EDC4 cDNA ORF clone, Bos taurus(cattle)

The following EDC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09354 XM_002694902.6
Latest version!
Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa09354 XM_001788092.5
Latest version!
Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa09355 XM_015475827.2
Latest version!
Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa09355 XM_015467380.1
Latest version!
Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBa09354 XM_002694902.5 Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa09355 XM_015475827.1 Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09354
    Clone ID Related Accession (Same CDS sequence) XM_002694902.5 , XM_001788092.5 , XM_002694902.6
    Accession Version XM_002694902.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694902.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694948.2
    CDS199..4401
    Translation

    Target ORF information:

    RefSeq Version XM_002694902.5
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694902.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09354
    Clone ID Related Accession (Same CDS sequence) XM_002694902.5 , XM_001788092.5 , XM_002694902.6
    Accession Version XM_001788092.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001788092.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001788144.2
    CDS199..4401
    Translation

    Target ORF information:

    RefSeq Version XM_001788092.5
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001788092.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09354
    Clone ID Related Accession (Same CDS sequence) XM_002694902.5 , XM_001788092.5 , XM_002694902.6
    Accession Version XM_002694902.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694902.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694948.2
    CDS206..4408
    Translation

    Target ORF information:

    RefSeq Version XM_002694902.6
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694902.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCCT CTTGTGCGAG CATCGACATA GAGGACGCCA CGCAGCACCT GCGGGACATC 
    CTCAAGCTGG ACCGGCCGGC GGGAGGCCCC AGTGCAGAGA ACCAACGACC ATCTAATGCC
    TACAATGGAG ATCTCAATGG GCTCCTGGTC CCAGACCCCC TCTGCTCAGG TGACAGTGCT
    TCAACAAACA AGCCTGGTTT CCGAGCCATG CCACCTATTA ACCTGCAGGA AAAGCAGGTC
    ATCTGCCTCT TAGGAGACGA CAGTTCCACT GGCATTGGAA TTTTGGCCAA GGAGGTGGAG
