ARHGAP45 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP45
Entrez Gene ID	512972
Full Name	Rho GTPase activating protein 45
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205741.1	NP_001192670.1	minor histocompatibility protein HA-1
XM_024994155.1	XP_024849923.1	rho GTPase-activating protein 45 isoform X4
XM_024994153.1	XP_024849921.1	rho GTPase-activating protein 45 isoform X2
XM_024994156.1	XP_024849924.1	rho GTPase-activating protein 45 isoform X5
NM_001205741.2	NP_001192670.2	rho GTPase-activating protein 45
XM_024994152.1	XP_024849920.1	rho GTPase-activating protein 45 isoform X1
XM_024994154.1	XP_024849922.1	rho GTPase-activating protein 45 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	HMHA1	NP_036424.2
Gallus gallus (chicken)	HMHA1	NP_001026108.1
	HMHA1	XP_001117237.2
Mus musculus (house mouse)	Hmha1	NP_001136173.1
Xenopus tropicalis (tropical clawed frog)	hmha1	NP_001107971.1
Pan troglodytes (chimpanzee)	HMHA1	XP_003953357.1
Canis lupus familiaris (dog)	HMHA1	XP_005633213.1
Rattus norvegicus (Norway rat)	Hmha1	NP_001101537.1
Danio rerio (zebrafish)	LOC556955	XP_001919378.4
Danio rerio (zebrafish)	hmha1b	XP_683585.5
Bos taurus (cattle)	HMHA1	NP_001192670.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARHGAP45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235996	XM_024994155.1 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235997	XM_024994156.1 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa239026	NM_001205741.2 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235993	XM_024994152.1 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235994	XM_024994153.1 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235995	XM_024994154.1 Latest version!	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa39309	NM_001205741.1 Latest version!	Bos taurus histocompatibility (minor) HA-1 (HMHA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP45 ( XM_024994155.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849923.1 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa235996
Clone ID Related Accession (Same CDS sequence)	XM_024994155.1
Accession Version	XM_024994155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAGCATC CTCCTGTCCG TGCCCCCTGG GGACCCAGGC 
CAGTCCATGG AGAACCTGTA TGGACAGGCG AGTGAGAGTG CCCCCCCCAG CGGTGAGGAG 
TGTGATGCGG GCTGCCTGTC CCCGGAGGAC GTGGACACCC TGCTGCAGCG CTGTGAGGGG 
GGCGTGGACG CTGCCCTGCA GTACGCCAAG AACATGGCCA AATACATGAA GGACCTCATC 
GGCTACCTGG AGAAGCGGAG TGCGCTGGAG ATGGACTTTG CCAAAGGCCT GCAGAAGATC 
GTCCAGAACT GCAGACAGAG TGTCATGCAG GAGCCGCACA TGCCCCTCCT GTCCATCTAC 
TCACTGGCGC TGGAACAGGA CCTGGAGTTC GGCCATGGCC TGGTGCAGGC AGTGGGCACG 
CTGCTGACCC AGACCTTCAT GCAGCCCCTG AATCTGCGGC GGCTCGAACA TGAGAAGCGA 
AGGAAGGAGA TCAAAGAGTC CTGGCACCGC GCCCAGAGGA AGCTGCAAGA GGCAGAGTCC 
AATCTCCGCA AGGCCAAGCA GGGCTACATG CAGCGCTGCG AGGACCATGA CAAGGCCCGC 
TTCCTGGTGG CCAAGGCGGA GGAGGAGCAA GCCAACATTG GGCCCGGTGC AGGGGGTGCA 
GCCTCCAAGA CCCTGGACAA GCGTCGTCGC CTGGAGGAGG AGGCCAAGAA CAAGGCGGAG 
GAAGCAATGG CCACCTACCG TACATGCGTG GCCGATGCTA AGACACAGAA GCAGGAGCTG 
GAGGATACCA AGGTGACGGC ACTACGGCAG ATCCAGGAGG TCATCCGGCA GAGTGACCAG 
ACCATCAAGT CGGCCACCAT CTCATATTAC CAGATGATGC ACACGCAGAC GGCCCCACTG 
CCCGTGCACT TCCACATGCT GTATGAGAGC AGCAAGCTGT ACGACCCAGG CCAGCAGTAC 
GCTTCCCATG TGCGCCAGCT GCAGCGCGGC GAGGAGCCTG ATGTGCACTA CGACTTCGAG 
CCCCACGTGT CCGCCAGTGC CTGGTCCCCA GTCCTGCGTG CTCGGAAAAG CAGCTTCAAT 
GTCAGCGATG CTGTGGGGGC AGAGGCTGCC AGTAGCCCCC CGGAGGACGG CGGGCCCAGC 
CAGGGAGAGA TTGCCAAGGA GCGCAGGGGT GGGCGGGGCC ACCAGGTGCA CAAGTCGTGG 
CCCATCTCCA TCTCCGATTC GGAGGCCAGT CTGGACCCCA GCCCTGGCTC AGAGGACTTT 
AAGAAGTTTG AGCGTATGTC TTCCTGTGGC ACCATGTCGT CCAACGAGGA GCTGGCCGAC 
CAGGAGGGCA GTGCAGGGGC ATCAGCCTTT GATCAGGCTG ACCTCAATGG CATGACTCCA 
GAGCTGCCTG TGGCTGTGCC CAGCGGGCCC TTCCGCCACG TGGGGCTGTC CAAGGCGGCC 
CGGACCCACC GGCTTCGGAA GCTCCGCACA CCGGCCAAGT GCCGGGAATG CAACAGCTAC 
GTGTACTTCC AGGGCGCCGA GTGTGAGGAG TGCTGCCTGG CCTGCCACAA GAAGTGCCTG 
GAGACCCTGG CCATCCAGTG TGGCCACAAG AAGCTGCAGG GCCGTCTGCA GCTCTTTGGC 
CAGGACTTCA GCCGTGCTGC CTGCAGCACC CCCGACGGCG TGCCCTTCAT CATCAAGAAG 
TGCATCTTTG AGATCGAGCA GCGGGCGCTG CGCACCAAGG GCATCTACCG GGTCAATGGG 
GTGAAGACAC GTGTAGAGAA GCTGTGCCAG GCCTTCGAGA ATGGCAAGGA GCTGGTGGAA 
TTGTCGCAAG CCTCACCCCA CGACATCAGC AATGTCCTCA AGCTCTATCT GCGCCAGCTC 
CCTGAGCCGC TCATTTCCTT CCGCTTCTAC CATGAGCTTG TGGGGATGGC CAAAGACAGT 
CTGAAGGCCG AGGCCGAGGC CAAGGCAGCA TCTCGGGGTC GGCCGGATGC CACAGAGAGG 
GAGGCTGCAG CCATGGCCAT GGCGAGCCGG TTGCGGGAAC TCCTACGGGA CCTGCCTCGG 
GATAACTGGG CCACACTGCG GTACCTGATG CGGCACCTGC GCAGGATCGT GGAGGTGGAA 
CAGGACAACA AGATGACACC AGGGAACCTG GGCATTGTGT TTGGGCCCAC GCTGCTGCGG 
CCCCGGCCCA CTGAGGCCAC GGTGTCCCTT TCCTCCCTGG TGGACTACCC CCACCAGGCC 
