PPP1R21 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R21
Entrez Gene ID	513495
Full Name	protein phosphatase 1 regulatory subunit 21
Synonyms	CCDC128,KLRAQ1
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 21 Names protein phosphatase 1 regulatory subunit 21 KLRAQ motif containing 1 coiled-coil domain containing 128
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473432.1	XP_015328918.1	protein phosphatase 1 regulatory subunit 21 isoform X1
XM_015473432.2	XP_015328918.1	protein phosphatase 1 regulatory subunit 21 isoform X2
NM_001103093.1	NP_001096563.1	protein phosphatase 1 regulatory subunit 21
XM_024998536.1	XP_024854304.1	protein phosphatase 1 regulatory subunit 21 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ppp1r21	NP_082934.3
Gallus gallus (chicken)	PPP1R21	NP_001012891.1
Pan troglodytes (chimpanzee)	KLRAQ1	XP_001151290.1
Canis lupus familiaris (dog)	PPP1R21	XP_531815.2
Bos taurus (cattle)	PPP1R21	NP_001096563.1
Rattus norvegicus (Norway rat)	Ppp1r21	NP_001258234.1
Danio rerio (zebrafish)	ppp1r21	NP_001007300.1
	KLRAQ1	XP_001114046.1
Homo sapiens (human)	PPP1R21	NP_001129101.1
Xenopus tropicalis (tropical clawed frog)	ppp1r21	XP_002935419.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPP1R21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36812	NM_001103093.1 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa23914	XM_015473432.1 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa220283	XM_024998536.1 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220284	XM_015473432.2 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R21 ( NM_001103093.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096563.1 Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), mRNA.

Price & Availability↑

CloneID	OBa36812
Clone ID Related Accession (Same CDS sequence)	NM_001103093.1
Accession Version	NM_001103093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory subunit 21
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140557.1. On Sep 4, 2007 this sequence version replaced XR_028427.1. ##Evidence-Data-START## Transcript exon combination :: BC140557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCGG CAGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCTAAGCTT 
CGGGCTCAGA ATCAGGTCTT GAAGAAAGGT GTCGTGGATG AACAAGCGAA TTCTGCTGCT 
TTGAAGGAAC AGCTGAAAAT GAAGGATCAA TCACTGAGGA AACTGCAGCA GGAAATGGAC 
AGTTTGACAT TCCGAAATCT GCAGCTTGCC AAAAGGGTCG AGCTCCTTCA AGATGAACTG 
GCTCTAAGTG AACCCCGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GACCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCACGTGGA AGCAGAGCTG 
AGGAGTCGGC TGTCCACTCT GGAAATGGAG GCGGCTCAGC ACCAGGCCGT GGTCGATGGC 
CTGACGCGGA AGTACATGGA GACCATCGAG AAACTGCAGA ACGACAAGGC TAAACTAGAG 
GTAAAATCTC AGACTCTAGA AAAGGAGGCC AAGGAATGTC GACTTCGGAC AGAAGAATGT 
CAGTTACAGT TAAAGAATCT TCATGAAGAT TTGTCAAGTA GATTAGAAGA ATCTTTATCA 
ATCATCAGTG AGAAAGTACC TTTTAATGAT ACAAAATATA GCCAGTATAA TGCTCTGAAT 
GTTCCAGTCC ACAATAGGAG ACATCAGCTG AAGATGCGGG ATATCGCTGG GCAGGCCCTG 
GCTTTTGTTC AAGATCTTGT CAAGGCTCTT CTGAACTTTC ACACCTATAC AGAACAGAGG 
ATTCAGATTT TTCCTATTGA TTCTGCCATT GACACAATAT CTCCATTGAA TCAGAAGTTC 
TCACAGTATC TTCATGAAAA TGCATCTTAC GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATTACTGA GGATACTGTA ACCGTCCTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AGCATTTAAC CTCTTACATA TGTTTTCTTA GGAAGATTCT TCCTTATCAA 
TTAAAAAGCC TAGAAGAAGA ATGTGAAGCT TCCCTTTGCA CGTCCGCATT ACGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAG ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATTGCCC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTGTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TCCATGATGT CATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAGGCTACA ATAGAGCTTG AACTTCCAAC AACAACCCAG 
AAGCTCATAA CAACTAATGA CTGCATCCTG TCATCAGTAG TGGCATTGAC AAATGGAGCA 
GGAAAGATTG CATCTTTCTT CAGCAACAAC GTGGACTACT TCATTGCTTC CCTGAGCTAT 
GGGCCCAAGA CAGGGAGTGG ATTCATCAGT CCTCTTTCAG CCGAATGCAT GCTGCAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGGCT TTGAGAAAGC CCCTCTTGGA ATCTGTGCCT 
TATGAAGAAG CTCTGGCAAA CCGCCGGGTC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCGCAAC AGGTTCAGCA GAGTTTGGAG AAGATTTCTA AACTGGAGCA GGAAAAGGAA 
CATTGGATGT TGGAAGCACA GTTAGCTAAA ATCAAGCTGG AGAAAGAAAA CCAGCGAATT 
GCAGATAAAC TGAAGAGTAC AAGTAGTGGG CAGGTGGTAG GGGTGGCCCA GGAAAAGGCT 
GCTGTGGCGA CTGCTACGGG CCAGGAAGAA GCATCCGCTA AGGCTGTGCC AGAACCCGTC 
CACAGCACCA GTGAGATTGG AATTTTAACC AGGACATCTG ACAGTGAGGC CCCAGATGTG 
GAGTCTCGGG AAGACTTAAT CAGAAATCAC TACATGACAA GGATAGTGGA ACTGACGTCT 
CAGCTGCAGC TAGCTGACAG CAAGTCAGTG CACTTTTATG CTGAGTGCCG AGCACTCTCT 
AAAAGGCTGG CCTTGGCTGA AAAGTCTAAA GAGGCACTGA CAGAAGAGAT GAAAGTAGCC 
AGTCAGAGCA TTAGCCGACT TCAGGATGAG CTGACAACTA CCAAGAGGAG TTACGAGGAT 
CAGTTAAGTA TGATGAGCGA CCACCTGTGC AGCATGAACG AGACGCTATC TAAACAAAGA 
GAGGAGATTG ACACACTAAA GATGTCCAGT AAGGGAAATT CTAAAAAGAA CAAGAGTCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096563.1
CDS	125..2467
Translation	MASAELQGKYQKLAQEYSKLRAQNQVLKKGVVDEQANSAALKEQLKMKDQSLRKLQQEMDSLTFRNLQLAKRVELLQDELALSEPRGKKNKKSGESSSQLSQEQKSVFDEDLQKKIEENERLHIQFFEADEQHKHVEAELRSRLSTLEMEAAQHQAVVDGLTRKYMETIEKLQNDKAKLEVKSQTLEKEAKECRLRTEECQLQLKNLHEDLSSRLEESLSIISEKVPFNDTKYSQYNALNVPVHNRRHQLKMRDIAGQALAFVQDLVKALLNFHTYTEQRIQIFPIDSAIDTISPLNQKFSQYLHENASYVRPLEEGMLHLFESITEDTVTVLETTVKLKTFSEHLTSYICFLRKILPYQLKSLEEECEASLCTSALRARNLELSQDMKKMTAVFEKLQTYIALLALPSTEPDGLLRTNYSCVLTNVGAALHGFHDVMKDISKHYSQKATIELELPTTTQKLITTNDCILSSVVALTNGAGKIASFFSNNVDYFIASLSYGPKTGSGFISPLSAECMLQYKKKAAAYMKALRKPLLESVPYEEALANRRVLLSSTESREGLAQQVQQSLEKISKLEQEKEHWMLEAQLAKIKLEKENQRIADKLKSTSSGQVVGVAQEKAAVATATGQEEASAKAVPEPVHSTSEIGILTRTSDSEAPDVESREDLIRNHYMTRIVELTSQLQLADSKSVHFYAECRALSKRLALAEKSKEALTEEMKVASQSISRLQDELTTTKRSYEDQLSMMSDHLCSMNETLSKQREEIDTLKMSSKGNSKKNKSR

