AKAP12 cDNA ORF clone, Bos taurus(cattle)

The following AKAP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa48727 XM_010808687.2
Latest version!
Bos taurus A-kinase anchoring protein 12 (AKAP12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa48727 XM_015464860.1
Latest version!
Bos taurus A-kinase anchoring protein 12 (AKAP12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa238112 XM_024997062.1
Latest version!
Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa238113 XM_010808687.3
Latest version!
Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa238114 XM_024997063.1
Latest version!
Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa48727
    Clone ID Related Accession (Same CDS sequence) XM_010808687.2 , XM_015464860.1
    Accession Version XM_010808687.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5028bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product A-kinase anchor protein 12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104241.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTTTTTA AATATTTAAT TTTTTTGAAT TATAATCATG TCTATCTTTT TCTTCCCCAC 
    CACCCCCTCT TTCTGTTAAT AGTTGGACAA AGAGAGTCTG AAGATGTGAG TGAAAGGGAC
    TCAGACAAAG AGATGGCTGC TAACTCAGCG GTGGTTCAAG ACATCACGAA GGAGGGAAAG
    GAGGAAATGC CTGAGATGAT GGAACCAATC CCTGCCTCGG AAAGCAGCGT GGATGAGCTG
    ATGCAACCCC CTGAGCCCCA GGCTAACGAC GTGGGATTTA AGAAGGTGTT CAAGTTTGTT
    GGCTTCAAAT TCACCGTGAA AAAGGATAAA ACCGAGAAAT CTGACAGCGT GCAGCTCCTC
    ACCGTCAAGA AGGATGAAAG TGAAGGGGCA GGAGAGTCCG ACGGGGCTGG TGACCACCCG
    GAGCCCAGCC GTGAGACGGG GGACGCAACA CCCAAAGACG GTGAACTGAA ACAGTCCACC
    GAGAAACCCG AAGAGGCGCC CCAACGTGAG CCGAGCTGCG CGGAAATCTC CCTTCAGACC
    GAGTCTGGTC AAGCAGCTGA GGAAGGCAAA GATGAAGGAG AAGAGAAACA AGAGAAAGAA
    ACCGCCAGAC CTGCAGACTC CCCGACTAGT CCCGTGGCCA GCGAAACCGC CTCGCCCTTC
    AGAAAATTCT TCACTCAAGG CTGGGCCGGC TGGCGCAAAA AGACCAGCTT CAGGAAGCCT
    CGGGAGGAGG AGCTGGAGGC TTCAGAGAAG AAAAAGGAAC AGGAGCCAGA AAAAGCCGAA
    GAAAATGAGA AGATGGAAGA GCCCTGCGAG CAGCCACCTG CCCAGGAGGC TCCTGAGAGC
    ACCCAGGATG CCAGGCTGTC GGCCGAGTAT GAAAAAGTGG AGCTGCCCCC TGAAGACCAA
    GTGCAGGCAC CTCCCGAAGA GAAACCAGCC CCGTTAGCAA CCGAAGTGTT TGATGAAAAG
    GTCGAGATTG TCGCCGAAGT CCACGTTAGC ACTGTAGAGA AGGACCAGGA AGAGCCGAAA
    GCCGAAGCGG AAGAAACAGT GGAGCTCTCG CCGCCCGAAA AGTCGACCGA GACGAGCGCC
    GACCTTCAGG AAGCCGAGTC GGCCGAGATG CTGAAGACAG AGGAAGATGC GGGTGCCCTG
    AGCGGGGACC ACTCCCAGGC AACCGAGCTC AGCGGGGACG CGAAATCGCC GTCCACGCCC
    CCCGAGGGCA CCGTGAGGGA GGCCGAAATG CTGTCCTCGC AGGAGAGAAT TAAGGTGCAG
    GGAAGCCCTT TGAAGAAACT ATTCACGAGC ACCGGCTTGA AAAAGCTCTC TGGAAAGAAA
    CAGAAAGGGA AACGAGGCGG CGACGAGGAG TCCGGGGAGC AGCCCTCAGC CTCCATGGAT
    TCTCCCGACA GTCCAGACGA ACTGAAGGGC GTTGAGAGCT CAGCCTCGTC TCCAGAAGAG
    CCCGAGGAGA TCACCTGCCT GGAGAAAGGG GTGGCGGAGG CCCCGGGACA GGATGGGGAG
    GCGGAGGAAG GGACCACTTC CGACGGGGAG AAGAAGAGGG AAGGTGGTGT CACTCCCTGG
    GCATCTTTCA AAAAGATGGT GACGCCCAAG AAACGCGTGC GAAGGCTCTC TGAAAGCGAT
    AAGGAGGACG AGGCAGACAA AGTCAAAAGC GCCACCCTGT CCTCCACAGA GAGCGCGGCC
    TCCGAAGTAC AGGAAGAGGC CAAAGGGAAC GGAGAGGAGC CGAAGCCCGA GGAGCCCAAG
    CGCAAGGTCG ACACCTCCGT GTCCTGGGAA GCGCTGATTT GCGTGGGGTC ATCCAAGAAG
    AGAGCCAGAA AAGCATCCTC TTCCGACGAT GAAGCAGGGC CGAAACCTCT TGGAGGGGAC
    AGCCAGAAAG CAGAGGAAGC TGGGAGGGAC AAGGAGCCGG GCCCGGAGGC AGCCGCTGCC
    GGTTCCCAGG ACCACGAGCA ACCGCCGGGA AGCTCCTCAC CCGAGCCGGC TGGCAGCCCA
    TCCGAAGGGG AGGGCGTCTC CACCTGGGAA ACCTTTAAAA GACTGGTCAC CTCGAGAAAA
    AAATCGAAGT CAAAACTGGA AGAGAGAAGC GAAGACTCTG TAGCTGGGTC TGGCGCAGAA
    CACGCAGCCT CAGAGGCTGA GCCTGGGAAA GAAGAGTCCT GGGTTTCCAT CCGGAAGTTT
    ATTCCTGGGC GAAGGAAGAA AAGGTCAGAT GGGAAGCAGG AGCCAGCCGC TGTCGAAGAG
    GCGGGGCCAG CGGAGGCTAA CGAGGACGAT GCGGACATCC CAGCCGTGGT CCCTCTGTCT
    GAATACGACG CAGTGGAAAG AGAGAAAACC GAAGCCCAGC CAGCCCCCAA GAGCCAGGAG
    GGGCCCGAGC AGAAGCAGGT GGATGTCGAC GTGTCGGAGG AGCTCAGTAA GAGCCTGGTT
    CACACCGTGT CGGTGGCTGT CGTGGATGGG ACAAGGGCCA TTACCAACAT CGAAGAAAGG
    GCACCCTCCT GGATCTCAGC TTCGGTGACA GAACCGCTGG AACAAGCCGA AGGTGAAGCC
    ACACCACCAA GCGAGGAGGT GCTTGAAAGA GAAGTCGTTG CGGAGGAAAC CCCCATCGTT
    ACCAAAGCGC TGCCAGAGAG CCAGGAGGTG GGTGATGACA CGATGGCCAG CGAGGTGGAA
    TTAACCTCAG AAGCTGTGAC AGCCGCTGAA ACCACAGAGG CCTCTGGTGC GGAAGAGACC
    ACCGACATGG TTTCGGCTGT CTCTCAGTTA ACCGACTCTC CAGACACCAC CGAGGAACCG
    ACACCAGTGC AAGAGGTGGA GGGAGGCGTG CCTGACGCGG AGGACCAGGC GAGAAGGACC
    CGAGAGGTGC TGCAGGCTGT TGCAGAAAAA GTTAGAGAAG AGTCACAGCT GCCAGATGAC
    GCCATCCAGA CAACCCAGAA AGGACAAGCA AAAATACTGG AGAAAGTGGA AGAGGCCGAG
    GAGGGATCTC AGGCCCTAGA TCTGAAGGAA GTGATGGATG GAGTATCCAA AGTGCTTGTC
    CAAGATACTG AAACTGAGAC TTTGACACAG GAGAAGGTGG TTGCAGAGGC CACCGCAGAA
    AGCTTGGGAG AAGTTCCTCC AGGCACAGAC AGTGCAGAGG CCAGGGAGCT GGCGAGCACT
    TGTCTTATTG AAACCGGGAC TGGGGTAGAA GCAGAGACTG CCCCAGAGCA GGCTGTTGCT
    CCTGACTCAA CTGAAACCCT CACGGACAGT GAGACCAATG GAAGCACCCC AGTAGCAGAT
    CTTGACGCTT CAAATCTAAG CCAGCCAGAC ACGATCATGG ATGTCCATGA AGATGGTGAG
    GTTCCAGCCG GCACTCAGTC CCAGGTCCCC GAAGGCGAGG TGCCTCCTGC AGTGAAAGAG
    CTGCCTGCAG CATCTTCTGA TTTTCAGTTG CAGGAGGAAA GGTCTTCAAA GATGGCAGAG
    GGTCTAGAAC ACACGGATGA AGAGGAGGGA ACAGTGGAGA CTGTAGCCAT CCTTTCAAAG
    ACTGAGATGA TTCAAGAGAC TGGCCAGTGC TTTGATGAGG AAGCCAAAGA AGAACCATCC
    ATGGAAGGAC TTGCCTTGTC TGCCGACACA GAAATAACTG AGAAAAAGAT AACTGAAGTT
    GTCCTTGAGG AGGACGTTAC TAAGAAAGTT GAATTTCAAG AGAATAATAT AGAACTCCAG
    AGTCCTGCTA AGTCTCTTCC AACCCCAGCA GAGAGAGAGA TGGTAGTTGA AGGGAAAAGG
    GAAACAGTGG AAGTGGAGGC AACTGAAGGG AATGAAGAGA AACTTGAGCA CGAACCAGCT
    GTGGCCGTAT GTGAAGAGCT CAGTAAGCAA CTGGTTCAGA CAGTGAATGT CACCATCATC
    GATGGGGAAA AGGAAGTCGT CAGTTTTGAA GGAAGTTCTC CCCTGCTGGC TTCCGAGGAA
    GAGGCATGCA CGGGAATCTC AGTTCAAAGC TCTGAGGCAG CATTAGCTCT AATAGCTGCA
    GCCGTGGAGG AGAAGGTCTT AGGAGAAGCT ATCAAGATTG TAGAAACAAC CGAGGCTTTG
    AAATCTGCAG AAGCTCATCT AGTACTGGAA GAAGAGTCCT CAGAAAAAGA CGAAGAGTTT
    CCTGCCCAGC CTGGGGAAGA AGATGTGGCA TCCACAGGGA CTGAGTCTCA AGCAGAATCA
    ATACCGACGA TAGTATCTAT TACGCCTGGA AAAGGCATCG ACGCTGACCT GGAAGGAGAT
    GAAACCACAT CACAGAAACA GGAGTTGGGT GGAGACGGTG AGCAGGTTGG TGGTCAGGAA
    GGCAGAGAGA GCAACACAGG AGAGGAAGAC GGCAAGGCGG AAAGTGAGAT TTCGAAACTT
    GAGAAGGAGA GCTGCAGACT TGTAGAGAGC GTGATTCAGA CAGCCGTCGA CCGTTTGGGG
    AGTACAGAAG AAACGGCCAC CGATGTCCAG ACCCAGGCTC AACTGGCAGA AGCCGACAGC
    CAGGAAGCTG GGCAGAAAAT GGAGAAAGAA GAAAGCAAAC CTCAGGCCTG CCTGGTGGAT
    GAAACACAAG CCGTAGAAGC CAAAGAGGCC AAAGAAGCCA AAGAGGAGTC CCCACTAAGC
    ACCGGCGAAC ATGCGCATCT CAGTGTTTCC AAAGATGCGA ATGAAGCTTC AGAGAAGGTG
    GCAGCCACTA GCATAGAAGG TTCCAGGGTA GACGACCAGC GGCTTGAAGA GGTAGCTCTC
    CCATCCCAGA AAAAGAGAGA AATCCTTGAA ACAGAGTCTG TGCTGCAAGA TGACGGCAGT
    GCCGGGTTTG GAGAAAGAAA GAAGTCCCCA TCTGAATCCC GAGAGGATGA AAAAGGTGAT
    GCTGCCGGTG ACCCTGGAAA CCAAACCTCA ATCCCAGAGG ATGCTGAGGC CTCGGGAGGC
    TCAACCAAAG AGTCCCTAGA TACAAATGGA CCCAAACTGA AAGAGAAGGG AGACAGCCAG
    GAGGAAAAGG TGCAGAGCGA GTCGGAAAAA GAGATGAAAA CACAAACACA GGAGGAGACA
    CAGAACCAAG AGAGAGATCT GGCAAAACCC GAACCCACGG AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806989.1
    CDS16..5043
    Translation

    Target ORF information:

    RefSeq Version XM_010808687.2
    Organism Bos taurus(cattle)
    Definition Bos taurus A-kinase anchoring protein 12 (AKAP12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808687.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTTTTTA AATATTTAAT TTTTTTGAAT TATAATCATG TCTATCTTTT TCTTCCCCAC 
    CACCCCCTCT TTCTGTTAAT AGTTGGACAA AGAGAGTCTG AAGATGTGAG TGAAAGGGAC
    TCAGACAAAG AGATGGCTGC TAACTCAGCG GTGGTTCAAG ACATCACGAA GGAGGGAAAG
    GAGGAAATGC CTGAGATGAT GGAACCAATC CCTGCCTCGG AAAGCAGCGT GGATGAGCTG
    ATGCAACCCC CTGAGCCCCA GGCTAACGAC GTGGGATTTA AGAAGGTGTT CAAGTTTGTT
    GGCTTCAAAT TCACCGTGAA AAAGGATAAA ACCGAGAAAT CTGACAGCGT GCAGCTCCTC
    ACCGTCAAGA AGGATGAAAG TGAAGGGGCA GGAGAGTCCG ACGGGGCTGG TGACCACCCG
    GAGCCCAGCC GTGAGACGGG GGACGCAACA CCCAAAGACG GTGAACTGAA ACAGTCCACC
