EIF2AK4 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_002690785.4	XP_002690831.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
XM_001787661.6	XP_001787713.2	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
XM_002690785.5	XP_002690831.1	eIF-2-alpha kinase GCN2 isoform X2
XM_010809211.3	XP_010807513.1	eIF-2-alpha kinase GCN2 isoform X3
XM_010822899.2	XP_010821201.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
XM_005192397.3	XP_005192454.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
XM_010809210.3	XP_010807512.1	eIF-2-alpha kinase GCN2 isoform X1
XM_005211622.4	XP_005211679.1	eIF-2-alpha kinase GCN2 isoform X4
XM_024997963.1	XP_024853731.1	eIF-2-alpha kinase GCN2 isoform X5
XM_010809210.2	XP_010807512.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
XM_010809211.2	XP_010807513.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
XM_005211622.3	XP_005211679.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
XM_010822894.2	XP_010821196.1	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa06472	XM_002690785.5 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa06472	XM_001787661.6 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa06473	XM_005211622.4 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa06473	XM_005192397.3 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48749	XM_010809210.3 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48749	XM_010822894.2 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48750	XM_010822899.2 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48750	XM_010809211.3 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48751	XM_024997963.1 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OBa06472	XM_002690785.4	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48749	XM_010809210.2	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa48750	XM_010809211.2	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa06473	XM_005211622.3	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

EIF2AK4 ( XM_002690785.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690831.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa06472
Clone ID Related Accession (Same CDS sequence)	XM_002690785.4 , XM_001787661.6 , XM_002690785.5
Accession Version	XM_002690785.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690831.1
CDS	1..4950
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_002690785.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690785.4

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_001787661.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001787713.2 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

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CloneID	OBa06472
Clone ID Related Accession (Same CDS sequence)	XM_002690785.4 , XM_001787661.6 , XM_002690785.5
Accession Version	XM_001787661.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001787661.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001787713.2
CDS	1..4950
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_001787661.6
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001787661.6

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_002690785.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690831.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa06472
Clone ID Related Accession (Same CDS sequence)	XM_002690785.4 , XM_001787661.6 , XM_002690785.5
Accession Version	XM_002690785.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690785.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690831.1
CDS	57..5006
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_002690785.5
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690785.5

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CACGTCAAGG TAAAGTCTTT TGAGAAGGAC AGGCAAACAG AGAAACGTGT GCCGGAGTCT 
GATCTCGTGG ACCACGTTGT ACAGAAACTG AGGACTAAAG TCAGTGATGA GAGGACTACC 
AGAGAAGCTT CCGATAATCT TACAGTACAA AATCTGAAGG GATCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACGGTT GTTCCCATTG TGAGTGTGGT CGCCCCAGAA 
AAGCTGTCAG CCAGCACCCG ACGGCGGTAT GAGACTCAGG TACAAACCCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTTTGGCTGT GGATCTACCC 
AAAGAAACAA TCTTACAGTT CCTGTCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAGATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTCTTCCT GTACAGCTAC 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_005192397.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192454.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

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CloneID	OBa06473
Clone ID Related Accession (Same CDS sequence)	XM_005192397.3 , XM_005211622.4 , XM_005211622.3
Accession Version	XM_005192397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192454.1
CDS	71..4912
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_005192397.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192397.3

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_005211622.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211679.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

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CloneID	OBa06473
Clone ID Related Accession (Same CDS sequence)	XM_005192397.3 , XM_005211622.4 , XM_005211622.3
Accession Version	XM_005211622.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211679.1
CDS	73..4914
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_005211622.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211622.4

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_005211622.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211679.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

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CloneID	OBa06473
Clone ID Related Accession (Same CDS sequence)	XM_005192397.3 , XM_005211622.4 , XM_005211622.3
Accession Version	XM_005211622.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211679.1
CDS	71..4912
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_005211622.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211622.3