Target ORF information:

RefSeq Version	NM_001103093.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103093.1

ORF Insert Sequence:

ATGGCCTCGG CAGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCTAAGCTT 
CGGGCTCAGA ATCAGGTCTT GAAGAAAGGT GTCGTGGATG AACAAGCGAA TTCTGCTGCT 
TTGAAGGAAC AGCTGAAAAT GAAGGATCAA TCACTGAGGA AACTGCAGCA GGAAATGGAC 
AGTTTGACAT TCCGAAATCT GCAGCTTGCC AAAAGGGTCG AGCTCCTTCA AGATGAACTG 
GCTCTAAGTG AACCCCGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GACCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCACGTGGA AGCAGAGCTG 
AGGAGTCGGC TGTCCACTCT GGAAATGGAG GCGGCTCAGC ACCAGGCCGT GGTCGATGGC 
CTGACGCGGA AGTACATGGA GACCATCGAG AAACTGCAGA ACGACAAGGC TAAACTAGAG 
GTAAAATCTC AGACTCTAGA AAAGGAGGCC AAGGAATGTC GACTTCGGAC AGAAGAATGT 
CAGTTACAGT TAAAGAATCT TCATGAAGAT TTGTCAAGTA GATTAGAAGA ATCTTTATCA 
ATCATCAGTG AGAAAGTACC TTTTAATGAT ACAAAATATA GCCAGTATAA TGCTCTGAAT 
GTTCCAGTCC ACAATAGGAG ACATCAGCTG AAGATGCGGG ATATCGCTGG GCAGGCCCTG 
GCTTTTGTTC AAGATCTTGT CAAGGCTCTT CTGAACTTTC ACACCTATAC AGAACAGAGG 
ATTCAGATTT TTCCTATTGA TTCTGCCATT GACACAATAT CTCCATTGAA TCAGAAGTTC 
TCACAGTATC TTCATGAAAA TGCATCTTAC GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATTACTGA GGATACTGTA ACCGTCCTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AGCATTTAAC CTCTTACATA TGTTTTCTTA GGAAGATTCT TCCTTATCAA 
TTAAAAAGCC TAGAAGAAGA ATGTGAAGCT TCCCTTTGCA CGTCCGCATT ACGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAG ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATTGCCC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTGTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TCCATGATGT CATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAGGCTACA ATAGAGCTTG AACTTCCAAC AACAACCCAG 
AAGCTCATAA CAACTAATGA CTGCATCCTG TCATCAGTAG TGGCATTGAC AAATGGAGCA 
GGAAAGATTG CATCTTTCTT CAGCAACAAC GTGGACTACT TCATTGCTTC CCTGAGCTAT 
GGGCCCAAGA CAGGGAGTGG ATTCATCAGT CCTCTTTCAG CCGAATGCAT GCTGCAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGGCT TTGAGAAAGC CCCTCTTGGA ATCTGTGCCT 
TATGAAGAAG CTCTGGCAAA CCGCCGGGTC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCGCAAC AGGTTCAGCA GAGTTTGGAG AAGATTTCTA AACTGGAGCA GGAAAAGGAA 
CATTGGATGT TGGAAGCACA GTTAGCTAAA ATCAAGCTGG AGAAAGAAAA CCAGCGAATT 
GCAGATAAAC TGAAGAGTAC AAGTAGTGGG CAGGTGGTAG GGGTGGCCCA GGAAAAGGCT 
GCTGTGGCGA CTGCTACGGG CCAGGAAGAA GCATCCGCTA AGGCTGTGCC AGAACCCGTC 
CACAGCACCA GTGAGATTGG AATTTTAACC AGGACATCTG ACAGTGAGGC CCCAGATGTG 
GAGTCTCGGG AAGACTTAAT CAGAAATCAC TACATGACAA GGATAGTGGA ACTGACGTCT 
CAGCTGCAGC TAGCTGACAG CAAGTCAGTG CACTTTTATG CTGAGTGCCG AGCACTCTCT 
AAAAGGCTGG CCTTGGCTGA AAAGTCTAAA GAGGCACTGA CAGAAGAGAT GAAAGTAGCC 
AGTCAGAGCA TTAGCCGACT TCAGGATGAG CTGACAACTA CCAAGAGGAG TTACGAGGAT 
CAGTTAAGTA TGATGAGCGA CCACCTGTGC AGCATGAACG AGACGCTATC TAAACAAAGA 
GAGGAGATTG ACACACTAAA GATGTCCAGT AAGGGAAATT CTAAAAAGAA CAAGAGTCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R21 ( XM_015473432.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328918.1 Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23914
Clone ID Related Accession (Same CDS sequence)	XM_015473432.1
Accession Version	XM_015473432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory subunit 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG AAAGCTTCGG 
GCTCAGAATC AGGTCTTGAA GAAAGGTGTC GTGGATGAAC AAGCGAATTC TGCTGCTTTG 