    ATTGTGGCCA GCAGTGACTC TAGCATCTCG AGCAAGGCAC GGGGGAGCAA CAAGGTGAAA
    ATTCAGCCTG TCGCCAAGTA TGACTGGGAG CAGAAATACT ACTATGGCAA CTTGATTGCT
    GTGTCCAACT CTTTCTTGGC CTATGCTATT CGGGCTGCCA ACAACGGCTC AGCAATGGTG
    CGAGTGATCA GTGTCAGCAC TTCAGAGCGG ACCCTGCTCA AAGGCTTCAC AGGCAGCGTG
    GCTGATCTGG CCTTTGCACA CCTCAATTCC CCACAGCTGG CCTGCCTGGA TGAGGCAGGC
    AACCTGTTTG TATGGCGCTT GGCCCTGATT AATGGCAAAA TTCAAGAGGA GATCTTGGTC
    CACATCCGCC AGCCAGAGGG CACTCCGCTG AACCACTTCC GCAGGATCAT CTGGTGCCCC
    TTCATCCCTG AAGAGAGTGA GGACTGCTGT GAGGAGGGCA GCCCAACGGT GGCCCTGTTG
    CATGAGGACC GGGCTGAGGT GTGGGACCTG GACATGCTCC GCACCAACCA CAGCACCTGG
    CCTGTGGATG TCAGCCAGAT CAAGCAGGGC TTCATCGTGG TAAAAGGCCA CAGCACATGC
    TTGAGTGAAG GGGCCCTCTC ACCTGATGGG ACTGTCCTGG CTACTGCAAG TCATGATGGC
    TTCGTCAAGT TCTGGCAGAT CTACATCGAG GGACAGGATG AGCCACGGTG TTTACATGAG
    TGGAAGCCTC ATGATGGGCG GCCCCTCTCC TGCCTCCTGT TCTGTGACAA CCACAAGAAA
    CAGGATCCTG AGGTCCCTTT CTGGAGATTC CTCATTACTG GCGCTGACCA GAATCGGGAG
    CTGAAGATGT GGTGCACGGT GTCCTGGACC TGCCTGCAGA CCATTCGCTT CTCCCCAGAT
    ATCTTCAGCC CAGTGAGTGT GCCCCCCAGC CTCAAGGTTT GCCTGGACCT CTCAGCGGAA
    TACCTGATCC TCAGTGATGT ACAGCGGAAG GTCCTCTACG TGATGGAACT GCTGCAGAAC
    CAGGAGGAGG GCCGGGCCTG CTTCAGCTCC ATCTCTGAGT TCCTTCTAAC CCACCCTGTA
    CTGAGCTTCG GTATCCAGGT TGTGAGTCGC TGCCGGCTGC GGCACACAGA GGTGCTGCCT
    GCCGAGGAGG AGAATGACAG CCTTGGGGCC GACGGGACCC ATGGAGCAGG TGCAATGGAG
    TCTGCAGCCG GTGTGCTCAT CAAGCTCTTT TGTGTGCACA CTAAGGCATT ACAGGACGTC
    CAGATCCGCT TTCAGCCACA GCTGAACCCT GACGTTGTGG CCCCACTCCC CTCGCACTCT
    ACCCATGAAG ACTTTCCATT TGGAGAGTCT CGGCCAGAGC TGGGCTCAGA GGGCCTGGGA
    TCAGCTACTC ATGGCTCCCA GCCTGACCTC CGACGTATCG TGGAGTTGCC TGCACCAGCT
    GACTTCCTCA GTCTGAGCAG TGAGACCAAG CCCAAGCTGA TGACACCTGA CGCCTTCATG
    ACGCCTAGCG CCTCCCTGCA GCAGATTGCT GCATCTCCCA GCAGCAGCAG CAGCAGCAGC
    AGCAGTAGCA GCAGCAGCAG CTCCTCTCTG ACAGCCGTGT CTGCCATGAG CAGTACTTCG
    GCCGTGGACC CCTCCTTGCC CAGGCCACCT GAGGAGCTGA CCTTGAGCCC CAAGCTACAG
    CTGGATGGTG GTCTGACCAT GAGCAGCAGC AGCCTGCAGG CAAGCCCACG TGGGCTCCTG
    CCCAGCCTGC TCCCAGGTCC GGCTGACAAA CTGACTCCTA AAGGGTCTGG TCAGGTGCCT
    ACTGCTGCCT CTGCACTCGC CCTGGAGCTG CAGGAAGTGG AACCCCTGGG GCTGCCTCAG
    GCTTCCCCCA GCCGCACCCG CTCCCCCGAT GTTATCTCCT CAGCCTCCAC TGCCTTGTCC
    CAGGACATCC CTGAGATTGC ATCTGAGGCG CTGTCCCGTG GCTTTGGCGC CTCTGCTCCT
    GAGGGCCTGG AGCCAGACAG CATGGCCTCA GCGGCCTCGG CGCTCCACCT GTTGTCTCCA
    CGGCCCCGGC CAGGGCCCGA GCTCGGCCCC CAGCTTAGCC TGGATGGGGT CCCTGGGGAT
