FAM21A cDNA ORF clone, Bos taurus(cattle)

The following FAM21A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAM21A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239156 NM_001205767.2
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa88966 XM_015461061.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa88967 XM_015461062.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa88968 XM_015461063.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa88969 XM_015461064.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa88970 XM_015461065.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa88971 XM_015461066.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa88972 XM_015461067.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa34349 XM_005226575.3
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa38695 NM_001205767.1
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa34346 XM_005226572.3
Latest version!
Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239156
    Clone ID Related Accession (Same CDS sequence) NM_001205767.2
    Accession Version NM_001205767.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-17
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151722.1 and NKLS02000028.1. On Jun 18, 2018 this sequence version replaced NM_001205767.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.1188.1, SRR6764281.29330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTTGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGATGA CGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA ATCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192696.1
    CDS103..4104
    Translation

    Target ORF information:

    RefSeq Version NM_001205767.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTTGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGATGA CGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA ATCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88966
    Clone ID Related Accession (Same CDS sequence) XM_015461061.1
    Accession Version XM_015461061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316547.1
    CDS108..4169
    Translation

    Target ORF information:

    RefSeq Version XM_015461061.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88967
    Clone ID Related Accession (Same CDS sequence) XM_015461062.1
    Accession Version XM_015461062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG TCAGGAAGCC CACCCACAGT
    GGCTCGCAGC TGGTCTGTCC TGTCCTTCCA CCCACAGGGG ATACTGATGT GTTTGGTGCC
    ACCTCTGCCC CGGCACCGAG GGAGCCCCAG AAGCACGAGC GGCCCCCTCC AGGGAAAAGC
    GCGTACGTCC CAGCGTCGGC TGGCCTCTTC GATGATGACG ACGACGATGA TGACTTTTTC
    TCGGCATCCC GTAGCAAACC TCCCAAGACA GACAAAGTCG AGTCCACTGC CGGTATCTTT
    GACGATGAAG AAGGAGATCT GTTCAAAGAA AGAACAGTGG CTTTGCCAGA AGCTACTGTG
    AATCAGACAG ATGGAAACAA AGCAAGAGCA GAAACAAAGG TTATGCTACC TTCCAGCAAA
    AATCTCAAGC TCTCGTCGGA AACAAAGACT CACAAAGGTT TATTTTCAGA TGAGGAGGAC
    TCTGAGGATT TGTTTTCTTC TCAAAGTGTG AGTAAGTCAA AAGATGCAAG TGTCCTGCCT
    AGCAAACACC GCAGCTCAGT CTTGCTTTTT GATGATGAAG ATGGACAGGA TAATCTTTTT
    GATACTCCAG CTGCTAAGAA GCAGACATCG TCTCTACCGA CTCAGAGCCA AAAGAAAACA
    AAGCCCTCTG AGCCTCCCAA AAAGAAAGCA TCTGCCTTAC TGTTCAGCAG TGATGAGGAG
    GACCAGTGGC ATGTTACTGA CTCACAGACC AACTCCACAT CTGACAAGTC TAAAGGAGAA
    CCTAGGGACC CTGAGCCCAC CCACCAGGAG GTTAAGGCCG TGAAAAAGAC CAGCCTGTTT
    GAGGAGGATG ACGAGGATGA CCTGTTTGCT ATTGCCAAGG ACAGCCAAAA GAAGGCCCAG
    AGAGCATCAC TTCTGTTTGA AGATGATGCT GACAGCGGAA GCTCTCTGTT CGGCTCTCCT
    CCCACATCTG TTCCTCCTGC AACAACGAAA AAAGAGACTG TCCCTGAAGT ACCGCCTTTG
    CTGTTCAGCG ATGAGGAAGA AAAGGCGGCT CAGTCTGGAG TGAAACCTGC AGCTAAGAAG
    GCTGGGAGCG CCAAGGGGAC CTCAGAACTT GGGAGAACTC CTGCGGTCGA GGAGTCAGGA
    AAGGAAGGAT CTTTGAATGT ACCTACTCAG GAAACAGCCA AGAACTCTGA TTTATTTTCT
    TCATCATCCC CATTGGACAA AGGAACTAAA AGTAGAACCA AAACGGTTCT TAGCTTATTT
    GATGAGGAAG AGGATAAAAC GGAAGATCAA AGCAGCTTCC AAGCTCTGAG AAAAGAAGTA
    GGAAAGAGTC CTGATCCTGG TACCCGCCCC AAGAGCACAG GTGTCTTTCA GGATGAAGAG
    CTCCTTTTTA GTCACAAGCT CCAAAAGGAC AACGACCCAG ATGTTGACCT TTTCTCTGGC
    ACCAAAAAAT CCAGGTTGTT AGAGCCCAGT GTTGGGAGCC TGTTTGGAGA TGATGAAGAC
    GATGATCTTT TCAGCTCTGC CAAGTCGCAG CCTTTGATCC CAGAAAAGAA GAAGGTAGTG
    AAAAAAGACA ACTCTATTTC CTCTGTCAAG AACCAGAAAC ATCCTGAGTC CACTCAGGGT
    ATCAAAGAAA AAGACGTATG GAAATCGGAA ACACCTCAGG ACTCACCAGG TCCCGCTCCA
    CTTAAAACCA AAGAGCCATC CCCTCGGATC TGGAAAATAC AAGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316548.1
    CDS108..4166
    Translation

    Target ORF information:

    RefSeq Version XM_015461062.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG TCAGGAAGCC CACCCACAGT
    GGCTCGCAGC TGGTCTGTCC TGTCCTTCCA CCCACAGGGG ATACTGATGT GTTTGGTGCC
    ACCTCTGCCC CGGCACCGAG GGAGCCCCAG AAGCACGAGC GGCCCCCTCC AGGGAAAAGC
    GCGTACGTCC CAGCGTCGGC TGGCCTCTTC GATGATGACG ACGACGATGA TGACTTTTTC
    TCGGCATCCC GTAGCAAACC TCCCAAGACA GACAAAGTCG AGTCCACTGC CGGTATCTTT
    GACGATGAAG AAGGAGATCT GTTCAAAGAA AGAACAGTGG CTTTGCCAGA AGCTACTGTG
    AATCAGACAG ATGGAAACAA AGCAAGAGCA GAAACAAAGG TTATGCTACC TTCCAGCAAA
    AATCTCAAGC TCTCGTCGGA AACAAAGACT CACAAAGGTT TATTTTCAGA TGAGGAGGAC
    TCTGAGGATT TGTTTTCTTC TCAAAGTGTG AGTAAGTCAA AAGATGCAAG TGTCCTGCCT
    AGCAAACACC GCAGCTCAGT CTTGCTTTTT GATGATGAAG ATGGACAGGA TAATCTTTTT
    GATACTCCAG CTGCTAAGAA GCAGACATCG TCTCTACCGA CTCAGAGCCA AAAGAAAACA
    AAGCCCTCTG AGCCTCCCAA AAAGAAAGCA TCTGCCTTAC TGTTCAGCAG TGATGAGGAG
    GACCAGTGGC ATGTTACTGA CTCACAGACC AACTCCACAT CTGACAAGTC TAAAGGAGAA
    CCTAGGGACC CTGAGCCCAC CCACCAGGAG GTTAAGGCCG TGAAAAAGAC CAGCCTGTTT
    GAGGAGGATG ACGAGGATGA CCTGTTTGCT ATTGCCAAGG ACAGCCAAAA GAAGGCCCAG
    AGAGCATCAC TTCTGTTTGA AGATGATGCT GACAGCGGAA GCTCTCTGTT CGGCTCTCCT
    CCCACATCTG TTCCTCCTGC AACAACGAAA AAAGAGACTG TCCCTGAAGT ACCGCCTTTG
    CTGTTCAGCG ATGAGGAAGA AAAGGCGGCT CAGTCTGGAG TGAAACCTGC AGCTAAGAAG
    GCTGGGAGCG CCAAGGGGAC CTCAGAACTT GGGAGAACTC CTGCGGTCGA GGAGTCAGGA
    AAGGAAGGAT CTTTGAATGT ACCTACTCAG GAAACAGCCA AGAACTCTGA TTTATTTTCT
    TCATCATCCC CATTGGACAA AGGAACTAAA AGTAGAACCA AAACGGTTCT TAGCTTATTT
    GATGAGGAAG AGGATAAAAC GGAAGATCAA AGCAGCTTCC AAGCTCTGAG AAAAGAAGTA
    GGAAAGAGTC CTGATCCTGG TACCCGCCCC AAGAGCACAG GTGTCTTTCA GGATGAAGAG
    CTCCTTTTTA GTCACAAGCT CCAAAAGGAC AACGACCCAG ATGTTGACCT TTTCTCTGGC
    ACCAAAAAAT CCAGGTTGTT AGAGCCCAGT GTTGGGAGCC TGTTTGGAGA TGATGAAGAC
    GATGATCTTT TCAGCTCTGC CAAGTCGCAG CCTTTGATCC CAGAAAAGAA GAAGGTAGTG
    AAAAAAGACA ACTCTATTTC CTCTGTCAAG AACCAGAAAC ATCCTGAGTC CACTCAGGGT
    ATCAAAGAAA AAGACGTATG GAAATCGGAA ACACCTCAGG ACTCACCAGG TCCCGCTCCA
    CTTAAAACCA AAGAGCCATC CCCTCGGATC TGGAAAATAC AAGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88968
    Clone ID Related Accession (Same CDS sequence) XM_015461063.1
    Accession Version XM_015461063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGGTTCAGTA GGCAGTGATC GTGGGAGTAT CGCAGACAGT
    GAAGAAGAGA ACGAGGAAGA GGAGTCAGAT GAAGATTTTG CCAATCATAG TGACAATGAT
    CAAAACCGGC ACATTGCAAC GATGAGTGAT GAAGAAGAGG ATGATGATGG TTGCGACATT
    TTTGCTGACT CTGAGAAGGA GGAGGAAGAT GTTGAAGACA TGGAAGAAAA CTCTAGGCCT
    AAAAGAAGCA GGCCTACATC ATTTGCTGAT GAACTGGCAG CTCGGATCAA AGGGGACGCC
    TCGAGCAGGA TGGAAGGGGA GCGCACAGCC TTATCCCCAG GGGAAGCAAA ACCTCAGAAG
    ACGTTGAAAG AGAAGAAGGA AAGGAAAACT CCCTCAGATG ATGAAGAAGA TAACTTATTC
    GCACCCCCGA AGCTGACAGA TGAAGACTTC TCGCCGTTTG GTTCTAGAGG CGGCCTGTTC
    AGTGGAGGGA AGGGACTTTT TGATGATGAG GATGAGGAGC AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG TCAGGAAGCC CACCCACAGT
    GGCTCGCAGC TGGTCTGTCC TGTCCTTCCA CCCACAGGGG ATACTGATGT GTTTGGTGCC
    ACCTCTGCCC CGGCACCGAG GGAGCCCCAG AAGCACGAGC GGCCCCCTCC AGGGAAAAGC
    GCGTACGTCC CAGCGTCGGC TGGCCTCTTC GATGATGACG ACGACGATGA TGACTTTTTC
    TCGGCATCCC GTAGCAAACC TCCCAAGACA GACAAAGTCG AGTCCACTGC CGGTATCTTT
    GACGATGAAG AAGGAGATCT GTTCAAAGAA AGAACAGTGG CTTTGCCAGA AGCTACTGTG
    AATCAGACAG ATGGAAACAA AGCAAGAGCA GAAACAAAGG TTATGCTACC TTCCAGCAAA
    AATCTCAAGC TCTCGTCGGA AACAAAGACT CACAAAGGTT TATTTTCAGA TGAGGAGGAC
    TCTGAGGATT TGTTTTCTTC TCAAAGTGTG AGTAAGTCAA AAGATGCAAG TGTCCTGCCT
    AGCAAACACC GCAGCTCAGT CTTGCTTTTT GATGATGAAG ATGGACAGGA TAATCTTTTT
    GATACTCCAG CTGCTAAGAA GCAGACATCG TCTCTACCGA CTCAGAGCCA AAAGAAAACA
    AAGCCCTCTG AGCCTCCCAA AAAGAAAGCA TCTGCCTTAC TGTTCAGCAG TGATGAGGAG
    GACCAGTGGC ATGTTACTGA CTCACAGACC AACTCCACAT CTGACAAGTC TAAAGGAGAA
    CCTAGGGACC CTGAGCCCAC CCACCAGGAG GTTAAGGCCG TGAAAAAGAC CAGCCTGTTT
    GAGGAGGATG ACGAGGATGA CCTGTTTGCT ATTGCCAAGG ACAGCCAAAA GAAGGCCCAG
    AGAGCATCAC TTCTGTTTGA AGATGATGCT GACAGCGGAA GCTCTCTGTT CGGCTCTCCT
    CCCACATCTG TTCCTCCTGC AACAACGAAA AAAGAGACTG TCCCTGAAGT ACCGCCTTTG
    CTGTTCAGCG ATGAGGAAGA AAAGGCGGCT CAGTCTGGAG TGAAACCTGC AGCTAAGAAG
    GCTGGGAGCG CCAAGGGGAC CTCAGAACTT GGGAGAACTC CTGCGGTCGA GGAGTCAGGA
    AAGGAAGGAT CTTTGAATGT ACCTACTCAG GAAACAGCCA AGAACTCTGA TTTATTTTCT
    TCATCATCCC CATTGGACAA AGGAACTAAA AGTAGAACCA AAACGGTTCT TAGCTTATTT
    GATGAGGAAG AGGATAAAAC GGAAGATCAA AGCAGCTTCC AAGCTCTGAG AAAAGAAGTA
    GGAAAGAGTC CTGATCCTGG TACCCGCCCC AAGAGCACAG GTGTCTTTCA GGATGAAGAG
    CTCCTTTTTA GTCACAAGCT CCAAAAGGAC AACGACCCAG ATGTTGACCT TTTCTCTGGC
    ACCAAAAAAT CCAGGTTGTT AGAGCCCAGT GTTGGGAGCC TGTTTGGAGA TGATGAAGAC
    GATGATCTTT TCAGCTCTGC CAAGTCGCAG CCTTTGATCC CAGAAAAGAA GAAGGTAGTG
    AAAAAAGACA ACTCTATTTC CTCTGTCAAG AACCAGAAAC ATCCTGAGTC CACTCAGGGT
    ATCAAAGAAA AAGACGTATG GAAATCGGAA ACACCTCAGG ACTCACCAGG TCCCGCTCCA
    CTTAAAACCA AAGAGCCATC CCCTCGGATC TGGAAAATAC AAGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316549.1
    CDS108..4166
    Translation

    Target ORF information:

    RefSeq Version XM_015461063.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGGTTCAGTA GGCAGTGATC GTGGGAGTAT CGCAGACAGT
    GAAGAAGAGA ACGAGGAAGA GGAGTCAGAT GAAGATTTTG CCAATCATAG TGACAATGAT
    CAAAACCGGC ACATTGCAAC GATGAGTGAT GAAGAAGAGG ATGATGATGG TTGCGACATT
    TTTGCTGACT CTGAGAAGGA GGAGGAAGAT GTTGAAGACA TGGAAGAAAA CTCTAGGCCT
    AAAAGAAGCA GGCCTACATC ATTTGCTGAT GAACTGGCAG CTCGGATCAA AGGGGACGCC
    TCGAGCAGGA TGGAAGGGGA GCGCACAGCC TTATCCCCAG GGGAAGCAAA ACCTCAGAAG
    ACGTTGAAAG AGAAGAAGGA AAGGAAAACT CCCTCAGATG ATGAAGAAGA TAACTTATTC
    GCACCCCCGA AGCTGACAGA TGAAGACTTC TCGCCGTTTG GTTCTAGAGG CGGCCTGTTC
    AGTGGAGGGA AGGGACTTTT TGATGATGAG GATGAGGAGC AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG TCAGGAAGCC CACCCACAGT
    GGCTCGCAGC TGGTCTGTCC TGTCCTTCCA CCCACAGGGG ATACTGATGT GTTTGGTGCC
    ACCTCTGCCC CGGCACCGAG GGAGCCCCAG AAGCACGAGC GGCCCCCTCC AGGGAAAAGC
    GCGTACGTCC CAGCGTCGGC TGGCCTCTTC GATGATGACG ACGACGATGA TGACTTTTTC
    TCGGCATCCC GTAGCAAACC TCCCAAGACA GACAAAGTCG AGTCCACTGC CGGTATCTTT
    GACGATGAAG AAGGAGATCT GTTCAAAGAA AGAACAGTGG CTTTGCCAGA AGCTACTGTG
    AATCAGACAG ATGGAAACAA AGCAAGAGCA GAAACAAAGG TTATGCTACC TTCCAGCAAA
    AATCTCAAGC TCTCGTCGGA AACAAAGACT CACAAAGGTT TATTTTCAGA TGAGGAGGAC
    TCTGAGGATT TGTTTTCTTC TCAAAGTGTG AGTAAGTCAA AAGATGCAAG TGTCCTGCCT
    AGCAAACACC GCAGCTCAGT CTTGCTTTTT GATGATGAAG ATGGACAGGA TAATCTTTTT
    GATACTCCAG CTGCTAAGAA GCAGACATCG TCTCTACCGA CTCAGAGCCA AAAGAAAACA
    AAGCCCTCTG AGCCTCCCAA AAAGAAAGCA TCTGCCTTAC TGTTCAGCAG TGATGAGGAG
    GACCAGTGGC ATGTTACTGA CTCACAGACC AACTCCACAT CTGACAAGTC TAAAGGAGAA
    CCTAGGGACC CTGAGCCCAC CCACCAGGAG GTTAAGGCCG TGAAAAAGAC CAGCCTGTTT
    GAGGAGGATG ACGAGGATGA CCTGTTTGCT ATTGCCAAGG ACAGCCAAAA GAAGGCCCAG
    AGAGCATCAC TTCTGTTTGA AGATGATGCT GACAGCGGAA GCTCTCTGTT CGGCTCTCCT
    CCCACATCTG TTCCTCCTGC AACAACGAAA AAAGAGACTG TCCCTGAAGT ACCGCCTTTG
    CTGTTCAGCG ATGAGGAAGA AAAGGCGGCT CAGTCTGGAG TGAAACCTGC AGCTAAGAAG
    GCTGGGAGCG CCAAGGGGAC CTCAGAACTT GGGAGAACTC CTGCGGTCGA GGAGTCAGGA
    AAGGAAGGAT CTTTGAATGT ACCTACTCAG GAAACAGCCA AGAACTCTGA TTTATTTTCT
    TCATCATCCC CATTGGACAA AGGAACTAAA AGTAGAACCA AAACGGTTCT TAGCTTATTT
    GATGAGGAAG AGGATAAAAC GGAAGATCAA AGCAGCTTCC AAGCTCTGAG AAAAGAAGTA
    GGAAAGAGTC CTGATCCTGG TACCCGCCCC AAGAGCACAG GTGTCTTTCA GGATGAAGAG
    CTCCTTTTTA GTCACAAGCT CCAAAAGGAC AACGACCCAG ATGTTGACCT TTTCTCTGGC
    ACCAAAAAAT CCAGGTTGTT AGAGCCCAGT GTTGGGAGCC TGTTTGGAGA TGATGAAGAC
    GATGATCTTT TCAGCTCTGC CAAGTCGCAG CCTTTGATCC CAGAAAAGAA GAAGGTAGTG
    AAAAAAGACA ACTCTATTTC CTCTGTCAAG AACCAGAAAC ATCCTGAGTC CACTCAGGGT
    ATCAAAGAAA AAGACGTATG GAAATCGGAA ACACCTCAGG ACTCACCAGG TCCCGCTCCA
    CTTAAAACCA AAGAGCCATC CCCTCGGATC TGGAAAATAC AAGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88969
    Clone ID Related Accession (Same CDS sequence) XM_015461064.1
    Accession Version XM_015461064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316550.1
    CDS108..4106
    Translation

    Target ORF information:

    RefSeq Version XM_015461064.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88970
    Clone ID Related Accession (Same CDS sequence) XM_015461065.1
    Accession Version XM_015461065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGT TGTTAGAGCC CAGTGTTGGG AGCCTGTTTG GAGATGATGA AGACGATGAT
    CTTTTCAGCT CTGCCAAGTC GCAGCCTTTG ATCCCAGAAA AGAAGAAGGT AGTGAAAAAA
    GACAACTCTA TTTCCTCTGT CAAGAACCAG AAACATCCTG AGTCCACTCA GGGTATCAAA
    GAAAAAGACG TATGGAAATC GGAAACACCT CAGGACTCAC CAGGTCCCGC TCCACTTAAA
    ACCAAAGAGC CATCCCCTCG GATCTGGAAA ATACAAGCAA ATTTAGCGAT CAACCCAGCA
    GCCTTGCTGC CTCCAGCAGC TCCCCAGATC TCTGGAATGA AGTCTGTTTT GCCAGAATCT
    GGTGCTCCTT CGTCGGAACC TGGGAGGATG CAGAGTCTGG ACCGTGTGCC CACTCCTCCT
    GGGAGTGGGG AGGCTGGTGT GAGCTTTGAC CTTCCAGCTC AGGCAGATAC CTTACACTGT
    GCGAACAAGA CCCGGGTCAA AGTGAGAGGG AAGCGCAGAC CGCAGACGCG CGCAGCTCGA
    CACCTGGCTG CCCAGGAGTC CACTGAGGCC GAGGATGTGG GTTCCCCCAG GGGATTTGTG
    GCACAGTTGA CAGATGGTGC CACATCACCA GATGGTCGTC AGCCACCGCT GAGGGCAGCT
    GGTGGAGCAG AAGAAGCAGT GGCAGCTGCC ACTCCAACAT GGGCAGGTGG TCCTGTGCCT
    GGAATGAACA GAGGCCCATT TGTGAAATCT GTGGGTCAAC CTCTTGAGGA TGATCTGTTT
    GATTCTGGAG ATATCTTTTC CAAGGGCATT GCATCTCAGT CCGCAGGAAG AAAAGCCAAG
    GTGAAGGCAG CCAGCAGCCT GGCCAACCTG GCAGAGGGAA GTAAAGACAG AAGCCCCATG
    TTCCCTGCTC TCGGTGAGAC AGGCAGTGAT GATGATCTCT TTCAGTCCAT TAAACCAAAA
    CCAGCAAAGA AAACAAATCC TTTCCCTTTC CTGGAAGATG AGGACGATCT GTTTACAGAC
    CAGAGAGGCA AGAAGAATGG GTCAAAATCC AACAGTCAAC AGGATATTAT CTCAAAAGCA
    CAAGATATAT TTGAGGATGA TATATTTGCT ACAGAAGCAA ATAAACCCTC ACAAAAAACA
    AGAGAGAAGG AAAGAACATT TGACTCCAAC TTGTTCGATG ACAACATTGA TATCTTTGCT
    GACCTAACTG TAAAACCAAA AGAAAAGTCC AAAAAGAAAG TGGAAGCTAA GTCTATATTT
    GACGATGATA CAGATGATAT CTTCTCCTCT GGTATCCAGA CTAAGACAAC CAAATCAAAA
    AGTCGATCTT CACAGAAAGT GACTGAATCG AGATCTGGAC AGAAGGTTTC CAACATATTT
    GATGATCCCT TGAATGCCTT TGGAGGCCAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316551.1
    CDS108..4040
    Translation

    Target ORF information:

    RefSeq Version XM_015461065.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGT TGTTAGAGCC CAGTGTTGGG AGCCTGTTTG GAGATGATGA AGACGATGAT
    CTTTTCAGCT CTGCCAAGTC GCAGCCTTTG ATCCCAGAAA AGAAGAAGGT AGTGAAAAAA
    GACAACTCTA TTTCCTCTGT CAAGAACCAG AAACATCCTG AGTCCACTCA GGGTATCAAA
    GAAAAAGACG TATGGAAATC GGAAACACCT CAGGACTCAC CAGGTCCCGC TCCACTTAAA
    ACCAAAGAGC CATCCCCTCG GATCTGGAAA ATACAAGCAA ATTTAGCGAT CAACCCAGCA
    GCCTTGCTGC CTCCAGCAGC TCCCCAGATC TCTGGAATGA AGTCTGTTTT GCCAGAATCT
    GGTGCTCCTT CGTCGGAACC TGGGAGGATG CAGAGTCTGG ACCGTGTGCC CACTCCTCCT
    GGGAGTGGGG AGGCTGGTGT GAGCTTTGAC CTTCCAGCTC AGGCAGATAC CTTACACTGT
    GCGAACAAGA CCCGGGTCAA AGTGAGAGGG AAGCGCAGAC CGCAGACGCG CGCAGCTCGA
    CACCTGGCTG CCCAGGAGTC CACTGAGGCC GAGGATGTGG GTTCCCCCAG GGGATTTGTG
    GCACAGTTGA CAGATGGTGC CACATCACCA GATGGTCGTC AGCCACCGCT GAGGGCAGCT
    GGTGGAGCAG AAGAAGCAGT GGCAGCTGCC ACTCCAACAT GGGCAGGTGG TCCTGTGCCT
    GGAATGAACA GAGGCCCATT TGTGAAATCT GTGGGTCAAC CTCTTGAGGA TGATCTGTTT
    GATTCTGGAG ATATCTTTTC CAAGGGCATT GCATCTCAGT CCGCAGGAAG AAAAGCCAAG
    GTGAAGGCAG CCAGCAGCCT GGCCAACCTG GCAGAGGGAA GTAAAGACAG AAGCCCCATG
    TTCCCTGCTC TCGGTGAGAC AGGCAGTGAT GATGATCTCT TTCAGTCCAT TAAACCAAAA
    CCAGCAAAGA AAACAAATCC TTTCCCTTTC CTGGAAGATG AGGACGATCT GTTTACAGAC
    CAGAGAGGCA AGAAGAATGG GTCAAAATCC AACAGTCAAC AGGATATTAT CTCAAAAGCA
    CAAGATATAT TTGAGGATGA TATATTTGCT ACAGAAGCAA ATAAACCCTC ACAAAAAACA
    AGAGAGAAGG AAAGAACATT TGACTCCAAC TTGTTCGATG ACAACATTGA TATCTTTGCT
    GACCTAACTG TAAAACCAAA AGAAAAGTCC AAAAAGAAAG TGGAAGCTAA GTCTATATTT
    GACGATGATA CAGATGATAT CTTCTCCTCT GGTATCCAGA CTAAGACAAC CAAATCAAAA
    AGTCGATCTT CACAGAAAGT GACTGAATCG AGATCTGGAC AGAAGGTTTC CAACATATTT
    GATGATCCCT TGAATGCCTT TGGAGGCCAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88971
    Clone ID Related Accession (Same CDS sequence) XM_015461066.1
    Accession Version XM_015461066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGT TGTTAGAGCC CAGTGTTGGG AGCCTGTTTG GAGATGATGA AGACGATGAT
    CTTTTCAGCT CTGCCAAGTC GCAGCCTTTG ATCCCAGAAA AGAAGAAGGT AGTGAAAAAA
    GACAACTCTA TTTCCTCTGT CAAGAACCAG AAACATCCTG AGTCCACTCA GGGTATCAAA
    GAAAAAGACG TATGGAAATC GGAAACACCT CAGGCAAATT TAGCGATCAA CCCAGCAGCC
    TTGCTGCCTC CAGCAGCTCC CCAGATCTCT GGAATGAAGT CTGTTTTGCC AGAATCTGGT
    GCTCCTTCGT CGGAACCTGG GAGGATGCAG AGTCTGGACC GTGTGCCCAC TCCTCCTGGG
    AGTGGGGAGG CTGGTGTGAG CTTTGACCTT CCAGCTCAGG CAGATACCTT ACACTGTGCG
    AACAAGACCC GGGTCAAAGT GAGAGGGAAG CGCAGACCGC AGACGCGCGC AGCTCGACAC
    CTGGCTGCCC AGGAGTCCAC TGAGGCCGAG GATGTGGGTT CCCCCAGGGG ATTTGTGGCA
    CAGTTGACAG ATGGTGCCAC ATCACCAGAT GGTCGTCAGC CACCGCTGAG GGCAGCTGGT
    GGAGCAGAAG AAGCAGTGGC AGCTGCCACT CCAACATGGG CAGGTGGTCC TGTGCCTGGA
    ATGAACAGAG GCCCATTTGT GAAATCTGTG GGTCAACCTC TTGAGGATGA TCTGTTTGAT
    TCTGGAGATA TCTTTTCCAA GGGCATTGCA TCTCAGTCCG CAGGAAGAAA AGCCAAGGTG
    AAGGCAGCCA GCAGCCTGGC CAACCTGGCA GAGGGAAGTA AAGACAGAAG CCCCATGTTC
    CCTGCTCTCG GTGAGACAGG CAGTGATGAT GATCTCTTTC AGTCCATTAA ACCAAAACCA
    GCAAAGAAAA CAAATCCTTT CCCTTTCCTG GAAGATGAGG ACGATCTGTT TACAGACCAG
    AGAGGCAAGA AGAATGGGTC AAAATCCAAC AGTCAACAGG ATATTATCTC AAAAGCACAA
    GATATATTTG AGGATGATAT ATTTGCTACA GAAGCAAATA AACCCTCACA AAAAACAAGA
    GAGAAGGAAA GAACATTTGA CTCCAACTTG TTCGATGACA ACATTGATAT CTTTGCTGAC
    CTAACTGTAA AACCAAAAGA AAAGTCCAAA AAGAAAGTGG AAGCTAAGTC TATATTTGAC
    GATGATACAG ATGATATCTT CTCCTCTGGT ATCCAGACTA AGACAACCAA ATCAAAAAGT
    CGATCTTCAC AGAAAGTGAC TGAATCGAGA TCTGGACAGA AGGTTTCCAA CATATTTGAT
    GATCCCTTGA ATGCCTTTGG AGGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316552.1
    CDS108..3977
    Translation

    Target ORF information:

    RefSeq Version XM_015461066.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGT TGTTAGAGCC CAGTGTTGGG AGCCTGTTTG GAGATGATGA AGACGATGAT
    CTTTTCAGCT CTGCCAAGTC GCAGCCTTTG ATCCCAGAAA AGAAGAAGGT AGTGAAAAAA
    GACAACTCTA TTTCCTCTGT CAAGAACCAG AAACATCCTG AGTCCACTCA GGGTATCAAA
    GAAAAAGACG TATGGAAATC GGAAACACCT CAGGCAAATT TAGCGATCAA CCCAGCAGCC
    TTGCTGCCTC CAGCAGCTCC CCAGATCTCT GGAATGAAGT CTGTTTTGCC AGAATCTGGT
    GCTCCTTCGT CGGAACCTGG GAGGATGCAG AGTCTGGACC GTGTGCCCAC TCCTCCTGGG
    AGTGGGGAGG CTGGTGTGAG CTTTGACCTT CCAGCTCAGG CAGATACCTT ACACTGTGCG
    AACAAGACCC GGGTCAAAGT GAGAGGGAAG CGCAGACCGC AGACGCGCGC AGCTCGACAC
    CTGGCTGCCC AGGAGTCCAC TGAGGCCGAG GATGTGGGTT CCCCCAGGGG ATTTGTGGCA
    CAGTTGACAG ATGGTGCCAC ATCACCAGAT GGTCGTCAGC CACCGCTGAG GGCAGCTGGT
    GGAGCAGAAG AAGCAGTGGC AGCTGCCACT CCAACATGGG CAGGTGGTCC TGTGCCTGGA
    ATGAACAGAG GCCCATTTGT GAAATCTGTG GGTCAACCTC TTGAGGATGA TCTGTTTGAT
    TCTGGAGATA TCTTTTCCAA GGGCATTGCA TCTCAGTCCG CAGGAAGAAA AGCCAAGGTG
    AAGGCAGCCA GCAGCCTGGC CAACCTGGCA GAGGGAAGTA AAGACAGAAG CCCCATGTTC
    CCTGCTCTCG GTGAGACAGG CAGTGATGAT GATCTCTTTC AGTCCATTAA ACCAAAACCA
    GCAAAGAAAA CAAATCCTTT CCCTTTCCTG GAAGATGAGG ACGATCTGTT TACAGACCAG
    AGAGGCAAGA AGAATGGGTC AAAATCCAAC AGTCAACAGG ATATTATCTC AAAAGCACAA
    GATATATTTG AGGATGATAT ATTTGCTACA GAAGCAAATA AACCCTCACA AAAAACAAGA
    GAGAAGGAAA GAACATTTGA CTCCAACTTG TTCGATGACA ACATTGATAT CTTTGCTGAC
    CTAACTGTAA AACCAAAAGA AAAGTCCAAA AAGAAAGTGG AAGCTAAGTC TATATTTGAC
    GATGATACAG ATGATATCTT CTCCTCTGGT ATCCAGACTA AGACAACCAA ATCAAAAAGT
    CGATCTTCAC AGAAAGTGAC TGAATCGAGA TCTGGACAGA AGGTTTCCAA CATATTTGAT
    GATCCCTTGA ATGCCTTTGG AGGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88972
    Clone ID Related Accession (Same CDS sequence) XM_015461067.1
    Accession Version XM_015461067.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGCAA ATTTAGCGAT CAACCCAGCA GCCTTGCTGC CTCCAGCAGC TCCCCAGATC
    TCTGGAATGA AGTCTGTTTT GCCAGAATCT GGTGCTCCTT CGTCGGAACC TGGGAGGATG
    CAGAGTCTGG ACCGTGTGCC CACTCCTCCT GGGAGTGGGG AGGCTGGTGT GAGCTTTGAC
    CTTCCAGCTC AGGCAGATAC CTTACACTGT GCGAACAAGA CCCGGGTCAA AGTGAGAGGG
    AAGCGCAGAC CGCAGACGCG CGCAGCTCGA CACCTGGCTG CCCAGGAGTC CACTGAGGCC
    GAGGATGTGG GTTCCCCCAG GGGATTTGTG GCACAGTTGA CAGATGGTGC CACATCACCA
    GATGGTCGTC AGCCACCGCT GAGGGCAGCT GGTGGAGCAG AAGAAGCAGT GGCAGCTGCC
    ACTCCAACAT GGGCAGGTGG TCCTGTGCCT GGAATGAACA GAGGCCCATT TGTGAAATCT
    GTGGGTCAAC CTCTTGAGGA TGATCTGTTT GATTCTGGAG ATATCTTTTC CAAGGGCATT
    GCATCTCAGT CCGCAGGAAG AAAAGCCAAG GTGAAGGCAG CCAGCAGCCT GGCCAACCTG
    GCAGAGGGAA GTAAAGACAG AAGCCCCATG TTCCCTGCTC TCGGTGAGAC AGGCAGTGAT
    GATGATCTCT TTCAGTCCAT TAAACCAAAA CCAGCAAAGA AAACAAATCC TTTCCCTTTC
    CTGGAAGATG AGGACGATCT GTTTACAGAC CAGAGAGGCA AGAAGAATGG GTCAAAATCC
    AACAGTCAAC AGGATATTAT CTCAAAAGCA CAAGATATAT TTGAGGATGA TATATTTGCT
    ACAGAAGCAA ATAAACCCTC ACAAAAAACA AGAGAGAAGG AAAGAACATT TGACTCCAAC
    TTGTTCGATG ACAACATTGA TATCTTTGCT GACCTAACTG TAAAACCAAA AGAAAAGTCC
    AAAAAGAAAG TGGAAGCTAA GTCTATATTT GACGATGATA CAGATGATAT CTTCTCCTCT
    GGTATCCAGA CTAAGACAAC CAAATCAAAA AGTCGATCTT CACAGAAAGT GACTGAATCG
    AGATCTGGAC AGAAGGTTTC CAACATATTT GATGATCCCT TGAATGCCTT TGGAGGCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316553.1
    CDS108..3590
    Translation

    Target ORF information:

    RefSeq Version XM_015461067.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461067.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGTCAGGAA GCCCACCCAC
    AGTGGCTCGC AGCTGGTCTG TCCTGTCCTT CCACCCACAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGCAA ATTTAGCGAT CAACCCAGCA GCCTTGCTGC CTCCAGCAGC TCCCCAGATC
    TCTGGAATGA AGTCTGTTTT GCCAGAATCT GGTGCTCCTT CGTCGGAACC TGGGAGGATG
    CAGAGTCTGG ACCGTGTGCC CACTCCTCCT GGGAGTGGGG AGGCTGGTGT GAGCTTTGAC
    CTTCCAGCTC AGGCAGATAC CTTACACTGT GCGAACAAGA CCCGGGTCAA AGTGAGAGGG
    AAGCGCAGAC CGCAGACGCG CGCAGCTCGA CACCTGGCTG CCCAGGAGTC CACTGAGGCC
    GAGGATGTGG GTTCCCCCAG GGGATTTGTG GCACAGTTGA CAGATGGTGC CACATCACCA
    GATGGTCGTC AGCCACCGCT GAGGGCAGCT GGTGGAGCAG AAGAAGCAGT GGCAGCTGCC
    ACTCCAACAT GGGCAGGTGG TCCTGTGCCT GGAATGAACA GAGGCCCATT TGTGAAATCT
    GTGGGTCAAC CTCTTGAGGA TGATCTGTTT GATTCTGGAG ATATCTTTTC CAAGGGCATT
    GCATCTCAGT CCGCAGGAAG AAAAGCCAAG GTGAAGGCAG CCAGCAGCCT GGCCAACCTG
    GCAGAGGGAA GTAAAGACAG AAGCCCCATG TTCCCTGCTC TCGGTGAGAC AGGCAGTGAT
    GATGATCTCT TTCAGTCCAT TAAACCAAAA CCAGCAAAGA AAACAAATCC TTTCCCTTTC
    CTGGAAGATG AGGACGATCT GTTTACAGAC CAGAGAGGCA AGAAGAATGG GTCAAAATCC
    AACAGTCAAC AGGATATTAT CTCAAAAGCA CAAGATATAT TTGAGGATGA TATATTTGCT
    ACAGAAGCAA ATAAACCCTC ACAAAAAACA AGAGAGAAGG AAAGAACATT TGACTCCAAC
    TTGTTCGATG ACAACATTGA TATCTTTGCT GACCTAACTG TAAAACCAAA AGAAAAGTCC
    AAAAAGAAAG TGGAAGCTAA GTCTATATTT GACGATGATA CAGATGATAT CTTCTCCTCT
    GGTATCCAGA CTAAGACAAC CAAATCAAAA AGTCGATCTT CACAGAAAGT GACTGAATCG
    AGATCTGGAC AGAAGGTTTC CAACATATTT GATGATCCCT TGAATGCCTT TGGAGGCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34349
    Clone ID Related Accession (Same CDS sequence) XM_005226575.3
    Accession Version XM_005226575.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226632.1
    CDS108..4046
    Translation

    Target ORF information:

    RefSeq Version XM_005226575.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226575.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGCAAATTT AGCGATCAAC
    CCAGCAGCCT TGCTGCCTCC AGCAGCTCCC CAGATCTCTG GAATGAAGTC TGTTTTGCCA
    GAATCTGGTG CTCCTTCGTC GGAACCTGGG AGGATGCAGA GTCTGGACCG TGTGCCCACT
    CCTCCTGGGA GTGGGGAGGC TGGTGTGAGC TTTGACCTTC CAGCTCAGGC AGATACCTTA
    CACTGTGCGA ACAAGACCCG GGTCAAAGTG AGAGGGAAGC GCAGACCGCA GACGCGCGCA
    GCTCGACACC TGGCTGCCCA GGAGTCCACT GAGGCCGAGG ATGTGGGTTC CCCCAGGGGA
    TTTGTGGCAC AGTTGACAGA TGGTGCCACA TCACCAGATG GTCGTCAGCC ACCGCTGAGG
    GCAGCTGGTG GAGCAGAAGA AGCAGTGGCA GCTGCCACTC CAACATGGGC AGGTGGTCCT
    GTGCCTGGAA TGAACAGAGG CCCATTTGTG AAATCTGTGG GTCAACCTCT TGAGGATGAT
    CTGTTTGATT CTGGAGATAT CTTTTCCAAG GGCATTGCAT CTCAGTCCGC AGGAAGAAAA
    GCCAAGGTGA AGGCAGCCAG CAGCCTGGCC AACCTGGCAG AGGGAAGTAA AGACAGAAGC
    CCCATGTTCC CTGCTCTCGG TGAGACAGGC AGTGATGATG ATCTCTTTCA GTCCATTAAA
    CCAAAACCAG CAAAGAAAAC AAATCCTTTC CCTTTCCTGG AAGATGAGGA CGATCTGTTT
    ACAGACCAGA GAGGCAAGAA GAATGGGTCA AAATCCAACA GTCAACAGGA TATTATCTCA
    AAAGCACAAG ATATATTTGA GGATGATATA TTTGCTACAG AAGCAAATAA ACCCTCACAA
    AAAACAAGAG AGAAGGAAAG AACATTTGAC TCCAACTTGT TCGATGACAA CATTGATATC
    TTTGCTGACC TAACTGTAAA ACCAAAAGAA AAGTCCAAAA AGAAAGTGGA AGCTAAGTCT
    ATATTTGACG ATGATACAGA TGATATCTTC TCCTCTGGTA TCCAGACTAA GACAACCAAA
    TCAAAAAGTC GATCTTCACA GAAAGTGACT GAATCGAGAT CTGGACAGAA GGTTTCCAAC
    ATATTTGATG ATCCCTTGAA TGCCTTTGGA GGCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38695
    Clone ID Related Accession (Same CDS sequence) NM_001205767.1
    Accession Version NM_001205767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02062236.1 and DAAA02062238.1. On Apr 20, 2011 this sequence version replaced XM_591483.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403652 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192696.1
    CDS103..4104
    Translation