AAGGAACAGC TGAAAATGAA GGATCAATCA CTGAGGAAAC TGCAGCAGGA AATGGACAGT 
TTGACATTCC GAAATCTGCA GCTTGCCAAA AGGGTCGAGC TCCTTCAAGA TGAACTGGCT 
CTAAGTGAAC CCCGAGGCAA GAAAAACAAG AAAAGTGGAG AATCTTCTTC TCAGTTGAGT 
CAAGAGCAGA AGAGTGTCTT TGATGAAGAC CTGCAAAAGA AGATAGAAGA GAATGAACGG 
TTGCATATAC AATTTTTTGA AGCTGATGAG CAGCACAAGC ACGTGGAAGC AGAGCTGAGG 
AGTCGGCTGT CCACTCTGGA AATGGAGGCG GCTCAGCACC AGGCCGTGGT CGATGGCCTG 
ACGCGGAAGT ACATGGAGAC CATCGAGAAA CTGCAGAACG ACAAGGCTAA ACTAGAGGTA 
AAATCTCAGA CTCTAGAAAA GGAGGCCAAG GAATGTCGAC TTCGGACAGA AGAATGTCAG 
TTACAGTTAA AGAATCTTCA TGAAGATTTG TCAAGTAGAT TAGAAGAATC TTTATCAATC 
ATCAGTGAGA AAGTACCTTT TAATGATACA AAATATAGCC AGTATAATGC TCTGAATGTT 
CCAGTCCACA ATAGGAGACA TCAGCTGAAG ATGCGGGATA TCGCTGGGCA GGCCCTGGCT 
TTTGTTCAAG ATCTTGTCAA GGCTCTTCTG AACTTTCACA CCTATACAGA ACAGAGGATT 
CAGATTTTTC CTATTGATTC TGCCATTGAC ACAATATCTC CATTGAATCA GAAGTTCTCA 
CAGTATCTTC ATGAAAATGC ATCTTACGTC CGCCCTCTTG AGGAAGGAAT GCTTCATTTA 
TTTGAAAGTA TTACTGAGGA TACTGTAACC GTCCTGGAGA CAACTGTGAA ATTGAAAACT 
TTTTCAGAGC ATTTAACCTC TTACATATGT TTTCTTAGGA AGATTCTTCC TTATCAATTA 
AAAAGCCTAG AAGAAGAATG TGAAGCTTCC CTTTGCACGT CCGCATTACG AGCCAGGAAT 
CTAGAGCTGT CCCAGGACAT GAAAAAGATG ACAGCTGTGT TTGAGAAGCT GCAGACTTAC 
ATTGCCCTTC TTGCCTTGCC AAGTACAGAG CCAGATGGAC TCCTTCGGAC AAACTACAGT 
TGTGTGTTAA CAAATGTTGG TGCTGCTCTG CATGGATTCC ATGATGTCAT GAAAGATATT 
TCCAAACATT ATAGTCAAAA GGCTACAATA GAGCTTGAAC TTCCAACAAC AACCCAGAAG 
CTCATAACAA CTAATGACTG CATCCTGTCA TCAGTAGTGG CATTGACAAA TGGAGCAGGA 
AAGATTGCAT CTTTCTTCAG CAACAACGTG GACTACTTCA TTGCTTCCCT GAGCTATGGG 
CCCAAGACAG GGAGTGGATT CATCAGTCCT CTTTCAGCCG AATGCATGCT GCAGTATAAG 
AAAAAAGCTG CTGCCTATAT GAAGGCTTTG AGAAAGCCCC TCTTGGAATC TGTGCCTTAT 
GAAGAAGCTC TGGCAAACCG CCGGGTCCTT CTCAGCTCTA CTGAAAGTCG AGAAGGCCTT 
GCGCAACAGG TTCAGCAGAG TTTGGAGAAG ATTTCTAAAC TGGAGCAGGA AAAGGAACAT 
TGGATGTTGG AAGCACAGTT AGCTAAAATC AAGCTGGAGA AAGAAAACCA GCGAATTGCA 
GATAAACTGA AGAGTACAAG TAGTGGGCAG GTGGTAGGGG TGGCCCAGGA AAAGGCTGCT 
GTGGCGACTG CTACGGGCCA GGAAGAAGCA TCCGCTAAGG CTGTGCCAGA ACCCGTCCAC 
AGCACCAGTG AGATTGGAAT TTTAACCAGG ACATCTGACA GTGAGGCCCC AGATGTGGAG 
TCTCGGGAAG ACTTAATCAG AAATCACTAC ATGACAAGGA TAGTGGAACT GACGTCTCAG 
CTGCAGCTAG CTGACAGCAA GTCAGTGCAC TTTTATGCTG AGTGCCGAGC ACTCTCTAAA 
AGGCTGGCCT TGGCTGAAAA GTCTAAAGAG GCACTGACAG AAGAGATGAA AGTAGCCAGT 
CAGAGCATTA GCCGACTTCA GGATGAGCTG ACAACTACCA AGAGGAGTTA CGAGGATCAG 
TTAAGTATGA TGAGCGACCA CCTGTGCAGC ATGAACGAGA CGCTATCTAA ACAAAGAGAG 
GAGATTGACA CACTAAAGAT GTCCAGTAAG GGAAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328918.1
CDS	166..2505
Translation	MSEILASASGAGREGQGKLRAQNQVLKKGVVDEQANSAALKEQLKMKDQSLRKLQQEMDSLTFRNLQLAKRVELLQDELALSEPRGKKNKKSGESSSQLSQEQKSVFDEDLQKKIEENERLHIQFFEADEQHKHVEAELRSRLSTLEMEAAQHQAVVDGLTRKYMETIEKLQNDKAKLEVKSQTLEKEAKECRLRTEECQLQLKNLHEDLSSRLEESLSIISEKVPFNDTKYSQYNALNVPVHNRRHQLKMRDIAGQALAFVQDLVKALLNFHTYTEQRIQIFPIDSAIDTISPLNQKFSQYLHENASYVRPLEEGMLHLFESITEDTVTVLETTVKLKTFSEHLTSYICFLRKILPYQLKSLEEECEASLCTSALRARNLELSQDMKKMTAVFEKLQTYIALLALPSTEPDGLLRTNYSCVLTNVGAALHGFHDVMKDISKHYSQKATIELELPTTTQKLITTNDCILSSVVALTNGAGKIASFFSNNVDYFIASLSYGPKTGSGFISPLSAECMLQYKKKAAAYMKALRKPLLESVPYEEALANRRVLLSSTESREGLAQQVQQSLEKISKLEQEKEHWMLEAQLAKIKLEKENQRIADKLKSTSSGQVVGVAQEKAAVATATGQEEASAKAVPEPVHSTSEIGILTRTSDSEAPDVESREDLIRNHYMTRIVELTSQLQLADSKSVHFYAECRALSKRLALAEKSKEALTEEMKVASQSISRLQDELTTTKRSYEDQLSMMSDHLCSMNETLSKQREEIDTLKMSSKGNSKKNKSR