    GAGAAACCCG AAGAGGCGCC CCAACGTGAG CCGAGCTGCG CGGAAATCTC CCTTCAGACC
    GAGTCTGGTC AAGCAGCTGA GGAAGGCAAA GATGAAGGAG AAGAGAAACA AGAGAAAGAA
    ACCGCCAGAC CTGCAGACTC CCCGACTAGT CCCGTGGCCA GCGAAACCGC CTCGCCCTTC
    AGAAAATTCT TCACTCAAGG CTGGGCCGGC TGGCGCAAAA AGACCAGCTT CAGGAAGCCT
    CGGGAGGAGG AGCTGGAGGC TTCAGAGAAG AAAAAGGAAC AGGAGCCAGA AAAAGCCGAA
    GAAAATGAGA AGATGGAAGA GCCCTGCGAG CAGCCACCTG CCCAGGAGGC TCCTGAGAGC
    ACCCAGGATG CCAGGCTGTC GGCCGAGTAT GAAAAAGTGG AGCTGCCCCC TGAAGACCAA
    GTGCAGGCAC CTCCCGAAGA GAAACCAGCC CCGTTAGCAA CCGAAGTGTT TGATGAAAAG
    GTCGAGATTG TCGCCGAAGT CCACGTTAGC ACTGTAGAGA AGGACCAGGA AGAGCCGAAA
    GCCGAAGCGG AAGAAACAGT GGAGCTCTCG CCGCCCGAAA AGTCGACCGA GACGAGCGCC
    GACCTTCAGG AAGCCGAGTC GGCCGAGATG CTGAAGACAG AGGAAGATGC GGGTGCCCTG
    AGCGGGGACC ACTCCCAGGC AACCGAGCTC AGCGGGGACG CGAAATCGCC GTCCACGCCC
    CCCGAGGGCA CCGTGAGGGA GGCCGAAATG CTGTCCTCGC AGGAGAGAAT TAAGGTGCAG
    GGAAGCCCTT TGAAGAAACT ATTCACGAGC ACCGGCTTGA AAAAGCTCTC TGGAAAGAAA
    CAGAAAGGGA AACGAGGCGG CGACGAGGAG TCCGGGGAGC AGCCCTCAGC CTCCATGGAT
    TCTCCCGACA GTCCAGACGA ACTGAAGGGC GTTGAGAGCT CAGCCTCGTC TCCAGAAGAG
    CCCGAGGAGA TCACCTGCCT GGAGAAAGGG GTGGCGGAGG CCCCGGGACA GGATGGGGAG
    GCGGAGGAAG GGACCACTTC CGACGGGGAG AAGAAGAGGG AAGGTGGTGT CACTCCCTGG
    GCATCTTTCA AAAAGATGGT GACGCCCAAG AAACGCGTGC GAAGGCTCTC TGAAAGCGAT
    AAGGAGGACG AGGCAGACAA AGTCAAAAGC GCCACCCTGT CCTCCACAGA GAGCGCGGCC
    TCCGAAGTAC AGGAAGAGGC CAAAGGGAAC GGAGAGGAGC CGAAGCCCGA GGAGCCCAAG
    CGCAAGGTCG ACACCTCCGT GTCCTGGGAA GCGCTGATTT GCGTGGGGTC ATCCAAGAAG
    AGAGCCAGAA AAGCATCCTC TTCCGACGAT GAAGCAGGGC CGAAACCTCT TGGAGGGGAC
    AGCCAGAAAG CAGAGGAAGC TGGGAGGGAC AAGGAGCCGG GCCCGGAGGC AGCCGCTGCC
    GGTTCCCAGG ACCACGAGCA ACCGCCGGGA AGCTCCTCAC CCGAGCCGGC TGGCAGCCCA
    TCCGAAGGGG AGGGCGTCTC CACCTGGGAA ACCTTTAAAA GACTGGTCAC CTCGAGAAAA
    AAATCGAAGT CAAAACTGGA AGAGAGAAGC GAAGACTCTG TAGCTGGGTC TGGCGCAGAA
    CACGCAGCCT CAGAGGCTGA GCCTGGGAAA GAAGAGTCCT GGGTTTCCAT CCGGAAGTTT
    ATTCCTGGGC GAAGGAAGAA AAGGTCAGAT GGGAAGCAGG AGCCAGCCGC TGTCGAAGAG
    GCGGGGCCAG CGGAGGCTAA CGAGGACGAT GCGGACATCC CAGCCGTGGT CCCTCTGTCT
    GAATACGACG CAGTGGAAAG AGAGAAAACC GAAGCCCAGC CAGCCCCCAA GAGCCAGGAG
    GGGCCCGAGC AGAAGCAGGT GGATGTCGAC GTGTCGGAGG AGCTCAGTAA GAGCCTGGTT
    CACACCGTGT CGGTGGCTGT CGTGGATGGG ACAAGGGCCA TTACCAACAT CGAAGAAAGG
    GCACCCTCCT GGATCTCAGC TTCGGTGACA GAACCGCTGG AACAAGCCGA AGGTGAAGCC
    ACACCACCAA GCGAGGAGGT GCTTGAAAGA GAAGTCGTTG CGGAGGAAAC CCCCATCGTT
    ACCAAAGCGC TGCCAGAGAG CCAGGAGGTG GGTGATGACA CGATGGCCAG CGAGGTGGAA
    TTAACCTCAG AAGCTGTGAC AGCCGCTGAA ACCACAGAGG CCTCTGGTGC GGAAGAGACC
    ACCGACATGG TTTCGGCTGT CTCTCAGTTA ACCGACTCTC CAGACACCAC CGAGGAACCG
    ACACCAGTGC AAGAGGTGGA GGGAGGCGTG CCTGACGCGG AGGACCAGGC GAGAAGGACC
    CGAGAGGTGC TGCAGGCTGT TGCAGAAAAA GTTAGAGAAG AGTCACAGCT GCCAGATGAC
    GCCATCCAGA CAACCCAGAA AGGACAAGCA AAAATACTGG AGAAAGTGGA AGAGGCCGAG
    GAGGGATCTC AGGCCCTAGA TCTGAAGGAA GTGATGGATG GAGTATCCAA AGTGCTTGTC
    CAAGATACTG AAACTGAGAC TTTGACACAG GAGAAGGTGG TTGCAGAGGC CACCGCAGAA
    AGCTTGGGAG AAGTTCCTCC AGGCACAGAC AGTGCAGAGG CCAGGGAGCT GGCGAGCACT
    TGTCTTATTG AAACCGGGAC TGGGGTAGAA GCAGAGACTG CCCCAGAGCA GGCTGTTGCT
    CCTGACTCAA CTGAAACCCT CACGGACAGT GAGACCAATG GAAGCACCCC AGTAGCAGAT
    CTTGACGCTT CAAATCTAAG CCAGCCAGAC ACGATCATGG ATGTCCATGA AGATGGTGAG
    GTTCCAGCCG GCACTCAGTC CCAGGTCCCC GAAGGCGAGG TGCCTCCTGC AGTGAAAGAG
    CTGCCTGCAG CATCTTCTGA TTTTCAGTTG CAGGAGGAAA GGTCTTCAAA GATGGCAGAG
    GGTCTAGAAC ACACGGATGA AGAGGAGGGA ACAGTGGAGA CTGTAGCCAT CCTTTCAAAG
    ACTGAGATGA TTCAAGAGAC TGGCCAGTGC TTTGATGAGG AAGCCAAAGA AGAACCATCC
    ATGGAAGGAC TTGCCTTGTC TGCCGACACA GAAATAACTG AGAAAAAGAT AACTGAAGTT
    GTCCTTGAGG AGGACGTTAC TAAGAAAGTT GAATTTCAAG AGAATAATAT AGAACTCCAG
    AGTCCTGCTA AGTCTCTTCC AACCCCAGCA GAGAGAGAGA TGGTAGTTGA AGGGAAAAGG
    GAAACAGTGG AAGTGGAGGC AACTGAAGGG AATGAAGAGA AACTTGAGCA CGAACCAGCT
    GTGGCCGTAT GTGAAGAGCT CAGTAAGCAA CTGGTTCAGA CAGTGAATGT CACCATCATC
    GATGGGGAAA AGGAAGTCGT CAGTTTTGAA GGAAGTTCTC CCCTGCTGGC TTCCGAGGAA
    GAGGCATGCA CGGGAATCTC AGTTCAAAGC TCTGAGGCAG CATTAGCTCT AATAGCTGCA
    GCCGTGGAGG AGAAGGTCTT AGGAGAAGCT ATCAAGATTG TAGAAACAAC CGAGGCTTTG
    AAATCTGCAG AAGCTCATCT AGTACTGGAA GAAGAGTCCT CAGAAAAAGA CGAAGAGTTT
    CCTGCCCAGC CTGGGGAAGA AGATGTGGCA TCCACAGGGA CTGAGTCTCA AGCAGAATCA
    ATACCGACGA TAGTATCTAT TACGCCTGGA AAAGGCATCG ACGCTGACCT GGAAGGAGAT
    GAAACCACAT CACAGAAACA GGAGTTGGGT GGAGACGGTG AGCAGGTTGG TGGTCAGGAA
    GGCAGAGAGA GCAACACAGG AGAGGAAGAC GGCAAGGCGG AAAGTGAGAT TTCGAAACTT
    GAGAAGGAGA GCTGCAGACT TGTAGAGAGC GTGATTCAGA CAGCCGTCGA CCGTTTGGGG
    AGTACAGAAG AAACGGCCAC CGATGTCCAG ACCCAGGCTC AACTGGCAGA AGCCGACAGC
    CAGGAAGCTG GGCAGAAAAT GGAGAAAGAA GAAAGCAAAC CTCAGGCCTG CCTGGTGGAT
    GAAACACAAG CCGTAGAAGC CAAAGAGGCC AAAGAAGCCA AAGAGGAGTC CCCACTAAGC
    ACCGGCGAAC ATGCGCATCT CAGTGTTTCC AAAGATGCGA ATGAAGCTTC AGAGAAGGTG
    GCAGCCACTA GCATAGAAGG TTCCAGGGTA GACGACCAGC GGCTTGAAGA GGTAGCTCTC
    CCATCCCAGA AAAAGAGAGA AATCCTTGAA ACAGAGTCTG TGCTGCAAGA TGACGGCAGT
    GCCGGGTTTG GAGAAAGAAA GAAGTCCCCA TCTGAATCCC GAGAGGATGA AAAAGGTGAT
    GCTGCCGGTG ACCCTGGAAA CCAAACCTCA ATCCCAGAGG ATGCTGAGGC CTCGGGAGGC
    TCAACCAAAG AGTCCCTAGA TACAAATGGA CCCAAACTGA AAGAGAAGGG AGACAGCCAG
    GAGGAAAAGG TGCAGAGCGA GTCGGAAAAA GAGATGAAAA CACAAACACA GGAGGAGACA
    CAGAACCAAG AGAGAGATCT GGCAAAACCC GAACCCACGG AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48727
    Clone ID Related Accession (Same CDS sequence) XM_010808687.2 , XM_015464860.1
    Accession Version XM_015464860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5028bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product A-kinase anchor protein 12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645764.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTTTTTA AATATTTAAT TTTTTTGAAT TATAATCATG TCTATCTTTT TCTTCCCCAC 
    CACCCCCTCT TTCTGTTAAT AGTTGGACAA AGAGAGTCTG AAGATGTGAG TGAAAGGGAC
    TCAGACAAAG AGATGGCTGC TAACTCAGCG GTGGTTCAAG ACATCACGAA GGAGGGAAAG
    GAGGAAATGC CTGAGATGAT GGAACCAATC CCTGCCTCGG AAAGCAGCGT GGATGAGCTG
    ATGCAACCCC CTGAGCCCCA GGCTAACGAC GTGGGATTTA AGAAGGTGTT CAAGTTTGTT
    GGCTTCAAAT TCACCGTGAA AAAGGATAAA ACCGAGAAAT CTGACAGCGT GCAGCTCCTC
    ACCGTCAAGA AGGATGAAAG TGAAGGGGCA GGAGAGTCCG ACGGGGCTGG TGACCACCCG
    GAGCCCAGCC GTGAGACGGG GGACGCAACA CCCAAAGACG GTGAACTGAA ACAGTCCACC
    GAGAAACCCG AAGAGGCGCC CCAACGTGAG CCGAGCTGCG CGGAAATCTC CCTTCAGACC
    GAGTCTGGTC AAGCAGCTGA GGAAGGCAAA GATGAAGGAG AAGAGAAACA AGAGAAAGAA
    ACCGCCAGAC CTGCAGACTC CCCGACTAGT CCCGTGGCCA GCGAAACCGC CTCGCCCTTC
    AGAAAATTCT TCACTCAAGG CTGGGCCGGC TGGCGCAAAA AGACCAGCTT CAGGAAGCCT
    CGGGAGGAGG AGCTGGAGGC TTCAGAGAAG AAAAAGGAAC AGGAGCCAGA AAAAGCCGAA
    GAAAATGAGA AGATGGAAGA GCCCTGCGAG CAGCCACCTG CCCAGGAGGC TCCTGAGAGC
    ACCCAGGATG CCAGGCTGTC GGCCGAGTAT GAAAAAGTGG AGCTGCCCCC TGAAGACCAA
    GTGCAGGCAC CTCCCGAAGA GAAACCAGCC CCGTTAGCAA CCGAAGTGTT TGATGAAAAG
    GTCGAGATTG TCGCCGAAGT CCACGTTAGC ACTGTAGAGA AGGACCAGGA AGAGCCGAAA
    GCCGAAGCGG AAGAAACAGT GGAGCTCTCG CCGCCCGAAA AGTCGACCGA GACGAGCGCC
    GACCTTCAGG AAGCCGAGTC GGCCGAGATG CTGAAGACAG AGGAAGATGC GGGTGCCCTG
    AGCGGGGACC ACTCCCAGGC AACCGAGCTC AGCGGGGACG CGAAATCGCC GTCCACGCCC
    CCCGAGGGCA CCGTGAGGGA GGCCGAAATG CTGTCCTCGC AGGAGAGAAT TAAGGTGCAG
    GGAAGCCCTT TGAAGAAACT ATTCACGAGC ACCGGCTTGA AAAAGCTCTC TGGAAAGAAA
    CAGAAAGGGA AACGAGGCGG CGACGAGGAG TCCGGGGAGC AGCCCTCAGC CTCCATGGAT
    TCTCCCGACA GTCCAGACGA ACTGAAGGGC GTTGAGAGCT CAGCCTCGTC TCCAGAAGAG
    CCCGAGGAGA TCACCTGCCT GGAGAAAGGG GTGGCGGAGG CCCCGGGACA GGATGGGGAG
    GCGGAGGAAG GGACCACTTC CGACGGGGAG AAGAAGAGGG AAGGTGGTGT CACTCCCTGG
    GCATCTTTCA AAAAGATGGT GACGCCCAAG AAACGCGTGC GAAGGCTCTC TGAAAGCGAT
    AAGGAGGACG AGGCAGACAA AGTCAAAAGC GCCACCCTGT CCTCCACAGA GAGCGCGGCC
    TCCGAAGTAC AGGAAGAGGC CAAAGGGAAC GGAGAGGAGC CGAAGCCCGA GGAGCCCAAG
    CGCAAGGTCG ACACCTCCGT GTCCTGGGAA GCGCTGATTT GCGTGGGGTC ATCCAAGAAG
