COL27A1 cDNA ORF clone, Bos taurus(cattle)

The following COL27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237053 XM_024995679.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237054 XM_024995680.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237055 XM_024995681.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237056 XM_024995682.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237057 XM_024995683.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237058 XM_024995684.1
Latest version!
Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa36307 NM_001206680.1
Latest version!
Bos taurus collagen, type XXVII, alpha 1 (COL27A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237053
    Clone ID Related Accession (Same CDS sequence) XM_024995679.1
    Accession Version XM_024995679.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGGTCCT CCCGGTCCCA GAGGGCAGCC CGGCCCACCG GGTCCTCCAG GGAGCACTAT
    CCACTTCGTA AGTTGGAGAA CTTCCTCTGC GGTCTACTTG GACAACAAGA TGACCTTGGG
    GCAGCTTTCC AGACGTGGAT GGATACAAGT GGAGCACTGA GAGCAGAGGG TTACAGCCAT
    CCGGACCGGC TGGTGCTGGA CCAGGGAGGG GAGATCTTTA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACGCCCCTG GGCACCAAAG AGAACCCCGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAAGATG GTGGATGGTA CCTACTGGGT GGATCCTAAC
    CTTGGCTGCT CGTCCGACAC CATCGAAGTC TCCTGCAACT TCACACATGG TGGACAGACG
    TGTCTCAAGC CCATCACGGC CTCCAAGGTG GAGTTTGCCA TCAGCCGGGT CCAGATGAAT
    TTCCTGCACC TGCTAAGCTC GGAGGTGACA CAGCAGATCA CCATCCACTG CCTTAACATG
    ACCGTGTGGC AGGAGGGCAC CGGGCAGACC CCAGCTAAGC AGGCTGTGCG CTTCCGGGCC
    TGGAACGGGC AGATCTTTGA GGCCGGGGGT CAGTTCAGTC CAGAAGTGTC CATGGATGGC
    TGCAAGATCC AGGATGGCCG CTGGCATCAG ACGCTCTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATTGTCGG CGTGGACAAC CTCCCTCCCG CCTCATCAGG GAAGCAGTAC
    CGCCTCGAAG TTGGACCTGC ATGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851447.1
    CDS486..6038
    Translation

    Target ORF information:

    RefSeq Version XM_024995679.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995679.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGGTCCT CCCGGTCCCA GAGGGCAGCC CGGCCCACCG GGTCCTCCAG GGAGCACTAT
    CCACTTCGTA AGTTGGAGAA CTTCCTCTGC GGTCTACTTG GACAACAAGA TGACCTTGGG
    GCAGCTTTCC AGACGTGGAT GGATACAAGT GGAGCACTGA GAGCAGAGGG TTACAGCCAT
    CCGGACCGGC TGGTGCTGGA CCAGGGAGGG GAGATCTTTA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACGCCCCTG GGCACCAAAG AGAACCCCGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAAGATG GTGGATGGTA CCTACTGGGT GGATCCTAAC
    CTTGGCTGCT CGTCCGACAC CATCGAAGTC TCCTGCAACT TCACACATGG TGGACAGACG
    TGTCTCAAGC CCATCACGGC CTCCAAGGTG GAGTTTGCCA TCAGCCGGGT CCAGATGAAT
    TTCCTGCACC TGCTAAGCTC GGAGGTGACA CAGCAGATCA CCATCCACTG CCTTAACATG
    ACCGTGTGGC AGGAGGGCAC CGGGCAGACC CCAGCTAAGC AGGCTGTGCG CTTCCGGGCC
    TGGAACGGGC AGATCTTTGA GGCCGGGGGT CAGTTCAGTC CAGAAGTGTC CATGGATGGC
    TGCAAGATCC AGGATGGCCG CTGGCATCAG ACGCTCTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATTGTCGG CGTGGACAAC CTCCCTCCCG CCTCATCAGG GAAGCAGTAC
    CGCCTCGAAG TTGGACCTGC ATGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237054
    Clone ID Related Accession (Same CDS sequence) XM_024995680.1
    Accession Version XM_024995680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGTTAG GCGCCCGAGG AAGGACCGAC CGTCCAGCCT CGCACCTAGG GATCCAGGGG 
    TTTCTCTTCA CCTGGATCTT AGTCTCGCTC ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGACGTCCT CCAGCAGCTG GGCCTCAGCT GGACGAAGGC GGTGGGCGGC
    CGGAGCCCCC CGCCCCCAGG GGTCATCCCG TTCCAGACGG GCTTCATCTT CACGCAGCGG
    GCCCGGCTCC AGGCTCCCAC GGCTGCCGTC CTCCCCGCCT CCCTGGGCAC GGAGCTGGCG
    CTGGTGCTCA GCCTCTGCTC CCACCGGGTG AACCATGCCT TCCTCTTCGC CGTCCGCAGC
    CGGAAGCGCA AGCTGCAGCT GGGCCTGCAG TTCCTCCCCG GCAAGACTGT GGTCCACCTG
    GGGCCCCGGC GCTCCGTGGC CTTCGACCTC GACGTCCACG ACGGGCGCTG GCACCACCTG
    GCCCTGGAGC TCCGCGGCCG CACGGTCACC CTGGTGACGG CCTGCGGGCA GCACCGGGTG
    CCCGTCTCGC TGCCTTTCCA CAGGGACCCA GCGCTCGACC CTGACGGCTT GTTCCTCTTT
    GGGAAGATGA GCCCGCACGC GGTCCAGTTC GAGGGGGCCC TGTGCCAGTT CAGTATCTAC
    CCCGTGGCGC AGGTCGCTCA CAATTACTGT ACCCACCTGA GAAAGCAGTG TGGACAGGCT
    GACACCTACC GGCCCCAGCT GGGACCCTTC TTCCCCCGAG ACTCTGGCAC ACTCCTCACC
    TTCCAGACCG ACCTCGCCCG GTTGGGCCTG GAGAATCTGA CCACTGCCAC GCCAGCCCTG
    GGGTCACGGC CGGCAGGCAG GGGGCCTGGG GTGACTGTGG TGCCTGCTGT GCCCGTCAAG
    CCCATGAGGA CTAGCACCAC ACGCCCGCCC CAGCCCATCA CATCCCATCC AGCCTGGACC
    CCTCTGTCCC CTGCCAAGCT ATCAGTCAGT GAAGGGCCTC CTCCCGTGTC CCCGGTCTCT
    CCTGCCAGCT CCCCCAGACC TGTCCAGCCA TTGCAGAAGA ACACAGCCAC TAAGACCTCC
    AAAAGTCACC CCGCCACACC TTCAGCCCTT TCTCCTTCAA TTGCCCCAGT CAGAAGCCCC
    CGCCCCACCC AGAAGACAGC TCTGCCTGCC TTCACAAAGC CAGCCCCACC CACCAAGAAG
    CCGGTGCTAC CTACTTCCCA GCCCATTCCT GCCAAAGTCT CCCGTCCCAC AGTGAAGCCG
    ATCCAGAGGA ACCCCGGCAT GCCCAGACTC CCGCCACCCA GTGCCCGGCC CCTACCTCCA
    GCTGCTGCCT CCTCTAAAAA GCCTTTGCCC CCAGTAGTCC AGGCTGAGGC CAAGATGAGT
    AGTCGTGCCA GTGAGCCGGC TCCTGCCCAT ACCAGCACAC ACAGACCTCC CCCACCCACA