Target ORF information:

RefSeq Version	XM_015473432.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473432.1

ORF Insert Sequence:

ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG AAAGCTTCGG 
GCTCAGAATC AGGTCTTGAA GAAAGGTGTC GTGGATGAAC AAGCGAATTC TGCTGCTTTG 
AAGGAACAGC TGAAAATGAA GGATCAATCA CTGAGGAAAC TGCAGCAGGA AATGGACAGT 
TTGACATTCC GAAATCTGCA GCTTGCCAAA AGGGTCGAGC TCCTTCAAGA TGAACTGGCT 
CTAAGTGAAC CCCGAGGCAA GAAAAACAAG AAAAGTGGAG AATCTTCTTC TCAGTTGAGT 
CAAGAGCAGA AGAGTGTCTT TGATGAAGAC CTGCAAAAGA AGATAGAAGA GAATGAACGG 
TTGCATATAC AATTTTTTGA AGCTGATGAG CAGCACAAGC ACGTGGAAGC AGAGCTGAGG 
AGTCGGCTGT CCACTCTGGA AATGGAGGCG GCTCAGCACC AGGCCGTGGT CGATGGCCTG 
ACGCGGAAGT ACATGGAGAC CATCGAGAAA CTGCAGAACG ACAAGGCTAA ACTAGAGGTA 
AAATCTCAGA CTCTAGAAAA GGAGGCCAAG GAATGTCGAC TTCGGACAGA AGAATGTCAG 
TTACAGTTAA AGAATCTTCA TGAAGATTTG TCAAGTAGAT TAGAAGAATC TTTATCAATC 
ATCAGTGAGA AAGTACCTTT TAATGATACA AAATATAGCC AGTATAATGC TCTGAATGTT 
CCAGTCCACA ATAGGAGACA TCAGCTGAAG ATGCGGGATA TCGCTGGGCA GGCCCTGGCT 
TTTGTTCAAG ATCTTGTCAA GGCTCTTCTG AACTTTCACA CCTATACAGA ACAGAGGATT 
CAGATTTTTC CTATTGATTC TGCCATTGAC ACAATATCTC CATTGAATCA GAAGTTCTCA 
CAGTATCTTC ATGAAAATGC ATCTTACGTC CGCCCTCTTG AGGAAGGAAT GCTTCATTTA 
TTTGAAAGTA TTACTGAGGA TACTGTAACC GTCCTGGAGA CAACTGTGAA ATTGAAAACT 
TTTTCAGAGC ATTTAACCTC TTACATATGT TTTCTTAGGA AGATTCTTCC TTATCAATTA 
AAAAGCCTAG AAGAAGAATG TGAAGCTTCC CTTTGCACGT CCGCATTACG AGCCAGGAAT 
CTAGAGCTGT CCCAGGACAT GAAAAAGATG ACAGCTGTGT TTGAGAAGCT GCAGACTTAC 
ATTGCCCTTC TTGCCTTGCC AAGTACAGAG CCAGATGGAC TCCTTCGGAC AAACTACAGT 
TGTGTGTTAA CAAATGTTGG TGCTGCTCTG CATGGATTCC ATGATGTCAT GAAAGATATT 
TCCAAACATT ATAGTCAAAA GGCTACAATA GAGCTTGAAC TTCCAACAAC AACCCAGAAG 
CTCATAACAA CTAATGACTG CATCCTGTCA TCAGTAGTGG CATTGACAAA TGGAGCAGGA 
AAGATTGCAT CTTTCTTCAG CAACAACGTG GACTACTTCA TTGCTTCCCT GAGCTATGGG 
CCCAAGACAG GGAGTGGATT CATCAGTCCT CTTTCAGCCG AATGCATGCT GCAGTATAAG 
AAAAAAGCTG CTGCCTATAT GAAGGCTTTG AGAAAGCCCC TCTTGGAATC TGTGCCTTAT 
GAAGAAGCTC TGGCAAACCG CCGGGTCCTT CTCAGCTCTA CTGAAAGTCG AGAAGGCCTT 
GCGCAACAGG TTCAGCAGAG TTTGGAGAAG ATTTCTAAAC TGGAGCAGGA AAAGGAACAT 
TGGATGTTGG AAGCACAGTT AGCTAAAATC AAGCTGGAGA AAGAAAACCA GCGAATTGCA 
GATAAACTGA AGAGTACAAG TAGTGGGCAG GTGGTAGGGG TGGCCCAGGA AAAGGCTGCT 
GTGGCGACTG CTACGGGCCA GGAAGAAGCA TCCGCTAAGG CTGTGCCAGA ACCCGTCCAC 
AGCACCAGTG AGATTGGAAT TTTAACCAGG ACATCTGACA GTGAGGCCCC AGATGTGGAG 
TCTCGGGAAG ACTTAATCAG AAATCACTAC ATGACAAGGA TAGTGGAACT GACGTCTCAG 
CTGCAGCTAG CTGACAGCAA GTCAGTGCAC TTTTATGCTG AGTGCCGAGC ACTCTCTAAA 
AGGCTGGCCT TGGCTGAAAA GTCTAAAGAG GCACTGACAG AAGAGATGAA AGTAGCCAGT 
CAGAGCATTA GCCGACTTCA GGATGAGCTG ACAACTACCA AGAGGAGTTA CGAGGATCAG 
TTAAGTATGA TGAGCGACCA CCTGTGCAGC ATGAACGAGA CGCTATCTAA ACAAAGAGAG 
GAGATTGACA CACTAAAGAT GTCCAGTAAG GGAAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R21 ( XM_024998536.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854304.1 Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.