    AGAGCCAGAA AAGCATCCTC TTCCGACGAT GAAGCAGGGC CGAAACCTCT TGGAGGGGAC
    AGCCAGAAAG CAGAGGAAGC TGGGAGGGAC AAGGAGCCGG GCCCGGAGGC AGCCGCTGCC
    GGTTCCCAGG ACCACGAGCA ACCGCCGGGA AGCTCCTCAC CCGAGCCGGC TGGCAGCCCA
    TCCGAAGGGG AGGGCGTCTC CACCTGGGAA ACCTTTAAAA GACTGGTCAC CTCGAGAAAA
    AAATCGAAGT CAAAACTGGA AGAGAGAAGC GAAGACTCTG TAGCTGGGTC TGGCGCAGAA
    CACGCAGCCT CAGAGGCTGA GCCTGGGAAA GAAGAGTCCT GGGTTTCCAT CCGGAAGTTT
    ATTCCTGGGC GAAGGAAGAA AAGGTCAGAT GGGAAGCAGG AGCCAGCCGC TGTCGAAGAG
    GCGGGGCCAG CGGAGGCTAA CGAGGACGAT GCGGACATCC CAGCCGTGGT CCCTCTGTCT
    GAATACGACG CAGTGGAAAG AGAGAAAACC GAAGCCCAGC CAGCCCCCAA GAGCCAGGAG
    GGGCCCGAGC AGAAGCAGGT GGATGTCGAC GTGTCGGAGG AGCTCAGTAA GAGCCTGGTT
    CACACCGTGT CGGTGGCTGT CGTGGATGGG ACAAGGGCCA TTACCAACAT CGAAGAAAGG
    GCACCCTCCT GGATCTCAGC TTCGGTGACA GAACCGCTGG AACAAGCCGA AGGTGAAGCC
    ACACCACCAA GCGAGGAGGT GCTTGAAAGA GAAGTCGTTG CGGAGGAAAC CCCCATCGTT
    ACCAAAGCGC TGCCAGAGAG CCAGGAGGTG GGTGATGACA CGATGGCCAG CGAGGTGGAA
    TTAACCTCAG AAGCTGTGAC AGCCGCTGAA ACCACAGAGG CCTCTGGTGC GGAAGAGACC
    ACCGACATGG TTTCGGCTGT CTCTCAGTTA ACCGACTCTC CAGACACCAC CGAGGAACCG
    ACACCAGTGC AAGAGGTGGA GGGAGGCGTG CCTGACGCGG AGGACCAGGC GAGAAGGACC
    CGAGAGGTGC TGCAGGCTGT TGCAGAAAAA GTTAGAGAAG AGTCACAGCT GCCAGATGAC
    GCCATCCAGA CAACCCAGAA AGGACAAGCA AAAATACTGG AGAAAGTGGA AGAGGCCGAG
    GAGGGATCTC AGGCCCTAGA TCTGAAGGAA GTGATGGATG GAGTATCCAA AGTGCTTGTC
    CAAGATACTG AAACTGAGAC TTTGACACAG GAGAAGGTGG TTGCAGAGGC CACCGCAGAA
    AGCTTGGGAG AAGTTCCTCC AGGCACAGAC AGTGCAGAGG CCAGGGAGCT GGCGAGCACT
    TGTCTTATTG AAACCGGGAC TGGGGTAGAA GCAGAGACTG CCCCAGAGCA GGCTGTTGCT
    CCTGACTCAA CTGAAACCCT CACGGACAGT GAGACCAATG GAAGCACCCC AGTAGCAGAT
    CTTGACGCTT CAAATCTAAG CCAGCCAGAC ACGATCATGG ATGTCCATGA AGATGGTGAG
    GTTCCAGCCG GCACTCAGTC CCAGGTCCCC GAAGGCGAGG TGCCTCCTGC AGTGAAAGAG
    CTGCCTGCAG CATCTTCTGA TTTTCAGTTG CAGGAGGAAA GGTCTTCAAA GATGGCAGAG
    GGTCTAGAAC ACACGGATGA AGAGGAGGGA ACAGTGGAGA CTGTAGCCAT CCTTTCAAAG
    ACTGAGATGA TTCAAGAGAC TGGCCAGTGC TTTGATGAGG AAGCCAAAGA AGAACCATCC
    ATGGAAGGAC TTGCCTTGTC TGCCGACACA GAAATAACTG AGAAAAAGAT AACTGAAGTT
    GTCCTTGAGG AGGACGTTAC TAAGAAAGTT GAATTTCAAG AGAATAATAT AGAACTCCAG
    AGTCCTGCTA AGTCTCTTCC AACCCCAGCA GAGAGAGAGA TGGTAGTTGA AGGGAAAAGG
    GAAACAGTGG AAGTGGAGGC AACTGAAGGG AATGAAGAGA AACTTGAGCA CGAACCAGCT
    GTGGCCGTAT GTGAAGAGCT CAGTAAGCAA CTGGTTCAGA CAGTGAATGT CACCATCATC
    GATGGGGAAA AGGAAGTCGT CAGTTTTGAA GGAAGTTCTC CCCTGCTGGC TTCCGAGGAA
    GAGGCATGCA CGGGAATCTC AGTTCAAAGC TCTGAGGCAG CATTAGCTCT AATAGCTGCA
    GCCGTGGAGG AGAAGGTCTT AGGAGAAGCT ATCAAGATTG TAGAAACAAC CGAGGCTTTG
    AAATCTGCAG AAGCTCATCT AGTACTGGAA GAAGAGTCCT CAGAAAAAGA CGAAGAGTTT
    CCTGCCCAGC CTGGGGAAGA AGATGTGGCA TCCACAGGGA CTGAGTCTCA AGCAGAATCA
    ATACCGACGA TAGTATCTAT TACGCCTGGA AAAGGCATCG ACGCTGACCT GGAAGGAGAT
    GAAACCACAT CACAGAAACA GGAGTTGGGT GGAGACGGTG AGCAGGTTGG TGGTCAGGAA
    GGCAGAGAGA GCAACACAGG AGAGGAAGAC GGCAAGGCGG AAAGTGAGAT TTCGAAACTT
    GAGAAGGAGA GCTGCAGACT TGTAGAGAGC GTGATTCAGA CAGCCGTCGA CCGTTTGGGG
    AGTACAGAAG AAACGGCCAC CGATGTCCAG ACCCAGGCTC AACTGGCAGA AGCCGACAGC
    CAGGAAGCTG GGCAGAAAAT GGAGAAAGAA GAAAGCAAAC CTCAGGCCTG CCTGGTGGAT
    GAAACACAAG CCGTAGAAGC CAAAGAGGCC AAAGAAGCCA AAGAGGAGTC CCCACTAAGC
    ACCGGCGAAC ATGCGCATCT CAGTGTTTCC AAAGATGCGA ATGAAGCTTC AGAGAAGGTG
    GCAGCCACTA GCATAGAAGG TTCCAGGGTA GACGACCAGC GGCTTGAAGA GGTAGCTCTC
    CCATCCCAGA AAAAGAGAGA AATCCTTGAA ACAGAGTCTG TGCTGCAAGA TGACGGCAGT
    GCCGGGTTTG GAGAAAGAAA GAAGTCCCCA TCTGAATCCC GAGAGGATGA AAAAGGTGAT
    GCTGCCGGTG ACCCTGGAAA CCAAACCTCA ATCCCAGAGG ATGCTGAGGC CTCGGGAGGC
    TCAACCAAAG AGTCCCTAGA TACAAATGGA CCCAAACTGA AAGAGAAGGG AGACAGCCAG
    GAGGAAAAGG TGCAGAGCGA GTCGGAAAAA GAGATGAAAA CACAAACACA GGAGGAGACA
    CAGAACCAAG AGAGAGATCT GGCAAAACCC GAACCCACGG AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320346.1
    CDS16..5043
    Translation

    Target ORF information:

    RefSeq Version XM_015464860.1
    Organism Bos taurus(cattle)
    Definition Bos taurus A-kinase anchoring protein 12 (AKAP12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTTTTTA AATATTTAAT TTTTTTGAAT TATAATCATG TCTATCTTTT TCTTCCCCAC 
    CACCCCCTCT TTCTGTTAAT AGTTGGACAA AGAGAGTCTG AAGATGTGAG TGAAAGGGAC
    TCAGACAAAG AGATGGCTGC TAACTCAGCG GTGGTTCAAG ACATCACGAA GGAGGGAAAG
    GAGGAAATGC CTGAGATGAT GGAACCAATC CCTGCCTCGG AAAGCAGCGT GGATGAGCTG
    ATGCAACCCC CTGAGCCCCA GGCTAACGAC GTGGGATTTA AGAAGGTGTT CAAGTTTGTT
    GGCTTCAAAT TCACCGTGAA AAAGGATAAA ACCGAGAAAT CTGACAGCGT GCAGCTCCTC
    ACCGTCAAGA AGGATGAAAG TGAAGGGGCA GGAGAGTCCG ACGGGGCTGG TGACCACCCG
    GAGCCCAGCC GTGAGACGGG GGACGCAACA CCCAAAGACG GTGAACTGAA ACAGTCCACC
    GAGAAACCCG AAGAGGCGCC CCAACGTGAG CCGAGCTGCG CGGAAATCTC CCTTCAGACC
    GAGTCTGGTC AAGCAGCTGA GGAAGGCAAA GATGAAGGAG AAGAGAAACA AGAGAAAGAA
    ACCGCCAGAC CTGCAGACTC CCCGACTAGT CCCGTGGCCA GCGAAACCGC CTCGCCCTTC
    AGAAAATTCT TCACTCAAGG CTGGGCCGGC TGGCGCAAAA AGACCAGCTT CAGGAAGCCT
    CGGGAGGAGG AGCTGGAGGC TTCAGAGAAG AAAAAGGAAC AGGAGCCAGA AAAAGCCGAA
    GAAAATGAGA AGATGGAAGA GCCCTGCGAG CAGCCACCTG CCCAGGAGGC TCCTGAGAGC
    ACCCAGGATG CCAGGCTGTC GGCCGAGTAT GAAAAAGTGG AGCTGCCCCC TGAAGACCAA
    GTGCAGGCAC CTCCCGAAGA GAAACCAGCC CCGTTAGCAA CCGAAGTGTT TGATGAAAAG
    GTCGAGATTG TCGCCGAAGT CCACGTTAGC ACTGTAGAGA AGGACCAGGA AGAGCCGAAA
    GCCGAAGCGG AAGAAACAGT GGAGCTCTCG CCGCCCGAAA AGTCGACCGA GACGAGCGCC
    GACCTTCAGG AAGCCGAGTC GGCCGAGATG CTGAAGACAG AGGAAGATGC GGGTGCCCTG
    AGCGGGGACC ACTCCCAGGC AACCGAGCTC AGCGGGGACG CGAAATCGCC GTCCACGCCC
    CCCGAGGGCA CCGTGAGGGA GGCCGAAATG CTGTCCTCGC AGGAGAGAAT TAAGGTGCAG
    GGAAGCCCTT TGAAGAAACT ATTCACGAGC ACCGGCTTGA AAAAGCTCTC TGGAAAGAAA
    CAGAAAGGGA AACGAGGCGG CGACGAGGAG TCCGGGGAGC AGCCCTCAGC CTCCATGGAT
    TCTCCCGACA GTCCAGACGA ACTGAAGGGC GTTGAGAGCT CAGCCTCGTC TCCAGAAGAG
    CCCGAGGAGA TCACCTGCCT GGAGAAAGGG GTGGCGGAGG CCCCGGGACA GGATGGGGAG
    GCGGAGGAAG GGACCACTTC CGACGGGGAG AAGAAGAGGG AAGGTGGTGT CACTCCCTGG
    GCATCTTTCA AAAAGATGGT GACGCCCAAG AAACGCGTGC GAAGGCTCTC TGAAAGCGAT
    AAGGAGGACG AGGCAGACAA AGTCAAAAGC GCCACCCTGT CCTCCACAGA GAGCGCGGCC
    TCCGAAGTAC AGGAAGAGGC CAAAGGGAAC GGAGAGGAGC CGAAGCCCGA GGAGCCCAAG
    CGCAAGGTCG ACACCTCCGT GTCCTGGGAA GCGCTGATTT GCGTGGGGTC ATCCAAGAAG
    AGAGCCAGAA AAGCATCCTC TTCCGACGAT GAAGCAGGGC CGAAACCTCT TGGAGGGGAC
    AGCCAGAAAG CAGAGGAAGC TGGGAGGGAC AAGGAGCCGG GCCCGGAGGC AGCCGCTGCC
    GGTTCCCAGG ACCACGAGCA ACCGCCGGGA AGCTCCTCAC CCGAGCCGGC TGGCAGCCCA
    TCCGAAGGGG AGGGCGTCTC CACCTGGGAA ACCTTTAAAA GACTGGTCAC CTCGAGAAAA
    AAATCGAAGT CAAAACTGGA AGAGAGAAGC GAAGACTCTG TAGCTGGGTC TGGCGCAGAA
    CACGCAGCCT CAGAGGCTGA GCCTGGGAAA GAAGAGTCCT GGGTTTCCAT CCGGAAGTTT
    ATTCCTGGGC GAAGGAAGAA AAGGTCAGAT GGGAAGCAGG AGCCAGCCGC TGTCGAAGAG
    GCGGGGCCAG CGGAGGCTAA CGAGGACGAT GCGGACATCC CAGCCGTGGT CCCTCTGTCT
    GAATACGACG CAGTGGAAAG AGAGAAAACC GAAGCCCAGC CAGCCCCCAA GAGCCAGGAG
    GGGCCCGAGC AGAAGCAGGT GGATGTCGAC GTGTCGGAGG AGCTCAGTAA GAGCCTGGTT
    CACACCGTGT CGGTGGCTGT CGTGGATGGG ACAAGGGCCA TTACCAACAT CGAAGAAAGG
    GCACCCTCCT GGATCTCAGC TTCGGTGACA GAACCGCTGG AACAAGCCGA AGGTGAAGCC
    ACACCACCAA GCGAGGAGGT GCTTGAAAGA GAAGTCGTTG CGGAGGAAAC CCCCATCGTT
    ACCAAAGCGC TGCCAGAGAG CCAGGAGGTG GGTGATGACA CGATGGCCAG CGAGGTGGAA
    TTAACCTCAG AAGCTGTGAC AGCCGCTGAA ACCACAGAGG CCTCTGGTGC GGAAGAGACC
    ACCGACATGG TTTCGGCTGT CTCTCAGTTA ACCGACTCTC CAGACACCAC CGAGGAACCG
    ACACCAGTGC AAGAGGTGGA GGGAGGCGTG CCTGACGCGG AGGACCAGGC GAGAAGGACC
    CGAGAGGTGC TGCAGGCTGT TGCAGAAAAA GTTAGAGAAG AGTCACAGCT GCCAGATGAC
    GCCATCCAGA CAACCCAGAA AGGACAAGCA AAAATACTGG AGAAAGTGGA AGAGGCCGAG
    GAGGGATCTC AGGCCCTAGA TCTGAAGGAA GTGATGGATG GAGTATCCAA AGTGCTTGTC
    CAAGATACTG AAACTGAGAC TTTGACACAG GAGAAGGTGG TTGCAGAGGC CACCGCAGAA
    AGCTTGGGAG AAGTTCCTCC AGGCACAGAC AGTGCAGAGG CCAGGGAGCT GGCGAGCACT
    TGTCTTATTG AAACCGGGAC TGGGGTAGAA GCAGAGACTG CCCCAGAGCA GGCTGTTGCT
    CCTGACTCAA CTGAAACCCT CACGGACAGT GAGACCAATG GAAGCACCCC AGTAGCAGAT
