ATN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATN1
Entrez Gene ID	513125
Full Name	atrophin 1
General protein information	Preferred Names atrophin 1 Names atrophin-1 arginine-glutamic acid dipeptide (RE) repeats
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024991648.1	XP_024847416.1	atrophin-1 isoform X1
XM_024991649.1	XP_024847417.1	atrophin-1 isoform X1
XM_005207134.4	XP_005207191.1	atrophin-1 isoform X2
XM_005207132.4	XP_005207189.1	atrophin-1 isoform X1
NM_001205748.2	NP_001192677.1	atrophin-1
NM_001205748.1	NP_001192677.1	atrophin-1
XM_005207132.3	XP_005207189.1	atrophin-1 isoform X1
XM_005207134.3	XP_005207191.1	atrophin-1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atn1	NP_031907.2
Gallus gallus (chicken)	ATN1	XP_425518.4
Canis lupus familiaris (dog)	ATN1	XP_003640006.1
Bos taurus (cattle)	ATN1	NP_001192677.1
Rattus norvegicus (Norway rat)	LOC100911672	XP_003750706.1
Pan troglodytes (chimpanzee)	ATN1	NP_001029337.1
Rattus norvegicus (Norway rat)	Atn1	NP_058924.2
Homo sapiens (human)	ATN1	NP_001007027.1
	ATN1	XP_001118307.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ATN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234322	NM_001205748.2 Latest version!	Bos taurus atrophin 1 (ATN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa234322	XM_024991649.1 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa234322	XM_024991648.1 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa234322	XM_005207132.4 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa234323	XM_005207134.4 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa19790	NM_001205748.1 Latest version!	Bos taurus atrophin 1 (ATN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa19790	XM_005207132.3 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa19791	XM_005207134.3 Latest version!	Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATN1 ( NM_001205748.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192677.1 Bos taurus atrophin 1 (ATN1), mRNA.

Price & Availability↑

CloneID	OBa234322
Clone ID Related Accession (Same CDS sequence)	XM_024991648.1 , XM_024991649.1 , XM_005207132.4 , NM_001205748.2
Accession Version	NM_001205748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	atrophin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On May 29, 2018 this sequence version replaced NM_001205748.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192677.1
CDS	351..3905
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	NM_001205748.2
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205748.2

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_024991648.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847416.1 Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa234322
Clone ID Related Accession (Same CDS sequence)	XM_024991648.1 , XM_024991649.1 , XM_005207132.4 , NM_001205748.2
Accession Version	XM_024991648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847416.1
CDS	501..4055
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_024991648.1
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991648.1

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_024991649.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847417.1 Bos taurus atrophin 1 (ATN1), transcript variant X3, mRNA.

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CloneID	OBa234322
Clone ID Related Accession (Same CDS sequence)	XM_024991648.1 , XM_024991649.1 , XM_005207132.4 , NM_001205748.2
Accession Version	XM_024991649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847417.1
CDS	248..3802
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_024991649.1
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991649.1

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_005207132.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207189.1 Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.

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CloneID	OBa234322
Clone ID Related Accession (Same CDS sequence)	XM_024991648.1 , XM_024991649.1 , XM_005207132.4 , NM_001205748.2
Accession Version	XM_005207132.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207132.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207189.1
CDS	314..3868
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_005207132.4
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207132.4

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAG 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_005207134.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207191.1 Bos taurus atrophin 1 (ATN1), transcript variant X4, mRNA.