TGCATCGTGG AGACCCTCAT CACCCACTTC AGCCTGGTCT TCAAGGAGGA GCCCGAAGAG 
GCCCCAGGGG GCCAGGATGG GGTATCCAGC CAGCGACCCG AGGTGGTGGT CCAGGCACCG 
TACCTGGGGG GCAGCGGGGG TGCAGCCTTT CCCTTGACAG AGGAGGCTGA GGATGGGGTC 
CTTGAACCCC ATGTCGCCTC CAATGACTCC GACTCAGAGC TGGAGGAGGC CTCAGACCTG 
CAGTCCCCGG TGGGTGGGGC TGCACTGCAC CGGCTCAGTT TTCTGGAGAG GCAGGGCGGC 
GAGGCAGGCA CAGAGGGCAG TCAGGGCAGC CGCAGCGGCA GCGAAGAGCA GCTGGGTGCC 
ACAGCCGGCG AGTACGGGCC TGGGGCCTGG GAGGGCCCTG GGGAGGAGCC AGCCCGGAGG 
CTCTCCGAGT ACAACACCAA CCAGTGCAAC AACACCAGCC AGTGCACCAC CACCACCCAG 
TGCAACGCCA CCAACCGGTG CAACAACGTG GCTGCAGCTT GGCTGCCAGC TGTGAGGCTG 
CGTGGTGGGC GGCTGGTAGG GGGCAACAGC TGCAAGAGGC AGCCAGAGTT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849923.1
CDS	192..3188
Translation	MHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSSILLSVPPGDPGQSMENLYGQASESAPPSGEECDAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQCTTTTQCNATNRCNNVAAAWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	XM_024994155.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994155.1

ORF Insert Sequence:

ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAGCATC CTCCTGTCCG TGCCCCCTGG GGACCCAGGC 
CAGTCCATGG AGAACCTGTA TGGACAGGCG AGTGAGAGTG CCCCCCCCAG CGGTGAGGAG 
TGTGATGCGG GCTGCCTGTC CCCGGAGGAC GTGGACACCC TGCTGCAGCG CTGTGAGGGG 
GGCGTGGACG CTGCCCTGCA GTACGCCAAG AACATGGCCA AATACATGAA GGACCTCATC 
GGCTACCTGG AGAAGCGGAG TGCGCTGGAG ATGGACTTTG CCAAAGGCCT GCAGAAGATC 
GTCCAGAACT GCAGACAGAG TGTCATGCAG GAGCCGCACA TGCCCCTCCT GTCCATCTAC 
TCACTGGCGC TGGAACAGGA CCTGGAGTTC GGCCATGGCC TGGTGCAGGC AGTGGGCACG 
CTGCTGACCC AGACCTTCAT GCAGCCCCTG AATCTGCGGC GGCTCGAACA TGAGAAGCGA 
AGGAAGGAGA TCAAAGAGTC CTGGCACCGC GCCCAGAGGA AGCTGCAAGA GGCAGAGTCC 
AATCTCCGCA AGGCCAAGCA GGGCTACATG CAGCGCTGCG AGGACCATGA CAAGGCCCGC 
TTCCTGGTGG CCAAGGCGGA GGAGGAGCAA GCCAACATTG GGCCCGGTGC AGGGGGTGCA 
GCCTCCAAGA CCCTGGACAA GCGTCGTCGC CTGGAGGAGG AGGCCAAGAA CAAGGCGGAG 
GAAGCAATGG CCACCTACCG TACATGCGTG GCCGATGCTA AGACACAGAA GCAGGAGCTG 
GAGGATACCA AGGTGACGGC ACTACGGCAG ATCCAGGAGG TCATCCGGCA GAGTGACCAG 
ACCATCAAGT CGGCCACCAT CTCATATTAC CAGATGATGC ACACGCAGAC GGCCCCACTG 
CCCGTGCACT TCCACATGCT GTATGAGAGC AGCAAGCTGT ACGACCCAGG CCAGCAGTAC 
GCTTCCCATG TGCGCCAGCT GCAGCGCGGC GAGGAGCCTG ATGTGCACTA CGACTTCGAG 
CCCCACGTGT CCGCCAGTGC CTGGTCCCCA GTCCTGCGTG CTCGGAAAAG CAGCTTCAAT 
GTCAGCGATG CTGTGGGGGC AGAGGCTGCC AGTAGCCCCC CGGAGGACGG CGGGCCCAGC 
CAGGGAGAGA TTGCCAAGGA GCGCAGGGGT GGGCGGGGCC ACCAGGTGCA CAAGTCGTGG 
CCCATCTCCA TCTCCGATTC GGAGGCCAGT CTGGACCCCA GCCCTGGCTC AGAGGACTTT 
AAGAAGTTTG AGCGTATGTC TTCCTGTGGC ACCATGTCGT CCAACGAGGA GCTGGCCGAC 
CAGGAGGGCA GTGCAGGGGC ATCAGCCTTT GATCAGGCTG ACCTCAATGG CATGACTCCA 
GAGCTGCCTG TGGCTGTGCC CAGCGGGCCC TTCCGCCACG TGGGGCTGTC CAAGGCGGCC 
CGGACCCACC GGCTTCGGAA GCTCCGCACA CCGGCCAAGT GCCGGGAATG CAACAGCTAC 
GTGTACTTCC AGGGCGCCGA GTGTGAGGAG TGCTGCCTGG CCTGCCACAA GAAGTGCCTG 
GAGACCCTGG CCATCCAGTG TGGCCACAAG AAGCTGCAGG GCCGTCTGCA GCTCTTTGGC 
CAGGACTTCA GCCGTGCTGC CTGCAGCACC CCCGACGGCG TGCCCTTCAT CATCAAGAAG 
TGCATCTTTG AGATCGAGCA GCGGGCGCTG CGCACCAAGG GCATCTACCG GGTCAATGGG 
GTGAAGACAC GTGTAGAGAA GCTGTGCCAG GCCTTCGAGA ATGGCAAGGA GCTGGTGGAA 
TTGTCGCAAG CCTCACCCCA CGACATCAGC AATGTCCTCA AGCTCTATCT GCGCCAGCTC 
CCTGAGCCGC TCATTTCCTT CCGCTTCTAC CATGAGCTTG TGGGGATGGC CAAAGACAGT 
CTGAAGGCCG AGGCCGAGGC CAAGGCAGCA TCTCGGGGTC GGCCGGATGC CACAGAGAGG 
GAGGCTGCAG CCATGGCCAT GGCGAGCCGG TTGCGGGAAC TCCTACGGGA CCTGCCTCGG 
GATAACTGGG CCACACTGCG GTACCTGATG CGGCACCTGC GCAGGATCGT GGAGGTGGAA 
CAGGACAACA AGATGACACC AGGGAACCTG GGCATTGTGT TTGGGCCCAC GCTGCTGCGG 
CCCCGGCCCA CTGAGGCCAC GGTGTCCCTT TCCTCCCTGG TGGACTACCC CCACCAGGCC 
TGCATCGTGG AGACCCTCAT CACCCACTTC AGCCTGGTCT TCAAGGAGGA GCCCGAAGAG 
GCCCCAGGGG GCCAGGATGG GGTATCCAGC CAGCGACCCG AGGTGGTGGT CCAGGCACCG 
TACCTGGGGG GCAGCGGGGG TGCAGCCTTT CCCTTGACAG AGGAGGCTGA GGATGGGGTC 
CTTGAACCCC ATGTCGCCTC CAATGACTCC GACTCAGAGC TGGAGGAGGC CTCAGACCTG 
CAGTCCCCGG TGGGTGGGGC TGCACTGCAC CGGCTCAGTT TTCTGGAGAG GCAGGGCGGC 
GAGGCAGGCA CAGAGGGCAG TCAGGGCAGC CGCAGCGGCA GCGAAGAGCA GCTGGGTGCC 
ACAGCCGGCG AGTACGGGCC TGGGGCCTGG GAGGGCCCTG GGGAGGAGCC AGCCCGGAGG 
CTCTCCGAGT ACAACACCAA CCAGTGCAAC AACACCAGCC AGTGCACCAC CACCACCCAG 
TGCAACGCCA CCAACCGGTG CAACAACGTG GCTGCAGCTT GGCTGCCAGC TGTGAGGCTG 
CGTGGTGGGC GGCTGGTAGG GGGCAACAGC TGCAAGAGGC AGCCAGAGTT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( XM_024994156.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849924.1 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa235997
Clone ID Related Accession (Same CDS sequence)	XM_024994156.1
Accession Version	XM_024994156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAG TGGACAGCAG CATCCTCCTG TCCGTGCCCC CTGGGGACCC AGGCCAGTCC 