    CTTGACGCTT CAAATCTAAG CCAGCCAGAC ACGATCATGG ATGTCCATGA AGATGGTGAG
    GTTCCAGCCG GCACTCAGTC CCAGGTCCCC GAAGGCGAGG TGCCTCCTGC AGTGAAAGAG
    CTGCCTGCAG CATCTTCTGA TTTTCAGTTG CAGGAGGAAA GGTCTTCAAA GATGGCAGAG
    GGTCTAGAAC ACACGGATGA AGAGGAGGGA ACAGTGGAGA CTGTAGCCAT CCTTTCAAAG
    ACTGAGATGA TTCAAGAGAC TGGCCAGTGC TTTGATGAGG AAGCCAAAGA AGAACCATCC
    ATGGAAGGAC TTGCCTTGTC TGCCGACACA GAAATAACTG AGAAAAAGAT AACTGAAGTT
    GTCCTTGAGG AGGACGTTAC TAAGAAAGTT GAATTTCAAG AGAATAATAT AGAACTCCAG
    AGTCCTGCTA AGTCTCTTCC AACCCCAGCA GAGAGAGAGA TGGTAGTTGA AGGGAAAAGG
    GAAACAGTGG AAGTGGAGGC AACTGAAGGG AATGAAGAGA AACTTGAGCA CGAACCAGCT
    GTGGCCGTAT GTGAAGAGCT CAGTAAGCAA CTGGTTCAGA CAGTGAATGT CACCATCATC
    GATGGGGAAA AGGAAGTCGT CAGTTTTGAA GGAAGTTCTC CCCTGCTGGC TTCCGAGGAA
    GAGGCATGCA CGGGAATCTC AGTTCAAAGC TCTGAGGCAG CATTAGCTCT AATAGCTGCA
    GCCGTGGAGG AGAAGGTCTT AGGAGAAGCT ATCAAGATTG TAGAAACAAC CGAGGCTTTG
    AAATCTGCAG AAGCTCATCT AGTACTGGAA GAAGAGTCCT CAGAAAAAGA CGAAGAGTTT
    CCTGCCCAGC CTGGGGAAGA AGATGTGGCA TCCACAGGGA CTGAGTCTCA AGCAGAATCA
    ATACCGACGA TAGTATCTAT TACGCCTGGA AAAGGCATCG ACGCTGACCT GGAAGGAGAT
    GAAACCACAT CACAGAAACA GGAGTTGGGT GGAGACGGTG AGCAGGTTGG TGGTCAGGAA
    GGCAGAGAGA GCAACACAGG AGAGGAAGAC GGCAAGGCGG AAAGTGAGAT TTCGAAACTT
    GAGAAGGAGA GCTGCAGACT TGTAGAGAGC GTGATTCAGA CAGCCGTCGA CCGTTTGGGG
    AGTACAGAAG AAACGGCCAC CGATGTCCAG ACCCAGGCTC AACTGGCAGA AGCCGACAGC
    CAGGAAGCTG GGCAGAAAAT GGAGAAAGAA GAAAGCAAAC CTCAGGCCTG CCTGGTGGAT
    GAAACACAAG CCGTAGAAGC CAAAGAGGCC AAAGAAGCCA AAGAGGAGTC CCCACTAAGC
    ACCGGCGAAC ATGCGCATCT CAGTGTTTCC AAAGATGCGA ATGAAGCTTC AGAGAAGGTG
    GCAGCCACTA GCATAGAAGG TTCCAGGGTA GACGACCAGC GGCTTGAAGA GGTAGCTCTC
    CCATCCCAGA AAAAGAGAGA AATCCTTGAA ACAGAGTCTG TGCTGCAAGA TGACGGCAGT
    GCCGGGTTTG GAGAAAGAAA GAAGTCCCCA TCTGAATCCC GAGAGGATGA AAAAGGTGAT
    GCTGCCGGTG ACCCTGGAAA CCAAACCTCA ATCCCAGAGG ATGCTGAGGC CTCGGGAGGC
    TCAACCAAAG AGTCCCTAGA TACAAATGGA CCCAAACTGA AAGAGAAGGG AGACAGCCAG
    GAGGAAAAGG TGCAGAGCGA GTCGGAAAAA GAGATGAAAA CACAAACACA GGAGGAGACA
    CAGAACCAAG AGAGAGATCT GGCAAAACCC GAACCCACGG AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238112
    Clone ID Related Accession (Same CDS sequence) XM_024997062.1
    Accession Version XM_024997062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product A-kinase anchor protein 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGCGCCG GGAGCTCCAC CGAGCAGCGG AGCCCGGAGC AGCCGGAGGC GGGGAGCGCG 
    ACGCCCGCCG AGCCGGAGCC CAGCGGCGGC GGCTCTGCGG CGGAGGCGGC GCCCGGCACG
    CCGGGAGACC CGGCCATCGC CCCTGCGGAC CCCGCCACCA AGCTCCTGCA GAAGAATGGC
    CAGCTGTCCA CTGTCAACAG CTTAGCTGAG CAAGGCGAGC TCGGCCTCCA GGAGGAAGCC
    CTGATTGGCC AAGAGGAGGA AGTGACTGTC ACAGATGTTG GACAAAGAGA GTCTGAAGAT
    GTGAGTGAAA GGGACTCAGA CAAAGAGATG GCTGCTAACT CAGCGGTGGT TCAAGACATC
    ACGAAGGAGG GAAAGGAGGA AATGCCTGAG ATGATGGAAC CAATCCCTGC CTCGGAAAGC
    AGCGTGGATG AGCTGATGCA ACCCCCTGAG CCCCAGGCTA ACGACGTGGG ATTTAAGAAG
    GTGTTCAAGT TTGTTGGCTT CAAATTCACC GTGAAAAAGG ATAAAACCGA GAAATCTGAC
    AGCGTGCAGC TCCTCACCGT CAAGAAGGAT GAAAGTGAAG GGGCAGGAGA GTCCGACGGG
    GCTGGTGACC ACCCGGAGCC CAGCCGTGAG ACGGGGGACG CAACACCCAA AGACGGTGAA
    CTGAAACAGT CCACCGAGAA ACCCGAAGAG GCGCCCCAAC GTGAGCCGAG CTGCGCGGAA
    ATCTCCCTTC AGACCGAGTC TGGTCAAGCA GCTGAGGAAG GCAAAGATGA AGGAGAAGAG
    AAACAAGAGA AAGAAAGCGC CAGACCTGCA GACTCCCCGA CTAGTCCCGT GGCCAGCGAA
    ACCGCCTCGC CCTTCAGAAA ATTCTTCACT CAAGGCTGGG CCGGCTGGCG CAAAAAGACC
    AGCTTCAGGA AGCCTCGGGA GGAGGAGCTG GAGGCTTCAG AGAAGAAAAA GGAACAGGAG
    CCAGAAAAAG CCGAAGAAAA TGAGAAGATG GAAGAGCCCT GCGAGCAGCC ACCTGCCCAG
    GAGGCTCCTG AGAGCACCCA GGATGCCAGG CTGTCGGCCG AGTATGAAAA AGTGGAGCTG
    CCCCCTGAAG ACCAAGTGCA GGCACCTCCC GAAGAGAAAC CAGCCCCGTT AGCAACCGAA
    GTGTTTGATG AAAAGGTCGA GATTGTCGCC GAAGTCCACG TTAGCACTGT AGAGAAGGAC
    CAGGAAGAGC CGAAAGCCGA AGCGGAAGAA ACAGTGGAGC TCTCGCCGCC CGAAAAGTCG
    ACCGAGACGA GCGCCGACCT TCAGGAAGCC GAGTCGGCCG AGATGCTGAA GACAGAGGAA
    GATGCGGGTG CCCTGAGCGG GGACCACTCC CAGGCAACCG AGCTCAGCGG GGACGCGAAA
    TCGCCGTCCA CGCCCCCCGA GGGCACCGTG AGGGAGGCCG AAATGCTGTC CTCGCAGGAG
    AGAATTAAGG TGCAGGGAAG CCCTTTGAAG AAACTATTCA CGAGCACCGG CTTGAAAAAG
    CTCTCTGGAA AGAAACAGAA AGGGAAACGA GGCGGCGACG AGGAGTCCGG GGAGCAGCCC
    TCAGCCTCCA TGGATTCTCC CGACAGTCCA GACGAACTGA AGGGCGTTGA GAGCTCAGCC
    TCGTCTCCAG AAGAGCCCGA GGAGATCACC TGCCTGGAGA AAGGGGTGGC GGAGGCCCCG
    GGACAGGATG GGGAGGCGGA GGAAGGGACC ACTTCCGACG GGGAGAAGAA GAGGGAAGGT
    GGTGTCACTC CCTGGGCATC TTTCAAAAAG ATGGTGACGC CCAAGAAACG CGTGCGAAGG
    CTCTCTGAAA GCGATAAGGA GGACGAGGCA GACAAAGTCA AAAGCGCCAC CCTGTCCTCC
    ACAGAGAGCG CGGCCTCCGA AGTACAGGAA GAGGCCAAAG GGAACGGAGA GGAGCCGAAG
    CCCGAGGAGC CCAAGCGCAA GGTCGACACC TCCGTGTCCT GGGAAGCGCT GATTTGCGTG
    GGGTCATCCA AGAAGAGAGC CAGAAAAGCA TCCTCTTCCG ACGATGAAGC AGGGCCGAAA
    CCTCTTGGAG GGGACAGCCA GAAAGCAGAG GAAGCTGGGA GGGACAAGGA GCCGGGCCCG
    GAGGCAGCCG CTGCCGGTTC CCAGGACCAC GAGCAACCGC CGGGAAGCTC CTCACCCGAG
    CCGGCTGGCA GCCCATCCGA AGGGGAGGGC GTCTCCACCT GGGAAACCTT TAAAAGACTG
    GTCACCTCGA GAAAAAAATC GAAGTCAAAA CTGGAAGAGA GAAGCGAAGA CTCTGTAGCT
    GGGTCTGGCG CAGAACACGC AGCCTCAGAG GCTGAGCCTG GGAAAGAAGA GTCCTGGGTT
    TCCATCCGGA AGTTTATTCC TGGGCGAAGG AAGAAAAGGT CAGATGGGAA GCAGGAGCCA
    GCCGCTGTCG AAGAGGCGGG GCCAGCGGAG GCTAACGAGG ACGATGCGGA CATCCCAGCC
    GTGGTCCCTC TGTCTGAATA CGACGCAGTG GAAAGAGAGA AAACCGAAGC CCAGCCAGCC
    CCCAAGAGCC AGGAGGGGCC CGAGCAGAAG CAGGTGGATG TCGACGTGTC GGAGGAGCTC
    AGTAAGAGCC TGGTTCACAC CGTGTCGGTG GCTGTCGTGG ATGGGACAAG GGCCATTACC
    AACATCGAAG AAAGGGCACC CTCCTGGATC TCAGCTTCGG TGACAGAACC GCTGGAACAA
    GCCGAAGGTG AAGCCACACC ACCAAGCGAG GAGGTGCTTG AAAGAGAAGT CGTTGCGGAG
    GAAACCCCCA TCGTTACCAA AGCGCTGCCA GAGAGCCAGG AGGTGGGTGA TGACACGATG
    GCCAGCGAGG TGGAATTAAC CTCAGAAGCT GTGACAGCCG CTGAAACCAC AGAGGCCTCT
    GGTGCGGAAG AGACCACCGA CATGGTTTCG GCTGTCTCTC AGTTAACCGA CTCTCCAGAC
    ACCACCGAGG AACCGACACC AGTGCAAGAG GTGGAGGGAG GCGTGCCTGA CGCGGAGGAC
    CAGGCGAGAA GGACCCGAGA GGTGCTGCAG GCTGTTGCAG AAAAAGTTAG AGAAGAGTCA
    CAGCTGCCAG ATGACGCCAT CCAGACAACC CAGAAAGGAC AAGCAAAAAT ACTGGAGAAA
    GTGGAAGAGG CCGAGGAGGG ATCTCAGGCC CTAGATCTGA AGGAAGTGAT GGATGGAGTA
    TCCAAAGTGC TTGTCCAAGA TACTGAAACT GAGACTTTGA CACAGGAGAA GGTGGTTGCA
    GAGGCCACCG CAGAAAGCTT GGGAGAAGTT CCTCCAGGCA CAGACAGTGC AGAGGCCAGG
    GAGCTGGCGA GCACTTGTCT TATTGAAACC GGGACTGGGG TAGAAGCAGA GACTGCCCCA
    GAGCAGGCTG TTGCTCCTGA CTCAACTGAA ACCCTCACGG ACAGTGAGAC CAATGGAAGC
    ACCCCAGTAG CAGATCTTGA CGCTTCAAAT CTAAGCCAGC CAGACACGAT CATGGATGTC
    CATGAAGATG GTGAGGTTCC AGCCGGCACT CAGTCCCAGG TCCCCGAAGG CGAGGTGCCT
    CCTGCAGTGA AAGAGCTGCC TGCAGCATCT TCTGATTTTC AGTTGCAGGA GGAAAGGTCT
    TCAAAGATGG CAGAGGGTCT AGAACACACG GATGAAGAGG AGGGAACAGT GGAGACTGTA
    GCCATCCTTT CAAAGACTGA GATGATTCAA GAGACTGGCC AGTGCTTTGA TGAGGAAGCC
    AAAGAAGAAC CATCCATGGA AGGACTTGCC TTGTCTGCCG ACACAGAAAT AACTGAGAAA
    AAGATAACTG AAGTTGTCCT TGAGGAGGAC GTTACTAAGA AAGTTGAATT TCAAGAGAAT
    AATATAGAAC TCCAGAGTCC TGCTAAGTCT CTTCCAACCC CAGCAGAGAG AGAGATGGTA
    GTTGAAGGGA AAAGGGAAAC AGTGGAAGTG GAGGCAACTG AAGGGAATGA AGAGAAACTT
    GAGCACGAAC CAGCTGTGGC CGTATGTGAA GAGCTCAGTA AGCAACTGGT TCAGACAGTG
    AATGTCACCA TCATCGATGG GGAAAAGGAA GTCGTCAGTT TTGAAGGAAG TTCTCCCCTG
    CTGGCTTCCG AGGAAGAGGC ATGCACGGGA ATCTCAGTTC AAAGCTCTGA GGCAGCATTA
    GCTCTAATAG CTGCAGCCGT GGAGGAGAAG GTCTTAGGAG AAGCTATCAA GATTGTAGAA
    ACAACCGAGG CTTTGAAATC TGCAGAAGCT CATCTAGTAC TGGAAGAAGA GTCCTCAGAA
    AAAGACGAAG AGTTTCCTGC CCAGCCTGGG GAAGAAGATG TGGCATCCAC AGGGACTGAG
    TCTCAAGCAG AATCAATACC GACGATAGTA TCTATTACGC CTGGAAAAGG CATCGACGCT
    GACCTGGAAG GAGATGAAAC CACATCACAG AAACAGGAGT TGGGTGGAGA CGGTGAGCAG
    GTTGGTGGTC AGGAAGGCAG AGAGAGCAAC ACAGGAGAGG AAGACGGCAA GGCGGAAAGT
    GAGATTTCGA AACTTGAGAA GGAGAGCTGC AGACTTGTAG AGAGCGTGAT TCAGACAGCC
    GTCGACCGTT TGGGGAGTAC AGAAGAAACG GCCACCGATG TCCAGACCCA GGCTCAACTG
    GCAGAAGCCG ACAGCCAGGA AGCTGGGCAG AAAATGGAGA AAGAAGAAAG CAAACCTCAG