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CloneID	OBa220283
Clone ID Related Accession (Same CDS sequence)	XM_024998536.1
Accession Version	XM_024998536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory subunit 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGG ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG 
AAAGGTGAGG ACCATCGCTT TCCTGCTGCT TCCTCAATTT TCCCAGCCCG GCGATGGGAA 
GGATCAAGCT TTCTCCTTTC CCACAGGAAT GATCTTCGGG CTCAGAATCA GGTCTTGAAG 
AAAGGTGTCG TGGATGAACA AGCGAATTCT GCTGCTTTGA AGGAACAGCT GAAAATGAAG 
GATCAATCAC TGAGGAAACT GCAGCAGGAA ATGGACAGTT TGACATTCCG AAATCTGCAG 
CTTGCCAAAA GGGTCGAGCT CCTTCAAGAT GAACTGGCTC TAAGTGAACC CCGAGGCAAG 
AAAAACAAGA AAAGTGGAGA ATCTTCTTCT CAGTTGAGTC AAGAGCAGAA GAGTGTCTTT 
GATGAAGACC TGCAAAAGAA GATAGAAGAG AATGAACGGT TGCATATACA ATTTTTTGAA 
GCTGATGAGC AGCACAAGCA CGTGGAAGCA GAGCTGAGGA GTCGGCTGTC CACTCTGGAA 
ATGGAGGCGG CTCAGCACCA GGCCGTGGTC GATGGCCTGA CGCGGAAGTA CATGGAGACC 
ATCGAGAAAC TGCAGAACGA CAAGGCTAAA CTAGAGGTAA AATCTCAGAC TCTAGAAAAG 
GAGGCCAAGG AATGTCGACT TCGGACAGAA GAATGTCAGT TACAGTTAAA GAATCTTCAT 
GAAGATTTGT CAAGTAGATT AGAAGAATCT TTATCAATCA TCAGTGAGAA AGTACCTTTT 
AATGATACAA AATATAGCCA GTATAATGCT CTGAATGTTC CAGTCCACAA TAGGAGACAT 
CAGCTGAAGA TGCGGGATAT CGCTGGGCAG GCCCTGGCTT TTGTTCAAGA TCTTGTCAAG 
GCTCTTCTGA ACTTTCACAC CTATACAGAA CAGAGGATTC AGATTTTTCC TATTGATTCT 
GCCATTGACA CAATATCTCC ATTGAATCAG AAGTTCTCAC AGTATCTTCA TGAAAATGCA 
TCTTACGTCC GCCCTCTTGA GGAAGGAATG CTTCATTTAT TTGAAAGTAT TACTGAGGAT 
ACTGTAACCG TCCTGGAGAC AACTGTGAAA TTGAAAACTT TTTCAGAGCA TTTAACCTCT 
TACATATGTT TTCTTAGGAA GATTCTTCCT TATCAATTAA AAAGCCTAGA AGAAGAATGT 
GAAGCTTCCC TTTGCACGTC CGCATTACGA GCCAGGAATC TAGAGCTGTC CCAGGACATG 
AAAAAGATGA CAGCTGTGTT TGAGAAGCTG CAGACTTACA TTGCCCTTCT TGCCTTGCCA 
AGTACAGAGC CAGATGGACT CCTTCGGACA AACTACAGTT GTGTGTTAAC AAATGTTGGT 
GCTGCTCTGC ATGGATTCCA TGATGTCATG AAAGATATTT CCAAACATTA TAGTCAAAAG 
GCTACAATAG AGCTTGAACT TCCAACAACA ACCCAGAAGC TCATAACAAC TAATGACTGC 
ATCCTGTCAT CAGTAGTGGC ATTGACAAAT GGAGCAGGAA AGATTGCATC TTTCTTCAGC 
AACAACGTGG ACTACTTCAT TGCTTCCCTG AGCTATGGGC CCAAGACAGG GAGTGGATTC 
ATCAGTCCTC TTTCAGCCGA ATGCATGCTG CAGTATAAGA AAAAAGCTGC TGCCTATATG 
AAGGCTTTGA GAAAGCCCCT CTTGGAATCT GTGCCTTATG AAGAAGCTCT GGCAAACCGC 
CGGGTCCTTC TCAGCTCTAC TGAAAGTCGA GAAGGCCTTG CGCAACAGGT TCAGCAGAGT 
TTGGAGAAGA TTTCTAAACT GGAGCAGGAA AAGGAACATT GGATGTTGGA AGCACAGTTA 
GCTAAAATCA AGCTGGAGAA AGAAAACCAG CGAATTGCAG ATAAACTGAA GAGTACAAGT 
AGTGGGCAGG TGGTAGGGGT GGCCCAGGAA AAGGCTGCTG TGGCGACTGC TACGGGCCAG 
GAAGAAGCAT CCGCTAAGGC TGTGCCAGAA CCCGTCCACA GCACCAGTGA GATTGGAATT 
TTAACCAGGA CATCTGACAG TGAGGCCCCA GATGTGGAGT CTCGGGAAGA CTTAATCAGA 
AATCACTACA TGACAAGGAT AGTGGAACTG ACGTCTCAGC TGCAGCTAGC TGACAGCAAG 
TCAGTGCACT TTTATGCTGA GTGCCGAGCA CTCTCTAAAA GGCTGGCCTT GGCTGAAAAG 
TCTAAAGAGG CACTGACAGA AGAGATGAAA GTAGCCAGTC AGAGCATTAG CCGACTTCAG 
GATGAGCTGA CAACTACCAA GAGGAGTTAT GAGGATCAGT TAAGTATGAT GAGCGACCAC 
CTGTGCAGCA TGAACGAGAC GCTATCTAAA CAAAGAGAGG AGATTGACAC ACTAAAGATG 
TCCAGTAAGG GAAATTCTAA AAAGAACAAG AGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854304.1
CDS	175..2613
Translation	MDRDVRNPGFRLWCWSGGAGKGEDHRFPAASSIFPARRWEGSSFLLSHRNDLRAQNQVLKKGVVDEQANSAALKEQLKMKDQSLRKLQQEMDSLTFRNLQLAKRVELLQDELALSEPRGKKNKKSGESSSQLSQEQKSVFDEDLQKKIEENERLHIQFFEADEQHKHVEAELRSRLSTLEMEAAQHQAVVDGLTRKYMETIEKLQNDKAKLEVKSQTLEKEAKECRLRTEECQLQLKNLHEDLSSRLEESLSIISEKVPFNDTKYSQYNALNVPVHNRRHQLKMRDIAGQALAFVQDLVKALLNFHTYTEQRIQIFPIDSAIDTISPLNQKFSQYLHENASYVRPLEEGMLHLFESITEDTVTVLETTVKLKTFSEHLTSYICFLRKILPYQLKSLEEECEASLCTSALRARNLELSQDMKKMTAVFEKLQTYIALLALPSTEPDGLLRTNYSCVLTNVGAALHGFHDVMKDISKHYSQKATIELELPTTTQKLITTNDCILSSVVALTNGAGKIASFFSNNVDYFIASLSYGPKTGSGFISPLSAECMLQYKKKAAAYMKALRKPLLESVPYEEALANRRVLLSSTESREGLAQQVQQSLEKISKLEQEKEHWMLEAQLAKIKLEKENQRIADKLKSTSSGQVVGVAQEKAAVATATGQEEASAKAVPEPVHSTSEIGILTRTSDSEAPDVESREDLIRNHYMTRIVELTSQLQLADSKSVHFYAECRALSKRLALAEKSKEALTEEMKVASQSISRLQDELTTTKRSYEDQLSMMSDHLCSMNETLSKQREEIDTLKMSSKGNSKKNKSR

