NOTCH2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOTCH2
Entrez Gene ID	513730
Full Name	notch 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015462612.1	XP_015318098.1	neurogenic locus notch homolog protein 2 isoform X2
XM_002686114.5	XP_002686160.2	neurogenic locus notch homolog protein 2 isoform X1
XM_024990004.1	XP_024845772.1	neurogenic locus notch homolog protein 2 isoform X4
XM_015463442.2	XP_015318928.2	neurogenic locus notch homolog protein 2 isoform X2
XM_867242.8	XP_872335.5	neurogenic locus notch homolog protein 2 isoform X1
XM_024990003.1	XP_024845771.1	neurogenic locus notch homolog protein 2 isoform X3
XM_015463442.1	XP_015318928.1	neurogenic locus notch homolog protein 2 isoform X2
XM_002686114.6	XP_002686160.3	neurogenic locus notch homolog protein 2 isoform X1

Pathways from BioSystems

Homologies

	NOTCH2	XP_001114248.1
Rattus norvegicus (Norway rat)	Notch2	NP_077334.1
Homo sapiens (human)	NOTCH2	NP_077719.2
Canis lupus familiaris (dog)	NOTCH2	XP_005630745.1
Bos taurus (cattle)	NOTCH2	XP_002686160.2
Xenopus tropicalis (tropical clawed frog)	LOC100486344	XP_002939126.2
Pan troglodytes (chimpanzee)	NOTCH2	XP_513697.3
Mus musculus (house mouse)	Notch2	NP_035058.2
Gallus gallus (chicken)	NOTCH2	NP_001238962.1
Danio rerio (zebrafish)	notch2	NP_001108566.1

Gene Ontology

The following NOTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa03359	XM_002686114.6 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa233210	XM_015463442.2 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233211	XM_024990003.1 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233212	XM_024990004.1 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa89538	XM_015463442.1 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa89538	XM_015462612.1 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa17547	XM_867242.8 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa17547	XM_002686114.5 Latest version!	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOTCH2 ( XM_002686114.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686160.3 Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa03359
Clone ID Related Accession (Same CDS sequence)	XM_002686114.6
Accession Version	XM_002686114.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002686114.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACGCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686160.3
CDS	262..7677
Translation	MPALRPAPLWALLALWLCRAAPARALQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKRTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_002686114.6
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686114.6

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACGCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_015463442.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318928.2 Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

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CloneID	OBa233210
Clone ID Related Accession (Same CDS sequence)	XM_015463442.2
Accession Version	XM_015463442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015463442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACGCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTGCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318928.2
CDS	16..7404
Translation	MANSPQPFTTSIYSATLQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKRTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_015463442.2
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463442.2

ORF Insert Sequence:

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACGCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTGCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_024990003.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845771.1 Bos taurus notch 2 (NOTCH2), transcript variant X3, mRNA.

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CloneID	OBa233211
Clone ID Related Accession (Same CDS sequence)	XM_024990003.1
Accession Version	XM_024990003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCT GCCCAGGCGG CGGCGGCGGA GGAGGAGGCG AACGAGAAGA TGCCCGCCCT 
GCGCCCCGCT CCGCTGTGGG CGCTGCTGGC ACTGTGGCTG TGCCGCGCGG CCCCGGCGCG 
TGGTGTCCAG AGGGCTTCTT GGGAGAATAT TGTCAGCATC GAGACCCCTG TGAGAAGAAC 
CGTTGCCAGA ATGGTGGGAC GTGTGTGGCC CAGGCTATGT TGGGGAAAGC CACGTGCCGC 
TGTGCCCCGG GCTTCACGGG CGAGGACTGC CAGCACTCGA CTACTCACCC CTGCTTTGTG 
TCTCACCCCT GCCTGAATGG GGGCACCTGC CACGTGCTTA GCCGGGACAC CTACGAGTGT 
ACCTGCCAAG TCGGTTTTAC AGGTAAACTG TGCCAGTGGA CTGATGCCTG CCTGTCCCAC 
CCCTGTGCAA ACGGAAGTAC CTGTACCACT ATGGCCAACC AGTTCTCCTG CACATGCCTT 
GCAGGCTTCA CAGGGCAGAA ATGTGAGACT GATGTCAATG AGTGTGACAT TCCAGGACAG 
TGCCAGCACG GTGGCACCTG CCTCAACCTC CCAGGCTCCT ATCAGTGCCA ATGCCCCCAG 
GGCTTCACAG GCCAGCACTG CGACAGTCCC TATGTGCCCT GCGCACCCTC CCCCTGTGTC 
AACGGAGGTA CCTGCCGGCA GACCGGTGAC TTTACCTTTG AGTGCAGTTG CCTTCCAGGT 
TTTGAAGGAG TCACCTGTGA GCGGAATATA GATGACTGTC CTAACCACAA GTGTCAGAAT 
GGAGGGGTTT GTGTGGATGG GGTCAATACC TACAACTGCC GCTGTCCCCC TCAGTGGACA 
GGACAGTTCT GCACAGAGGA TGTGGATGAG TGCCTGCTGC AGCCCAATGC CTGTCAGAAC 
GGGGGCACCT GTACCAACCG CAACGGGGGC TATGGCTGTG TATGTGTGAA TGGCTGGAGT 
GGGGACGACT GCAGTGAGAA TATCGATGAC TGTGCCTTCG CCTCCTGCAC CCCGGGCTCT 
ACCTGCATCG ATCGTGTGGC CTCCTTCTCT TGTATGTGCC CAGAAGGAAA GGCAGGTCTC 
TTATGTCATC TGGATGACGC GTGCATCAGC AACCCCTGCC ACAAGGGGGC GCTGTGTGAT 
ACCAACCCTC TGAATGGACA GTATATTTGC ACCTGCCCAC AAGGCTACAA AGGGAGTGAC 
TGTACAGAAG ATGTGGATGA GTGTGCCATG GCCAACAGCA ATCCTTGTGA GCATGCAGGA 
AAATGTGTGA ACACAGATGG TGCCTTCCAC TGTGAGTGTC TGAAGGGCTA TGCAGGACCT 
CGTTGTGAGA TGGACATCAA TGAGTGCCAC TCAGACCCCT GCAAGAATGA TGCCACCTGT 
TTGGATAAGA TTGGAGGCTT CACGTGTCTG TGCATGCCAG GTTTCAAAGG TGTGCATTGT 
GAGTTGGAGA TAAATGAATG TCAGAGCAAC CCTTGTGTGA ACAATGGACA GTGTGTGGAT 
AAAGTCAATC GCTTCCAGTG TCTGTGTCCG CCTGGATTCA CGGGACCAGT TTGCCAGATT 
GATATTGATG ACTGTTCTAG TACTCCGTGT CTGAATGGGG CAAAGTGTAT AGATCACCCG 
AATGGCTATG AGTGCCAGTG TGCCACAGGA TTCACTGGTG TGTTGTGTGA GGAGAACATT 
GACAACTGTG ACCCTGATCC TTGCCACCAC GGCCAGTGTC AGGATGGCAT TGATTCCTAC 
ACCTGCATTT GCAATCCGGG CTACATGGGC GCCATCTGCA GTGACCAGAT AGACGAATGC 
TACAGCAGCC CTTGCCTGAA TGAGGGTCGC TGCATCGACC TGGTGAATGG TTACCAGTGC 
AACTGCCAGC CAGGCACGTC AGGTGTTAAT TGTGAAATCA ATTTTGATGA CTGTGCAAGT 
AACCCGTGTG TCCATGGAGT CTGTATGGAT GGCGTTAATC GTTACAGCTG TGTCTGCTCA 
CCAGGATTCA CAGGGCAGAG ATGTAACATT GACATTGATG AGTGTGCCTC CAATCCCTGT 
