EHMT2 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EHMT2
Entrez Gene ID	514062
Full Name	euchromatic histone lysine methyltransferase 2
Synonyms	BAT8
General protein information	Preferred Names euchromatic histone lysine methyltransferase 2 Names histone-lysine N-methyltransferase EHMT2 ankyrin repeat-containing protein euchromatic histone-lysine N-methyltransferase 2 histone-lysine N-methyltransferase, H3 lysine-9 specific 3
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005223636.2	XP_005223693.1	histone-lysine N-methyltransferase EHMT2 isoform X1
XM_024983572.1	XP_024839340.1	histone-lysine N-methyltransferase EHMT2 isoform X1
XM_024983573.1	XP_024839341.1	histone-lysine N-methyltransferase EHMT2 isoform X2
XM_005223636.3	XP_005223693.1	histone-lysine N-methyltransferase EHMT2 isoform X3
NM_001206263.2	NP_001193192.2	histone-lysine N-methyltransferase EHMT2
NM_001206263.1	NP_001193192.1	histone-lysine N-methyltransferase EHMT2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EHMT2	XP_518365.3
Canis lupus familiaris (dog)	EHMT2	XP_005627223.1
Rattus norvegicus (Norway rat)	Ehmt2	NP_997628.1
Danio rerio (zebrafish)	ehmt2	NP_001107087.1
Bos taurus (cattle)	EHMT2	NP_001193192.1
Mus musculus (house mouse)	Ehmt2	NP_665829.1
Homo sapiens (human)	EHMT2	NP_006700.3
	EHMT2	XP_001106224.2
Xenopus tropicalis (tropical clawed frog)	ehmt2	XP_004917185.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of EHMT2 Induces a Robust Antiviral Response Against Foot-and-Mouth Disease and Vesicular Stomatitis Virus Infections in Bovine Cells.
Singh N, Ram?rez-Carvajal L, de Los Santos T, Golding MC, Long CR
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research36(1)37-47(2016 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

YAC/BAC contig spanning the MHC class III region of cattle.
Gelhaus A, Hess M, F?rster B, Goldammer T, Schwerin M, Horstmann RD
Cytogenetic and genome research115(1)45-50(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data confirm the involvement of EHMT2 in the epigenetic regulation of IFN-b and demonstrate the activation of a general antiviral state after EHMT2 inhibition.
Title: Inhibition of EHMT2 Induces a Robust Antiviral Response Against Foot-and-Mouth Disease and Vesicular Stomatitis Virus Infections in Bovine Cells.

The following EHMT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHMT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229477	XM_024983572.1 Latest version!	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229478	XM_024983573.1 Latest version!	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa216189	NM_001206263.2 Latest version!	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa41249	NM_001206263.1 Latest version!	Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa32136	XM_005223636.3 Latest version!	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OBa32136	XM_005223636.2	Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EHMT2 ( XM_024983572.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024839340.1 Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229477
Clone ID Related Accession (Same CDS sequence)	XM_024983572.1
Accession Version	XM_024983572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase EHMT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGTTG ATGCGGGCCC GGGGGAGGGG TCGTGCGGCC 
CCTCCGGGCA GCCGAGGCCG CGGAAGGGGG GGAGCCCATA GAGGAAGAGG TAGGCCCCGG 
AGCCTGCTCT CTCTACCCAG GGCCCAGGCG TCCTGGGCCC CCCAGCTTCC TACCGGACTG 
ACCAGCCCTC CTATCCCTTG TGTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGGTGCTGG AGAAGGAGCC CAGAGGAGCC ACCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCGTA GTGAGGAGAC CCTGCCCAAG GCCAACCCCG ATTCCCTGGA GACTGCTGGC 
CCCTCATCCC CAGCCTCTGT CACAGTCACT GTCGGCGATG AGGGGGCTGA CACCCCTGTA 
GGGGCCACAC CACTCATTGG GGATGAACCC GAGAATCTCG AGGGAGATGG GGACCTCCAT 
GGGGGTCGCA TCCTGCTGGG CCATGCCACA AAGTCGTTCC CGTCTTCCCC CAGCAAGGGG 
GGTGCCTGTC CCAGCCGAGC CAAGATGTCA ATGACAGGGG CCGGAAAATC ACCCCCATCA 
GTTCAGAGTT TGGCTATGAG GCTGCTAAGT ATGCCGGGCG CCCAAGGGGC GCCAGCAGCA 
GGGCCTGAAC CCCCTCCGGC CACAGCCAGC CCAGAGGGGC AGCCCAAGGT TCATCGAGCC 
AGGAAAACCA TGTCCAAACC AGGAAATGGA CAGCCCCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTGCAGC ATTTCCGCAT GAGTGATGAC GTGCACTCGC TGGGGAAGGT GACCTCAGAT 
GTGGCCAAAA GGAGGAAGCT GAACTCAGGA GGTGGCTTGT CAGAAGAGTT GGCTTCTACG 
CGGAGTTCGG GAGAAGTGAC CCTGGAGAAG GGGGACCCCG GGTCCCTGGA GGAGTGGGAA 
ACGGTGGTAG GGGATGACTT CAGCCTCTAC TATGATTCCT ACTCTGTGGA TGAGCGGGTG 
GACTCTGACA GCAAGTCTGA GGTTGAAGCT CTGGCTGAAC AACTGAGTGA GGAAGAGGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAA GAGGAGGAGG AGGAGGAGGA AGAAGAAGAG 
GAAGATGAAG AGTCTGGCAA TCAGTCTGAC AGGAGTGGTT CCAGCGGCCG CCGCAAAGCC 
AAGAAGAAAT GGCGGAAGGA CAGCCCATGG GTGAAGCCAT CACGGAAACG GCGGAAACGA 
GAGCCTCCGA GGGCCAAGGA GCCACGAGGA GTGAATGGTG TGGGCTCCTC AGGCCCCAGT 
GAGTACATGG AGGTCCCTCT GGGGTCTCTG GAGCTGCCCA GCGAGGGGAC CCTCTCTCCC 
AACCACGCTG GGGTGTCCAA TGACACATCT TCGCTGGAGA CAGAGCGCGG GTTTGAGGAG 
CTGCCCCTCT GCAGCTGCCG CATGGAGGCC CCCAAGATAG ACCGCATCAG CGAGAGAGCA 
GGGCACAAGT GCATGGCCAC AGAGAGCGTG GACGGAGAGC TGTCCGGCTG CAATGCTGCC 
ATCCTCAAGC GGGAGACCAT GAGACCGTCC AGCCGTGTGG CCCTGATGGT GCTCTGTGAG 
ACCCACCGTG CGCGCATGGT CAAACATCAC TGCTGCCCCG GCTGTGGCTA CTTCTGTACG 
GCGGGCACCT TCCTGGAGTG TCACCCCGAC TTCCGTGTGG CCCACCGCTT CCACAAGGCC 
TGTGTGTCCC AGCTGAACGG GATGGTCTTC TGTCCCCACT GTGGGGAGGA CGCATCTGAG 
GCCCAGGAGG TGACCATCCC CCGGGGGGAT GGGGTGACCC CACCGGCTGG CACTGCAGCA 
CCCGCTCCCC CACCCCTGGC CCAGGATGCC CCCGGGAGAG CGGACACTTC CCAGCCCAGT 
GCCCGGATGC GGGGGCAGGG GGAGCCTCGG CGTCCACCCT GCGACCCTCT GGCCGACACC 
ATCGACAGCT CAGGGCCCTC CCTAACCCTG CCCAATGGGG GCTGCCTCTC AGCTGTAGGG 
CTGCCACCAG GGCCAGGCCG GGAGGCCCTG GAGAAGGCCC TGGTCATCCA GGAGTCAGAG 
AGGCGGAAAA AGCTCCGGTT CCACCCTCGG CAGTTGTACC TGTCCGTAAA GCAAGGGGAG 
CTGCAGAAGG TGATCCTGAT GCTGTTGGAC AACCTGGACC CCAACTTCCA GAGTGACCAG 
CAGAGCAAGC GCACACCCCT GCACGCGGCT GCCCAGAAGG GCTCTGTGGA GATCTGCCAT 
GTGCTGCTGC AGGCTGGAGC CAACATCAAT GCCGTGGACA AGCAGCAGCG GACGCCGCTG 
ATGGAGGCCG TGGTGAACAA CCATCTGGAG GTGGCTCGCT ACATGGTGCA GCGTGGGGGC 
TGTGTCTACA GCAAGGAGGA AGACGGCTCC ACCTGCCTCC ACCACGCCGC CAAAATTGGG 
AACCTGGAGA TGGTCAGCCT GCTGCTGAGC ACGGGACAGG TGGACGTCAA TGCCCAGGAC 
AGCGGCGGGT GGACCCCCAT CATCTGGGCT GCAGAGCACA AGCACATAGA AGTGATCCGC 