    GCCTGCCTGG TGGATGAAAC ACAAGCCGTA GAAGCCAAAG AGGCCAAAGA AGCCAAAGAG
    GAGTCCCCAC TAAGCACCGG CGAACATGCG CATCTCAGTG TTTCCAAAGA TGCGAATGAA
    GCTTCAGAGA AGGTGGCAGC CACTAGCATA GAAGGTTCCA GGGTAGACGA CCAGCGGCTT
    GAAGAGGTAG CTCTCCCATC CCAGAAAAAG AGAGAAATCC TTGAAACAGA GTCTGTGCTG
    CAAGATGACG GCAGTGCCGG GTTTGGAGAA AGAAAGAAGT CCCCATCTGA ATCCCGAGAG
    GATGAAAAAG GTGATGCTGC CGGTGACCCT GGAAACCAAA CCTCAATCCC AGAGGATGCT
    GAGGCCTCGG GAGGCTCAAC CAAAGAGTCC CTAGATACAA ATGGACCCAA ACTGAAAGAG
    AAGGGAGACA GCCAGGAGGA AAAGGTGCAG AGCGAGTCGG AAAAAGAGAT GAAAACACAA
    ACACAGGAGG AGACACAGAA CCAAGAGAGA GATCTGGCAA AACCCGAACC CACGGAATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852830.1
    CDS252..5474
    Translation

    Target ORF information:

    RefSeq Version XM_024997062.1
    Organism Bos taurus(cattle)
    Definition Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGCGCCG GGAGCTCCAC CGAGCAGCGG AGCCCGGAGC AGCCGGAGGC GGGGAGCGCG 
    ACGCCCGCCG AGCCGGAGCC CAGCGGCGGC GGCTCTGCGG CGGAGGCGGC GCCCGGCACG
    CCGGGAGACC CGGCCATCGC CCCTGCGGAC CCCGCCACCA AGCTCCTGCA GAAGAATGGC
    CAGCTGTCCA CTGTCAACAG CTTAGCTGAG CAAGGCGAGC TCGGCCTCCA GGAGGAAGCC
    CTGATTGGCC AAGAGGAGGA AGTGACTGTC ACAGATGTTG GACAAAGAGA GTCTGAAGAT
    GTGAGTGAAA GGGACTCAGA CAAAGAGATG GCTGCTAACT CAGCGGTGGT TCAAGACATC
    ACGAAGGAGG GAAAGGAGGA AATGCCTGAG ATGATGGAAC CAATCCCTGC CTCGGAAAGC
    AGCGTGGATG AGCTGATGCA ACCCCCTGAG CCCCAGGCTA ACGACGTGGG ATTTAAGAAG
    GTGTTCAAGT TTGTTGGCTT CAAATTCACC GTGAAAAAGG ATAAAACCGA GAAATCTGAC
    AGCGTGCAGC TCCTCACCGT CAAGAAGGAT GAAAGTGAAG GGGCAGGAGA GTCCGACGGG
    GCTGGTGACC ACCCGGAGCC CAGCCGTGAG ACGGGGGACG CAACACCCAA AGACGGTGAA
    CTGAAACAGT CCACCGAGAA ACCCGAAGAG GCGCCCCAAC GTGAGCCGAG CTGCGCGGAA
    ATCTCCCTTC AGACCGAGTC TGGTCAAGCA GCTGAGGAAG GCAAAGATGA AGGAGAAGAG
    AAACAAGAGA AAGAAAGCGC CAGACCTGCA GACTCCCCGA CTAGTCCCGT GGCCAGCGAA
    ACCGCCTCGC CCTTCAGAAA ATTCTTCACT CAAGGCTGGG CCGGCTGGCG CAAAAAGACC
    AGCTTCAGGA AGCCTCGGGA GGAGGAGCTG GAGGCTTCAG AGAAGAAAAA GGAACAGGAG
    CCAGAAAAAG CCGAAGAAAA TGAGAAGATG GAAGAGCCCT GCGAGCAGCC ACCTGCCCAG
    GAGGCTCCTG AGAGCACCCA GGATGCCAGG CTGTCGGCCG AGTATGAAAA AGTGGAGCTG
    CCCCCTGAAG ACCAAGTGCA GGCACCTCCC GAAGAGAAAC CAGCCCCGTT AGCAACCGAA
    GTGTTTGATG AAAAGGTCGA GATTGTCGCC GAAGTCCACG TTAGCACTGT AGAGAAGGAC
    CAGGAAGAGC CGAAAGCCGA AGCGGAAGAA ACAGTGGAGC TCTCGCCGCC CGAAAAGTCG
    ACCGAGACGA GCGCCGACCT TCAGGAAGCC GAGTCGGCCG AGATGCTGAA GACAGAGGAA
    GATGCGGGTG CCCTGAGCGG GGACCACTCC CAGGCAACCG AGCTCAGCGG GGACGCGAAA
    TCGCCGTCCA CGCCCCCCGA GGGCACCGTG AGGGAGGCCG AAATGCTGTC CTCGCAGGAG
    AGAATTAAGG TGCAGGGAAG CCCTTTGAAG AAACTATTCA CGAGCACCGG CTTGAAAAAG
    CTCTCTGGAA AGAAACAGAA AGGGAAACGA GGCGGCGACG AGGAGTCCGG GGAGCAGCCC
    TCAGCCTCCA TGGATTCTCC CGACAGTCCA GACGAACTGA AGGGCGTTGA GAGCTCAGCC
    TCGTCTCCAG AAGAGCCCGA GGAGATCACC TGCCTGGAGA AAGGGGTGGC GGAGGCCCCG
    GGACAGGATG GGGAGGCGGA GGAAGGGACC ACTTCCGACG GGGAGAAGAA GAGGGAAGGT
    GGTGTCACTC CCTGGGCATC TTTCAAAAAG ATGGTGACGC CCAAGAAACG CGTGCGAAGG
    CTCTCTGAAA GCGATAAGGA GGACGAGGCA GACAAAGTCA AAAGCGCCAC CCTGTCCTCC
    ACAGAGAGCG CGGCCTCCGA AGTACAGGAA GAGGCCAAAG GGAACGGAGA GGAGCCGAAG
    CCCGAGGAGC CCAAGCGCAA GGTCGACACC TCCGTGTCCT GGGAAGCGCT GATTTGCGTG
    GGGTCATCCA AGAAGAGAGC CAGAAAAGCA TCCTCTTCCG ACGATGAAGC AGGGCCGAAA
    CCTCTTGGAG GGGACAGCCA GAAAGCAGAG GAAGCTGGGA GGGACAAGGA GCCGGGCCCG
    GAGGCAGCCG CTGCCGGTTC CCAGGACCAC GAGCAACCGC CGGGAAGCTC CTCACCCGAG
    CCGGCTGGCA GCCCATCCGA AGGGGAGGGC GTCTCCACCT GGGAAACCTT TAAAAGACTG
    GTCACCTCGA GAAAAAAATC GAAGTCAAAA CTGGAAGAGA GAAGCGAAGA CTCTGTAGCT
    GGGTCTGGCG CAGAACACGC AGCCTCAGAG GCTGAGCCTG GGAAAGAAGA GTCCTGGGTT
    TCCATCCGGA AGTTTATTCC TGGGCGAAGG AAGAAAAGGT CAGATGGGAA GCAGGAGCCA
    GCCGCTGTCG AAGAGGCGGG GCCAGCGGAG GCTAACGAGG ACGATGCGGA CATCCCAGCC
    GTGGTCCCTC TGTCTGAATA CGACGCAGTG GAAAGAGAGA AAACCGAAGC CCAGCCAGCC
    CCCAAGAGCC AGGAGGGGCC CGAGCAGAAG CAGGTGGATG TCGACGTGTC GGAGGAGCTC
    AGTAAGAGCC TGGTTCACAC CGTGTCGGTG GCTGTCGTGG ATGGGACAAG GGCCATTACC
    AACATCGAAG AAAGGGCACC CTCCTGGATC TCAGCTTCGG TGACAGAACC GCTGGAACAA
    GCCGAAGGTG AAGCCACACC ACCAAGCGAG GAGGTGCTTG AAAGAGAAGT CGTTGCGGAG
    GAAACCCCCA TCGTTACCAA AGCGCTGCCA GAGAGCCAGG AGGTGGGTGA TGACACGATG
    GCCAGCGAGG TGGAATTAAC CTCAGAAGCT GTGACAGCCG CTGAAACCAC AGAGGCCTCT
    GGTGCGGAAG AGACCACCGA CATGGTTTCG GCTGTCTCTC AGTTAACCGA CTCTCCAGAC
    ACCACCGAGG AACCGACACC AGTGCAAGAG GTGGAGGGAG GCGTGCCTGA CGCGGAGGAC
    CAGGCGAGAA GGACCCGAGA GGTGCTGCAG GCTGTTGCAG AAAAAGTTAG AGAAGAGTCA
    CAGCTGCCAG ATGACGCCAT CCAGACAACC CAGAAAGGAC AAGCAAAAAT ACTGGAGAAA
    GTGGAAGAGG CCGAGGAGGG ATCTCAGGCC CTAGATCTGA AGGAAGTGAT GGATGGAGTA
    TCCAAAGTGC TTGTCCAAGA TACTGAAACT GAGACTTTGA CACAGGAGAA GGTGGTTGCA
    GAGGCCACCG CAGAAAGCTT GGGAGAAGTT CCTCCAGGCA CAGACAGTGC AGAGGCCAGG
    GAGCTGGCGA GCACTTGTCT TATTGAAACC GGGACTGGGG TAGAAGCAGA GACTGCCCCA
    GAGCAGGCTG TTGCTCCTGA CTCAACTGAA ACCCTCACGG ACAGTGAGAC CAATGGAAGC
    ACCCCAGTAG CAGATCTTGA CGCTTCAAAT CTAAGCCAGC CAGACACGAT CATGGATGTC
    CATGAAGATG GTGAGGTTCC AGCCGGCACT CAGTCCCAGG TCCCCGAAGG CGAGGTGCCT
    CCTGCAGTGA AAGAGCTGCC TGCAGCATCT TCTGATTTTC AGTTGCAGGA GGAAAGGTCT
    TCAAAGATGG CAGAGGGTCT AGAACACACG GATGAAGAGG AGGGAACAGT GGAGACTGTA
    GCCATCCTTT CAAAGACTGA GATGATTCAA GAGACTGGCC AGTGCTTTGA TGAGGAAGCC
    AAAGAAGAAC CATCCATGGA AGGACTTGCC TTGTCTGCCG ACACAGAAAT AACTGAGAAA
    AAGATAACTG AAGTTGTCCT TGAGGAGGAC GTTACTAAGA AAGTTGAATT TCAAGAGAAT
    AATATAGAAC TCCAGAGTCC TGCTAAGTCT CTTCCAACCC CAGCAGAGAG AGAGATGGTA
    GTTGAAGGGA AAAGGGAAAC AGTGGAAGTG GAGGCAACTG AAGGGAATGA AGAGAAACTT
    GAGCACGAAC CAGCTGTGGC CGTATGTGAA GAGCTCAGTA AGCAACTGGT TCAGACAGTG
    AATGTCACCA TCATCGATGG GGAAAAGGAA GTCGTCAGTT TTGAAGGAAG TTCTCCCCTG
    CTGGCTTCCG AGGAAGAGGC ATGCACGGGA ATCTCAGTTC AAAGCTCTGA GGCAGCATTA
    GCTCTAATAG CTGCAGCCGT GGAGGAGAAG GTCTTAGGAG AAGCTATCAA GATTGTAGAA
    ACAACCGAGG CTTTGAAATC TGCAGAAGCT CATCTAGTAC TGGAAGAAGA GTCCTCAGAA
    AAAGACGAAG AGTTTCCTGC CCAGCCTGGG GAAGAAGATG TGGCATCCAC AGGGACTGAG
    TCTCAAGCAG AATCAATACC GACGATAGTA TCTATTACGC CTGGAAAAGG CATCGACGCT
    GACCTGGAAG GAGATGAAAC CACATCACAG AAACAGGAGT TGGGTGGAGA CGGTGAGCAG
    GTTGGTGGTC AGGAAGGCAG AGAGAGCAAC ACAGGAGAGG AAGACGGCAA GGCGGAAAGT
    GAGATTTCGA AACTTGAGAA GGAGAGCTGC AGACTTGTAG AGAGCGTGAT TCAGACAGCC
    GTCGACCGTT TGGGGAGTAC AGAAGAAACG GCCACCGATG TCCAGACCCA GGCTCAACTG
    GCAGAAGCCG ACAGCCAGGA AGCTGGGCAG AAAATGGAGA AAGAAGAAAG CAAACCTCAG
    GCCTGCCTGG TGGATGAAAC ACAAGCCGTA GAAGCCAAAG AGGCCAAAGA AGCCAAAGAG
    GAGTCCCCAC TAAGCACCGG CGAACATGCG CATCTCAGTG TTTCCAAAGA TGCGAATGAA
    GCTTCAGAGA AGGTGGCAGC CACTAGCATA GAAGGTTCCA GGGTAGACGA CCAGCGGCTT
    GAAGAGGTAG CTCTCCCATC CCAGAAAAAG AGAGAAATCC TTGAAACAGA GTCTGTGCTG
    CAAGATGACG GCAGTGCCGG GTTTGGAGAA AGAAAGAAGT CCCCATCTGA ATCCCGAGAG
    GATGAAAAAG GTGATGCTGC CGGTGACCCT GGAAACCAAA CCTCAATCCC AGAGGATGCT
    GAGGCCTCGG GAGGCTCAAC CAAAGAGTCC CTAGATACAA ATGGACCCAA ACTGAAAGAG
    AAGGGAGACA GCCAGGAGGA AAAGGTGCAG AGCGAGTCGG AAAAAGAGAT GAAAACACAA
    ACACAGGAGG AGACACAGAA CCAAGAGAGA GATCTGGCAA AACCCGAACC CACGGAATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238113
    Clone ID Related Accession (Same CDS sequence) XM_010808687.3
    Accession Version XM_010808687.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product A-kinase anchor protein 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGCTGG GCGTCCTCAC GATCACAGTT GGACAAAGAG AGTCTGAAGA TGTGAGTGAA 
    AGGGACTCAG ACAAAGAGAT GGCTGCTAAC TCAGCGGTGG TTCAAGACAT CACGAAGGAG
    GGAAAGGAGG AAATGCCTGA GATGATGGAA CCAATCCCTG CCTCGGAAAG CAGCGTGGAT
    GAGCTGATGC AACCCCCTGA GCCCCAGGCT AACGACGTGG GATTTAAGAA GGTGTTCAAG
    TTTGTTGGCT TCAAATTCAC CGTGAAAAAG GATAAAACCG AGAAATCTGA CAGCGTGCAG
    CTCCTCACCG TCAAGAAGGA TGAAAGTGAA GGGGCAGGAG AGTCCGACGG GGCTGGTGAC
    CACCCGGAGC CCAGCCGTGA GACGGGGGAC GCAACACCCA AAGACGGTGA ACTGAAACAG
    TCCACCGAGA AACCCGAAGA GGCGCCCCAA CGTGAGCCGA GCTGCGCGGA AATCTCCCTT
