PLCH1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010824413.2	XP_010822715.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_010801464.2	XP_010799766.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015472920.1	XP_015328406.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_015472924.1	XP_015328410.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_010824408.2	XP_010822710.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015462097.1	XP_015317583.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015462101.1	XP_015317587.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_010801461.2	XP_010799763.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_010801462.2	XP_010799764.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015472917.1	XP_015328403.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_024995857.1	XP_024851625.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015472919.1	XP_015328405.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_010824409.2	XP_010822711.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_010824411.2	XP_010822713.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015462100.1	XP_015317586.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_015472914.1	XP_015328400.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_024995849.1	XP_024851617.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_024995851.1	XP_024851619.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015472917.2	XP_015328403.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_010801466.2	XP_010799768.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_015462098.1	XP_015317584.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015462099.1	XP_015317585.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_024995863.1	XP_024851631.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219229	XM_024995857.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219229	XM_024995849.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219229	XM_024995851.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219230	XM_024995863.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219231	XM_015472917.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa02642	XM_010824408.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_010824409.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015462099.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015462098.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_010801462.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015472919.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015472914.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015462097.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_010801464.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_010801461.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_010824411.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02642	XM_015472917.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48661	XM_015462100.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48661	XM_015472920.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48662	XM_010824413.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48662	XM_010801466.2 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48663	XM_015472924.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa48663	XM_015462101.1 Latest version!	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

PLCH1 ( XM_024995857.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851625.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa219229
Clone ID Related Accession (Same CDS sequence)	XM_024995857.1 , XM_024995849.1 , XM_024995851.1
Accession Version	XM_024995857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851625.1
CDS	174..5219
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_024995857.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995857.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_024995849.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851617.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OBa219229
Clone ID Related Accession (Same CDS sequence)	XM_024995857.1 , XM_024995849.1 , XM_024995851.1
Accession Version	XM_024995849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851617.1
CDS	1047..6092
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_024995849.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995849.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_024995851.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851619.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OBa219229
Clone ID Related Accession (Same CDS sequence)	XM_024995857.1 , XM_024995849.1 , XM_024995851.1
Accession Version	XM_024995851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851619.1
CDS	4850..9895
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_024995851.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995851.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_024995863.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851631.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa219230
Clone ID Related Accession (Same CDS sequence)	XM_024995863.1
Accession Version	XM_024995863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851631.1
CDS	1048..6090
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_024995863.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995863.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472917.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328403.2 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OBa219231
Clone ID Related Accession (Same CDS sequence)	XM_015472917.2
Accession Version	XM_015472917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328403.2