ATGCTTCTGA CACGGGGCGC GGACGTCACT CTCACCGACA ACGAGGAGAA CATCTGCCTG 
CACTGGGCCT CCTTCACCGG CAGCGCCGCC ATCGCCGAGG TCCTCCTGAA CGCCCGCTGC 
GACCTCCACG CTGTCAATTA CCACGGGGAC ACACCCCTGC ACATCGCGGC CCGGGAGAGC 
TACCACGACT GCGTGCTGTT GTTCCTGTCA CGCGGGGCGA ACCCTGAGCT GCGGAACAAG 
GAGGGGGACA CGGCGTGGGA CCTGACCCCC GAGCGGTCCG ACGTGTGGTT TGCCCTCCAG 
CTCAACCGCA AGCTCCGGCT CGGGGTGGGG AATCGGGCCA TCCGCACGGA GAAGATCATC 
TGCCGGGACG TGGCTCGGGG CTATGAGAAC GTCCCCATCC CCTGTGTCAA TGGTGTGGAC 
GGGGAGCCCT GCCCCGAGGA TTACAAGTAC ATCTCAGAGA ACTGCGAGAC GTCCACCATG 
AACATTGACC GCAACATTAC CCACCTGCAG CACTGCACGT GTGTGGATGA CTGCTCCAGC 
TCCAACTGCC TGTGTGGCCA GCTCAGCATC CGCTGTTGGT ATGACAAGGA TGGGCGGCTG 
CTCCAGGAAT TTAACAAGAT CGAGCCCCCG CTGATTTTTG AGTGTAACCA GGCATGTTCC 
TGCTGGAGAA ACTGCAAGAA CAGAGTAGTG CAGAGCGGCA TCAAGGTGCG ACTGCAGCTC 
TACCGAACAG CCAAGATGGG CTGGGGGGTC CGTGCCCTGC AGACCATTCC CCAGGGTACC 
TTCATTTGCG AGTATGTCGG AGAACTGATC TCTGATGCCG AGGCTGATGT GAGAGAGGAC 
GATTCTTATC TCTTCGACTT GGACAACAAG GATGGAGAGG TGTACTGCAT CGATGCCCGT 
TACTACGGCA ACATCAGCCG CTTCATCAAC CACTTGTGTG ACCCCAACAT CATCCCCGTC 
CGAGTCTTCA TGCTGCACCA AGACCTGAGA TTTCCACGCA TCGCCTTCTT CAGTTCCCGA 
GACATCCGGG CCGGGGAGGA GCTGGGGTTT GACTATGGTG ACCGCTTCTG GGACATCAAA 
AGCAAGTACT TCACCTGCCA GTGTGGCTCT GAGAAGTGCA AGCACTCGGC TGAGGCCATT 
GCCCTGGAGC AGAGCCGCCT GGCCCGCCTG GACCCACACC CTGAGCTGCT GCCTGAGCTC 
AGCTCCCTGC CCCCCGTCAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839340.1
CDS	16..3822
Translation	MRGLPRGRGLMRARGRGRAAPPGSRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPTGLTSPPIPCVPSQGEAPAEMGALVLEKEPRGATERVHGSLGDTSRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	XM_024983572.1
Organism	Bos taurus(cattle)
Definition	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983572.1

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGTTG ATGCGGGCCC GGGGGAGGGG TCGTGCGGCC 
CCTCCGGGCA GCCGAGGCCG CGGAAGGGGG GGAGCCCATA GAGGAAGAGG TAGGCCCCGG 
AGCCTGCTCT CTCTACCCAG GGCCCAGGCG TCCTGGGCCC CCCAGCTTCC TACCGGACTG 
ACCAGCCCTC CTATCCCTTG TGTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGGTGCTGG AGAAGGAGCC CAGAGGAGCC ACCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCGTA GTGAGGAGAC CCTGCCCAAG GCCAACCCCG ATTCCCTGGA GACTGCTGGC 
CCCTCATCCC CAGCCTCTGT CACAGTCACT GTCGGCGATG AGGGGGCTGA CACCCCTGTA 
GGGGCCACAC CACTCATTGG GGATGAACCC GAGAATCTCG AGGGAGATGG GGACCTCCAT 
GGGGGTCGCA TCCTGCTGGG CCATGCCACA AAGTCGTTCC CGTCTTCCCC CAGCAAGGGG 
GGTGCCTGTC CCAGCCGAGC CAAGATGTCA ATGACAGGGG CCGGAAAATC ACCCCCATCA 
GTTCAGAGTT TGGCTATGAG GCTGCTAAGT ATGCCGGGCG CCCAAGGGGC GCCAGCAGCA 
GGGCCTGAAC CCCCTCCGGC CACAGCCAGC CCAGAGGGGC AGCCCAAGGT TCATCGAGCC 
AGGAAAACCA TGTCCAAACC AGGAAATGGA CAGCCCCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTGCAGC ATTTCCGCAT GAGTGATGAC GTGCACTCGC TGGGGAAGGT GACCTCAGAT 
GTGGCCAAAA GGAGGAAGCT GAACTCAGGA GGTGGCTTGT CAGAAGAGTT GGCTTCTACG 
CGGAGTTCGG GAGAAGTGAC CCTGGAGAAG GGGGACCCCG GGTCCCTGGA GGAGTGGGAA 
ACGGTGGTAG GGGATGACTT CAGCCTCTAC TATGATTCCT ACTCTGTGGA TGAGCGGGTG 
GACTCTGACA GCAAGTCTGA GGTTGAAGCT CTGGCTGAAC AACTGAGTGA GGAAGAGGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAA GAGGAGGAGG AGGAGGAGGA AGAAGAAGAG 
GAAGATGAAG AGTCTGGCAA TCAGTCTGAC AGGAGTGGTT CCAGCGGCCG CCGCAAAGCC 
AAGAAGAAAT GGCGGAAGGA CAGCCCATGG GTGAAGCCAT CACGGAAACG GCGGAAACGA 
GAGCCTCCGA GGGCCAAGGA GCCACGAGGA GTGAATGGTG TGGGCTCCTC AGGCCCCAGT 
GAGTACATGG AGGTCCCTCT GGGGTCTCTG GAGCTGCCCA GCGAGGGGAC CCTCTCTCCC 
AACCACGCTG GGGTGTCCAA TGACACATCT TCGCTGGAGA CAGAGCGCGG GTTTGAGGAG 
CTGCCCCTCT GCAGCTGCCG CATGGAGGCC CCCAAGATAG ACCGCATCAG CGAGAGAGCA 
GGGCACAAGT GCATGGCCAC AGAGAGCGTG GACGGAGAGC TGTCCGGCTG CAATGCTGCC 
ATCCTCAAGC GGGAGACCAT GAGACCGTCC AGCCGTGTGG CCCTGATGGT GCTCTGTGAG 
ACCCACCGTG CGCGCATGGT CAAACATCAC TGCTGCCCCG GCTGTGGCTA CTTCTGTACG 
GCGGGCACCT TCCTGGAGTG TCACCCCGAC TTCCGTGTGG CCCACCGCTT CCACAAGGCC 
TGTGTGTCCC AGCTGAACGG GATGGTCTTC TGTCCCCACT GTGGGGAGGA CGCATCTGAG 
GCCCAGGAGG TGACCATCCC CCGGGGGGAT GGGGTGACCC CACCGGCTGG CACTGCAGCA 
CCCGCTCCCC CACCCCTGGC CCAGGATGCC CCCGGGAGAG CGGACACTTC CCAGCCCAGT 
GCCCGGATGC GGGGGCAGGG GGAGCCTCGG CGTCCACCCT GCGACCCTCT GGCCGACACC 
ATCGACAGCT CAGGGCCCTC CCTAACCCTG CCCAATGGGG GCTGCCTCTC AGCTGTAGGG 
CTGCCACCAG GGCCAGGCCG GGAGGCCCTG GAGAAGGCCC TGGTCATCCA GGAGTCAGAG 
AGGCGGAAAA AGCTCCGGTT CCACCCTCGG CAGTTGTACC TGTCCGTAAA GCAAGGGGAG 
CTGCAGAAGG TGATCCTGAT GCTGTTGGAC AACCTGGACC CCAACTTCCA GAGTGACCAG 
CAGAGCAAGC GCACACCCCT GCACGCGGCT GCCCAGAAGG GCTCTGTGGA GATCTGCCAT 
GTGCTGCTGC AGGCTGGAGC CAACATCAAT GCCGTGGACA AGCAGCAGCG GACGCCGCTG 
ATGGAGGCCG TGGTGAACAA CCATCTGGAG GTGGCTCGCT ACATGGTGCA GCGTGGGGGC 
TGTGTCTACA GCAAGGAGGA AGACGGCTCC ACCTGCCTCC ACCACGCCGC CAAAATTGGG 
AACCTGGAGA TGGTCAGCCT GCTGCTGAGC ACGGGACAGG TGGACGTCAA TGCCCAGGAC 
AGCGGCGGGT GGACCCCCAT CATCTGGGCT GCAGAGCACA AGCACATAGA AGTGATCCGC 
ATGCTTCTGA CACGGGGCGC GGACGTCACT CTCACCGACA ACGAGGAGAA CATCTGCCTG 
CACTGGGCCT CCTTCACCGG CAGCGCCGCC ATCGCCGAGG TCCTCCTGAA CGCCCGCTGC 
GACCTCCACG CTGTCAATTA CCACGGGGAC ACACCCCTGC ACATCGCGGC CCGGGAGAGC 
TACCACGACT GCGTGCTGTT GTTCCTGTCA CGCGGGGCGA ACCCTGAGCT GCGGAACAAG 
GAGGGGGACA CGGCGTGGGA CCTGACCCCC GAGCGGTCCG ACGTGTGGTT TGCCCTCCAG 
CTCAACCGCA AGCTCCGGCT CGGGGTGGGG AATCGGGCCA TCCGCACGGA GAAGATCATC 
TGCCGGGACG TGGCTCGGGG CTATGAGAAC GTCCCCATCC CCTGTGTCAA TGGTGTGGAC 
GGGGAGCCCT GCCCCGAGGA TTACAAGTAC ATCTCAGAGA ACTGCGAGAC GTCCACCATG 
AACATTGACC GCAACATTAC CCACCTGCAG CACTGCACGT GTGTGGATGA CTGCTCCAGC 
TCCAACTGCC TGTGTGGCCA GCTCAGCATC CGCTGTTGGT ATGACAAGGA TGGGCGGCTG 
CTCCAGGAAT TTAACAAGAT CGAGCCCCCG CTGATTTTTG AGTGTAACCA GGCATGTTCC 
TGCTGGAGAA ACTGCAAGAA CAGAGTAGTG CAGAGCGGCA TCAAGGTGCG ACTGCAGCTC 
TACCGAACAG CCAAGATGGG CTGGGGGGTC CGTGCCCTGC AGACCATTCC CCAGGGTACC 
TTCATTTGCG AGTATGTCGG AGAACTGATC TCTGATGCCG AGGCTGATGT GAGAGAGGAC 
GATTCTTATC TCTTCGACTT GGACAACAAG GATGGAGAGG TGTACTGCAT CGATGCCCGT 
TACTACGGCA ACATCAGCCG CTTCATCAAC CACTTGTGTG ACCCCAACAT CATCCCCGTC 
CGAGTCTTCA TGCTGCACCA AGACCTGAGA TTTCCACGCA TCGCCTTCTT CAGTTCCCGA 
GACATCCGGG CCGGGGAGGA GCTGGGGTTT GACTATGGTG ACCGCTTCTG GGACATCAAA 
AGCAAGTACT TCACCTGCCA GTGTGGCTCT GAGAAGTGCA AGCACTCGGC TGAGGCCATT 
GCCCTGGAGC AGAGCCGCCT GGCCCGCCTG GACCCACACC CTGAGCTGCT GCCTGAGCTC 
AGCTCCCTGC CCCCCGTCAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHMT2 ( XM_024983573.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024839341.1 Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229478