    GTCCCGCCCC CATCACCTGT CCCCAGTCCT AGCCCTCCCA GGACCGCTCG GCCACCAGCC
    ACAGCGATGC CTCCCACTCT CGCTCCTGGT TCAGCCTCCA CTGGAAGCAA GAAACCCACT
    GGATCAGAAG CCACAAAGAA AGCCAGACCC CGGAAGCCCG TCCCACTCAG ATCTGGGAAG
    GCAGCCCGGG ATGTCCCGCT AAACGATCCA ACAAGGGGGT CCAGCCCCAG ACAGCCCCGG
    ACCAGGCAGC AGACCACCCC GGCCCCAGCG CTGGCCCCGG CGCGATTCCT GGCCTCCAGC
    CCCCAGCCCA TGAGTACTGG CTATTCGTTC TTCCACCTGG TGGGACCCAC ACCTTTCCTG
    CTGCTGATGG GACCTCCAGG ACCCAAGGGA GACTGTGGTT TGCCGGGTCC CCCTGGACTG
    CCTGGGCTTC CTGGACCCCC TGGCGCACGG GGCCCTCGGG GTCCTCCTGG GCCTTATGGA
    AATCCAGGCC TTCCTGGCCC CCCTGGAGCC AAAGGACAGA AAGGGGACCC CGGGCTCTCA
    CCAGGAAAGG CCCACGATGG GGCAAAGGGC GACATGGGCT TGCCCGGGCT CACTGGGAAT
    CCAGGACCCC TTGGACGAAA GGGACAGAAG GGTTATCCTG GACCAACGGG GCACCCCGGA
    GAACAGGGGC AGCCAGGACC CGAGGGTAGC CCAGGGGCCA AAGGTTACCC TGGCAGGCAG
    GGGCTGCCTG GACCTGTAGG AGACCCTGGG CCCAAAGGTA GCCGGGGCTA CATTGGACTC
    CCGGGGCTCT TCGGCCTGCC AGGGTCTGAT GGAGAGCGAG GTCTGCCTGG CGTTCCTGGC
    AAGAGGGGCA AGATGGGTAG GCCGGGGTTT CCTGGAGACT TTGGGGAAAG AGGCCCTCCA
    GGACTGGATG GAAACCCTGG AGAACTGGGC CTGCCAGGGC CCCCAGGAGT CCCCGGCCTC
    ATTGGCGACA TGGGAACATT GGGTCCGATT GGCTACCCAG GACCCAAGGG CATGAAGGGA
    CTGATGGGAA ACGTGGGGGA ACCCGGACTG AAAGGTGATA AGGGTGAACA AGGGGTTCCA
    GGTGTGTCAG GAGACGCCGG ATTCCAAGGA GACAAGGGGA GCCAGGGCTT GCAAGGGTTC
    CCTGGCGCAC GGGGGAAGCC AGGGCCAGTG GGCAAAGTTG GAGACAAAGG ATCCATTGGG
    TTTCCTGGGC CCCCGGGACC TGAGGGATTC CCTGGAGACA TTGGTCCCCC TGGGGACAAC
    GGCCCAGAAG GCGTGAAGGG TAAGCCTGGA GCTCGAGGCC TGCCAGGACC CCGTGGGCAG
    CTGGGGCCCG AGGGAGATGA GGGGCCCATG GGGCCACCAG GGGTCCCTGG CTTGGAGGGT
    CAACCCGGCA GGAAGGGGTT TCCTGGAAGA CCTGGCCCGG ATGGCTTGAA GGGCGAGCCA
    GGCGATGCTG GACGGCCGGG GCCTGTGGGT GAGCAGGGAC TTCTGGGATT CGTTGGTCTG
    GTCGGAGAGC CAGGAATCAT GGGAGAAAAG GGTGACCGGG GCATGATGGG ACCCCCAGGC
    GCGCCCGGAC CCAAGGGGTC GATGGGCCAT CCTGGAATCC CAGGTGTGGT GGGAAACCCG
    GGAGAGCCTG GACCTCCGGG TCCTCCAGGA TCTCGAGGCC CACCGGGCAT GAGGGGAGCA
    AAGGGACGCC GGGGACCCCG AGGACCAGAC GGACCAGCTG GGGAGCAGGG GTCCAAGGGC
    CTGAAGGGCC CGCCAGGACC ACAGGGCAGA CTGGGCCAGC CCGGGCAGCA GGGCGTGGCT
    GGCGAGCGAG GGCACTTGGG CACACGAGGC TTTCCTGGCC TCCGGGGTCC TTCCGGTCCC
    CCAGGCGCCA AGGGCCTCCC AGGAGAACCG GGCCCTCAGG GACCCCAGGG GCCAATTGGC
    CCTCCCGGAG AGATGGGACC CAAGGGACCA CCAGGTGCAT TGGGAGAACT GGGCCTTCCT
    GGGGAAGCTG GCATGAAGGG TGACCTTGGG CCTCTGGGCA TCCCTGGGGA GCAGGGCCTC
    ATTGGGCAGC GGGGAGAGCC AGGCCTCGAG GGTGACAGTG GCCCTGCAGG GCCTGACGGG
    CTGAAGGGGG ACAGGGGTGA CCCCGGGCCT GACGGTGAAC GTGGCGAGAA GGGCCAAGAG
    GGACTGAAGG GCGAGGACGG GCCCCCCGGC CCCCCTGGCA TCACTGGCAT CCGGGGTCCT
    GAAGGAAAAC CAGGGAAGCA AGGCGAGAAG GGCCGGACGG GAGCCAAGGG TGCCAAGGGC
    TACCAAGGAC AGCTGGGTGA GATGGGCGTC CCTGGAGACC CCGGACCCCC TGGCACCCCA
    GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA CCAACGGGTG CTCCAGGACG GATGGGGGCC
    CAAGGAGAGC CGGGACTGGC TGGTTACGAC GGACACAAAG GCATCATGGG ACCCCTCGGA
    CCTCCTGGAC CAAAGGGTGA AAAGGGGGAG CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC
    CCTGGACCAC CTGGAGATCG GGGCCCTGTG GGTGATCGAG GAGACCGTGG CGAACCAGGG
    GACCCTGGAT ACCCTGGACA GGAGGGTGTA CAAGGCCTCC GTGGAAATCC AGGCCAGCAG
    GGCCAACCCG GGCATCCGGG ACCCCGGGGA CGGCCGGGCC CCAAAGGATC GAAAGGGGAA
    GAGGGGCCAA AGGGAAAGCA AGGCAAGGCT GGGGCGCCAG GACGGAGGGG GATCCAGGGC
    CTGCAGGGGC TCCCAGGGCC TCGGGGCGTG GTGGGGAGAC AGGGCCCCGA GGGTGTTGCT
    GGACCAGATG GGCTTCCTGG CCGCGATGGT CAAGCAGGAC AGCAGGGGGA GCAAGGAGAT
    GACGGGGACC CCGGGCCCAT GGGCCCTGCT GGGAAGAGAG GAGATCCAGG TGTGGCTGGC
    TTGCCTGGAG CACAGGGGCC CCCAGGATTC AAGGGTGAAA GTGGGTTGCC TGGGCAGATG
    GGACCCCCTG GCAAGCGAGG GACAGAAGGC GGCATGGGGC TTCCTGGGAA CCAGGGGGAG
    CCTGGATCCA AAGGCCAGCC GGGTGACTCT GGTGAGATGG GATTCCCAGG AATGGCTGGC
    CTCTTTGGAC CCAAGGGCCC CCAAGGAGAC GTCGGTTTCA AAGGCATCCA GGGCCCTCGG
    GGGCCTCCTG GCTTGATGGG AAAGGAAGGT ATCATCGGGC CCCTGGGAAT CCTGGGACCT
    TCGGGACGCC AGGGTCCAAA GGGCGACAAA GGCAGCCGCG GGGACTTGGG ATTGCAAGGG
    TCCTCCCGGT CCCAGAGGGC AGCCCGGCCC ACCGGGTCCT CCAGGGAGCA CTATCCACTT
    CGTAAGTTGG AGAACTTCCT CTGCGGTCTA CTTGGACAAC AAGATGACCT TGGGGCAGCT
    TTCCAGACGT GGATGGATAC AAGTGGAGCA CTGAGAGCAG AGGGTTACAG CCATCCGGAC
    CGGCTGGTGC TGGACCAGGG AGGGGAGATC TTTAAAACCT TACACTACCT CAGCAACCTC
    ATCCAGAGCA TTAAGACGCC CCTGGGCACC AAAGAGAACC CCGCCCGGGT CTGCCGGGAC
    CTCATGGACT GTGAGCAGAA GATGGTGGAT GGTACCTACT GGGTGGATCC TAACCTTGGC
    TGCTCGTCCG ACACCATCGA AGTCTCCTGC AACTTCACAC ATGGTGGACA GACGTGTCTC
    AAGCCCATCA CGGCCTCCAA GGTGGAGTTT GCCATCAGCC GGGTCCAGAT GAATTTCCTG
    CACCTGCTAA GCTCGGAGGT GACACAGCAG ATCACCATCC ACTGCCTTAA CATGACCGTG
    TGGCAGGAGG GCACCGGGCA GACCCCAGCT AAGCAGGCTG TGCGCTTCCG GGCCTGGAAC
    GGGCAGATCT TTGAGGCCGG GGGTCAGTTC AGTCCAGAAG TGTCCATGGA TGGCTGCAAG
    ATCCAGGATG GCCGCTGGCA TCAGACGCTC TTCACCTTCC GGACCCAGGA CCCCCAGCAG
    CTGCCCATTG TCGGCGTGGA CAACCTCCCT CCCGCCTCAT CAGGGAAGCA GTACCGCCTC
    GAAGTTGGAC CTGCATGCTT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851448.1
    CDS52..5598
    Translation