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CloneID	OBa234323
Clone ID Related Accession (Same CDS sequence)	XM_005207134.4
Accession Version	XM_005207134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGG AGCTGCCCCG GCCCCAGTCT 
CCCTCCGATC TGGACAGTCT GGATGGGCGG AGCCTCAATG ACGATGGCAG CAGTGACCCC 
AGGGACATCG ACCAGGACAA CCGGAGCACG TCTCCTAGCA TCTACAGCCC TGGAAGCGTG 
GAGAATGACT CCGACTCGTC TTCTGGCCTG TCCCAGGGCC CAGCCCGCCC CTATCACCCA 
CCTCCACTCT TCCCTCCCTC CCCTCCACCA CCTGACAGCA CCCCCCGACA GCCAGAGCCT 
GGCTTTGAAC CCCACCCTTC TGTGACACCC ACCGGATATC ATGCTCCCAT GGAGCCCCCC 
ACATCTCGGA TGTTCCAGGC TCCTCCAGGG GCTCCTCCCC CTCATCCACA GCTCTACCCT 
GGGGGCTCTG GTGGGGGAGT TTTGTCTGGA CCCCCACTGG GTCCCAAGGG GAGTGGGGCT 
GCCTCTTCAG TGGGGGCCCC TAGTGGGGGT AAGCAGCACC CCCCACCCAC CACCCCCATT 
TCAATATCAA GCTCTGGGGC TGGTGGTGCT CCCCCAACGA AGCCACCTAA CACTCCAGTC 
GGTGGTGGAA GCCTACCGTC TGCGCCACCA CCAGCCTCCT TCCCCCACGT GACACCAAAC 
CTGCCTCCCC CACCTGCTCT GCGACCCCTT AACAATGCAT CGGCCTCTCC TCCTGGCCTG 
GGGGCCCAGC CACTACCTGG GCATCTCCCT TCCCCCCATG CCATGGGGCA GAGCATGGGT 
GGACTTCCTC CTGGCCCGGA GAAGGGGCCC ACTCTGGCTC CTTCACCCCA TCCTCTGCCC 
CCTGCGTCCT CTTCAGCCCC TGCCCCCACA ATGAGGTATC CTTACTCATC CTCTACTAGC 
AGCTCTGCAG CAGCCGCCTC TTCCAATTCT TCTGCCTCTG CCTCTGCCTC CCAGTACCCC 
GCTTCCCAGG CACTGCCCAG TTATCCCCAC TCCTTCCCTC CCCCGACCAG TCTCTCTGTC 
TCTAATCAGC CCCCCAAGTA TACGCAGCCT TCTCTTCCAT CCCAGGCTGT GTGGAGCCAG 
GGTCCCCCTC CACCTCCTCC CTATGGCCGC CTCTTAGCCA ACAGCAGTGC CCACCCAGGT 
CCCTTCCCTC CTTCAGCTGG AGGCCAGTCC ACAGCCCACC CATCAGCCCC AACACATCAC 
CACCACCACC AGCAACAGCA GCAGCAGCAG CAGCAACCAC CACCACCACA TCATGGGGGC 
TCTGGGCCCC CTCCCCCTGG AGCATATCCT CACCCCCTGG AGAGCGGTGG TTCCCACCAT 
GCACACCCCT ATGCCATGTC TCCCTCCCTG GGGTCTCTGA GACCCTATCC ACCAGGGCCA 
GCACACCTGC CCCCACCTCA CAGCCAGGTG TCCTACAGCC AAGCAGGTCC TAATGGACCC 
CCAACCTCCT CTTCTTCCAA TTCCTCTTCC TCCTCTCAAG GGTCCTACCC GGGTTCACAC 
CCCTCTCCTT CCCAGGGCCC CTCAGGGGCG CCCTACCCCT TTCCACCGGT GCCTCCGGTC 
ACCACTTCCT CGGCCACACT TTCCACGGTC ATTGCCACAG TGGCTTCCTC GCCAGCAGGC 
TACAAGACAG CCTCCCCACC TGGGCCCCCG CCGTATGGCA AGCGAGCCCC GTCCCCAGGG 
GCCTACAAGA CAGCCACTCC ACCTGGATAC AAGCCGGGGT CGCCGCCCTC CTTCCGAACG 
GGGACCCCAC CGGGCTACCG AGGCGCCTCG CCGCCCGCAG GCCCAGGGAC CTTCAAGCCG 
GGGTCGCCCA CCGTGGGGCC AGGGCCGCTG CCGCCTGCGG GGCCCTCCGG CCTGTCGTCC 
CTGCCCGCAC CGCCTGCGGC CCCTGCCTCT GGGCCGCCCC TGAGTGCCAC GCAGATCAAA 
CAGGAGCCGG CGGAGGAATA TGAGACCCCC GAGAGCCCGG TGCCCCCGGC CCGCAGCCCC 
TCGCCCCCTC CCAAGGTGGT AGATGTGCCC AGCCACGCCA GCCAGTCGGC CAGGTTCAAC 
AAACACCTGG ACCGCGGCTT TAACTCGTGC GCGCGCAGCG ATCTGTACTT CGTACCGCTG 
GAGGGGTCCA AGCTGGCCAA GAAACGGGCC GACCTGGTAG AGAAGGTGCG GCGCGAGGCC 
GAACAGCGCG CGCGCGAAGA AAAGGAGCGC GAGCGCGAGC GGGAGCGCGA GAAGGAGCGC 
GAGCGCGAGA AGGAGCGCGA GCTGGAACGC AGCGTGAAGT TGGCTCAGGA GGGCCGTGCT 
CCAGTGGAGT GCCCATCTCT CGGCCCAGTG CCCCATCGCC CTCCGTTTGA GCCGGGCAGT 
GCTGTGGCTA CAGTGCCCCC TTACCTGGGC CCCGACACTC CAGCCCTACG CACCCTCAGT 
GAATACGCCC GGCCCCACGT GATGTCTCCT GGCAATCGCA ACCATCCGTT CTACGTGCCC 
CTGGGGGCAG TGGACCCGGG GCTCCTGGGT TACAATGTCC CGGCCTTGTA CAGCAGTGAC 
CCAGCAGCCC GGGAGAGGGA GCGGGAAGCC CGTGAACGAG ACCTGCGTGA CCGCCTCAAG 
CCTGGCTTTG AGGTGAAGCC CAGCGAGCTG GAACCCCTAC ATGGGGTCCC TGGGCCAGGC 
CTGGATCCCT TCCCCCGACA CGGGGGCCTG GCTTTGCAGC CTGGCCCCCC GGGCTTGCAC 
CCTTTCCCCT TCCATCCGAG CCTGGGGCCG CTGGAGAGAG AACGTCTAGC TCTGGCAGCT 
GGGCCCGCCC TGCGGCCTGA CATGTCCTAC GCGGAGCGTC TGGCAGCTGA GAGGCAGCAT 
GCAGAAAGGG TGGCCGCCCT GGGCAATGAC CCACTGGCGC GGCTGCAGAT GCTCAACGTG 
ACCCCCCATC ACCACCAGCA CTCCCACATC CATTCTCACC TGCACCTCCA CCAGCAGGAT 
GCCATCCACG CAGCCTCTGC CTCGGTGCAC CCTCTCATTG ATCCCCTGGC CTCAGGGTCT 
CACCTTACCC GGATCCCCTA CCCAGCTGGG ACCCTCCCCA ACCCCCTTCT TCCTCACCCT 
CTGCACGAGA ACGAAGTTCT TCGTCACCAG CTCTTTGCTG CTCCATACCG GGACCTGCCA 
GCCTCCCTCT CTGCCCCGAT GTCGGCAGCT CACCAGCTGC AGGCCATGCA CGCACAGTCA 
GCTGAGTTGC AGCGCTTGGC GCTGGAGCAG CAGCAGTGGC TGCACGCCCA TCACCCGCTG 
CACAGTGTGC CGCTGCCCGC CCAGGAGGAC TACTACAGTC ATCTGAAGAA GGAAAGCGAC 
AAGCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207191.1
CDS	314..3865
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_005207134.4
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207134.4