CGCAAGGGTG CAACGTGCAT CAATGATGTG AATGGTTTCC GCTGTATCTG CCCTGAGGGC 
CCCCACCATC CCAGCTGCTA CTCACAGGTG AACGAGTGCC TGAGCAATCC CTGTGTCCAT 
GGAAACTGTA CTGGGGGCCT CACTGGATAC AAGTGCCTCT GTGATGCAGG CTGGGTTGGC 
ATCAACTGTG AAGTGGACAA AAATGAATGT CTTTCTAATC CATGCCAGAA CGGGGGAACT 
TGTGACAATC TGGTGAATGG ATACAGGTGC ACTTGCAAGA AGGGCTTTAA AGGCTATAAC 
TGCCAGGTGA ATATTGATGA ATGTGCTTCA AATCCATGTC TGAACCAAGG AACCTGCCTT 
GATGACGTAA GCGGTTATAC TTGCCACTGT GTGCTGCCAT ACACAGGCAA GAACTGTCAG 
ACCGTATTGG CTCCCTGTTC CCCAAACCCT TGTGAGAATG CTGGTGTTTG CAAAGAGGCA 
CCAAATTTTG AGAGTTACTC CTGCCTGTGT GCTCCTGGCT GGCAAGGTCA GCGGTGTACC 
ATCGACATTG ATGAGTGTGT CTCCAAGCCC TGCATGAACC ACGGTCTCTG CCATAACACC 
CAGGGCAGCT ACATGTGTGA ATGTCCTCCA GGCTTCAGTG GCATGGACTG TGAGGAGGAC 
ATTGATGACT GCCTTGCCAA TCCTTGCCAG AATGGAGGTT CCTGTGTAGA TGGAGTGAAT 
ACTTTCTCCT GCCTGTGCCT TCCGGGCTTC ACTGGGGATA AATGTCAGAC AGACATGAAT 
GAGTGTCTGA GTGAGCCCTG TAAGAACGGA GGAACCTGCT CTGACTATGT CAACAGTTAC 
ACCTGCAAGT GCCCGGCGGG ATTTGATGGA GTCCACTGTG AGAACAACAT TGACGAGTGC 
ACTGAGAGCT CCTGTTTCAA TGGTGGCACG TGTATCGATG GGATTAACTC CTTCTCTTGC 
TTGTGCCCTG TGGGCTTCAC TGGTTCCTTC TGCCTCCATG AGATCAATGA ATGCAACTCT 
CATCCATGCC TGAATGAAGG AGTATGTGTT GATGGCCTGG GTACCTACCG CTGTATCTGC 
CCATTGGGCT ACACTGGAAA AAACTGTCAG ACCCTGGTGA ACCTCTGTAG TCGGTCTCCA 
TGTAAAAACA AAGGTACCTG CATTCAGGAT AAAGCAGAGT CGCGGTGCCT GTGTCCGTCT 
GGATGGGCTG GTGCTTACTG CGATGTTCCC AGTGTCTCCT GTGAGGTGGC GGCCTCCCAC 
AGAGGGGTGC CCGTTGACCG CTTGTGCCAG CACTCAGGCG TCTGTATCAG TGCGGGCAAC 
TCGCATCACT GCCAGTGCCC CCTGGGCTAC ACCGGGAGCT ACTGTGAGGA CCAGCTTGAT 
GAGTGCTCGT CCAATCCCTG CCAGCACGGC GCCACCTGCC GGGACTTCAT TGGTGGATAC 
AGATGTGAGT GTGTCCCTGG ATATCAGGGT GTCAACTGCG AGTATGAAGT GGATGAGTGC 
CAGAACCAGC CATGCCAGAA TGGGGGCACC TGTATCGACC TTGTGAACCA CTTCAAGTGC 
TCCTGCCCAC CAGGCACTCG GGGCCTGCTT TGTGAAGAGA ACGTTGACGA CTGTGCCAGG 
GGTCCCCACT GCCTTAATGG TGGCCAGTGT GTAGACAGGA TTGGGGGCTA CAGTTGTCGC 
TGCTTGCCAG GCTTTGCTGG AGAACGCTGT GAAGGGGACA TCAACGAGTG CCTGTCCAAC 
CCCTGCAGCT CTGAGGGCAG CCTGGACTGC ATACAGCTCA CCAACGACTA CCTGTGCGTC 
TGCCGCAGCA CCTTCACTGG TCGTCACTGT GAAACGTTCG TCGACGTGTG TCCCCAGATG 
CCTTGCCTGA ACGGAGGCAC TTGTGCTGTG GCCAGCAACA TGCCCGATGG TTTCATCTGT 
CGTTGTCCCC CGGGCTTCTC TGGGGCACGA TGCCAGAGCA GCTGCGGACA GGTGAAGTGC 
AGGAGAGGGG AGCAATGTGT GCACACGGCC TCGGGCCCCC GCTGCTTCTG CCCCAACCCC 
CAGGACTGCG ACTCGGGCTG CGCCAGTAGC CCCTGCCAGC ACGGGGGGAG CTGCTACCCT 
CAGCGCCAGC CTCCTTACTA CTCGTGCCAG TGTGCCCCGC CGTTCCGGGG CAGCCACTGT 
GAACTCTACA CCGCCCCCAC CAGCACCCCT CCCGCCACCT GCCTGAGCCA GTATTGTGCT 
GACAAAGCTC GGGACGGCAT CTGTGATGAC GTGTGCAACA GCCATGCCTG CCAGTGGGAT 
GGGGGGGACT GTTCCCTCAC CATGGAGGAC CCCTGGGCCA ATTGCTCCTC CCCGCTGCCC 
TGCTGGGAGT ACATCAACAA CCAGTGCGAC GAGCTGTGCA ACACGGCCGA GTGCCTGTTT 
GACAACTTTG AATGCCAGAA GAGTAGCAAG ACATGCAAAT ATGACAAATA CTGTGCAGAC 
CACTACGAAG ACAACCATTG TGACCAGGGT TGCAACAGTG AGGAGTGTGG CTGGGATGGG 
CTGGACTGTG CTGCTGACCG GCCAGAAAAC CTGGCAGAGG GCACCCTGGT CATCGTGGTG 
CTGATGCCAC CTGAACAGCT GCTCCAGGAT GCCCGCAGAT TCTTGCGGGC ACTGGGCACC 
CTGCTCCACA CCAACCTCCG CATCAAACGG TCTGCCCAGG GGGAACTCAT GGTCTACCCC 
TATTATGGTG GGAAGTCAGC TGCCATGAAG AAGCAGAGAC TGACACGCAG GTCCCTTCCT 
GGAGATGAAG AACAGGAAGT GGCTGGCTCT CAGGTATTTC TGGAGATTGA CAACCGCCAG 
TGTGTTCAAG ACTCAGACCA GTGCTTTAAG AACACAGACG CAGCCGCAGC TCTTCTGGCT 
TCTCACGCCA TCCAGGGGAC CCTGTCGTAC CCACTTGTGT CTGTCGTCAG TGAATCTCTG 
ACCCCAAAAC GCACTCAGCT TCTCTATCTG CTTGCTGTTG CCGTTGTCAT TATCTTGTTT 
ATTATCCTGC TGGGGGTTAT CATGGCAAAA CGAAAGCGCA AGCATGGCTC CCTCTGGCTG 
CCTGAAGGTT TCACCCTTCG CCGTGACTCC AGCAACCACA AACGTCGTGA GCCAGTGGGC 
CAGGATGCTG TGGGACTCAA AAATCTTTCA GTGCAAGTCT CAGAGGCGAA CCTAATTGGT 
TCTGGAACAA GTGAACACTG GGTCGATGAT GAAGGGCCGC AGCCTAAGAA AGCCAAGGCT 
GAAGATGAGG CTTTGCTCTC AGAAGAAGAT GACCCCATCG ATCGGCGTCC ATGGACACAG 
CAGCACCTGG AAGCTGCAGA CATCCGTAGG ACACCATCGC TGGCTCTCAC CCCTCCACAG 
GCAGAGCAGG AGGTGGACGT GCTGGATGTG AACGTCCGCG GTCCAGATGG GTGCACCCCA 
CTGATGCTGG CTTCTCTCCG AGGAGGCAGC TCAGACATGA GTGATGAAGA TGAAGATGCA 
GAGGACTCTT CTGCCAACAT CATCACGGAC CTAGTCTACC AGGGTGCCAG CCTCCAAGCA 
CAGACAGACC GGACGGGTGA GATGGCCCTG CATCTCGCAG CCCGGTACTC AAGGGCAGAT 
GCTGCCAAGC GTCTCCTGGA TGCAGGTGCA GATGCCAATG CCCAGGACAA CATGGGCCGC 
TGCCCTCTCC ATGCTGCAGT GGCTGCTGAT GCCCAAGGTG TCTTCCAGAT ACTGATCCGC 
AACCGAGTAA CTGATCTAGA CGCAAGGATG AATGATGGCA CTACACCCCT GATCCTGGCT 
GCCCGCCTGG CAGTGGAGGG AATGGTGGCA GAACTGATCA ACTGCCAAGC AGATGTGAAT 
GCAGTGGATG ACCATGGAAA ATCTGCCCTT CACTGGGCAG CTGCTGTGAA TAATGTGGAG 
GCAACCCTTT TGCTGTTGAA AAATGGGGCC AACCGAGACA TGCAGGACAA CAAGGAGGAG 
ACACCCCTGT TTCTTGCTGC CCGGGAGGGG AGCTATGAAG CAGCCAAGAT CCTGTTAGAC 
CATTTTGCCA ATCGGGACAT CACAGACCAC ATGGATCGCC TTCCCCGGGA CGTGGCCCGG 
GACCGCATGC ACCATGACAT CGTGCGCCTG CTGGACGAGT ACAACGTGAC CCCCAGCCCT 
CCCGGCACAG TGCTGACTTC TGCGCTCTCA CCCGTCATCT GTGGGCCCAA CAGACCTTTT 
CTCAGTTTGA AGCATACCCC AATGGGCAAG AAGTCTAGAC GGCCTAACAC CAAGAGTACC 
GTGCCCACTA GCCTCCCTAA CCTTGCCAAG GAGGCCAAGG ATGCCAAGGG GAACAGGAGG 
AAGAAGTCTC TGAGTGACAA GGGCCAGCTG TCCGAGAGCT CAGTGACTCT GTCCCCCGTC 
GATTCCCTAG AATCTCCGCA CACATATGTT TCTGACACCA CCTCCTCTCC AATGATCACA 
TCCCCCGGAA TCTTACAGGC CTCCCCCAAC CCCATGCTGG CCACTGCCGC CCCCACTGCC 
CCAGTCCATG CACAGCATGC ACTGTCTTTC TCTAACCTTC ATGAGATGCA GCCCTTGGCT 
CATGGGGCCA ACACTGTGCT TCCCTCGGTG AGCCAGCTGC TGTCTCACCG CCACATTGTG 
CCCCCGGGCA GCAGCACCGC AGGGAGCTTG GGTAGGCTGC ATTCAGTCAC TGTCCCCGCC 
GACTGGATGA ACCGCGTGGA GATGAATGAG AGCCAGTACA ACGAGATGTT CGGTATGGTC 
CTGGCTCCAG CTGAGGGCAC CCACCCTGGC ATAGCTCCCC AGAGCAGGCC GCCCGAAGGG 
AAGCATTTAA CCACCCCACG AGAGCCCTTG CCCCCCATTG TGACTTTCCA GCTAATCCCC 
AAAGGCAGCA TTGCCCAACC AACTGGGGCT GCCCAGCCTC CGTCCAACTG CCCTCCAGCT 
GTTGCAGGCA CCCTGCCCAC CATGTATCAG ATTCCAGAAA TGGCTCGCTT GCCCAGTATG 
GCTTTTCCCA CTGCCATGAT GCCCCAGCAG GATGGGCAGG TTGCTCAGAC CATTCTCCCA 
GCCTATCACG CCTTCCCAGC CTCCGTGGGC AAGTATCCCA CACCCCCATC ACAGCATAGC 
TATGCTTCCT CCAACGCTGC TGAGAGAACG CCCAGTCACA GTGGTCACCT CCAGGGTGAG 
CATCCCTACC TGACACCGTC CCCAGAGTCT CCTGACCAGT GGTCAAGTTC ATCACCCCAC 
TCTGCTTCTG ACTGGTCAGA TGTGACCACC AGCCCCACTC CTGGGGGTGC TGGAGGAGGT 
CAGCGGGGAC CTGGGACACA CATGTCTGAG CCACCACGCA GCAACATGCA GGTCTATGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845771.1
CDS	213..7595
Translation	MWICPGGGGGGGGEREDARPAPRSAVGAAGTVAVPRGPGAWCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKRTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_024990003.1
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990003.1