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGTCCCAA 
GAGGAATTAC AAGAGTACTG CAGACATCAT GAAATCACCT ATGTGGCCCT TGTCTCGGAT 
AAAGAAGGAA GCCACGTCAA GGTAAAGTCT TTTGAGAAGG ACAGGCAAAC AGAGAAACGT 
GTGCCGGAGT CTGATCTCGT GGACCACGTT GTACAGAAAC TGAGGACTAA AGTCAGTGAT 
GAGAGGACTA CCAGAGAAGC TTCCGATAAT CTTACAGTAC AAAATCTGAA GGGATCATTT 
TCTAATGCTT CAGGTTTGTT TGAAATCCAT GGAGCAACGG TTGTTCCCAT TGTGAGTGTG 
GTCGCCCCAG AAAAGCTGTC AGCCAGCACC CGACGGCGGT ATGAGACTCA GGTACAAACC 
CGACTTCAGA CCTCCCTTGC CAACTTACAT CAGAAAAGCA GTGAAATTGA AATTTTGGCT 
GTGGATCTAC CCAAAGAAAC AATCTTACAG TTCCTGTCAT TAGAGTGGGA TGCTGATGAA 
CAGGCATTTA ACACAACTGT GAAGCAGCTG CTGTCACGCC TGCCAAAGCA AAGATACCTC 
AAATTAGTCT GTGATGAGAT TTATAACATC AAAGTAGAAA AAAAGGTGTC TGTGCTCTTC 
CTGTACAGCT ACAGAGATGA CTACTACAGA ATCTTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010809210.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807512.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

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CloneID	OBa48749
Clone ID Related Accession (Same CDS sequence)	XM_010809210.3 , XM_010809210.2 , XM_010822894.2
Accession Version	XM_010809210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807512.1
CDS	73..5055
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010809210.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809210.3

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010809210.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807512.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48749
Clone ID Related Accession (Same CDS sequence)	XM_010809210.3 , XM_010809210.2 , XM_010822894.2
Accession Version	XM_010809210.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807512.1
CDS	71..5053
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010809210.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809210.2

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010822894.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821196.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

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CloneID	OBa48749
Clone ID Related Accession (Same CDS sequence)	XM_010809210.3 , XM_010809210.2 , XM_010822894.2
Accession Version	XM_010822894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821196.1
CDS	71..5053
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010822894.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822894.2

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGAGAAG CTTCCGATAA TCTTACAGTA 
CAAAATCTGA AGGGATCATT TTCTAATGCT TCAGGTTTGT TTGAAATCCA TGGAGCAACG 
GTTGTTCCCA TTGTGAGTGT GGTCGCCCCA GAAAAGCTGT CAGCCAGCAC CCGACGGCGG 
TATGAGACTC AGGTACAAAC CCGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC 
AGTGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTCCTGTCA 
TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC 
CTGCCAAAGC AAAGATACCT CAAATTAGTC TGTGATGAGA TTTATAACAT CAAAGTAGAA 
AAAAAGGTGT CTGTGCTCTT CCTGTACAGC TACAGAGATG ACTACTACAG AATCTTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010809211.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807513.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

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CloneID	OBa48750
Clone ID Related Accession (Same CDS sequence)	XM_010809211.3 , XM_010822899.2 , XM_010809211.2
Accession Version	XM_010809211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807513.1
CDS	73..4947
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010809211.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809211.3

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010822899.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821201.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

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CloneID	OBa48750
Clone ID Related Accession (Same CDS sequence)	XM_010809211.3 , XM_010822899.2 , XM_010809211.2
Accession Version	XM_010822899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821201.1
CDS	71..4945
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010822899.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822899.2

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_010809211.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807513.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

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CloneID	OBa48750
Clone ID Related Accession (Same CDS sequence)	XM_010809211.3 , XM_010822899.2 , XM_010809211.2
Accession Version	XM_010809211.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807513.1
CDS	71..4945
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTREASDNLTVQNLKGSFSNASGLFEIHGATVVPIVSVVAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_010809211.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809211.2