Clone ID Related Accession (Same CDS sequence)	XM_024983573.1
Accession Version	XM_024983573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase EHMT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGTTG ATGCGGGCCC GGGGGAGGGG TCGTGCGGCC 
CCTCCGGGCA GCCGAGGCCG CGGAAGGGGG GGAGCCCATA GAGGAAGAGG TAGGCCCCGG 
AGCCTGCTCT CTCTACCCAG GGCCCAGGCG TCCTGGGCCC CCCAGCTTCC TACCGGACTG 
ACCAGCCCTC CTATCCCTTG TGTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGGTGCTGG AGAAGGAGCC CAGAGGAGCC ACCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCGTA GTGAGGAGAC CCTGCCCAAG GCCAACCCCG ATTCCCTGGA GACTGCTGGC 
CCCTCATCCC CAGCCTCTGT CACAGTCACT GTCGGCGATG AGGGGGCTGA CACCCCTGTA 
GGGGCCACAC CACTCATTGG GGATGAACCC GAGAATCTCG AGGGAGATGG GGACCTCCAT 
GGGGGTCGCA TCCTGCTGGG CCATGCCACA AAGTCGTTCC CGTCTTCCCC CAGCAAGGGG 
GGTGCCTGTC CCAGCCGAGC CAAGATGTCA ATGACAGGGG CCGGAAAATC ACCCCCATCA 
GTTCAGAGTT TGGCTATGAG GCTGCTAAGT ATGCCGGGCG CCCAAGGGGC GCCAGCAGCA 
GGGCCTGAAC CCCCTCCGGC CACAGCCAGC CCAGAGGGGC AGCCCAAGGT TCATCGAGCC 
AGGAAAACCA TGTCCAAACC AGGAAATGGA CAGCCCCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTGCAGC ATTTCCGCAT GAGTGATGAC GTGCACTCGC TGGGGAAGGT GACCTCAGAT 
GTGGCCAAAA GGAGGAAGCT GAACTCAGGA GGTGGCTTGT CAGAAGAGTT GGCTTCTACG 
CGGAGTTCGG GAGAAGTGAC CCTGGAGAAG GGGGACCCCG GGTCCCTGGA GGAGTGGGAA 
ACGGTGGTAG GGGATGACTT CAGCCTCTAC TATGATTCCT ACTCTGTGGA TGAGCGGGTG 
GACTCTGACA GCAAGTCTGA GGTTGAAGCT CTGGCTGAAC AACTGAGTGA GGAAGAGGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAA GAGGAGGAGG AGGAGGAGGA AGAAGAAGAG 
GAAGATGAAG AGTCTGGCAA TCAGTCTGAC AGGAGTGGTT CCAGCGGCCG CCGCAAAGCC 
AAGAAGAAAT GGCGGAAGGA CAGCCCATGG GTGAAGCCAT CACGGAAACG GCGGAAACGA 
GAGCCTCCGA GGGCCAAGGA GCCACGAGGG GTGTCCAATG ACACATCTTC GCTGGAGACA 
GAGCGCGGGT TTGAGGAGCT GCCCCTCTGC AGCTGCCGCA TGGAGGCCCC CAAGATAGAC 
CGCATCAGCG AGAGAGCAGG GCACAAGTGC ATGGCCACAG AGAGCGTGGA CGGAGAGCTG 
TCCGGCTGCA ATGCTGCCAT CCTCAAGCGG GAGACCATGA GACCGTCCAG CCGTGTGGCC 
CTGATGGTGC TCTGTGAGAC CCACCGTGCG CGCATGGTCA AACATCACTG CTGCCCCGGC 
TGTGGCTACT TCTGTACGGC GGGCACCTTC CTGGAGTGTC ACCCCGACTT CCGTGTGGCC 
CACCGCTTCC ACAAGGCCTG TGTGTCCCAG CTGAACGGGA TGGTCTTCTG TCCCCACTGT 
GGGGAGGACG CATCTGAGGC CCAGGAGGTG ACCATCCCCC GGGGGGATGG GGTGACCCCA 
CCGGCTGGCA CTGCAGCACC CGCTCCCCCA CCCCTGGCCC AGGATGCCCC CGGGAGAGCG 
GACACTTCCC AGCCCAGTGC CCGGATGCGG GGGCAGGGGG AGCCTCGGCG TCCACCCTGC 
GACCCTCTGG CCGACACCAT CGACAGCTCA GGGCCCTCCC TAACCCTGCC CAATGGGGGC 
TGCCTCTCAG CTGTAGGGCT GCCACCAGGG CCAGGCCGGG AGGCCCTGGA GAAGGCCCTG 
GTCATCCAGG AGTCAGAGAG GCGGAAAAAG CTCCGGTTCC ACCCTCGGCA GTTGTACCTG 
TCCGTAAAGC AAGGGGAGCT GCAGAAGGTG ATCCTGATGC TGTTGGACAA CCTGGACCCC 
AACTTCCAGA GTGACCAGCA GAGCAAGCGC ACACCCCTGC ACGCGGCTGC CCAGAAGGGC 
TCTGTGGAGA TCTGCCATGT GCTGCTGCAG GCTGGAGCCA ACATCAATGC CGTGGACAAG 
CAGCAGCGGA CGCCGCTGAT GGAGGCCGTG GTGAACAACC ATCTGGAGGT GGCTCGCTAC 
ATGGTGCAGC GTGGGGGCTG TGTCTACAGC AAGGAGGAAG ACGGCTCCAC CTGCCTCCAC 
CACGCCGCCA AAATTGGGAA CCTGGAGATG GTCAGCCTGC TGCTGAGCAC GGGACAGGTG 
GACGTCAATG CCCAGGACAG CGGCGGGTGG ACCCCCATCA TCTGGGCTGC AGAGCACAAG 
CACATAGAAG TGATCCGCAT GCTTCTGACA CGGGGCGCGG ACGTCACTCT CACCGACAAC 
GAGGAGAACA TCTGCCTGCA CTGGGCCTCC TTCACCGGCA GCGCCGCCAT CGCCGAGGTC 
CTCCTGAACG CCCGCTGCGA CCTCCACGCT GTCAATTACC ACGGGGACAC ACCCCTGCAC 
ATCGCGGCCC GGGAGAGCTA CCACGACTGC GTGCTGTTGT TCCTGTCACG CGGGGCGAAC 
CCTGAGCTGC GGAACAAGGA GGGGGACACG GCGTGGGACC TGACCCCCGA GCGGTCCGAC 
GTGTGGTTTG CCCTCCAGCT CAACCGCAAG CTCCGGCTCG GGGTGGGGAA TCGGGCCATC 
CGCACGGAGA AGATCATCTG CCGGGACGTG GCTCGGGGCT ATGAGAACGT CCCCATCCCC 
TGTGTCAATG GTGTGGACGG GGAGCCCTGC CCCGAGGATT ACAAGTACAT CTCAGAGAAC 
TGCGAGACGT CCACCATGAA CATTGACCGC AACATTACCC ACCTGCAGCA CTGCACGTGT 
GTGGATGACT GCTCCAGCTC CAACTGCCTG TGTGGCCAGC TCAGCATCCG CTGTTGGTAT 
GACAAGGATG GGCGGCTGCT CCAGGAATTT AACAAGATCG AGCCCCCGCT GATTTTTGAG 
TGTAACCAGG CATGTTCCTG CTGGAGAAAC TGCAAGAACA GAGTAGTGCA GAGCGGCATC 
AAGGTGCGAC TGCAGCTCTA CCGAACAGCC AAGATGGGCT GGGGGGTCCG TGCCCTGCAG 
ACCATTCCCC AGGGTACCTT CATTTGCGAG TATGTCGGAG AACTGATCTC TGATGCCGAG 
GCTGATGTGA GAGAGGACGA TTCTTATCTC TTCGACTTGG ACAACAAGGA TGGAGAGGTG 
TACTGCATCG ATGCCCGTTA CTACGGCAAC ATCAGCCGCT TCATCAACCA CTTGTGTGAC 
CCCAACATCA TCCCCGTCCG AGTCTTCATG CTGCACCAAG ACCTGAGATT TCCACGCATC 
GCCTTCTTCA GTTCCCGAGA CATCCGGGCC GGGGAGGAGC TGGGGTTTGA CTATGGTGAC 
CGCTTCTGGG ACATCAAAAG CAAGTACTTC ACCTGCCAGT GTGGCTCTGA GAAGTGCAAG 
CACTCGGCTG AGGCCATTGC CCTGGAGCAG AGCCGCCTGG CCCGCCTGGA CCCACACCCT 
GAGCTGCTGC CTGAGCTCAG CTCCCTGCCC CCCGTCAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839341.1
CDS	16..3720
Translation	MRGLPRGRGLMRARGRGRAAPPGSRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPTGLTSPPIPCVPSQGEAPAEMGALVLEKEPRGATERVHGSLGDTSRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	XM_024983573.1
Organism	Bos taurus(cattle)
Definition	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983573.1

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGTTG ATGCGGGCCC GGGGGAGGGG TCGTGCGGCC 
CCTCCGGGCA GCCGAGGCCG CGGAAGGGGG GGAGCCCATA GAGGAAGAGG TAGGCCCCGG 
AGCCTGCTCT CTCTACCCAG GGCCCAGGCG TCCTGGGCCC CCCAGCTTCC TACCGGACTG 
ACCAGCCCTC CTATCCCTTG TGTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGGTGCTGG AGAAGGAGCC CAGAGGAGCC ACCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCGTA GTGAGGAGAC CCTGCCCAAG GCCAACCCCG ATTCCCTGGA GACTGCTGGC 
CCCTCATCCC CAGCCTCTGT CACAGTCACT GTCGGCGATG AGGGGGCTGA CACCCCTGTA 
GGGGCCACAC CACTCATTGG GGATGAACCC GAGAATCTCG AGGGAGATGG GGACCTCCAT 
GGGGGTCGCA TCCTGCTGGG CCATGCCACA AAGTCGTTCC CGTCTTCCCC CAGCAAGGGG 
GGTGCCTGTC CCAGCCGAGC CAAGATGTCA ATGACAGGGG CCGGAAAATC ACCCCCATCA 
GTTCAGAGTT TGGCTATGAG GCTGCTAAGT ATGCCGGGCG CCCAAGGGGC GCCAGCAGCA 
GGGCCTGAAC CCCCTCCGGC CACAGCCAGC CCAGAGGGGC AGCCCAAGGT TCATCGAGCC 
AGGAAAACCA TGTCCAAACC AGGAAATGGA CAGCCCCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTGCAGC ATTTCCGCAT GAGTGATGAC GTGCACTCGC TGGGGAAGGT GACCTCAGAT 
GTGGCCAAAA GGAGGAAGCT GAACTCAGGA GGTGGCTTGT CAGAAGAGTT GGCTTCTACG 
CGGAGTTCGG GAGAAGTGAC CCTGGAGAAG GGGGACCCCG GGTCCCTGGA GGAGTGGGAA 
ACGGTGGTAG GGGATGACTT CAGCCTCTAC TATGATTCCT ACTCTGTGGA TGAGCGGGTG 
GACTCTGACA GCAAGTCTGA GGTTGAAGCT CTGGCTGAAC AACTGAGTGA GGAAGAGGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAA GAGGAGGAGG AGGAGGAGGA AGAAGAAGAG 