CDS	1049..6154
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472917.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472917.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGAGAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010801464.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799766.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010801464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799766.1
CDS	661..5766
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010801464.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801464.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010824408.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822710.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010824408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822710.1
CDS	825..5930
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010824408.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824408.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015462097.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317583.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015462097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317583.1
CDS	505..5610
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015462097.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462097.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010801461.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799763.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010801461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799763.1
CDS	825..5930
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010801461.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801461.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010801462.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799764.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010801462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799764.1
CDS	1021..6126
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010801462.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801462.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472917.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328403.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015472917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328403.1
CDS	270..5375
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472917.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472917.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472919.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328405.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015472919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328405.1
CDS	266..5371
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472919.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472919.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010824409.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822711.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010824409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822711.1
CDS	1021..6126
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010824409.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824409.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010824411.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822713.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_010824411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822713.1
CDS	661..5766
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010824411.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824411.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472914.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328400.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015472914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328400.1
CDS	505..5610
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472914.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472914.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015462098.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317584.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015462098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317584.1
CDS	270..5375
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015462098.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462098.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015462099.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317585.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa02642
Clone ID Related Accession (Same CDS sequence)	XM_010801464.2 , XM_010824408.2 , XM_015462097.1 , XM_010801461.2 , XM_010801462.2 , XM_015472917.1 , XM_015472919.1 , XM_010824409.2 , XM_010824411.2 , XM_015472914.1 , XM_015462098.1 , XM_015462099.1
Accession Version	XM_015462099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317585.1
CDS	266..5371
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015462099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462099.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472920.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328406.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa48661
Clone ID Related Accession (Same CDS sequence)	XM_015472920.1 , XM_015462100.1
Accession Version	XM_015472920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328406.1
CDS	946..6048
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472920.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472920.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015462100.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317586.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OBa48661