    Target ORF information:

    RefSeq Version XM_024995680.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGTTAG GCGCCCGAGG AAGGACCGAC CGTCCAGCCT CGCACCTAGG GATCCAGGGG 
    TTTCTCTTCA CCTGGATCTT AGTCTCGCTC ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGACGTCCT CCAGCAGCTG GGCCTCAGCT GGACGAAGGC GGTGGGCGGC
    CGGAGCCCCC CGCCCCCAGG GGTCATCCCG TTCCAGACGG GCTTCATCTT CACGCAGCGG
    GCCCGGCTCC AGGCTCCCAC GGCTGCCGTC CTCCCCGCCT CCCTGGGCAC GGAGCTGGCG
    CTGGTGCTCA GCCTCTGCTC CCACCGGGTG AACCATGCCT TCCTCTTCGC CGTCCGCAGC
    CGGAAGCGCA AGCTGCAGCT GGGCCTGCAG TTCCTCCCCG GCAAGACTGT GGTCCACCTG
    GGGCCCCGGC GCTCCGTGGC CTTCGACCTC GACGTCCACG ACGGGCGCTG GCACCACCTG
    GCCCTGGAGC TCCGCGGCCG CACGGTCACC CTGGTGACGG CCTGCGGGCA GCACCGGGTG
    CCCGTCTCGC TGCCTTTCCA CAGGGACCCA GCGCTCGACC CTGACGGCTT GTTCCTCTTT
    GGGAAGATGA GCCCGCACGC GGTCCAGTTC GAGGGGGCCC TGTGCCAGTT CAGTATCTAC
    CCCGTGGCGC AGGTCGCTCA CAATTACTGT ACCCACCTGA GAAAGCAGTG TGGACAGGCT
    GACACCTACC GGCCCCAGCT GGGACCCTTC TTCCCCCGAG ACTCTGGCAC ACTCCTCACC
    TTCCAGACCG ACCTCGCCCG GTTGGGCCTG GAGAATCTGA CCACTGCCAC GCCAGCCCTG
    GGGTCACGGC CGGCAGGCAG GGGGCCTGGG GTGACTGTGG TGCCTGCTGT GCCCGTCAAG
    CCCATGAGGA CTAGCACCAC ACGCCCGCCC CAGCCCATCA CATCCCATCC AGCCTGGACC
    CCTCTGTCCC CTGCCAAGCT ATCAGTCAGT GAAGGGCCTC CTCCCGTGTC CCCGGTCTCT
    CCTGCCAGCT CCCCCAGACC TGTCCAGCCA TTGCAGAAGA ACACAGCCAC TAAGACCTCC
    AAAAGTCACC CCGCCACACC TTCAGCCCTT TCTCCTTCAA TTGCCCCAGT CAGAAGCCCC
    CGCCCCACCC AGAAGACAGC TCTGCCTGCC TTCACAAAGC CAGCCCCACC CACCAAGAAG
    CCGGTGCTAC CTACTTCCCA GCCCATTCCT GCCAAAGTCT CCCGTCCCAC AGTGAAGCCG
    ATCCAGAGGA ACCCCGGCAT GCCCAGACTC CCGCCACCCA GTGCCCGGCC CCTACCTCCA
    GCTGCTGCCT CCTCTAAAAA GCCTTTGCCC CCAGTAGTCC AGGCTGAGGC CAAGATGAGT
    AGTCGTGCCA GTGAGCCGGC TCCTGCCCAT ACCAGCACAC ACAGACCTCC CCCACCCACA
    GTCCCGCCCC CATCACCTGT CCCCAGTCCT AGCCCTCCCA GGACCGCTCG GCCACCAGCC
    ACAGCGATGC CTCCCACTCT CGCTCCTGGT TCAGCCTCCA CTGGAAGCAA GAAACCCACT
    GGATCAGAAG CCACAAAGAA AGCCAGACCC CGGAAGCCCG TCCCACTCAG ATCTGGGAAG
    GCAGCCCGGG ATGTCCCGCT AAACGATCCA ACAAGGGGGT CCAGCCCCAG ACAGCCCCGG
    ACCAGGCAGC AGACCACCCC GGCCCCAGCG CTGGCCCCGG CGCGATTCCT GGCCTCCAGC
    CCCCAGCCCA TGAGTACTGG CTATTCGTTC TTCCACCTGG TGGGACCCAC ACCTTTCCTG
    CTGCTGATGG GACCTCCAGG ACCCAAGGGA GACTGTGGTT TGCCGGGTCC CCCTGGACTG
    CCTGGGCTTC CTGGACCCCC TGGCGCACGG GGCCCTCGGG GTCCTCCTGG GCCTTATGGA
    AATCCAGGCC TTCCTGGCCC CCCTGGAGCC AAAGGACAGA AAGGGGACCC CGGGCTCTCA
    CCAGGAAAGG CCCACGATGG GGCAAAGGGC GACATGGGCT TGCCCGGGCT CACTGGGAAT
    CCAGGACCCC TTGGACGAAA GGGACAGAAG GGTTATCCTG GACCAACGGG GCACCCCGGA
    GAACAGGGGC AGCCAGGACC CGAGGGTAGC CCAGGGGCCA AAGGTTACCC TGGCAGGCAG
    GGGCTGCCTG GACCTGTAGG AGACCCTGGG CCCAAAGGTA GCCGGGGCTA CATTGGACTC
    CCGGGGCTCT TCGGCCTGCC AGGGTCTGAT GGAGAGCGAG GTCTGCCTGG CGTTCCTGGC
    AAGAGGGGCA AGATGGGTAG GCCGGGGTTT CCTGGAGACT TTGGGGAAAG AGGCCCTCCA
    GGACTGGATG GAAACCCTGG AGAACTGGGC CTGCCAGGGC CCCCAGGAGT CCCCGGCCTC
    ATTGGCGACA TGGGAACATT GGGTCCGATT GGCTACCCAG GACCCAAGGG CATGAAGGGA
    CTGATGGGAA ACGTGGGGGA ACCCGGACTG AAAGGTGATA AGGGTGAACA AGGGGTTCCA
    GGTGTGTCAG GAGACGCCGG ATTCCAAGGA GACAAGGGGA GCCAGGGCTT GCAAGGGTTC
    CCTGGCGCAC GGGGGAAGCC AGGGCCAGTG GGCAAAGTTG GAGACAAAGG ATCCATTGGG
    TTTCCTGGGC CCCCGGGACC TGAGGGATTC CCTGGAGACA TTGGTCCCCC TGGGGACAAC
    GGCCCAGAAG GCGTGAAGGG TAAGCCTGGA GCTCGAGGCC TGCCAGGACC CCGTGGGCAG
    CTGGGGCCCG AGGGAGATGA GGGGCCCATG GGGCCACCAG GGGTCCCTGG CTTGGAGGGT
    CAACCCGGCA GGAAGGGGTT TCCTGGAAGA CCTGGCCCGG ATGGCTTGAA GGGCGAGCCA
    GGCGATGCTG GACGGCCGGG GCCTGTGGGT GAGCAGGGAC TTCTGGGATT CGTTGGTCTG
    GTCGGAGAGC CAGGAATCAT GGGAGAAAAG GGTGACCGGG GCATGATGGG ACCCCCAGGC
    GCGCCCGGAC CCAAGGGGTC GATGGGCCAT CCTGGAATCC CAGGTGTGGT GGGAAACCCG
    GGAGAGCCTG GACCTCCGGG TCCTCCAGGA TCTCGAGGCC CACCGGGCAT GAGGGGAGCA
    AAGGGACGCC GGGGACCCCG AGGACCAGAC GGACCAGCTG GGGAGCAGGG GTCCAAGGGC
    CTGAAGGGCC CGCCAGGACC ACAGGGCAGA CTGGGCCAGC CCGGGCAGCA GGGCGTGGCT
    GGCGAGCGAG GGCACTTGGG CACACGAGGC TTTCCTGGCC TCCGGGGTCC TTCCGGTCCC
    CCAGGCGCCA AGGGCCTCCC AGGAGAACCG GGCCCTCAGG GACCCCAGGG GCCAATTGGC
    CCTCCCGGAG AGATGGGACC CAAGGGACCA CCAGGTGCAT TGGGAGAACT GGGCCTTCCT
    GGGGAAGCTG GCATGAAGGG TGACCTTGGG CCTCTGGGCA TCCCTGGGGA GCAGGGCCTC
    ATTGGGCAGC GGGGAGAGCC AGGCCTCGAG GGTGACAGTG GCCCTGCAGG GCCTGACGGG
    CTGAAGGGGG ACAGGGGTGA CCCCGGGCCT GACGGTGAAC GTGGCGAGAA GGGCCAAGAG
    GGACTGAAGG GCGAGGACGG GCCCCCCGGC CCCCCTGGCA TCACTGGCAT CCGGGGTCCT
    GAAGGAAAAC CAGGGAAGCA AGGCGAGAAG GGCCGGACGG GAGCCAAGGG TGCCAAGGGC
    TACCAAGGAC AGCTGGGTGA GATGGGCGTC CCTGGAGACC CCGGACCCCC TGGCACCCCA
    GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA CCAACGGGTG CTCCAGGACG GATGGGGGCC
    CAAGGAGAGC CGGGACTGGC TGGTTACGAC GGACACAAAG GCATCATGGG ACCCCTCGGA
    CCTCCTGGAC CAAAGGGTGA AAAGGGGGAG CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC
    CCTGGACCAC CTGGAGATCG GGGCCCTGTG GGTGATCGAG GAGACCGTGG CGAACCAGGG
    GACCCTGGAT ACCCTGGACA GGAGGGTGTA CAAGGCCTCC GTGGAAATCC AGGCCAGCAG
    GGCCAACCCG GGCATCCGGG ACCCCGGGGA CGGCCGGGCC CCAAAGGATC GAAAGGGGAA