GAAGATGAAG AGTCTGGCAA TCAGTCTGAC AGGAGTGGTT CCAGCGGCCG CCGCAAAGCC 
AAGAAGAAAT GGCGGAAGGA CAGCCCATGG GTGAAGCCAT CACGGAAACG GCGGAAACGA 
GAGCCTCCGA GGGCCAAGGA GCCACGAGGG GTGTCCAATG ACACATCTTC GCTGGAGACA 
GAGCGCGGGT TTGAGGAGCT GCCCCTCTGC AGCTGCCGCA TGGAGGCCCC CAAGATAGAC 
CGCATCAGCG AGAGAGCAGG GCACAAGTGC ATGGCCACAG AGAGCGTGGA CGGAGAGCTG 
TCCGGCTGCA ATGCTGCCAT CCTCAAGCGG GAGACCATGA GACCGTCCAG CCGTGTGGCC 
CTGATGGTGC TCTGTGAGAC CCACCGTGCG CGCATGGTCA AACATCACTG CTGCCCCGGC 
TGTGGCTACT TCTGTACGGC GGGCACCTTC CTGGAGTGTC ACCCCGACTT CCGTGTGGCC 
CACCGCTTCC ACAAGGCCTG TGTGTCCCAG CTGAACGGGA TGGTCTTCTG TCCCCACTGT 
GGGGAGGACG CATCTGAGGC CCAGGAGGTG ACCATCCCCC GGGGGGATGG GGTGACCCCA 
CCGGCTGGCA CTGCAGCACC CGCTCCCCCA CCCCTGGCCC AGGATGCCCC CGGGAGAGCG 
GACACTTCCC AGCCCAGTGC CCGGATGCGG GGGCAGGGGG AGCCTCGGCG TCCACCCTGC 
GACCCTCTGG CCGACACCAT CGACAGCTCA GGGCCCTCCC TAACCCTGCC CAATGGGGGC 
TGCCTCTCAG CTGTAGGGCT GCCACCAGGG CCAGGCCGGG AGGCCCTGGA GAAGGCCCTG 
GTCATCCAGG AGTCAGAGAG GCGGAAAAAG CTCCGGTTCC ACCCTCGGCA GTTGTACCTG 
TCCGTAAAGC AAGGGGAGCT GCAGAAGGTG ATCCTGATGC TGTTGGACAA CCTGGACCCC 
AACTTCCAGA GTGACCAGCA GAGCAAGCGC ACACCCCTGC ACGCGGCTGC CCAGAAGGGC 
TCTGTGGAGA TCTGCCATGT GCTGCTGCAG GCTGGAGCCA ACATCAATGC CGTGGACAAG 
CAGCAGCGGA CGCCGCTGAT GGAGGCCGTG GTGAACAACC ATCTGGAGGT GGCTCGCTAC 
ATGGTGCAGC GTGGGGGCTG TGTCTACAGC AAGGAGGAAG ACGGCTCCAC CTGCCTCCAC 
CACGCCGCCA AAATTGGGAA CCTGGAGATG GTCAGCCTGC TGCTGAGCAC GGGACAGGTG 
GACGTCAATG CCCAGGACAG CGGCGGGTGG ACCCCCATCA TCTGGGCTGC AGAGCACAAG 
CACATAGAAG TGATCCGCAT GCTTCTGACA CGGGGCGCGG ACGTCACTCT CACCGACAAC 
GAGGAGAACA TCTGCCTGCA CTGGGCCTCC TTCACCGGCA GCGCCGCCAT CGCCGAGGTC 
CTCCTGAACG CCCGCTGCGA CCTCCACGCT GTCAATTACC ACGGGGACAC ACCCCTGCAC 
ATCGCGGCCC GGGAGAGCTA CCACGACTGC GTGCTGTTGT TCCTGTCACG CGGGGCGAAC 
CCTGAGCTGC GGAACAAGGA GGGGGACACG GCGTGGGACC TGACCCCCGA GCGGTCCGAC 
GTGTGGTTTG CCCTCCAGCT CAACCGCAAG CTCCGGCTCG GGGTGGGGAA TCGGGCCATC 
CGCACGGAGA AGATCATCTG CCGGGACGTG GCTCGGGGCT ATGAGAACGT CCCCATCCCC 
TGTGTCAATG GTGTGGACGG GGAGCCCTGC CCCGAGGATT ACAAGTACAT CTCAGAGAAC 
TGCGAGACGT CCACCATGAA CATTGACCGC AACATTACCC ACCTGCAGCA CTGCACGTGT 
GTGGATGACT GCTCCAGCTC CAACTGCCTG TGTGGCCAGC TCAGCATCCG CTGTTGGTAT 
GACAAGGATG GGCGGCTGCT CCAGGAATTT AACAAGATCG AGCCCCCGCT GATTTTTGAG 
TGTAACCAGG CATGTTCCTG CTGGAGAAAC TGCAAGAACA GAGTAGTGCA GAGCGGCATC 
AAGGTGCGAC TGCAGCTCTA CCGAACAGCC AAGATGGGCT GGGGGGTCCG TGCCCTGCAG 
ACCATTCCCC AGGGTACCTT CATTTGCGAG TATGTCGGAG AACTGATCTC TGATGCCGAG 
GCTGATGTGA GAGAGGACGA TTCTTATCTC TTCGACTTGG ACAACAAGGA TGGAGAGGTG 
TACTGCATCG ATGCCCGTTA CTACGGCAAC ATCAGCCGCT TCATCAACCA CTTGTGTGAC 
CCCAACATCA TCCCCGTCCG AGTCTTCATG CTGCACCAAG ACCTGAGATT TCCACGCATC 
GCCTTCTTCA GTTCCCGAGA CATCCGGGCC GGGGAGGAGC TGGGGTTTGA CTATGGTGAC 
CGCTTCTGGG ACATCAAAAG CAAGTACTTC ACCTGCCAGT GTGGCTCTGA GAAGTGCAAG 
CACTCGGCTG AGGCCATTGC CCTGGAGCAG AGCCGCCTGG CCCGCCTGGA CCCACACCCT 
GAGCTGCTGC CTGAGCTCAG CTCCCTGCCC CCCGTCAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHMT2 ( NM_001206263.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001193192.2 Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), mRNA.

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CloneID	OBa216189
Clone ID Related Accession (Same CDS sequence)	NM_001206263.2
Accession Version	NM_001206263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase EHMT2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000023.1. On Jun 30, 2017 this sequence version replaced NM_001206263.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.22402.1, SRR5571254.16668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGAG TGAATGGTGT GGGCTCCTCA 
GGCCCCAGTG AGTACATGGA GGTCCCTCTG GGGTCTCTGG AGCTGCCCAG CGAGGGGACC 
CTCTCTCCCA ACCACGCTGG GGTGTCCAAT GACACATCTT CGCTGGAGAC AGAGCGCGGG 
TTTGAGGAGC TGCCCCTCTG CAGCTGCCGC ATGGAGGCCC CCAAGATAGA CCGCATCAGC 
GAGAGAGCAG GGCACAAGTG CATGGCCACA GAGAGCGTGG ACGGAGAGCT GTCCGGCTGC 
AATGCTGCCA TCCTCAAGCG GGAGACCATG AGACCGTCCA GCCGTGTGGC CCTGATGGTG 
CTCTGTGAGA CCCACCGTGC GCGCATGGTC AAACATCACT GCTGCCCCGG CTGTGGCTAC 
TTCTGTACGG CGGGCACCTT CCTGGAGTGT CACCCCGACT TCCGTGTGGC CCACCGCTTC 
CACAAGGCCT GTGTGTCCCA GCTGAACGGG ATGGTCTTCT GTCCCCACTG TGGGGAGGAC 
GCATCTGAGG CCCAGGAGGT GACCATCCCC CGGGGGGATG GGGTGACCCC ACCGGCTGGC 
ACTGCAGCAC CCGCTCCCCC ACCCCTGGCC CAGGATGCCC CCGGGAGAGC GGACACTTCC 
CAGCCCAGTG CCCGGATGCG GGGGCAGGGG GAGCCTCGGC GTCCACCCTG CGACCCTCTG 
GCCGACACCA TCGACAGCTC AGGGCCCTCC CTAACCCTGC CCAATGGGGG CTGCCTCTCA 
GCTGTAGGGC TGCCACCAGG GCCAGGCCGG GAGGCCCTGG AGAAGGCCCT GGTCATCCAG 
GAGTCAGAGA GGCGGAAAAA GCTCCGGTTC CACCCTCGGC AGTTGTACCT GTCCGTAAAG 
CAAGGGGAGC TGCAGAAGGT GATCCTGATG CTGTTGGACA ACCTGGACCC CAACTTCCAG 
AGTGACCAGC AGAGCAAGCG CACACCCCTG CACGCGGCTG CCCAGAAGGG CTCTGTGGAG 
ATCTGCCATG TGCTGCTGCA GGCTGGAGCC AACATCAATG CCGTGGACAA GCAGCAGCGG 
ACGCCGCTGA TGGAGGCCGT GGTGAACAAC CATCTGGAGG TGGCTCGCTA CATGGTGCAG 
CGTGGGGGCT GTGTCTACAG CAAGGAGGAA GACGGCTCCA CCTGCCTCCA CCACGCCGCC 
AAAATTGGGA ACCTGGAGAT GGTCAGCCTG CTGCTGAGCA CGGGACAGGT GGACGTCAAT 
GCCCAGGACA GCGGCGGGTG GACCCCCATC ATCTGGGCTG CAGAGCACAA GCACATAGAA 
GTGATCCGCA TGCTTCTGAC ACGGGGCGCG GACGTCACTC TCACCGACAA CGAGGAGAAC 
ATCTGCCTGC ACTGGGCCTC CTTCACCGGC AGCGCCGCCA TCGCCGAGGT CCTCCTGAAC 
GCCCGCTGCG ACCTCCACGC TGTCAATTAC CACGGGGACA CACCCCTGCA CATCGCGGCC 
CGGGAGAGCT ACCACGACTG CGTGCTGTTG TTCCTGTCAC GCGGGGCGAA CCCTGAGCTG 
CGGAACAAGG AGGGGGACAC GGCGTGGGAC CTGACCCCCG AGCGGTCCGA CGTGTGGTTT 
GCCCTCCAGC TCAACCGCAA GCTCCGGCTC GGGGTGGGGA ATCGGGCCAT CCGCACGGAG 
AAGATCATCT GCCGGGACGT GGCTCGGGGC TATGAGAACG TCCCCATCCC CTGTGTCAAT 
GGTGTGGACG GGGAGCCCTG CCCCGAGGAT TACAAGTACA TCTCAGAGAA CTGCGAGACG 
TCCACCATGA ACATTGACCG CAACATTACC CACCTGCAGC ACTGCACGTG TGTGGATGAC 
TGCTCCAGCT CCAACTGCCT GTGTGGCCAG CTCAGCATCC GCTGTTGGTA TGACAAGGAT 
GGGCGGCTGC TCCAGGAATT TAACAAGATC GAGCCCCCGC TGATTTTTGA GTGTAACCAG 
GCATGTTCCT GCTGGAGAAA CTGCAAGAAC AGAGTAGTGC AGAGCGGCAT CAAGGTGCGA 
CTGCAGCTCT ACCGAACAGC CAAGATGGGC TGGGGGGTCC GTGCCCTGCA GACCATTCCC 
CAGGGTACCT TCATTTGCGA GTATGTCGGA GAACTGATCT CTGATGCCGA GGCTGATGTG 
AGAGAGGACG ATTCTTATCT CTTCGACTTG GACAACAAGG ATGGAGAGGT GTACTGCATC 
GATGCCCGTT ACTACGGCAA CATCAGCCGC TTCATCAACC ACTTGTGTGA CCCCAACATC 
ATCCCCGTCC GAGTCTTCAT GCTGCACCAA GACCTGAGAT TTCCACGCAT CGCCTTCTTC 
AGTTCCCGAG ACATCCGGGC CGGGGAGGAG CTGGGGTTTG ACTATGGTGA CCGCTTCTGG 
GACATCAAAA GCAAGTACTT CACCTGCCAG TGTGGCTCTG AGAAGTGCAA GCACTCGGCT 
