EIF2AK4 cDNA ORF clone, Bos taurus(cattle)

The following EIF2AK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2AK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06472 XM_002690785.5
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa06472 XM_001787661.6
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa06473 XM_005211622.4
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa06473 XM_005192397.3
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48749 XM_010809210.3
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48749 XM_010822894.2
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48750 XM_010822899.2
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48750 XM_010809211.3
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48751 XM_024997963.1
Latest version!
Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OBa06472 XM_002690785.4 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48749 XM_010809210.2 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa48750 XM_010809211.2 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa06473 XM_005211622.3 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa06472
    Clone ID Related Accession (Same CDS sequence) XM_002690785.4 , XM_001787661.6 , XM_002690785.5
    Accession Version XM_002690785.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690831.1
    CDS1..4950
    Translation

    Target ORF information:

    RefSeq Version XM_002690785.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06472
    Clone ID Related Accession (Same CDS sequence) XM_002690785.4 , XM_001787661.6 , XM_002690785.5
    Accession Version XM_001787661.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001787661.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001787713.2
    CDS1..4950
    Translation

    Target ORF information:

    RefSeq Version XM_001787661.6
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001787661.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06472
    Clone ID Related Accession (Same CDS sequence) XM_002690785.4 , XM_001787661.6 , XM_002690785.5
    Accession Version XM_002690785.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690785.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690831.1
    CDS57..5006
    Translation

    Target ORF information:

    RefSeq Version XM_002690785.5
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690785.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA
    GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC
    CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT
    GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC
    AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA
    GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA
    AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC
    TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC
    AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC
    ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT
    GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC
    AGAGATGACT ACTACAGAAT CTTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06473
    Clone ID Related Accession (Same CDS sequence) XM_005192397.3 , XM_005211622.4 , XM_005211622.3
    Accession Version XM_005192397.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192454.1
    CDS71..4912
    Translation

    Target ORF information:

    RefSeq Version XM_005192397.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192397.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06473
    Clone ID Related Accession (Same CDS sequence) XM_005192397.3 , XM_005211622.4 , XM_005211622.3
    Accession Version XM_005211622.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase GCN2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211679.1
    CDS73..4914
    Translation

    Target ORF information:

    RefSeq Version XM_005211622.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211622.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06473
    Clone ID Related Accession (Same CDS sequence) XM_005192397.3 , XM_005211622.4 , XM_005211622.3
    Accession Version XM_005211622.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211679.1
    CDS71..4912
    Translation

    Target ORF information:

    RefSeq Version XM_005211622.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211622.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA
    GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT
    AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT
    GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT
    GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT
    TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG
    GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC
    CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT
    GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA
    CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC
    AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC
    CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48749
    Clone ID Related Accession (Same CDS sequence) XM_010809210.3 , XM_010809210.2 , XM_010822894.2
    Accession Version XM_010809210.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807512.1
    CDS73..5055
    Translation

    Target ORF information:

    RefSeq Version XM_010809210.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809210.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48749
    Clone ID Related Accession (Same CDS sequence) XM_010809210.3 , XM_010809210.2 , XM_010822894.2
    Accession Version XM_010809210.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807512.1
    CDS71..5053
    Translation

    Target ORF information:

    RefSeq Version XM_010809210.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809210.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48749
    Clone ID Related Accession (Same CDS sequence) XM_010809210.3 , XM_010809210.2 , XM_010822894.2
    Accession Version XM_010822894.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821196.1
    CDS71..5053
    Translation

    Target ORF information:

    RefSeq Version XM_010822894.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA
    CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG
    GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG
    TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA
    AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48750
    Clone ID Related Accession (Same CDS sequence) XM_010809211.3 , XM_010822899.2 , XM_010809211.2
    Accession Version XM_010809211.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase GCN2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807513.1
    CDS73..4947
    Translation

    Target ORF information:

    RefSeq Version XM_010809211.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809211.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48750
    Clone ID Related Accession (Same CDS sequence) XM_010809211.3 , XM_010822899.2 , XM_010809211.2
    Accession Version XM_010822899.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821201.1
    CDS71..4945
    Translation

    Target ORF information:

    RefSeq Version XM_010822899.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48750
    Clone ID Related Accession (Same CDS sequence) XM_010809211.3 , XM_010822899.2 , XM_010809211.2
    Accession Version XM_010809211.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807513.1
    CDS71..4945
    Translation

    Target ORF information:

    RefSeq Version XM_010809211.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809211.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT
    TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC
    CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC
    CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT
    CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG
    TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC
    GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT
    AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC
    CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC
    ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA
    CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA
    CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG
    CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC
    ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC
    AGAATCTTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48751
    Clone ID Related Accession (Same CDS sequence) XM_024997963.1
    Accession Version XM_024997963.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase GCN2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGGTTTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853731.1
    CDS73..4617
    Translation

    Target ORF information:

    RefSeq Version XM_024997963.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997963.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
    CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC
    CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA
    CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT
    GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG
    GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC
    CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG
    CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG
    AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT
    TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC
    AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT
    TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG
    CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG
    GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA
    ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC
    TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG
    CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT
    CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC
    GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG
    ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG
    CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT
    ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT
    CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC
    TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT
    CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT
    GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG
    AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT
    TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC
    ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG
    CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA
    GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC
    GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC
    AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG
    CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT
    GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC
    AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG
    ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC
    ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG
    AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT
    GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA
    CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG
    ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC
    AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC
    ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT
    AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG
    CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG
    CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC
    AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC
    CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA
    GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT
    GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA
    GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT
    CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA
    CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA
    TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC
    ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG
    AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC
    AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG
    GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT
    ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG
    AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA
    CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG
    GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG
    GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA
    GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG
    ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG
    GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG
    TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT
    GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC
    TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG
    GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA
    CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGGTTTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.