    GAGGGGCCAA AGGGAAAGCA AGGCAAGGCT GGGGCGCCAG GACGGAGGGG GATCCAGGGC
    CTGCAGGGGC TCCCAGGGCC TCGGGGCGTG GTGGGGAGAC AGGGCCCCGA GGGTGTTGCT
    GGACCAGATG GGCTTCCTGG CCGCGATGGT CAAGCAGGAC AGCAGGGGGA GCAAGGAGAT
    GACGGGGACC CCGGGCCCAT GGGCCCTGCT GGGAAGAGAG GAGATCCAGG TGTGGCTGGC
    TTGCCTGGAG CACAGGGGCC CCCAGGATTC AAGGGTGAAA GTGGGTTGCC TGGGCAGATG
    GGACCCCCTG GCAAGCGAGG GACAGAAGGC GGCATGGGGC TTCCTGGGAA CCAGGGGGAG
    CCTGGATCCA AAGGCCAGCC GGGTGACTCT GGTGAGATGG GATTCCCAGG AATGGCTGGC
    CTCTTTGGAC CCAAGGGCCC CCAAGGAGAC GTCGGTTTCA AAGGCATCCA GGGCCCTCGG
    GGGCCTCCTG GCTTGATGGG AAAGGAAGGT ATCATCGGGC CCCTGGGAAT CCTGGGACCT
    TCGGGACGCC AGGGTCCAAA GGGCGACAAA GGCAGCCGCG GGGACTTGGG ATTGCAAGGG
    TCCTCCCGGT CCCAGAGGGC AGCCCGGCCC ACCGGGTCCT CCAGGGAGCA CTATCCACTT
    CGTAAGTTGG AGAACTTCCT CTGCGGTCTA CTTGGACAAC AAGATGACCT TGGGGCAGCT
    TTCCAGACGT GGATGGATAC AAGTGGAGCA CTGAGAGCAG AGGGTTACAG CCATCCGGAC
    CGGCTGGTGC TGGACCAGGG AGGGGAGATC TTTAAAACCT TACACTACCT CAGCAACCTC
    ATCCAGAGCA TTAAGACGCC CCTGGGCACC AAAGAGAACC CCGCCCGGGT CTGCCGGGAC
    CTCATGGACT GTGAGCAGAA GATGGTGGAT GGTACCTACT GGGTGGATCC TAACCTTGGC
    TGCTCGTCCG ACACCATCGA AGTCTCCTGC AACTTCACAC ATGGTGGACA GACGTGTCTC
    AAGCCCATCA CGGCCTCCAA GGTGGAGTTT GCCATCAGCC GGGTCCAGAT GAATTTCCTG
    CACCTGCTAA GCTCGGAGGT GACACAGCAG ATCACCATCC ACTGCCTTAA CATGACCGTG
    TGGCAGGAGG GCACCGGGCA GACCCCAGCT AAGCAGGCTG TGCGCTTCCG GGCCTGGAAC
    GGGCAGATCT TTGAGGCCGG GGGTCAGTTC AGTCCAGAAG TGTCCATGGA TGGCTGCAAG
    ATCCAGGATG GCCGCTGGCA TCAGACGCTC TTCACCTTCC GGACCCAGGA CCCCCAGCAG
    CTGCCCATTG TCGGCGTGGA CAACCTCCCT CCCGCCTCAT CAGGGAAGCA GTACCGCCTC
    GAAGTTGGAC CTGCATGCTT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237055
    Clone ID Related Accession (Same CDS sequence) XM_024995681.1
    Accession Version XM_024995681.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGCC ATCCTGGAAT CCCAGGTGTG
    GTGGGAAACC CGGGAGAGCC TGGACCTCCG GGTCCTCCAG GATCTCGAGG CCCACCGGGC
    ATGAGGGGAG CAAAGGGACG CCGGGGACCC CGAGGACCAG ACGGACCAGC TGGGGAGCAG
    GGGTCCAAGG GCCTGAAGGG CCCGCCAGGA CCACAGGGCA GACTGGGCCA GCCCGGGCAG
    CAGGGCGTGG CTGGCGAGCG AGGGCACTTG GGCACACGAG GCTTTCCTGG CCTCCGGGGT
    CCTTCCGGTC CCCCAGGCGC CAAGGGCCTC CCAGGAGAAC CGGGCCCTCA GGGACCCCAG
    GGGCCAATTG GCCCTCCCGG AGAGATGGGA CCCAAGGGAC CACCAGGTGC ATTGGGAGAA
    CTGGGCCTTC CTGGGGAAGC TGGCATGAAG GGTGACCTTG GGCCTCTGGG CATCCCTGGG
    GAGCAGGGCC TCATTGGGCA GCGGGGAGAG CCAGGCCTCG AGGGTGACAG TGGCCCTGCA
    GGGCCTGACG GGCTGAAGGG GGACAGGGGT GACCCCGGGC CTGACGGTGA ACGTGGCGAG
    AAGGGCCAAG AGGGACTGAA GGGCGAGGAC GGGCCCCCCG GCCCCCCTGG CATCACTGGC
    ATCCGGGGTC CTGAAGGAAA ACCAGGGAAG CAAGGCGAGA AGGGCCGGAC GGGAGCCAAG
    GGTGCCAAGG GCTACCAAGG ACAGCTGGGT GAGATGGGCG TCCCTGGAGA CCCCGGACCC
    CCTGGCACCC CAGGCCCTAA AGGGTCCCGG GGCAGCCTGG GACCAACGGG TGCTCCAGGA
    CGGATGGGGG CCCAAGGAGA GCCGGGACTG GCTGGTTACG ACGGACACAA AGGCATCATG
    GGACCCCTCG GACCTCCTGG ACCAAAGGGT GAAAAGGGGG AGCAGGGCGA GGACGGCAAG
    GCTGAGGGGC CCCCTGGACC ACCTGGAGAT CGGGGCCCTG TGGGTGATCG AGGAGACCGT
    GGCGAACCAG GGGACCCTGG ATACCCTGGA CAGGAGGGTG TACAAGGCCT CCGTGGAAAT
    CCAGGCCAGC AGGGCCAACC CGGGCATCCG GGACCCCGGG GACGGCCGGG CCCCAAAGGA
    TCGAAAGGGG AAGAGGGGCC AAAGGGAAAG CAAGGCAAGG CTGGGGCGCC AGGACGGAGG
    GGGATCCAGG GCCTGCAGGG GCTCCCAGGG CCTCGGGGCG TGGTGGGGAG ACAGGGCCCC
    GAGGGTGTTG CTGGACCAGA TGGGCTTCCT GGCCGCGATG GTCAAGCAGG ACAGCAGGGG
    GAGCAAGGAG ATGACGGGGA CCCCGGGCCC ATGGGCCCTG CTGGGAAGAG AGGAGATCCA
    GGTGTGGCTG GCTTGCCTGG AGCACAGGGG CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG
    CCTGGGCAGA TGGGACCCCC TGGCAAGCGA GGGACAGAAG GCGGCATGGG GCTTCCTGGG
    AACCAGGGGG AGCCTGGATC CAAAGGCCAG CCGGGTGACT CTGGTGAGAT GGGATTCCCA
    GGAATGGCTG GCCTCTTTGG ACCCAAGGGC CCCCAAGGAG ACGTCGGTTT CAAAGGCATC
    CAGGGCCCTC GGGGGCCTCC TGGCTTGATG GGAAAGGAAG GTATCATCGG GCCCCTGGGA
    ATCCTGGGAC CTTCGGGACG CCAGGGTCCA AAGGGCGACA AAGGCAGCCG CGGGGACTTG
    GGATTGCAAG GGTCCTCCCG GTCCCAGAGG GCAGCCCGGC CCACCGGGTC CTCCAGGGAG
    CACTATCCAC TTCGTAAGTT GGAGAACTTC CTCTGCGGTC TACTTGGACA ACAAGATGAC
    CTTGGGGCAG CTTTCCAGAC GTGGATGGAT ACAAGTGGAG CACTGAGAGC AGAGGGTTAC
    AGCCATCCGG ACCGGCTGGT GCTGGACCAG GGAGGGGAGA TCTTTAAAAC CTTACACTAC
    CTCAGCAACC TCATCCAGAG CATTAAGACG CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG
    GTCTGCCGGG ACCTCATGGA CTGTGAGCAG AAGATGGTGG ATGGTACCTA CTGGGTGGAT
    CCTAACCTTG GCTGCTCGTC CGACACCATC GAAGTCTCCT GCAACTTCAC ACATGGTGGA
    CAGACGTGTC TCAAGCCCAT CACGGCCTCC AAGGTGGAGT TTGCCATCAG CCGGGTCCAG
    ATGAATTTCC TGCACCTGCT AAGCTCGGAG GTGACACAGC AGATCACCAT CCACTGCCTT
    AACATGACCG TGTGGCAGGA GGGCACCGGG CAGACCCCAG CTAAGCAGGC TGTGCGCTTC
    CGGGCCTGGA ACGGGCAGAT CTTTGAGGCC GGGGGTCAGT TCAGTCCAGA AGTGTCCATG
    GATGGCTGCA AGATCCAGGA TGGCCGCTGG CATCAGACGC TCTTCACCTT CCGGACCCAG
    GACCCCCAGC AGCTGCCCAT TGTCGGCGTG GACAACCTCC CTCCCGCCTC ATCAGGGAAG
    CAGTACCGCC TCGAAGTTGG ACCTGCATGC TTCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851449.1
    CDS486..5984
    Translation