Clone ID Related Accession (Same CDS sequence)	XM_015472920.1 , XM_015462100.1
Accession Version	XM_015462100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317586.1
CDS	946..6048
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015462100.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462100.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTACA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGAAGATA AGGATGCCAG GAGAGATGGG 
AAGGCAAGTG TAAAAGACCA GCATCTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCC 
AGCGCTCTCA TCCACAAAGA CATCAGCCAA GGGAACTCTG CTGTTTCTAC TGCCAACTTG 
TCAATCACAG AACAGTTAGT GCCAGGTCCA AAGGGTGGGA GAACCAAATC AAACATGTTA 
AGTGATTGCT GGGAACATCA GTGCCTCAGC AGATCCCTCT CTCCAAGGCA GCATTTGGCT 
CATGATCCTG CAGTTAGCCC ATCAAAAGAT CTATGTGGTG TGAAAACCAA GGAAAATGGG 
AATGCTGGGG GCTTCGTGGT AGGGAAAAGC ACATTGTCAG GAAGCATCCT TTCTCAAAGC 
AATCTGGAGA TTAAGAAGCT GGAAGGTAAC TGGGATAAGG GCAGAGCTGC AACATCCTTT 
TCTTTGTCAG ACGTCTCTAC ACTCTGTTCT GATGCACCAG ACTTACATTC CACTGCAATT 
CTCCAGGAGA GTGAAATTTC CCACCTTATT GACAATGTCA CTTTAACAAA TGAGAATGAA 
CCTGGCAGTT CCATCTCAGC ACTGATTGGC CAGTTTGATG AGACCGGCAA CCAGGCTAAC 
CCTACAGTTG TTTCTCATCT TCAGAGCCCC AGTGTGATGT CGGGCCATCC TCCTGTGCCC 
ACCATGGACC TAAAAATGCC CTTCAAGCCT GGCTTTTCCA ATGGAAAACC CAAGTCATCC 
TTCCTGTGCT CGTCTCCTGA GCAGATTGCA CTCTCTGGAC ACGAGACCTG TGAATGTTCA 
ACACACACAG CTGTTGGTGA AACTATGTGC ACTCCTGTCT CTAAAATGAA ACCAGACAAT 
GACCTTTCTG GGAAGGCCAT GACTGGGGCC ATAGAAAACA ACCTGCCTCA GTCCTCTAAT 
ACTTCTCACT GCTGGTTACC AGAAAGTCCC ACCCATGGGA AAGACTGGGA AATCCTGAAG 
AACCCCAGCC CTGCCACTTC CACTGACTTG ACACTGGAGG ATGTAATAGC TGCTCCTACT 
CTCAGTTTAA ATTCTGGGGA GAGCAGTCTC GTAGAAATGG ATGGAGACTC AGAAAATCTG 
TCCCTAACAA CCTATGAATA TAGGAGAGAG GGCACAAGTC ACCTCACTTC TCCTTTAAAA 
GTGAAATACA GTCCAGCTGC AGTGGAACAT TTTCAAAGAG GCTTGAGAAA TGGCTACTGT 
AAAGAGACTC TCCACCCTTC TGTCTCTGAA ATATTCAACA ATACTCAAGA TGTCAAAAAT 
CAAAATATCT CTCATCTAGC CTATCAGGGT GCTGGGTTTA TGCATAATCA CTTCTCAAAT 
TCAGATGCAA AAACGAACCA GACCTCTGGG CCCCTGCCTT GTGCTCATGA CTTGCATGCC 
CCTGCCCCGG AGCAGTCCAC ACACTCTGCT CTGAAGCTGT CCAGTCCGTG CAAATCCAAA 
AGTCTTGGGG ACTTAACATC AGAGGACATC GCCTGTAATT TTGAAAGCAA GTACCAGTGT 
ATTAGTAAGA GCTTCGTTAC CACTGGCATC AGAGACAAGA AGGGCATGAC TGTAAAAACA 
AAGTCGTTAG AGCCTATGGA TGCCCTGACC GAGCAGCTGC GGAAACTTGT GTCCTTTGAC 
CAGGAAGATG GCTGCCAAGT GCTGTACTCC AAGCAAGATG CCAATCAATT CCCCAGGGCC 
TTGGTCAGAA AGTTGTCATC CAGAAGTCAG AGCAGAGTGC GCAATATTGC TAGTCGTGCC 
AAGGAGAAGC AGGAAGCCAA CAGGCAGAAG AGTGTGAACC CCAGCACTGT GGGAGGAGTG 
GTTCTTCGAA GCAAGCCGTG CGCGCCGGCC CCCACAGGGA ACAGGCACTC CACCGGTTCC 
TACATCGCGG GCTACCTGCG GAGCGCCAAG GGCGGCAGCT TGGAGGGCCG CGGCATCCCC 
GAGGGCGCAT GCGCAGCCCT GCGCTCCGGC CACGTCGACA GGTTCTGTTC ACATCATTCT 
GTTCTGCAGA CCGAACCCAG CAGTGATGAT AAACCAGAAA TTTATTTTCT TTTGAGACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010824413.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822715.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OBa48662
Clone ID Related Accession (Same CDS sequence)	XM_010824413.2 , XM_010801466.2
Accession Version	XM_010824413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGC 
GTAATGCAGT CTGGGACACA GATGATCAAG CTGAAGCGTG GGACCAAGGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA CCGGACCCGC CTCCGATGGA GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AGGGTCGGCA GTCTGAGATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCACG CACCTGGATT 
ACAGGCCTCA AGTACCTCAT GGCTGGCATC AGTGATGAAG ATTCCCTTGC CAAAAGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGCGATGGC 
TTATTGAACA TTGAAGAGAT ACACCAACTG ATGCACAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTCTGACT 
TTTGAAGAAT TCTGTGTGTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATCTGTTG 
CTTTTGAGCT ACAGTGACAA GAAAGATCAT CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAC AAATGTGACA ACAGACTATT GTATTGACAT CATAAGGAAA 
TTTGAAGTTT CTGAAGAAAA TAAGGTGAAA AATGTCCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCTTG TGACATCTTT AACCCCCTGC ATCATGAAGT GTACCAGGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCCTCCC ACAACACATA CCTGACTGGG 
GACCAGCTCC TGTCTCAGTC CAAAGTGGAC ATGTATGCGC GGGTGCTGCA GGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCGGACGGAC AGCCAGTGGT GCACCACGGT 
TACACGCTCA CTTCAAAGAT CCTCTTCAGA GATGCCGCGC ACCCCATCAA CAAGCATGCC 
TTTGTGAAGA ACGAGTTTCC AGTCATCCTG TCCATTGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAAA TTGCTCAGTA CCTGAAAGGA ATATTCCAAG ACAAACTCGA CCTGTCATCT 
ATAGACACAG GAGAGACCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTACCACCTT GGAGATGATG CTGAAGAAGG AGAAGTTTCT 
GATGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC GCTGTTAAAA 
GAATCTCAAA TTCGAGACAA GGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTCCTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGCATC CAGATGCAAA GGAAAGTGGA 
AAGAAAGCAC ATGGACGATC CCTCATGACC AACTTCGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AATCTTATAG TACTGATGAT GAGGAGGACG CACAGCAGAA TCCTGGCAAG 
GAAACTGGCC AGCTGTACCG GTTGGGCCGC CGAAGGAAAA CCATGAAGCT GTGTCGAGAG 
CTCTCTGATT TGGTTGTGTA CACAAACTCT GTGGCAGCCC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC CTTCAGTGAA ACAAGAGCAC ATCAAGTGGT TCAGCAGAAA 
TCAGAGCAGT TCATGATTTA TAACCAAAAG CAGCTCACGA GGATTTATCC CTCCGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCTCTG CCCTACTGGA ACGCAGGTTG CCAGCTAGTG 
GCACTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGTTAA ATCGAGCCAA ATTCAAGACA 
AACGGCAATT GCGGCTACGT TCTCAAACCC CAGCAGATGT GCAAAGGTAC CTTCAACCCT 
TTCTCAGGTG ATCCACTTCC TGCCAACCCC AAAAAACAGC TCATCCTGAA AGTGATCAGT 
GGACAACAAC TCCCCAAACC TCCAGATTCC ATGTTTGGAG ACCGAGGAGA GATCATCGAT 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTCTAAAGA TCAAACTCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTA TGGGAAGAAA CCCTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT CCGGTTCCTC GTGTGGGATC ATGATCCAAT TGGACGAGAC 