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGG AGCTGCCCCG GCCCCAGTCT 
CCCTCCGATC TGGACAGTCT GGATGGGCGG AGCCTCAATG ACGATGGCAG CAGTGACCCC 
AGGGACATCG ACCAGGACAA CCGGAGCACG TCTCCTAGCA TCTACAGCCC TGGAAGCGTG 
GAGAATGACT CCGACTCGTC TTCTGGCCTG TCCCAGGGCC CAGCCCGCCC CTATCACCCA 
CCTCCACTCT TCCCTCCCTC CCCTCCACCA CCTGACAGCA CCCCCCGACA GCCAGAGCCT 
GGCTTTGAAC CCCACCCTTC TGTGACACCC ACCGGATATC ATGCTCCCAT GGAGCCCCCC 
ACATCTCGGA TGTTCCAGGC TCCTCCAGGG GCTCCTCCCC CTCATCCACA GCTCTACCCT 
GGGGGCTCTG GTGGGGGAGT TTTGTCTGGA CCCCCACTGG GTCCCAAGGG GAGTGGGGCT 
GCCTCTTCAG TGGGGGCCCC TAGTGGGGGT AAGCAGCACC CCCCACCCAC CACCCCCATT 
TCAATATCAA GCTCTGGGGC TGGTGGTGCT CCCCCAACGA AGCCACCTAA CACTCCAGTC 
GGTGGTGGAA GCCTACCGTC TGCGCCACCA CCAGCCTCCT TCCCCCACGT GACACCAAAC 
CTGCCTCCCC CACCTGCTCT GCGACCCCTT AACAATGCAT CGGCCTCTCC TCCTGGCCTG 
GGGGCCCAGC CACTACCTGG GCATCTCCCT TCCCCCCATG CCATGGGGCA GAGCATGGGT 
GGACTTCCTC CTGGCCCGGA GAAGGGGCCC ACTCTGGCTC CTTCACCCCA TCCTCTGCCC 
CCTGCGTCCT CTTCAGCCCC TGCCCCCACA ATGAGGTATC CTTACTCATC CTCTACTAGC 
AGCTCTGCAG CAGCCGCCTC TTCCAATTCT TCTGCCTCTG CCTCTGCCTC CCAGTACCCC 
GCTTCCCAGG CACTGCCCAG TTATCCCCAC TCCTTCCCTC CCCCGACCAG TCTCTCTGTC 
TCTAATCAGC CCCCCAAGTA TACGCAGCCT TCTCTTCCAT CCCAGGCTGT GTGGAGCCAG 
GGTCCCCCTC CACCTCCTCC CTATGGCCGC CTCTTAGCCA ACAGCAGTGC CCACCCAGGT 
CCCTTCCCTC CTTCAGCTGG AGGCCAGTCC ACAGCCCACC CATCAGCCCC AACACATCAC 
CACCACCACC AGCAACAGCA GCAGCAGCAG CAGCAACCAC CACCACCACA TCATGGGGGC 
TCTGGGCCCC CTCCCCCTGG AGCATATCCT CACCCCCTGG AGAGCGGTGG TTCCCACCAT 
GCACACCCCT ATGCCATGTC TCCCTCCCTG GGGTCTCTGA GACCCTATCC ACCAGGGCCA 
GCACACCTGC CCCCACCTCA CAGCCAGGTG TCCTACAGCC AAGCAGGTCC TAATGGACCC 
CCAACCTCCT CTTCTTCCAA TTCCTCTTCC TCCTCTCAAG GGTCCTACCC GGGTTCACAC 
CCCTCTCCTT CCCAGGGCCC CTCAGGGGCG CCCTACCCCT TTCCACCGGT GCCTCCGGTC 
ACCACTTCCT CGGCCACACT TTCCACGGTC ATTGCCACAG TGGCTTCCTC GCCAGCAGGC 
TACAAGACAG CCTCCCCACC TGGGCCCCCG CCGTATGGCA AGCGAGCCCC GTCCCCAGGG 
GCCTACAAGA CAGCCACTCC ACCTGGATAC AAGCCGGGGT CGCCGCCCTC CTTCCGAACG 
GGGACCCCAC CGGGCTACCG AGGCGCCTCG CCGCCCGCAG GCCCAGGGAC CTTCAAGCCG 
GGGTCGCCCA CCGTGGGGCC AGGGCCGCTG CCGCCTGCGG GGCCCTCCGG CCTGTCGTCC 
CTGCCCGCAC CGCCTGCGGC CCCTGCCTCT GGGCCGCCCC TGAGTGCCAC GCAGATCAAA 
CAGGAGCCGG CGGAGGAATA TGAGACCCCC GAGAGCCCGG TGCCCCCGGC CCGCAGCCCC 
TCGCCCCCTC CCAAGGTGGT AGATGTGCCC AGCCACGCCA GCCAGTCGGC CAGGTTCAAC 
AAACACCTGG ACCGCGGCTT TAACTCGTGC GCGCGCAGCG ATCTGTACTT CGTACCGCTG 
GAGGGGTCCA AGCTGGCCAA GAAACGGGCC GACCTGGTAG AGAAGGTGCG GCGCGAGGCC 
GAACAGCGCG CGCGCGAAGA AAAGGAGCGC GAGCGCGAGC GGGAGCGCGA GAAGGAGCGC 
GAGCGCGAGA AGGAGCGCGA GCTGGAACGC AGCGTGAAGT TGGCTCAGGA GGGCCGTGCT 
CCAGTGGAGT GCCCATCTCT CGGCCCAGTG CCCCATCGCC CTCCGTTTGA GCCGGGCAGT 
GCTGTGGCTA CAGTGCCCCC TTACCTGGGC CCCGACACTC CAGCCCTACG CACCCTCAGT 
GAATACGCCC GGCCCCACGT GATGTCTCCT GGCAATCGCA ACCATCCGTT CTACGTGCCC 
CTGGGGGCAG TGGACCCGGG GCTCCTGGGT TACAATGTCC CGGCCTTGTA CAGCAGTGAC 
CCAGCAGCCC GGGAGAGGGA GCGGGAAGCC CGTGAACGAG ACCTGCGTGA CCGCCTCAAG 
CCTGGCTTTG AGGTGAAGCC CAGCGAGCTG GAACCCCTAC ATGGGGTCCC TGGGCCAGGC 
CTGGATCCCT TCCCCCGACA CGGGGGCCTG GCTTTGCAGC CTGGCCCCCC GGGCTTGCAC 
CCTTTCCCCT TCCATCCGAG CCTGGGGCCG CTGGAGAGAG AACGTCTAGC TCTGGCAGCT 
GGGCCCGCCC TGCGGCCTGA CATGTCCTAC GCGGAGCGTC TGGCAGCTGA GAGGCAGCAT 
GCAGAAAGGG TGGCCGCCCT GGGCAATGAC CCACTGGCGC GGCTGCAGAT GCTCAACGTG 
ACCCCCCATC ACCACCAGCA CTCCCACATC CATTCTCACC TGCACCTCCA CCAGCAGGAT 
GCCATCCACG CAGCCTCTGC CTCGGTGCAC CCTCTCATTG ATCCCCTGGC CTCAGGGTCT 
CACCTTACCC GGATCCCCTA CCCAGCTGGG ACCCTCCCCA ACCCCCTTCT TCCTCACCCT 
CTGCACGAGA ACGAAGTTCT TCGTCACCAG CTCTTTGCTG CTCCATACCG GGACCTGCCA 
GCCTCCCTCT CTGCCCCGAT GTCGGCAGCT CACCAGCTGC AGGCCATGCA CGCACAGTCA 
GCTGAGTTGC AGCGCTTGGC GCTGGAGCAG CAGCAGTGGC TGCACGCCCA TCACCCGCTG 
CACAGTGTGC CGCTGCCCGC CCAGGAGGAC TACTACAGTC ATCTGAAGAA GGAAAGCGAC 
AAGCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( NM_001205748.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192677.1 Bos taurus atrophin 1 (ATN1), mRNA.