ORF Insert Sequence:

ATGTGGATCT GCCCAGGCGG CGGCGGCGGA GGAGGAGGCG AACGAGAAGA TGCCCGCCCT 
GCGCCCCGCT CCGCTGTGGG CGCTGCTGGC ACTGTGGCTG TGCCGCGCGG CCCCGGCGCG 
TGGTGTCCAG AGGGCTTCTT GGGAGAATAT TGTCAGCATC GAGACCCCTG TGAGAAGAAC 
CGTTGCCAGA ATGGTGGGAC GTGTGTGGCC CAGGCTATGT TGGGGAAAGC CACGTGCCGC 
TGTGCCCCGG GCTTCACGGG CGAGGACTGC CAGCACTCGA CTACTCACCC CTGCTTTGTG 
TCTCACCCCT GCCTGAATGG GGGCACCTGC CACGTGCTTA GCCGGGACAC CTACGAGTGT 
ACCTGCCAAG TCGGTTTTAC AGGTAAACTG TGCCAGTGGA CTGATGCCTG CCTGTCCCAC 
CCCTGTGCAA ACGGAAGTAC CTGTACCACT ATGGCCAACC AGTTCTCCTG CACATGCCTT 
GCAGGCTTCA CAGGGCAGAA ATGTGAGACT GATGTCAATG AGTGTGACAT TCCAGGACAG 
TGCCAGCACG GTGGCACCTG CCTCAACCTC CCAGGCTCCT ATCAGTGCCA ATGCCCCCAG 
GGCTTCACAG GCCAGCACTG CGACAGTCCC TATGTGCCCT GCGCACCCTC CCCCTGTGTC 
AACGGAGGTA CCTGCCGGCA GACCGGTGAC TTTACCTTTG AGTGCAGTTG CCTTCCAGGT 
TTTGAAGGAG TCACCTGTGA GCGGAATATA GATGACTGTC CTAACCACAA GTGTCAGAAT 
GGAGGGGTTT GTGTGGATGG GGTCAATACC TACAACTGCC GCTGTCCCCC TCAGTGGACA 
GGACAGTTCT GCACAGAGGA TGTGGATGAG TGCCTGCTGC AGCCCAATGC CTGTCAGAAC 
GGGGGCACCT GTACCAACCG CAACGGGGGC TATGGCTGTG TATGTGTGAA TGGCTGGAGT 
GGGGACGACT GCAGTGAGAA TATCGATGAC TGTGCCTTCG CCTCCTGCAC CCCGGGCTCT 
ACCTGCATCG ATCGTGTGGC CTCCTTCTCT TGTATGTGCC CAGAAGGAAA GGCAGGTCTC 
TTATGTCATC TGGATGACGC GTGCATCAGC AACCCCTGCC ACAAGGGGGC GCTGTGTGAT 
ACCAACCCTC TGAATGGACA GTATATTTGC ACCTGCCCAC AAGGCTACAA AGGGAGTGAC 
TGTACAGAAG ATGTGGATGA GTGTGCCATG GCCAACAGCA ATCCTTGTGA GCATGCAGGA 
AAATGTGTGA ACACAGATGG TGCCTTCCAC TGTGAGTGTC TGAAGGGCTA TGCAGGACCT 
CGTTGTGAGA TGGACATCAA TGAGTGCCAC TCAGACCCCT GCAAGAATGA TGCCACCTGT 
TTGGATAAGA TTGGAGGCTT CACGTGTCTG TGCATGCCAG GTTTCAAAGG TGTGCATTGT 
GAGTTGGAGA TAAATGAATG TCAGAGCAAC CCTTGTGTGA ACAATGGACA GTGTGTGGAT 
AAAGTCAATC GCTTCCAGTG TCTGTGTCCG CCTGGATTCA CGGGACCAGT TTGCCAGATT 
GATATTGATG ACTGTTCTAG TACTCCGTGT CTGAATGGGG CAAAGTGTAT AGATCACCCG 
AATGGCTATG AGTGCCAGTG TGCCACAGGA TTCACTGGTG TGTTGTGTGA GGAGAACATT 
GACAACTGTG ACCCTGATCC TTGCCACCAC GGCCAGTGTC AGGATGGCAT TGATTCCTAC 
ACCTGCATTT GCAATCCGGG CTACATGGGC GCCATCTGCA GTGACCAGAT AGACGAATGC 
TACAGCAGCC CTTGCCTGAA TGAGGGTCGC TGCATCGACC TGGTGAATGG TTACCAGTGC 
AACTGCCAGC CAGGCACGTC AGGTGTTAAT TGTGAAATCA ATTTTGATGA CTGTGCAAGT 
AACCCGTGTG TCCATGGAGT CTGTATGGAT GGCGTTAATC GTTACAGCTG TGTCTGCTCA 
CCAGGATTCA CAGGGCAGAG ATGTAACATT GACATTGATG AGTGTGCCTC CAATCCCTGT 
CGCAAGGGTG CAACGTGCAT CAATGATGTG AATGGTTTCC GCTGTATCTG CCCTGAGGGC 
CCCCACCATC CCAGCTGCTA CTCACAGGTG AACGAGTGCC TGAGCAATCC CTGTGTCCAT 
GGAAACTGTA CTGGGGGCCT CACTGGATAC AAGTGCCTCT GTGATGCAGG CTGGGTTGGC 
ATCAACTGTG AAGTGGACAA AAATGAATGT CTTTCTAATC CATGCCAGAA CGGGGGAACT 
TGTGACAATC TGGTGAATGG ATACAGGTGC ACTTGCAAGA AGGGCTTTAA AGGCTATAAC 
TGCCAGGTGA ATATTGATGA ATGTGCTTCA AATCCATGTC TGAACCAAGG AACCTGCCTT 
GATGACGTAA GCGGTTATAC TTGCCACTGT GTGCTGCCAT ACACAGGCAA GAACTGTCAG 
ACCGTATTGG CTCCCTGTTC CCCAAACCCT TGTGAGAATG CTGGTGTTTG CAAAGAGGCA 
CCAAATTTTG AGAGTTACTC CTGCCTGTGT GCTCCTGGCT GGCAAGGTCA GCGGTGTACC 
ATCGACATTG ATGAGTGTGT CTCCAAGCCC TGCATGAACC ACGGTCTCTG CCATAACACC 
CAGGGCAGCT ACATGTGTGA ATGTCCTCCA GGCTTCAGTG GCATGGACTG TGAGGAGGAC 
ATTGATGACT GCCTTGCCAA TCCTTGCCAG AATGGAGGTT CCTGTGTAGA TGGAGTGAAT 
ACTTTCTCCT GCCTGTGCCT TCCGGGCTTC ACTGGGGATA AATGTCAGAC AGACATGAAT 
GAGTGTCTGA GTGAGCCCTG TAAGAACGGA GGAACCTGCT CTGACTATGT CAACAGTTAC 
ACCTGCAAGT GCCCGGCGGG ATTTGATGGA GTCCACTGTG AGAACAACAT TGACGAGTGC 
ACTGAGAGCT CCTGTTTCAA TGGTGGCACG TGTATCGATG GGATTAACTC CTTCTCTTGC 
TTGTGCCCTG TGGGCTTCAC TGGTTCCTTC TGCCTCCATG AGATCAATGA ATGCAACTCT 
CATCCATGCC TGAATGAAGG AGTATGTGTT GATGGCCTGG GTACCTACCG CTGTATCTGC 
CCATTGGGCT ACACTGGAAA AAACTGTCAG ACCCTGGTGA ACCTCTGTAG TCGGTCTCCA 
TGTAAAAACA AAGGTACCTG CATTCAGGAT AAAGCAGAGT CGCGGTGCCT GTGTCCGTCT 
GGATGGGCTG GTGCTTACTG CGATGTTCCC AGTGTCTCCT GTGAGGTGGC GGCCTCCCAC 
AGAGGGGTGC CCGTTGACCG CTTGTGCCAG CACTCAGGCG TCTGTATCAG TGCGGGCAAC 
TCGCATCACT GCCAGTGCCC CCTGGGCTAC ACCGGGAGCT ACTGTGAGGA CCAGCTTGAT 
GAGTGCTCGT CCAATCCCTG CCAGCACGGC GCCACCTGCC GGGACTTCAT TGGTGGATAC 
AGATGTGAGT GTGTCCCTGG ATATCAGGGT GTCAACTGCG AGTATGAAGT GGATGAGTGC 
CAGAACCAGC CATGCCAGAA TGGGGGCACC TGTATCGACC TTGTGAACCA CTTCAAGTGC 
TCCTGCCCAC CAGGCACTCG GGGCCTGCTT TGTGAAGAGA ACGTTGACGA CTGTGCCAGG 
GGTCCCCACT GCCTTAATGG TGGCCAGTGT GTAGACAGGA TTGGGGGCTA CAGTTGTCGC 
TGCTTGCCAG GCTTTGCTGG AGAACGCTGT GAAGGGGACA TCAACGAGTG CCTGTCCAAC 
CCCTGCAGCT CTGAGGGCAG CCTGGACTGC ATACAGCTCA CCAACGACTA CCTGTGCGTC 
TGCCGCAGCA CCTTCACTGG TCGTCACTGT GAAACGTTCG TCGACGTGTG TCCCCAGATG 
CCTTGCCTGA ACGGAGGCAC TTGTGCTGTG GCCAGCAACA TGCCCGATGG TTTCATCTGT 
CGTTGTCCCC CGGGCTTCTC TGGGGCACGA TGCCAGAGCA GCTGCGGACA GGTGAAGTGC 
AGGAGAGGGG AGCAATGTGT GCACACGGCC TCGGGCCCCC GCTGCTTCTG CCCCAACCCC 
CAGGACTGCG ACTCGGGCTG CGCCAGTAGC CCCTGCCAGC ACGGGGGGAG CTGCTACCCT 
CAGCGCCAGC CTCCTTACTA CTCGTGCCAG TGTGCCCCGC CGTTCCGGGG CAGCCACTGT 
GAACTCTACA CCGCCCCCAC CAGCACCCCT CCCGCCACCT GCCTGAGCCA GTATTGTGCT 
GACAAAGCTC GGGACGGCAT CTGTGATGAC GTGTGCAACA GCCATGCCTG CCAGTGGGAT 
GGGGGGGACT GTTCCCTCAC CATGGAGGAC CCCTGGGCCA ATTGCTCCTC CCCGCTGCCC 
TGCTGGGAGT ACATCAACAA CCAGTGCGAC GAGCTGTGCA ACACGGCCGA GTGCCTGTTT 
GACAACTTTG AATGCCAGAA GAGTAGCAAG ACATGCAAAT ATGACAAATA CTGTGCAGAC 
CACTACGAAG ACAACCATTG TGACCAGGGT TGCAACAGTG AGGAGTGTGG CTGGGATGGG 
CTGGACTGTG CTGCTGACCG GCCAGAAAAC CTGGCAGAGG GCACCCTGGT CATCGTGGTG 
CTGATGCCAC CTGAACAGCT GCTCCAGGAT GCCCGCAGAT TCTTGCGGGC ACTGGGCACC 
CTGCTCCACA CCAACCTCCG CATCAAACGG TCTGCCCAGG GGGAACTCAT GGTCTACCCC 
TATTATGGTG GGAAGTCAGC TGCCATGAAG AAGCAGAGAC TGACACGCAG GTCCCTTCCT 
GGAGATGAAG AACAGGAAGT GGCTGGCTCT CAGGTATTTC TGGAGATTGA CAACCGCCAG 
TGTGTTCAAG ACTCAGACCA GTGCTTTAAG AACACAGACG CAGCCGCAGC TCTTCTGGCT 
TCTCACGCCA TCCAGGGGAC CCTGTCGTAC CCACTTGTGT CTGTCGTCAG TGAATCTCTG 
ACCCCAAAAC GCACTCAGCT TCTCTATCTG CTTGCTGTTG CCGTTGTCAT TATCTTGTTT 
ATTATCCTGC TGGGGGTTAT CATGGCAAAA CGAAAGCGCA AGCATGGCTC CCTCTGGCTG 
CCTGAAGGTT TCACCCTTCG CCGTGACTCC AGCAACCACA AACGTCGTGA GCCAGTGGGC 
CAGGATGCTG TGGGACTCAA AAATCTTTCA GTGCAAGTCT CAGAGGCGAA CCTAATTGGT 
TCTGGAACAA GTGAACACTG GGTCGATGAT GAAGGGCCGC AGCCTAAGAA AGCCAAGGCT 
GAAGATGAGG CTTTGCTCTC AGAAGAAGAT GACCCCATCG ATCGGCGTCC ATGGACACAG 
CAGCACCTGG AAGCTGCAGA CATCCGTAGG ACACCATCGC TGGCTCTCAC CCCTCCACAG 
GCAGAGCAGG AGGTGGACGT GCTGGATGTG AACGTCCGCG GTCCAGATGG GTGCACCCCA 
CTGATGCTGG CTTCTCTCCG AGGAGGCAGC TCAGACATGA GTGATGAAGA TGAAGATGCA 
GAGGACTCTT CTGCCAACAT CATCACGGAC CTAGTCTACC AGGGTGCCAG CCTCCAAGCA 
CAGACAGACC GGACGGGTGA GATGGCCCTG CATCTCGCAG CCCGGTACTC AAGGGCAGAT 
GCTGCCAAGC GTCTCCTGGA TGCAGGTGCA GATGCCAATG CCCAGGACAA CATGGGCCGC 
TGCCCTCTCC ATGCTGCAGT GGCTGCTGAT GCCCAAGGTG TCTTCCAGAT ACTGATCCGC 
AACCGAGTAA CTGATCTAGA CGCAAGGATG AATGATGGCA CTACACCCCT GATCCTGGCT 
GCCCGCCTGG CAGTGGAGGG AATGGTGGCA GAACTGATCA ACTGCCAAGC AGATGTGAAT 
GCAGTGGATG ACCATGGAAA ATCTGCCCTT CACTGGGCAG CTGCTGTGAA TAATGTGGAG 
GCAACCCTTT TGCTGTTGAA AAATGGGGCC AACCGAGACA TGCAGGACAA CAAGGAGGAG 
ACACCCCTGT TTCTTGCTGC CCGGGAGGGG AGCTATGAAG CAGCCAAGAT CCTGTTAGAC 
CATTTTGCCA ATCGGGACAT CACAGACCAC ATGGATCGCC TTCCCCGGGA CGTGGCCCGG 
GACCGCATGC ACCATGACAT CGTGCGCCTG CTGGACGAGT ACAACGTGAC CCCCAGCCCT 
CCCGGCACAG TGCTGACTTC TGCGCTCTCA CCCGTCATCT GTGGGCCCAA CAGACCTTTT 
CTCAGTTTGA AGCATACCCC AATGGGCAAG AAGTCTAGAC GGCCTAACAC CAAGAGTACC 
GTGCCCACTA GCCTCCCTAA CCTTGCCAAG GAGGCCAAGG ATGCCAAGGG GAACAGGAGG 
AAGAAGTCTC TGAGTGACAA GGGCCAGCTG TCCGAGAGCT CAGTGACTCT GTCCCCCGTC 
GATTCCCTAG AATCTCCGCA CACATATGTT TCTGACACCA CCTCCTCTCC AATGATCACA 
TCCCCCGGAA TCTTACAGGC CTCCCCCAAC CCCATGCTGG CCACTGCCGC CCCCACTGCC 
CCAGTCCATG CACAGCATGC ACTGTCTTTC TCTAACCTTC ATGAGATGCA GCCCTTGGCT 
CATGGGGCCA ACACTGTGCT TCCCTCGGTG AGCCAGCTGC TGTCTCACCG CCACATTGTG 
CCCCCGGGCA GCAGCACCGC AGGGAGCTTG GGTAGGCTGC ATTCAGTCAC TGTCCCCGCC 
GACTGGATGA ACCGCGTGGA GATGAATGAG AGCCAGTACA ACGAGATGTT CGGTATGGTC 
CTGGCTCCAG CTGAGGGCAC CCACCCTGGC ATAGCTCCCC AGAGCAGGCC GCCCGAAGGG 
AAGCATTTAA CCACCCCACG AGAGCCCTTG CCCCCCATTG TGACTTTCCA GCTAATCCCC 
AAAGGCAGCA TTGCCCAACC AACTGGGGCT GCCCAGCCTC CGTCCAACTG CCCTCCAGCT 
GTTGCAGGCA CCCTGCCCAC CATGTATCAG ATTCCAGAAA TGGCTCGCTT GCCCAGTATG 
GCTTTTCCCA CTGCCATGAT GCCCCAGCAG GATGGGCAGG TTGCTCAGAC CATTCTCCCA 
GCCTATCACG CCTTCCCAGC CTCCGTGGGC AAGTATCCCA CACCCCCATC ACAGCATAGC 
TATGCTTCCT CCAACGCTGC TGAGAGAACG CCCAGTCACA GTGGTCACCT CCAGGGTGAG 
CATCCCTACC TGACACCGTC CCCAGAGTCT CCTGACCAGT GGTCAAGTTC ATCACCCCAC 
TCTGCTTCTG ACTGGTCAGA TGTGACCACC AGCCCCACTC CTGGGGGTGC TGGAGGAGGT 
CAGCGGGGAC CTGGGACACA CATGTCTGAG CCACCACGCA GCAACATGCA GGTCTATGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_024990004.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845772.1 Bos taurus notch 2 (NOTCH2), transcript variant X4, mRNA.