Target ORF information:

RefSeq Version	XM_024998536.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998536.1

ORF Insert Sequence:

ATGGACAGGG ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG 
AAAGGTGAGG ACCATCGCTT TCCTGCTGCT TCCTCAATTT TCCCAGCCCG GCGATGGGAA 
GGATCAAGCT TTCTCCTTTC CCACAGGAAT GATCTTCGGG CTCAGAATCA GGTCTTGAAG 
AAAGGTGTCG TGGATGAACA AGCGAATTCT GCTGCTTTGA AGGAACAGCT GAAAATGAAG 
GATCAATCAC TGAGGAAACT GCAGCAGGAA ATGGACAGTT TGACATTCCG AAATCTGCAG 
CTTGCCAAAA GGGTCGAGCT CCTTCAAGAT GAACTGGCTC TAAGTGAACC CCGAGGCAAG 
AAAAACAAGA AAAGTGGAGA ATCTTCTTCT CAGTTGAGTC AAGAGCAGAA GAGTGTCTTT 
GATGAAGACC TGCAAAAGAA GATAGAAGAG AATGAACGGT TGCATATACA ATTTTTTGAA 
GCTGATGAGC AGCACAAGCA CGTGGAAGCA GAGCTGAGGA GTCGGCTGTC CACTCTGGAA 
ATGGAGGCGG CTCAGCACCA GGCCGTGGTC GATGGCCTGA CGCGGAAGTA CATGGAGACC 
ATCGAGAAAC TGCAGAACGA CAAGGCTAAA CTAGAGGTAA AATCTCAGAC TCTAGAAAAG 
GAGGCCAAGG AATGTCGACT TCGGACAGAA GAATGTCAGT TACAGTTAAA GAATCTTCAT 
GAAGATTTGT CAAGTAGATT AGAAGAATCT TTATCAATCA TCAGTGAGAA AGTACCTTTT 
AATGATACAA AATATAGCCA GTATAATGCT CTGAATGTTC CAGTCCACAA TAGGAGACAT 
CAGCTGAAGA TGCGGGATAT CGCTGGGCAG GCCCTGGCTT TTGTTCAAGA TCTTGTCAAG 
GCTCTTCTGA ACTTTCACAC CTATACAGAA CAGAGGATTC AGATTTTTCC TATTGATTCT 
GCCATTGACA CAATATCTCC ATTGAATCAG AAGTTCTCAC AGTATCTTCA TGAAAATGCA 
TCTTACGTCC GCCCTCTTGA GGAAGGAATG CTTCATTTAT TTGAAAGTAT TACTGAGGAT 
ACTGTAACCG TCCTGGAGAC AACTGTGAAA TTGAAAACTT TTTCAGAGCA TTTAACCTCT 
TACATATGTT TTCTTAGGAA GATTCTTCCT TATCAATTAA AAAGCCTAGA AGAAGAATGT 
GAAGCTTCCC TTTGCACGTC CGCATTACGA GCCAGGAATC TAGAGCTGTC CCAGGACATG 
AAAAAGATGA CAGCTGTGTT TGAGAAGCTG CAGACTTACA TTGCCCTTCT TGCCTTGCCA 
AGTACAGAGC CAGATGGACT CCTTCGGACA AACTACAGTT GTGTGTTAAC AAATGTTGGT 
GCTGCTCTGC ATGGATTCCA TGATGTCATG AAAGATATTT CCAAACATTA TAGTCAAAAG 
GCTACAATAG AGCTTGAACT TCCAACAACA ACCCAGAAGC TCATAACAAC TAATGACTGC 
ATCCTGTCAT CAGTAGTGGC ATTGACAAAT GGAGCAGGAA AGATTGCATC TTTCTTCAGC 
AACAACGTGG ACTACTTCAT TGCTTCCCTG AGCTATGGGC CCAAGACAGG GAGTGGATTC 
ATCAGTCCTC TTTCAGCCGA ATGCATGCTG CAGTATAAGA AAAAAGCTGC TGCCTATATG 
AAGGCTTTGA GAAAGCCCCT CTTGGAATCT GTGCCTTATG AAGAAGCTCT GGCAAACCGC 
CGGGTCCTTC TCAGCTCTAC TGAAAGTCGA GAAGGCCTTG CGCAACAGGT TCAGCAGAGT 
TTGGAGAAGA TTTCTAAACT GGAGCAGGAA AAGGAACATT GGATGTTGGA AGCACAGTTA 
GCTAAAATCA AGCTGGAGAA AGAAAACCAG CGAATTGCAG ATAAACTGAA GAGTACAAGT 
AGTGGGCAGG TGGTAGGGGT GGCCCAGGAA AAGGCTGCTG TGGCGACTGC TACGGGCCAG 
GAAGAAGCAT CCGCTAAGGC TGTGCCAGAA CCCGTCCACA GCACCAGTGA GATTGGAATT 
TTAACCAGGA CATCTGACAG TGAGGCCCCA GATGTGGAGT CTCGGGAAGA CTTAATCAGA 
AATCACTACA TGACAAGGAT AGTGGAACTG ACGTCTCAGC TGCAGCTAGC TGACAGCAAG 
TCAGTGCACT TTTATGCTGA GTGCCGAGCA CTCTCTAAAA GGCTGGCCTT GGCTGAAAAG 
TCTAAAGAGG CACTGACAGA AGAGATGAAA GTAGCCAGTC AGAGCATTAG CCGACTTCAG 
GATGAGCTGA CAACTACCAA GAGGAGTTAT GAGGATCAGT TAAGTATGAT GAGCGACCAC 
CTGTGCAGCA TGAACGAGAC GCTATCTAAA CAAAGAGAGG AGATTGACAC ACTAAAGATG 
TCCAGTAAGG GAAATTCTAA AAAGAACAAG AGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R21 ( XM_015473432.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328918.1 Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X2, mRNA.