ATGGAGAACC TGTATGGACA GGCGAGTGAG AGTGCCCCCC CCAGCGGTGA GGAGTGTGAT 
GCGGGCTGCC TGTCCCCGGA GGACGTGGAC ACCCTGCTGC AGCGCTGTGA GGGGGGCGTG 
GACGCTGCCC TGCAGTACGC CAAGAACATG GCCAAATACA TGAAGGACCT CATCGGCTAC 
CTGGAGAAGC GGAGTGCGCT GGAGATGGAC TTTGCCAAAG GCCTGCAGAA GATCGTCCAG 
AACTGCAGAC AGAGTGTCAT GCAGGAGCCG CACATGCCCC TCCTGTCCAT CTACTCACTG 
GCGCTGGAAC AGGACCTGGA GTTCGGCCAT GGCCTGGTGC AGGCAGTGGG CACGCTGCTG 
ACCCAGACCT TCATGCAGCC CCTGAATCTG CGGCGGCTCG AACATGAGAA GCGAAGGAAG 
GAGATCAAAG AGTCCTGGCA CCGCGCCCAG AGGAAGCTGC AAGAGGCAGA GTCCAATCTC 
CGCAAGGCCA AGCAGGGCTA CATGCAGCGC TGCGAGGACC ATGACAAGGC CCGCTTCCTG 
GTGGCCAAGG CGGAGGAGGA GCAAGCCAAC ATTGGGCCCG GTGCAGGGGG TGCAGCCTCC 
AAGACCCTGG ACAAGCGTCG TCGCCTGGAG GAGGAGGCCA AGAACAAGGC GGAGGAAGCA 
ATGGCCACCT ACCGTACATG CGTGGCCGAT GCTAAGACAC AGAAGCAGGA GCTGGAGGAT 
ACCAAGGTGA CGGCACTACG GCAGATCCAG GAGGTCATCC GGCAGAGTGA CCAGACCATC 
AAGTCGGCCA CCATCTCATA TTACCAGATG ATGCACACGC AGACGGCCCC ACTGCCCGTG 
CACTTCCACA TGCTGTATGA GAGCAGCAAG CTGTACGACC CAGGCCAGCA GTACGCTTCC 
CATGTGCGCC AGCTGCAGCG CGGCGAGGAG CCTGATGTGC ACTACGACTT CGAGCCCCAC 
GTGTCCGCCA GTGCCTGGTC CCCAGTCCTG CGTGCTCGGA AAAGCAGCTT CAATGTCAGC 
GATGCTGTGG GGGCAGAGGC TGCCAGTAGC CCCCCGGAGG ACGGCGGGCC CAGCCAGGGA 
GAGATTGCCA AGGAGCGCAG GGGTGGGCGG GGCCACCAGG TGCACAAGTC GTGGCCCATC 
TCCATCTCCG ATTCGGAGGC CAGTCTGGAC CCCAGCCCTG GCTCAGAGGA CTTTAAGAAG 
TTTGAGCGTA TGTCTTCCTG TGGCACCATG TCGTCCAACG AGGAGCTGGC CGACCAGGAG 
GGCAGTGCAG GGGCATCAGC CTTTGATCAG GCTGACCTCA ATGGCATGAC TCCAGAGCTG 
CCTGTGGCTG TGCCCAGCGG GCCCTTCCGC CACGTGGGGC TGTCCAAGGC GGCCCGGACC 
CACCGGCTTC GGAAGCTCCG CACACCGGCC AAGTGCCGGG AATGCAACAG CTACGTGTAC 
TTCCAGGGCG CCGAGTGTGA GGAGTGCTGC CTGGCCTGCC ACAAGAAGTG CCTGGAGACC 
CTGGCCATCC AGTGTGGCCA CAAGAAGCTG CAGGGCCGTC TGCAGCTCTT TGGCCAGGAC 
TTCAGCCGTG CTGCCTGCAG CACCCCCGAC GGCGTGCCCT TCATCATCAA GAAGTGCATC 
TTTGAGATCG AGCAGCGGGC GCTGCGCACC AAGGGCATCT ACCGGGTCAA TGGGGTGAAG 
ACACGTGTAG AGAAGCTGTG CCAGGCCTTC GAGAATGGCA AGGAGCTGGT GGAATTGTCG 
CAAGCCTCAC CCCACGACAT CAGCAATGTC CTCAAGCTCT ATCTGCGCCA GCTCCCTGAG 
CCGCTCATTT CCTTCCGCTT CTACCATGAG CTTGTGGGGA TGGCCAAAGA CAGTCTGAAG 
GCCGAGGCCG AGGCCAAGGC AGCATCTCGG GGTCGGCCGG ATGCCACAGA GAGGGAGGCT 
GCAGCCATGG CCATGGCGAG CCGGTTGCGG GAACTCCTAC GGGACCTGCC TCGGGATAAC 
TGGGCCACAC TGCGGTACCT GATGCGGCAC CTGCGCAGGA TCGTGGAGGT GGAACAGGAC 
AACAAGATGA CACCAGGGAA CCTGGGCATT GTGTTTGGGC CCACGCTGCT GCGGCCCCGG 
CCCACTGAGG CCACGGTGTC CCTTTCCTCC CTGGTGGACT ACCCCCACCA GGCCTGCATC 
GTGGAGACCC TCATCACCCA CTTCAGCCTG GTCTTCAAGG AGGAGCCCGA AGAGGCCCCA 
GGGGGCCAGG ATGGGGTATC CAGCCAGCGA CCCGAGGTGG TGGTCCAGGC ACCGTACCTG 
GGGGGCAGCG GGGGTGCAGC CTTTCCCTTG ACAGAGGAGG CTGAGGATGG GGTCCTTGAA 
CCCCATGTCG CCTCCAATGA CTCCGACTCA GAGCTGGAGG AGGCCTCAGA CCTGCAGTCC 
CCGGTGGGTG GGGCTGCACT GCACCGGCTC AGTTTTCTGG AGAGGCAGGG CGGCGAGGCA 
GGCACAGAGG GCAGTCAGGG CAGCCGCAGC GGCAGCGAAG AGCAGCTGGG TGCCACAGCC 
GGCGAGTACG GGCCTGGGGC CTGGGAGGGC CCTGGGGAGG AGCCAGCCCG GAGGCTCTCC 
GAGTACAACA CCAACCAGTG CAACAACACC AGCCAGTGCA CCACCACCAC CCAGTGCAAC 
GCCACCAACC GGTGCAACAA CGTGGCTGCA GCTTGGCTGC CAGCTGTGAG GCTGCGTGGT 
GGGCGGCTGG TAGGGGGCAA CAGCTGCAAG AGGCAGCCAG AGTTTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849924.1
CDS	87..2897
Translation	MGEVDSSILLSVPPGDPGQSMENLYGQASESAPPSGEECDAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQCTTTTQCNATNRCNNVAAAWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	XM_024994156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994156.1

ORF Insert Sequence:

ATGGGTGAAG TGGACAGCAG CATCCTCCTG TCCGTGCCCC CTGGGGACCC AGGCCAGTCC 
ATGGAGAACC TGTATGGACA GGCGAGTGAG AGTGCCCCCC CCAGCGGTGA GGAGTGTGAT 
GCGGGCTGCC TGTCCCCGGA GGACGTGGAC ACCCTGCTGC AGCGCTGTGA GGGGGGCGTG 
GACGCTGCCC TGCAGTACGC CAAGAACATG GCCAAATACA TGAAGGACCT CATCGGCTAC 
CTGGAGAAGC GGAGTGCGCT GGAGATGGAC TTTGCCAAAG GCCTGCAGAA GATCGTCCAG 
AACTGCAGAC AGAGTGTCAT GCAGGAGCCG CACATGCCCC TCCTGTCCAT CTACTCACTG 
GCGCTGGAAC AGGACCTGGA GTTCGGCCAT GGCCTGGTGC AGGCAGTGGG CACGCTGCTG 
ACCCAGACCT TCATGCAGCC CCTGAATCTG CGGCGGCTCG AACATGAGAA GCGAAGGAAG 
GAGATCAAAG AGTCCTGGCA CCGCGCCCAG AGGAAGCTGC AAGAGGCAGA GTCCAATCTC 
CGCAAGGCCA AGCAGGGCTA CATGCAGCGC TGCGAGGACC ATGACAAGGC CCGCTTCCTG 
GTGGCCAAGG CGGAGGAGGA GCAAGCCAAC ATTGGGCCCG GTGCAGGGGG TGCAGCCTCC 
AAGACCCTGG ACAAGCGTCG TCGCCTGGAG GAGGAGGCCA AGAACAAGGC GGAGGAAGCA 
ATGGCCACCT ACCGTACATG CGTGGCCGAT GCTAAGACAC AGAAGCAGGA GCTGGAGGAT 
ACCAAGGTGA CGGCACTACG GCAGATCCAG GAGGTCATCC GGCAGAGTGA CCAGACCATC 
AAGTCGGCCA CCATCTCATA TTACCAGATG ATGCACACGC AGACGGCCCC ACTGCCCGTG 
CACTTCCACA TGCTGTATGA GAGCAGCAAG CTGTACGACC CAGGCCAGCA GTACGCTTCC 
CATGTGCGCC AGCTGCAGCG CGGCGAGGAG CCTGATGTGC ACTACGACTT CGAGCCCCAC 
GTGTCCGCCA GTGCCTGGTC CCCAGTCCTG CGTGCTCGGA AAAGCAGCTT CAATGTCAGC 
GATGCTGTGG GGGCAGAGGC TGCCAGTAGC CCCCCGGAGG ACGGCGGGCC CAGCCAGGGA 
