG AGATAATGCG GGAAAAGCTG TTGATGGCTG CGAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314960.1
CDS	180..6314
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459474.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459474.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTAT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACAA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACGCCA TCGGTCAACA GTGGAACAGC CACGGCCGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CTACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC TGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCTACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAACTGTTG 
CTTTATTTGA CATCTAAAAG TGAAAAAGAC GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATACC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGTT CTGACGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGGGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGACGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCATC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCA CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACACGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTCCAGTA TGCATTAGAA ACCCTGACTA TGAATGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAATG AAGGTGTTTC CAGACAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGATTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTCG AGATAATGCG GGAAAAGCTG TTGATGGCTG CGAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459475.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314961.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OBa48939
Clone ID Related Accession (Same CDS sequence)	XM_015459475.1 , XM_025001243.1
Accession Version	XM_015459475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTATCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACAAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACGCCATCG GTCAACAGTG GAACAGCCAC GGCCGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACTA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCTGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC TACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA ACTGTTGCTT 
TATTTGACAT CTAAAAGTGA AAAAGACGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314961.1
CDS	180..6311
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459475.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459475.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTATCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACAAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACGCCATCG GTCAACAGTG GAACAGCCAC GGCCGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACTA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCTGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC TACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA ACTGTTGCTT 
TATTTGACAT CTAAAAGTGA AAAAGACGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001243.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857011.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

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CloneID	OBa48939
Clone ID Related Accession (Same CDS sequence)	XM_015459475.1 , XM_025001243.1
Accession Version	XM_025001243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTATCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACAAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACGCCATCG GTCAACAGTG GAACAGCCAC GGCCGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACTA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCTGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC TACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA ACTGTTGCTT 
TATTTGACAT CTAAAAGTGA AAAAGACGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857011.1
CDS	292..6423
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001243.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001243.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAAACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGACGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGACGAAAGC 
CAGCAGCTCC AAGCCGTTAT TGAGATGTGT CAGTTACTGG TTATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTAGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CGGCTGTGGT AGTTGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAGCAGGCC TTGACGGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCGCTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAAG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTATCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACAAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACGCCATCG GTCAACAGTG GAACAGCCAC GGCCGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACTA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCTGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC TACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA ACTGTTGCTT 
TATTTGACAT CTAAAAGTGA AAAAGACGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGTTCTG ACGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGG 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGACGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCATCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA CGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TCCAGTATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAATGAAG GTGTTTCCAG ACAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GATTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTCGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCGA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459476.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314962.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OBa48940
Clone ID Related Accession (Same CDS sequence)	XM_015459476.1 , XM_025001250.1
Accession Version	XM_015459476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AAGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTATCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACAAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC GCCATCGGTC AACAGTGGAA CAGCCACGGC CGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACTACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCTAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAACT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA AGACGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314962.1
CDS	180..6308
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459476.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459476.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AAGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTATCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACAAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC GCCATCGGTC AACAGTGGAA CAGCCACGGC CGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACTACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCTAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAACT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA AGACGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001250.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857018.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

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CloneID	OBa48940
Clone ID Related Accession (Same CDS sequence)	XM_015459476.1 , XM_025001250.1
Accession Version	XM_025001250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AAGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTATCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACAAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC GCCATCGGTC AACAGTGGAA CAGCCACGGC CGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACTACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCTAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAACT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA AGACGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857018.1
CDS	292..6420
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001250.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001250.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CGGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCGTCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCGCG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCATCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAAACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA CGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGACGAA AGCCAGCAGC TCCAAGCCGT TATTGAGATG 
TGTCAGTTAC TGGTTATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTA 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCGGCTGT GGTAGTTGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAGCAG 
GCCTTGACGG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCG 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AAGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTATCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACAAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC GCCATCGGTC AACAGTGGAA CAGCCACGGC CGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACTACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCTAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAACT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA AGACGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGTTCTGACG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGGGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ACGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CATCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACACGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTCC AGTATGCATT AGAAACCCTG ACTATGAATG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AATGAAGGTG TTTCCAGACA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGAT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTCGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCGAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025001288.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857056.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

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CloneID	OBa48941
Clone ID Related Accession (Same CDS sequence)	XM_025001288.1 , XM_015459481.1
Accession Version	XM_025001288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAAGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTATCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACAAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CGCCATCGGT CAACAGTGGA 
ACAGCCACGG CCGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACTACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCTGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCTA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAAC TGTTGCTTTA TTTGACATCT AAAAGTGAAA AAGACGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATACCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGTTCTGAC 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGGGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GACGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCATCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCACTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACACG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTC CAGTATGCAT TAGAAACCCT GACTATGAAT 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAATGAAGGT GTTTCCAGAC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGA 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTCGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857056.1
CDS	291..6380
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025001288.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001288.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAAGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTATCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACAAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CGCCATCGGT CAACAGTGGA 
ACAGCCACGG CCGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACTACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCTGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCTA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAAC TGTTGCTTTA TTTGACATCT AAAAGTGAAA AAGACGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATACCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGTTCTGAC 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGGGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GACGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCATCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCACTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACACG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTC CAGTATGCAT TAGAAACCCT GACTATGAAT 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAATGAAGGT GTTTCCAGAC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGA 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTCGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015459481.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314967.1 Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OBa48941
Clone ID Related Accession (Same CDS sequence)	XM_025001288.1 , XM_015459481.1
Accession Version	XM_015459481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAAGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTATCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACAAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CGCCATCGGT CAACAGTGGA 
ACAGCCACGG CCGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACTACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCTGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCTA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAAC TGTTGCTTTA TTTGACATCT AAAAGTGAAA AAGACGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATACCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGTTCTGAC 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGGGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GACGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCATCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCACTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACACG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTC CAGTATGCAT TAGAAACCCT GACTATGAAT 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAATGAAGGT GTTTCCAGAC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGA 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTCGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314967.1
CDS	179..6268
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015459481.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459481.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCGGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCGTC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CGCGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC ATCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAAAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ACGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGACGA AAGCCAGCAG 
CTCCAAGCCG TTATTGAGAT GTGTCAGTTA CTGGTTATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT AGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCGGCTG TGGTAGTTGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAGCA GGCCTTGACG GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC GCTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAAGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTATCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACAAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CGCCATCGGT CAACAGTGGA 
ACAGCCACGG CCGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACTACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCTGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCTA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAAC TGTTGCTTTA TTTGACATCT AAAAGTGAAA AAGACGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATACCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGTTCTGAC 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGGGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GACGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCATCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCACTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACACG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTC CAGTATGCAT TAGAAACCCT GACTATGAAT 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAATGAAGGT GTTTCCAGAC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGA 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTCGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCGAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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オンライン注文

TRIP12 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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