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGGTTAC AGTAAGCTCT 
TGTGACCTCC TGGTTGTAAG TGTTGGTCAG ATGTCCATGT CCAGGGCCAT CAACCTAACC 
CAGAAACTCT GGTCAGCTGG AATCACAGCA GAAATCATGT ATGACTGGTC ACAGCTGTTC 
CAAGTGCTTT GTGTCTATTT GTTCCAGTCC CAAGAGGAAT TACAAGAGTA CTGCAGACAT 
CATGAAATCA CCTATGTGGC CCTTGTCTCG GATAAAGAAG GAAGCCACGT CAAGGTAAAG 
TCTTTTGAGA AGGACAGGCA AACAGAGAAA CGTGTGCCGG AGTCTGATCT CGTGGACCAC 
GTTGTACAGA AACTGAGGAC TAAAGTCAGT GATGAGAGGA CTACCAGAGA AGCTTCCGAT 
AATCTTACAG TACAAAATCT GAAGGGATCA TTTTCTAATG CTTCAGGTTT GTTTGAAATC 
CATGGAGCAA CGGTTGTTCC CATTGTGAGT GTGGTCGCCC CAGAAAAGCT GTCAGCCAGC 
ACCCGACGGC GGTATGAGAC TCAGGTACAA ACCCGACTTC AGACCTCCCT TGCCAACTTA 
CATCAGAAAA GCAGTGAAAT TGAAATTTTG GCTGTGGATC TACCCAAAGA AACAATCTTA 
CAGTTCCTGT CATTAGAGTG GGATGCTGAT GAACAGGCAT TTAACACAAC TGTGAAGCAG 
CTGCTGTCAC GCCTGCCAAA GCAAAGATAC CTCAAATTAG TCTGTGATGA GATTTATAAC 
ATCAAAGTAG AAAAAAAGGT GTCTGTGCTC TTCCTGTACA GCTACAGAGA TGACTACTAC 
AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_024997963.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853731.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.

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CloneID	OBa48751
Clone ID Related Accession (Same CDS sequence)	XM_024997963.1
Accession Version	XM_024997963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eIF-2-alpha kinase GCN2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGGTTTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853731.1
CDS	73..4617
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDARGRVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEEVAKKHCGEVMIFELAYYVQSFLCEHNKPPPKSFHEEMLERRAQEEQRRQLEAKRKEEEEQREILHEIQKRKEEIKEEKKRKEMAKQERLEIAILSNQDHTSRKDPGGHRTAAILHGGSPDFVGNGKHRTNSSGRSKRERQYSMCNSEDSPGSCEILYFSVGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGSFVLLHEWVLQWHKKMGPFLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNLKEQDDSIVVDILVEHTNGISLAAHLGHSGPIPVHQLRKYAAQLLSGLDYLHSNSVVHKVLSALNVLVDAEGTIKITDYSISKRLADICKEDVFEQTRVRFSDSALPYKTGKKGDIWRLGLLLLSLSQGQECEEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQSKIPLLEQSPEDSGGQDYVETVIPSNQLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPACPATPPPNSGPQAQDGQALAQPAGDSDPDGPGSVEAAAPPPILSSSVEWSTSGERSASTRFPAAGPDSSSDEDDDEEQGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNGKNSCHESEPPATTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFAADGKQDDTSGDHLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHTKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLANVDGKAYRTMMGQIFAQRISPAIDYTYDSDILKGSFSIRTAKIQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSSSLCRLYKFIEQKGELQDLTPTINSLIKQKTGVAQLVKYGLKDLEEVVGLLKKFGIKLQVLINLGLVYKVQQHSGIIFQFVAFIRRRQRAISEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKITTAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWSAGITAEIMYDWSQLFQVLCVYLFQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKDRQTEKRVPESDLVDHVVQKLRTKVSDERTTRFV

Target ORF information:

RefSeq Version	XM_024997963.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997963.1

ORF Insert Sequence:

ATGGCCGGGG GCCGCGGGGC CCCCGGACGT GGCCGGGACG AGCCACCGGA GAGCTACCCG 
CAACGACAAG ACCACGAGCT GCAGGCCCTG GAGGCCATCT ATGGCGCCGA CTTCCAGGAC 
CTGCGGCCGG ACGCGCGCGG ACGGGTCAAA GAGCCTCCTG AAATCAATTT AGTTCTGTAC 
CCTCAAGGCC TGACTGGTGA AGAAGTGTAC GTGAAAGTGG ATTTGAGAGT GAAATGCCCA 
CCCACCTATC CAGATGTAGT CCCTGAAATA GAATTAAAGA ATGCCAAAGG TCTGTCAAAT 
GAAAGTGTTA ATTTGTTAAA GTCCCGCCTA GAAGAAGTGG CCAAAAAACA CTGTGGTGAG 
GTGATGATAT TTGAACTGGC TTACTATGTG CAGTCATTTC TCTGCGAGCA CAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGAG CTCAGGAGGA GCAGCGGAGG 
CAGTTAGAGG CCAAGCGGAA AGAGGAAGAG GAGCAACGTG AAATCCTTCA TGAGATTCAG 
AAAAGGAAAG AAGAGATAAA AGAGGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTAGAAATAG CTATTTTGTC AAATCAAGAT CATACCTCTA GAAAAGACCC AGGAGGACAC 
AGAACAGCTG CCATTCTACA TGGAGGCTCT CCTGACTTCG TAGGAAATGG TAAACATCGG 
ACCAACTCCT CTGGAAGGTC TAAGCGAGAA CGTCAGTATT CTATGTGTAA TAGCGAAGAT 
TCTCCTGGCT CTTGTGAAAT CCTGTATTTC TCTGTGGGTA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA CAATGCTTTG 
GAAACAGCTA CTGGCAGCTT TGTCTTGTTG CATGAGTGGG TCCTTCAATG GCACAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGGGCAG AAACAGAATT CAGCTCACTG GTGAAACTGA GCCATCCAAA CATAGTACGC 
TACTTTGCGA TGAACCTCAA AGAGCAAGAC GACTCCATCG TGGTGGATAT TTTAGTGGAG 
CACACTAATG GGATCTCCCT CGCAGCCCAC CTGGGCCACT CAGGCCCCAT CCCGGTACAT 
CAGCTTCGCA AGTATGCAGC TCAGCTCTTG TCAGGCCTTG ATTACTTGCA CAGCAATTCC 
GTGGTGCACA AGGTTCTGAG CGCATTGAAT GTCCTGGTGG ATGCAGAGGG CACCATCAAG 
ATTACGGACT ACAGCATTTC TAAGCGCCTG GCAGACATTT GCAAGGAGGA CGTGTTTGAG 
CAGACCCGGG TTCGTTTCAG TGACAGCGCC CTACCTTATA AAACCGGGAA GAAAGGGGAT 
ATTTGGCGTC TCGGCCTTCT GCTGCTCTCC CTCAGCCAAG GACAAGAGTG TGAAGAGTAT 
CCCGTGACCA TCCCTAGTGA CTTACCAGCT GATTTTCAAG ATTTTCTAAA GAAGTGTGTC 
TGCTTAGATG ACAAGGAAAG ATGGAGTCCT CAGCAGTTGT TAAAACACAG CTTTATAAAT 
CCTCAGTCTA AAATACCTTT ACTGGAACAA AGTCCCGAAG ATTCTGGAGG ACAAGATTAT 
GTGGAGACTG TCATTCCTAG CAACCAGCTA CCCAGTGCTG CATTCTTCAG TGAGACCCAG 
AGACAGTTTT CCCGCTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCT 
TTTGGAGCCG TCATCAAGGT GCAGAACAAG CTGGACGGCT GCTGCTATGC GGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CAGGCACTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 
TCGCGCCTCC ACCATGAGAA CATCGTGCGT TACTACAATG CCTGGATCGA GAGACATGAG 
CGGCCGGCGT GCCCGGCGAC TCCACCCCCG AACTCGGGGC CCCAGGCCCA GGACGGGCAA 
GCCTTAGCCC AGCCAGCGGG CGACAGCGAC CCGGACGGCC CGGGCAGTGT GGAGGCGGCC 
GCGCCGCCGC CCATCCTCAG CAGCTCGGTG GAATGGAGCA CGTCAGGCGA GCGCTCGGCC 
AGCACCCGCT TCCCCGCCGC CGGCCCAGAC TCCAGCAGCG ACGAGGACGA CGACGAGGAG 
CAGGGCGTCT TCTCACAGTC CTTCCTACCT GCTTCAGATT CTGACAGTGA TATCATCTTT 
GACAATGAAG ATGAGAACAG TAAAAGCCAG AATCAGGATG AAGATTGCAA TGGAAAGAAC 
AGTTGTCATG AAAGTGAGCC ACCAGCAACC ACCGAGGCTG TGCACTACCT CTACATCCAG 
ATGGAGTACT GTGAGAAGAG CACTTTACGA GACACCATTG ACCAGGGACT GTATCGAGAC 
ACCGTCAGGC TCTGGAGGCT TTTCCGAGAG ATTCTGGATG GATTGGCTTA TATCCATGAG 