GAGATTGCCA AGGAGCGCAG GGGTGGGCGG GGCCACCAGG TGCACAAGTC GTGGCCCATC 
TCCATCTCCG ATTCGGAGGC CAGTCTGGAC CCCAGCCCTG GCTCAGAGGA CTTTAAGAAG 
TTTGAGCGTA TGTCTTCCTG TGGCACCATG TCGTCCAACG AGGAGCTGGC CGACCAGGAG 
GGCAGTGCAG GGGCATCAGC CTTTGATCAG GCTGACCTCA ATGGCATGAC TCCAGAGCTG 
CCTGTGGCTG TGCCCAGCGG GCCCTTCCGC CACGTGGGGC TGTCCAAGGC GGCCCGGACC 
CACCGGCTTC GGAAGCTCCG CACACCGGCC AAGTGCCGGG AATGCAACAG CTACGTGTAC 
TTCCAGGGCG CCGAGTGTGA GGAGTGCTGC CTGGCCTGCC ACAAGAAGTG CCTGGAGACC 
CTGGCCATCC AGTGTGGCCA CAAGAAGCTG CAGGGCCGTC TGCAGCTCTT TGGCCAGGAC 
TTCAGCCGTG CTGCCTGCAG CACCCCCGAC GGCGTGCCCT TCATCATCAA GAAGTGCATC 
TTTGAGATCG AGCAGCGGGC GCTGCGCACC AAGGGCATCT ACCGGGTCAA TGGGGTGAAG 
ACACGTGTAG AGAAGCTGTG CCAGGCCTTC GAGAATGGCA AGGAGCTGGT GGAATTGTCG 
CAAGCCTCAC CCCACGACAT CAGCAATGTC CTCAAGCTCT ATCTGCGCCA GCTCCCTGAG 
CCGCTCATTT CCTTCCGCTT CTACCATGAG CTTGTGGGGA TGGCCAAAGA CAGTCTGAAG 
GCCGAGGCCG AGGCCAAGGC AGCATCTCGG GGTCGGCCGG ATGCCACAGA GAGGGAGGCT 
GCAGCCATGG CCATGGCGAG CCGGTTGCGG GAACTCCTAC GGGACCTGCC TCGGGATAAC 
TGGGCCACAC TGCGGTACCT GATGCGGCAC CTGCGCAGGA TCGTGGAGGT GGAACAGGAC 
AACAAGATGA CACCAGGGAA CCTGGGCATT GTGTTTGGGC CCACGCTGCT GCGGCCCCGG 
CCCACTGAGG CCACGGTGTC CCTTTCCTCC CTGGTGGACT ACCCCCACCA GGCCTGCATC 
GTGGAGACCC TCATCACCCA CTTCAGCCTG GTCTTCAAGG AGGAGCCCGA AGAGGCCCCA 
GGGGGCCAGG ATGGGGTATC CAGCCAGCGA CCCGAGGTGG TGGTCCAGGC ACCGTACCTG 
GGGGGCAGCG GGGGTGCAGC CTTTCCCTTG ACAGAGGAGG CTGAGGATGG GGTCCTTGAA 
CCCCATGTCG CCTCCAATGA CTCCGACTCA GAGCTGGAGG AGGCCTCAGA CCTGCAGTCC 
CCGGTGGGTG GGGCTGCACT GCACCGGCTC AGTTTTCTGG AGAGGCAGGG CGGCGAGGCA 
GGCACAGAGG GCAGTCAGGG CAGCCGCAGC GGCAGCGAAG AGCAGCTGGG TGCCACAGCC 
GGCGAGTACG GGCCTGGGGC CTGGGAGGGC CCTGGGGAGG AGCCAGCCCG GAGGCTCTCC 
GAGTACAACA CCAACCAGTG CAACAACACC AGCCAGTGCA CCACCACCAC CCAGTGCAAC 
GCCACCAACC GGTGCAACAA CGTGGCTGCA GCTTGGCTGC CAGCTGTGAG GCTGCGTGGT 
GGGCGGCTGG TAGGGGGCAA CAGCTGCAAG AGGCAGCCAG AGTTTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( NM_001205741.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192670.2 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), mRNA.

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CloneID	OBa239026
Clone ID Related Accession (Same CDS sequence)	NM_001205741.2
Accession Version	NM_001205741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-14
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On May 30, 2018 this sequence version replaced NM_001205741.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAACTGG GGTGGTGGAT GGGAGGAGGG GTGGGCTGGG 
GCAGGGGAGG GCTGGGCAGG AGTGGAGCCT GGGCTGAGGG GTGTCTACTC TGCAGGCTGC 
CTGTCCCCGG AGGACGTGGA CACCCTGCTG CAGCGCTGTG AGGGGGGCGT GGACGCTGCC 
CTGCAGTACG CCAAGAACAT GGCCAAATAC ATGAAGGACC TCATCGGCTA CCTGGAGAAG 
CGGAGTGCGC TGGAGATGGA CTTTGCCAAA GGCCTGCAGA AGATCGTCCA GAACTGCAGA 
CAGAGTGTCA TGCAGGAGCC GCACATGCCC CTCCTGTCCA TCTACTCACT GGCGCTGGAA 
CAGGACCTGG AGTTCGGCCA TGGCCTGGTG CAGGCAGTGG GCACGCTGCT GACCCAGACC 
TTCATGCAGC CCCTGAATCT GCGGCGGCTC GAACATGAGA AGCGAAGGAA GGAGATCAAA 
GAGTCCTGGC ACCGCGCCCA GAGGAAGCTG CAAGAGGCAG AGTCCAATCT CCGCAAGGCC 
AAGCAGGGCT ACATGCAGCG CTGCGAGGAC CATGACAAGG CCCGCTTCCT GGTGGCCAAG 
GCGGAGGAGG AGCAAGCCAA CATTGGGCCC GGTGCAGGGG GTGCAGCCTC CAAGACCCTG 
GACAAGCGTC GTCGCCTGGA GGAGGAGGCC AAGAACAAGG CGGAGGAAGC AATGGCCACC 
TACCGTACAT GCGTGGCCGA TGCTAAGACA CAGAAGCAGG AGCTGGAGGA TACCAAGGTG 
ACGGCACTAC GGCAGATCCA GGAGGTCATC CGGCAGAGTG ACCAGACCAT CAAGTCGGCC 
ACCATCTCAT ATTACCAGAT GATGCACACG CAGACGGCCC CACTGCCCGT GCACTTCCAC 
ATGCTGTATG AGAGCAGCAA GCTGTACGAC CCAGGCCAGC AGTACGCTTC CCATGTGCGC 
CAGCTGCAGC GCGGCGAGGA GCCTGATGTG CACTACGACT TCGAGCCCCA CGTGTCCGCC 
AGTGCCTGGT CCCCAGTCCT GCGTGCTCGG AAAAGCAGCT TCAATGTCAG CGATGCTGTG 
GGGGCAGAGG CTGCCAGTAG CCCCCCGGAG GACGGCGGGC CCAGCCAGGG AGAGATTGCC 
AAGGAGCGCA GGGGTGGGCG GGGCCACCAG GTGCACAAGT CGTGGCCCAT CTCCATCTCC 
GATTCGGAGG CCAGTCTGGA CCCCAGCCCT GGCTCAGAGG ACTTTAAGAA GTTTGAGCGT 
ATGTCTTCCT GTGGCACCAT GTCGTCCAAC GAGGAGCTGG CCGACCAGGA GGGCAGTGCA 
GGGGCATCAG CCTTTGATCA GGCTGACCTC AATGGCATGA CTCCAGAGCT GCCTGTGGCT 
GTGCCCAGCG GGCCCTTCCG CCACGTGGGG CTGTCCAAGG CGGCCCGGAC CCACCGGCTT 
CGGAAGCTCC GCACACCGGC CAAGTGCCGG GAATGCAACA GCTACGTGTA CTTCCAGGGC 
GCCGAGTGTG AGGAGTGCTG CCTGGCCTGC CACAAGAAGT GCCTGGAGAC CCTGGCCATC 
CAGTGTGGCC ACAAGAAGCT GCAGGGCCGT CTGCAGCTCT TTGGCCAGGA CTTCAGCCGT 
GCTGCCTGCA GCACCCCCGA CGGCGTGCCC TTCATCATCA AGAAGTGCAT CTTTGAGATC 
GAGCAGCGGG CGCTGCGCAC CAAGGGCATC TACCGGGTCA ATGGGGTGAA GACACGTGTA 
GAGAAGCTGT GCCAGGCCTT CGAGAATGGC AAGGAGCTGG TGGAATTGTC GCAAGCCTCA 
CCCCACGACA TCAGCAATGT CCTCAAGCTC TATCTGCGCC AGCTCCCTGA GCCGCTCATT 
TCCTTCCGCT TCTACCATGA GCTTGTGGGG ATGGCCAAAG ACAGTCTGAA GGCCGAGGCC 
GAGGCCAAGG CAGCATCTCG GGGTCGGCCG GATGCCACAG AGAGGGAGGC TGCAGCCATG 