TTTGTTGGGC AAAGAACTGT GACCTTCAGC AGCTTGGTGC CTGGCTACCG GCATGTCTAC 
TTGGAAGGAC TGACAGAAGC ATCTATATTT GTCCACATAA CAATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TACACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG TTTGAAGGGG CTGTTCAATA AGAATCCCAG ACACAATTCT 
TCAGAAAACA CTTGCCATTA CATTCGGAAG CGATCGATTG GAGATAAGAT TCTGCGACGC 
ACAGCCAGCG CTCCGGCCAA AGGCAGAAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTA 
GAGATGAAGG ATTCTGTTTC TGAGGCTGCA AGAGATCAAG ATGGGGTCCT GAGAAGGACC 
ACGCGGAGTT TGCAAGCGCG CCCTGTCTCT ATGCCTGTTG ACAGAAGTCT TCTTGGGGCT 
CTGTCGCTGC CTGTATCTGA AACCACAAAA GACACTGAAG GAAAGGAAAA CTCTCAAGCA 
GAAGATAAGG ATGCCAGGAG AGATGGGAAG GCAAGTGTAA AAGACCAGCA TCTTCCAAAT 
TTCAACAAAA AGTTATCCTC TTCCTCCAGC GCTCTCATCC ACAAAGACAT CAGCCAAGGG 
AACTCTGCTG TTTCTACTGC CAACTTGTCA ATCACAGAAC AGTTAGTGCC AGGTCCAAAG 
GGTGGGAGAA CCAAATCAAA CATGTTAAGT GATTGCTGGG AACATCAGTG CCTCAGCAGA 
TCCCTCTCTC CAAGGCAGCA TTTGGCTCAT GATCCTGCAG TTAGCCCATC AAAAGATCTA 
TGTGGTGTGA AAACCAAGGA AAATGGGAAT GCTGGGGGCT TCGTGGTAGG GAAAAGCACA 
TTGTCAGGAA GCATCCTTTC TCAAAGCAAT CTGGAGATTA AGAAGCTGGA AGGTAACTGG 
GATAAGGGCA GAGCTGCAAC ATCCTTTTCT TTGTCAGACG TCTCTACACT CTGTTCTGAT 
GCACCAGACT TACATTCCAC TGCAATTCTC CAGGAGAGTG AAATTTCCCA CCTTATTGAC 
AATGTCACTT TAACAAATGA GAATGAACCT GGCAGTTCCA TCTCAGCACT GATTGGCCAG 
TTTGATGAGA CCGGCAACCA GGCTAACCCT ACAGTTGTTT CTCATCTTCA GAGCCCCAGT 
GTGATGTCGG GCCATCCTCC TGTGCCCACC ATGGACCTAA AAATGCCCTT CAAGCCTGGC 
TTTTCCAATG GAAAACCCAA GTCATCCTTC CTGTGCTCGT CTCCTGAGCA GATTGCACTC 
TCTGGACACG AGACCTGTGA ATGTTCAACA CACACAGCTG TTGGTGAAAC TATGTGCACT 
CCTGTCTCTA AAATGAAACC AGACAATGAC CTTTCTGGGA AGGCCATGAC TGGGGCCATA 
GAAAACAACC TGCCTCAGTC CTCTAATACT TCTCACTGCT GGTTACCAGA AAGTCCCACC 
CATGGGAAAG ACTGGGAAAT CCTGAAGAAC CCCAGCCCTG CCACTTCCAC TGACTTGACA 
CTGGAGGATG TAATAGCTGC TCCTACTCTC AGTTTAAATT CTGGGGAGAG CAGTCTCGTA 
GAAATGGATG GAGACTCAGA AAATCTGTCC CTAACAACCT ATGAATATAG GAGAGAGGGC 
ACAAGTCACC TCACTTCTCC TTTAAAAGTG AAATACAGTC CAGCTGCAGT GGAACATTTT 
CAAAGAGGCT TGAGAAATGG CTACTGTAAA GAGACTCTCC ACCCTTCTGT CTCTGAAATA 
TTCAACAATA CTCAAGATGT CAAAAATCAA AATATCTCTC ATCTAGCCTA TCAGGGTGCT 
GGGTTTATGC ATAATCACTT CTCAAATTCA GATGCAAAAA CGAACCAGAC CTCTGGGCCC 
CTGCCTTGTG CTCATGACTT GCATGCCCCT GCCCCGGAGC AGTCCACACA CTCTGCTCTG 
AAGCTGTCCA GTCCGTGCAA ATCCAAAAGT CTTGGGGACT TAACATCAGA GGACATCGCC 
TGTAATTTTG AAAGCAAGTA CCAGTGTATT AGTAAGAGCT TCGTTACCAC TGGCATCAGA 
GACAAGAAGG GCATGACTGT AAAAACAAAG TCGTTAGAGC CTATGGATGC CCTGACCGAG 
CAGCTGCGGA AACTTGTGTC CTTTGACCAG GAAGATGGCT GCCAAGTGCT GTACTCCAAG 
CAAGATGCCA ATCAATTCCC CAGGGCCTTG GTCAGAAAGT TGTCATCCAG AAGTCAGAGC 
AGAGTGCGCA ATATTGCTAG TCGTGCCAAG GAGAAGCAGG AAGCCAACAG GCAGAAGAGT 
GTGAACCCCA GCACTGTGGG AGGAGTGGTT CTTCGAAGCA AGCCGTGCGC GCCGGCCCCC 
ACAGGGAACA GGCACTCCAC CGGTTCCTAC ATCGCGGGCT ACCTGCGGAG CGCCAAGGGC 
GGCAGCTTGG AGGGCCGCGG CATCCCCGAG GGCGCATGCG CAGCCCTGCG CTCCGGCCAC 
GTCGACAGGT TCTGTTCACA TCATTCTGTT CTGCAGACCG AACCCAGCAG TGATGATAAA 
CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822715.1
CDS	1180..6249
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010824413.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824413.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGC 
GTAATGCAGT CTGGGACACA GATGATCAAG CTGAAGCGTG GGACCAAGGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA CCGGACCCGC CTCCGATGGA GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AGGGTCGGCA GTCTGAGATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCACG CACCTGGATT 
ACAGGCCTCA AGTACCTCAT GGCTGGCATC AGTGATGAAG ATTCCCTTGC CAAAAGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGCGATGGC 
TTATTGAACA TTGAAGAGAT ACACCAACTG ATGCACAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTCTGACT 
TTTGAAGAAT TCTGTGTGTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATCTGTTG 
CTTTTGAGCT ACAGTGACAA GAAAGATCAT CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAC AAATGTGACA ACAGACTATT GTATTGACAT CATAAGGAAA 
TTTGAAGTTT CTGAAGAAAA TAAGGTGAAA AATGTCCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCTTG TGACATCTTT AACCCCCTGC ATCATGAAGT GTACCAGGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCCTCCC ACAACACATA CCTGACTGGG 
GACCAGCTCC TGTCTCAGTC CAAAGTGGAC ATGTATGCGC GGGTGCTGCA GGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCGGACGGAC AGCCAGTGGT GCACCACGGT 
TACACGCTCA CTTCAAAGAT CCTCTTCAGA GATGCCGCGC ACCCCATCAA CAAGCATGCC 
TTTGTGAAGA ACGAGTTTCC AGTCATCCTG TCCATTGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAAA TTGCTCAGTA CCTGAAAGGA ATATTCCAAG ACAAACTCGA CCTGTCATCT 
ATAGACACAG GAGAGACCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTACCACCTT GGAGATGATG CTGAAGAAGG AGAAGTTTCT 
GATGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC GCTGTTAAAA 
GAATCTCAAA TTCGAGACAA GGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTCCTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGCATC CAGATGCAAA GGAAAGTGGA 
AAGAAAGCAC ATGGACGATC CCTCATGACC AACTTCGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AATCTTATAG TACTGATGAT GAGGAGGACG CACAGCAGAA TCCTGGCAAG 
GAAACTGGCC AGCTGTACCG GTTGGGCCGC CGAAGGAAAA CCATGAAGCT GTGTCGAGAG 
CTCTCTGATT TGGTTGTGTA CACAAACTCT GTGGCAGCCC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC CTTCAGTGAA ACAAGAGCAC ATCAAGTGGT TCAGCAGAAA 
TCAGAGCAGT TCATGATTTA TAACCAAAAG CAGCTCACGA GGATTTATCC CTCCGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCTCTG CCCTACTGGA ACGCAGGTTG CCAGCTAGTG 
GCACTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGTTAA ATCGAGCCAA ATTCAAGACA 
AACGGCAATT GCGGCTACGT TCTCAAACCC CAGCAGATGT GCAAAGGTAC CTTCAACCCT 
TTCTCAGGTG ATCCACTTCC TGCCAACCCC AAAAAACAGC TCATCCTGAA AGTGATCAGT 
GGACAACAAC TCCCCAAACC TCCAGATTCC ATGTTTGGAG ACCGAGGAGA GATCATCGAT 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTCTAAAGA TCAAACTCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTA TGGGAAGAAA CCCTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT CCGGTTCCTC GTGTGGGATC ATGATCCAAT TGGACGAGAC 