GAGGCCATTG CCCTGGAGCA GAGCCGCCTG GCCCGCCTGG ACCCACACCC TGAGCTGCTG 
CCTGAGCTCA GCTCCCTGCC CCCCGTCAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193192.2
CDS	1..3636
Translation	MAAAAGAAAAAAAEGEAPAEMGALVLEKEPRGATERVHGSLGDTSRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	NM_001206263.2
Organism	Bos taurus(cattle)
Definition	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206263.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGAG TGAATGGTGT GGGCTCCTCA 
GGCCCCAGTG AGTACATGGA GGTCCCTCTG GGGTCTCTGG AGCTGCCCAG CGAGGGGACC 
CTCTCTCCCA ACCACGCTGG GGTGTCCAAT GACACATCTT CGCTGGAGAC AGAGCGCGGG 
TTTGAGGAGC TGCCCCTCTG CAGCTGCCGC ATGGAGGCCC CCAAGATAGA CCGCATCAGC 
GAGAGAGCAG GGCACAAGTG CATGGCCACA GAGAGCGTGG ACGGAGAGCT GTCCGGCTGC 
AATGCTGCCA TCCTCAAGCG GGAGACCATG AGACCGTCCA GCCGTGTGGC CCTGATGGTG 
CTCTGTGAGA CCCACCGTGC GCGCATGGTC AAACATCACT GCTGCCCCGG CTGTGGCTAC 
TTCTGTACGG CGGGCACCTT CCTGGAGTGT CACCCCGACT TCCGTGTGGC CCACCGCTTC 
CACAAGGCCT GTGTGTCCCA GCTGAACGGG ATGGTCTTCT GTCCCCACTG TGGGGAGGAC 
GCATCTGAGG CCCAGGAGGT GACCATCCCC CGGGGGGATG GGGTGACCCC ACCGGCTGGC 
ACTGCAGCAC CCGCTCCCCC ACCCCTGGCC CAGGATGCCC CCGGGAGAGC GGACACTTCC 
CAGCCCAGTG CCCGGATGCG GGGGCAGGGG GAGCCTCGGC GTCCACCCTG CGACCCTCTG 
GCCGACACCA TCGACAGCTC AGGGCCCTCC CTAACCCTGC CCAATGGGGG CTGCCTCTCA 
GCTGTAGGGC TGCCACCAGG GCCAGGCCGG GAGGCCCTGG AGAAGGCCCT GGTCATCCAG 
GAGTCAGAGA GGCGGAAAAA GCTCCGGTTC CACCCTCGGC AGTTGTACCT GTCCGTAAAG 
CAAGGGGAGC TGCAGAAGGT GATCCTGATG CTGTTGGACA ACCTGGACCC CAACTTCCAG 
AGTGACCAGC AGAGCAAGCG CACACCCCTG CACGCGGCTG CCCAGAAGGG CTCTGTGGAG 
ATCTGCCATG TGCTGCTGCA GGCTGGAGCC AACATCAATG CCGTGGACAA GCAGCAGCGG 
ACGCCGCTGA TGGAGGCCGT GGTGAACAAC CATCTGGAGG TGGCTCGCTA CATGGTGCAG 
CGTGGGGGCT GTGTCTACAG CAAGGAGGAA GACGGCTCCA CCTGCCTCCA CCACGCCGCC 
AAAATTGGGA ACCTGGAGAT GGTCAGCCTG CTGCTGAGCA CGGGACAGGT GGACGTCAAT 
GCCCAGGACA GCGGCGGGTG GACCCCCATC ATCTGGGCTG CAGAGCACAA GCACATAGAA 
GTGATCCGCA TGCTTCTGAC ACGGGGCGCG GACGTCACTC TCACCGACAA CGAGGAGAAC 
ATCTGCCTGC ACTGGGCCTC CTTCACCGGC AGCGCCGCCA TCGCCGAGGT CCTCCTGAAC 
GCCCGCTGCG ACCTCCACGC TGTCAATTAC CACGGGGACA CACCCCTGCA CATCGCGGCC 
CGGGAGAGCT ACCACGACTG CGTGCTGTTG TTCCTGTCAC GCGGGGCGAA CCCTGAGCTG 
CGGAACAAGG AGGGGGACAC GGCGTGGGAC CTGACCCCCG AGCGGTCCGA CGTGTGGTTT 
GCCCTCCAGC TCAACCGCAA GCTCCGGCTC GGGGTGGGGA ATCGGGCCAT CCGCACGGAG 
AAGATCATCT GCCGGGACGT GGCTCGGGGC TATGAGAACG TCCCCATCCC CTGTGTCAAT 
GGTGTGGACG GGGAGCCCTG CCCCGAGGAT TACAAGTACA TCTCAGAGAA CTGCGAGACG 
TCCACCATGA ACATTGACCG CAACATTACC CACCTGCAGC ACTGCACGTG TGTGGATGAC 
TGCTCCAGCT CCAACTGCCT GTGTGGCCAG CTCAGCATCC GCTGTTGGTA TGACAAGGAT 
GGGCGGCTGC TCCAGGAATT TAACAAGATC GAGCCCCCGC TGATTTTTGA GTGTAACCAG 
GCATGTTCCT GCTGGAGAAA CTGCAAGAAC AGAGTAGTGC AGAGCGGCAT CAAGGTGCGA 
CTGCAGCTCT ACCGAACAGC CAAGATGGGC TGGGGGGTCC GTGCCCTGCA GACCATTCCC 
CAGGGTACCT TCATTTGCGA GTATGTCGGA GAACTGATCT CTGATGCCGA GGCTGATGTG 
AGAGAGGACG ATTCTTATCT CTTCGACTTG GACAACAAGG ATGGAGAGGT GTACTGCATC 
GATGCCCGTT ACTACGGCAA CATCAGCCGC TTCATCAACC ACTTGTGTGA CCCCAACATC 
ATCCCCGTCC GAGTCTTCAT GCTGCACCAA GACCTGAGAT TTCCACGCAT CGCCTTCTTC 
AGTTCCCGAG ACATCCGGGC CGGGGAGGAG CTGGGGTTTG ACTATGGTGA CCGCTTCTGG 
GACATCAAAA GCAAGTACTT CACCTGCCAG TGTGGCTCTG AGAAGTGCAA GCACTCGGCT 
GAGGCCATTG CCCTGGAGCA GAGCCGCCTG GCCCGCCTGG ACCCACACCC TGAGCTGCTG 
CCTGAGCTCA GCTCCCTGCC CCCCGTCAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHMT2 ( NM_001206263.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001193192.1 Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), mRNA.

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CloneID	OBa41249
Clone ID Related Accession (Same CDS sequence)	NM_001206263.1
Accession Version	NM_001206263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	histone-lysine N-methyltransferase EHMT2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03050901.1 and AAFC03050905.1. On Apr 20, 2011 this sequence version replaced XM_869170.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCCCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGAG TGAATGGTGT GGGCTCCTCA 
GGCCCCAGTG AGTACATGGA GGTCCCTCTG GGGTCTCTGG AGCTGCCCAG CGAGGGGACC 
CTCTCTCCCA ACCACGCTGG GGTGTCCAAT GACACATCTT CGCTGGAGAC AGAGCGCGGG 
TTTGAGGAGC TGCCCCTCTG CAGCTGCCGC ATGGAGGCCC CCAAGATAGA CCGCATCAGC 
GAGAGAGCAG GGCACAAGTG CATGGCCACA GAGAGCGTGG ACGGAGAGCT GTCCGGCTGC 
AATGCTGCCA TCCTCAAGCG GGAGACCATG AGACCGTCCA GCCGTGTGGC CCTGATGGTG 
CTCTGTGAGA CCCACCGTGC GCGCATGGTC AAACATCACT GCTGCCCCGG CTGTGGCTAC 
TTCTGTACGG CGGGCACCTT CCTGGAGTGT CACCCCGACT TCCGTGTGGC CCACCGCTTC 
CACAAGGCCT GTGTGTCCCA GCTGAACGGG ATGGTCTTCT GTCCCCACTG TGGGGAGGAC 
GCATCTGAGG CCCAGGAGGT GACCATCCCC CGGGGGGATG GGGTGACCCC ACCGGCTGGC 
ACTGCAGCAC CCGCTCCCCC ACCCCTGGCC CAGGATGCCC CCGGGAGAGC GGACACTTCC 
CAGCCCAGTG CCCGGATGCG GGGGCAGGGG GAGCCTCGGC GTCCACCCTG CGACCCTCTG 
GCCGACACCA TCGACAGCTC AGGGCCCTCC CTAACCCTGC CCAATGGGGG CTGCCTCTCA 
GCTGTAGGGC TGCCACCAGG GCCAGGCCGG GAGGCCCTGG AGAAGGCCCT GGTCATCCAG 
GAGTCAGAGA GGCGGAAAAA GCTCCGGTTC CACCCTCGGC AGTTGTACCT GTCCGTAAAG 
CAAGGGGAGC TGCAGAAGGT GATCCTGATG CTGTTGGACA ACCTGGACCC CAACTTCCAG 
AGTGACCAGC AGAGCAAGCG CACACCCCTG CACGCGGCTG CCCAGAAGGG CTCTGTGGAG 
ATCTGCCATG TGCTGCTGCA GGCTGGAGCC AACATCAATG CCGTGGACAA GCAGCAGCGG 
ACGCCGCTGA TGGAGGCCGT GGTGAACAAC CATCTGGAGG TGGCTCGCTA CATGGTGCAG 
CGTGGGGGCT GTGTCTACAG CAAGGAGGAA GACGGCTCCA CCTGCCTCCA CCACGCCGCC 
AAAATTGGGA ACCTGGAGAT GGTCAGCCTG CTGCTGAGCA CGGGACAGGT GGACGTCAAT 
GCCCAGGACA GCGGCGGGTG GACCCCCATC ATCTGGGCTG CAGAGCACAA GCACATAGAA 
GTGATCCGCA TGCTTCTGAC ACGGGGCGCG GACGTCACTC TCACCGACAA CGAGGAGAAC 
ATCTGCCTGC ACTGGGCCTC CTTCACCGGC AGCGCCGCCA TCGCCGAGGT CCTCCTGAAC 
GCCCGCTGCG ACCTCCACGC TGTCAATTAC CACGGGGACA CACCCCTGCA CATCGCGGCC 
CGGGAGAGCT ACCACGACTG CGTGCTGTTG TTCCTGTCAC GCGGGGCGAA CCCTGAGCTG 
CGGAACAAGG AGGGGGACAC GGCGTGGGAC CTGACCCCCG AGCGGTCCGA CGTGTGGTTT 
GCCCTCCAGC TCAACCGCAA GCTCCGGCTC GGGGTGGGGA ATCGGGCCAT CCGCACGGAG 
AAGATCATCT GCCGGGACGT GGCTCGGGGC TATGAGAACG TCCCCATCCC CTGTGTCAAT 
GGTGTGGACG GGGAGCCCTG CCCCGAGGAT TACAAGTACA TCTCAGAGAA CTGCGAGACG 
TCCACCATGA ACATTGACCG CAACATTACC CACCTGCAGC ACTGCACGTG TGTGGATGAC 
TGCTCCAGCT CCAACTGCCT GTGTGGCCAG CTCAGCATCC GCTGTTGGTA TGACAAGGAT 
GGGCGGCTGC TCCAGGAATT TAACAAGATC GAGCCCCCGC TGATTTTTGA GTGTAACCAG 
GCATGTTCCT GCTGGAGAAA CTGCAAGAAC AGAGTAGTGC AGAGCGGCAT CAAGGTGCGA 