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CloneID	OBa19790
Clone ID Related Accession (Same CDS sequence)	NM_001205748.1 , XM_005207132.3
Accession Version	NM_001205748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	atrophin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02014453.1. On Apr 20, 2011 this sequence version replaced XM_590763.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403639, SAMN02403640 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAA 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192677.1
CDS	351..3905
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	NM_001205748.1
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205748.1

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAA 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_005207132.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207189.1 Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.

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CloneID	OBa19790
Clone ID Related Accession (Same CDS sequence)	NM_001205748.1 , XM_005207132.3
Accession Version	XM_005207132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103940.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAA 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207189.1
CDS	301..3855
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_005207132.3
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207132.3

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGC AGGAGCTGCC CCGGCCCCAG 
TCTCCCTCCG ATCTGGACAG TCTGGATGGG CGGAGCCTCA ATGACGATGG CAGCAGTGAC 
CCCAGGGACA TCGACCAGGA CAACCGGAGC ACGTCTCCTA GCATCTACAG CCCTGGAAGC 
GTGGAGAATG ACTCCGACTC GTCTTCTGGC CTGTCCCAGG GCCCAGCCCG CCCCTATCAC 
CCACCTCCAC TCTTCCCTCC CTCCCCTCCA CCACCTGACA GCACCCCCCG ACAGCCAGAG 
CCTGGCTTTG AACCCCACCC TTCTGTGACA CCCACCGGAT ATCATGCTCC CATGGAGCCC 
CCCACATCTC GGATGTTCCA GGCTCCTCCA GGGGCTCCTC CCCCTCATCC ACAGCTCTAC 
CCTGGGGGCT CTGGTGGGGG AGTTTTGTCT GGACCCCCAC TGGGTCCCAA GGGGAGTGGG 
GCTGCCTCTT CAGTGGGGGC CCCTAGTGGG GGTAAGCAGC ACCCCCCACC CACCACCCCC 
ATTTCAATAT CAAGCTCTGG GGCTGGTGGT GCTCCCCCAA CGAAGCCACC TAACACTCCA 
GTCGGTGGTG GAAGCCTACC GTCTGCGCCA CCACCAGCCT CCTTCCCCCA CGTGACACCA 
AACCTGCCTC CCCCACCTGC TCTGCGACCC CTTAACAATG CATCGGCCTC TCCTCCTGGC 
CTGGGGGCCC AGCCACTACC TGGGCATCTC CCTTCCCCCC ATGCCATGGG GCAGAGCATG 
GGTGGACTTC CTCCTGGCCC GGAGAAGGGG CCCACTCTGG CTCCTTCACC CCATCCTCTG 
CCCCCTGCGT CCTCTTCAGC CCCTGCCCCC ACAATGAGGT ATCCTTACTC ATCCTCTACT 
AGCAGCTCTG CAGCAGCCGC CTCTTCCAAT TCTTCTGCCT CTGCCTCTGC CTCCCAGTAC 
CCCGCTTCCC AGGCACTGCC CAGTTATCCC CACTCCTTCC CTCCCCCGAC CAGTCTCTCT 
GTCTCTAATC AGCCCCCCAA GTATACGCAG CCTTCTCTTC CATCCCAGGC TGTGTGGAGC 