GCCATGGCGA GCCGGTTGCG GGAACTCCTA CGGGACCTGC CTCGGGATAA CTGGGCCACA 
CTGCGGTACC TGATGCGGCA CCTGCGCAGG ATCGTGGAGG TGGAACAGGA CAACAAGATG 
ACACCAGGGA ACCTGGGCAT TGTGTTTGGG CCCACGCTGC TGCGGCCCCG GCCCACTGAG 
GCCACGGTGT CCCTTTCCTC CCTGGTGGAC TACCCCCACC AGGCCTGCAT CGTGGAGACC 
CTCATCACCC ACTTCAGCCT GGTCTTCAAG GAGGAGCCCG AAGAGGCCCC AGGGGGCCAG 
GATGGGGTAT CCAGCCAGCG ACCCGAGGTG GTGGTCCAGG CACCGTACCT GGGGGGCAGC 
GGGGGTGCAG CCTTTCCCTT GACAGAGGAG GCTGAGGATG GGGTCCTTGA ACCCCATGTC 
GCCTCCAATG ACTCCGACTC AGAGCTGGAG GAGGCCTCAG ACCTGCAGTC CCCGGTGGGT 
GGGGCTGCAC TGCACCGGCT CAGTTTTCTG GAGAGGCAGG GCGGCGAGGC AGGCACAGAG 
GGCAGTCAGG GCAGCCGCAG CGGCAGCGAA GAGCAGCTGG GTGCCACAGC CGGCGAGTAC 
GGGCCTGGGG CCTGGGAGGG CCCTGGGGAG GAGCCAGCCC GGAGGCTCTC CGAGTACAAC 
ACCAACCAGT GCAACAACAC CAGCCAGTCT TGGCTGCCAG CTGTGAGGCT GCGTGGTGGG 
CGGCTGGTAG GGGGCAACAG CTGCAAGAGG CAGCCAGAGT TTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192670.2
CDS	1..3408
Translation	MFSRKKRELMKTPSISKKNRAGSPCPQPSGELPRRDGADAVSLGPSLEPPSVASNAKATGTLKRPTSLSRHASAAGFPLSGASTWTLGRSHRSPLSAASPAEAPIQGPCPDTVEDISHLLADVARFAEGLEKLKECVLHDELLEARRPLAHECLGEALRVMHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSNWGGGWEEGWAGAGEGWAGVEPGLRGVYSAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQSWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	NM_001205741.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205741.2

ORF Insert Sequence:

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAACTGG GGTGGTGGAT GGGAGGAGGG GTGGGCTGGG 
GCAGGGGAGG GCTGGGCAGG AGTGGAGCCT GGGCTGAGGG GTGTCTACTC TGCAGGCTGC 
CTGTCCCCGG AGGACGTGGA CACCCTGCTG CAGCGCTGTG AGGGGGGCGT GGACGCTGCC 
CTGCAGTACG CCAAGAACAT GGCCAAATAC ATGAAGGACC TCATCGGCTA CCTGGAGAAG 
CGGAGTGCGC TGGAGATGGA CTTTGCCAAA GGCCTGCAGA AGATCGTCCA GAACTGCAGA 
CAGAGTGTCA TGCAGGAGCC GCACATGCCC CTCCTGTCCA TCTACTCACT GGCGCTGGAA 
CAGGACCTGG AGTTCGGCCA TGGCCTGGTG CAGGCAGTGG GCACGCTGCT GACCCAGACC 
TTCATGCAGC CCCTGAATCT GCGGCGGCTC GAACATGAGA AGCGAAGGAA GGAGATCAAA 
GAGTCCTGGC ACCGCGCCCA GAGGAAGCTG CAAGAGGCAG AGTCCAATCT CCGCAAGGCC 
AAGCAGGGCT ACATGCAGCG CTGCGAGGAC CATGACAAGG CCCGCTTCCT GGTGGCCAAG 
GCGGAGGAGG AGCAAGCCAA CATTGGGCCC GGTGCAGGGG GTGCAGCCTC CAAGACCCTG 
GACAAGCGTC GTCGCCTGGA GGAGGAGGCC AAGAACAAGG CGGAGGAAGC AATGGCCACC 
TACCGTACAT GCGTGGCCGA TGCTAAGACA CAGAAGCAGG AGCTGGAGGA TACCAAGGTG 
ACGGCACTAC GGCAGATCCA GGAGGTCATC CGGCAGAGTG ACCAGACCAT CAAGTCGGCC 
ACCATCTCAT ATTACCAGAT GATGCACACG CAGACGGCCC CACTGCCCGT GCACTTCCAC 
ATGCTGTATG AGAGCAGCAA GCTGTACGAC CCAGGCCAGC AGTACGCTTC CCATGTGCGC 
CAGCTGCAGC GCGGCGAGGA GCCTGATGTG CACTACGACT TCGAGCCCCA CGTGTCCGCC 
AGTGCCTGGT CCCCAGTCCT GCGTGCTCGG AAAAGCAGCT TCAATGTCAG CGATGCTGTG 
GGGGCAGAGG CTGCCAGTAG CCCCCCGGAG GACGGCGGGC CCAGCCAGGG AGAGATTGCC 
AAGGAGCGCA GGGGTGGGCG GGGCCACCAG GTGCACAAGT CGTGGCCCAT CTCCATCTCC 
GATTCGGAGG CCAGTCTGGA CCCCAGCCCT GGCTCAGAGG ACTTTAAGAA GTTTGAGCGT 
ATGTCTTCCT GTGGCACCAT GTCGTCCAAC GAGGAGCTGG CCGACCAGGA GGGCAGTGCA 
GGGGCATCAG CCTTTGATCA GGCTGACCTC AATGGCATGA CTCCAGAGCT GCCTGTGGCT 
GTGCCCAGCG GGCCCTTCCG CCACGTGGGG CTGTCCAAGG CGGCCCGGAC CCACCGGCTT 
CGGAAGCTCC GCACACCGGC CAAGTGCCGG GAATGCAACA GCTACGTGTA CTTCCAGGGC 
GCCGAGTGTG AGGAGTGCTG CCTGGCCTGC CACAAGAAGT GCCTGGAGAC CCTGGCCATC 
CAGTGTGGCC ACAAGAAGCT GCAGGGCCGT CTGCAGCTCT TTGGCCAGGA CTTCAGCCGT 
GCTGCCTGCA GCACCCCCGA CGGCGTGCCC TTCATCATCA AGAAGTGCAT CTTTGAGATC 
GAGCAGCGGG CGCTGCGCAC CAAGGGCATC TACCGGGTCA ATGGGGTGAA GACACGTGTA 
GAGAAGCTGT GCCAGGCCTT CGAGAATGGC AAGGAGCTGG TGGAATTGTC GCAAGCCTCA 
CCCCACGACA TCAGCAATGT CCTCAAGCTC TATCTGCGCC AGCTCCCTGA GCCGCTCATT 
TCCTTCCGCT TCTACCATGA GCTTGTGGGG ATGGCCAAAG ACAGTCTGAA GGCCGAGGCC 
GAGGCCAAGG CAGCATCTCG GGGTCGGCCG GATGCCACAG AGAGGGAGGC TGCAGCCATG 
GCCATGGCGA GCCGGTTGCG GGAACTCCTA CGGGACCTGC CTCGGGATAA CTGGGCCACA 
CTGCGGTACC TGATGCGGCA CCTGCGCAGG ATCGTGGAGG TGGAACAGGA CAACAAGATG 
ACACCAGGGA ACCTGGGCAT TGTGTTTGGG CCCACGCTGC TGCGGCCCCG GCCCACTGAG 
GCCACGGTGT CCCTTTCCTC CCTGGTGGAC TACCCCCACC AGGCCTGCAT CGTGGAGACC 
CTCATCACCC ACTTCAGCCT GGTCTTCAAG GAGGAGCCCG AAGAGGCCCC AGGGGGCCAG 
GATGGGGTAT CCAGCCAGCG ACCCGAGGTG GTGGTCCAGG CACCGTACCT GGGGGGCAGC 
GGGGGTGCAG CCTTTCCCTT GACAGAGGAG GCTGAGGATG GGGTCCTTGA ACCCCATGTC 
GCCTCCAATG ACTCCGACTC AGAGCTGGAG GAGGCCTCAG ACCTGCAGTC CCCGGTGGGT 
GGGGCTGCAC TGCACCGGCT CAGTTTTCTG GAGAGGCAGG GCGGCGAGGC AGGCACAGAG 
GGCAGTCAGG GCAGCCGCAG CGGCAGCGAA GAGCAGCTGG GTGCCACAGC CGGCGAGTAC 
GGGCCTGGGG CCTGGGAGGG CCCTGGGGAG GAGCCAGCCC GGAGGCTCTC CGAGTACAAC 
ACCAACCAGT GCAACAACAC CAGCCAGTCT TGGCTGCCAG CTGTGAGGCT GCGTGGTGGG 
CGGCTGGTAG GGGGCAACAG CTGCAAGAGG CAGCCAGAGT TTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( XM_024994152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849920.1 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X1, mRNA.