    Target ORF information:

    RefSeq Version XM_024995681.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGCC ATCCTGGAAT CCCAGGTGTG
    GTGGGAAACC CGGGAGAGCC TGGACCTCCG GGTCCTCCAG GATCTCGAGG CCCACCGGGC
    ATGAGGGGAG CAAAGGGACG CCGGGGACCC CGAGGACCAG ACGGACCAGC TGGGGAGCAG
    GGGTCCAAGG GCCTGAAGGG CCCGCCAGGA CCACAGGGCA GACTGGGCCA GCCCGGGCAG
    CAGGGCGTGG CTGGCGAGCG AGGGCACTTG GGCACACGAG GCTTTCCTGG CCTCCGGGGT
    CCTTCCGGTC CCCCAGGCGC CAAGGGCCTC CCAGGAGAAC CGGGCCCTCA GGGACCCCAG
    GGGCCAATTG GCCCTCCCGG AGAGATGGGA CCCAAGGGAC CACCAGGTGC ATTGGGAGAA
    CTGGGCCTTC CTGGGGAAGC TGGCATGAAG GGTGACCTTG GGCCTCTGGG CATCCCTGGG
    GAGCAGGGCC TCATTGGGCA GCGGGGAGAG CCAGGCCTCG AGGGTGACAG TGGCCCTGCA
    GGGCCTGACG GGCTGAAGGG GGACAGGGGT GACCCCGGGC CTGACGGTGA ACGTGGCGAG
    AAGGGCCAAG AGGGACTGAA GGGCGAGGAC GGGCCCCCCG GCCCCCCTGG CATCACTGGC
    ATCCGGGGTC CTGAAGGAAA ACCAGGGAAG CAAGGCGAGA AGGGCCGGAC GGGAGCCAAG
    GGTGCCAAGG GCTACCAAGG ACAGCTGGGT GAGATGGGCG TCCCTGGAGA CCCCGGACCC
    CCTGGCACCC CAGGCCCTAA AGGGTCCCGG GGCAGCCTGG GACCAACGGG TGCTCCAGGA
    CGGATGGGGG CCCAAGGAGA GCCGGGACTG GCTGGTTACG ACGGACACAA AGGCATCATG
    GGACCCCTCG GACCTCCTGG ACCAAAGGGT GAAAAGGGGG AGCAGGGCGA GGACGGCAAG
    GCTGAGGGGC CCCCTGGACC ACCTGGAGAT CGGGGCCCTG TGGGTGATCG AGGAGACCGT
    GGCGAACCAG GGGACCCTGG ATACCCTGGA CAGGAGGGTG TACAAGGCCT CCGTGGAAAT
    CCAGGCCAGC AGGGCCAACC CGGGCATCCG GGACCCCGGG GACGGCCGGG CCCCAAAGGA
    TCGAAAGGGG AAGAGGGGCC AAAGGGAAAG CAAGGCAAGG CTGGGGCGCC AGGACGGAGG
    GGGATCCAGG GCCTGCAGGG GCTCCCAGGG CCTCGGGGCG TGGTGGGGAG ACAGGGCCCC
    GAGGGTGTTG CTGGACCAGA TGGGCTTCCT GGCCGCGATG GTCAAGCAGG ACAGCAGGGG
    GAGCAAGGAG ATGACGGGGA CCCCGGGCCC ATGGGCCCTG CTGGGAAGAG AGGAGATCCA
    GGTGTGGCTG GCTTGCCTGG AGCACAGGGG CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG
    CCTGGGCAGA TGGGACCCCC TGGCAAGCGA GGGACAGAAG GCGGCATGGG GCTTCCTGGG
    AACCAGGGGG AGCCTGGATC CAAAGGCCAG CCGGGTGACT CTGGTGAGAT GGGATTCCCA
    GGAATGGCTG GCCTCTTTGG ACCCAAGGGC CCCCAAGGAG ACGTCGGTTT CAAAGGCATC
    CAGGGCCCTC GGGGGCCTCC TGGCTTGATG GGAAAGGAAG GTATCATCGG GCCCCTGGGA
    ATCCTGGGAC CTTCGGGACG CCAGGGTCCA AAGGGCGACA AAGGCAGCCG CGGGGACTTG
    GGATTGCAAG GGTCCTCCCG GTCCCAGAGG GCAGCCCGGC CCACCGGGTC CTCCAGGGAG
    CACTATCCAC TTCGTAAGTT GGAGAACTTC CTCTGCGGTC TACTTGGACA ACAAGATGAC
    CTTGGGGCAG CTTTCCAGAC GTGGATGGAT ACAAGTGGAG CACTGAGAGC AGAGGGTTAC
    AGCCATCCGG ACCGGCTGGT GCTGGACCAG GGAGGGGAGA TCTTTAAAAC CTTACACTAC
    CTCAGCAACC TCATCCAGAG CATTAAGACG CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG
    GTCTGCCGGG ACCTCATGGA CTGTGAGCAG AAGATGGTGG ATGGTACCTA CTGGGTGGAT
    CCTAACCTTG GCTGCTCGTC CGACACCATC GAAGTCTCCT GCAACTTCAC ACATGGTGGA
    CAGACGTGTC TCAAGCCCAT CACGGCCTCC AAGGTGGAGT TTGCCATCAG CCGGGTCCAG
    ATGAATTTCC TGCACCTGCT AAGCTCGGAG GTGACACAGC AGATCACCAT CCACTGCCTT
    AACATGACCG TGTGGCAGGA GGGCACCGGG CAGACCCCAG CTAAGCAGGC TGTGCGCTTC
    CGGGCCTGGA ACGGGCAGAT CTTTGAGGCC GGGGGTCAGT TCAGTCCAGA AGTGTCCATG
    GATGGCTGCA AGATCCAGGA TGGCCGCTGG CATCAGACGC TCTTCACCTT CCGGACCCAG
    GACCCCCAGC AGCTGCCCAT TGTCGGCGTG GACAACCTCC CTCCCGCCTC ATCAGGGAAG
    CAGTACCGCC TCGAAGTTGG ACCTGCATGC TTCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237056
    Clone ID Related Accession (Same CDS sequence) XM_024995682.1
    Accession Version XM_024995682.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GCGACATGGG CTTGCCCGGG
    CTCACTGGGA ATCCAGGACC CCTTGGACGA AAGGGACAGA AGGGTTATCC TGGACCAACG
    GGGCACCCCG GAGAACAGGG GCAGCCAGGA CCCGAGGGTA GCCCAGGGGC CAAAGGTTAC
    CCTGGCAGGC AGGGGCTGCC TGGACCTGTA GGAGACCCTG GGCCCAAAGG TAGCCGGGGC
    TACATTGGAC TCCCGGGGCT CTTCGGCCTG CCAGGGTCTG ATGGAGAGCG AGGTCTGCCT
    GGCGTTCCTG GCAAGAGGGG CAAGATGGGT AGGCCGGGGT TTCCTGGAGA CTTTGGGGAA
    AGAGGCCCTC CAGGACTGGA TGGAAACCCT GGAGAACTGG GCCTGCCAGG GCCCCCAGGA
    GTCCCCGGCC TCATTGGCGA CATGGGAACA TTGGGTCCGA TTGGCTACCC AGGACCCAAG
    GGCATGAAGG GACTGATGGG AAACGTGGGG GAACCCGGAC TGAAAGGTGA TAAGGGTGAA
    CAAGGGGTTC CAGGTGTGTC AGGAGACGCC GGATTCCAAG GAGACAAGGG GAGCCAGGGC
    TTGCAAGGGT TCCCTGGCGC ACGGGGGAAG CCAGGGCCAG TGGGCAAAGT TGGAGACAAA
    GGATCCATTG GGTTTCCTGG GCCCCCGGGA CCTGAGGGAT TCCCTGGAGA CATTGGTCCC
    CCTGGGGACA ACGGCCCAGA AGGCGTGAAG GGTAAGCCTG GAGCTCGAGG CCTGCCAGGA
    CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT GAGGGGCCCA TGGGGCCACC AGGGGTCCCT
    GGCTTGGAGG GTCAACCCGG CAGGAAGGGG TTTCCTGGAA GACCTGGCCC GGATGGCTTG
    AAGGGCGAGC CAGGCGATGC TGGACGGCCG GGGCCTGTGG GTGAGCAGGG ACTTCTGGGA
    TTCGTTGGTC TGGTCGGAGA GCCAGGAATC ATGGGAGAAA AGGGTGACCG GGGCATGATG
    GGACCCCCAG GCGCGCCCGG ACCCAAGGGG TCGATGGGCC ATCCTGGAAT CCCAGGTGTG
    GTGGGAAACC CGGGAGAGCC TGGACCTCCG GGTCCTCCAG GATCTCGAGG CCCACCGGGC
    ATGAGGGGAG CAAAGGGACG CCGGGGACCC CGAGGACCAG ACGGACCAGC TGGGGAGCAG
    GGGTCCAAGG GCCTGAAGGG CCCGCCAGGA CCACAGGGCA GACTGGGCCA GCCCGGGCAG
    CAGGGCGTGG CTGGCGAGCG AGGGCACTTG GGCACACGAG GCTTTCCTGG CCTCCGGGGT
    CCTTCCGGTC CCCCAGGCGC CAAGGGCCTC CCAGGAGAAC CGGGCCCTCA GGGACCCCAG
    GGGCCAATTG GCCCTCCCGG AGAGATGGGA CCCAAGGGAC CACCAGGTGC ATTGGGAGAA
    CTGGGCCTTC CTGGGGAAGC TGGCATGAAG GGTGACCTTG GGCCTCTGGG CATCCCTGGG
    GAGCAGGGCC TCATTGGGCA GCGGGGAGAG CCAGGCCTCG AGGGTGACAG TGGCCCTGCA
    GGGCCTGACG GGCTGAAGGG GGACAGGGGT GACCCCGGGC CTGACGGTGA ACGTGGCGAG
    AAGGGCCAAG AGGGACTGAA GGGCGAGGAC GGGCCCCCCG GCCCCCCTGG CATCACTGGC
    ATCCGGGGTC CTGAAGGAAA ACCAGGGAAG CAAGGCGAGA AGGGCCGGAC GGGAGCCAAG
    GGTGCCAAGG GCTACCAAGG ACAGCTGGGT GAGATGGGCG TCCCTGGAGA CCCCGGACCC
    CCTGGCACCC CAGGCCCTAA AGGGTCCCGG GGCAGCCTGG GACCAACGGG TGCTCCAGGA
    CGGATGGGGG CCCAAGGAGA GCCGGGACTG GCTGGTTACG ACGGACACAA AGGCATCATG
    GGACCCCTCG GACCTCCTGG ACCAAAGGGT GAAAAGGGGG AGCAGGGCGA GGACGGCAAG
    GCTGAGGGGC CCCCTGGACC ACCTGGAGAT CGGGGCCCTG TGGGTGATCG AGGAGACCGT
    GGCGAACCAG GGGACCCTGG ATACCCTGGA CAGGAGGGTG TACAAGGCCT CCGTGGAAAT
    CCAGGCCAGC AGGGCCAACC CGGGCATCCG GGACCCCGGG GACGGCCGGG CCCCAAAGGA
    TCGAAAGGGG AAGAGGGGCC AAAGGGAAAG CAAGGCAAGG CTGGGGCGCC AGGACGGAGG
    GGGATCCAGG GCCTGCAGGG GCTCCCAGGG CCTCGGGGCG TGGTGGGGAG ACAGGGCCCC
    GAGGGTGTTG CTGGACCAGA TGGGCTTCCT GGCCGCGATG GTCAAGCAGG ACAGCAGGGG
    GAGCAAGGAG ATGACGGGGA CCCCGGGCCC ATGGGCCCTG CTGGGAAGAG AGGAGATCCA
    GGTGTGGCTG GCTTGCCTGG AGCACAGGGG CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG
    CCTGGGCAGA TGGGACCCCC TGGCAAGCGA GGGACAGAAG GCGGCATGGG GCTTCCTGGG
    AACCAGGGGG AGCCTGGATC CAAAGGCCAG CCGGGTGACT CTGGTGAGAT GGGATTCCCA
    GGAATGGCTG GCCTCTTTGG ACCCAAGGGC CCCCAAGGAG ACGTCGGTTT CAAAGGCATC
    CAGGGCCCTC GGGGGCCTCC TGGCTTGATG GGAAAGGAAG GTATCATCGG GCCCCTGGGA
    ATCCTGGGAC CTTCGGGACG CCAGGGTCCA AAGGGCGACA AAGGCAGCCG CGGGGACTTG
    GGATTGCAAG GGTCCTCCCG GTCCCAGAGG GCAGCCCGGC CCACCGGGTC CTCCAGGGAG
    CACTATCCAC TTCGTAAGTT GGAGAACTTC CTCTGCGGTC TACTTGGACA ACAAGATGAC
    CTTGGGGCAG CTTTCCAGAC GTGGATGGAT ACAAGTGGAG CACTGAGAGC AGAGGGTTAC
    AGCCATCCGG ACCGGCTGGT GCTGGACCAG GGAGGGGAGA TCTTTAAAAC CTTACACTAC
    CTCAGCAACC TCATCCAGAG CATTAAGACG CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG
    GTCTGCCGGG ACCTCATGGA CTGTGAGCAG AAGATGGTGG ATGGTACCTA CTGGGTGGAT
    CCTAACCTTG GCTGCTCGTC CGACACCATC GAAGTCTCCT GCAACTTCAC ACATGGTGGA
    CAGACGTGTC TCAAGCCCAT CACGGCCTCC AAGGTGGAGT TTGCCATCAG CCGGGTCCAG
    ATGAATTTCC TGCACCTGCT AAGCTCGGAG GTGACACAGC AGATCACCAT CCACTGCCTT
    AACATGACCG TGTGGCAGGA GGGCACCGGG CAGACCCCAG CTAAGCAGGC TGTGCGCTTC
    CGGGCCTGGA ACGGGCAGAT CTTTGAGGCC GGGGGTCAGT TCAGTCCAGA AGTGTCCATG
    GATGGCTGCA AGATCCAGGA TGGCCGCTGG CATCAGACGC TCTTCACCTT CCGGACCCAG
    GACCCCCAGC AGCTGCCCAT TGTCGGCGTG GACAACCTCC CTCCCGCCTC ATCAGGGAAG
    CAGTACCGCC TCGAAGTTGG ACCTGCATGC TTCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851450.1
    CDS486..5984
    Translation