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CloneID	OBa220284
Clone ID Related Accession (Same CDS sequence)	XM_015473432.2
Accession Version	XM_015473432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory subunit 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG AAAGCTTCGG 
GCTCAGAATC AGGTCTTGAA GAAAGGTGTC GTGGATGAAC AAGCGAATTC TGCTGCTTTG 
AAGGAACAGC TGAAAATGAA GGATCAATCA CTGAGGAAAC TGCAGCAGGA AATGGACAGT 
TTGACATTCC GAAATCTGCA GCTTGCCAAA AGGGTCGAGC TCCTTCAAGA TGAACTGGCT 
CTAAGTGAAC CCCGAGGCAA GAAAAACAAG AAAAGTGGAG AATCTTCTTC TCAGTTGAGT 
CAAGAGCAGA AGAGTGTCTT TGATGAAGAC CTGCAAAAGA AGATAGAAGA GAATGAACGG 
TTGCATATAC AATTTTTTGA AGCTGATGAG CAGCACAAGC ACGTGGAAGC AGAGCTGAGG 
AGTCGGCTGT CCACTCTGGA AATGGAGGCG GCTCAGCACC AGGCCGTGGT CGATGGCCTG 
ACGCGGAAGT ACATGGAGAC CATCGAGAAA CTGCAGAACG ACAAGGCTAA ACTAGAGGTA 
AAATCTCAGA CTCTAGAAAA GGAGGCCAAG GAATGTCGAC TTCGGACAGA AGAATGTCAG 
TTACAGTTAA AGAATCTTCA TGAAGATTTG TCAAGTAGAT TAGAAGAATC TTTATCAATC 
ATCAGTGAGA AAGTACCTTT TAATGATACA AAATATAGCC AGTATAATGC TCTGAATGTT 
CCAGTCCACA ATAGGAGACA TCAGCTGAAG ATGCGGGATA TCGCTGGGCA GGCCCTGGCT 
TTTGTTCAAG ATCTTGTCAA GGCTCTTCTG AACTTTCACA CCTATACAGA ACAGAGGATT 
CAGATTTTTC CTATTGATTC TGCCATTGAC ACAATATCTC CATTGAATCA GAAGTTCTCA 
CAGTATCTTC ATGAAAATGC ATCTTACGTC CGCCCTCTTG AGGAAGGAAT GCTTCATTTA 
TTTGAAAGTA TTACTGAGGA TACTGTAACC GTCCTGGAGA CAACTGTGAA ATTGAAAACT 
TTTTCAGAGC ATTTAACCTC TTACATATGT TTTCTTAGGA AGATTCTTCC TTATCAATTA 
AAAAGCCTAG AAGAAGAATG TGAAGCTTCC CTTTGCACGT CCGCATTACG AGCCAGGAAT 
CTAGAGCTGT CCCAGGACAT GAAAAAGATG ACAGCTGTGT TTGAGAAGCT GCAGACTTAC 
ATTGCCCTTC TTGCCTTGCC AAGTACAGAG CCAGATGGAC TCCTTCGGAC AAACTACAGT 
TGTGTGTTAA CAAATGTTGG TGCTGCTCTG CATGGATTCC ATGATGTCAT GAAAGATATT 
TCCAAACATT ATAGTCAAAA GGCTACAATA GAGCTTGAAC TTCCAACAAC AACCCAGAAG 
CTCATAACAA CTAATGACTG CATCCTGTCA TCAGTAGTGG CATTGACAAA TGGAGCAGGA 
AAGATTGCAT CTTTCTTCAG CAACAACGTG GACTACTTCA TTGCTTCCCT GAGCTATGGG 
CCCAAGACAG GGAGTGGATT CATCAGTCCT CTTTCAGCCG AATGCATGCT GCAGTATAAG 
AAAAAAGCTG CTGCCTATAT GAAGGCTTTG AGAAAGCCCC TCTTGGAATC TGTGCCTTAT 
GAAGAAGCTC TGGCAAACCG CCGGGTCCTT CTCAGCTCTA CTGAAAGTCG AGAAGGCCTT 
GCGCAACAGG TTCAGCAGAG TTTGGAGAAG ATTTCTAAAC TGGAGCAGGA AAAGGAACAT 
TGGATGTTGG AAGCACAGTT AGCTAAAATC AAGCTGGAGA AAGAAAACCA GCGAATTGCA 
GATAAACTGA AGAGTACAAG TAGTGGGCAG GTGGTAGGGG TGGCCCAGGA AAAGGCTGCT 
GTGGCGACTG CTACGGGCCA GGAAGAAGCA TCCGCTAAGG CTGTGCCAGA ACCCGTCCAC 
AGCACCAGTG AGATTGGAAT TTTAACCAGG ACATCTGACA GTGAGGCCCC AGATGTGGAG 
TCTCGGGAAG ACTTAATCAG AAATCACTAC ATGACAAGGA TAGTGGAACT GACGTCTCAG 
CTGCAGCTAG CTGACAGCAA GTCAGTGCAC TTTTATGCTG AGTGCCGAGC ACTCTCTAAA 
AGGCTGGCCT TGGCTGAAAA GTCTAAAGAG GCACTGACAG AAGAGATGAA AGTAGCCAGT 
CAGAGCATTA GCCGACTTCA GGATGAGCTG ACAACTACCA AGAGGAGTTA TGAGGATCAG 
TTAAGTATGA TGAGCGACCA CCTGTGCAGC ATGAACGAGA CGCTATCTAA ACAAAGAGAG 
GAGATTGACA CACTAAAGAT GTCCAGTAAG GGAAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328918.1
CDS	184..2523
Translation	MSEILASASGAGREGQGKLRAQNQVLKKGVVDEQANSAALKEQLKMKDQSLRKLQQEMDSLTFRNLQLAKRVELLQDELALSEPRGKKNKKSGESSSQLSQEQKSVFDEDLQKKIEENERLHIQFFEADEQHKHVEAELRSRLSTLEMEAAQHQAVVDGLTRKYMETIEKLQNDKAKLEVKSQTLEKEAKECRLRTEECQLQLKNLHEDLSSRLEESLSIISEKVPFNDTKYSQYNALNVPVHNRRHQLKMRDIAGQALAFVQDLVKALLNFHTYTEQRIQIFPIDSAIDTISPLNQKFSQYLHENASYVRPLEEGMLHLFESITEDTVTVLETTVKLKTFSEHLTSYICFLRKILPYQLKSLEEECEASLCTSALRARNLELSQDMKKMTAVFEKLQTYIALLALPSTEPDGLLRTNYSCVLTNVGAALHGFHDVMKDISKHYSQKATIELELPTTTQKLITTNDCILSSVVALTNGAGKIASFFSNNVDYFIASLSYGPKTGSGFISPLSAECMLQYKKKAAAYMKALRKPLLESVPYEEALANRRVLLSSTESREGLAQQVQQSLEKISKLEQEKEHWMLEAQLAKIKLEKENQRIADKLKSTSSGQVVGVAQEKAAVATATGQEEASAKAVPEPVHSTSEIGILTRTSDSEAPDVESREDLIRNHYMTRIVELTSQLQLADSKSVHFYAECRALSKRLALAEKSKEALTEEMKVASQSISRLQDELTTTKRSYEDQLSMMSDHLCSMNETLSKQREEIDTLKMSSKGNSKKNKSR