TTTGTTGGGC AAAGAACTGT GACCTTCAGC AGCTTGGTGC CTGGCTACCG GCATGTCTAC 
TTGGAAGGAC TGACAGAAGC ATCTATATTT GTCCACATAA CAATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TACACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG TTTGAAGGGG CTGTTCAATA AGAATCCCAG ACACAATTCT 
TCAGAAAACA CTTGCCATTA CATTCGGAAG CGATCGATTG GAGATAAGAT TCTGCGACGC 
ACAGCCAGCG CTCCGGCCAA AGGCAGAAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTA 
GAGATGAAGG ATTCTGTTTC TGAGGCTGCA AGAGATCAAG ATGGGGTCCT GAGAAGGACC 
ACGCGGAGTT TGCAAGCGCG CCCTGTCTCT ATGCCTGTTG ACAGAAGTCT TCTTGGGGCT 
CTGTCGCTGC CTGTATCTGA AACCACAAAA GACACTGAAG GAAAGGAAAA CTCTCAAGCA 
GAAGATAAGG ATGCCAGGAG AGATGGGAAG GCAAGTGTAA AAGACCAGCA TCTTCCAAAT 
TTCAACAAAA AGTTATCCTC TTCCTCCAGC GCTCTCATCC ACAAAGACAT CAGCCAAGGG 
AACTCTGCTG TTTCTACTGC CAACTTGTCA ATCACAGAAC AGTTAGTGCC AGGTCCAAAG 
GGTGGGAGAA CCAAATCAAA CATGTTAAGT GATTGCTGGG AACATCAGTG CCTCAGCAGA 
TCCCTCTCTC CAAGGCAGCA TTTGGCTCAT GATCCTGCAG TTAGCCCATC AAAAGATCTA 
TGTGGTGTGA AAACCAAGGA AAATGGGAAT GCTGGGGGCT TCGTGGTAGG GAAAAGCACA 
TTGTCAGGAA GCATCCTTTC TCAAAGCAAT CTGGAGATTA AGAAGCTGGA AGGTAACTGG 
GATAAGGGCA GAGCTGCAAC ATCCTTTTCT TTGTCAGACG TCTCTACACT CTGTTCTGAT 
GCACCAGACT TACATTCCAC TGCAATTCTC CAGGAGAGTG AAATTTCCCA CCTTATTGAC 
AATGTCACTT TAACAAATGA GAATGAACCT GGCAGTTCCA TCTCAGCACT GATTGGCCAG 
TTTGATGAGA CCGGCAACCA GGCTAACCCT ACAGTTGTTT CTCATCTTCA GAGCCCCAGT 
GTGATGTCGG GCCATCCTCC TGTGCCCACC ATGGACCTAA AAATGCCCTT CAAGCCTGGC 
TTTTCCAATG GAAAACCCAA GTCATCCTTC CTGTGCTCGT CTCCTGAGCA GATTGCACTC 
TCTGGACACG AGACCTGTGA ATGTTCAACA CACACAGCTG TTGGTGAAAC TATGTGCACT 
CCTGTCTCTA AAATGAAACC AGACAATGAC CTTTCTGGGA AGGCCATGAC TGGGGCCATA 
GAAAACAACC TGCCTCAGTC CTCTAATACT TCTCACTGCT GGTTACCAGA AAGTCCCACC 
CATGGGAAAG ACTGGGAAAT CCTGAAGAAC CCCAGCCCTG CCACTTCCAC TGACTTGACA 
CTGGAGGATG TAATAGCTGC TCCTACTCTC AGTTTAAATT CTGGGGAGAG CAGTCTCGTA 
GAAATGGATG GAGACTCAGA AAATCTGTCC CTAACAACCT ATGAATATAG GAGAGAGGGC 
ACAAGTCACC TCACTTCTCC TTTAAAAGTG AAATACAGTC CAGCTGCAGT GGAACATTTT 
CAAAGAGGCT TGAGAAATGG CTACTGTAAA GAGACTCTCC ACCCTTCTGT CTCTGAAATA 
TTCAACAATA CTCAAGATGT CAAAAATCAA AATATCTCTC ATCTAGCCTA TCAGGGTGCT 
GGGTTTATGC ATAATCACTT CTCAAATTCA GATGCAAAAA CGAACCAGAC CTCTGGGCCC 
CTGCCTTGTG CTCATGACTT GCATGCCCCT GCCCCGGAGC AGTCCACACA CTCTGCTCTG 
AAGCTGTCCA GTCCGTGCAA ATCCAAAAGT CTTGGGGACT TAACATCAGA GGACATCGCC 
TGTAATTTTG AAAGCAAGTA CCAGTGTATT AGTAAGAGCT TCGTTACCAC TGGCATCAGA 
GACAAGAAGG GCATGACTGT AAAAACAAAG TCGTTAGAGC CTATGGATGC CCTGACCGAG 
CAGCTGCGGA AACTTGTGTC CTTTGACCAG GAAGATGGCT GCCAAGTGCT GTACTCCAAG 
CAAGATGCCA ATCAATTCCC CAGGGCCTTG GTCAGAAAGT TGTCATCCAG AAGTCAGAGC 
AGAGTGCGCA ATATTGCTAG TCGTGCCAAG GAGAAGCAGG AAGCCAACAG GCAGAAGAGT 
GTGAACCCCA GCACTGTGGG AGGAGTGGTT CTTCGAAGCA AGCCGTGCGC GCCGGCCCCC 
ACAGGGAACA GGCACTCCAC CGGTTCCTAC ATCGCGGGCT ACCTGCGGAG CGCCAAGGGC 
GGCAGCTTGG AGGGCCGCGG CATCCCCGAG GGCGCATGCG CAGCCCTGCG CTCCGGCCAC 
GTCGACAGGT TCTGTTCACA TCATTCTGTT CTGCAGACCG AACCCAGCAG TGATGATAAA 
CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_010801466.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799768.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OBa48662
Clone ID Related Accession (Same CDS sequence)	XM_010824413.2 , XM_010801466.2
Accession Version	XM_010801466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGC 
GTAATGCAGT CTGGGACACA GATGATCAAG CTGAAGCGTG GGACCAAGGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA CCGGACCCGC CTCCGATGGA GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AGGGTCGGCA GTCTGAGATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCACG CACCTGGATT 
ACAGGCCTCA AGTACCTCAT GGCTGGCATC AGTGATGAAG ATTCCCTTGC CAAAAGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGCGATGGC 
TTATTGAACA TTGAAGAGAT ACACCAACTG ATGCACAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTCTGACT 
TTTGAAGAAT TCTGTGTGTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATCTGTTG 
CTTTTGAGCT ACAGTGACAA GAAAGATCAT CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAC AAATGTGACA ACAGACTATT GTATTGACAT CATAAGGAAA 
TTTGAAGTTT CTGAAGAAAA TAAGGTGAAA AATGTCCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCTTG TGACATCTTT AACCCCCTGC ATCATGAAGT GTACCAGGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCCTCCC ACAACACATA CCTGACTGGG 
GACCAGCTCC TGTCTCAGTC CAAAGTGGAC ATGTATGCGC GGGTGCTGCA GGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCGGACGGAC AGCCAGTGGT GCACCACGGT 
TACACGCTCA CTTCAAAGAT CCTCTTCAGA GATGCCGCGC ACCCCATCAA CAAGCATGCC 
TTTGTGAAGA ACGAGTTTCC AGTCATCCTG TCCATTGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAAA TTGCTCAGTA CCTGAAAGGA ATATTCCAAG ACAAACTCGA CCTGTCATCT 
ATAGACACAG GAGAGACCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTACCACCTT GGAGATGATG CTGAAGAAGG AGAAGTTTCT 
GATGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC GCTGTTAAAA 
GAATCTCAAA TTCGAGACAA GGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTCCTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGCATC CAGATGCAAA GGAAAGTGGA 
AAGAAAGCAC ATGGACGATC CCTCATGACC AACTTCGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AATCTTATAG TACTGATGAT GAGGAGGACG CACAGCAGAA TCCTGGCAAG 
GAAACTGGCC AGCTGTACCG GTTGGGCCGC CGAAGGAAAA CCATGAAGCT GTGTCGAGAG 
CTCTCTGATT TGGTTGTGTA CACAAACTCT GTGGCAGCCC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC CTTCAGTGAA ACAAGAGCAC ATCAAGTGGT TCAGCAGAAA 
TCAGAGCAGT TCATGATTTA TAACCAAAAG CAGCTCACGA GGATTTATCC CTCCGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCTCTG CCCTACTGGA ACGCAGGTTG CCAGCTAGTG 
GCACTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGTTAA ATCGAGCCAA ATTCAAGACA 
AACGGCAATT GCGGCTACGT TCTCAAACCC CAGCAGATGT GCAAAGGTAC CTTCAACCCT 