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CloneID	OBa235993
Clone ID Related Accession (Same CDS sequence)	XM_024994152.1
Accession Version	XM_024994152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCC AGTGGCGGGG GGTACGAGCC AGCTACAGCC CCCACCAGGC TGGACTGCAG 
GGGCTTCGCA GGATGGGCTG GGATCCTCGC ATTCAGCTTA CGGAGCTGCC TAGGAGAGAC 
GGGGCTGATG CAGTGTCCCT GGGGCCCAGC CTAGAACCCC CAAGTGTGGC CTCGAATGCT 
AAGGCCACAG GCACTCTCAA GAGGCCAACC AGCCTGAGCC GCCATGCCAG CGCTGCTGGC 
TTCCCTCTGT CGGGGGCCAG CACTTGGACG CTGGGCCGCA GCCATCGCAG CCCACTGTCT 
GCGGCCAGCC CGGCCGAGGC ACCCATCCAG GGACCCTGCC CAGACACCGT GGAGGACATC 
TCCCACCTGC TGGCTGACGT GGCCCGCTTT GCCGAGGGTC TTGAGAAACT GAAGGAGTGT 
GTTCTGCATG ATGAACTCCT TGAGGCCCGC CGGCCACTGG CCCATGAGTG CCTGGGTGAG 
GCCCTCCGTG TGATGCACCA GGTCATCTCC AAGTACCCAC TGCTGAACAC TGTGGAGACA 
CTCACGGCTG CTGGCACCCT CATTGCCAAA GTCAAAGCCT TCCATTATGA GTGCAACAAT 
GAGTCAGACA AGCAGGAGTT TGAGAAGGCC TTGGAGACCA TTGCTGTCTC CTTTAGCAGC 
ACTGTATCCG AGTTCCTCAT GGGTGAAGTG GACAGCAGCA TCCTCCTGTC CGTGCCCCCT 
GGGGACCCAG GCCAGTCCAT GGAGAACCTG TATGGACAGG CGAGTGAGAG TGCCCCCCCC 
AGCGGTGAGG AGTGTGATGC GGGCTGCCTG TCCCCGGAGG ACGTGGACAC CCTGCTGCAG 
CGCTGTGAGG GGGGCGTGGA CGCTGCCCTG CAGTACGCCA AGAACATGGC CAAATACATG 
AAGGACCTCA TCGGCTACCT GGAGAAGCGG AGTGCGCTGG AGATGGACTT TGCCAAAGGC 
CTGCAGAAGA TCGTCCAGAA CTGCAGACAG AGTGTCATGC AGGAGCCGCA CATGCCCCTC 
CTGTCCATCT ACTCACTGGC GCTGGAACAG GACCTGGAGT TCGGCCATGG CCTGGTGCAG 
GCAGTGGGCA CGCTGCTGAC CCAGACCTTC ATGCAGCCCC TGAATCTGCG GCGGCTCGAA 
CATGAGAAGC GAAGGAAGGA GATCAAAGAG TCCTGGCACC GCGCCCAGAG GAAGCTGCAA 
GAGGCAGAGT CCAATCTCCG CAAGGCCAAG CAGGGCTACA TGCAGCGCTG CGAGGACCAT 
GACAAGGCCC GCTTCCTGGT GGCCAAGGCG GAGGAGGAGC AAGCCAACAT TGGGCCCGGT 
GCAGGGGGTG CAGCCTCCAA GACCCTGGAC AAGCGTCGTC GCCTGGAGGA GGAGGCCAAG 
AACAAGGCGG AGGAAGCAAT GGCCACCTAC CGTACATGCG TGGCCGATGC TAAGACACAG 
AAGCAGGAGC TGGAGGATAC CAAGGTGACG GCACTACGGC AGATCCAGGA GGTCATCCGG 
CAGAGTGACC AGACCATCAA GTCGGCCACC ATCTCATATT ACCAGATGAT GCACACGCAG 
ACGGCCCCAC TGCCCGTGCA CTTCCACATG CTGTATGAGA GCAGCAAGCT GTACGACCCA 
GGCCAGCAGT ACGCTTCCCA TGTGCGCCAG CTGCAGCGCG GCGAGGAGCC TGATGTGCAC 
TACGACTTCG AGCCCCACGT GTCCGCCAGT GCCTGGTCCC CAGTCCTGCG TGCTCGGAAA 
AGCAGCTTCA ATGTCAGCGA TGCTGTGGGG GCAGAGGCTG CCAGTAGCCC CCCGGAGGAC 
GGCGGGCCCA GCCAGGGAGA GATTGCCAAG GAGCGCAGGG GTGGGCGGGG CCACCAGGTG 
CACAAGTCGT GGCCCATCTC CATCTCCGAT TCGGAGGCCA GTCTGGACCC CAGCCCTGGC 
TCAGAGGACT TTAAGAAGTT TGAGCGTATG TCTTCCTGTG GCACCATGTC GTCCAACGAG 
GAGCTGGCCG ACCAGGAGGG CAGTGCAGGG GCATCAGCCT TTGATCAGGC TGACCTCAAT 
GGCATGACTC CAGAGCTGCC TGTGGCTGTG CCCAGCGGGC CCTTCCGCCA CGTGGGGCTG 
TCCAAGGCGG CCCGGACCCA CCGGCTTCGG AAGCTCCGCA CACCGGCCAA GTGCCGGGAA 
TGCAACAGCT ACGTGTACTT CCAGGGCGCC GAGTGTGAGG AGTGCTGCCT GGCCTGCCAC 
AAGAAGTGCC TGGAGACCCT GGCCATCCAG TGTGGCCACA AGAAGCTGCA GGGCCGTCTG 
CAGCTCTTTG GCCAGGACTT CAGCCGTGCT GCCTGCAGCA CCCCCGACGG CGTGCCCTTC 
ATCATCAAGA AGTGCATCTT TGAGATCGAG CAGCGGGCGC TGCGCACCAA GGGCATCTAC 
CGGGTCAATG GGGTGAAGAC ACGTGTAGAG AAGCTGTGCC AGGCCTTCGA GAATGGCAAG 
GAGCTGGTGG AATTGTCGCA AGCCTCACCC CACGACATCA GCAATGTCCT CAAGCTCTAT 
CTGCGCCAGC TCCCTGAGCC GCTCATTTCC TTCCGCTTCT ACCATGAGCT TGTGGGGATG 
GCCAAAGACA GTCTGAAGGC CGAGGCCGAG GCCAAGGCAG CATCTCGGGG TCGGCCGGAT 
GCCACAGAGA GGGAGGCTGC AGCCATGGCC ATGGCGAGCC GGTTGCGGGA ACTCCTACGG 
GACCTGCCTC GGGATAACTG GGCCACACTG CGGTACCTGA TGCGGCACCT GCGCAGGATC 
GTGGAGGTGG AACAGGACAA CAAGATGACA CCAGGGAACC TGGGCATTGT GTTTGGGCCC 
ACGCTGCTGC GGCCCCGGCC CACTGAGGCC ACGGTGTCCC TTTCCTCCCT GGTGGACTAC 
CCCCACCAGG CCTGCATCGT GGAGACCCTC ATCACCCACT TCAGCCTGGT CTTCAAGGAG 
GAGCCCGAAG AGGCCCCAGG GGGCCAGGAT GGGGTATCCA GCCAGCGACC CGAGGTGGTG 
GTCCAGGCAC CGTACCTGGG GGGCAGCGGG GGTGCAGCCT TTCCCTTGAC AGAGGAGGCT 
GAGGATGGGG TCCTTGAACC CCATGTCGCC TCCAATGACT CCGACTCAGA GCTGGAGGAG 
GCCTCAGACC TGCAGTCCCC GGTGGGTGGG GCTGCACTGC ACCGGCTCAG TTTTCTGGAG 
AGGCAGGGCG GCGAGGCAGG CACAGAGGGC AGTCAGGGCA GCCGCAGCGG CAGCGAAGAG 
CAGCTGGGTG CCACAGCCGG CGAGTACGGG CCTGGGGCCT GGGAGGGCCC TGGGGAGGAG 
CCAGCCCGGA GGCTCTCCGA GTACAACACC AACCAGTGCA ACAACACCAG CCAGTGCACC 
ACCACCACCC AGTGCAACGC CACCAACCGG TGCAACAACG TGGCTGCAGC TTGGCTGCCA 
GCTGTGAGGC TGCGTGGTGG GCGGCTGGTA GGGGGCAACA GCTGCAAGAG GCAGCCAGAG 
TTTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849920.1
CDS	72..3560