    CAGACCGAGT CTGGTCAAGC AGCTGAGGAA GGCAAAGATG AAGGAGAAGA GAAACAAGAG
    AAAGAAAGCG CCAGACCTGC AGACTCCCCG ACTAGTCCCG TGGCCAGCGA AACCGCCTCG
    CCCTTCAGAA AATTCTTCAC TCAAGGCTGG GCCGGCTGGC GCAAAAAGAC CAGCTTCAGG
    AAGCCTCGGG AGGAGGAGCT GGAGGCTTCA GAGAAGAAAA AGGAACAGGA GCCAGAAAAA
    GCCGAAGAAA ATGAGAAGAT GGAAGAGCCC TGCGAGCAGC CACCTGCCCA GGAGGCTCCT
    GAGAGCACCC AGGATGCCAG GCTGTCGGCC GAGTATGAAA AAGTGGAGCT GCCCCCTGAA
    GACCAAGTGC AGGCACCTCC CGAAGAGAAA CCAGCCCCGT TAGCAACCGA AGTGTTTGAT
    GAAAAGGTCG AGATTGTCGC CGAAGTCCAC GTTAGCACTG TAGAGAAGGA CCAGGAAGAG
    CCGAAAGCCG AAGCGGAAGA AACAGTGGAG CTCTCGCCGC CCGAAAAGTC GACCGAGACG
    AGCGCCGACC TTCAGGAAGC CGAGTCGGCC GAGATGCTGA AGACAGAGGA AGATGCGGGT
    GCCCTGAGCG GGGACCACTC CCAGGCAACC GAGCTCAGCG GGGACGCGAA ATCGCCGTCC
    ACGCCCCCCG AGGGCACCGT GAGGGAGGCC GAAATGCTGT CCTCGCAGGA GAGAATTAAG
    GTGCAGGGAA GCCCTTTGAA GAAACTATTC ACGAGCACCG GCTTGAAAAA GCTCTCTGGA
    AAGAAACAGA AAGGGAAACG AGGCGGCGAC GAGGAGTCCG GGGAGCAGCC CTCAGCCTCC
    ATGGATTCTC CCGACAGTCC AGACGAACTG AAGGGCGTTG AGAGCTCAGC CTCGTCTCCA
    GAAGAGCCCG AGGAGATCAC CTGCCTGGAG AAAGGGGTGG CGGAGGCCCC GGGACAGGAT
    GGGGAGGCGG AGGAAGGGAC CACTTCCGAC GGGGAGAAGA AGAGGGAAGG TGGTGTCACT
    CCCTGGGCAT CTTTCAAAAA GATGGTGACG CCCAAGAAAC GCGTGCGAAG GCTCTCTGAA
    AGCGATAAGG AGGACGAGGC AGACAAAGTC AAAAGCGCCA CCCTGTCCTC CACAGAGAGC
    GCGGCCTCCG AAGTACAGGA AGAGGCCAAA GGGAACGGAG AGGAGCCGAA GCCCGAGGAG
    CCCAAGCGCA AGGTCGACAC CTCCGTGTCC TGGGAAGCGC TGATTTGCGT GGGGTCATCC
    AAGAAGAGAG CCAGAAAAGC ATCCTCTTCC GACGATGAAG CAGGGCCGAA ACCTCTTGGA
    GGGGACAGCC AGAAAGCAGA GGAAGCTGGG AGGGACAAGG AGCCGGGCCC GGAGGCAGCC
    GCTGCCGGTT CCCAGGACCA CGAGCAACCG CCGGGAAGCT CCTCACCCGA GCCGGCTGGC
    AGCCCATCCG AAGGGGAGGG CGTCTCCACC TGGGAAACCT TTAAAAGACT GGTCACCTCG
    AGAAAAAAAT CGAAGTCAAA ACTGGAAGAG AGAAGCGAAG ACTCTGTAGC TGGGTCTGGC
    GCAGAACACG CAGCCTCAGA GGCTGAGCCT GGGAAAGAAG AGTCCTGGGT TTCCATCCGG
    AAGTTTATTC CTGGGCGAAG GAAGAAAAGG TCAGATGGGA AGCAGGAGCC AGCCGCTGTC
    GAAGAGGCGG GGCCAGCGGA GGCTAACGAG GACGATGCGG ACATCCCAGC CGTGGTCCCT
    CTGTCTGAAT ACGACGCAGT GGAAAGAGAG AAAACCGAAG CCCAGCCAGC CCCCAAGAGC
    CAGGAGGGGC CCGAGCAGAA GCAGGTGGAT GTCGACGTGT CGGAGGAGCT CAGTAAGAGC
    CTGGTTCACA CCGTGTCGGT GGCTGTCGTG GATGGGACAA GGGCCATTAC CAACATCGAA
    GAAAGGGCAC CCTCCTGGAT CTCAGCTTCG GTGACAGAAC CGCTGGAACA AGCCGAAGGT
    GAAGCCACAC CACCAAGCGA GGAGGTGCTT GAAAGAGAAG TCGTTGCGGA GGAAACCCCC
    ATCGTTACCA AAGCGCTGCC AGAGAGCCAG GAGGTGGGTG ATGACACGAT GGCCAGCGAG
    GTGGAATTAA CCTCAGAAGC TGTGACAGCC GCTGAAACCA CAGAGGCCTC TGGTGCGGAA
    GAGACCACCG ACATGGTTTC GGCTGTCTCT CAGTTAACCG ACTCTCCAGA CACCACCGAG
    GAACCGACAC CAGTGCAAGA GGTGGAGGGA GGCGTGCCTG ACGCGGAGGA CCAGGCGAGA
    AGGACCCGAG AGGTGCTGCA GGCTGTTGCA GAAAAAGTTA GAGAAGAGTC ACAGCTGCCA
    GATGACGCCA TCCAGACAAC CCAGAAAGGA CAAGCAAAAA TACTGGAGAA AGTGGAAGAG
    GCCGAGGAGG GATCTCAGGC CCTAGATCTG AAGGAAGTGA TGGATGGAGT ATCCAAAGTG
    CTTGTCCAAG ATACTGAAAC TGAGACTTTG ACACAGGAGA AGGTGGTTGC AGAGGCCACC
    GCAGAAAGCT TGGGAGAAGT TCCTCCAGGC ACAGACAGTG CAGAGGCCAG GGAGCTGGCG
    AGCACTTGTC TTATTGAAAC CGGGACTGGG GTAGAAGCAG AGACTGCCCC AGAGCAGGCT
    GTTGCTCCTG ACTCAACTGA AACCCTCACG GACAGTGAGA CCAATGGAAG CACCCCAGTA
    GCAGATCTTG ACGCTTCAAA TCTAAGCCAG CCAGACACGA TCATGGATGT CCATGAAGAT
    GGTGAGGTTC CAGCCGGCAC TCAGTCCCAG GTCCCCGAAG GCGAGGTGCC TCCTGCAGTG
    AAAGAGCTGC CTGCAGCATC TTCTGATTTT CAGTTGCAGG AGGAAAGGTC TTCAAAGATG
    GCAGAGGGTC TAGAACACAC GGATGAAGAG GAGGGAACAG TGGAGACTGT AGCCATCCTT
    TCAAAGACTG AGATGATTCA AGAGACTGGC CAGTGCTTTG ATGAGGAAGC CAAAGAAGAA
    CCATCCATGG AAGGACTTGC CTTGTCTGCC GACACAGAAA TAACTGAGAA AAAGATAACT
    GAAGTTGTCC TTGAGGAGGA CGTTACTAAG AAAGTTGAAT TTCAAGAGAA TAATATAGAA
    CTCCAGAGTC CTGCTAAGTC TCTTCCAACC CCAGCAGAGA GAGAGATGGT AGTTGAAGGG
    AAAAGGGAAA CAGTGGAAGT GGAGGCAACT GAAGGGAATG AAGAGAAACT TGAGCACGAA
    CCAGCTGTGG CCGTATGTGA AGAGCTCAGT AAGCAACTGG TTCAGACAGT GAATGTCACC
    ATCATCGATG GGGAAAAGGA AGTCGTCAGT TTTGAAGGAA GTTCTCCCCT GCTGGCTTCC
    GAGGAAGAGG CATGCACGGG AATCTCAGTT CAAAGCTCTG AGGCAGCATT AGCTCTAATA
    GCTGCAGCCG TGGAGGAGAA GGTCTTAGGA GAAGCTATCA AGATTGTAGA AACAACCGAG
    GCTTTGAAAT CTGCAGAAGC TCATCTAGTA CTGGAAGAAG AGTCCTCAGA AAAAGACGAA
    GAGTTTCCTG CCCAGCCTGG GGAAGAAGAT GTGGCATCCA CAGGGACTGA GTCTCAAGCA
    GAATCAATAC CGACGATAGT ATCTATTACG CCTGGAAAAG GCATCGACGC TGACCTGGAA
    GGAGATGAAA CCACATCACA GAAACAGGAG TTGGGTGGAG ACGGTGAGCA GGTTGGTGGT
    CAGGAAGGCA GAGAGAGCAA CACAGGAGAG GAAGACGGCA AGGCGGAAAG TGAGATTTCG
    AAACTTGAGA AGGAGAGCTG CAGACTTGTA GAGAGCGTGA TTCAGACAGC CGTCGACCGT
    TTGGGGAGTA CAGAAGAAAC GGCCACCGAT GTCCAGACCC AGGCTCAACT GGCAGAAGCC
    GACAGCCAGG AAGCTGGGCA GAAAATGGAG AAAGAAGAAA GCAAACCTCA GGCCTGCCTG
    GTGGATGAAA CACAAGCCGT AGAAGCCAAA GAGGCCAAAG AAGCCAAAGA GGAGTCCCCA
    CTAAGCACCG GCGAACATGC GCATCTCAGT GTTTCCAAAG ATGCGAATGA AGCTTCAGAG
    AAGGTGGCAG CCACTAGCAT AGAAGGTTCC AGGGTAGACG ACCAGCGGCT TGAAGAGGTA
    GCTCTCCCAT CCCAGAAAAA GAGAGAAATC CTTGAAACAG AGTCTGTGCT GCAAGATGAC
    GGCAGTGCCG GGTTTGGAGA AAGAAAGAAG TCCCCATCTG AATCCCGAGA GGATGAAAAA
    GGTGATGCTG CCGGTGACCC TGGAAACCAA ACCTCAATCC CAGAGGATGC TGAGGCCTCG
    GGAGGCTCAA CCAAAGAGTC CCTAGATACA AATGGACCCA AACTGAAAGA GAAGGGAGAC
    AGCCAGGAGG AAAAGGTGCA GAGCGAGTCG GAAAAAGAGA TGAAAACACA AACACAGGAG
    GAGACACAGA ACCAAGAGAG AGATCTGGCA AAACCCGAAC CCACGGAATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806989.2
    CDS192..5165
    Translation

    Target ORF information:

    RefSeq Version XM_010808687.3
    Organism Bos taurus(cattle)
    Definition Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGCTGG GCGTCCTCAC GATCACAGTT GGACAAAGAG AGTCTGAAGA TGTGAGTGAA 
    AGGGACTCAG ACAAAGAGAT GGCTGCTAAC TCAGCGGTGG TTCAAGACAT CACGAAGGAG
    GGAAAGGAGG AAATGCCTGA GATGATGGAA CCAATCCCTG CCTCGGAAAG CAGCGTGGAT
    GAGCTGATGC AACCCCCTGA GCCCCAGGCT AACGACGTGG GATTTAAGAA GGTGTTCAAG
    TTTGTTGGCT TCAAATTCAC CGTGAAAAAG GATAAAACCG AGAAATCTGA CAGCGTGCAG
    CTCCTCACCG TCAAGAAGGA TGAAAGTGAA GGGGCAGGAG AGTCCGACGG GGCTGGTGAC
    CACCCGGAGC CCAGCCGTGA GACGGGGGAC GCAACACCCA AAGACGGTGA ACTGAAACAG
    TCCACCGAGA AACCCGAAGA GGCGCCCCAA CGTGAGCCGA GCTGCGCGGA AATCTCCCTT
    CAGACCGAGT CTGGTCAAGC AGCTGAGGAA GGCAAAGATG AAGGAGAAGA GAAACAAGAG
    AAAGAAAGCG CCAGACCTGC AGACTCCCCG ACTAGTCCCG TGGCCAGCGA AACCGCCTCG
    CCCTTCAGAA AATTCTTCAC TCAAGGCTGG GCCGGCTGGC GCAAAAAGAC CAGCTTCAGG
    AAGCCTCGGG AGGAGGAGCT GGAGGCTTCA GAGAAGAAAA AGGAACAGGA GCCAGAAAAA
    GCCGAAGAAA ATGAGAAGAT GGAAGAGCCC TGCGAGCAGC CACCTGCCCA GGAGGCTCCT
    GAGAGCACCC AGGATGCCAG GCTGTCGGCC GAGTATGAAA AAGTGGAGCT GCCCCCTGAA
    GACCAAGTGC AGGCACCTCC CGAAGAGAAA CCAGCCCCGT TAGCAACCGA AGTGTTTGAT
    GAAAAGGTCG AGATTGTCGC CGAAGTCCAC GTTAGCACTG TAGAGAAGGA CCAGGAAGAG
    CCGAAAGCCG AAGCGGAAGA AACAGTGGAG CTCTCGCCGC CCGAAAAGTC GACCGAGACG
    AGCGCCGACC TTCAGGAAGC CGAGTCGGCC GAGATGCTGA AGACAGAGGA AGATGCGGGT
    GCCCTGAGCG GGGACCACTC CCAGGCAACC GAGCTCAGCG GGGACGCGAA ATCGCCGTCC
    ACGCCCCCCG AGGGCACCGT GAGGGAGGCC GAAATGCTGT CCTCGCAGGA GAGAATTAAG
    GTGCAGGGAA GCCCTTTGAA GAAACTATTC ACGAGCACCG GCTTGAAAAA GCTCTCTGGA
    AAGAAACAGA AAGGGAAACG AGGCGGCGAC GAGGAGTCCG GGGAGCAGCC CTCAGCCTCC
    ATGGATTCTC CCGACAGTCC AGACGAACTG AAGGGCGTTG AGAGCTCAGC CTCGTCTCCA
    GAAGAGCCCG AGGAGATCAC CTGCCTGGAG AAAGGGGTGG CGGAGGCCCC GGGACAGGAT
    GGGGAGGCGG AGGAAGGGAC CACTTCCGAC GGGGAGAAGA AGAGGGAAGG TGGTGTCACT
    CCCTGGGCAT CTTTCAAAAA GATGGTGACG CCCAAGAAAC GCGTGCGAAG GCTCTCTGAA
    AGCGATAAGG AGGACGAGGC AGACAAAGTC AAAAGCGCCA CCCTGTCCTC CACAGAGAGC
    GCGGCCTCCG AAGTACAGGA AGAGGCCAAA GGGAACGGAG AGGAGCCGAA GCCCGAGGAG
    CCCAAGCGCA AGGTCGACAC CTCCGTGTCC TGGGAAGCGC TGATTTGCGT GGGGTCATCC
    AAGAAGAGAG CCAGAAAAGC ATCCTCTTCC GACGATGAAG CAGGGCCGAA ACCTCTTGGA
    GGGGACAGCC AGAAAGCAGA GGAAGCTGGG AGGGACAAGG AGCCGGGCCC GGAGGCAGCC
    GCTGCCGGTT CCCAGGACCA CGAGCAACCG CCGGGAAGCT CCTCACCCGA GCCGGCTGGC
    AGCCCATCCG AAGGGGAGGG CGTCTCCACC TGGGAAACCT TTAAAAGACT GGTCACCTCG
    AGAAAAAAAT CGAAGTCAAA ACTGGAAGAG AGAAGCGAAG ACTCTGTAGC TGGGTCTGGC