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CloneID	OBa233212
Clone ID Related Accession (Same CDS sequence)	XM_024990004.1
Accession Version	XM_024990004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACT GGAGGTGTCC AGAGGGCTTC TTGGGAGAAT ATTGTCAGCA TCGAGACCCC 
TGTGAGAAGA ACCGTTGCCA GAATGGTGGG ACGTGTGTGG CCCAGGCTAT GTTGGGGAAA 
GCCACGTGCC GCTGTGCCCC GGGCTTCACG GGCGAGGACT GCCAGCACTC GACTACTCAC 
CCCTGCTTTG TGTCTCACCC CTGCCTGAAT GGGGGCACCT GCCACGTGCT TAGCCGGGAC 
ACCTACGAGT GTACCTGCCA AGTCGGTTTT ACAGGTAAAC TGTGCCAGTG GACTGATGCC 
TGCCTGTCCC ACCCCTGTGC AAACGGAAGT ACCTGTACCA CTATGGCCAA CCAGTTCTCC 
TGCACATGCC TTGCAGGCTT CACAGGGCAG AAATGTGAGA CTGATGTCAA TGAGTGTGAC 
ATTCCAGGAC AGTGCCAGCA CGGTGGCACC TGCCTCAACC TCCCAGGCTC CTATCAGTGC 
CAATGCCCCC AGGGCTTCAC AGGCCAGCAC TGCGACAGTC CCTATGTGCC CTGCGCACCC 
TCCCCCTGTG TCAACGGAGG TACCTGCCGG CAGACCGGTG ACTTTACCTT TGAGTGCAGT 
TGCCTTCCAG GTTTTGAAGG AGTCACCTGT GAGCGGAATA TAGATGACTG TCCTAACCAC 
AAGTGTCAGA ATGGAGGGGT TTGTGTGGAT GGGGTCAATA CCTACAACTG CCGCTGTCCC 
CCTCAGTGGA CAGGACAGTT CTGCACAGAG GATGTGGATG AGTGCCTGCT GCAGCCCAAT 
GCCTGTCAGA ACGGGGGCAC CTGTACCAAC CGCAACGGGG GCTATGGCTG TGTATGTGTG 
AATGGCTGGA GTGGGGACGA CTGCAGTGAG AATATCGATG ACTGTGCCTT CGCCTCCTGC 
ACCCCGGGCT CTACCTGCAT CGATCGTGTG GCCTCCTTCT CTTGTATGTG CCCAGAAGGA 
AAGGCAGGTC TCTTATGTCA TCTGGATGAC GCGTGCATCA GCAACCCCTG CCACAAGGGG 
GCGCTGTGTG ATACCAACCC TCTGAATGGA CAGTATATTT GCACCTGCCC ACAAGGCTAC 
AAAGGGAGTG ACTGTACAGA AGATGTGGAT GAGTGTGCCA TGGCCAACAG CAATCCTTGT 
GAGCATGCAG GAAAATGTGT GAACACAGAT GGTGCCTTCC ACTGTGAGTG TCTGAAGGGC 
TATGCAGGAC CTCGTTGTGA GATGGACATC AATGAGTGCC ACTCAGACCC CTGCAAGAAT 
GATGCCACCT GTTTGGATAA GATTGGAGGC TTCACGTGTC TGTGCATGCC AGGTTTCAAA 
GGTGTGCATT GTGAGTTGGA GATAAATGAA TGTCAGAGCA ACCCTTGTGT GAACAATGGA 
CAGTGTGTGG ATAAAGTCAA TCGCTTCCAG TGTCTGTGTC CGCCTGGATT CACGGGACCA 
GTTTGCCAGA TTGATATTGA TGACTGTTCT AGTACTCCGT GTCTGAATGG GGCAAAGTGT 
ATAGATCACC CGAATGGCTA TGAGTGCCAG TGTGCCACAG GATTCACTGG TGTGTTGTGT 
GAGGAGAACA TTGACAACTG TGACCCTGAT CCTTGCCACC ACGGCCAGTG TCAGGATGGC 
ATTGATTCCT ACACCTGCAT TTGCAATCCG GGCTACATGG GCGCCATCTG CAGTGACCAG 
ATAGACGAAT GCTACAGCAG CCCTTGCCTG AATGAGGGTC GCTGCATCGA CCTGGTGAAT 
GGTTACCAGT GCAACTGCCA GCCAGGCACG TCAGGTGTTA ATTGTGAAAT CAATTTTGAT 
GACTGTGCAA GTAACCCGTG TGTCCATGGA GTCTGTATGG ATGGCGTTAA TCGTTACAGC 
TGTGTCTGCT CACCAGGATT CACAGGGCAG AGATGTAACA TTGACATTGA TGAGTGTGCC 
TCCAATCCCT GTCGCAAGGG TGCAACGTGC ATCAATGATG TGAATGGTTT CCGCTGTATC 
TGCCCTGAGG GCCCCCACCA TCCCAGCTGC TACTCACAGG TGAACGAGTG CCTGAGCAAT 
CCCTGTGTCC ATGGAAACTG TACTGGGGGC CTCACTGGAT ACAAGTGCCT CTGTGATGCA 
GGCTGGGTTG GCATCAACTG TGAAGTGGAC AAAAATGAAT GTCTTTCTAA TCCATGCCAG 
AACGGGGGAA CTTGTGACAA TCTGGTGAAT GGATACAGGT GCACTTGCAA GAAGGGCTTT 
AAAGGCTATA ACTGCCAGGT GAATATTGAT GAATGTGCTT CAAATCCATG TCTGAACCAA 
GGAACCTGCC TTGATGACGT AAGCGGTTAT ACTTGCCACT GTGTGCTGCC ATACACAGGC 
AAGAACTGTC AGACCGTATT GGCTCCCTGT TCCCCAAACC CTTGTGAGAA TGCTGGTGTT 
TGCAAAGAGG CACCAAATTT TGAGAGTTAC TCCTGCCTGT GTGCTCCTGG CTGGCAAGGT 
CAGCGGTGTA CCATCGACAT TGATGAGTGT GTCTCCAAGC CCTGCATGAA CCACGGTCTC 
TGCCATAACA CCCAGGGCAG CTACATGTGT GAATGTCCTC CAGGCTTCAG TGGCATGGAC 
TGTGAGGAGG ACATTGATGA CTGCCTTGCC AATCCTTGCC AGAATGGAGG TTCCTGTGTA 
GATGGAGTGA ATACTTTCTC CTGCCTGTGC CTTCCGGGCT TCACTGGGGA TAAATGTCAG 
ACAGACATGA ATGAGTGTCT GAGTGAGCCC TGTAAGAACG GAGGAACCTG CTCTGACTAT 
GTCAACAGTT ACACCTGCAA GTGCCCGGCG GGATTTGATG GAGTCCACTG TGAGAACAAC 
ATTGACGAGT GCACTGAGAG CTCCTGTTTC AATGGTGGCA CGTGTATCGA TGGGATTAAC 
TCCTTCTCTT GCTTGTGCCC TGTGGGCTTC ACTGGTTCCT TCTGCCTCCA TGAGATCAAT 
GAATGCAACT CTCATCCATG CCTGAATGAA GGAGTATGTG TTGATGGCCT GGGTACCTAC 
CGCTGTATCT GCCCATTGGG CTACACTGGA AAAAACTGTC AGACCCTGGT GAACCTCTGT 
AGTCGGTCTC CATGTAAAAA CAAAGGTACC TGCATTCAGG ATAAAGCAGA GTCGCGGTGC 
CTGTGTCCGT CTGGATGGGC TGGTGCTTAC TGCGATGTTC CCAGTGTCTC CTGTGAGGTG 
GCGGCCTCCC ACAGAGGGGT GCCCGTTGAC CGCTTGTGCC AGCACTCAGG CGTCTGTATC 
AGTGCGGGCA ACTCGCATCA CTGCCAGTGC CCCCTGGGCT ACACCGGGAG CTACTGTGAG 
GACCAGCTTG ATGAGTGCTC GTCCAATCCC TGCCAGCACG GCGCCACCTG CCGGGACTTC 
ATTGGTGGAT ACAGATGTGA GTGTGTCCCT GGATATCAGG GTGTCAACTG CGAGTATGAA 
GTGGATGAGT GCCAGAACCA GCCATGCCAG AATGGGGGCA CCTGTATCGA CCTTGTGAAC 
CACTTCAAGT GCTCCTGCCC ACCAGGCACT CGGGGCCTGC TTTGTGAAGA GAACGTTGAC 
GACTGTGCCA GGGGTCCCCA CTGCCTTAAT GGTGGCCAGT GTGTAGACAG GATTGGGGGC 
TACAGTTGTC GCTGCTTGCC AGGCTTTGCT GGAGAACGCT GTGAAGGGGA CATCAACGAG 
TGCCTGTCCA ACCCCTGCAG CTCTGAGGGC AGCCTGGACT GCATACAGCT CACCAACGAC 
TACCTGTGCG TCTGCCGCAG CACCTTCACT GGTCGTCACT GTGAAACGTT CGTCGACGTG 
TGTCCCCAGA TGCCTTGCCT GAACGGAGGC ACTTGTGCTG TGGCCAGCAA CATGCCCGAT 
GGTTTCATCT GTCGTTGTCC CCCGGGCTTC TCTGGGGCAC GATGCCAGAG CAGCTGCGGA 
CAGGTGAAGT GCAGGAGAGG GGAGCAATGT GTGCACACGG CCTCGGGCCC CCGCTGCTTC 
TGCCCCAACC CCCAGGACTG CGACTCGGGC TGCGCCAGTA GCCCCTGCCA GCACGGGGGG 
AGCTGCTACC CTCAGCGCCA GCCTCCTTAC TACTCGTGCC AGTGTGCCCC GCCGTTCCGG 
GGCAGCCACT GTGAACTCTA CACCGCCCCC ACCAGCACCC CTCCCGCCAC CTGCCTGAGC 
CAGTATTGTG CTGACAAAGC TCGGGACGGC ATCTGTGATG ACGTGTGCAA CAGCCATGCC 
TGCCAGTGGG ATGGGGGGGA CTGTTCCCTC ACCATGGAGG ACCCCTGGGC CAATTGCTCC 
TCCCCGCTGC CCTGCTGGGA GTACATCAAC AACCAGTGCG ACGAGCTGTG CAACACGGCC 
GAGTGCCTGT TTGACAACTT TGAATGCCAG AAGAGTAGCA AGACATGCAA ATATGACAAA 
TACTGTGCAG ACCACTACGA AGACAACCAT TGTGACCAGG GTTGCAACAG TGAGGAGTGT 
GGCTGGGATG GGCTGGACTG TGCTGCTGAC CGGCCAGAAA ACCTGGCAGA GGGCACCCTG 
GTCATCGTGG TGCTGATGCC ACCTGAACAG CTGCTCCAGG ATGCCCGCAG ATTCTTGCGG 
GCACTGGGCA CCCTGCTCCA CACCAACCTC CGCATCAAAC GGTCTGCCCA GGGGGAACTC 
ATGGTCTACC CCTATTATGG TGGGAAGTCA GCTGCCATGA AGAAGCAGAG ACTGACACGC 
AGGTCCCTTC CTGGAGATGA AGAACAGGAA GTGGCTGGCT CTCAGGTATT TCTGGAGATT 
GACAACCGCC AGTGTGTTCA AGACTCAGAC CAGTGCTTTA AGAACACAGA CGCAGCCGCA 
GCTCTTCTGG CTTCTCACGC CATCCAGGGG ACCCTGTCGT ACCCACTTGT GTCTGTCGTC 
AGTGAATCTC TGACCCCAAA ACGCACTCAG CTTCTCTATC TGCTTGCTGT TGCCGTTGTC 
ATTATCTTGT TTATTATCCT GCTGGGGGTT ATCATGGCAA AACGAAAGCG CAAGCATGGC 
TCCCTCTGGC TGCCTGAAGG TTTCACCCTT CGCCGTGACT CCAGCAACCA CAAACGTCGT 
GAGCCAGTGG GCCAGGATGC TGTGGGACTC AAAAATCTTT CAGTGCAAGT CTCAGAGGCG 
AACCTAATTG GTTCTGGAAC AAGTGAACAC TGGGTCGATG ATGAAGGGCC GCAGCCTAAG 
AAAGCCAAGG CTGAAGATGA GGCTTTGCTC TCAGAAGAAG ATGACCCCAT CGATCGGCGT 
CCATGGACAC AGCAGCACCT GGAAGCTGCA GACATCCGTA GGACACCATC GCTGGCTCTC 
ACCCCTCCAC AGGCAGAGCA GGAGGTGGAC GTGCTGGATG TGAACGTCCG CGGTCCAGAT 
GGGTGCACCC CACTGATGCT GGCTTCTCTC CGAGGAGGCA GCTCAGACAT GAGTGATGAA 
GATGAAGATG CAGAGGACTC TTCTGCCAAC ATCATCACGG ACCTAGTCTA CCAGGGTGCC 
AGCCTCCAAG CACAGACAGA CCGGACGGGT GAGATGGCCC TGCATCTCGC AGCCCGGTAC 
TCAAGGGCAG ATGCTGCCAA GCGTCTCCTG GATGCAGGTG CAGATGCCAA TGCCCAGGAC 
AACATGGGCC GCTGCCCTCT CCATGCTGCA GTGGCTGCTG ATGCCCAAGG TGTCTTCCAG 
ATACTGATCC GCAACCGAGT AACTGATCTA GACGCAAGGA TGAATGATGG CACTACACCC 
CTGATCCTGG CTGCCCGCCT GGCAGTGGAG GGAATGGTGG CAGAACTGAT CAACTGCCAA 
GCAGATGTGA ATGCAGTGGA TGACCATGGA AAATCTGCCC TTCACTGGGC AGCTGCTGTG 
AATAATGTGG AGGCAACCCT TTTGCTGTTG AAAAATGGGG CCAACCGAGA CATGCAGGAC 
AACAAGGAGG AGACACCCCT GTTTCTTGCT GCCCGGGAGG GGAGCTATGA AGCAGCCAAG 
ATCCTGTTAG ACCATTTTGC CAATCGGGAC ATCACAGACC ACATGGATCG CCTTCCCCGG 
GACGTGGCCC GGGACCGCAT GCACCATGAC ATCGTGCGCC TGCTGGACGA GTACAACGTG 
ACCCCCAGCC CTCCCGGCAC AGTGCTGACT TCTGCGCTCT CACCCGTCAT CTGTGGGCCC 
AACAGACCTT TTCTCAGTTT GAAGCATACC CCAATGGGCA AGAAGTCTAG ACGGCCTAAC 
ACCAAGAGTA CCGTGCCCAC TAGCCTCCCT AACCTTGCCA AGGAGGCCAA GGATGCCAAG 
GGGAACAGGA GGAAGAAGTC TCTGAGTGAC AAGGGCCAGC TGTCCGAGAG CTCAGTGACT 
CTGTCCCCCG TCGATTCCCT AGAATCTCCG CACACATATG TTTCTGACAC CACCTCCTCT 
CCAATGATCA CATCCCCCGG AATCTTACAG GCCTCCCCCA ACCCCATGCT GGCCACTGCC 
GCCCCCACTG CCCCAGTCCA TGCACAGCAT GCACTGTCTT TCTCTAACCT TCATGAGATG 
CAGCCCTTGG CTCATGGGGC CAACACTGTG CTTCCCTCGG TGAGCCAGCT GCTGTCTCAC 
CGCCACATTG TGCCCCCGGG CAGCAGCACC GCAGGGAGCT TGGGTAGGCT GCATTCAGTC 
ACTGTCCCCG CCGACTGGAT GAACCGCGTG GAGATGAATG AGAGCCAGTA CAACGAGATG 
TTCGGTATGG TCCTGGCTCC AGCTGAGGGC ACCCACCCTG GCATAGCTCC CCAGAGCAGG 
CCGCCCGAAG GGAAGCATTT AACCACCCCA CGAGAGCCCT TGCCCCCCAT TGTGACTTTC 
CAGCTAATCC CCAAAGGCAG CATTGCCCAA CCAACTGGGG CTGCCCAGCC TCCGTCCAAC 
TGCCCTCCAG CTGTTGCAGG CACCCTGCCC ACCATGTATC AGATTCCAGA AATGGCTCGC 
TTGCCCAGTA TGGCTTTTCC CACTGCCATG ATGCCCCAGC AGGATGGGCA GGTTGCTCAG 
ACCATTCTCC CAGCCTATCA CGCCTTCCCA GCCTCCGTGG GCAAGTATCC CACACCCCCA 
TCACAGCATA GCTATGCTTC CTCCAACGCT GCTGAGAGAA CGCCCAGTCA CAGTGGTCAC 
CTCCAGGGTG AGCATCCCTA CCTGACACCG TCCCCAGAGT CTCCTGACCA GTGGTCAAGT 
TCATCACCCC ACTCTGCTTC TGACTGGTCA GATGTGACCA CCAGCCCCAC TCCTGGGGGT 
GCTGGAGGAG GTCAGCGGGG ACCTGGGACA CACATGTCTG AGCCACCACG CAGCAACATG 
CAGGTCTATG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845772.1
CDS	16..7290
Translation	MFNWRCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKRTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_024990004.1
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990004.1