Translation	MLGQWRGVRASYSPHQAGLQGLRRMGWDPRIQLTELPRRDGADAVSLGPSLEPPSVASNAKATGTLKRPTSLSRHASAAGFPLSGASTWTLGRSHRSPLSAASPAEAPIQGPCPDTVEDISHLLADVARFAEGLEKLKECVLHDELLEARRPLAHECLGEALRVMHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSSILLSVPPGDPGQSMENLYGQASESAPPSGEECDAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQCTTTTQCNATNRCNNVAAAWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	XM_024994152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994152.1

ORF Insert Sequence:

ATGCTGGGCC AGTGGCGGGG GGTACGAGCC AGCTACAGCC CCCACCAGGC TGGACTGCAG 
GGGCTTCGCA GGATGGGCTG GGATCCTCGC ATTCAGCTTA CGGAGCTGCC TAGGAGAGAC 
GGGGCTGATG CAGTGTCCCT GGGGCCCAGC CTAGAACCCC CAAGTGTGGC CTCGAATGCT 
AAGGCCACAG GCACTCTCAA GAGGCCAACC AGCCTGAGCC GCCATGCCAG CGCTGCTGGC 
TTCCCTCTGT CGGGGGCCAG CACTTGGACG CTGGGCCGCA GCCATCGCAG CCCACTGTCT 
GCGGCCAGCC CGGCCGAGGC ACCCATCCAG GGACCCTGCC CAGACACCGT GGAGGACATC 
TCCCACCTGC TGGCTGACGT GGCCCGCTTT GCCGAGGGTC TTGAGAAACT GAAGGAGTGT 
GTTCTGCATG ATGAACTCCT TGAGGCCCGC CGGCCACTGG CCCATGAGTG CCTGGGTGAG 
GCCCTCCGTG TGATGCACCA GGTCATCTCC AAGTACCCAC TGCTGAACAC TGTGGAGACA 
CTCACGGCTG CTGGCACCCT CATTGCCAAA GTCAAAGCCT TCCATTATGA GTGCAACAAT 
GAGTCAGACA AGCAGGAGTT TGAGAAGGCC TTGGAGACCA TTGCTGTCTC CTTTAGCAGC 
ACTGTATCCG AGTTCCTCAT GGGTGAAGTG GACAGCAGCA TCCTCCTGTC CGTGCCCCCT 
GGGGACCCAG GCCAGTCCAT GGAGAACCTG TATGGACAGG CGAGTGAGAG TGCCCCCCCC 
AGCGGTGAGG AGTGTGATGC GGGCTGCCTG TCCCCGGAGG ACGTGGACAC CCTGCTGCAG 
CGCTGTGAGG GGGGCGTGGA CGCTGCCCTG CAGTACGCCA AGAACATGGC CAAATACATG 
AAGGACCTCA TCGGCTACCT GGAGAAGCGG AGTGCGCTGG AGATGGACTT TGCCAAAGGC 
CTGCAGAAGA TCGTCCAGAA CTGCAGACAG AGTGTCATGC AGGAGCCGCA CATGCCCCTC 
CTGTCCATCT ACTCACTGGC GCTGGAACAG GACCTGGAGT TCGGCCATGG CCTGGTGCAG 
GCAGTGGGCA CGCTGCTGAC CCAGACCTTC ATGCAGCCCC TGAATCTGCG GCGGCTCGAA 
CATGAGAAGC GAAGGAAGGA GATCAAAGAG TCCTGGCACC GCGCCCAGAG GAAGCTGCAA 
GAGGCAGAGT CCAATCTCCG CAAGGCCAAG CAGGGCTACA TGCAGCGCTG CGAGGACCAT 
GACAAGGCCC GCTTCCTGGT GGCCAAGGCG GAGGAGGAGC AAGCCAACAT TGGGCCCGGT 
GCAGGGGGTG CAGCCTCCAA GACCCTGGAC AAGCGTCGTC GCCTGGAGGA GGAGGCCAAG 
AACAAGGCGG AGGAAGCAAT GGCCACCTAC CGTACATGCG TGGCCGATGC TAAGACACAG 
AAGCAGGAGC TGGAGGATAC CAAGGTGACG GCACTACGGC AGATCCAGGA GGTCATCCGG 
CAGAGTGACC AGACCATCAA GTCGGCCACC ATCTCATATT ACCAGATGAT GCACACGCAG 
ACGGCCCCAC TGCCCGTGCA CTTCCACATG CTGTATGAGA GCAGCAAGCT GTACGACCCA 
GGCCAGCAGT ACGCTTCCCA TGTGCGCCAG CTGCAGCGCG GCGAGGAGCC TGATGTGCAC 
TACGACTTCG AGCCCCACGT GTCCGCCAGT GCCTGGTCCC CAGTCCTGCG TGCTCGGAAA 
AGCAGCTTCA ATGTCAGCGA TGCTGTGGGG GCAGAGGCTG CCAGTAGCCC CCCGGAGGAC 
GGCGGGCCCA GCCAGGGAGA GATTGCCAAG GAGCGCAGGG GTGGGCGGGG CCACCAGGTG 
CACAAGTCGT GGCCCATCTC CATCTCCGAT TCGGAGGCCA GTCTGGACCC CAGCCCTGGC 
TCAGAGGACT TTAAGAAGTT TGAGCGTATG TCTTCCTGTG GCACCATGTC GTCCAACGAG 
GAGCTGGCCG ACCAGGAGGG CAGTGCAGGG GCATCAGCCT TTGATCAGGC TGACCTCAAT 
GGCATGACTC CAGAGCTGCC TGTGGCTGTG CCCAGCGGGC CCTTCCGCCA CGTGGGGCTG 
TCCAAGGCGG CCCGGACCCA CCGGCTTCGG AAGCTCCGCA CACCGGCCAA GTGCCGGGAA 
TGCAACAGCT ACGTGTACTT CCAGGGCGCC GAGTGTGAGG AGTGCTGCCT GGCCTGCCAC 
AAGAAGTGCC TGGAGACCCT GGCCATCCAG TGTGGCCACA AGAAGCTGCA GGGCCGTCTG 
CAGCTCTTTG GCCAGGACTT CAGCCGTGCT GCCTGCAGCA CCCCCGACGG CGTGCCCTTC 
ATCATCAAGA AGTGCATCTT TGAGATCGAG CAGCGGGCGC TGCGCACCAA GGGCATCTAC 
CGGGTCAATG GGGTGAAGAC ACGTGTAGAG AAGCTGTGCC AGGCCTTCGA GAATGGCAAG 
GAGCTGGTGG AATTGTCGCA AGCCTCACCC CACGACATCA GCAATGTCCT CAAGCTCTAT 
CTGCGCCAGC TCCCTGAGCC GCTCATTTCC TTCCGCTTCT ACCATGAGCT TGTGGGGATG 
GCCAAAGACA GTCTGAAGGC CGAGGCCGAG GCCAAGGCAG CATCTCGGGG TCGGCCGGAT 
GCCACAGAGA GGGAGGCTGC AGCCATGGCC ATGGCGAGCC GGTTGCGGGA ACTCCTACGG 
GACCTGCCTC GGGATAACTG GGCCACACTG CGGTACCTGA TGCGGCACCT GCGCAGGATC 
GTGGAGGTGG AACAGGACAA CAAGATGACA CCAGGGAACC TGGGCATTGT GTTTGGGCCC 
ACGCTGCTGC GGCCCCGGCC CACTGAGGCC ACGGTGTCCC TTTCCTCCCT GGTGGACTAC 
CCCCACCAGG CCTGCATCGT GGAGACCCTC ATCACCCACT TCAGCCTGGT CTTCAAGGAG 
GAGCCCGAAG AGGCCCCAGG GGGCCAGGAT GGGGTATCCA GCCAGCGACC CGAGGTGGTG 
GTCCAGGCAC CGTACCTGGG GGGCAGCGGG GGTGCAGCCT TTCCCTTGAC AGAGGAGGCT 
GAGGATGGGG TCCTTGAACC CCATGTCGCC TCCAATGACT CCGACTCAGA GCTGGAGGAG 
GCCTCAGACC TGCAGTCCCC GGTGGGTGGG GCTGCACTGC ACCGGCTCAG TTTTCTGGAG 
AGGCAGGGCG GCGAGGCAGG CACAGAGGGC AGTCAGGGCA GCCGCAGCGG CAGCGAAGAG 
CAGCTGGGTG CCACAGCCGG CGAGTACGGG CCTGGGGCCT GGGAGGGCCC TGGGGAGGAG 
CCAGCCCGGA GGCTCTCCGA GTACAACACC AACCAGTGCA ACAACACCAG CCAGTGCACC 
ACCACCACCC AGTGCAACGC CACCAACCGG TGCAACAACG TGGCTGCAGC TTGGCTGCCA 
GCTGTGAGGC TGCGTGGTGG GCGGCTGGTA GGGGGCAACA GCTGCAAGAG GCAGCCAGAG 
TTTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( XM_024994153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849921.1 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X2, mRNA.