CTGCAGCTCT ACCGAACAGC CAAGATGGGC TGGGGGGTCC GTGCCCTGCA GACCATTCCC 
CAGGGTACCT TCATTTGCGA GTATGTCGGA GAACTGATCT CTGATGCCGA GGCTGATGTG 
AGAGAGGACG ATTCTTATCT CTTCGACTTG GACAACAAGG ATGGAGAGGT GTACTGCATC 
GATGCCCGTT ACTACGGCAA CATCAGCCGC TTCATCAACC ACTTGTGTGA CCCCAACATC 
ATCCCCGTCC GAGTCTTCAT GCTGCACCAA GACCTGAGAT TTCCACGCAT CGCCTTCTTC 
AGTTCCCGAG ACATCCGGGC CGGGGAGGAG CTGGGGTTTG ACTATGGTGA CCGCTTCTGG 
GACATCAAAA GCAAGTACTT CACCTGCCAG TGTGGCTCTG AGAAGTGCAA GCACTCGGCT 
GAGGCCATTG CCCTGGAGCA GAGCCGCCTG GCCCGCCTGG ACCCACACCC TGAGCTGCTG 
CCTGAGCTCA GCTCCCTGCC CCCCGTCAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193192.1
CDS	1..3636
Translation	MAAAAGAAAAAAAEGEAPAEMGALVLEKEPRGATERVHGSLGDTPRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	NM_001206263.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206263.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCCCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGAG TGAATGGTGT GGGCTCCTCA 
GGCCCCAGTG AGTACATGGA GGTCCCTCTG GGGTCTCTGG AGCTGCCCAG CGAGGGGACC 
CTCTCTCCCA ACCACGCTGG GGTGTCCAAT GACACATCTT CGCTGGAGAC AGAGCGCGGG 
TTTGAGGAGC TGCCCCTCTG CAGCTGCCGC ATGGAGGCCC CCAAGATAGA CCGCATCAGC 
GAGAGAGCAG GGCACAAGTG CATGGCCACA GAGAGCGTGG ACGGAGAGCT GTCCGGCTGC 
AATGCTGCCA TCCTCAAGCG GGAGACCATG AGACCGTCCA GCCGTGTGGC CCTGATGGTG 
CTCTGTGAGA CCCACCGTGC GCGCATGGTC AAACATCACT GCTGCCCCGG CTGTGGCTAC 
TTCTGTACGG CGGGCACCTT CCTGGAGTGT CACCCCGACT TCCGTGTGGC CCACCGCTTC 
CACAAGGCCT GTGTGTCCCA GCTGAACGGG ATGGTCTTCT GTCCCCACTG TGGGGAGGAC 
GCATCTGAGG CCCAGGAGGT GACCATCCCC CGGGGGGATG GGGTGACCCC ACCGGCTGGC 
ACTGCAGCAC CCGCTCCCCC ACCCCTGGCC CAGGATGCCC CCGGGAGAGC GGACACTTCC 
CAGCCCAGTG CCCGGATGCG GGGGCAGGGG GAGCCTCGGC GTCCACCCTG CGACCCTCTG 
GCCGACACCA TCGACAGCTC AGGGCCCTCC CTAACCCTGC CCAATGGGGG CTGCCTCTCA 
GCTGTAGGGC TGCCACCAGG GCCAGGCCGG GAGGCCCTGG AGAAGGCCCT GGTCATCCAG 
GAGTCAGAGA GGCGGAAAAA GCTCCGGTTC CACCCTCGGC AGTTGTACCT GTCCGTAAAG 
CAAGGGGAGC TGCAGAAGGT GATCCTGATG CTGTTGGACA ACCTGGACCC CAACTTCCAG 
AGTGACCAGC AGAGCAAGCG CACACCCCTG CACGCGGCTG CCCAGAAGGG CTCTGTGGAG 
ATCTGCCATG TGCTGCTGCA GGCTGGAGCC AACATCAATG CCGTGGACAA GCAGCAGCGG 
ACGCCGCTGA TGGAGGCCGT GGTGAACAAC CATCTGGAGG TGGCTCGCTA CATGGTGCAG 
CGTGGGGGCT GTGTCTACAG CAAGGAGGAA GACGGCTCCA CCTGCCTCCA CCACGCCGCC 
AAAATTGGGA ACCTGGAGAT GGTCAGCCTG CTGCTGAGCA CGGGACAGGT GGACGTCAAT 
GCCCAGGACA GCGGCGGGTG GACCCCCATC ATCTGGGCTG CAGAGCACAA GCACATAGAA 
GTGATCCGCA TGCTTCTGAC ACGGGGCGCG GACGTCACTC TCACCGACAA CGAGGAGAAC 
ATCTGCCTGC ACTGGGCCTC CTTCACCGGC AGCGCCGCCA TCGCCGAGGT CCTCCTGAAC 
GCCCGCTGCG ACCTCCACGC TGTCAATTAC CACGGGGACA CACCCCTGCA CATCGCGGCC 
CGGGAGAGCT ACCACGACTG CGTGCTGTTG TTCCTGTCAC GCGGGGCGAA CCCTGAGCTG 
CGGAACAAGG AGGGGGACAC GGCGTGGGAC CTGACCCCCG AGCGGTCCGA CGTGTGGTTT 
GCCCTCCAGC TCAACCGCAA GCTCCGGCTC GGGGTGGGGA ATCGGGCCAT CCGCACGGAG 
AAGATCATCT GCCGGGACGT GGCTCGGGGC TATGAGAACG TCCCCATCCC CTGTGTCAAT 
GGTGTGGACG GGGAGCCCTG CCCCGAGGAT TACAAGTACA TCTCAGAGAA CTGCGAGACG 
TCCACCATGA ACATTGACCG CAACATTACC CACCTGCAGC ACTGCACGTG TGTGGATGAC 
TGCTCCAGCT CCAACTGCCT GTGTGGCCAG CTCAGCATCC GCTGTTGGTA TGACAAGGAT 
GGGCGGCTGC TCCAGGAATT TAACAAGATC GAGCCCCCGC TGATTTTTGA GTGTAACCAG 
GCATGTTCCT GCTGGAGAAA CTGCAAGAAC AGAGTAGTGC AGAGCGGCAT CAAGGTGCGA 
CTGCAGCTCT ACCGAACAGC CAAGATGGGC TGGGGGGTCC GTGCCCTGCA GACCATTCCC 
CAGGGTACCT TCATTTGCGA GTATGTCGGA GAACTGATCT CTGATGCCGA GGCTGATGTG 
AGAGAGGACG ATTCTTATCT CTTCGACTTG GACAACAAGG ATGGAGAGGT GTACTGCATC 
GATGCCCGTT ACTACGGCAA CATCAGCCGC TTCATCAACC ACTTGTGTGA CCCCAACATC 
ATCCCCGTCC GAGTCTTCAT GCTGCACCAA GACCTGAGAT TTCCACGCAT CGCCTTCTTC 
AGTTCCCGAG ACATCCGGGC CGGGGAGGAG CTGGGGTTTG ACTATGGTGA CCGCTTCTGG 
GACATCAAAA GCAAGTACTT CACCTGCCAG TGTGGCTCTG AGAAGTGCAA GCACTCGGCT 
GAGGCCATTG CCCTGGAGCA GAGCCGCCTG GCCCGCCTGG ACCCACACCC TGAGCTGCTG 
CCTGAGCTCA GCTCCCTGCC CCCCGTCAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHMT2 ( XM_005223636.2 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005223693.1 Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant X1, mRNA.

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CloneID	OBa32136
Clone ID Related Accession (Same CDS sequence)	XM_005223636.2 , XM_005223636.3
Accession Version	XM_005223636.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase EHMT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGGG TGTCCAATGA CACATCTTCG 
CTGGAGACAG AGCGCGGGTT TGAGGAGCTG CCCCTCTGCA GCTGCCGCAT GGAGGCCCCC 
AAGATAGACC GCATCAGCGA GAGAGCAGGG CACAAGTGCA TGGCCACAGA GAGCGTGGAC 
GGAGAGCTGT CCGGCTGCAA TGCTGCCATC CTCAAGCGGG AGACCATGAG ACCGTCCAGC 
CGTGTGGCCC TGATGGTGCT CTGTGAGACC CACCGTGCGC GCATGGTCAA ACATCACTGC 
TGCCCCGGCT GTGGCTACTT CTGTACGGCG GGCACCTTCC TGGAGTGTCA CCCCGACTTC 
CGTGTGGCCC ACCGCTTCCA CAAGGCCTGT GTGTCCCAGC TGAACGGGAT GGTCTTCTGT 
CCCCACTGTG GGGAGGACGC ATCTGAGGCC CAGGAGGTGA CCATCCCCCG GGGGGATGGG 
GTGACCCCAC CGGCTGGCAC TGCAGCACCC GCTCCCCCAC CCCTGGCCCA GGATGCCCCC 
GGGAGAGCGG ACACTTCCCA GCCCAGTGCC CGGATGCGGG GGCAGGGGGA GCCTCGGCGT 
CCACCCTGCG ACCCTCTGGC CGACACCATC GACAGCTCAG GGCCCTCCCT AACCCTGCCC 
AATGGGGGCT GCCTCTCAGC TGTAGGGCTG CCACCAGGGC CAGGCCGGGA GGCCCTGGAG 
AAGGCCCTGG TCATCCAGGA GTCAGAGAGG CGGAAAAAGC TCCGGTTCCA CCCTCGGCAG 
TTGTACCTGT CCGTAAAGCA AGGGGAGCTG CAGAAGGTGA TCCTGATGCT GTTGGACAAC 
CTGGACCCCA ACTTCCAGAG TGACCAGCAG AGCAAGCGCA CACCCCTGCA CGCGGCTGCC 
CAGAAGGGCT CTGTGGAGAT CTGCCATGTG CTGCTGCAGG CTGGAGCCAA CATCAATGCC 
GTGGACAAGC AGCAGCGGAC GCCGCTGATG GAGGCCGTGG TGAACAACCA TCTGGAGGTG 
GCTCGCTACA TGGTGCAGCG TGGGGGCTGT GTCTACAGCA AGGAGGAAGA CGGCTCCACC 
TGCCTCCACC ACGCCGCCAA AATTGGGAAC CTGGAGATGG TCAGCCTGCT GCTGAGCACG 
GGACAGGTGG ACGTCAATGC CCAGGACAGC GGCGGGTGGA CCCCCATCAT CTGGGCTGCA 
GAGCACAAGC ACATAGAAGT GATCCGCATG CTTCTGACAC GGGGCGCGGA CGTCACTCTC 
ACCGACAACG AGGAGAACAT CTGCCTGCAC TGGGCCTCCT TCACCGGCAG CGCCGCCATC 
GCCGAGGTCC TCCTGAACGC CCGCTGCGAC CTCCACGCTG TCAATTACCA CGGGGACACA 
CCCCTGCACA TCGCGGCCCG GGAGAGCTAC CACGACTGCG TGCTGTTGTT CCTGTCACGC 
GGGGCGAACC CTGAGCTGCG GAACAAGGAG GGGGACACGG CGTGGGACCT GACCCCCGAG 
CGGTCCGACG TGTGGTTTGC CCTCCAGCTC AACCGCAAGC TCCGGCTCGG GGTGGGGAAT 
CGGGCCATCC GCACGGAGAA GATCATCTGC CGGGACGTGG CTCGGGGCTA TGAGAACGTC 
CCCATCCCCT GTGTCAATGG TGTGGACGGG GAGCCCTGCC CCGAGGATTA CAAGTACATC 
TCAGAGAACT GCGAGACGTC CACCATGAAC ATTGACCGCA ACATTACCCA CCTGCAGCAC 
TGCACGTGTG TGGATGACTG CTCCAGCTCC AACTGCCTGT GTGGCCAGCT CAGCATCCGC 
TGTTGGTATG ACAAGGATGG GCGGCTGCTC CAGGAATTTA ACAAGATCGA GCCCCCGCTG 