CAGGGTCCCC CTCCACCTCC TCCCTATGGC CGCCTCTTAG CCAACAGCAG TGCCCACCCA 
GGTCCCTTCC CTCCTTCAGC TGGAGGCCAG TCCACAGCCC ACCCATCAGC CCCAACACAT 
CACCACCACC ACCAGCAACA GCAGCAGCAG CAGCAGCAAC CACCACCACC ACATCATGGG 
GGCTCTGGGC CCCCTCCCCC TGGAGCATAT CCTCACCCCC TGGAGAGCGG TGGTTCCCAC 
CATGCACACC CCTATGCCAT GTCTCCCTCC CTGGGGTCTC TGAGACCCTA TCCACCAGGG 
CCAGCACACC TGCCCCCACC TCACAGCCAG GTGTCCTACA GCCAAGCAGG TCCTAATGGA 
CCCCCAACCT CCTCTTCTTC CAATTCCTCT TCCTCCTCTC AAGGGTCCTA CCCGGGTTCA 
CACCCCTCTC CTTCCCAGGG CCCCTCAGGG GCGCCCTACC CCTTTCCACC GGTGCCTCCG 
GTCACCACTT CCTCGGCCAC ACTTTCCACG GTCATTGCCA CAGTGGCTTC CTCGCCAGCA 
GGCTACAAGA CAGCCTCCCC ACCTGGGCCC CCGCCGTATG GCAAGCGAGC CCCGTCCCCA 
GGGGCCTACA AGACAGCCAC TCCACCTGGA TACAAGCCGG GGTCGCCGCC CTCCTTCCGA 
ACGGGGACCC CACCGGGCTA CCGAGGCGCC TCGCCGCCCG CAGGCCCAGG GACCTTCAAG 
CCGGGGTCGC CCACCGTGGG GCCAGGGCCG CTGCCGCCTG CGGGGCCCTC CGGCCTGTCG 
TCCCTGCCCG CACCGCCTGC GGCCCCTGCC TCTGGGCCGC CCCTGAGTGC CACGCAGATC 
AAACAGGAGC CGGCGGAGGA ATATGAGACC CCCGAGAGCC CGGTGCCCCC GGCCCGCAGC 
CCCTCGCCCC CTCCCAAGGT GGTAGATGTG CCCAGCCACG CCAGCCAGTC GGCCAGGTTC 
AACAAACACC TGGACCGCGG CTTTAACTCG TGCGCGCGCA GCGATCTGTA CTTCGTACCG 
CTGGAGGGGT CCAAGCTGGC CAAGAAACGG GCCGACCTGG TAGAGAAGGT GCGGCGCGAG 
GCCGAACAGC GCGCGCGCGA AGAAAAGGAG CGCGAGCGCG AGCGGGAGCG CGAGAAGGAA 
CGCGAGCGCG AGAAGGAGCG CGAGCTGGAA CGCAGCGTGA AGTTGGCTCA GGAGGGCCGT 
GCTCCAGTGG AGTGCCCATC TCTCGGCCCA GTGCCCCATC GCCCTCCGTT TGAGCCGGGC 
AGTGCTGTGG CTACAGTGCC CCCTTACCTG GGCCCCGACA CTCCAGCCCT ACGCACCCTC 
AGTGAATACG CCCGGCCCCA CGTGATGTCT CCTGGCAATC GCAACCATCC GTTCTACGTG 
CCCCTGGGGG CAGTGGACCC GGGGCTCCTG GGTTACAATG TCCCGGCCTT GTACAGCAGT 
GACCCAGCAG CCCGGGAGAG GGAGCGGGAA GCCCGTGAAC GAGACCTGCG TGACCGCCTC 
AAGCCTGGCT TTGAGGTGAA GCCCAGCGAG CTGGAACCCC TACATGGGGT CCCTGGGCCA 
GGCCTGGATC CCTTCCCCCG ACACGGGGGC CTGGCTTTGC AGCCTGGCCC CCCGGGCTTG 
CACCCTTTCC CCTTCCATCC GAGCCTGGGG CCGCTGGAGA GAGAACGTCT AGCTCTGGCA 
GCTGGGCCCG CCCTGCGGCC TGACATGTCC TACGCGGAGC GTCTGGCAGC TGAGAGGCAG 
CATGCAGAAA GGGTGGCCGC CCTGGGCAAT GACCCACTGG CGCGGCTGCA GATGCTCAAC 
GTGACCCCCC ATCACCACCA GCACTCCCAC ATCCATTCTC ACCTGCACCT CCACCAGCAG 
GATGCCATCC ACGCAGCCTC TGCCTCGGTG CACCCTCTCA TTGATCCCCT GGCCTCAGGG 
TCTCACCTTA CCCGGATCCC CTACCCAGCT GGGACCCTCC CCAACCCCCT TCTTCCTCAC 
CCTCTGCACG AGAACGAAGT TCTTCGTCAC CAGCTCTTTG CTGCTCCATA CCGGGACCTG 
CCAGCCTCCC TCTCTGCCCC GATGTCGGCA GCTCACCAGC TGCAGGCCAT GCACGCACAG 
TCAGCTGAGT TGCAGCGCTT GGCGCTGGAG CAGCAGCAGT GGCTGCACGC CCATCACCCG 
CTGCACAGTG TGCCGCTGCC CGCCCAGGAG GACTACTACA GTCATCTGAA GAAGGAAAGC 
GACAAGCCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATN1 ( XM_005207134.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207191.1 Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.