TTCTCAGGTG ATCCACTTCC TGCCAACCCC AAAAAACAGC TCATCCTGAA AGTGATCAGT 
GGACAACAAC TCCCCAAACC TCCAGATTCC ATGTTTGGAG ACCGAGGAGA GATCATCGAT 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTCTAAAGA TCAAACTCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTA TGGGAAGAAA CCCTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT CCGGTTCCTC GTGTGGGATC ATGATCCAAT TGGACGAGAC 
TTTGTTGGGC AAAGAACTGT GACCTTCAGC AGCTTGGTGC CTGGCTACCG GCATGTCTAC 
TTGGAAGGAC TGACAGAAGC ATCTATATTT GTCCACATAA CAATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TACACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG TTTGAAGGGG CTGTTCAATA AGAATCCCAG ACACAATTCT 
TCAGAAAACA CTTGCCATTA CATTCGGAAG CGATCGATTG GAGATAAGAT TCTGCGACGC 
ACAGCCAGCG CTCCGGCCAA AGGCAGAAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTA 
GAGATGAAGG ATTCTGTTTC TGAGGCTGCA AGAGATCAAG ATGGGGTCCT GAGAAGGACC 
ACGCGGAGTT TGCAAGCGCG CCCTGTCTCT ATGCCTGTTG ACAGAAGTCT TCTTGGGGCT 
CTGTCGCTGC CTGTATCTGA AACCACAAAA GACACTGAAG GAAAGGAAAA CTCTCAAGCA 
GAAGATAAGG ATGCCAGGAG AGATGGGAAG GCAAGTGTAA AAGACCAGCA TCTTCCAAAT 
TTCAACAAAA AGTTATCCTC TTCCTCCAGC GCTCTCATCC ACAAAGACAT CAGCCAAGGG 
AACTCTGCTG TTTCTACTGC CAACTTGTCA ATCACAGAAC AGTTAGTGCC AGGTCCAAAG 
GGTGGGAGAA CCAAATCAAA CATGTTAAGT GATTGCTGGG AACATCAGTG CCTCAGCAGA 
TCCCTCTCTC CAAGGCAGCA TTTGGCTCAT GATCCTGCAG TTAGCCCATC AAAAGATCTA 
TGTGGTGTGA AAACCAAGGA AAATGGGAAT GCTGGGGGCT TCGTGGTAGG GAAAAGCACA 
TTGTCAGGAA GCATCCTTTC TCAAAGCAAT CTGGAGATTA AGAAGCTGGA AGGTAACTGG 
GATAAGGGCA GAGCTGCAAC ATCCTTTTCT TTGTCAGACG TCTCTACACT CTGTTCTGAT 
GCACCAGACT TACATTCCAC TGCAATTCTC CAGGAGAGTG AAATTTCCCA CCTTATTGAC 
AATGTCACTT TAACAAATGA GAATGAACCT GGCAGTTCCA TCTCAGCACT GATTGGCCAG 
TTTGATGAGA CCGGCAACCA GGCTAACCCT ACAGTTGTTT CTCATCTTCA GAGCCCCAGT 
GTGATGTCGG GCCATCCTCC TGTGCCCACC ATGGACCTAA AAATGCCCTT CAAGCCTGGC 
TTTTCCAATG GAAAACCCAA GTCATCCTTC CTGTGCTCGT CTCCTGAGCA GATTGCACTC 
TCTGGACACG AGACCTGTGA ATGTTCAACA CACACAGCTG TTGGTGAAAC TATGTGCACT 
CCTGTCTCTA AAATGAAACC AGACAATGAC CTTTCTGGGA AGGCCATGAC TGGGGCCATA 
GAAAACAACC TGCCTCAGTC CTCTAATACT TCTCACTGCT GGTTACCAGA AAGTCCCACC 
CATGGGAAAG ACTGGGAAAT CCTGAAGAAC CCCAGCCCTG CCACTTCCAC TGACTTGACA 
CTGGAGGATG TAATAGCTGC TCCTACTCTC AGTTTAAATT CTGGGGAGAG CAGTCTCGTA 
GAAATGGATG GAGACTCAGA AAATCTGTCC CTAACAACCT ATGAATATAG GAGAGAGGGC 
ACAAGTCACC TCACTTCTCC TTTAAAAGTG AAATACAGTC CAGCTGCAGT GGAACATTTT 
CAAAGAGGCT TGAGAAATGG CTACTGTAAA GAGACTCTCC ACCCTTCTGT CTCTGAAATA 
TTCAACAATA CTCAAGATGT CAAAAATCAA AATATCTCTC ATCTAGCCTA TCAGGGTGCT 
GGGTTTATGC ATAATCACTT CTCAAATTCA GATGCAAAAA CGAACCAGAC CTCTGGGCCC 
CTGCCTTGTG CTCATGACTT GCATGCCCCT GCCCCGGAGC AGTCCACACA CTCTGCTCTG 
AAGCTGTCCA GTCCGTGCAA ATCCAAAAGT CTTGGGGACT TAACATCAGA GGACATCGCC 
TGTAATTTTG AAAGCAAGTA CCAGTGTATT AGTAAGAGCT TCGTTACCAC TGGCATCAGA 
GACAAGAAGG GCATGACTGT AAAAACAAAG TCGTTAGAGC CTATGGATGC CCTGACCGAG 
CAGCTGCGGA AACTTGTGTC CTTTGACCAG GAAGATGGCT GCCAAGTGCT GTACTCCAAG 
CAAGATGCCA ATCAATTCCC CAGGGCCTTG GTCAGAAAGT TGTCATCCAG AAGTCAGAGC 
AGAGTGCGCA ATATTGCTAG TCGTGCCAAG GAGAAGCAGG AAGCCAACAG GCAGAAGAGT 
GTGAACCCCA GCACTGTGGG AGGAGTGGTT CTTCGAAGCA AGCCGTGCGC GCCGGCCCCC 
ACAGGGAACA GGCACTCCAC CGGTTCCTAC ATCGCGGGCT ACCTGCGGAG CGCCAAGGGC 
GGCAGCTTGG AGGGCCGCGG CATCCCCGAG GGCGCATGCG CAGCCCTGCG CTCCGGCCAC 
GTCGACAGGT TCTGTTCACA TCATTCTGTT CTGCAGACCG AACCCAGCAG TGATGATAAA 
CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799768.1
CDS	1180..6249
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_010801466.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801466.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGC 
GTAATGCAGT CTGGGACACA GATGATCAAG CTGAAGCGTG GGACCAAGGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA CCGGACCCGC CTCCGATGGA GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AGGGTCGGCA GTCTGAGATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCACG CACCTGGATT 
ACAGGCCTCA AGTACCTCAT GGCTGGCATC AGTGATGAAG ATTCCCTTGC CAAAAGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGCGATGGC 
TTATTGAACA TTGAAGAGAT ACACCAACTG ATGCACAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTCTGACT 
TTTGAAGAAT TCTGTGTGTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATCTGTTG 
CTTTTGAGCT ACAGTGACAA GAAAGATCAT CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAC AAATGTGACA ACAGACTATT GTATTGACAT CATAAGGAAA 
TTTGAAGTTT CTGAAGAAAA TAAGGTGAAA AATGTCCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCTTG TGACATCTTT AACCCCCTGC ATCATGAAGT GTACCAGGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCCTCCC ACAACACATA CCTGACTGGG 
GACCAGCTCC TGTCTCAGTC CAAAGTGGAC ATGTATGCGC GGGTGCTGCA GGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCGGACGGAC AGCCAGTGGT GCACCACGGT 
TACACGCTCA CTTCAAAGAT CCTCTTCAGA GATGCCGCGC ACCCCATCAA CAAGCATGCC 
TTTGTGAAGA ACGAGTTTCC AGTCATCCTG TCCATTGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAAA TTGCTCAGTA CCTGAAAGGA ATATTCCAAG ACAAACTCGA CCTGTCATCT 
ATAGACACAG GAGAGACCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTACCACCTT GGAGATGATG CTGAAGAAGG AGAAGTTTCT 
GATGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC GCTGTTAAAA 
GAATCTCAAA TTCGAGACAA GGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTCCTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGCATC CAGATGCAAA GGAAAGTGGA 
AAGAAAGCAC ATGGACGATC CCTCATGACC AACTTCGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AATCTTATAG TACTGATGAT GAGGAGGACG CACAGCAGAA TCCTGGCAAG 
GAAACTGGCC AGCTGTACCG GTTGGGCCGC CGAAGGAAAA CCATGAAGCT GTGTCGAGAG 
CTCTCTGATT TGGTTGTGTA CACAAACTCT GTGGCAGCCC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC CTTCAGTGAA ACAAGAGCAC ATCAAGTGGT TCAGCAGAAA 
TCAGAGCAGT TCATGATTTA TAACCAAAAG CAGCTCACGA GGATTTATCC CTCCGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCTCTG CCCTACTGGA ACGCAGGTTG CCAGCTAGTG 
GCACTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGTTAA ATCGAGCCAA ATTCAAGACA 