ORF Insert Sequence:

ATGTTCAACT GGAGGTGTCC AGAGGGCTTC TTGGGAGAAT ATTGTCAGCA TCGAGACCCC 
TGTGAGAAGA ACCGTTGCCA GAATGGTGGG ACGTGTGTGG CCCAGGCTAT GTTGGGGAAA 
GCCACGTGCC GCTGTGCCCC GGGCTTCACG GGCGAGGACT GCCAGCACTC GACTACTCAC 
CCCTGCTTTG TGTCTCACCC CTGCCTGAAT GGGGGCACCT GCCACGTGCT TAGCCGGGAC 
ACCTACGAGT GTACCTGCCA AGTCGGTTTT ACAGGTAAAC TGTGCCAGTG GACTGATGCC 
TGCCTGTCCC ACCCCTGTGC AAACGGAAGT ACCTGTACCA CTATGGCCAA CCAGTTCTCC 
TGCACATGCC TTGCAGGCTT CACAGGGCAG AAATGTGAGA CTGATGTCAA TGAGTGTGAC 
ATTCCAGGAC AGTGCCAGCA CGGTGGCACC TGCCTCAACC TCCCAGGCTC CTATCAGTGC 
CAATGCCCCC AGGGCTTCAC AGGCCAGCAC TGCGACAGTC CCTATGTGCC CTGCGCACCC 
TCCCCCTGTG TCAACGGAGG TACCTGCCGG CAGACCGGTG ACTTTACCTT TGAGTGCAGT 
TGCCTTCCAG GTTTTGAAGG AGTCACCTGT GAGCGGAATA TAGATGACTG TCCTAACCAC 
AAGTGTCAGA ATGGAGGGGT TTGTGTGGAT GGGGTCAATA CCTACAACTG CCGCTGTCCC 
CCTCAGTGGA CAGGACAGTT CTGCACAGAG GATGTGGATG AGTGCCTGCT GCAGCCCAAT 
GCCTGTCAGA ACGGGGGCAC CTGTACCAAC CGCAACGGGG GCTATGGCTG TGTATGTGTG 
AATGGCTGGA GTGGGGACGA CTGCAGTGAG AATATCGATG ACTGTGCCTT CGCCTCCTGC 
ACCCCGGGCT CTACCTGCAT CGATCGTGTG GCCTCCTTCT CTTGTATGTG CCCAGAAGGA 
AAGGCAGGTC TCTTATGTCA TCTGGATGAC GCGTGCATCA GCAACCCCTG CCACAAGGGG 
GCGCTGTGTG ATACCAACCC TCTGAATGGA CAGTATATTT GCACCTGCCC ACAAGGCTAC 
AAAGGGAGTG ACTGTACAGA AGATGTGGAT GAGTGTGCCA TGGCCAACAG CAATCCTTGT 
GAGCATGCAG GAAAATGTGT GAACACAGAT GGTGCCTTCC ACTGTGAGTG TCTGAAGGGC 
TATGCAGGAC CTCGTTGTGA GATGGACATC AATGAGTGCC ACTCAGACCC CTGCAAGAAT 
GATGCCACCT GTTTGGATAA GATTGGAGGC TTCACGTGTC TGTGCATGCC AGGTTTCAAA 
GGTGTGCATT GTGAGTTGGA GATAAATGAA TGTCAGAGCA ACCCTTGTGT GAACAATGGA 
CAGTGTGTGG ATAAAGTCAA TCGCTTCCAG TGTCTGTGTC CGCCTGGATT CACGGGACCA 
GTTTGCCAGA TTGATATTGA TGACTGTTCT AGTACTCCGT GTCTGAATGG GGCAAAGTGT 
ATAGATCACC CGAATGGCTA TGAGTGCCAG TGTGCCACAG GATTCACTGG TGTGTTGTGT 
GAGGAGAACA TTGACAACTG TGACCCTGAT CCTTGCCACC ACGGCCAGTG TCAGGATGGC 
ATTGATTCCT ACACCTGCAT TTGCAATCCG GGCTACATGG GCGCCATCTG CAGTGACCAG 
ATAGACGAAT GCTACAGCAG CCCTTGCCTG AATGAGGGTC GCTGCATCGA CCTGGTGAAT 
GGTTACCAGT GCAACTGCCA GCCAGGCACG TCAGGTGTTA ATTGTGAAAT CAATTTTGAT 
GACTGTGCAA GTAACCCGTG TGTCCATGGA GTCTGTATGG ATGGCGTTAA TCGTTACAGC 
TGTGTCTGCT CACCAGGATT CACAGGGCAG AGATGTAACA TTGACATTGA TGAGTGTGCC 
TCCAATCCCT GTCGCAAGGG TGCAACGTGC ATCAATGATG TGAATGGTTT CCGCTGTATC 
TGCCCTGAGG GCCCCCACCA TCCCAGCTGC TACTCACAGG TGAACGAGTG CCTGAGCAAT 
CCCTGTGTCC ATGGAAACTG TACTGGGGGC CTCACTGGAT ACAAGTGCCT CTGTGATGCA 
GGCTGGGTTG GCATCAACTG TGAAGTGGAC AAAAATGAAT GTCTTTCTAA TCCATGCCAG 
AACGGGGGAA CTTGTGACAA TCTGGTGAAT GGATACAGGT GCACTTGCAA GAAGGGCTTT 
AAAGGCTATA ACTGCCAGGT GAATATTGAT GAATGTGCTT CAAATCCATG TCTGAACCAA 
GGAACCTGCC TTGATGACGT AAGCGGTTAT ACTTGCCACT GTGTGCTGCC ATACACAGGC 
AAGAACTGTC AGACCGTATT GGCTCCCTGT TCCCCAAACC CTTGTGAGAA TGCTGGTGTT 
TGCAAAGAGG CACCAAATTT TGAGAGTTAC TCCTGCCTGT GTGCTCCTGG CTGGCAAGGT 
CAGCGGTGTA CCATCGACAT TGATGAGTGT GTCTCCAAGC CCTGCATGAA CCACGGTCTC 
TGCCATAACA CCCAGGGCAG CTACATGTGT GAATGTCCTC CAGGCTTCAG TGGCATGGAC 
TGTGAGGAGG ACATTGATGA CTGCCTTGCC AATCCTTGCC AGAATGGAGG TTCCTGTGTA 
GATGGAGTGA ATACTTTCTC CTGCCTGTGC CTTCCGGGCT TCACTGGGGA TAAATGTCAG 
ACAGACATGA ATGAGTGTCT GAGTGAGCCC TGTAAGAACG GAGGAACCTG CTCTGACTAT 
GTCAACAGTT ACACCTGCAA GTGCCCGGCG GGATTTGATG GAGTCCACTG TGAGAACAAC 
ATTGACGAGT GCACTGAGAG CTCCTGTTTC AATGGTGGCA CGTGTATCGA TGGGATTAAC 
TCCTTCTCTT GCTTGTGCCC TGTGGGCTTC ACTGGTTCCT TCTGCCTCCA TGAGATCAAT 
GAATGCAACT CTCATCCATG CCTGAATGAA GGAGTATGTG TTGATGGCCT GGGTACCTAC 
CGCTGTATCT GCCCATTGGG CTACACTGGA AAAAACTGTC AGACCCTGGT GAACCTCTGT 
AGTCGGTCTC CATGTAAAAA CAAAGGTACC TGCATTCAGG ATAAAGCAGA GTCGCGGTGC 
CTGTGTCCGT CTGGATGGGC TGGTGCTTAC TGCGATGTTC CCAGTGTCTC CTGTGAGGTG 
GCGGCCTCCC ACAGAGGGGT GCCCGTTGAC CGCTTGTGCC AGCACTCAGG CGTCTGTATC 
AGTGCGGGCA ACTCGCATCA CTGCCAGTGC CCCCTGGGCT ACACCGGGAG CTACTGTGAG 
GACCAGCTTG ATGAGTGCTC GTCCAATCCC TGCCAGCACG GCGCCACCTG CCGGGACTTC 
ATTGGTGGAT ACAGATGTGA GTGTGTCCCT GGATATCAGG GTGTCAACTG CGAGTATGAA 
GTGGATGAGT GCCAGAACCA GCCATGCCAG AATGGGGGCA CCTGTATCGA CCTTGTGAAC 
CACTTCAAGT GCTCCTGCCC ACCAGGCACT CGGGGCCTGC TTTGTGAAGA GAACGTTGAC 
GACTGTGCCA GGGGTCCCCA CTGCCTTAAT GGTGGCCAGT GTGTAGACAG GATTGGGGGC 
TACAGTTGTC GCTGCTTGCC AGGCTTTGCT GGAGAACGCT GTGAAGGGGA CATCAACGAG 
TGCCTGTCCA ACCCCTGCAG CTCTGAGGGC AGCCTGGACT GCATACAGCT CACCAACGAC 
TACCTGTGCG TCTGCCGCAG CACCTTCACT GGTCGTCACT GTGAAACGTT CGTCGACGTG 
TGTCCCCAGA TGCCTTGCCT GAACGGAGGC ACTTGTGCTG TGGCCAGCAA CATGCCCGAT 
GGTTTCATCT GTCGTTGTCC CCCGGGCTTC TCTGGGGCAC GATGCCAGAG CAGCTGCGGA 
CAGGTGAAGT GCAGGAGAGG GGAGCAATGT GTGCACACGG CCTCGGGCCC CCGCTGCTTC 
TGCCCCAACC CCCAGGACTG CGACTCGGGC TGCGCCAGTA GCCCCTGCCA GCACGGGGGG 
AGCTGCTACC CTCAGCGCCA GCCTCCTTAC TACTCGTGCC AGTGTGCCCC GCCGTTCCGG 
GGCAGCCACT GTGAACTCTA CACCGCCCCC ACCAGCACCC CTCCCGCCAC CTGCCTGAGC 
CAGTATTGTG CTGACAAAGC TCGGGACGGC ATCTGTGATG ACGTGTGCAA CAGCCATGCC 
TGCCAGTGGG ATGGGGGGGA CTGTTCCCTC ACCATGGAGG ACCCCTGGGC CAATTGCTCC 
TCCCCGCTGC CCTGCTGGGA GTACATCAAC AACCAGTGCG ACGAGCTGTG CAACACGGCC 
GAGTGCCTGT TTGACAACTT TGAATGCCAG AAGAGTAGCA AGACATGCAA ATATGACAAA 