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CloneID	OBa19791
Clone ID Related Accession (Same CDS sequence)	XM_005207134.3
Accession Version	XM_005207134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	atrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103940.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGG AGCTGCCCCG GCCCCAGTCT 
CCCTCCGATC TGGACAGTCT GGATGGGCGG AGCCTCAATG ACGATGGCAG CAGTGACCCC 
AGGGACATCG ACCAGGACAA CCGGAGCACG TCTCCTAGCA TCTACAGCCC TGGAAGCGTG 
GAGAATGACT CCGACTCGTC TTCTGGCCTG TCCCAGGGCC CAGCCCGCCC CTATCACCCA 
CCTCCACTCT TCCCTCCCTC CCCTCCACCA CCTGACAGCA CCCCCCGACA GCCAGAGCCT 
GGCTTTGAAC CCCACCCTTC TGTGACACCC ACCGGATATC ATGCTCCCAT GGAGCCCCCC 
ACATCTCGGA TGTTCCAGGC TCCTCCAGGG GCTCCTCCCC CTCATCCACA GCTCTACCCT 
GGGGGCTCTG GTGGGGGAGT TTTGTCTGGA CCCCCACTGG GTCCCAAGGG GAGTGGGGCT 
GCCTCTTCAG TGGGGGCCCC TAGTGGGGGT AAGCAGCACC CCCCACCCAC CACCCCCATT 
TCAATATCAA GCTCTGGGGC TGGTGGTGCT CCCCCAACGA AGCCACCTAA CACTCCAGTC 
GGTGGTGGAA GCCTACCGTC TGCGCCACCA CCAGCCTCCT TCCCCCACGT GACACCAAAC 
CTGCCTCCCC CACCTGCTCT GCGACCCCTT AACAATGCAT CGGCCTCTCC TCCTGGCCTG 
GGGGCCCAGC CACTACCTGG GCATCTCCCT TCCCCCCATG CCATGGGGCA GAGCATGGGT 
GGACTTCCTC CTGGCCCGGA GAAGGGGCCC ACTCTGGCTC CTTCACCCCA TCCTCTGCCC 
CCTGCGTCCT CTTCAGCCCC TGCCCCCACA ATGAGGTATC CTTACTCATC CTCTACTAGC 
AGCTCTGCAG CAGCCGCCTC TTCCAATTCT TCTGCCTCTG CCTCTGCCTC CCAGTACCCC 
GCTTCCCAGG CACTGCCCAG TTATCCCCAC TCCTTCCCTC CCCCGACCAG TCTCTCTGTC 
TCTAATCAGC CCCCCAAGTA TACGCAGCCT TCTCTTCCAT CCCAGGCTGT GTGGAGCCAG 
GGTCCCCCTC CACCTCCTCC CTATGGCCGC CTCTTAGCCA ACAGCAGTGC CCACCCAGGT 
CCCTTCCCTC CTTCAGCTGG AGGCCAGTCC ACAGCCCACC CATCAGCCCC AACACATCAC 
CACCACCACC AGCAACAGCA GCAGCAGCAG CAGCAACCAC CACCACCACA TCATGGGGGC 
TCTGGGCCCC CTCCCCCTGG AGCATATCCT CACCCCCTGG AGAGCGGTGG TTCCCACCAT 
GCACACCCCT ATGCCATGTC TCCCTCCCTG GGGTCTCTGA GACCCTATCC ACCAGGGCCA 
GCACACCTGC CCCCACCTCA CAGCCAGGTG TCCTACAGCC AAGCAGGTCC TAATGGACCC 
CCAACCTCCT CTTCTTCCAA TTCCTCTTCC TCCTCTCAAG GGTCCTACCC GGGTTCACAC 
CCCTCTCCTT CCCAGGGCCC CTCAGGGGCG CCCTACCCCT TTCCACCGGT GCCTCCGGTC 
ACCACTTCCT CGGCCACACT TTCCACGGTC ATTGCCACAG TGGCTTCCTC GCCAGCAGGC 
TACAAGACAG CCTCCCCACC TGGGCCCCCG CCGTATGGCA AGCGAGCCCC GTCCCCAGGG 
GCCTACAAGA CAGCCACTCC ACCTGGATAC AAGCCGGGGT CGCCGCCCTC CTTCCGAACG 
GGGACCCCAC CGGGCTACCG AGGCGCCTCG CCGCCCGCAG GCCCAGGGAC CTTCAAGCCG 
GGGTCGCCCA CCGTGGGGCC AGGGCCGCTG CCGCCTGCGG GGCCCTCCGG CCTGTCGTCC 
CTGCCCGCAC CGCCTGCGGC CCCTGCCTCT GGGCCGCCCC TGAGTGCCAC GCAGATCAAA 
CAGGAGCCGG CGGAGGAATA TGAGACCCCC GAGAGCCCGG TGCCCCCGGC CCGCAGCCCC 