Target ORF information:

RefSeq Version	XM_015473432.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473432.2

ORF Insert Sequence:

ATGTCAGAAA TCCTGGCTTC CGCCTCTGGT GCTGGTCGGG AGGGGCAGGG AAAGCTTCGG 
GCTCAGAATC AGGTCTTGAA GAAAGGTGTC GTGGATGAAC AAGCGAATTC TGCTGCTTTG 
AAGGAACAGC TGAAAATGAA GGATCAATCA CTGAGGAAAC TGCAGCAGGA AATGGACAGT 
TTGACATTCC GAAATCTGCA GCTTGCCAAA AGGGTCGAGC TCCTTCAAGA TGAACTGGCT 
CTAAGTGAAC CCCGAGGCAA GAAAAACAAG AAAAGTGGAG AATCTTCTTC TCAGTTGAGT 
CAAGAGCAGA AGAGTGTCTT TGATGAAGAC CTGCAAAAGA AGATAGAAGA GAATGAACGG 
TTGCATATAC AATTTTTTGA AGCTGATGAG CAGCACAAGC ACGTGGAAGC AGAGCTGAGG 
AGTCGGCTGT CCACTCTGGA AATGGAGGCG GCTCAGCACC AGGCCGTGGT CGATGGCCTG 
ACGCGGAAGT ACATGGAGAC CATCGAGAAA CTGCAGAACG ACAAGGCTAA ACTAGAGGTA 
AAATCTCAGA CTCTAGAAAA GGAGGCCAAG GAATGTCGAC TTCGGACAGA AGAATGTCAG 
TTACAGTTAA AGAATCTTCA TGAAGATTTG TCAAGTAGAT TAGAAGAATC TTTATCAATC 
ATCAGTGAGA AAGTACCTTT TAATGATACA AAATATAGCC AGTATAATGC TCTGAATGTT 
CCAGTCCACA ATAGGAGACA TCAGCTGAAG ATGCGGGATA TCGCTGGGCA GGCCCTGGCT 
TTTGTTCAAG ATCTTGTCAA GGCTCTTCTG AACTTTCACA CCTATACAGA ACAGAGGATT 
CAGATTTTTC CTATTGATTC TGCCATTGAC ACAATATCTC CATTGAATCA GAAGTTCTCA 
CAGTATCTTC ATGAAAATGC ATCTTACGTC CGCCCTCTTG AGGAAGGAAT GCTTCATTTA 
TTTGAAAGTA TTACTGAGGA TACTGTAACC GTCCTGGAGA CAACTGTGAA ATTGAAAACT 
TTTTCAGAGC ATTTAACCTC TTACATATGT TTTCTTAGGA AGATTCTTCC TTATCAATTA 
AAAAGCCTAG AAGAAGAATG TGAAGCTTCC CTTTGCACGT CCGCATTACG AGCCAGGAAT 
CTAGAGCTGT CCCAGGACAT GAAAAAGATG ACAGCTGTGT TTGAGAAGCT GCAGACTTAC 
ATTGCCCTTC TTGCCTTGCC AAGTACAGAG CCAGATGGAC TCCTTCGGAC AAACTACAGT 
TGTGTGTTAA CAAATGTTGG TGCTGCTCTG CATGGATTCC ATGATGTCAT GAAAGATATT 
TCCAAACATT ATAGTCAAAA GGCTACAATA GAGCTTGAAC TTCCAACAAC AACCCAGAAG 
CTCATAACAA CTAATGACTG CATCCTGTCA TCAGTAGTGG CATTGACAAA TGGAGCAGGA 
AAGATTGCAT CTTTCTTCAG CAACAACGTG GACTACTTCA TTGCTTCCCT GAGCTATGGG 
CCCAAGACAG GGAGTGGATT CATCAGTCCT CTTTCAGCCG AATGCATGCT GCAGTATAAG 
AAAAAAGCTG CTGCCTATAT GAAGGCTTTG AGAAAGCCCC TCTTGGAATC TGTGCCTTAT 
GAAGAAGCTC TGGCAAACCG CCGGGTCCTT CTCAGCTCTA CTGAAAGTCG AGAAGGCCTT 
GCGCAACAGG TTCAGCAGAG TTTGGAGAAG ATTTCTAAAC TGGAGCAGGA AAAGGAACAT 
TGGATGTTGG AAGCACAGTT AGCTAAAATC AAGCTGGAGA AAGAAAACCA GCGAATTGCA 
GATAAACTGA AGAGTACAAG TAGTGGGCAG GTGGTAGGGG TGGCCCAGGA AAAGGCTGCT 
GTGGCGACTG CTACGGGCCA GGAAGAAGCA TCCGCTAAGG CTGTGCCAGA ACCCGTCCAC 
AGCACCAGTG AGATTGGAAT TTTAACCAGG ACATCTGACA GTGAGGCCCC AGATGTGGAG 
TCTCGGGAAG ACTTAATCAG AAATCACTAC ATGACAAGGA TAGTGGAACT GACGTCTCAG 
CTGCAGCTAG CTGACAGCAA GTCAGTGCAC TTTTATGCTG AGTGCCGAGC ACTCTCTAAA 
AGGCTGGCCT TGGCTGAAAA GTCTAAAGAG GCACTGACAG AAGAGATGAA AGTAGCCAGT 
CAGAGCATTA GCCGACTTCA GGATGAGCTG ACAACTACCA AGAGGAGTTA TGAGGATCAG 
TTAAGTATGA TGAGCGACCA CCTGTGCAGC ATGAACGAGA CGCTATCTAA ACAAAGAGAG 
GAGATTGACA CACTAAAGAT GTCCAGTAAG GGAAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PPP1R21 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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