TACTGTGCAG ACCACTACGA AGACAACCAT TGTGACCAGG GTTGCAACAG TGAGGAGTGT 
GGCTGGGATG GGCTGGACTG TGCTGCTGAC CGGCCAGAAA ACCTGGCAGA GGGCACCCTG 
GTCATCGTGG TGCTGATGCC ACCTGAACAG CTGCTCCAGG ATGCCCGCAG ATTCTTGCGG 
GCACTGGGCA CCCTGCTCCA CACCAACCTC CGCATCAAAC GGTCTGCCCA GGGGGAACTC 
ATGGTCTACC CCTATTATGG TGGGAAGTCA GCTGCCATGA AGAAGCAGAG ACTGACACGC 
AGGTCCCTTC CTGGAGATGA AGAACAGGAA GTGGCTGGCT CTCAGGTATT TCTGGAGATT 
GACAACCGCC AGTGTGTTCA AGACTCAGAC CAGTGCTTTA AGAACACAGA CGCAGCCGCA 
GCTCTTCTGG CTTCTCACGC CATCCAGGGG ACCCTGTCGT ACCCACTTGT GTCTGTCGTC 
AGTGAATCTC TGACCCCAAA ACGCACTCAG CTTCTCTATC TGCTTGCTGT TGCCGTTGTC 
ATTATCTTGT TTATTATCCT GCTGGGGGTT ATCATGGCAA AACGAAAGCG CAAGCATGGC 
TCCCTCTGGC TGCCTGAAGG TTTCACCCTT CGCCGTGACT CCAGCAACCA CAAACGTCGT 
GAGCCAGTGG GCCAGGATGC TGTGGGACTC AAAAATCTTT CAGTGCAAGT CTCAGAGGCG 
AACCTAATTG GTTCTGGAAC AAGTGAACAC TGGGTCGATG ATGAAGGGCC GCAGCCTAAG 
AAAGCCAAGG CTGAAGATGA GGCTTTGCTC TCAGAAGAAG ATGACCCCAT CGATCGGCGT 
CCATGGACAC AGCAGCACCT GGAAGCTGCA GACATCCGTA GGACACCATC GCTGGCTCTC 
ACCCCTCCAC AGGCAGAGCA GGAGGTGGAC GTGCTGGATG TGAACGTCCG CGGTCCAGAT 
GGGTGCACCC CACTGATGCT GGCTTCTCTC CGAGGAGGCA GCTCAGACAT GAGTGATGAA 
GATGAAGATG CAGAGGACTC TTCTGCCAAC ATCATCACGG ACCTAGTCTA CCAGGGTGCC 
AGCCTCCAAG CACAGACAGA CCGGACGGGT GAGATGGCCC TGCATCTCGC AGCCCGGTAC 
TCAAGGGCAG ATGCTGCCAA GCGTCTCCTG GATGCAGGTG CAGATGCCAA TGCCCAGGAC 
AACATGGGCC GCTGCCCTCT CCATGCTGCA GTGGCTGCTG ATGCCCAAGG TGTCTTCCAG 
ATACTGATCC GCAACCGAGT AACTGATCTA GACGCAAGGA TGAATGATGG CACTACACCC 
CTGATCCTGG CTGCCCGCCT GGCAGTGGAG GGAATGGTGG CAGAACTGAT CAACTGCCAA 
GCAGATGTGA ATGCAGTGGA TGACCATGGA AAATCTGCCC TTCACTGGGC AGCTGCTGTG 
AATAATGTGG AGGCAACCCT TTTGCTGTTG AAAAATGGGG CCAACCGAGA CATGCAGGAC 
AACAAGGAGG AGACACCCCT GTTTCTTGCT GCCCGGGAGG GGAGCTATGA AGCAGCCAAG 
ATCCTGTTAG ACCATTTTGC CAATCGGGAC ATCACAGACC ACATGGATCG CCTTCCCCGG 
GACGTGGCCC GGGACCGCAT GCACCATGAC ATCGTGCGCC TGCTGGACGA GTACAACGTG 
ACCCCCAGCC CTCCCGGCAC AGTGCTGACT TCTGCGCTCT CACCCGTCAT CTGTGGGCCC 
AACAGACCTT TTCTCAGTTT GAAGCATACC CCAATGGGCA AGAAGTCTAG ACGGCCTAAC 
ACCAAGAGTA CCGTGCCCAC TAGCCTCCCT AACCTTGCCA AGGAGGCCAA GGATGCCAAG 
GGGAACAGGA GGAAGAAGTC TCTGAGTGAC AAGGGCCAGC TGTCCGAGAG CTCAGTGACT 
CTGTCCCCCG TCGATTCCCT AGAATCTCCG CACACATATG TTTCTGACAC CACCTCCTCT 
CCAATGATCA CATCCCCCGG AATCTTACAG GCCTCCCCCA ACCCCATGCT GGCCACTGCC 
GCCCCCACTG CCCCAGTCCA TGCACAGCAT GCACTGTCTT TCTCTAACCT TCATGAGATG 
CAGCCCTTGG CTCATGGGGC CAACACTGTG CTTCCCTCGG TGAGCCAGCT GCTGTCTCAC 
CGCCACATTG TGCCCCCGGG CAGCAGCACC GCAGGGAGCT TGGGTAGGCT GCATTCAGTC 
ACTGTCCCCG CCGACTGGAT GAACCGCGTG GAGATGAATG AGAGCCAGTA CAACGAGATG 
TTCGGTATGG TCCTGGCTCC AGCTGAGGGC ACCCACCCTG GCATAGCTCC CCAGAGCAGG 
CCGCCCGAAG GGAAGCATTT AACCACCCCA CGAGAGCCCT TGCCCCCCAT TGTGACTTTC 
CAGCTAATCC CCAAAGGCAG CATTGCCCAA CCAACTGGGG CTGCCCAGCC TCCGTCCAAC 
TGCCCTCCAG CTGTTGCAGG CACCCTGCCC ACCATGTATC AGATTCCAGA AATGGCTCGC 
TTGCCCAGTA TGGCTTTTCC CACTGCCATG ATGCCCCAGC AGGATGGGCA GGTTGCTCAG 
ACCATTCTCC CAGCCTATCA CGCCTTCCCA GCCTCCGTGG GCAAGTATCC CACACCCCCA 
TCACAGCATA GCTATGCTTC CTCCAACGCT GCTGAGAGAA CGCCCAGTCA CAGTGGTCAC 
CTCCAGGGTG AGCATCCCTA CCTGACACCG TCCCCAGAGT CTCCTGACCA GTGGTCAAGT 
TCATCACCCC ACTCTGCTTC TGACTGGTCA GATGTGACCA CCAGCCCCAC TCCTGGGGGT 
GCTGGAGGAG GTCAGCGGGG ACCTGGGACA CACATGTCTG AGCCACCACG CAGCAACATG 
CAGGTCTATG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_015462612.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318098.1 Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

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CloneID	OBa89538
Clone ID Related Accession (Same CDS sequence)	XM_015462612.1 , XM_015463442.1
Accession Version	XM_015462612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACCCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTCCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318098.1
CDS	819..8207
Translation	MANSPQPFTTSIYSATLQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKPTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_015462612.1
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462612.1

ORF Insert Sequence:

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACCCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTCCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_015463442.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318928.1 Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

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CloneID	OBa89538
Clone ID Related Accession (Same CDS sequence)	XM_015462612.1 , XM_015463442.1
Accession Version	XM_015463442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACCCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTCCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318928.1
CDS	819..8207
Translation	MANSPQPFTTSIYSATLQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKPTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_015463442.1
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463442.1