    GGGGATCGGC ATAGTACCCC TTCCCTCCTG GAGGCAGCCT TGACCCAGGA GGCCACAGCC
    CCTGACAGTC AGGTCTGGCC TACAGCTCCA GACATCACTC GCGAGACCTG CAGCAGCCTG
    GCAGAGAGCC CCCGGAATGG CCTCCAGGAA AAGCACAAGA GCCTGGCCTT CCACCGACCA
    CCTTATCACC TGCTGCAGCA ACACGACAGC CAGGACGCCA GTGCCGAGCA AAGTGACCAT
    GATGACGAGG TGGCCAGCCT CGCCTCTGCT GCAGGGAACT TTGGCACCAA AGTGCCCACT
    CCACGTCTTC CAGCCAAGGA CTGGAAGACC AAGGGATCCC CTCGGGCCTC ACCCAAGCTC
    AAGAGAAAGG GCAAAAAGGA TGATGGGGAT TCATCCTTGG GATCCCGGCT CACAGAGCAC
    CAGGTGGCAG AAACCCCTGA GGACTGGCCG GCACTGATCT GGCAGCAGCA GAGAGAGCTG
    GCACAGCTGC GGCACAGCCA AGAAGAGCTG CTGCAGCGCC TGTGCGCCCA GCTTGAAGGC
    CTGCAGAGCA CCGTCACAGG CCACGTGGAG CGAGCCCTGG AGTCAAGGCA CGAGCAGGAG
    CAGCGGCGAC TGGAGCGGGC CCTGGCCGAG GGGCAGCAGC GGGGTGGGCA GCTGCAGGAG
    CAGCTGACAC AGCAGCTATC CCAGGCGCTG TCTTCAGCGG TGGCAGGGCG GCTGGAGCGC
    AGCGTACGAG ACGAGATCAA GAAGACGGTT CCTCCATGTG TCTCCCGGAG TCTGGAGCCC
    GTGGCTGGCC AGCTCAGCAA CTCGGTGGCC ACCAAACTCA CAGCCGTGGA GAGTAGCATG
    AAAGAGAACA TCTCTAAGCT GCTCAAGTCC AAGAACTTGA CAGATGCCAT TGCCCGAGCA
    GCCGCAGACA CATTACAGGG GCCAATGCAG GCCGCCTACC GGGAAGCCTT CCAGAGTGTG
    GTCCTGCCCG CCTTCGAGAA GAGCTGCCAG GCCATGTTCC AGCAGATCAA TGACAGCTTC
    CGACTGGGCA CACAGGAGTA CTTGCAGCAG TTGGAGAGCC ACATGAAGAG CCGCAAGGCA
    CGAGAGCAGG AGGCACGGGA GCCTGTGCTG GCCCAGCTTC GGGGCCTGGT CGGCACCCTG
    CAGGGGGCCA CGGAGCAGAT GGCGGCCACT GTGTCTGGCA GCGTTCGGGC TGAGGTGCAG
    CACCAGCTGC ATGTGGCCGT GGGCAGCCTG CAGGAGGCGA TTCTAGCACA GGTACAGCGC
    ATCGTGAAGG GCGAGGTGAG TGTGGCTCTC AAGGAGCAGC AGGCCGCCGT CACCTCTAGC
    ATCATGCAGG CCATGCGCTC AGCCGCCGGC ACACCTGTCC CTGCTGCCCA CCTCGACTGC
    CAAGCCCAGC AAGCCCATAT CCTGCAGCTG CTGCAGCAGG GCCACCTCAA TCAGGCCTTC
    CAGCAGGCCC TGACAGCTGC CGACCTGAAC CTGGTGCTAT ACGTGTGTGA AACAGTGGAC
    CCAGGCCAGG TTTTTGGGCA GCCACCTTGC CCCCTCTCCC AGCCTGTACT CCTTTCTCTC
    ATCCAGCAAC TGGCCTCTGA TCTTGGCACT CGAACTGACC TCAAGCTCAG CTACCTGGAA
    GAGGCTGTAA TGCACCTGGA CCACAGTGAC CCCATCACTC GGGACCACAT GGGCTCCGTC
    ATGGCTCAGG TGCGCCAGAA GCTCTTCCAG TTCCTGCAGG CGGAGCCACA CAACTCACTT
    GGCAAAGCAG CCCGGCGTCT CAGCCTCATG CTGCACGGCC TTATGACCCC CAGCCTCCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09355
    Clone ID Related Accession (Same CDS sequence) XM_015467380.1 , XM_015475827.1 , XM_015475827.2
    Accession Version XM_015467380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322866.1
    CDS539..4534
    Translation