    Target ORF information:

    RefSeq Version XM_024995682.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995682.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GCGACATGGG CTTGCCCGGG
    CTCACTGGGA ATCCAGGACC CCTTGGACGA AAGGGACAGA AGGGTTATCC TGGACCAACG
    GGGCACCCCG GAGAACAGGG GCAGCCAGGA CCCGAGGGTA GCCCAGGGGC CAAAGGTTAC
    CCTGGCAGGC AGGGGCTGCC TGGACCTGTA GGAGACCCTG GGCCCAAAGG TAGCCGGGGC
    TACATTGGAC TCCCGGGGCT CTTCGGCCTG CCAGGGTCTG ATGGAGAGCG AGGTCTGCCT
    GGCGTTCCTG GCAAGAGGGG CAAGATGGGT AGGCCGGGGT TTCCTGGAGA CTTTGGGGAA
    AGAGGCCCTC CAGGACTGGA TGGAAACCCT GGAGAACTGG GCCTGCCAGG GCCCCCAGGA
    GTCCCCGGCC TCATTGGCGA CATGGGAACA TTGGGTCCGA TTGGCTACCC AGGACCCAAG
    GGCATGAAGG GACTGATGGG AAACGTGGGG GAACCCGGAC TGAAAGGTGA TAAGGGTGAA
    CAAGGGGTTC CAGGTGTGTC AGGAGACGCC GGATTCCAAG GAGACAAGGG GAGCCAGGGC
    TTGCAAGGGT TCCCTGGCGC ACGGGGGAAG CCAGGGCCAG TGGGCAAAGT TGGAGACAAA
    GGATCCATTG GGTTTCCTGG GCCCCCGGGA CCTGAGGGAT TCCCTGGAGA CATTGGTCCC
    CCTGGGGACA ACGGCCCAGA AGGCGTGAAG GGTAAGCCTG GAGCTCGAGG CCTGCCAGGA
    CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT GAGGGGCCCA TGGGGCCACC AGGGGTCCCT
    GGCTTGGAGG GTCAACCCGG CAGGAAGGGG TTTCCTGGAA GACCTGGCCC GGATGGCTTG
    AAGGGCGAGC CAGGCGATGC TGGACGGCCG GGGCCTGTGG GTGAGCAGGG ACTTCTGGGA
    TTCGTTGGTC TGGTCGGAGA GCCAGGAATC ATGGGAGAAA AGGGTGACCG GGGCATGATG
    GGACCCCCAG GCGCGCCCGG ACCCAAGGGG TCGATGGGCC ATCCTGGAAT CCCAGGTGTG
    GTGGGAAACC CGGGAGAGCC TGGACCTCCG GGTCCTCCAG GATCTCGAGG CCCACCGGGC
    ATGAGGGGAG CAAAGGGACG CCGGGGACCC CGAGGACCAG ACGGACCAGC TGGGGAGCAG
    GGGTCCAAGG GCCTGAAGGG CCCGCCAGGA CCACAGGGCA GACTGGGCCA GCCCGGGCAG
    CAGGGCGTGG CTGGCGAGCG AGGGCACTTG GGCACACGAG GCTTTCCTGG CCTCCGGGGT
    CCTTCCGGTC CCCCAGGCGC CAAGGGCCTC CCAGGAGAAC CGGGCCCTCA GGGACCCCAG
    GGGCCAATTG GCCCTCCCGG AGAGATGGGA CCCAAGGGAC CACCAGGTGC ATTGGGAGAA
    CTGGGCCTTC CTGGGGAAGC TGGCATGAAG GGTGACCTTG GGCCTCTGGG CATCCCTGGG
    GAGCAGGGCC TCATTGGGCA GCGGGGAGAG CCAGGCCTCG AGGGTGACAG TGGCCCTGCA
    GGGCCTGACG GGCTGAAGGG GGACAGGGGT GACCCCGGGC CTGACGGTGA ACGTGGCGAG
    AAGGGCCAAG AGGGACTGAA GGGCGAGGAC GGGCCCCCCG GCCCCCCTGG CATCACTGGC
    ATCCGGGGTC CTGAAGGAAA ACCAGGGAAG CAAGGCGAGA AGGGCCGGAC GGGAGCCAAG
    GGTGCCAAGG GCTACCAAGG ACAGCTGGGT GAGATGGGCG TCCCTGGAGA CCCCGGACCC
    CCTGGCACCC CAGGCCCTAA AGGGTCCCGG GGCAGCCTGG GACCAACGGG TGCTCCAGGA
    CGGATGGGGG CCCAAGGAGA GCCGGGACTG GCTGGTTACG ACGGACACAA AGGCATCATG
    GGACCCCTCG GACCTCCTGG ACCAAAGGGT GAAAAGGGGG AGCAGGGCGA GGACGGCAAG
    GCTGAGGGGC CCCCTGGACC ACCTGGAGAT CGGGGCCCTG TGGGTGATCG AGGAGACCGT
    GGCGAACCAG GGGACCCTGG ATACCCTGGA CAGGAGGGTG TACAAGGCCT CCGTGGAAAT
    CCAGGCCAGC AGGGCCAACC CGGGCATCCG GGACCCCGGG GACGGCCGGG CCCCAAAGGA
    TCGAAAGGGG AAGAGGGGCC AAAGGGAAAG CAAGGCAAGG CTGGGGCGCC AGGACGGAGG
    GGGATCCAGG GCCTGCAGGG GCTCCCAGGG CCTCGGGGCG TGGTGGGGAG ACAGGGCCCC
    GAGGGTGTTG CTGGACCAGA TGGGCTTCCT GGCCGCGATG GTCAAGCAGG ACAGCAGGGG
    GAGCAAGGAG ATGACGGGGA CCCCGGGCCC ATGGGCCCTG CTGGGAAGAG AGGAGATCCA
    GGTGTGGCTG GCTTGCCTGG AGCACAGGGG CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG
    CCTGGGCAGA TGGGACCCCC TGGCAAGCGA GGGACAGAAG GCGGCATGGG GCTTCCTGGG
    AACCAGGGGG AGCCTGGATC CAAAGGCCAG CCGGGTGACT CTGGTGAGAT GGGATTCCCA
    GGAATGGCTG GCCTCTTTGG ACCCAAGGGC CCCCAAGGAG ACGTCGGTTT CAAAGGCATC
    CAGGGCCCTC GGGGGCCTCC TGGCTTGATG GGAAAGGAAG GTATCATCGG GCCCCTGGGA
    ATCCTGGGAC CTTCGGGACG CCAGGGTCCA AAGGGCGACA AAGGCAGCCG CGGGGACTTG
    GGATTGCAAG GGTCCTCCCG GTCCCAGAGG GCAGCCCGGC CCACCGGGTC CTCCAGGGAG
    CACTATCCAC TTCGTAAGTT GGAGAACTTC CTCTGCGGTC TACTTGGACA ACAAGATGAC
    CTTGGGGCAG CTTTCCAGAC GTGGATGGAT ACAAGTGGAG CACTGAGAGC AGAGGGTTAC
    AGCCATCCGG ACCGGCTGGT GCTGGACCAG GGAGGGGAGA TCTTTAAAAC CTTACACTAC
    CTCAGCAACC TCATCCAGAG CATTAAGACG CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG
    GTCTGCCGGG ACCTCATGGA CTGTGAGCAG AAGATGGTGG ATGGTACCTA CTGGGTGGAT
    CCTAACCTTG GCTGCTCGTC CGACACCATC GAAGTCTCCT GCAACTTCAC ACATGGTGGA
    CAGACGTGTC TCAAGCCCAT CACGGCCTCC AAGGTGGAGT TTGCCATCAG CCGGGTCCAG
    ATGAATTTCC TGCACCTGCT AAGCTCGGAG GTGACACAGC AGATCACCAT CCACTGCCTT
    AACATGACCG TGTGGCAGGA GGGCACCGGG CAGACCCCAG CTAAGCAGGC TGTGCGCTTC
    CGGGCCTGGA ACGGGCAGAT CTTTGAGGCC GGGGGTCAGT TCAGTCCAGA AGTGTCCATG
    GATGGCTGCA AGATCCAGGA TGGCCGCTGG CATCAGACGC TCTTCACCTT CCGGACCCAG
    GACCCCCAGC AGCTGCCCAT TGTCGGCGTG GACAACCTCC CTCCCGCCTC ATCAGGGAAG
    CAGTACCGCC TCGAAGTTGG ACCTGCATGC TTCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237057
    Clone ID Related Accession (Same CDS sequence) XM_024995683.1
    Accession Version XM_024995683.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA 
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGGTCCT CCCGGTCCCA GAGGGCAGCC CGGCCCACCG GGTCCTCCAG GGAGCACTAT
    CCACTTCGTA AGTTGGAGAA CTTCCTCTGC GGTCTACTTG GACAACAAGA TGACCTTGGG
    GCAGCTTTCC AGACGTGGAT GGATACAAGT GGAGCACTGA GAGCAGAGGG TTACAGCCAT
    CCGGACCGGC TGGTGCTGGA CCAGGGAGGG GAGATCTTTA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACGCCCCTG GGCACCAAAG AGAACCCCGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAAGATG GTGGATGGTA CCTACTGGGT GGATCCTAAC
    CTTGGCTGCT CGTCCGACAC CATCGAAGTC TCCTGCAACT TCACACATGG TGGACAGACG
    TGTCTCAAGC CCATCACGGC CTCCAAGGTG GAGTTTGCCA TCAGCCGGGT CCAGATGAAT
    TTCCTGCACC TGCTAAGCTC GGAGGTGACA CAGCAGATCA CCATCCACTG CCTTAACATG
    ACCGTGTGGC AGGAGGGCAC CGGGCAGACC CCAGCTAAGC AGGCTGTGCG CTTCCGGGCC
    TGGAACGGGC AGATCTTTGA GGCCGGGGGT CAGTTCAGTC CAGAAGTGTC CATGGATGGC
    TGCAAGATCC AGGATGGCCG CTGGCATCAG ACGCTCTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATTGTCGG CGTGGACAAC CTCCCTCCCG CCTCATCAGG GAAGCAGTAC
    CGCCTCGAAG TTGGACCTGC ATGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851451.1
    CDS18..5510
    Translation