ORF Insert Sequence:

ATGGCTAACT CACCTCAACC CTTCACAACT TCAATATATT CTGCCACATT GCAATGTCGG 
GATGGCTATG AGCCTTGTGT AAATGAAGGA ATATGTGTTA CCTACCACAA CGGTACAGGA 
TACTGCAAGT GTCCAGAGGG CTTCTTGGGA GAATATTGTC AGCATCGAGA CCCCTGTGAG 
AAGAACCGTT GCCAGAATGG TGGGACGTGT GTGGCCCAGG CTATGTTGGG GAAAGCCACG 
TGCCGCTGTG CCCCGGGCTT CACGGGCGAG GACTGCCAGC ACTCGACTAC TCACCCCTGC 
TTTGTGTCTC ACCCCTGCCT GAATGGGGGC ACCTGCCACG TGCTTAGCCG GGACACCTAC 
GAGTGTACCT GCCAAGTCGG TTTTACAGGT AAACTGTGCC AGTGGACTGA TGCCTGCCTG 
TCCCACCCCT GTGCAAACGG AAGTACCTGT ACCACTATGG CCAACCAGTT CTCCTGCACA 
TGCCTTGCAG GCTTCACAGG GCAGAAATGT GAGACTGATG TCAATGAGTG TGACATTCCA 
GGACAGTGCC AGCACGGTGG CACCTGCCTC AACCTCCCAG GCTCCTATCA GTGCCAATGC 
CCCCAGGGCT TCACAGGCCA GCACTGCGAC AGTCCCTATG TGCCCTGCGC ACCCTCCCCC 
TGTGTCAACG GAGGTACCTG CCGGCAGACC GGTGACTTTA CCTTTGAGTG CAGTTGCCTT 
CCAGGTTTTG AAGGAGTCAC CTGTGAGCGG AATATAGATG ACTGTCCTAA CCACAAGTGT 
CAGAATGGAG GGGTTTGTGT GGATGGGGTC AATACCTACA ACTGCCGCTG TCCCCCTCAG 
TGGACAGGAC AGTTCTGCAC AGAGGATGTG GATGAGTGCC TGCTGCAGCC CAATGCCTGT 
CAGAACGGGG GCACCTGTAC CAACCGCAAC GGGGGCTATG GCTGTGTATG TGTGAATGGC 
TGGAGTGGGG ACGACTGCAG TGAGAATATC GATGACTGTG CCTTCGCCTC CTGCACCCCG 
GGCTCTACCT GCATCGATCG TGTGGCCTCC TTCTCTTGTA TGTGCCCAGA AGGAAAGGCA 
GGTCTCTTAT GTCATCTGGA TGACGCGTGC ATCAGCAACC CCTGCCACAA GGGGGCGCTG 
TGTGATACCA ACCCTCTGAA TGGACAGTAT ATTTGCACCT GCCCACAAGG CTACAAAGGG 
AGTGACTGTA CAGAAGATGT GGATGAGTGT GCCATGGCCA ACAGCAATCC TTGTGAGCAT 
GCAGGAAAAT GTGTGAACAC AGATGGTGCC TTCCACTGTG AGTGTCTGAA GGGCTATGCA 
GGACCTCGTT GTGAGATGGA CATCAATGAG TGCCACTCAG ACCCCTGCAA GAATGATGCC 
ACCTGTTTGG ATAAGATTGG AGGCTTCACG TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG 
CATTGTGAGT TGGAGATAAA TGAATGTCAG AGCAACCCTT GTGTGAACAA TGGACAGTGT 
GTGGATAAAG TCAATCGCTT CCAGTGTCTG TGTCCGCCTG GATTCACGGG ACCAGTTTGC 
CAGATTGATA TTGATGACTG TTCTAGTACT CCGTGTCTGA ATGGGGCAAA GTGTATAGAT 
CACCCGAATG GCTATGAGTG CCAGTGTGCC ACAGGATTCA CTGGTGTGTT GTGTGAGGAG 
AACATTGACA ACTGTGACCC TGATCCTTGC CACCACGGCC AGTGTCAGGA TGGCATTGAT 
TCCTACACCT GCATTTGCAA TCCGGGCTAC ATGGGCGCCA TCTGCAGTGA CCAGATAGAC 
GAATGCTACA GCAGCCCTTG CCTGAATGAG GGTCGCTGCA TCGACCTGGT GAATGGTTAC 
CAGTGCAACT GCCAGCCAGG CACGTCAGGT GTTAATTGTG AAATCAATTT TGATGACTGT 
GCAAGTAACC CGTGTGTCCA TGGAGTCTGT ATGGATGGCG TTAATCGTTA CAGCTGTGTC 
TGCTCACCAG GATTCACAGG GCAGAGATGT AACATTGACA TTGATGAGTG TGCCTCCAAT 
CCCTGTCGCA AGGGTGCAAC GTGCATCAAT GATGTGAATG GTTTCCGCTG TATCTGCCCT 
GAGGGCCCCC ACCATCCCAG CTGCTACTCA CAGGTGAACG AGTGCCTGAG CAATCCCTGT 
GTCCATGGAA ACTGTACTGG GGGCCTCACT GGATACAAGT GCCTCTGTGA TGCAGGCTGG 
GTTGGCATCA ACTGTGAAGT GGACAAAAAT GAATGTCTTT CTAATCCATG CCAGAACGGG 
GGAACTTGTG ACAATCTGGT GAATGGATAC AGGTGCACTT GCAAGAAGGG CTTTAAAGGC 
TATAACTGCC AGGTGAATAT TGATGAATGT GCTTCAAATC CATGTCTGAA CCAAGGAACC 
TGCCTTGATG ACGTAAGCGG TTATACTTGC CACTGTGTGC TGCCATACAC AGGCAAGAAC 
TGTCAGACCG TATTGGCTCC CTGTTCCCCA AACCCTTGTG AGAATGCTGG TGTTTGCAAA 
GAGGCACCAA ATTTTGAGAG TTACTCCTGC CTGTGTGCTC CTGGCTGGCA AGGTCAGCGG 
TGTACCATCG ACATTGATGA GTGTGTCTCC AAGCCCTGCA TGAACCACGG TCTCTGCCAT 
AACACCCAGG GCAGCTACAT GTGTGAATGT CCTCCAGGCT TCAGTGGCAT GGACTGTGAG 
GAGGACATTG ATGACTGCCT TGCCAATCCT TGCCAGAATG GAGGTTCCTG TGTAGATGGA 
GTGAATACTT TCTCCTGCCT GTGCCTTCCG GGCTTCACTG GGGATAAATG TCAGACAGAC 
ATGAATGAGT GTCTGAGTGA GCCCTGTAAG AACGGAGGAA CCTGCTCTGA CTATGTCAAC 
AGTTACACCT GCAAGTGCCC GGCGGGATTT GATGGAGTCC ACTGTGAGAA CAACATTGAC 
GAGTGCACTG AGAGCTCCTG TTTCAATGGT GGCACGTGTA TCGATGGGAT TAACTCCTTC 
TCTTGCTTGT GCCCTGTGGG CTTCACTGGT TCCTTCTGCC TCCATGAGAT CAATGAATGC 
AACTCTCATC CATGCCTGAA TGAAGGAGTA TGTGTTGATG GCCTGGGTAC CTACCGCTGT 
ATCTGCCCAT TGGGCTACAC TGGAAAAAAC TGTCAGACCC TGGTGAACCT CTGTAGTCGG 
TCTCCATGTA AAAACAAAGG TACCTGCATT CAGGATAAAG CAGAGTCGCG GTGCCTGTGT 
CCGTCTGGAT GGGCTGGTGC TTACTGCGAT GTTCCCAGTG TCTCCTGTGA GGTGGCGGCC 
TCCCACAGAG GGGTGCCCGT TGACCGCTTG TGCCAGCACT CAGGCGTCTG TATCAGTGCG 
GGCAACTCGC ATCACTGCCA GTGCCCCCTG GGCTACACCG GGAGCTACTG TGAGGACCAG 
CTTGATGAGT GCTCGTCCAA TCCCTGCCAG CACGGCGCCA CCTGCCGGGA CTTCATTGGT 
GGATACAGAT GTGAGTGTGT CCCTGGATAT CAGGGTGTCA ACTGCGAGTA TGAAGTGGAT 
GAGTGCCAGA ACCAGCCATG CCAGAATGGG GGCACCTGTA TCGACCTTGT GAACCACTTC 
AAGTGCTCCT GCCCACCAGG CACTCGGGGC CTGCTTTGTG AAGAGAACGT TGACGACTGT 
GCCAGGGGTC CCCACTGCCT TAATGGTGGC CAGTGTGTAG ACAGGATTGG GGGCTACAGT 
TGTCGCTGCT TGCCAGGCTT TGCTGGAGAA CGCTGTGAAG GGGACATCAA CGAGTGCCTG 
TCCAACCCCT GCAGCTCTGA GGGCAGCCTG GACTGCATAC AGCTCACCAA CGACTACCTG 
TGCGTCTGCC GCAGCACCTT CACTGGTCGT CACTGTGAAA CGTTCGTCGA CGTGTGTCCC 
CAGATGCCTT GCCTGAACGG AGGCACTTGT GCTGTGGCCA GCAACATGCC CGATGGTTTC 
ATCTGTCGTT GTCCCCCGGG CTTCTCTGGG GCACGATGCC AGAGCAGCTG CGGACAGGTG 
AAGTGCAGGA GAGGGGAGCA ATGTGTGCAC ACGGCCTCGG GCCCCCGCTG CTTCTGCCCC 
AACCCCCAGG ACTGCGACTC GGGCTGCGCC AGTAGCCCCT GCCAGCACGG GGGGAGCTGC 
TACCCTCAGC GCCAGCCTCC TTACTACTCG TGCCAGTGTG CCCCGCCGTT CCGGGGCAGC 
CACTGTGAAC TCTACACCGC CCCCACCAGC ACCCCTCCCG CCACCTGCCT GAGCCAGTAT 
TGTGCTGACA AAGCTCGGGA CGGCATCTGT GATGACGTGT GCAACAGCCA TGCCTGCCAG 
TGGGATGGGG GGGACTGTTC CCTCACCATG GAGGACCCCT GGGCCAATTG CTCCTCCCCG 
CTGCCCTGCT GGGAGTACAT CAACAACCAG TGCGACGAGC TGTGCAACAC GGCCGAGTGC 
CTGTTTGACA ACTTTGAATG CCAGAAGAGT AGCAAGACAT GCAAATATGA CAAATACTGT 
GCAGACCACT ACGAAGACAA CCATTGTGAC CAGGGTTGCA ACAGTGAGGA GTGTGGCTGG 
GATGGGCTGG ACTGTGCTGC TGACCGGCCA GAAAACCTGG CAGAGGGCAC CCTGGTCATC 
GTGGTGCTGA TGCCACCTGA ACAGCTGCTC CAGGATGCCC GCAGATTCTT GCGGGCACTG 
GGCACCCTGC TCCACACCAA CCTCCGCATC AAACGGTCTG CCCAGGGGGA ACTCATGGTC 
TACCCCTATT ATGGTGGGAA GTCAGCTGCC ATGAAGAAGC AGAGACTGAC ACGCAGGTCC 
CTTCCTGGAG ATGAAGAACA GGAAGTGGCT GGCTCTCAGG TATTTCTGGA GATTGACAAC 
CGCCAGTGTG TTCAAGACTC AGACCAGTGC TTTAAGAACA CAGACGCAGC CGCAGCTCTT 
CTGGCTTCTC ACGCCATCCA GGGGACCCTG TCGTACCCAC TTGTGTCTGT CGTCAGTGAA 
TCTCTGACCC CAAAACCCAC TCAGCTTCTC TATCTGCTTG CTGTTGCCGT TGTCATTATC 
TTGTTTATTA TCCTCCTGGG GGTTATCATG GCAAAACGAA AGCGCAAGCA TGGCTCCCTC 
TGGCTGCCTG AAGGTTTCAC CCTTCGCCGT GACTCCAGCA ACCACAAACG TCGTGAGCCA 
GTGGGCCAGG ATGCTGTGGG ACTCAAAAAT CTTTCAGTGC AAGTCTCAGA GGCGAACCTA 
ATTGGTTCTG GAACAAGTGA ACACTGGGTC GATGATGAAG GGCCGCAGCC TAAGAAAGCC 
AAGGCTGAAG ATGAGGCTTT GCTCTCAGAA GAAGATGACC CCATCGATCG GCGTCCATGG 
ACACAGCAGC ACCTGGAAGC TGCAGACATC CGTAGGACAC CATCGCTGGC TCTCACCCCT 
CCACAGGCAG AGCAGGAGGT GGACGTGCTG GATGTGAACG TCCGCGGTCC AGATGGGTGC 
ACCCCACTGA TGCTGGCTTC TCTCCGAGGA GGCAGCTCAG ACATGAGTGA TGAAGATGAA 
GATGCAGAGG ACTCTTCTGC CAACATCATC ACGGACCTAG TCTACCAGGG TGCCAGCCTC 
CAAGCACAGA CAGACCGGAC GGGTGAGATG GCCCTGCATC TCGCAGCCCG GTACTCAAGG 
GCAGATGCTG CCAAGCGTCT CCTGGATGCA GGTGCAGATG CCAATGCCCA GGACAACATG 
GGCCGCTGCC CTCTCCATGC TGCAGTGGCT GCTGATGCCC AAGGTGTCTT CCAGATACTG 
ATCCGCAACC GAGTAACTGA TCTAGACGCA AGGATGAATG ATGGCACTAC ACCCCTGATC 
CTGGCTGCCC GCCTGGCAGT GGAGGGAATG GTGGCAGAAC TGATCAACTG CCAAGCAGAT 
GTGAATGCAG TGGATGACCA TGGAAAATCT GCCCTTCACT GGGCAGCTGC TGTGAATAAT 
GTGGAGGCAA CCCTTTTGCT GTTGAAAAAT GGGGCCAACC GAGACATGCA GGACAACAAG 
GAGGAGACAC CCCTGTTTCT TGCTGCCCGG GAGGGGAGCT ATGAAGCAGC CAAGATCCTG 
TTAGACCATT TTGCCAATCG GGACATCACA GACCACATGG ATCGCCTTCC CCGGGACGTG 
GCCCGGGACC GCATGCACCA TGACATCGTG CGCCTGCTGG ACGAGTACAA CGTGACCCCC 
AGCCCTCCCG GCACAGTGCT GACTTCTGCG CTCTCACCCG TCATCTGTGG GCCCAACAGA 
CCTTTTCTCA GTTTGAAGCA TACCCCAATG GGCAAGAAGT CTAGACGGCC TAACACCAAG 
AGTACCGTGC CCACTAGCCT CCCTAACCTT GCCAAGGAGG CCAAGGATGC CAAGGGGAAC 
AGGAGGAAGA AGTCTCTGAG TGACAAGGGC CAGCTGTCCG AGAGCTCAGT GACTCTGTCC 
CCCGTCGATT CCCTAGAATC TCCGCACACA TATGTTTCTG ACACCACCTC CTCTCCAATG 
ATCACATCCC CCGGAATCTT ACAGGCCTCC CCCAACCCCA TGCTGGCCAC TGCCGCCCCC 
ACTGCCCCAG TCCATGCACA GCATGCACTG TCTTTCTCTA ACCTTCATGA GATGCAGCCC 
TTGGCTCATG GGGCCAACAC TGTGCTTCCC TCGGTGAGCC AGCTGCTGTC TCACCGCCAC 
ATTGTGCCCC CGGGCAGCAG CACCGCAGGG AGCTTGGGTA GGCTGCATTC AGTCACTGTC 
CCCGCCGACT GGATGAACCG CGTGGAGATG AATGAGAGCC AGTACAACGA GATGTTCGGT 
ATGGTCCTGG CTCCAGCTGA GGGCACCCAC CCTGGCATAG CTCCCCAGAG CAGGCCGCCC 
GAAGGGAAGC ATTTAACCAC CCCACGAGAG CCCTTGCCCC CCATTGTGAC TTTCCAGCTA 
ATCCCCAAAG GCAGCATTGC CCAACCAACT GGGGCTGCCC AGCCTCCGTC CAACTGCCCT 
CCAGCTGTTG CAGGCACCCT GCCCACCATG TATCAGATTC CAGAAATGGC TCGCTTGCCC 
AGTATGGCTT TTCCCACTGC CATGATGCCC CAGCAGGATG GGCAGGTTGC TCAGACCATT 
CTCCCAGCCT ATCACGCCTT CCCAGCCTCC GTGGGCAAGT ATCCCACACC CCCATCACAG 
CATAGCTATG CTTCCTCCAA CGCTGCTGAG AGAACGCCCA GTCACAGTGG TCACCTCCAG 
GGTGAGCATC CCTACCTGAC ACCGTCCCCA GAGTCTCCTG ACCAGTGGTC AAGTTCATCA 
CCCCACTCTG CTTCTGACTG GTCAGATGTG ACCACCAGCC CCACTCCTGG GGGTGCTGGA 
GGAGGTCAGC GGGGACCTGG GACACACATG TCTGAGCCAC CACGCAGCAA CATGCAGGTC 
TATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_002686114.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686160.2 Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