ATTTTTGAGT GTAACCAGGC ATGTTCCTGC TGGAGAAACT GCAAGAACAG AGTAGTGCAG 
AGCGGCATCA AGGTGCGACT GCAGCTCTAC CGAACAGCCA AGATGGGCTG GGGGGTCCGT 
GCCCTGCAGA CCATTCCCCA GGGTACCTTC ATTTGCGAGT ATGTCGGAGA ACTGATCTCT 
GATGCCGAGG CTGATGTGAG AGAGGACGAT TCTTATCTCT TCGACTTGGA CAACAAGGAT 
GGAGAGGTGT ACTGCATCGA TGCCCGTTAC TACGGCAACA TCAGCCGCTT CATCAACCAC 
TTGTGTGACC CCAACATCAT CCCCGTCCGA GTCTTCATGC TGCACCAAGA CCTGAGATTT 
CCACGCATCG CCTTCTTCAG TTCCCGAGAC ATCCGGGCCG GGGAGGAGCT GGGGTTTGAC 
TATGGTGACC GCTTCTGGGA CATCAAAAGC AAGTACTTCA CCTGCCAGTG TGGCTCTGAG 
AAGTGCAAGC ACTCGGCTGA GGCCATTGCC CTGGAGCAGA GCCGCCTGGC CCGCCTGGAC 
CCACACCCTG AGCTGCTGCC TGAGCTCAGC TCCCTGCCCC CCGTCAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223693.1
CDS	1..3534
Translation	MAAAAGAAAAAAAEGEAPAEMGALVLEKEPRGATERVHGSLGDTSRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	XM_005223636.2
Organism	Bos taurus(cattle)
Definition	Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223636.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGGG TGTCCAATGA CACATCTTCG 
CTGGAGACAG AGCGCGGGTT TGAGGAGCTG CCCCTCTGCA GCTGCCGCAT GGAGGCCCCC 
AAGATAGACC GCATCAGCGA GAGAGCAGGG CACAAGTGCA TGGCCACAGA GAGCGTGGAC 
GGAGAGCTGT CCGGCTGCAA TGCTGCCATC CTCAAGCGGG AGACCATGAG ACCGTCCAGC 
CGTGTGGCCC TGATGGTGCT CTGTGAGACC CACCGTGCGC GCATGGTCAA ACATCACTGC 
TGCCCCGGCT GTGGCTACTT CTGTACGGCG GGCACCTTCC TGGAGTGTCA CCCCGACTTC 
CGTGTGGCCC ACCGCTTCCA CAAGGCCTGT GTGTCCCAGC TGAACGGGAT GGTCTTCTGT 
CCCCACTGTG GGGAGGACGC ATCTGAGGCC CAGGAGGTGA CCATCCCCCG GGGGGATGGG 
GTGACCCCAC CGGCTGGCAC TGCAGCACCC GCTCCCCCAC CCCTGGCCCA GGATGCCCCC 
GGGAGAGCGG ACACTTCCCA GCCCAGTGCC CGGATGCGGG GGCAGGGGGA GCCTCGGCGT 
CCACCCTGCG ACCCTCTGGC CGACACCATC GACAGCTCAG GGCCCTCCCT AACCCTGCCC 
AATGGGGGCT GCCTCTCAGC TGTAGGGCTG CCACCAGGGC CAGGCCGGGA GGCCCTGGAG 
AAGGCCCTGG TCATCCAGGA GTCAGAGAGG CGGAAAAAGC TCCGGTTCCA CCCTCGGCAG 
TTGTACCTGT CCGTAAAGCA AGGGGAGCTG CAGAAGGTGA TCCTGATGCT GTTGGACAAC 
CTGGACCCCA ACTTCCAGAG TGACCAGCAG AGCAAGCGCA CACCCCTGCA CGCGGCTGCC 
CAGAAGGGCT CTGTGGAGAT CTGCCATGTG CTGCTGCAGG CTGGAGCCAA CATCAATGCC 
GTGGACAAGC AGCAGCGGAC GCCGCTGATG GAGGCCGTGG TGAACAACCA TCTGGAGGTG 
GCTCGCTACA TGGTGCAGCG TGGGGGCTGT GTCTACAGCA AGGAGGAAGA CGGCTCCACC 
TGCCTCCACC ACGCCGCCAA AATTGGGAAC CTGGAGATGG TCAGCCTGCT GCTGAGCACG 
GGACAGGTGG ACGTCAATGC CCAGGACAGC GGCGGGTGGA CCCCCATCAT CTGGGCTGCA 
GAGCACAAGC ACATAGAAGT GATCCGCATG CTTCTGACAC GGGGCGCGGA CGTCACTCTC 
ACCGACAACG AGGAGAACAT CTGCCTGCAC TGGGCCTCCT TCACCGGCAG CGCCGCCATC 
GCCGAGGTCC TCCTGAACGC CCGCTGCGAC CTCCACGCTG TCAATTACCA CGGGGACACA 
CCCCTGCACA TCGCGGCCCG GGAGAGCTAC CACGACTGCG TGCTGTTGTT CCTGTCACGC 
GGGGCGAACC CTGAGCTGCG GAACAAGGAG GGGGACACGG CGTGGGACCT GACCCCCGAG 
CGGTCCGACG TGTGGTTTGC CCTCCAGCTC AACCGCAAGC TCCGGCTCGG GGTGGGGAAT 
CGGGCCATCC GCACGGAGAA GATCATCTGC CGGGACGTGG CTCGGGGCTA TGAGAACGTC 
CCCATCCCCT GTGTCAATGG TGTGGACGGG GAGCCCTGCC CCGAGGATTA CAAGTACATC 
TCAGAGAACT GCGAGACGTC CACCATGAAC ATTGACCGCA ACATTACCCA CCTGCAGCAC 
TGCACGTGTG TGGATGACTG CTCCAGCTCC AACTGCCTGT GTGGCCAGCT CAGCATCCGC 
TGTTGGTATG ACAAGGATGG GCGGCTGCTC CAGGAATTTA ACAAGATCGA GCCCCCGCTG 
ATTTTTGAGT GTAACCAGGC ATGTTCCTGC TGGAGAAACT GCAAGAACAG AGTAGTGCAG 
AGCGGCATCA AGGTGCGACT GCAGCTCTAC CGAACAGCCA AGATGGGCTG GGGGGTCCGT 
GCCCTGCAGA CCATTCCCCA GGGTACCTTC ATTTGCGAGT ATGTCGGAGA ACTGATCTCT 
GATGCCGAGG CTGATGTGAG AGAGGACGAT TCTTATCTCT TCGACTTGGA CAACAAGGAT 
GGAGAGGTGT ACTGCATCGA TGCCCGTTAC TACGGCAACA TCAGCCGCTT CATCAACCAC 
TTGTGTGACC CCAACATCAT CCCCGTCCGA GTCTTCATGC TGCACCAAGA CCTGAGATTT 
CCACGCATCG CCTTCTTCAG TTCCCGAGAC ATCCGGGCCG GGGAGGAGCT GGGGTTTGAC 
TATGGTGACC GCTTCTGGGA CATCAAAAGC AAGTACTTCA CCTGCCAGTG TGGCTCTGAG 
AAGTGCAAGC ACTCGGCTGA GGCCATTGCC CTGGAGCAGA GCCGCCTGGC CCGCCTGGAC 
CCACACCCTG AGCTGCTGCC TGAGCTCAGC TCCCTGCCCC CCGTCAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHMT2 ( XM_005223636.3 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005223693.1 Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X3, mRNA.

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CloneID	OBa32136
Clone ID Related Accession (Same CDS sequence)	XM_005223636.2 , XM_005223636.3
Accession Version	XM_005223636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase EHMT2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGGG TGTCCAATGA CACATCTTCG 
CTGGAGACAG AGCGCGGGTT TGAGGAGCTG CCCCTCTGCA GCTGCCGCAT GGAGGCCCCC 
AAGATAGACC GCATCAGCGA GAGAGCAGGG CACAAGTGCA TGGCCACAGA GAGCGTGGAC 
GGAGAGCTGT CCGGCTGCAA TGCTGCCATC CTCAAGCGGG AGACCATGAG ACCGTCCAGC 
CGTGTGGCCC TGATGGTGCT CTGTGAGACC CACCGTGCGC GCATGGTCAA ACATCACTGC 
TGCCCCGGCT GTGGCTACTT CTGTACGGCG GGCACCTTCC TGGAGTGTCA CCCCGACTTC 
CGTGTGGCCC ACCGCTTCCA CAAGGCCTGT GTGTCCCAGC TGAACGGGAT GGTCTTCTGT 
CCCCACTGTG GGGAGGACGC ATCTGAGGCC CAGGAGGTGA CCATCCCCCG GGGGGATGGG 
GTGACCCCAC CGGCTGGCAC TGCAGCACCC GCTCCCCCAC CCCTGGCCCA GGATGCCCCC 
GGGAGAGCGG ACACTTCCCA GCCCAGTGCC CGGATGCGGG GGCAGGGGGA GCCTCGGCGT 
CCACCCTGCG ACCCTCTGGC CGACACCATC GACAGCTCAG GGCCCTCCCT AACCCTGCCC 
AATGGGGGCT GCCTCTCAGC TGTAGGGCTG CCACCAGGGC CAGGCCGGGA GGCCCTGGAG 
AAGGCCCTGG TCATCCAGGA GTCAGAGAGG CGGAAAAAGC TCCGGTTCCA CCCTCGGCAG 
TTGTACCTGT CCGTAAAGCA AGGGGAGCTG CAGAAGGTGA TCCTGATGCT GTTGGACAAC 
CTGGACCCCA ACTTCCAGAG TGACCAGCAG AGCAAGCGCA CACCCCTGCA CGCGGCTGCC 
CAGAAGGGCT CTGTGGAGAT CTGCCATGTG CTGCTGCAGG CTGGAGCCAA CATCAATGCC 
GTGGACAAGC AGCAGCGGAC GCCGCTGATG GAGGCCGTGG TGAACAACCA TCTGGAGGTG 
GCTCGCTACA TGGTGCAGCG TGGGGGCTGT GTCTACAGCA AGGAGGAAGA CGGCTCCACC 
TGCCTCCACC ACGCCGCCAA AATTGGGAAC CTGGAGATGG TCAGCCTGCT GCTGAGCACG 
GGACAGGTGG ACGTCAATGC CCAGGACAGC GGCGGGTGGA CCCCCATCAT CTGGGCTGCA 
GAGCACAAGC ACATAGAAGT GATCCGCATG CTTCTGACAC GGGGCGCGGA CGTCACTCTC 
ACCGACAACG AGGAGAACAT CTGCCTGCAC TGGGCCTCCT TCACCGGCAG CGCCGCCATC 
GCCGAGGTCC TCCTGAACGC CCGCTGCGAC CTCCACGCTG TCAATTACCA CGGGGACACA 
CCCCTGCACA TCGCGGCCCG GGAGAGCTAC CACGACTGCG TGCTGTTGTT CCTGTCACGC 
GGGGCGAACC CTGAGCTGCG GAACAAGGAG GGGGACACGG CGTGGGACCT GACCCCCGAG 
CGGTCCGACG TGTGGTTTGC CCTCCAGCTC AACCGCAAGC TCCGGCTCGG GGTGGGGAAT 
CGGGCCATCC GCACGGAGAA GATCATCTGC CGGGACGTGG CTCGGGGCTA TGAGAACGTC 
CCCATCCCCT GTGTCAATGG TGTGGACGGG GAGCCCTGCC CCGAGGATTA CAAGTACATC 
TCAGAGAACT GCGAGACGTC CACCATGAAC ATTGACCGCA ACATTACCCA CCTGCAGCAC 
TGCACGTGTG TGGATGACTG CTCCAGCTCC AACTGCCTGT GTGGCCAGCT CAGCATCCGC 