    Target ORF information:

    RefSeq Version XM_015467380.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09355
    Clone ID Related Accession (Same CDS sequence) XM_015467380.1 , XM_015475827.1 , XM_015475827.2
    Accession Version XM_015475827.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331313.1
    CDS539..4534
    Translation

    Target ORF information:

    RefSeq Version XM_015475827.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09355
    Clone ID Related Accession (Same CDS sequence) XM_015467380.1 , XM_015475827.1 , XM_015475827.2
    Accession Version XM_015475827.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product enhancer of mRNA-decapping protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331313.1
    CDS527..4522
    Translation

    Target ORF information:

    RefSeq Version XM_015475827.2
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of mRNA decapping 4 (EDC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475827.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCACCTA TTAACCTGCA GGAAAAGCAG GTCATCTGCC TCTTAGGAGA CGACAGTTCC 
    ACTGGCATTG GAATTTTGGC CAAGGAGGTG GAGATTGTGG CCAGCAGTGA CTCTAGCATC
    TCGAGCAAGG CACGGGGGAG CAACAAGGTG AAAATTCAGC CTGTCGCCAA GTATGACTGG
    GAGCAGAAAT ACTACTATGG CAACTTGATT GCTGTGTCCA ACTCTTTCTT GGCCTATGCT
    ATTCGGGCTG CCAACAACGG CTCAGCAATG GTGCGAGTGA TCAGTGTCAG CACTTCAGAG
    CGGACCCTGC TCAAAGGCTT CACAGGCAGC GTGGCTGATC TGGCCTTTGC ACACCTCAAT
    TCCCCACAGC TGGCCTGCCT GGATGAGGCA GGCAACCTGT TTGTATGGCG CTTGGCCCTG
    ATTAATGGCA AAATTCAAGA GGAGATCTTG GTCCACATCC GCCAGCCAGA GGGCACTCCG
    CTGAACCACT TCCGCAGGAT CATCTGGTGC CCCTTCATCC CTGAAGAGAG TGAGGACTGC
    TGTGAGGAGG GCAGCCCAAC GGTGGCCCTG TTGCATGAGG ACCGGGCTGA GGTGTGGGAC
    CTGGACATGC TCCGCACCAA CCACAGCACC TGGCCTGTGG ATGTCAGCCA GATCAAGCAG
    GGCTTCATCG TGGTAAAAGG CCACAGCACA TGCTTGAGTG AAGGGGCCCT CTCACCTGAT
    GGGACTGTCC TGGCTACTGC AAGTCATGAT GGCTTCGTCA AGTTCTGGCA GATCTACATC
    GAGGGACAGG ATGAGCCACG GTGTTTACAT GAGTGGAAGC CTCATGATGG GCGGCCCCTC
    TCCTGCCTCC TGTTCTGTGA CAACCACAAG AAACAGGATC CTGAGGTCCC TTTCTGGAGA
    TTCCTCATTA CTGGCGCTGA CCAGAATCGG GAGCTGAAGA TGTGGTGCAC GGTGTCCTGG
    ACCTGCCTGC AGACCATTCG CTTCTCCCCA GATATCTTCA GCCCAGTGAG TGTGCCCCCC
    AGCCTCAAGG TTTGCCTGGA CCTCTCAGCG GAATACCTGA TCCTCAGTGA TGTACAGCGG
    AAGGTCCTCT ACGTGATGGA ACTGCTGCAG AACCAGGAGG AGGGCCGGGC CTGCTTCAGC
    TCCATCTCTG AGTTCCTTCT AACCCACCCT GTACTGAGCT TCGGTATCCA GGTTGTGAGT
    CGCTGCCGGC TGCGGCACAC AGAGGTGCTG CCTGCCGAGG AGGAGAATGA CAGCCTTGGG
    GCCGACGGGA CCCATGGAGC AGGTGCAATG GAGTCTGCAG CCGGTGTGCT CATCAAGCTC
    TTTTGTGTGC ACACTAAGGC ATTACAGGAC GTCCAGATCC GCTTTCAGCC ACAGCTGAAC
    CCTGACGTTG TGGCCCCACT CCCCTCGCAC TCTACCCATG AAGACTTTCC ATTTGGAGAG
    TCTCGGCCAG AGCTGGGCTC AGAGGGCCTG GGATCAGCTA CTCATGGCTC CCAGCCTGAC
    CTCCGACGTA TCGTGGAGTT GCCTGCACCA GCTGACTTCC TCAGTCTGAG CAGTGAGACC