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CloneID	OBa17547
Clone ID Related Accession (Same CDS sequence)	XM_002686114.5 , XM_867242.8
Accession Version	XM_002686114.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686114.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACCCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TCCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686160.2
CDS	257..7672
Translation	MPALRPAPLWALLALWLCRAAPARALQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKPTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_002686114.5
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686114.5

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACCCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TCCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_867242.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_872335.5 Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

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CloneID	OBa17547
Clone ID Related Accession (Same CDS sequence)	XM_002686114.5 , XM_867242.8
Accession Version	XM_867242.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_867242.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACCCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TCCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_872335.5
CDS	257..7672
Translation	MPALRPAPLWALLALWLCRAAPARALQCRDGYEPCVNEGICVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCAPGFTGEDCQHSTTHPCFVSHPCLNGGTCHVLSRDTYECTCQVGFTGKLCQWTDACLSHPCANGSTCTTMANQFSCTCLAGFTGQKCETDVNECDIPGQCQHGGTCLNLPGSYQCQCPQGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGVTCERNIDDCPNHKCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGSDCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCKNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNEGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCVHGVCMDGVNRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINDVNGFRCICPEGPHHPSCYSQVNECLSNPCVHGNCTGGLTGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCVLPYTGKNCQTVLAPCSPNPCENAGVCKEAPNFESYSCLCAPGWQGQRCTIDIDECVSKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCPAGFDGVHCENNIDECTESSCFNGGTCIDGINSFSCLCPVGFTGSFCLHEINECNSHPCLNEGVCVDGLGTYRCICPLGYTGKNCQTLVNLCSRSPCKNKGTCIQDKAESRCLCPSGWAGAYCDVPSVSCEVAASHRGVPVDRLCQHSGVCISAGNSHHCQCPLGYTGSYCEDQLDECSSNPCQHGATCRDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENVDDCARGPHCLNGGQCVDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSTFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPRCFCPNPQDCDSGCASSPCQHGGSCYPQRQPPYYSCQCAPPFRGSHCELYTAPTSTPPATCLSQYCADKARDGICDDVCNSHACQWDGGDCSLTMEDPWANCSSPLPCWEYINNQCDELCNTAECLFDNFECQKSSKTCKYDKYCADHYEDNHCDQGCNSEECGWDGLDCAADRPENLAEGTLVIVVLMPPEQLLQDARRFLRALGTLLHTNLRIKRSAQGELMVYPYYGGKSAAMKKQRLTRRSLPGDEEQEVAGSQVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPKPTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKAKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDMSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRPFLSLKHTPMGKKSRRPNTKSTVPTSLPNLAKEAKDAKGNRRKKSLSDKGQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPTAPVHAQHALSFSNLHEMQPLAHGANTVLPSVSQLLSHRHIVPPGSSTAGSLGRLHSVTVPADWMNRVEMNESQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHLTTPREPLPPIVTFQLIPKGSIAQPTGAAQPPSNCPPAVAGTLPTMYQIPEMARLPSMAFPTAMMPQQDGQVAQTILPAYHAFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPRSNMQVYA

Target ORF information:

RefSeq Version	XM_867242.8
Organism	Bos taurus(cattle)
Definition	Bos taurus notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_867242.8

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
GCCCCGGCGC GTGCATTGCA ATGTCGGGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA 
TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA 
TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG 
GCCCAGGCTA TGTTGGGGAA AGCCACGTGC CGCTGTGCCC CGGGCTTCAC GGGCGAGGAC 
TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC 
TGCCACGTGC TTAGCCGGGA CACCTACGAG TGTACCTGCC AAGTCGGTTT TACAGGTAAA 
CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC 
ACTATGGCCA ACCAGTTCTC CTGCACATGC CTTGCAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC 
CTCCCAGGCT CCTATCAGTG CCAATGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT 
CCCTATGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAGACCGGT 
GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT 
ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT 
ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAGTGCCTGC TGCAGCCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG 
GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT 
GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACCTGCA TCGATCGTGT GGCCTCCTTC 
TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC 
AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC 
ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC 
CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CACTCAGACC CCTGCAAGAA TGATGCCACC TGTTTGGATA AGATTGGAGG CTTCACGTGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT 
CCGCCTGGAT TCACGGGACC AGTTTGCCAG ATTGATATTG ATGACTGTTC TAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA 
GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC 
CACGGCCAGT GTCAGGATGG CATTGATTCC TACACCTGCA TTTGCAATCC GGGCTACATG 
GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT 
CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT 
AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG 
GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACGTG CATCAATGAT 
GTGAATGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG 
GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CATGGAAACT GTACTGGGGG CCTCACTGGA 
TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT 
TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG 
CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT 
CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC 
TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC 
GGAGGAACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT 
GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACTGGTTCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT 
GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT 
CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG 
GATAAAGCAG AGTCGCGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT 
CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC 
CAGCACTCAG GCGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC 
TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC 
GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG 
GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC 
ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG 
CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG 
TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC 
TGTGAAGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGCATACAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC 
TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT 
GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCA 
CGATGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAATG TGTGCACACG 
GCCTCGGGCC CCCGCTGCTT CTGCCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT 
AGCCCCTGCC AGCACGGGGG GAGCTGCTAC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC 
CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC 
CCTCCCGCCA CCTGCCTGAG CCAGTATTGT GCTGACAAAG CTCGGGACGG CATCTGTGAT 
GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG 
GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC 
GACGAGCTGT GCAACACGGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC 
AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG 
GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA 
AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA GCTGCTCCAG 
GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA 
CGGTCTGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG 
AAGAAGCAGA GACTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC 
TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT 
AAGAACACAG ACGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG 
TACCCACTTG TGTCTGTCGT CAGTGAATCT CTGACCCCAA AACCCACTCA GCTTCTCTAT 
CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TCCTGGGGGT TATCATGGCA 
AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC 
TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTT 
TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA 
GATGACCCCA TCGATCGGCG TCCATGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT 
GTGAACGTCC GCGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC 
AGCTCAGACA TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCCAA CATCATCACG 
GACCTAGTCT ACCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC 
CTGCATCTCG CAGCCCGGTA CTCAAGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCTGCT 
GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG 
ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC 
CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC 
CACATGGATC GCCTTCCCCG GGACGTGGCC CGGGACCGCA TGCACCATGA CATCGTGCGC 
CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC TTCTGCGCTC 
TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC 
AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG 
CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT 
GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC 
AACCCCATGC TGGCCACTGC CGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT 
TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCG 
GTGAGCCAGC TGCTGTCTCA CCGCCACATT GTGCCCCCGG GCAGCAGCAC CGCAGGGAGC 
TTGGGTAGGC TGCATTCAGT CACTGTCCCC GCCGACTGGA TGAACCGCGT GGAGATGAAT 
GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT 
GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC ACGAGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG 
GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCTGTTGCAG GCACCCTGCC CACCATGTAT 
CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG 
CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCCGTG 
GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCTT CCTCCAACGC TGCTGAGAGA 
ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC GTCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCCA CTCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NOTCH2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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