TGTTGGTATG ACAAGGATGG GCGGCTGCTC CAGGAATTTA ACAAGATCGA GCCCCCGCTG 
ATTTTTGAGT GTAACCAGGC ATGTTCCTGC TGGAGAAACT GCAAGAACAG AGTAGTGCAG 
AGCGGCATCA AGGTGCGACT GCAGCTCTAC CGAACAGCCA AGATGGGCTG GGGGGTCCGT 
GCCCTGCAGA CCATTCCCCA GGGTACCTTC ATTTGCGAGT ATGTCGGAGA ACTGATCTCT 
GATGCCGAGG CTGATGTGAG AGAGGACGAT TCTTATCTCT TCGACTTGGA CAACAAGGAT 
GGAGAGGTGT ACTGCATCGA TGCCCGTTAC TACGGCAACA TCAGCCGCTT CATCAACCAC 
TTGTGTGACC CCAACATCAT CCCCGTCCGA GTCTTCATGC TGCACCAAGA CCTGAGATTT 
CCACGCATCG CCTTCTTCAG TTCCCGAGAC ATCCGGGCCG GGGAGGAGCT GGGGTTTGAC 
TATGGTGACC GCTTCTGGGA CATCAAAAGC AAGTACTTCA CCTGCCAGTG TGGCTCTGAG 
AAGTGCAAGC ACTCGGCTGA GGCCATTGCC CTGGAGCAGA GCCGCCTGGC CCGCCTGGAC 
CCACACCCTG AGCTGCTGCC TGAGCTCAGC TCCCTGCCCC CCGTCAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223693.1
CDS	11..3544
Translation	MAAAAGAAAAAAAEGEAPAEMGALVLEKEPRGATERVHGSLGDTSRSEETLPKANPDSLETAGPSSPASVTVTVGDEGADTPVGATPLIGDEPENLEGDGDLHGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAPAAGPEPPPATASPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEVQHFRMSDDVHSLGKVTSDVAKRRKLNSGGGLSEELASTRSSGEVTLEKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLAQDAPGRADTSQPSARMRGQGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRAGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELSSLPPVNP

Target ORF information:

RefSeq Version	XM_005223636.3
Organism	Bos taurus(cattle)
Definition	Bos taurus euchromatic histone lysine methyltransferase 2 (EHMT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223636.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCAGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGGTGCTGGA GAAGGAGCCC AGAGGAGCCA CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCGTAG TGAGGAGACC CTGCCCAAGG CCAACCCCGA TTCCCTGGAG 
ACTGCTGGCC CCTCATCCCC AGCCTCTGTC ACAGTCACTG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTAG GGGCCACACC ACTCATTGGG GATGAACCCG AGAATCTCGA GGGAGATGGG 
GACCTCCATG GGGGTCGCAT CCTGCTGGGC CATGCCACAA AGTCGTTCCC GTCTTCCCCC 
AGCAAGGGGG GTGCCTGTCC CAGCCGAGCC AAGATGTCAA TGACAGGGGC CGGAAAATCA 
CCCCCATCAG TTCAGAGTTT GGCTATGAGG CTGCTAAGTA TGCCGGGCGC CCAAGGGGCG 
CCAGCAGCAG GGCCTGAACC CCCTCCGGCC ACAGCCAGCC CAGAGGGGCA GCCCAAGGTT 
CATCGAGCCA GGAAAACCAT GTCCAAACCA GGAAATGGAC AGCCCCCAGT CCCTGAGAAG 
CGGCCCCCTG AAGTGCAGCA TTTCCGCATG AGTGATGACG TGCACTCGCT GGGGAAGGTG 
ACCTCAGATG TGGCCAAAAG GAGGAAGCTG AACTCAGGAG GTGGCTTGTC AGAAGAGTTG 
GCTTCTACGC GGAGTTCGGG AGAAGTGACC CTGGAGAAGG GGGACCCCGG GTCCCTGGAG 
GAGTGGGAAA CGGTGGTAGG GGATGACTTC AGCCTCTACT ATGATTCCTA CTCTGTGGAT 
GAGCGGGTGG ACTCTGACAG CAAGTCTGAG GTTGAAGCTC TGGCTGAACA ACTGAGTGAG 
GAAGAGGAAG AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AGGAGGAGGA GGAGGAGGAA 
GAAGAAGAGG AAGATGAAGA GTCTGGCAAT CAGTCTGACA GGAGTGGTTC CAGCGGCCGC 
CGCAAAGCCA AGAAGAAATG GCGGAAGGAC AGCCCATGGG TGAAGCCATC ACGGAAACGG 
CGGAAACGAG AGCCTCCGAG GGCCAAGGAG CCACGAGGGG TGTCCAATGA CACATCTTCG 
CTGGAGACAG AGCGCGGGTT TGAGGAGCTG CCCCTCTGCA GCTGCCGCAT GGAGGCCCCC 
AAGATAGACC GCATCAGCGA GAGAGCAGGG CACAAGTGCA TGGCCACAGA GAGCGTGGAC 
GGAGAGCTGT CCGGCTGCAA TGCTGCCATC CTCAAGCGGG AGACCATGAG ACCGTCCAGC 
CGTGTGGCCC TGATGGTGCT CTGTGAGACC CACCGTGCGC GCATGGTCAA ACATCACTGC 
TGCCCCGGCT GTGGCTACTT CTGTACGGCG GGCACCTTCC TGGAGTGTCA CCCCGACTTC 
CGTGTGGCCC ACCGCTTCCA CAAGGCCTGT GTGTCCCAGC TGAACGGGAT GGTCTTCTGT 
CCCCACTGTG GGGAGGACGC ATCTGAGGCC CAGGAGGTGA CCATCCCCCG GGGGGATGGG 
GTGACCCCAC CGGCTGGCAC TGCAGCACCC GCTCCCCCAC CCCTGGCCCA GGATGCCCCC 
GGGAGAGCGG ACACTTCCCA GCCCAGTGCC CGGATGCGGG GGCAGGGGGA GCCTCGGCGT 
CCACCCTGCG ACCCTCTGGC CGACACCATC GACAGCTCAG GGCCCTCCCT AACCCTGCCC 
AATGGGGGCT GCCTCTCAGC TGTAGGGCTG CCACCAGGGC CAGGCCGGGA GGCCCTGGAG 
AAGGCCCTGG TCATCCAGGA GTCAGAGAGG CGGAAAAAGC TCCGGTTCCA CCCTCGGCAG 
TTGTACCTGT CCGTAAAGCA AGGGGAGCTG CAGAAGGTGA TCCTGATGCT GTTGGACAAC 
CTGGACCCCA ACTTCCAGAG TGACCAGCAG AGCAAGCGCA CACCCCTGCA CGCGGCTGCC 
CAGAAGGGCT CTGTGGAGAT CTGCCATGTG CTGCTGCAGG CTGGAGCCAA CATCAATGCC 
GTGGACAAGC AGCAGCGGAC GCCGCTGATG GAGGCCGTGG TGAACAACCA TCTGGAGGTG 
GCTCGCTACA TGGTGCAGCG TGGGGGCTGT GTCTACAGCA AGGAGGAAGA CGGCTCCACC 
TGCCTCCACC ACGCCGCCAA AATTGGGAAC CTGGAGATGG TCAGCCTGCT GCTGAGCACG 
GGACAGGTGG ACGTCAATGC CCAGGACAGC GGCGGGTGGA CCCCCATCAT CTGGGCTGCA 
GAGCACAAGC ACATAGAAGT GATCCGCATG CTTCTGACAC GGGGCGCGGA CGTCACTCTC 
ACCGACAACG AGGAGAACAT CTGCCTGCAC TGGGCCTCCT TCACCGGCAG CGCCGCCATC 
GCCGAGGTCC TCCTGAACGC CCGCTGCGAC CTCCACGCTG TCAATTACCA CGGGGACACA 
CCCCTGCACA TCGCGGCCCG GGAGAGCTAC CACGACTGCG TGCTGTTGTT CCTGTCACGC 
GGGGCGAACC CTGAGCTGCG GAACAAGGAG GGGGACACGG CGTGGGACCT GACCCCCGAG 
CGGTCCGACG TGTGGTTTGC CCTCCAGCTC AACCGCAAGC TCCGGCTCGG GGTGGGGAAT 
CGGGCCATCC GCACGGAGAA GATCATCTGC CGGGACGTGG CTCGGGGCTA TGAGAACGTC 
CCCATCCCCT GTGTCAATGG TGTGGACGGG GAGCCCTGCC CCGAGGATTA CAAGTACATC 
TCAGAGAACT GCGAGACGTC CACCATGAAC ATTGACCGCA ACATTACCCA CCTGCAGCAC 
TGCACGTGTG TGGATGACTG CTCCAGCTCC AACTGCCTGT GTGGCCAGCT CAGCATCCGC 
TGTTGGTATG ACAAGGATGG GCGGCTGCTC CAGGAATTTA ACAAGATCGA GCCCCCGCTG 
ATTTTTGAGT GTAACCAGGC ATGTTCCTGC TGGAGAAACT GCAAGAACAG AGTAGTGCAG 
AGCGGCATCA AGGTGCGACT GCAGCTCTAC CGAACAGCCA AGATGGGCTG GGGGGTCCGT 
GCCCTGCAGA CCATTCCCCA GGGTACCTTC ATTTGCGAGT ATGTCGGAGA ACTGATCTCT 
GATGCCGAGG CTGATGTGAG AGAGGACGAT TCTTATCTCT TCGACTTGGA CAACAAGGAT 
GGAGAGGTGT ACTGCATCGA TGCCCGTTAC TACGGCAACA TCAGCCGCTT CATCAACCAC 
TTGTGTGACC CCAACATCAT CCCCGTCCGA GTCTTCATGC TGCACCAAGA CCTGAGATTT 
CCACGCATCG CCTTCTTCAG TTCCCGAGAC ATCCGGGCCG GGGAGGAGCT GGGGTTTGAC 
TATGGTGACC GCTTCTGGGA CATCAAAAGC AAGTACTTCA CCTGCCAGTG TGGCTCTGAG 
AAGTGCAAGC ACTCGGCTGA GGCCATTGCC CTGGAGCAGA GCCGCCTGGC CCGCCTGGAC 
CCACACCCTG AGCTGCTGCC TGAGCTCAGC TCCCTGCCCC CCGTCAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of EHMT2 Induces a Robust Antiviral Response Against Foot-and-Mouth Disease and Vesicular Stomatitis Virus Infections in Bovine Cells.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research36(1)37-47(2016 Jan)
Singh N,Ram?rez-Carvajal L,de Los Santos T,Golding MC,Long CR

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

YAC/BAC contig spanning the MHC class III region of cattle.
Cytogenetic and genome research115(1)45-50(2006)
Gelhaus A,Hess M,F?rster B,Goldammer T,Schwerin M,Horstmann RD

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EHMT2 cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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