TCGCCCCCTC CCAAGGTGGT AGATGTGCCC AGCCACGCCA GCCAGTCGGC CAGGTTCAAC 
AAACACCTGG ACCGCGGCTT TAACTCGTGC GCGCGCAGCG ATCTGTACTT CGTACCGCTG 
GAGGGGTCCA AGCTGGCCAA GAAACGGGCC GACCTGGTAG AGAAGGTGCG GCGCGAGGCC 
GAACAGCGCG CGCGCGAAGA AAAGGAGCGC GAGCGCGAGC GGGAGCGCGA GAAGGAACGC 
GAGCGCGAGA AGGAGCGCGA GCTGGAACGC AGCGTGAAGT TGGCTCAGGA GGGCCGTGCT 
CCAGTGGAGT GCCCATCTCT CGGCCCAGTG CCCCATCGCC CTCCGTTTGA GCCGGGCAGT 
GCTGTGGCTA CAGTGCCCCC TTACCTGGGC CCCGACACTC CAGCCCTACG CACCCTCAGT 
GAATACGCCC GGCCCCACGT GATGTCTCCT GGCAATCGCA ACCATCCGTT CTACGTGCCC 
CTGGGGGCAG TGGACCCGGG GCTCCTGGGT TACAATGTCC CGGCCTTGTA CAGCAGTGAC 
CCAGCAGCCC GGGAGAGGGA GCGGGAAGCC CGTGAACGAG ACCTGCGTGA CCGCCTCAAG 
CCTGGCTTTG AGGTGAAGCC CAGCGAGCTG GAACCCCTAC ATGGGGTCCC TGGGCCAGGC 
CTGGATCCCT TCCCCCGACA CGGGGGCCTG GCTTTGCAGC CTGGCCCCCC GGGCTTGCAC 
CCTTTCCCCT TCCATCCGAG CCTGGGGCCG CTGGAGAGAG AACGTCTAGC TCTGGCAGCT 
GGGCCCGCCC TGCGGCCTGA CATGTCCTAC GCGGAGCGTC TGGCAGCTGA GAGGCAGCAT 
GCAGAAAGGG TGGCCGCCCT GGGCAATGAC CCACTGGCGC GGCTGCAGAT GCTCAACGTG 
ACCCCCCATC ACCACCAGCA CTCCCACATC CATTCTCACC TGCACCTCCA CCAGCAGGAT 
GCCATCCACG CAGCCTCTGC CTCGGTGCAC CCTCTCATTG ATCCCCTGGC CTCAGGGTCT 
CACCTTACCC GGATCCCCTA CCCAGCTGGG ACCCTCCCCA ACCCCCTTCT TCCTCACCCT 
CTGCACGAGA ACGAAGTTCT TCGTCACCAG CTCTTTGCTG CTCCATACCG GGACCTGCCA 
GCCTCCCTCT CTGCCCCGAT GTCGGCAGCT CACCAGCTGC AGGCCATGCA CGCACAGTCA 
GCTGAGTTGC AGCGCTTGGC GCTGGAGCAG CAGCAGTGGC TGCACGCCCA TCACCCGCTG 
CACAGTGTGC CGCTGCCCGC CCAGGAGGAC TACTACAGTC ATCTGAAGAA GGAAAGCGAC 
AAGCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207191.1
CDS	301..3852
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRPRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVSKQGRSEEISESESEETNAPKKTKTEELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPEPGFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGSGGGVLSGPPLGPKGSGAASSVGAPSGGKQHPPPTTPISISSSGAGGAPPTKPPNTPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQSMGGLPPGPEKGPTLAPSPHPLPPASSSAPAPTMRYPYSSSTSSSAAAASSNSSASASASQYPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSSAHPGPFPPSAGGQSTAHPSAPTHHHHHQQQQQQQQQPPPPHHGGSGPPPPGAYPHPLESGGSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPTSSSSNSSSSSQGSYPGSHPSPSQGPSGAPYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGASPPAGPGTFKPGSPTVGPGPLPPAGPSGLSSLPAPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	XM_005207134.3
Organism	Bos taurus(cattle)
Definition	Bos taurus atrophin 1 (ATN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207134.3