    GCAGAACACG CAGCCTCAGA GGCTGAGCCT GGGAAAGAAG AGTCCTGGGT TTCCATCCGG
    AAGTTTATTC CTGGGCGAAG GAAGAAAAGG TCAGATGGGA AGCAGGAGCC AGCCGCTGTC
    GAAGAGGCGG GGCCAGCGGA GGCTAACGAG GACGATGCGG ACATCCCAGC CGTGGTCCCT
    CTGTCTGAAT ACGACGCAGT GGAAAGAGAG AAAACCGAAG CCCAGCCAGC CCCCAAGAGC
    CAGGAGGGGC CCGAGCAGAA GCAGGTGGAT GTCGACGTGT CGGAGGAGCT CAGTAAGAGC
    CTGGTTCACA CCGTGTCGGT GGCTGTCGTG GATGGGACAA GGGCCATTAC CAACATCGAA
    GAAAGGGCAC CCTCCTGGAT CTCAGCTTCG GTGACAGAAC CGCTGGAACA AGCCGAAGGT
    GAAGCCACAC CACCAAGCGA GGAGGTGCTT GAAAGAGAAG TCGTTGCGGA GGAAACCCCC
    ATCGTTACCA AAGCGCTGCC AGAGAGCCAG GAGGTGGGTG ATGACACGAT GGCCAGCGAG
    GTGGAATTAA CCTCAGAAGC TGTGACAGCC GCTGAAACCA CAGAGGCCTC TGGTGCGGAA
    GAGACCACCG ACATGGTTTC GGCTGTCTCT CAGTTAACCG ACTCTCCAGA CACCACCGAG
    GAACCGACAC CAGTGCAAGA GGTGGAGGGA GGCGTGCCTG ACGCGGAGGA CCAGGCGAGA
    AGGACCCGAG AGGTGCTGCA GGCTGTTGCA GAAAAAGTTA GAGAAGAGTC ACAGCTGCCA
    GATGACGCCA TCCAGACAAC CCAGAAAGGA CAAGCAAAAA TACTGGAGAA AGTGGAAGAG
    GCCGAGGAGG GATCTCAGGC CCTAGATCTG AAGGAAGTGA TGGATGGAGT ATCCAAAGTG
    CTTGTCCAAG ATACTGAAAC TGAGACTTTG ACACAGGAGA AGGTGGTTGC AGAGGCCACC
    GCAGAAAGCT TGGGAGAAGT TCCTCCAGGC ACAGACAGTG CAGAGGCCAG GGAGCTGGCG
    AGCACTTGTC TTATTGAAAC CGGGACTGGG GTAGAAGCAG AGACTGCCCC AGAGCAGGCT
    GTTGCTCCTG ACTCAACTGA AACCCTCACG GACAGTGAGA CCAATGGAAG CACCCCAGTA
    GCAGATCTTG ACGCTTCAAA TCTAAGCCAG CCAGACACGA TCATGGATGT CCATGAAGAT
    GGTGAGGTTC CAGCCGGCAC TCAGTCCCAG GTCCCCGAAG GCGAGGTGCC TCCTGCAGTG
    AAAGAGCTGC CTGCAGCATC TTCTGATTTT CAGTTGCAGG AGGAAAGGTC TTCAAAGATG
    GCAGAGGGTC TAGAACACAC GGATGAAGAG GAGGGAACAG TGGAGACTGT AGCCATCCTT
    TCAAAGACTG AGATGATTCA AGAGACTGGC CAGTGCTTTG ATGAGGAAGC CAAAGAAGAA
    CCATCCATGG AAGGACTTGC CTTGTCTGCC GACACAGAAA TAACTGAGAA AAAGATAACT
    GAAGTTGTCC TTGAGGAGGA CGTTACTAAG AAAGTTGAAT TTCAAGAGAA TAATATAGAA
    CTCCAGAGTC CTGCTAAGTC TCTTCCAACC CCAGCAGAGA GAGAGATGGT AGTTGAAGGG
    AAAAGGGAAA CAGTGGAAGT GGAGGCAACT GAAGGGAATG AAGAGAAACT TGAGCACGAA
    CCAGCTGTGG CCGTATGTGA AGAGCTCAGT AAGCAACTGG TTCAGACAGT GAATGTCACC
    ATCATCGATG GGGAAAAGGA AGTCGTCAGT TTTGAAGGAA GTTCTCCCCT GCTGGCTTCC
    GAGGAAGAGG CATGCACGGG AATCTCAGTT CAAAGCTCTG AGGCAGCATT AGCTCTAATA
    GCTGCAGCCG TGGAGGAGAA GGTCTTAGGA GAAGCTATCA AGATTGTAGA AACAACCGAG
    GCTTTGAAAT CTGCAGAAGC TCATCTAGTA CTGGAAGAAG AGTCCTCAGA AAAAGACGAA
    GAGTTTCCTG CCCAGCCTGG GGAAGAAGAT GTGGCATCCA CAGGGACTGA GTCTCAAGCA
    GAATCAATAC CGACGATAGT ATCTATTACG CCTGGAAAAG GCATCGACGC TGACCTGGAA
    GGAGATGAAA CCACATCACA GAAACAGGAG TTGGGTGGAG ACGGTGAGCA GGTTGGTGGT
    CAGGAAGGCA GAGAGAGCAA CACAGGAGAG GAAGACGGCA AGGCGGAAAG TGAGATTTCG
    AAACTTGAGA AGGAGAGCTG CAGACTTGTA GAGAGCGTGA TTCAGACAGC CGTCGACCGT
    TTGGGGAGTA CAGAAGAAAC GGCCACCGAT GTCCAGACCC AGGCTCAACT GGCAGAAGCC
    GACAGCCAGG AAGCTGGGCA GAAAATGGAG AAAGAAGAAA GCAAACCTCA GGCCTGCCTG
    GTGGATGAAA CACAAGCCGT AGAAGCCAAA GAGGCCAAAG AAGCCAAAGA GGAGTCCCCA
    CTAAGCACCG GCGAACATGC GCATCTCAGT GTTTCCAAAG ATGCGAATGA AGCTTCAGAG
    AAGGTGGCAG CCACTAGCAT AGAAGGTTCC AGGGTAGACG ACCAGCGGCT TGAAGAGGTA
    GCTCTCCCAT CCCAGAAAAA GAGAGAAATC CTTGAAACAG AGTCTGTGCT GCAAGATGAC
    GGCAGTGCCG GGTTTGGAGA AAGAAAGAAG TCCCCATCTG AATCCCGAGA GGATGAAAAA
    GGTGATGCTG CCGGTGACCC TGGAAACCAA ACCTCAATCC CAGAGGATGC TGAGGCCTCG
    GGAGGCTCAA CCAAAGAGTC CCTAGATACA AATGGACCCA AACTGAAAGA GAAGGGAGAC
    AGCCAGGAGG AAAAGGTGCA GAGCGAGTCG GAAAAAGAGA TGAAAACACA AACACAGGAG
    GAGACACAGA ACCAAGAGAG AGATCTGGCA AAACCCGAAC CCACGGAATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238114
    Clone ID Related Accession (Same CDS sequence) XM_024997063.1
    Accession Version XM_024997063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product A-kinase anchor protein 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGT GGAACAGCCT CTGCTTTGGA CAAAGAGAGT CTGAAGATGT GAGTGAAAGG 
    GACTCAGACA AAGAGATGGC TGCTAACTCA GCGGTGGTTC AAGACATCAC GAAGGAGGGA
    AAGGAGGAAA TGCCTGAGAT GATGGAACCA ATCCCTGCCT CGGAAAGCAG CGTGGATGAG
    CTGATGCAAC CCCCTGAGCC CCAGGCTAAC GACGTGGGAT TTAAGAAGGT GTTCAAGTTT
    GTTGGCTTCA AATTCACCGT GAAAAAGGAT AAAACCGAGA AATCTGACAG CGTGCAGCTC
    CTCACCGTCA AGAAGGATGA AAGTGAAGGG GCAGGAGAGT CCGACGGGGC TGGTGACCAC
    CCGGAGCCCA GCCGTGAGAC GGGGGACGCA ACACCCAAAG ACGGTGAACT GAAACAGTCC
    ACCGAGAAAC CCGAAGAGGC GCCCCAACGT GAGCCGAGCT GCGCGGAAAT CTCCCTTCAG
    ACCGAGTCTG GTCAAGCAGC TGAGGAAGGC AAAGATGAAG GAGAAGAGAA ACAAGAGAAA
    GAAAGCGCCA GACCTGCAGA CTCCCCGACT AGTCCCGTGG CCAGCGAAAC CGCCTCGCCC
    TTCAGAAAAT TCTTCACTCA AGGCTGGGCC GGCTGGCGCA AAAAGACCAG CTTCAGGAAG
    CCTCGGGAGG AGGAGCTGGA GGCTTCAGAG AAGAAAAAGG AACAGGAGCC AGAAAAAGCC
    GAAGAAAATG AGAAGATGGA AGAGCCCTGC GAGCAGCCAC CTGCCCAGGA GGCTCCTGAG
    AGCACCCAGG ATGCCAGGCT GTCGGCCGAG TATGAAAAAG TGGAGCTGCC CCCTGAAGAC
    CAAGTGCAGG CACCTCCCGA AGAGAAACCA GCCCCGTTAG CAACCGAAGT GTTTGATGAA
    AAGGTCGAGA TTGTCGCCGA AGTCCACGTT AGCACTGTAG AGAAGGACCA GGAAGAGCCG
    AAAGCCGAAG CGGAAGAAAC AGTGGAGCTC TCGCCGCCCG AAAAGTCGAC CGAGACGAGC
    GCCGACCTTC AGGAAGCCGA GTCGGCCGAG ATGCTGAAGA CAGAGGAAGA TGCGGGTGCC
    CTGAGCGGGG ACCACTCCCA GGCAACCGAG CTCAGCGGGG ACGCGAAATC GCCGTCCACG
    CCCCCCGAGG GCACCGTGAG GGAGGCCGAA ATGCTGTCCT CGCAGGAGAG AATTAAGGTG
    CAGGGAAGCC CTTTGAAGAA ACTATTCACG AGCACCGGCT TGAAAAAGCT CTCTGGAAAG
    AAACAGAAAG GGAAACGAGG CGGCGACGAG GAGTCCGGGG AGCAGCCCTC AGCCTCCATG
    GATTCTCCCG ACAGTCCAGA CGAACTGAAG GGCGTTGAGA GCTCAGCCTC GTCTCCAGAA
    GAGCCCGAGG AGATCACCTG CCTGGAGAAA GGGGTGGCGG AGGCCCCGGG ACAGGATGGG
    GAGGCGGAGG AAGGGACCAC TTCCGACGGG GAGAAGAAGA GGGAAGGTGG TGTCACTCCC
    TGGGCATCTT TCAAAAAGAT GGTGACGCCC AAGAAACGCG TGCGAAGGCT CTCTGAAAGC
    GATAAGGAGG ACGAGGCAGA CAAAGTCAAA AGCGCCACCC TGTCCTCCAC AGAGAGCGCG
    GCCTCCGAAG TACAGGAAGA GGCCAAAGGG AACGGAGAGG AGCCGAAGCC CGAGGAGCCC
    AAGCGCAAGG TCGACACCTC CGTGTCCTGG GAAGCGCTGA TTTGCGTGGG GTCATCCAAG
    AAGAGAGCCA GAAAAGCATC CTCTTCCGAC GATGAAGCAG GGCCGAAACC TCTTGGAGGG
    GACAGCCAGA AAGCAGAGGA AGCTGGGAGG GACAAGGAGC CGGGCCCGGA GGCAGCCGCT
    GCCGGTTCCC AGGACCACGA GCAACCGCCG GGAAGCTCCT CACCCGAGCC GGCTGGCAGC
    CCATCCGAAG GGGAGGGCGT CTCCACCTGG GAAACCTTTA AAAGACTGGT CACCTCGAGA
    AAAAAATCGA AGTCAAAACT GGAAGAGAGA AGCGAAGACT CTGTAGCTGG GTCTGGCGCA
    GAACACGCAG CCTCAGAGGC TGAGCCTGGG AAAGAAGAGT CCTGGGTTTC CATCCGGAAG
    TTTATTCCTG GGCGAAGGAA GAAAAGGTCA GATGGGAAGC AGGAGCCAGC CGCTGTCGAA
    GAGGCGGGGC CAGCGGAGGC TAACGAGGAC GATGCGGACA TCCCAGCCGT GGTCCCTCTG
    TCTGAATACG ACGCAGTGGA AAGAGAGAAA ACCGAAGCCC AGCCAGCCCC CAAGAGCCAG
    GAGGGGCCCG AGCAGAAGCA GGTGGATGTC GACGTGTCGG AGGAGCTCAG TAAGAGCCTG
    GTTCACACCG TGTCGGTGGC TGTCGTGGAT GGGACAAGGG CCATTACCAA CATCGAAGAA
    AGGGCACCCT CCTGGATCTC AGCTTCGGTG ACAGAACCGC TGGAACAAGC CGAAGGTGAA
    GCCACACCAC CAAGCGAGGA GGTGCTTGAA AGAGAAGTCG TTGCGGAGGA AACCCCCATC
    GTTACCAAAG CGCTGCCAGA GAGCCAGGAG GTGGGTGATG ACACGATGGC CAGCGAGGTG
    GAATTAACCT CAGAAGCTGT GACAGCCGCT GAAACCACAG AGGCCTCTGG TGCGGAAGAG
    ACCACCGACA TGGTTTCGGC TGTCTCTCAG TTAACCGACT CTCCAGACAC CACCGAGGAA
    CCGACACCAG TGCAAGAGGT GGAGGGAGGC GTGCCTGACG CGGAGGACCA GGCGAGAAGG
    ACCCGAGAGG TGCTGCAGGC TGTTGCAGAA AAAGTTAGAG AAGAGTCACA GCTGCCAGAT
    GACGCCATCC AGACAACCCA GAAAGGACAA GCAAAAATAC TGGAGAAAGT GGAAGAGGCC
    GAGGAGGGAT CTCAGGCCCT AGATCTGAAG GAAGTGATGG ATGGAGTATC CAAAGTGCTT
    GTCCAAGATA CTGAAACTGA GACTTTGACA CAGGAGAAGG TGGTTGCAGA GGCCACCGCA
    GAAAGCTTGG GAGAAGTTCC TCCAGGCACA GACAGTGCAG AGGCCAGGGA GCTGGCGAGC
    ACTTGTCTTA TTGAAACCGG GACTGGGGTA GAAGCAGAGA CTGCCCCAGA GCAGGCTGTT
    GCTCCTGACT CAACTGAAAC CCTCACGGAC AGTGAGACCA ATGGAAGCAC CCCAGTAGCA
    GATCTTGACG CTTCAAATCT AAGCCAGCCA GACACGATCA TGGATGTCCA TGAAGATGGT
    GAGGTTCCAG CCGGCACTCA GTCCCAGGTC CCCGAAGGCG AGGTGCCTCC TGCAGTGAAA
    GAGCTGCCTG CAGCATCTTC TGATTTTCAG TTGCAGGAGG AAAGGTCTTC AAAGATGGCA
    GAGGGTCTAG AACACACGGA TGAAGAGGAG GGAACAGTGG AGACTGTAGC CATCCTTTCA
    AAGACTGAGA TGATTCAAGA GACTGGCCAG TGCTTTGATG AGGAAGCCAA AGAAGAACCA
    TCCATGGAAG GACTTGCCTT GTCTGCCGAC ACAGAAATAA CTGAGAAAAA GATAACTGAA