    Target ORF information:

    RefSeq Version NM_001205767.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGAGTGACCT CTTCGCAGAA
    GCCCCTCAGG ATCGGGGAGC TCGAACCCCT GTTAATGAAG CATCTTCATC TTCTAAACCT
    GGAAAGAAAA TCCCGGCAGG AGCTGTTTCT GTATTTTTAG GGGATACTGA TGTGTTTGGT
    GCCACCTCTG CCCCGGCACC GAGGGAGCCC CAGAAGCACG AGCGGCCCCC TCCAGGGAAA
    AGCGCGTACG TCCCAGCGTC GGCTGGCCTC TTCGATGATG ACGACGACGA TGATGACTTT
    TTCTCGGCAT CCCGTAGCAA ACCTCCCAAG ACAGACAAAG TCGAGTCCAC TGCCGGTATC
    TTTGACGATG AAGAAGGAGA TCTGTTCAAA GAAAGAACAG TGGCTTTGCC AGAAGCTACT
    GTGAATCAGA CAGATGGAAA CAAAGCAAGA GCAGAAACAA AGGTTATGCT ACCTTCCAGC
    AAAAATCTCA AGCTCTCGTC GGAAACAAAG ACTCACAAAG GTTTATTTTC AGATGAGGAG
    GACTCTGAGG ATTTGTTTTC TTCTCAAAGT GTGAGTAAGT CAAAAGATGC AAGTGTCCTG
    CCTAGCAAAC ACCGCAGCTC AGTCTTGCTT TTTGATGATG AAGATGGACA GGATAATCTT
    TTTGATACTC CAGCTGCTAA GAAGCAGACA TCGTCTCTAC CGACTCAGAG CCAAAAGAAA
    ACAAAGCCCT CTGAGCCTCC CAAAAAGAAA GCATCTGCCT TACTGTTCAG CAGTGATGAG
    GAGGACCAGT GGCATGTTAC TGACTCACAG ACCAACTCCA CATCTGACAA GTCTAAAGGA
    GAACCTAGGG ACCCTGAGCC CACCCACCAG GAGGTTAAGG CCGTGAAAAA GACCAGCCTG
    TTTGAGGAGG ATGACGAGGA TGACCTGTTT GCTATTGCCA AGGACAGCCA AAAGAAGGCC
    CAGAGAGCAT CACTTCTGTT TGAAGATGAT GCTGACAGCG GAAGCTCTCT GTTCGGCTCT
    CCTCCCACAT CTGTTCCTCC TGCAACAACG AAAAAAGAGA CTGTCCCTGA AGTACCGCCT
    TTGCTGTTCA GCGATGAGGA AGAAAAGGCG GCTCAGTCTG GAGTGAAACC TGCAGCTAAG
    AAGGCTGGGA GCGCCAAGGG GACCTCAGAA CTTGGGAGAA CTCCTGCGGT CGAGGAGTCA
    GGAAAGGAAG GATCTTTGAA TGTACCTACT CAGGAAACAG CCAAGAACTC TGATTTATTT
    TCTTCATCAT CCCCATTGGA CAAAGGAACT AAAAGTAGAA CCAAAACGGT TCTTAGCTTA
    TTTGATGAGG AAGAGGATAA AACGGAAGAT CAAAGCAGCT TCCAAGCTCT GAGAAAAGAA
    GTAGGAAAGA GTCCTGATCC TGGTACCCGC CCCAAGAGCA CAGGTGTCTT TCAGGATGAA
    GAGCTCCTTT TTAGTCACAA GCTCCAAAAG GACAACGACC CAGATGTTGA CCTTTTCTCT
    GGCACCAAAA AATCCAGGTT GTTAGAGCCC AGTGTTGGGA GCCTGTTTGG AGATGATGAA
    GACGATGATC TTTTCAGCTC TGCCAAGTCG CAGCCTTTGA TCCCAGAAAA GAAGAAGGTA
    GTGAAAAAAG ACAACTCTAT TTCCTCTGTC AAGAACCAGA AACATCCTGA GTCCACTCAG
    GGTATCAAAG AAAAAGACGT ATGGAAATCG GAAACACCTC AGGACTCACC AGGTCCCGCT
    CCACTTAAAA CCAAAGAGCC ATCCCCTCGG ATCTGGAAAA TACAAGCAAA TTTAGCGATC
    AACCCAGCAG CCTTGCTGCC TCCAGCAGCT CCCCAGATCT CTGGAATGAA GTCTGTTTTG
    CCAGAATCTG GTGCTCCTTC GTCGGAACCT GGGAGGATGC AGAGTCTGGA CCGTGTGCCC
    ACTCCTCCTG GGAGTGGGGA GGCTGGTGTG AGCTTTGACC TTCCAGCTCA GGCAGATACC
    TTACACTGTG CGAACAAGAC CCGGGTCAAA GTGAGAGGGA AGCGCAGACC GCAGACGCGC
    GCAGCTCGAC ACCTGGCTGC CCAGGAGTCC ACTGAGGCCG AGGATGTGGG TTCCCCCAGG
    GGATTTGTGG CACAGTTGAC AGATGGTGCC ACATCACCAG ATGGTCGTCA GCCACCGCTG
    AGGGCAGCTG GTGGAGCAGA AGAAGCAGTG GCAGCTGCCA CTCCAACATG GGCAGGTGGT
    CCTGTGCCTG GAATGAACAG AGGCCCATTT GTGAAATCTG TGGGTCAACC TCTTGAGGAT
    GATCTGTTTG ATTCTGGAGA TATCTTTTCC AAGGGCATTG CATCTCAGTC CGCAGGAAGA
    AAAGCCAAGG TGAAGGCAGC CAGCAGCCTG GCCAACCTGG CAGAGGGAAG TAAAGACAGA
    AGCCCCATGT TCCCTGCTCT CGGTGAGACA GGCAGTGATG ATGATCTCTT TCAGTCCATT
    AAACCAAAAC CAGCAAAGAA AACAAATCCT TTCCCTTTCC TGGAAGATGA GGACGATCTG
    TTTACAGACC AGAGAGGCAA GAAGAATGGG TCAAAATCCA ACAGTCAACA GGATATTATC
    TCAAAAGCAC AAGATATATT TGAGGATGAT ATATTTGCTA CAGAAGCAAA TAAACCCTCA
    CAAAAAACAA GAGAGAAGGA AAGAACATTT GACTCCAACT TGTTCGATGA CAACATTGAT
    ATCTTTGCTG ACCTAACTGT AAAACCAAAA GAAAAGTCCA AAAAGAAAGT GGAAGCTAAG
    TCTATATTTG ACGATGATAC AGATGATATC TTCTCCTCTG GTATCCAGAC TAAGACAACC
    AAATCAAAAA GTCGATCTTC ACAGAAAGTG ACTGAATCGA GATCTGGACA GAAGGTTTCC
    AACATATTTG ATGATCCCTT GAATGCCTTT GGAGGCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34346