    AAGCCCAAGC TGATGACACC TGACGCCTTC ATGACGCCTA GCGCCTCCCT GCAGCAGATT
    GCTGCATCTC CCAGCAGCAG CAGCAGCAGC AGCAGCAGTA GCAGCAGCAG CAGCTCCTCT
    CTGACAGCCG TGTCTGCCAT GAGCAGTACT TCGGCCGTGG ACCCCTCCTT GCCCAGGCCA
    CCTGAGGAGC TGACCTTGAG CCCCAAGCTA CAGCTGGATG GTGGTCTGAC CATGAGCAGC
    AGCAGCCTGC AGGCAAGCCC ACGTGGGCTC CTGCCCAGCC TGCTCCCAGG TCCGGCTGAC
    AAACTGACTC CTAAAGGGTC TGGTCAGGTG CCTACTGCTG CCTCTGCACT CGCCCTGGAG
    CTGCAGGAAG TGGAACCCCT GGGGCTGCCT CAGGCTTCCC CCAGCCGCAC CCGCTCCCCC
    GATGTTATCT CCTCAGCCTC CACTGCCTTG TCCCAGGACA TCCCTGAGAT TGCATCTGAG
    GCGCTGTCCC GTGGCTTTGG CGCCTCTGCT CCTGAGGGCC TGGAGCCAGA CAGCATGGCC
    TCAGCGGCCT CGGCGCTCCA CCTGTTGTCT CCACGGCCCC GGCCAGGGCC CGAGCTCGGC
    CCCCAGCTTA GCCTGGATGG GGTCCCTGGG GATGGGGATC GGCATAGTAC CCCTTCCCTC
    CTGGAGGCAG CCTTGACCCA GGAGGCCACA GCCCCTGACA GTCAGGTCTG GCCTACAGCT
    CCAGACATCA CTCGCGAGAC CTGCAGCAGC CTGGCAGAGA GCCCCCGGAA TGGCCTCCAG
    GAAAAGCACA AGAGCCTGGC CTTCCACCGA CCACCTTATC ACCTGCTGCA GCAACACGAC
    AGCCAGGACG CCAGTGCCGA GCAAAGTGAC CATGATGACG AGGTGGCCAG CCTCGCCTCT
    GCTGCAGGGA ACTTTGGCAC CAAAGTGCCC ACTCCACGTC TTCCAGCCAA GGACTGGAAG
    ACCAAGGGAT CCCCTCGGGC CTCACCCAAG CTCAAGAGAA AGGGCAAAAA GGATGATGGG
    GATTCATCCT TGGGATCCCG GCTCACAGAG CACCAGGTGG CAGAAACCCC TGAGGACTGG
    CCGGCACTGA TCTGGCAGCA GCAGAGAGAG CTGGCACAGC TGCGGCACAG CCAAGAAGAG
    CTGCTGCAGC GCCTGTGCGC CCAGCTTGAA GGCCTGCAGA GCACCGTCAC AGGCCACGTG
    GAGCGAGCCC TGGAGTCAAG GCACGAGCAG GAGCAGCGGC GACTGGAGCG GGCCCTGGCC
    GAGGGGCAGC AGCGGGGTGG GCAGCTGCAG GAGCAGCTGA CACAGCAGCT ATCCCAGGCG
    CTGTCTTCAG CGGTGGCAGG GCGGCTGGAG CGCAGCGTAC GAGACGAGAT CAAGAAGACG
    GTTCCTCCAT GTGTCTCCCG GAGTCTGGAG CCCGTGGCTG GCCAGCTCAG CAACTCGGTG
    GCCACCAAAC TCACAGCCGT GGAGAGTAGC ATGAAAGAGA ACATCTCTAA GCTGCTCAAG
    TCCAAGAACT TGACAGATGC CATTGCCCGA GCAGCCGCAG ACACATTACA GGGGCCAATG
    CAGGCCGCCT ACCGGGAAGC CTTCCAGAGT GTGGTCCTGC CCGCCTTCGA GAAGAGCTGC
    CAGGCCATGT TCCAGCAGAT CAATGACAGC TTCCGACTGG GCACACAGGA GTACTTGCAG
    CAGTTGGAGA GCCACATGAA GAGCCGCAAG GCACGAGAGC AGGAGGCACG GGAGCCTGTG
    CTGGCCCAGC TTCGGGGCCT GGTCGGCACC CTGCAGGGGG CCACGGAGCA GATGGCGGCC
    ACTGTGTCTG GCAGCGTTCG GGCTGAGGTG CAGCACCAGC TGCATGTGGC CGTGGGCAGC
    CTGCAGGAGG CGATTCTAGC ACAGGTACAG CGCATCGTGA AGGGCGAGGT GAGTGTGGCT
    CTCAAGGAGC AGCAGGCCGC CGTCACCTCT AGCATCATGC AGGCCATGCG CTCAGCCGCC
    GGCACACCTG TCCCTGCTGC CCACCTCGAC TGCCAAGCCC AGCAAGCCCA TATCCTGCAG
    CTGCTGCAGC AGGGCCACCT CAATCAGGCC TTCCAGCAGG CCCTGACAGC TGCCGACCTG
    AACCTGGTGC TATACGTGTG TGAAACAGTG GACCCAGGCC AGGTTTTTGG GCAGCCACCT
    TGCCCCCTCT CCCAGCCTGT ACTCCTTTCT CTCATCCAGC AACTGGCCTC TGATCTTGGC
    ACTCGAACTG ACCTCAAGCT CAGCTACCTG GAAGAGGCTG TAATGCACCT GGACCACAGT
    GACCCCATCA CTCGGGACCA CATGGGCTCC GTCATGGCTC AGGTGCGCCA GAAGCTCTTC
    CAGTTCCTGC AGGCGGAGCC ACACAACTCA CTTGGCAAAG CAGCCCGGCG TCTCAGCCTC
    ATGCTGCACG GCCTTATGAC CCCCAGCCTC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.