    GTTGTCCTTG AGGAGGACGT TACTAAGAAA GTTGAATTTC AAGAGAATAA TATAGAACTC
    CAGAGTCCTG CTAAGTCTCT TCCAACCCCA GCAGAGAGAG AGATGGTAGT TGAAGGGAAA
    AGGGAAACAG TGGAAGTGGA GGCAACTGAA GGGAATGAAG AGAAACTTGA GCACGAACCA
    GCTGTGGCCG TATGTGAAGA GCTCAGTAAG CAACTGGTTC AGACAGTGAA TGTCACCATC
    ATCGATGGGG AAAAGGAAGT CGTCAGTTTT GAAGGAAGTT CTCCCCTGCT GGCTTCCGAG
    GAAGAGGCAT GCACGGGAAT CTCAGTTCAA AGCTCTGAGG CAGCATTAGC TCTAATAGCT
    GCAGCCGTGG AGGAGAAGGT CTTAGGAGAA GCTATCAAGA TTGTAGAAAC AACCGAGGCT
    TTGAAATCTG CAGAAGCTCA TCTAGTACTG GAAGAAGAGT CCTCAGAAAA AGACGAAGAG
    TTTCCTGCCC AGCCTGGGGA AGAAGATGTG GCATCCACAG GGACTGAGTC TCAAGCAGAA
    TCAATACCGA CGATAGTATC TATTACGCCT GGAAAAGGCA TCGACGCTGA CCTGGAAGGA
    GATGAAACCA CATCACAGAA ACAGGAGTTG GGTGGAGACG GTGAGCAGGT TGGTGGTCAG
    GAAGGCAGAG AGAGCAACAC AGGAGAGGAA GACGGCAAGG CGGAAAGTGA GATTTCGAAA
    CTTGAGAAGG AGAGCTGCAG ACTTGTAGAG AGCGTGATTC AGACAGCCGT CGACCGTTTG
    GGGAGTACAG AAGAAACGGC CACCGATGTC CAGACCCAGG CTCAACTGGC AGAAGCCGAC
    AGCCAGGAAG CTGGGCAGAA AATGGAGAAA GAAGAAAGCA AACCTCAGGC CTGCCTGGTG
    GATGAAACAC AAGCCGTAGA AGCCAAAGAG GCCAAAGAAG CCAAAGAGGA GTCCCCACTA
    AGCACCGGCG AACATGCGCA TCTCAGTGTT TCCAAAGATG CGAATGAAGC TTCAGAGAAG
    GTGGCAGCCA CTAGCATAGA AGGTTCCAGG GTAGACGACC AGCGGCTTGA AGAGGTAGCT
    CTCCCATCCC AGAAAAAGAG AGAAATCCTT GAAACAGAGT CTGTGCTGCA AGATGACGGC
    AGTGCCGGGT TTGGAGAAAG AAAGAAGTCC CCATCTGAAT CCCGAGAGGA TGAAAAAGGT
    GATGCTGCCG GTGACCCTGG AAACCAAACC TCAATCCCAG AGGATGCTGA GGCCTCGGGA
    GGCTCAACCA AAGAGTCCCT AGATACAAAT GGACCCAAAC TGAAAGAGAA GGGAGACAGC
    CAGGAGGAAA AGGTGCAGAG CGAGTCGGAA AAAGAGATGA AAACACAAAC ACAGGAGGAG
    ACACAGAACC AAGAGAGAGA TCTGGCAAAA CCCGAACCCA CGGAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852831.1
    CDS82..5052
    Translation

    Target ORF information:

    RefSeq Version XM_024997063.1
    Organism Bos taurus(cattle)
    Definition Bos taurus A-kinase anchoring protein 12 (AKAP12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCCTGGT GGAACAGCCT CTGCTTTGGA CAAAGAGAGT CTGAAGATGT GAGTGAAAGG 
    GACTCAGACA AAGAGATGGC TGCTAACTCA GCGGTGGTTC AAGACATCAC GAAGGAGGGA
    AAGGAGGAAA TGCCTGAGAT GATGGAACCA ATCCCTGCCT CGGAAAGCAG CGTGGATGAG
    CTGATGCAAC CCCCTGAGCC CCAGGCTAAC GACGTGGGAT TTAAGAAGGT GTTCAAGTTT
    GTTGGCTTCA AATTCACCGT GAAAAAGGAT AAAACCGAGA AATCTGACAG CGTGCAGCTC
    CTCACCGTCA AGAAGGATGA AAGTGAAGGG GCAGGAGAGT CCGACGGGGC TGGTGACCAC
    CCGGAGCCCA GCCGTGAGAC GGGGGACGCA ACACCCAAAG ACGGTGAACT GAAACAGTCC
    ACCGAGAAAC CCGAAGAGGC GCCCCAACGT GAGCCGAGCT GCGCGGAAAT CTCCCTTCAG
    ACCGAGTCTG GTCAAGCAGC TGAGGAAGGC AAAGATGAAG GAGAAGAGAA ACAAGAGAAA
    GAAAGCGCCA GACCTGCAGA CTCCCCGACT AGTCCCGTGG CCAGCGAAAC CGCCTCGCCC
    TTCAGAAAAT TCTTCACTCA AGGCTGGGCC GGCTGGCGCA AAAAGACCAG CTTCAGGAAG
    CCTCGGGAGG AGGAGCTGGA GGCTTCAGAG AAGAAAAAGG AACAGGAGCC AGAAAAAGCC
    GAAGAAAATG AGAAGATGGA AGAGCCCTGC GAGCAGCCAC CTGCCCAGGA GGCTCCTGAG
    AGCACCCAGG ATGCCAGGCT GTCGGCCGAG TATGAAAAAG TGGAGCTGCC CCCTGAAGAC
    CAAGTGCAGG CACCTCCCGA AGAGAAACCA GCCCCGTTAG CAACCGAAGT GTTTGATGAA
    AAGGTCGAGA TTGTCGCCGA AGTCCACGTT AGCACTGTAG AGAAGGACCA GGAAGAGCCG
    AAAGCCGAAG CGGAAGAAAC AGTGGAGCTC TCGCCGCCCG AAAAGTCGAC CGAGACGAGC
    GCCGACCTTC AGGAAGCCGA GTCGGCCGAG ATGCTGAAGA CAGAGGAAGA TGCGGGTGCC
    CTGAGCGGGG ACCACTCCCA GGCAACCGAG CTCAGCGGGG ACGCGAAATC GCCGTCCACG
    CCCCCCGAGG GCACCGTGAG GGAGGCCGAA ATGCTGTCCT CGCAGGAGAG AATTAAGGTG
    CAGGGAAGCC CTTTGAAGAA ACTATTCACG AGCACCGGCT TGAAAAAGCT CTCTGGAAAG
    AAACAGAAAG GGAAACGAGG CGGCGACGAG GAGTCCGGGG AGCAGCCCTC AGCCTCCATG
    GATTCTCCCG ACAGTCCAGA CGAACTGAAG GGCGTTGAGA GCTCAGCCTC GTCTCCAGAA
    GAGCCCGAGG AGATCACCTG CCTGGAGAAA GGGGTGGCGG AGGCCCCGGG ACAGGATGGG
    GAGGCGGAGG AAGGGACCAC TTCCGACGGG GAGAAGAAGA GGGAAGGTGG TGTCACTCCC
    TGGGCATCTT TCAAAAAGAT GGTGACGCCC AAGAAACGCG TGCGAAGGCT CTCTGAAAGC
    GATAAGGAGG ACGAGGCAGA CAAAGTCAAA AGCGCCACCC TGTCCTCCAC AGAGAGCGCG
    GCCTCCGAAG TACAGGAAGA GGCCAAAGGG AACGGAGAGG AGCCGAAGCC CGAGGAGCCC
    AAGCGCAAGG TCGACACCTC CGTGTCCTGG GAAGCGCTGA TTTGCGTGGG GTCATCCAAG
    AAGAGAGCCA GAAAAGCATC CTCTTCCGAC GATGAAGCAG GGCCGAAACC TCTTGGAGGG
    GACAGCCAGA AAGCAGAGGA AGCTGGGAGG GACAAGGAGC CGGGCCCGGA GGCAGCCGCT
    GCCGGTTCCC AGGACCACGA GCAACCGCCG GGAAGCTCCT CACCCGAGCC GGCTGGCAGC
    CCATCCGAAG GGGAGGGCGT CTCCACCTGG GAAACCTTTA AAAGACTGGT CACCTCGAGA
    AAAAAATCGA AGTCAAAACT GGAAGAGAGA AGCGAAGACT CTGTAGCTGG GTCTGGCGCA
    GAACACGCAG CCTCAGAGGC TGAGCCTGGG AAAGAAGAGT CCTGGGTTTC CATCCGGAAG
    TTTATTCCTG GGCGAAGGAA GAAAAGGTCA GATGGGAAGC AGGAGCCAGC CGCTGTCGAA
    GAGGCGGGGC CAGCGGAGGC TAACGAGGAC GATGCGGACA TCCCAGCCGT GGTCCCTCTG
    TCTGAATACG ACGCAGTGGA AAGAGAGAAA ACCGAAGCCC AGCCAGCCCC CAAGAGCCAG
    GAGGGGCCCG AGCAGAAGCA GGTGGATGTC GACGTGTCGG AGGAGCTCAG TAAGAGCCTG
    GTTCACACCG TGTCGGTGGC TGTCGTGGAT GGGACAAGGG CCATTACCAA CATCGAAGAA
    AGGGCACCCT CCTGGATCTC AGCTTCGGTG ACAGAACCGC TGGAACAAGC CGAAGGTGAA
    GCCACACCAC CAAGCGAGGA GGTGCTTGAA AGAGAAGTCG TTGCGGAGGA AACCCCCATC
    GTTACCAAAG CGCTGCCAGA GAGCCAGGAG GTGGGTGATG ACACGATGGC CAGCGAGGTG
    GAATTAACCT CAGAAGCTGT GACAGCCGCT GAAACCACAG AGGCCTCTGG TGCGGAAGAG
    ACCACCGACA TGGTTTCGGC TGTCTCTCAG TTAACCGACT CTCCAGACAC CACCGAGGAA
    CCGACACCAG TGCAAGAGGT GGAGGGAGGC GTGCCTGACG CGGAGGACCA GGCGAGAAGG
    ACCCGAGAGG TGCTGCAGGC TGTTGCAGAA AAAGTTAGAG AAGAGTCACA GCTGCCAGAT
    GACGCCATCC AGACAACCCA GAAAGGACAA GCAAAAATAC TGGAGAAAGT GGAAGAGGCC
    GAGGAGGGAT CTCAGGCCCT AGATCTGAAG GAAGTGATGG ATGGAGTATC CAAAGTGCTT
    GTCCAAGATA CTGAAACTGA GACTTTGACA CAGGAGAAGG TGGTTGCAGA GGCCACCGCA
    GAAAGCTTGG GAGAAGTTCC TCCAGGCACA GACAGTGCAG AGGCCAGGGA GCTGGCGAGC
    ACTTGTCTTA TTGAAACCGG GACTGGGGTA GAAGCAGAGA CTGCCCCAGA GCAGGCTGTT
    GCTCCTGACT CAACTGAAAC CCTCACGGAC AGTGAGACCA ATGGAAGCAC CCCAGTAGCA
    GATCTTGACG CTTCAAATCT AAGCCAGCCA GACACGATCA TGGATGTCCA TGAAGATGGT
    GAGGTTCCAG CCGGCACTCA GTCCCAGGTC CCCGAAGGCG AGGTGCCTCC TGCAGTGAAA
    GAGCTGCCTG CAGCATCTTC TGATTTTCAG TTGCAGGAGG AAAGGTCTTC AAAGATGGCA
    GAGGGTCTAG AACACACGGA TGAAGAGGAG GGAACAGTGG AGACTGTAGC CATCCTTTCA
    AAGACTGAGA TGATTCAAGA GACTGGCCAG TGCTTTGATG AGGAAGCCAA AGAAGAACCA
    TCCATGGAAG GACTTGCCTT GTCTGCCGAC ACAGAAATAA CTGAGAAAAA GATAACTGAA
    GTTGTCCTTG AGGAGGACGT TACTAAGAAA GTTGAATTTC AAGAGAATAA TATAGAACTC
    CAGAGTCCTG CTAAGTCTCT TCCAACCCCA GCAGAGAGAG AGATGGTAGT TGAAGGGAAA
    AGGGAAACAG TGGAAGTGGA GGCAACTGAA GGGAATGAAG AGAAACTTGA GCACGAACCA
    GCTGTGGCCG TATGTGAAGA GCTCAGTAAG CAACTGGTTC AGACAGTGAA TGTCACCATC
    ATCGATGGGG AAAAGGAAGT CGTCAGTTTT GAAGGAAGTT CTCCCCTGCT GGCTTCCGAG
    GAAGAGGCAT GCACGGGAAT CTCAGTTCAA AGCTCTGAGG CAGCATTAGC TCTAATAGCT
    GCAGCCGTGG AGGAGAAGGT CTTAGGAGAA GCTATCAAGA TTGTAGAAAC AACCGAGGCT
    TTGAAATCTG CAGAAGCTCA TCTAGTACTG GAAGAAGAGT CCTCAGAAAA AGACGAAGAG
    TTTCCTGCCC AGCCTGGGGA AGAAGATGTG GCATCCACAG GGACTGAGTC TCAAGCAGAA
    TCAATACCGA CGATAGTATC TATTACGCCT GGAAAAGGCA TCGACGCTGA CCTGGAAGGA
    GATGAAACCA CATCACAGAA ACAGGAGTTG GGTGGAGACG GTGAGCAGGT TGGTGGTCAG
    GAAGGCAGAG AGAGCAACAC AGGAGAGGAA GACGGCAAGG CGGAAAGTGA GATTTCGAAA
    CTTGAGAAGG AGAGCTGCAG ACTTGTAGAG AGCGTGATTC AGACAGCCGT CGACCGTTTG
    GGGAGTACAG AAGAAACGGC CACCGATGTC CAGACCCAGG CTCAACTGGC AGAAGCCGAC
    AGCCAGGAAG CTGGGCAGAA AATGGAGAAA GAAGAAAGCA AACCTCAGGC CTGCCTGGTG
    GATGAAACAC AAGCCGTAGA AGCCAAAGAG GCCAAAGAAG CCAAAGAGGA GTCCCCACTA
    AGCACCGGCG AACATGCGCA TCTCAGTGTT TCCAAAGATG CGAATGAAGC TTCAGAGAAG
    GTGGCAGCCA CTAGCATAGA AGGTTCCAGG GTAGACGACC AGCGGCTTGA AGAGGTAGCT
    CTCCCATCCC AGAAAAAGAG AGAAATCCTT GAAACAGAGT CTGTGCTGCA AGATGACGGC
    AGTGCCGGGT TTGGAGAAAG AAAGAAGTCC CCATCTGAAT CCCGAGAGGA TGAAAAAGGT
    GATGCTGCCG GTGACCCTGG AAACCAAACC TCAATCCCAG AGGATGCTGA GGCCTCGGGA
    GGCTCAACCA AAGAGTCCCT AGATACAAAT GGACCCAAAC TGAAAGAGAA GGGAGACAGC
    CAGGAGGAAA AGGTGCAGAG CGAGTCGGAA AAAGAGATGA AAACACAAAC ACAGGAGGAG
    ACACAGAACC AAGAGAGAGA TCTGGCAAAA CCCGAACCCA CGGAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.