    Clone ID Related Accession (Same CDS sequence) XM_005226572.3
    Accession Version XM_005226572.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WASH complex subunit FAM21 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGGGGATAC TGATGTGTTT
    GGTGCCACCT CTGCCCCGGC ACCGAGGGAG CCCCAGAAGC ACGAGCGGCC CCCTCCAGGG
    AAAAGCGCGT ACGTCCCAGC GTCGGCTGGC CTCTTCGATG ATGACGACGA CGATGATGAC
    TTTTTCTCGG CATCCCGTAG CAAACCTCCC AAGACAGACA AAGTCGAGTC CACTGCCGGT
    ATCTTTGACG ATGAAGAAGG AGATCTGTTC AAAGAAAGAA CAGTGGCTTT GCCAGAAGCT
    ACTGTGAATC AGACAGATGG AAACAAAGCA AGAGCAGAAA CAAAGGTTAT GCTACCTTCC
    AGCAAAAATC TCAAGCTCTC GTCGGAAACA AAGACTCACA AAGGTTTATT TTCAGATGAG
    GAGGACTCTG AGGATTTGTT TTCTTCTCAA AGTGTGAGTA AGTCAAAAGA TGCAAGTGTC
    CTGCCTAGCA AACACCGCAG CTCAGTCTTG CTTTTTGATG ATGAAGATGG ACAGGATAAT
    CTTTTTGATA CTCCAGCTGC TAAGAAGCAG ACATCGTCTC TACCGACTCA GAGCCAAAAG
    AAAACAAAGC CCTCTGAGCC TCCCAAAAAG AAAGCATCTG CCTTACTGTT CAGCAGTGAT
    GAGGAGGACC AGTGGCATGT TACTGACTCA CAGACCAACT CCACATCTGA CAAGTCTAAA
    GGAGAACCTA GGGACCCTGA GCCCACCCAC CAGGAGGTTA AGGCCGTGAA AAAGACCAGC
    CTGTTTGAGG AGGATGACGA GGATGACCTG TTTGCTATTG CCAAGGACAG CCAAAAGAAG
    GCCCAGAGAG CATCACTTCT GTTTGAAGAT GATGCTGACA GCGGAAGCTC TCTGTTCGGC
    TCTCCTCCCA CATCTGTTCC TCCTGCAACA ACGAAAAAAG AGACTGTCCC TGAAGTACCG
    CCTTTGCTGT TCAGCGATGA GGAAGAAAAG GCGGCTCAGT CTGGAGTGAA ACCTGCAGCT
    AAGAAGGCTG GGAGCGCCAA GGGGACCTCA GAACTTGGGA GAACTCCTGC GGTCGAGGAG
    TCAGGAAAGG AAGGATCTTT GAATGTACCT ACTCAGGAAA CAGCCAAGAA CTCTGATTTA
    TTTTCTTCAT CATCCCCATT GGACAAAGGA ACTAAAAGTA GAACCAAAAC GGTTCTTAGC
    TTATTTGATG AGGAAGAGGA TAAAACGGAA GATCAAAGCA GCTTCCAAGC TCTGAGAAAA
    GAAGTAGGAA AGAGTCCTGA TCCTGGTACC CGCCCCAAGA GCACAGGTGT CTTTCAGGAT
    GAAGAGCTCC TTTTTAGTCA CAAGCTCCAA AAGGACAACG ACCCAGATGT TGACCTTTTC
    TCTGGCACCA AAAAATCCAG GTTGTTAGAG CCCAGTGTTG GGAGCCTGTT TGGAGATGAT
    GAAGACGATG ATCTTTTCAG CTCTGCCAAG TCGCAGCCTT TGATCCCAGA AAAGAAGAAG
    GTAGTGAAAA AAGACAACTC TATTTCCTCT GTCAAGAACC AGAAACATCC TGAGTCCACT
    CAGGGTATCA AAGAAAAAGA CGTATGGAAA TCGGAAACAC CTCAGGACTC ACCAGGTCCC
    GCTCCACTTA AAACCAAAGA GCCATCCCCT CGGATCTGGA AAATACAAGC AAATTTAGCG
    ATCAACCCAG CAGCCTTGCT GCCTCCAGCA GCTCCCCAGA TCTCTGGAAT GAAGTCTGTT
    TTGCCAGAAT CTGGTGCTCC TTCGTCGGAA CCTGGGAGGA TGCAGAGTCT GGACCGTGTG
    CCCACTCCTC CTGGGAGTGG GGAGGCTGGT GTGAGCTTTG ACCTTCCAGC TCAGGCAGAT
    ACCTTACACT GTGCGAACAA GACCCGGGTC AAAGTGAGAG GGAAGCGCAG ACCGCAGACG
    CGCGCAGCTC GACACCTGGC TGCCCAGGAG TCCACTGAGG CCGAGGATGT GGGTTCCCCC
    AGGGGATTTG TGGCACAGTT GACAGATGGT GCCACATCAC CAGATGGTCG TCAGCCACCG
    CTGAGGGCAG CTGGTGGAGC AGAAGAAGCA GTGGCAGCTG CCACTCCAAC ATGGGCAGGT
    GGTCCTGTGC CTGGAATGAA CAGAGGCCCA TTTGTGAAAT CTGTGGGTCA ACCTCTTGAG
    GATGATCTGT TTGATTCTGG AGATATCTTT TCCAAGGGCA TTGCATCTCA GTCCGCAGGA
    AGAAAAGCCA AGGTGAAGGC AGCCAGCAGC CTGGCCAACC TGGCAGAGGG AAGTAAAGAC
    AGAAGCCCCA TGTTCCCTGC TCTCGGTGAG ACAGGCAGTG ATGATGATCT CTTTCAGTCC
    ATTAAACCAA AACCAGCAAA GAAAACAAAT CCTTTCCCTT TCCTGGAAGA TGAGGACGAT
    CTGTTTACAG ACCAGAGAGG CAAGAAGAAT GGGTCAAAAT CCAACAGTCA ACAGGATATT
    ATCTCAAAAG CACAAGATAT ATTTGAGGAT GATATATTTG CTACAGAAGC AAATAAACCC
    TCACAAAAAA CAAGAGAGAA GGAAAGAACA TTTGACTCCA ACTTGTTCGA TGACAACATT
    GATATCTTTG CTGACCTAAC TGTAAAACCA AAAGAAAAGT CCAAAAAGAA AGTGGAAGCT
    AAGTCTATAT TTGACGATGA TACAGATGAT ATCTTCTCCT CTGGTATCCA GACTAAGACA
    ACCAAATCAA AAAGTCGATC TTCACAGAAA GTGACTGAAT CGAGATCTGG ACAGAAGGTT
    TCCAACATAT TTGATGATCC CTTGAATGCC TTTGGAGGCC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226629.1
    CDS108..4112
    Translation

    Target ORF information:

    RefSeq Version XM_005226572.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 21, member A (FAM21A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226572.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAACC GGACCACCCC CGACCAGGAG CGAGCGCCGG CGTCCGAGCC CGTGTGGGAG 
    CGTCCCTGGT CGGTGGAGGA GATCCGCAGG AGCAGCCAGA GCTGGTCGCT GGCGGCCGAC
    GCGGGCCTAC TACAGTTTCT ACAGGAATTT TCACAACAGA CTATCTCTAG GACTCATGAA
    ATCAAGAAAC AGGTGGATGG ACTAATTCGG GAGACTAAAG CCACAGATTG CCGCCTGCAT
    AATGTCTTCA ATGAGTTCCT TATGCTGTCT AATACCCAGT TCATTGAAAA CCGTGTATAT
    GACGAGGAGG TGGAGGAGCC GGTGCTCAAG ACTGAGGCGG AAAAACCAGA ACAGGAAAAG
    ACCCGGGAAC AGAAAGAAGT AGATCTGATT CCTAAAGTCC AGGAGGCCGT GAACTACGGT
    TTACAGGTGT TGGACAGTGC GTTTGAGCAA CTAGATATAA AAGCGGGAAA CTCAGACTCT
    GAAGAAGATG ATGCTAATGA GCGGCTGGAA TTGATCCTTG AGCCAAAGGA TCTGTACATT
    GACCGCCCTT TACCTTATTT AATTGGGTCA AAGCTGTTCA TGGAGCAGGA AGATGTTGGT
    CTTGGAGAGC TTTCTAGTGA AGAAGGTTCA GTAGGCAGTG ATCGTGGGAG TATCGCAGAC
    AGTGAAGAAG AGAACGAGGA AGAGGAGTCA GATGAAGATT TTGCCAATCA TAGTGACAAT
    GATCAAAACC GGCACATTGC AACGATGAGT GATGAAGAAG AGGATGATGA TGGTTGCGAC
    ATTTTTGCTG ACTCTGAGAA GGAGGAGGAA GATGTTGAAG ACATGGAAGA AAACTCTAGG
    CCTAAAAGAA GCAGGCCTAC ATCATTTGCT GATGAACTGG CAGCTCGGAT CAAAGGGGAC
    GCCTCGAGCA GGATGGAAGG GGAGCGCACA GCCTTATCCC CAGGGGAAGC AAAACCTCAG
    AAGACGTTGA AAGAGAAGAA GGAAAGGAAA ACTCCCTCAG ATGATGAAGA AGATAACTTA
    TTCGCACCCC CGAAGCTGAC AGATGAAGAC TTCTCGCCGT TTGGTTCTAG AGGCGGCCTG
    TTCAGTGGAG GGAAGGGACT TTTTGATGAT GAGGATGAGG AGCAGAGTGA CCTCTTCGCA
    GAAGCCCCTC AGGATCGGGG AGCTCGAACC CCTGTTAATG AAGCATCTTC ATCTTCTAAA
    CCTGGAAAGA AAATCCCGGC AGGAGCTGTT TCTGTATTTT TAGGGGATAC TGATGTGTTT
    GGTGCCACCT CTGCCCCGGC ACCGAGGGAG CCCCAGAAGC ACGAGCGGCC CCCTCCAGGG
    AAAAGCGCGT ACGTCCCAGC GTCGGCTGGC CTCTTCGATG ATGACGACGA CGATGATGAC
    TTTTTCTCGG CATCCCGTAG CAAACCTCCC AAGACAGACA AAGTCGAGTC CACTGCCGGT
    ATCTTTGACG ATGAAGAAGG AGATCTGTTC AAAGAAAGAA CAGTGGCTTT GCCAGAAGCT
    ACTGTGAATC AGACAGATGG AAACAAAGCA AGAGCAGAAA CAAAGGTTAT GCTACCTTCC
    AGCAAAAATC TCAAGCTCTC GTCGGAAACA AAGACTCACA AAGGTTTATT TTCAGATGAG
    GAGGACTCTG AGGATTTGTT TTCTTCTCAA AGTGTGAGTA AGTCAAAAGA TGCAAGTGTC
    CTGCCTAGCA AACACCGCAG CTCAGTCTTG CTTTTTGATG ATGAAGATGG ACAGGATAAT
    CTTTTTGATA CTCCAGCTGC TAAGAAGCAG ACATCGTCTC TACCGACTCA GAGCCAAAAG
    AAAACAAAGC CCTCTGAGCC TCCCAAAAAG AAAGCATCTG CCTTACTGTT CAGCAGTGAT
    GAGGAGGACC AGTGGCATGT TACTGACTCA CAGACCAACT CCACATCTGA CAAGTCTAAA
    GGAGAACCTA GGGACCCTGA GCCCACCCAC CAGGAGGTTA AGGCCGTGAA AAAGACCAGC
    CTGTTTGAGG AGGATGACGA GGATGACCTG TTTGCTATTG CCAAGGACAG CCAAAAGAAG
    GCCCAGAGAG CATCACTTCT GTTTGAAGAT GATGCTGACA GCGGAAGCTC TCTGTTCGGC
    TCTCCTCCCA CATCTGTTCC TCCTGCAACA ACGAAAAAAG AGACTGTCCC TGAAGTACCG
    CCTTTGCTGT TCAGCGATGA GGAAGAAAAG GCGGCTCAGT CTGGAGTGAA ACCTGCAGCT
    AAGAAGGCTG GGAGCGCCAA GGGGACCTCA GAACTTGGGA GAACTCCTGC GGTCGAGGAG
    TCAGGAAAGG AAGGATCTTT GAATGTACCT ACTCAGGAAA CAGCCAAGAA CTCTGATTTA
    TTTTCTTCAT CATCCCCATT GGACAAAGGA ACTAAAAGTA GAACCAAAAC GGTTCTTAGC
    TTATTTGATG AGGAAGAGGA TAAAACGGAA GATCAAAGCA GCTTCCAAGC TCTGAGAAAA
    GAAGTAGGAA AGAGTCCTGA TCCTGGTACC CGCCCCAAGA GCACAGGTGT CTTTCAGGAT
    GAAGAGCTCC TTTTTAGTCA CAAGCTCCAA AAGGACAACG ACCCAGATGT TGACCTTTTC
    TCTGGCACCA AAAAATCCAG GTTGTTAGAG CCCAGTGTTG GGAGCCTGTT TGGAGATGAT
    GAAGACGATG ATCTTTTCAG CTCTGCCAAG TCGCAGCCTT TGATCCCAGA AAAGAAGAAG
    GTAGTGAAAA AAGACAACTC TATTTCCTCT GTCAAGAACC AGAAACATCC TGAGTCCACT
    CAGGGTATCA AAGAAAAAGA CGTATGGAAA TCGGAAACAC CTCAGGACTC ACCAGGTCCC
    GCTCCACTTA AAACCAAAGA GCCATCCCCT CGGATCTGGA AAATACAAGC AAATTTAGCG
    ATCAACCCAG CAGCCTTGCT GCCTCCAGCA GCTCCCCAGA TCTCTGGAAT GAAGTCTGTT
    TTGCCAGAAT CTGGTGCTCC TTCGTCGGAA CCTGGGAGGA TGCAGAGTCT GGACCGTGTG
    CCCACTCCTC CTGGGAGTGG GGAGGCTGGT GTGAGCTTTG ACCTTCCAGC TCAGGCAGAT
    ACCTTACACT GTGCGAACAA GACCCGGGTC AAAGTGAGAG GGAAGCGCAG ACCGCAGACG
    CGCGCAGCTC GACACCTGGC TGCCCAGGAG TCCACTGAGG CCGAGGATGT GGGTTCCCCC
    AGGGGATTTG TGGCACAGTT GACAGATGGT GCCACATCAC CAGATGGTCG TCAGCCACCG
    CTGAGGGCAG CTGGTGGAGC AGAAGAAGCA GTGGCAGCTG CCACTCCAAC ATGGGCAGGT
    GGTCCTGTGC CTGGAATGAA CAGAGGCCCA TTTGTGAAAT CTGTGGGTCA ACCTCTTGAG
    GATGATCTGT TTGATTCTGG AGATATCTTT TCCAAGGGCA TTGCATCTCA GTCCGCAGGA
    AGAAAAGCCA AGGTGAAGGC AGCCAGCAGC CTGGCCAACC TGGCAGAGGG AAGTAAAGAC
    AGAAGCCCCA TGTTCCCTGC TCTCGGTGAG ACAGGCAGTG ATGATGATCT CTTTCAGTCC
    ATTAAACCAA AACCAGCAAA GAAAACAAAT CCTTTCCCTT TCCTGGAAGA TGAGGACGAT
    CTGTTTACAG ACCAGAGAGG CAAGAAGAAT GGGTCAAAAT CCAACAGTCA ACAGGATATT
    ATCTCAAAAG CACAAGATAT ATTTGAGGAT GATATATTTG CTACAGAAGC AAATAAACCC
    TCACAAAAAA CAAGAGAGAA GGAAAGAACA TTTGACTCCA ACTTGTTCGA TGACAACATT
    GATATCTTTG CTGACCTAAC TGTAAAACCA AAAGAAAAGT CCAAAAAGAA AGTGGAAGCT
    AAGTCTATAT TTGACGATGA TACAGATGAT ATCTTCTCCT CTGGTATCCA GACTAAGACA
    ACCAAATCAA AAAGTCGATC TTCACAGAAA GTGACTGAAT CGAGATCTGG ACAGAAGGTT
    TCCAACATAT TTGATGATCC CTTGAATGCC TTTGGAGGCC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.