AAAGGAATGA TTCACCGAGA TTTGAAGCCT GTCAACATTT TTTTGGATTC TGATGACCAT 
GTCAAAATAG GTGATTTTGG TTTGGCCACC GACCATCTAG CCTTTGCTGC TGACGGCAAA 
CAAGATGACA CATCAGGAGA TCACTTGATT AAATCAGACC CTTCAGGTCA TTTGACTGGG 
ATGGTTGGCA CTGCTTTATA TGTAAGCCCT GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGG ATTATCTTCT TTGAGATGTC CTATCATCCC 
ATGGTCACAG CCTCAGAAAG AATCTTTGTT CTCAACCAGC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTCGA TGATGGGGAG CATACAAAGC AGAAATCTGT CATCTCCTGG 
CTGTTGAACC ACGATCCGGC AAAGCGGCCC ACGGCCACAG AACTGCTCAA GAGCGAGCTG 
CTGCCCCCGC CCCAGATGGA GGAATCGGAG CTGCACGAGG TGCTGCACCA CACCCTGGCC 
AACGTGGACG GGAAGGCCTA CCGCACCATG ATGGGCCAGA TCTTCGCCCA GCGCATCTCC 
CCTGCCATCG ACTACACCTA CGACAGTGAC ATACTGAAGG GCAGCTTCTC CATCCGCACA 
GCCAAGATAC AGCAGCACGT GTGTGAAACC ATCATCCGCA TCTTCAAAAG ACACGGAGCT 
GTCCAGTTGT GTACCCCACT GCTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCTTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TGCCTTTCGA CCTGCGGGTT 
CCTTTTGCAA GATATGTGGC GAGAAATAAT ATATTGAATT TAAAGCGATA TTGCATAGAA 
CGTGTGTTCA GACCTCGAAA ATTAGACCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TTACCTCTAC TACCAACAGC TCGCTGCCCA CCGCTGAAAT CATCTACACC 
ATCTACGAAA TCATCCAGGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTACTTG 
AACCATACCA TGTTACTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGCCAAGTCT ATGTTATTCT GTATGATGCT GTGACAGAAA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT ATCCTTGTCG TCAAGTAGTC TGTGTCGACT CTACAAATTT 
ATTGAGCAGA AGGGAGAGCT GCAAGATCTA ACCCCAACCA TAAATTCATT AATAAAACAG 
AAAACAGGTG TTGCCCAGTT GGTGAAGTAC GGCTTAAAAG ATCTGGAGGA GGTCGTTGGA 
CTGTTGAAGA AATTTGGCAT AAAGTTACAG GTCCTGATCA ATCTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAGCGGAAT CATCTTCCAG TTTGTGGCAT TCATCAGACG GAGGCAAAGG 
GCTATATCTG AAATCCTCGC AGCTGGTGGT AGATACGACC TGCTGATTCC CCAGTTTAGA 
GGACCACAGG CTCTGGGGCC AGTTCCTACT GCCATCGGGG TCAGCATAGC CATAGACAAG 
ATAACCACTG CTGTCCTCAA CATGGAAGAG CCTGTTACAG TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGTCAGAT GTCCATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
TCAGCTGGAA TCACAGCAGA AATCATGTAT GACTGGTCAC AGCTGTTCCA AGTGCTTTGT 
GTCTATTTGT TCCAGTCCCA AGAGGAATTA CAAGAGTACT GCAGACATCA TGAAATCACC 
TATGTGGCCC TTGTCTCGGA TAAAGAAGGA AGCCACGTCA AGGTAAAGTC TTTTGAGAAG 
GACAGGCAAA CAGAGAAACG TGTGCCGGAG TCTGATCTCG TGGACCACGT TGTACAGAAA 
CTGAGGACTA AAGTCAGTGA TGAGAGGACT ACCAGGTTTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EIF2AK4
Entrez Gene ID	513829
Full Name	eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms	Gcn2-like
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 4 Names eukaryotic translation initiation factor 2-alpha kinase 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EIF2AK4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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