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCAATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCTGGGCCCC GGGAAGAGCT GAGACCGAGG GGCCGGGCCT CCCCTGGCGG GGTCAGCACG 
TCCAGCAGTG ATGGCAAAGC CGAGAAGTCT AGGCAGACGG CCAAGAAAGC CCGAGTAGAG 
GAAGCCTCCA CCCCAAAGGT CAGCAAGCAG GGCCGGAGTG AAGAGATCTC AGAGAGCGAA 
AGTGAGGAGA CCAATGCACC AAAAAAGACC AAAACTGAGG AGCTGCCCCG GCCCCAGTCT 
CCCTCCGATC TGGACAGTCT GGATGGGCGG AGCCTCAATG ACGATGGCAG CAGTGACCCC 
AGGGACATCG ACCAGGACAA CCGGAGCACG TCTCCTAGCA TCTACAGCCC TGGAAGCGTG 
GAGAATGACT CCGACTCGTC TTCTGGCCTG TCCCAGGGCC CAGCCCGCCC CTATCACCCA 
CCTCCACTCT TCCCTCCCTC CCCTCCACCA CCTGACAGCA CCCCCCGACA GCCAGAGCCT 
GGCTTTGAAC CCCACCCTTC TGTGACACCC ACCGGATATC ATGCTCCCAT GGAGCCCCCC 
ACATCTCGGA TGTTCCAGGC TCCTCCAGGG GCTCCTCCCC CTCATCCACA GCTCTACCCT 
GGGGGCTCTG GTGGGGGAGT TTTGTCTGGA CCCCCACTGG GTCCCAAGGG GAGTGGGGCT 
GCCTCTTCAG TGGGGGCCCC TAGTGGGGGT AAGCAGCACC CCCCACCCAC CACCCCCATT 
TCAATATCAA GCTCTGGGGC TGGTGGTGCT CCCCCAACGA AGCCACCTAA CACTCCAGTC 
GGTGGTGGAA GCCTACCGTC TGCGCCACCA CCAGCCTCCT TCCCCCACGT GACACCAAAC 
CTGCCTCCCC CACCTGCTCT GCGACCCCTT AACAATGCAT CGGCCTCTCC TCCTGGCCTG 
GGGGCCCAGC CACTACCTGG GCATCTCCCT TCCCCCCATG CCATGGGGCA GAGCATGGGT 
GGACTTCCTC CTGGCCCGGA GAAGGGGCCC ACTCTGGCTC CTTCACCCCA TCCTCTGCCC 
CCTGCGTCCT CTTCAGCCCC TGCCCCCACA ATGAGGTATC CTTACTCATC CTCTACTAGC 
AGCTCTGCAG CAGCCGCCTC TTCCAATTCT TCTGCCTCTG CCTCTGCCTC CCAGTACCCC 
GCTTCCCAGG CACTGCCCAG TTATCCCCAC TCCTTCCCTC CCCCGACCAG TCTCTCTGTC 
TCTAATCAGC CCCCCAAGTA TACGCAGCCT TCTCTTCCAT CCCAGGCTGT GTGGAGCCAG 
GGTCCCCCTC CACCTCCTCC CTATGGCCGC CTCTTAGCCA ACAGCAGTGC CCACCCAGGT 
CCCTTCCCTC CTTCAGCTGG AGGCCAGTCC ACAGCCCACC CATCAGCCCC AACACATCAC 
CACCACCACC AGCAACAGCA GCAGCAGCAG CAGCAACCAC CACCACCACA TCATGGGGGC 
TCTGGGCCCC CTCCCCCTGG AGCATATCCT CACCCCCTGG AGAGCGGTGG TTCCCACCAT 
GCACACCCCT ATGCCATGTC TCCCTCCCTG GGGTCTCTGA GACCCTATCC ACCAGGGCCA 
GCACACCTGC CCCCACCTCA CAGCCAGGTG TCCTACAGCC AAGCAGGTCC TAATGGACCC 
CCAACCTCCT CTTCTTCCAA TTCCTCTTCC TCCTCTCAAG GGTCCTACCC GGGTTCACAC 
CCCTCTCCTT CCCAGGGCCC CTCAGGGGCG CCCTACCCCT TTCCACCGGT GCCTCCGGTC 
ACCACTTCCT CGGCCACACT TTCCACGGTC ATTGCCACAG TGGCTTCCTC GCCAGCAGGC 
TACAAGACAG CCTCCCCACC TGGGCCCCCG CCGTATGGCA AGCGAGCCCC GTCCCCAGGG 
GCCTACAAGA CAGCCACTCC ACCTGGATAC AAGCCGGGGT CGCCGCCCTC CTTCCGAACG 
GGGACCCCAC CGGGCTACCG AGGCGCCTCG CCGCCCGCAG GCCCAGGGAC CTTCAAGCCG 
GGGTCGCCCA CCGTGGGGCC AGGGCCGCTG CCGCCTGCGG GGCCCTCCGG CCTGTCGTCC 
CTGCCCGCAC CGCCTGCGGC CCCTGCCTCT GGGCCGCCCC TGAGTGCCAC GCAGATCAAA 
CAGGAGCCGG CGGAGGAATA TGAGACCCCC GAGAGCCCGG TGCCCCCGGC CCGCAGCCCC 
TCGCCCCCTC CCAAGGTGGT AGATGTGCCC AGCCACGCCA GCCAGTCGGC CAGGTTCAAC 
AAACACCTGG ACCGCGGCTT TAACTCGTGC GCGCGCAGCG ATCTGTACTT CGTACCGCTG 
GAGGGGTCCA AGCTGGCCAA GAAACGGGCC GACCTGGTAG AGAAGGTGCG GCGCGAGGCC 
GAACAGCGCG CGCGCGAAGA AAAGGAGCGC GAGCGCGAGC GGGAGCGCGA GAAGGAACGC 
GAGCGCGAGA AGGAGCGCGA GCTGGAACGC AGCGTGAAGT TGGCTCAGGA GGGCCGTGCT 
CCAGTGGAGT GCCCATCTCT CGGCCCAGTG CCCCATCGCC CTCCGTTTGA GCCGGGCAGT 
GCTGTGGCTA CAGTGCCCCC TTACCTGGGC CCCGACACTC CAGCCCTACG CACCCTCAGT 
GAATACGCCC GGCCCCACGT GATGTCTCCT GGCAATCGCA ACCATCCGTT CTACGTGCCC 
CTGGGGGCAG TGGACCCGGG GCTCCTGGGT TACAATGTCC CGGCCTTGTA CAGCAGTGAC 
CCAGCAGCCC GGGAGAGGGA GCGGGAAGCC CGTGAACGAG ACCTGCGTGA CCGCCTCAAG 
CCTGGCTTTG AGGTGAAGCC CAGCGAGCTG GAACCCCTAC ATGGGGTCCC TGGGCCAGGC 
CTGGATCCCT TCCCCCGACA CGGGGGCCTG GCTTTGCAGC CTGGCCCCCC GGGCTTGCAC 
CCTTTCCCCT TCCATCCGAG CCTGGGGCCG CTGGAGAGAG AACGTCTAGC TCTGGCAGCT 
GGGCCCGCCC TGCGGCCTGA CATGTCCTAC GCGGAGCGTC TGGCAGCTGA GAGGCAGCAT 
GCAGAAAGGG TGGCCGCCCT GGGCAATGAC CCACTGGCGC GGCTGCAGAT GCTCAACGTG 
ACCCCCCATC ACCACCAGCA CTCCCACATC CATTCTCACC TGCACCTCCA CCAGCAGGAT 
GCCATCCACG CAGCCTCTGC CTCGGTGCAC CCTCTCATTG ATCCCCTGGC CTCAGGGTCT 
CACCTTACCC GGATCCCCTA CCCAGCTGGG ACCCTCCCCA ACCCCCTTCT TCCTCACCCT 
CTGCACGAGA ACGAAGTTCT TCGTCACCAG CTCTTTGCTG CTCCATACCG GGACCTGCCA 
GCCTCCCTCT CTGCCCCGAT GTCGGCAGCT CACCAGCTGC AGGCCATGCA CGCACAGTCA 
GCTGAGTTGC AGCGCTTGGC GCTGGAGCAG CAGCAGTGGC TGCACGCCCA TCACCCGCTG 
CACAGTGTGC CGCTGCCCGC CCAGGAGGAC TACTACAGTC ATCTGAAGAA GGAAAGCGAC 
AAGCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ATN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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