    Target ORF information:

    RefSeq Version XM_024995683.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995683.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA 
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGGTCCT CCCGGTCCCA GAGGGCAGCC CGGCCCACCG GGTCCTCCAG GGAGCACTAT
    CCACTTCGTA AGTTGGAGAA CTTCCTCTGC GGTCTACTTG GACAACAAGA TGACCTTGGG
    GCAGCTTTCC AGACGTGGAT GGATACAAGT GGAGCACTGA GAGCAGAGGG TTACAGCCAT
    CCGGACCGGC TGGTGCTGGA CCAGGGAGGG GAGATCTTTA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACGCCCCTG GGCACCAAAG AGAACCCCGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAAGATG GTGGATGGTA CCTACTGGGT GGATCCTAAC
    CTTGGCTGCT CGTCCGACAC CATCGAAGTC TCCTGCAACT TCACACATGG TGGACAGACG
    TGTCTCAAGC CCATCACGGC CTCCAAGGTG GAGTTTGCCA TCAGCCGGGT CCAGATGAAT
    TTCCTGCACC TGCTAAGCTC GGAGGTGACA CAGCAGATCA CCATCCACTG CCTTAACATG
    ACCGTGTGGC AGGAGGGCAC CGGGCAGACC CCAGCTAAGC AGGCTGTGCG CTTCCGGGCC
    TGGAACGGGC AGATCTTTGA GGCCGGGGGT CAGTTCAGTC CAGAAGTGTC CATGGATGGC
    TGCAAGATCC AGGATGGCCG CTGGCATCAG ACGCTCTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATTGTCGG CGTGGACAAC CTCCCTCCCG CCTCATCAGG GAAGCAGTAC
    CGCCTCGAAG TTGGACCTGC ATGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237058
    Clone ID Related Accession (Same CDS sequence) XM_024995684.1
    Accession Version XM_024995684.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGTCCGA GGGGTCCTCC CGGTCCCAGA GGGCAGCCCG GCCCACCGGG TCCTCCAGGG
    AGCACTATCC ACTTCGTAAG TTGGAGAACT TCCTCTGCGG TCTACTTGGA CAACAAGATG
    ACCTTGGGGC AGCTTTCCAG ACGTGGATGG ATACAAGTGG AGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851452.1
    CDS488..5395
    Translation

    Target ORF information:

    RefSeq Version XM_024995684.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995684.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGTCCGA GGGGTCCTCC CGGTCCCAGA GGGCAGCCCG GCCCACCGGG TCCTCCAGGG
    AGCACTATCC ACTTCGTAAG TTGGAGAACT TCCTCTGCGG TCTACTTGGA CAACAAGATG
    ACCTTGGGGC AGCTTTCCAG ACGTGGATGG ATACAAGTGG AGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36307
    Clone ID Related Accession (Same CDS sequence) NM_001206680.1
    Accession Version NM_001206680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXVII) chain
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03130469.1, AAFC03120155.1, AAFC03126695.1, AAFC03062330.1, AAFC03062331.1 and BC118144.1. On or before May 4, 2011 this sequence version replaced XM_602219.5, XM_002689949.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_116277.2. COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGTCCGA GGGGTCCTCC CGGTCCCAGA GGGCAGCCCG GCCCACCGGG TCCTCCAGGG
    AGCACTATCC ACTTCCAACA AGATGACCTT GGGGCAGCTT TCCAGACGTG GATGGATACA
    AGTGGAGCAC TGAGAGCAGA GGGTTACAGC CATCCGGACC GGCTGGTGCT GGACCAGGGA
    GGGGAGATCT TTAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACGCCC
    CTGGGCACCA AAGAGAACCC CGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAAG
    ATGGTGGATG GTACCTACTG GGTGGATCCT AACCTTGGCT GCTCGTCCGA CACCATCGAA
    GTCTCCTGCA ACTTCACACA TGGTGGACAG ACGTGTCTCA AGCCCATCAC GGCCTCCAAG
    GTGGAGTTTG CCATCAGCCG GGTCCAGATG AATTTCCTGC ACCTGCTAAG CTCGGAGGTG
    ACACAGCAGA TCACCATCCA CTGCCTTAAC ATGACCGTGT GGCAGGAGGG CACCGGGCAG
    ACCCCAGCTA AGCAGGCTGT GCGCTTCCGG GCCTGGAACG GGCAGATCTT TGAGGCCGGG
    GGTCAGTTCA GTCCAGAAGT GTCCATGGAT GGCTGCAAGA TCCAGGATGG CCGCTGGCAT
    CAGACGCTCT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATTGT CGGCGTGGAC
    AACCTCCCTC CCGCCTCATC AGGGAAGCAG TACCGCCTCG AAGTTGGACC TGCATGCTTC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193609.1
    CDS1..5526
    Translation

    Target ORF information:

    RefSeq Version NM_001206680.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen, type XXVII, alpha 1 (COL27A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGAGCGG GATCGGCGCG GGGGACCCGA GGCACAGCAG CGGCGGCGGC GGCGCAAGGG 
    GGGGGGTTTC TCTTCACCTG GATCTTAGTC TCGCTCACCT GTCACCTGGC CTCCACCCAA
    GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CAGCTGGGCC TCAGCTGGAC GAAGGCGGTG
    GGCGGCCGGA GCCCCCCGCC CCCAGGGGTC ATCCCGTTCC AGACGGGCTT CATCTTCACG
    CAGCGGGCCC GGCTCCAGGC TCCCACGGCT GCCGTCCTCC CCGCCTCCCT GGGCACGGAG
    CTGGCGCTGG TGCTCAGCCT CTGCTCCCAC CGGGTGAACC ATGCCTTCCT CTTCGCCGTC
    CGCAGCCGGA AGCGCAAGCT GCAGCTGGGC CTGCAGTTCC TCCCCGGCAA GACTGTGGTC
    CACCTGGGGC CCCGGCGCTC CGTGGCCTTC GACCTCGACG TCCACGACGG GCGCTGGCAC
    CACCTGGCCC TGGAGCTCCG CGGCCGCACG GTCACCCTGG TGACGGCCTG CGGGCAGCAC
    CGGGTGCCCG TCTCGCTGCC TTTCCACAGG GACCCAGCGC TCGACCCTGA CGGCTTGTTC
    CTCTTTGGGA AGATGAGCCC GCACGCGGTC CAGTTCGAGG GGGCCCTGTG CCAGTTCAGT
    ATCTACCCCG TGGCGCAGGT CGCTCACAAT TACTGTACCC ACCTGAGAAA GCAGTGTGGA
    CAGGCTGACA CCTACCGGCC CCAGCTGGGA CCCTTCTTCC CCCGAGACTC TGGCACACTC
    CTCACCTTCC AGACCGACCT CGCCCGGTTG GGCCTGGAGA ATCTGACCAC TGCCACGCCA
    GCCCTGGGGT CACGGCCGGC AGGCAGGGGG CCTGGGGTGA CTGTGGTGCC TGCTGTGCCC
    GTCAAGCCCA TGAGGACTAG CACCACACGC CCGCCCCAGC CCATCACATC CCATCCAGCC
    TGGACCCCTC TGTCCCCTGC CAAGCTATCA GTCAGTGAAG GGCCTCCTCC CGTGTCCCCG
    GTCTCTCCTG CCAGCTCCCC CAGACCTGTC CAGCCATTGC AGAAGAACAC AGCCACTAAG
    ACCTCCAAAA GTCACCCCGC CACACCTTCA GCCCTTTCTC CTTCAATTGC CCCAGTCAGA
    AGCCCCCGCC CCACCCAGAA GACAGCTCTG CCTGCCTTCA CAAAGCCAGC CCCACCCACC
    AAGAAGCCGG TGCTACCTAC TTCCCAGCCC ATTCCTGCCA AAGTCTCCCG TCCCACAGTG
    AAGCCGATCC AGAGGAACCC CGGCATGCCC AGACTCCCGC CACCCAGTGC CCGGCCCCTA
    CCTCCAGCTG CTGCCTCCTC TAAAAAGCCT TTGCCCCCAG TAGTCCAGGC TGAGGCCAAG
    ATGAGTAGTC GTGCCAGTGA GCCGGCTCCT GCCCATACCA GCACACACAG ACCTCCCCCA
    CCCACAGTCC CGCCCCCATC ACCTGTCCCC AGTCCTAGCC CTCCCAGGAC CGCTCGGCCA
    CCAGCCACAG CGATGCCTCC CACTCTCGCT CCTGGTTCAG CCTCCACTGG AAGCAAGAAA
    CCCACTGGAT CAGAAGCCAC AAAGAAAGCC AGACCCCGGA AGCCCGTCCC ACTCAGATCT
    GGGAAGGCAG CCCGGGATGT CCCGCTAAAC GATCCAACAA GGGGGTCCAG CCCCAGACAG
    CCCCGGACCA GGCAGCAGAC CACCCCGGCC CCAGCGCTGG CCCCGGCGCG ATTCCTGGCC
    TCCAGCCCCC AGCCCATGAG TACTGGCTAT TCGTTCTTCC ACCTGGTGGG ACCCACACCT
    TTCCTGCTGC TGATGGGACC TCCAGGACCC AAGGGAGACT GTGGTTTGCC GGGTCCCCCT
    GGACTGCCTG GGCTTCCTGG ACCCCCTGGC GCACGGGGCC CTCGGGGTCC TCCTGGGCCT
    TATGGAAATC CAGGCCTTCC TGGCCCCCCT GGAGCCAAAG GACAGAAAGG GGACCCCGGG
    CTCTCACCAG GAAAGGCCCA CGATGGGGCA AAGGGCGACA TGGGCTTGCC CGGGCTCACT
    GGGAATCCAG GACCCCTTGG ACGAAAGGGA CAGAAGGGTT ATCCTGGACC AACGGGGCAC
    CCCGGAGAAC AGGGGCAGCC AGGACCCGAG GGTAGCCCAG GGGCCAAAGG TTACCCTGGC
    AGGCAGGGGC TGCCTGGACC TGTAGGAGAC CCTGGGCCCA AAGGTAGCCG GGGCTACATT
    GGACTCCCGG GGCTCTTCGG CCTGCCAGGG TCTGATGGAG AGCGAGGTCT GCCTGGCGTT
    CCTGGCAAGA GGGGCAAGAT GGGTAGGCCG GGGTTTCCTG GAGACTTTGG GGAAAGAGGC
    CCTCCAGGAC TGGATGGAAA CCCTGGAGAA CTGGGCCTGC CAGGGCCCCC AGGAGTCCCC
    GGCCTCATTG GCGACATGGG AACATTGGGT CCGATTGGCT ACCCAGGACC CAAGGGCATG
    AAGGGACTGA TGGGAAACGT GGGGGAACCC GGACTGAAAG GTGATAAGGG TGAACAAGGG
    GTTCCAGGTG TGTCAGGAGA CGCCGGATTC CAAGGAGACA AGGGGAGCCA GGGCTTGCAA
    GGGTTCCCTG GCGCACGGGG GAAGCCAGGG CCAGTGGGCA AAGTTGGAGA CAAAGGATCC
    ATTGGGTTTC CTGGGCCCCC GGGACCTGAG GGATTCCCTG GAGACATTGG TCCCCCTGGG
    GACAACGGCC CAGAAGGCGT GAAGGGTAAG CCTGGAGCTC GAGGCCTGCC AGGACCCCGT
    GGGCAGCTGG GGCCCGAGGG AGATGAGGGG CCCATGGGGC CACCAGGGGT CCCTGGCTTG
    GAGGGTCAAC CCGGCAGGAA GGGGTTTCCT GGAAGACCTG GCCCGGATGG CTTGAAGGGC
    GAGCCAGGCG ATGCTGGACG GCCGGGGCCT GTGGGTGAGC AGGGACTTCT GGGATTCGTT
    GGTCTGGTCG GAGAGCCAGG AATCATGGGA GAAAAGGGTG ACCGGGGCAT GATGGGACCC
    CCAGGCGCGC CCGGACCCAA GGGGTCGATG GGCCATCCTG GAATCCCAGG TGTGGTGGGA
    AACCCGGGAG AGCCTGGACC TCCGGGTCCT CCAGGATCTC GAGGCCCACC GGGCATGAGG
    GGAGCAAAGG GACGCCGGGG ACCCCGAGGA CCAGACGGAC CAGCTGGGGA GCAGGGGTCC
    AAGGGCCTGA AGGGCCCGCC AGGACCACAG GGCAGACTGG GCCAGCCCGG GCAGCAGGGC
    GTGGCTGGCG AGCGAGGGCA CTTGGGCACA CGAGGCTTTC CTGGCCTCCG GGGTCCTTCC
    GGTCCCCCAG GCGCCAAGGG CCTCCCAGGA GAACCGGGCC CTCAGGGACC CCAGGGGCCA
    ATTGGCCCTC CCGGAGAGAT GGGACCCAAG GGACCACCAG GTGCATTGGG AGAACTGGGC
    CTTCCTGGGG AAGCTGGCAT GAAGGGTGAC CTTGGGCCTC TGGGCATCCC TGGGGAGCAG
    GGCCTCATTG GGCAGCGGGG AGAGCCAGGC CTCGAGGGTG ACAGTGGCCC TGCAGGGCCT
    GACGGGCTGA AGGGGGACAG GGGTGACCCC GGGCCTGACG GTGAACGTGG CGAGAAGGGC
    CAAGAGGGAC TGAAGGGCGA GGACGGGCCC CCCGGCCCCC CTGGCATCAC TGGCATCCGG
    GGTCCTGAAG GAAAACCAGG GAAGCAAGGC GAGAAGGGCC GGACGGGAGC CAAGGGTGCC
    AAGGGCTACC AAGGACAGCT GGGTGAGATG GGCGTCCCTG GAGACCCCGG ACCCCCTGGC
    ACCCCAGGCC CTAAAGGGTC CCGGGGCAGC CTGGGACCAA CGGGTGCTCC AGGACGGATG
    GGGGCCCAAG GAGAGCCGGG ACTGGCTGGT TACGACGGAC ACAAAGGCAT CATGGGACCC
    CTCGGACCTC CTGGACCAAA GGGTGAAAAG GGGGAGCAGG GCGAGGACGG CAAGGCTGAG
    GGGCCCCCTG GACCACCTGG AGATCGGGGC CCTGTGGGTG ATCGAGGAGA CCGTGGCGAA
    CCAGGGGACC CTGGATACCC TGGACAGGAG GGTGTACAAG GCCTCCGTGG AAATCCAGGC
    CAGCAGGGCC AACCCGGGCA TCCGGGACCC CGGGGACGGC CGGGCCCCAA AGGATCGAAA
    GGGGAAGAGG GGCCAAAGGG AAAGCAAGGC AAGGCTGGGG CGCCAGGACG GAGGGGGATC
    CAGGGCCTGC AGGGGCTCCC AGGGCCTCGG GGCGTGGTGG GGAGACAGGG CCCCGAGGGT
    GTTGCTGGAC CAGATGGGCT TCCTGGCCGC GATGGTCAAG CAGGACAGCA GGGGGAGCAA
    GGAGATGACG GGGACCCCGG GCCCATGGGC CCTGCTGGGA AGAGAGGAGA TCCAGGTGTG
    GCTGGCTTGC CTGGAGCACA GGGGCCCCCA GGATTCAAGG GTGAAAGTGG GTTGCCTGGG
    CAGATGGGAC CCCCTGGCAA GCGAGGGACA GAAGGCGGCA TGGGGCTTCC TGGGAACCAG
    GGGGAGCCTG GATCCAAAGG CCAGCCGGGT GACTCTGGTG AGATGGGATT CCCAGGAATG
    GCTGGCCTCT TTGGACCCAA GGGCCCCCAA GGAGACGTCG GTTTCAAAGG CATCCAGGGC
    CCTCGGGGGC CTCCTGGCTT GATGGGAAAG GAAGGTATCA TCGGGCCCCT GGGAATCCTG
    GGACCTTCGG GACGCCAGGG TCCAAAGGGC GACAAAGGCA GCCGCGGGGA CTTGGGATTG
    CAAGGTCCGA GGGGTCCTCC CGGTCCCAGA GGGCAGCCCG GCCCACCGGG TCCTCCAGGG
    AGCACTATCC ACTTCCAACA AGATGACCTT GGGGCAGCTT TCCAGACGTG GATGGATACA
    AGTGGAGCAC TGAGAGCAGA GGGTTACAGC CATCCGGACC GGCTGGTGCT GGACCAGGGA
    GGGGAGATCT TTAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACGCCC
    CTGGGCACCA AAGAGAACCC CGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAAG
    ATGGTGGATG GTACCTACTG GGTGGATCCT AACCTTGGCT GCTCGTCCGA CACCATCGAA
    GTCTCCTGCA ACTTCACACA TGGTGGACAG ACGTGTCTCA AGCCCATCAC GGCCTCCAAG
    GTGGAGTTTG CCATCAGCCG GGTCCAGATG AATTTCCTGC ACCTGCTAAG CTCGGAGGTG
    ACACAGCAGA TCACCATCCA CTGCCTTAAC ATGACCGTGT GGCAGGAGGG CACCGGGCAG
    ACCCCAGCTA AGCAGGCTGT GCGCTTCCGG GCCTGGAACG GGCAGATCTT TGAGGCCGGG
    GGTCAGTTCA GTCCAGAAGT GTCCATGGAT GGCTGCAAGA TCCAGGATGG CCGCTGGCAT
    CAGACGCTCT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATTGT CGGCGTGGAC
    AACCTCCCTC CCGCCTCATC AGGGAAGCAG TACCGCCTCG AAGTTGGACC TGCATGCTTC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.