AACGGCAATT GCGGCTACGT TCTCAAACCC CAGCAGATGT GCAAAGGTAC CTTCAACCCT 
TTCTCAGGTG ATCCACTTCC TGCCAACCCC AAAAAACAGC TCATCCTGAA AGTGATCAGT 
GGACAACAAC TCCCCAAACC TCCAGATTCC ATGTTTGGAG ACCGAGGAGA GATCATCGAT 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTCTAAAGA TCAAACTCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTA TGGGAAGAAA CCCTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT CCGGTTCCTC GTGTGGGATC ATGATCCAAT TGGACGAGAC 
TTTGTTGGGC AAAGAACTGT GACCTTCAGC AGCTTGGTGC CTGGCTACCG GCATGTCTAC 
TTGGAAGGAC TGACAGAAGC ATCTATATTT GTCCACATAA CAATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TACACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG TTTGAAGGGG CTGTTCAATA AGAATCCCAG ACACAATTCT 
TCAGAAAACA CTTGCCATTA CATTCGGAAG CGATCGATTG GAGATAAGAT TCTGCGACGC 
ACAGCCAGCG CTCCGGCCAA AGGCAGAAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTA 
GAGATGAAGG ATTCTGTTTC TGAGGCTGCA AGAGATCAAG ATGGGGTCCT GAGAAGGACC 
ACGCGGAGTT TGCAAGCGCG CCCTGTCTCT ATGCCTGTTG ACAGAAGTCT TCTTGGGGCT 
CTGTCGCTGC CTGTATCTGA AACCACAAAA GACACTGAAG GAAAGGAAAA CTCTCAAGCA 
GAAGATAAGG ATGCCAGGAG AGATGGGAAG GCAAGTGTAA AAGACCAGCA TCTTCCAAAT 
TTCAACAAAA AGTTATCCTC TTCCTCCAGC GCTCTCATCC ACAAAGACAT CAGCCAAGGG 
AACTCTGCTG TTTCTACTGC CAACTTGTCA ATCACAGAAC AGTTAGTGCC AGGTCCAAAG 
GGTGGGAGAA CCAAATCAAA CATGTTAAGT GATTGCTGGG AACATCAGTG CCTCAGCAGA 
TCCCTCTCTC CAAGGCAGCA TTTGGCTCAT GATCCTGCAG TTAGCCCATC AAAAGATCTA 
TGTGGTGTGA AAACCAAGGA AAATGGGAAT GCTGGGGGCT TCGTGGTAGG GAAAAGCACA 
TTGTCAGGAA GCATCCTTTC TCAAAGCAAT CTGGAGATTA AGAAGCTGGA AGGTAACTGG 
GATAAGGGCA GAGCTGCAAC ATCCTTTTCT TTGTCAGACG TCTCTACACT CTGTTCTGAT 
GCACCAGACT TACATTCCAC TGCAATTCTC CAGGAGAGTG AAATTTCCCA CCTTATTGAC 
AATGTCACTT TAACAAATGA GAATGAACCT GGCAGTTCCA TCTCAGCACT GATTGGCCAG 
TTTGATGAGA CCGGCAACCA GGCTAACCCT ACAGTTGTTT CTCATCTTCA GAGCCCCAGT 
GTGATGTCGG GCCATCCTCC TGTGCCCACC ATGGACCTAA AAATGCCCTT CAAGCCTGGC 
TTTTCCAATG GAAAACCCAA GTCATCCTTC CTGTGCTCGT CTCCTGAGCA GATTGCACTC 
TCTGGACACG AGACCTGTGA ATGTTCAACA CACACAGCTG TTGGTGAAAC TATGTGCACT 
CCTGTCTCTA AAATGAAACC AGACAATGAC CTTTCTGGGA AGGCCATGAC TGGGGCCATA 
GAAAACAACC TGCCTCAGTC CTCTAATACT TCTCACTGCT GGTTACCAGA AAGTCCCACC 
CATGGGAAAG ACTGGGAAAT CCTGAAGAAC CCCAGCCCTG CCACTTCCAC TGACTTGACA 
CTGGAGGATG TAATAGCTGC TCCTACTCTC AGTTTAAATT CTGGGGAGAG CAGTCTCGTA 
GAAATGGATG GAGACTCAGA AAATCTGTCC CTAACAACCT ATGAATATAG GAGAGAGGGC 
ACAAGTCACC TCACTTCTCC TTTAAAAGTG AAATACAGTC CAGCTGCAGT GGAACATTTT 
CAAAGAGGCT TGAGAAATGG CTACTGTAAA GAGACTCTCC ACCCTTCTGT CTCTGAAATA 
TTCAACAATA CTCAAGATGT CAAAAATCAA AATATCTCTC ATCTAGCCTA TCAGGGTGCT 
GGGTTTATGC ATAATCACTT CTCAAATTCA GATGCAAAAA CGAACCAGAC CTCTGGGCCC 
CTGCCTTGTG CTCATGACTT GCATGCCCCT GCCCCGGAGC AGTCCACACA CTCTGCTCTG 
AAGCTGTCCA GTCCGTGCAA ATCCAAAAGT CTTGGGGACT TAACATCAGA GGACATCGCC 
TGTAATTTTG AAAGCAAGTA CCAGTGTATT AGTAAGAGCT TCGTTACCAC TGGCATCAGA 
GACAAGAAGG GCATGACTGT AAAAACAAAG TCGTTAGAGC CTATGGATGC CCTGACCGAG 
CAGCTGCGGA AACTTGTGTC CTTTGACCAG GAAGATGGCT GCCAAGTGCT GTACTCCAAG 
CAAGATGCCA ATCAATTCCC CAGGGCCTTG GTCAGAAAGT TGTCATCCAG AAGTCAGAGC 
AGAGTGCGCA ATATTGCTAG TCGTGCCAAG GAGAAGCAGG AAGCCAACAG GCAGAAGAGT 
GTGAACCCCA GCACTGTGGG AGGAGTGGTT CTTCGAAGCA AGCCGTGCGC GCCGGCCCCC 
ACAGGGAACA GGCACTCCAC CGGTTCCTAC ATCGCGGGCT ACCTGCGGAG CGCCAAGGGC 
GGCAGCTTGG AGGGCCGCGG CATCCCCGAG GGCGCATGCG CAGCCCTGCG CTCCGGCCAC 
GTCGACAGGT TCTGTTCACA TCATTCTGTT CTGCAGACCG AACCCAGCAG TGATGATAAA 
CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015472924.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328410.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OBa48663
Clone ID Related Accession (Same CDS sequence)	XM_015472924.1 , XM_015462101.1
Accession Version	XM_015472924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328410.1
CDS	945..5990
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015472924.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472924.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015462101.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317587.1 Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OBa48663
Clone ID Related Accession (Same CDS sequence)	XM_015472924.1 , XM_015462101.1
Accession Version	XM_015462101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317587.1
CDS	945..5990
Translation	MSYWNLEKRNCVQYRRHFLVDNSPFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMTNVTTDYCIDIIRKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGQPVVHHGYTLTSKILFRDAAHPINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFQDKLDLSSIDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDAKESGKKAHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDAQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCSKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHNSSENTCHYIRKRSIGDKILRRTASAPAKGRKKSKMGFQEMVEMKDSVSEAARDQDGVLRRTTRSLQARPVSMPVDRSLLGALSLPVSETTKDTEGKENSQAEDKDARRDGKASVKDQHLPNFNKKLSSSSSALIHKDISQGNSAVSTANLSITEQLVPGPKGGRTKSNMLSDCWEHQCLSRSLSPRQHLAHDPAVSPSKDLCGVKTKENGNAGGFVVGKSTLSGSILSQSNLEIKKLEGNWDKGRAATSFSLSDVSTLCSDAPDLHSTAILQESEISHLIDNVTLTNENEPGSSISALIGQFDETGNQANPTVVSHLQSPSVMSGHPPVPTMDLKMPFKPGFSNGKPKSSFLCSSPEQIALSGHETCECSTHTAVGETMCTPVSKMKPDNDLSGKAMTGAIENNLPQSSNTSHCWLPESPTHGKDWEILKNPSPATSTDLTLEDVIAAPTLSLNSGESSLVEMDGDSENLSLTTYEYRREGTSHLTSPLKVKYSPAAVEHFQRGLRNGYCKETLHPSVSEIFNNTQDVKNQNISHLAYQGAGFMHNHFSNSDAKTNQTSGPLPCAHDLHAPAPEQSTHSALKLSSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGMTVKTKSLEPMDALTEQLRKLVSFDQEDGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANRQKSVNPSTVGGVVLRSKPCAPAPTGNRHSTGSYIAGYLRSAKGGSLEGRGIPEGACAALRSGHVDRFCSHHSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_015462101.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462101.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA GAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTC CGTTTCATGT TGAAAGATGC ATGAGCGTAA TGCAGTCTGG GACACAGATG 
ATCAAGCTGA AGCGTGGGAC CAAGGGGCTC GTCCGGCTCT TTTACCTGGA TGAACACCGG 
ACCCGCCTCC GATGGAGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAGGG TCGGCAGTCT GAGATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCACGCACC TGGATTACAG GCCTCAAGTA CCTCATGGCT 
GGCATCAGTG ATGAAGATTC CCTTGCCAAA AGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGC GATGGCTTAT TGAACATTGA AGAGATACAC 
CAACTGATGC ACAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT CTGACTTTTG AAGAATTCTG TGTGTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT CTGTTGCTTT TGAGCTACAG TGACAAGAAA 
GATCATCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGACAAAT 
GTGACAACAG ACTATTGTAT TGACATCATA AGGAAATTTG AAGTTTCTGA AGAAAATAAG 
GTGAAAAATG TCCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCTTGTGAC 
ATCTTTAACC CCCTGCATCA TGAAGTGTAC CAGGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CCTCCCACAA CACATACCTG ACTGGGGACC AGCTCCTGTC TCAGTCCAAA 
GTGGACATGT ATGCGCGGGT GCTGCAGGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCGG ACGGACAGCC AGTGGTGCAC CACGGTTACA CGCTCACTTC AAAGATCCTC 
TTCAGAGATG CCGCGCACCC CATCAACAAG CATGCCTTTG TGAAGAACGA GTTTCCAGTC 
ATCCTGTCCA TTGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAAATTGC TCAGTACCTG 
AAAGGAATAT TCCAAGACAA ACTCGACCTG TCATCTATAG ACACAGGAGA GACCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAC 
CACCTTGGAG ATGATGCTGA AGAAGGAGAA GTTTCTGATG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCGCTG TTAAAAGAAT CTCAAATTCG AGACAAGGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTC CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGCATCCAGA TGCAAAGGAA AGTGGAAAGA AAGCACATGG ACGATCCCTC 
ATGACCAACT TCGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAATC TTATAGTACT 
GATGATGAGG AGGACGCACA GCAGAATCCT GGCAAGGAAA CTGGCCAGCT GTACCGGTTG 
GGCCGCCGAA GGAAAACCAT GAAGCTGTGT CGAGAGCTCT CTGATTTGGT TGTGTACACA 
AACTCTGTGG CAGCCCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCCTTC 
AGTGAAACAA GAGCACATCA AGTGGTTCAG CAGAAATCAG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTATCCCTCC GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGTTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAACG GCAATTGCGG CTACGTTCTC 
AAACCCCAGC AGATGTGCAA AGGTACCTTC AACCCTTTCT CAGGTGATCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT CCTGAAAGTG ATCAGTGGAC AACAACTCCC CAAACCTCCA 
GATTCCATGT TTGGAGACCG AGGAGAGATC ATCGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTC TAAAGATCAA ACTCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTATGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTCCGG 
TTCCTCGTGT GGGATCATGA TCCAATTGGA CGAGACTTTG TTGGGCAAAG AACTGTGACC 
TTCAGCAGCT TGGTGCCTGG CTACCGGCAT GTCTACTTGG AAGGACTGAC AGAAGCATCT 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTTTG 
AAGGGGCTGT TCAATAAGAA TCCCAGACAC AATTCTTCAG AAAACACTTG CCATTACATT 
CGGAAGCGAT CGATTGGAGA TAAGATTCTG CGACGCACAG CCAGCGCTCC GGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TGAAGGATTC TGTTTCTGAG 
GCTGCAAGAG ATCAAGATGG GGTCCTGAGA AGGACCACGC GGAGTTTGCA AGCGCGCCCT 
GTCTCTATGC CTGTTGACAG AAGTCTTCTT GGGGCTCTGT CGCTGCCTGT ATCTGAAACC 
ACAAAAGACA CTGAAGGAAA GGAAAACTCT CAAGCAGAAG ATAAGGATGC CAGGAGAGAT 
GGGAAGGCAA GTGTAAAAGA CCAGCATCTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCCAGCGCTC TCATCCACAA AGACATCAGC CAAGGGAACT CTGCTGTTTC TACTGCCAAC 
TTGTCAATCA CAGAACAGTT AGTGCCAGGT CCAAAGGGTG GGAGAACCAA ATCAAACATG 
TTAAGTGATT GCTGGGAACA TCAGTGCCTC AGCAGATCCC TCTCTCCAAG GCAGCATTTG 
GCTCATGATC CTGCAGTTAG CCCATCAAAA GATCTATGTG GTGTGAAAAC CAAGGAAAAT 
GGGAATGCTG GGGGCTTCGT GGTAGGGAAA AGCACATTGT CAGGAAGCAT CCTTTCTCAA 
AGCAATCTGG AGATTAAGAA GCTGGAAGGT AACTGGGATA AGGGCAGAGC TGCAACATCC 
TTTTCTTTGT CAGACGTCTC TACACTCTGT TCTGATGCAC CAGACTTACA TTCCACTGCA 
ATTCTCCAGG AGAGTGAAAT TTCCCACCTT ATTGACAATG TCACTTTAAC AAATGAGAAT 
GAACCTGGCA GTTCCATCTC AGCACTGATT GGCCAGTTTG ATGAGACCGG CAACCAGGCT 
AACCCTACAG TTGTTTCTCA TCTTCAGAGC CCCAGTGTGA TGTCGGGCCA TCCTCCTGTG 
CCCACCATGG ACCTAAAAAT GCCCTTCAAG CCTGGCTTTT CCAATGGAAA ACCCAAGTCA 
TCCTTCCTGT GCTCGTCTCC TGAGCAGATT GCACTCTCTG GACACGAGAC CTGTGAATGT 
TCAACACACA CAGCTGTTGG TGAAACTATG TGCACTCCTG TCTCTAAAAT GAAACCAGAC 
AATGACCTTT CTGGGAAGGC CATGACTGGG GCCATAGAAA ACAACCTGCC TCAGTCCTCT 
AATACTTCTC ACTGCTGGTT ACCAGAAAGT CCCACCCATG GGAAAGACTG GGAAATCCTG 
AAGAACCCCA GCCCTGCCAC TTCCACTGAC TTGACACTGG AGGATGTAAT AGCTGCTCCT 
ACTCTCAGTT TAAATTCTGG GGAGAGCAGT CTCGTAGAAA TGGATGGAGA CTCAGAAAAT 
CTGTCCCTAA CAACCTATGA ATATAGGAGA GAGGGCACAA GTCACCTCAC TTCTCCTTTA 
AAAGTGAAAT ACAGTCCAGC TGCAGTGGAA CATTTTCAAA GAGGCTTGAG AAATGGCTAC 
TGTAAAGAGA CTCTCCACCC TTCTGTCTCT GAAATATTCA ACAATACTCA AGATGTCAAA 
AATCAAAATA TCTCTCATCT AGCCTATCAG GGTGCTGGGT TTATGCATAA TCACTTCTCA 
AATTCAGATG CAAAAACGAA CCAGACCTCT GGGCCCCTGC CTTGTGCTCA TGACTTGCAT 
GCCCCTGCCC CGGAGCAGTC CACACACTCT GCTCTGAAGC TGTCCAGTCC GTGCAAATCC 
AAAAGTCTTG GGGACTTAAC ATCAGAGGAC ATCGCCTGTA ATTTTGAAAG CAAGTACCAG 
TGTATTAGTA AGAGCTTCGT TACCACTGGC ATCAGAGACA AGAAGGGCAT GACTGTAAAA 
ACAAAGTCGT TAGAGCCTAT GGATGCCCTG ACCGAGCAGC TGCGGAAACT TGTGTCCTTT 
GACCAGGAAG ATGGCTGCCA AGTGCTGTAC TCCAAGCAAG ATGCCAATCA ATTCCCCAGG 
GCCTTGGTCA GAAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AGCAGGAAGC CAACAGGCAG AAGAGTGTGA ACCCCAGCAC TGTGGGAGGA 
GTGGTTCTTC GAAGCAAGCC GTGCGCGCCG GCCCCCACAG GGAACAGGCA CTCCACCGGT 
TCCTACATCG CGGGCTACCT GCGGAGCGCC AAGGGCGGCA GCTTGGAGGG CCGCGGCATC 
CCCGAGGGCG CATGCGCAGC CCTGCGCTCC GGCCACGTCG ACAGGTTCTG TTCACATCAT 
TCTGTTCTGC AGACCGAACC CAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCH1
Entrez Gene ID	513565
Full Name	phospholipase C eta 1
General protein information	Preferred Names phospholipase C eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCH1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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