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CloneID	OBa235994
Clone ID Related Accession (Same CDS sequence)	XM_024994153.1
Accession Version	XM_024994153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAGCATC CTCCTGTCCG TGCCCCCTGG GGACCCAGGC 
CAGTCCATGG AGAACCTGTA TGGACAGGCG AGTGAGAGTG CCCCCCCCAG CGGTGAGGAG 
TGTGATGCGG GCTGCCTGTC CCCGGAGGAC GTGGACACCC TGCTGCAGCG CTGTGAGGGG 
GGCGTGGACG CTGCCCTGCA GTACGCCAAG AACATGGCCA AATACATGAA GGACCTCATC 
GGCTACCTGG AGAAGCGGAG TGCGCTGGAG ATGGACTTTG CCAAAGGCCT GCAGAAGATC 
GTCCAGAACT GCAGACAGAG TGTCATGCAG GAGCCGCACA TGCCCCTCCT GTCCATCTAC 
TCACTGGCGC TGGAACAGGA CCTGGAGTTC GGCCATGGCC TGGTGCAGGC AGTGGGCACG 
CTGCTGACCC AGACCTTCAT GCAGCCCCTG AATCTGCGGC GGCTCGAACA TGAGAAGCGA 
AGGAAGGAGA TCAAAGAGTC CTGGCACCGC GCCCAGAGGA AGCTGCAAGA GGCAGAGTCC 
AATCTCCGCA AGGCCAAGCA GGGCTACATG CAGCGCTGCG AGGACCATGA CAAGGCCCGC 
TTCCTGGTGG CCAAGGCGGA GGAGGAGCAA GCCAACATTG GGCCCGGTGC AGGGGGTGCA 
GCCTCCAAGA CCCTGGACAA GCGTCGTCGC CTGGAGGAGG AGGCCAAGAA CAAGGCGGAG 
GAAGCAATGG CCACCTACCG TACATGCGTG GCCGATGCTA AGACACAGAA GCAGGAGCTG 
GAGGATACCA AGGTGACGGC ACTACGGCAG ATCCAGGAGG TCATCCGGCA GAGTGACCAG 
ACCATCAAGT CGGCCACCAT CTCATATTAC CAGATGATGC ACACGCAGAC GGCCCCACTG 
CCCGTGCACT TCCACATGCT GTATGAGAGC AGCAAGCTGT ACGACCCAGG CCAGCAGTAC 
GCTTCCCATG TGCGCCAGCT GCAGCGCGGC GAGGAGCCTG ATGTGCACTA CGACTTCGAG 
CCCCACGTGT CCGCCAGTGC CTGGTCCCCA GTCCTGCGTG CTCGGAAAAG CAGCTTCAAT 
GTCAGCGATG CTGTGGGGGC AGAGGCTGCC AGTAGCCCCC CGGAGGACGG CGGGCCCAGC 
CAGGGAGAGA TTGCCAAGGA GCGCAGGGGT GGGCGGGGCC ACCAGGTGCA CAAGTCGTGG 
CCCATCTCCA TCTCCGATTC GGAGGCCAGT CTGGACCCCA GCCCTGGCTC AGAGGACTTT 
AAGAAGTTTG AGCGTATGTC TTCCTGTGGC ACCATGTCGT CCAACGAGGA GCTGGCCGAC 
CAGGAGGGCA GTGCAGGGGC ATCAGCCTTT GATCAGGCTG ACCTCAATGG CATGACTCCA 
GAGCTGCCTG TGGCTGTGCC CAGCGGGCCC TTCCGCCACG TGGGGCTGTC CAAGGCGGCC 
CGGACCCACC GGCTTCGGAA GCTCCGCACA CCGGCCAAGT GCCGGGAATG CAACAGCTAC 
GTGTACTTCC AGGGCGCCGA GTGTGAGGAG TGCTGCCTGG CCTGCCACAA GAAGTGCCTG 
GAGACCCTGG CCATCCAGTG TGGCCACAAG AAGCTGCAGG GCCGTCTGCA GCTCTTTGGC 
CAGGACTTCA GCCGTGCTGC CTGCAGCACC CCCGACGGCG TGCCCTTCAT CATCAAGAAG 
TGCATCTTTG AGATCGAGCA GCGGGCGCTG CGCACCAAGG GCATCTACCG GGTCAATGGG 
GTGAAGACAC GTGTAGAGAA GCTGTGCCAG GCCTTCGAGA ATGGCAAGGA GCTGGTGGAA 
TTGTCGCAAG CCTCACCCCA CGACATCAGC AATGTCCTCA AGCTCTATCT GCGCCAGCTC 
CCTGAGCCGC TCATTTCCTT CCGCTTCTAC CATGAGCTTG TGGGGATGGC CAAAGACAGT 
CTGAAGGCCG AGGCCGAGGC CAAGGCAGCA TCTCGGGGTC GGCCGGATGC CACAGAGAGG 
GAGGCTGCAG CCATGGCCAT GGCGAGCCGG TTGCGGGAAC TCCTACGGGA CCTGCCTCGG 
GATAACTGGG CCACACTGCG GTACCTGATG CGGCACCTGC GCAGGATCGT GGAGGTGGAA 
CAGGACAACA AGATGACACC AGGGAACCTG GGCATTGTGT TTGGGCCCAC GCTGCTGCGG 
CCCCGGCCCA CTGAGGCCAC GGTGTCCCTT TCCTCCCTGG TGGACTACCC CCACCAGGCC 
TGCATCGTGG AGACCCTCAT CACCCACTTC AGCCTGGTCT TCAAGGAGGA GCCCGAAGAG 
GCCCCAGGGG GCCAGGATGG GGTATCCAGC CAGCGACCCG AGGTGGTGGT CCAGGCACCG 
TACCTGGGGG GCAGCGGGGG TGCAGCCTTT CCCTTGACAG AGGAGGCTGA GGATGGGGTC 
CTTGAACCCC ATGTCGCCTC CAATGACTCC GACTCAGAGC TGGAGGAGGC CTCAGACCTG 
CAGTCCCCGG TGGGTGGGGC TGCACTGCAC CGGCTCAGTT TTCTGGAGAG GCAGGGCGGC 
GAGGCAGGCA CAGAGGGCAG TCAGGGCAGC CGCAGCGGCA GCGAAGAGCA GCTGGGTGCC 
ACAGCCGGCG AGTACGGGCC TGGGGCCTGG GAGGGCCCTG GGGAGGAGCC AGCCCGGAGG 
CTCTCCGAGT ACAACACCAA CCAGTGCAAC AACACCAGCC AGTGCACCAC CACCACCCAG 
TGCAACGCCA CCAACCGGTG CAACAACGTG GCTGCAGCTT GGCTGCCAGC TGTGAGGCTG 
CGTGGTGGGC GGCTGGTAGG GGGCAACAGC TGCAAGAGGC AGCCAGAGTT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849921.1
CDS	147..3623
Translation	MFSRKKRELMKTPSISKKNRAGSPCPQPSGELPRRDGADAVSLGPSLEPPSVASNAKATGTLKRPTSLSRHASAAGFPLSGASTWTLGRSHRSPLSAASPAEAPIQGPCPDTVEDISHLLADVARFAEGLEKLKECVLHDELLEARRPLAHECLGEALRVMHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSSILLSVPPGDPGQSMENLYGQASESAPPSGEECDAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQCTTTTQCNATNRCNNVAAAWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	XM_024994153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994153.1

ORF Insert Sequence:

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAGCATC CTCCTGTCCG TGCCCCCTGG GGACCCAGGC 
CAGTCCATGG AGAACCTGTA TGGACAGGCG AGTGAGAGTG CCCCCCCCAG CGGTGAGGAG 
TGTGATGCGG GCTGCCTGTC CCCGGAGGAC GTGGACACCC TGCTGCAGCG CTGTGAGGGG 
GGCGTGGACG CTGCCCTGCA GTACGCCAAG AACATGGCCA AATACATGAA GGACCTCATC 
GGCTACCTGG AGAAGCGGAG TGCGCTGGAG ATGGACTTTG CCAAAGGCCT GCAGAAGATC 
GTCCAGAACT GCAGACAGAG TGTCATGCAG GAGCCGCACA TGCCCCTCCT GTCCATCTAC 
TCACTGGCGC TGGAACAGGA CCTGGAGTTC GGCCATGGCC TGGTGCAGGC AGTGGGCACG 
CTGCTGACCC AGACCTTCAT GCAGCCCCTG AATCTGCGGC GGCTCGAACA TGAGAAGCGA 
AGGAAGGAGA TCAAAGAGTC CTGGCACCGC GCCCAGAGGA AGCTGCAAGA GGCAGAGTCC 
AATCTCCGCA AGGCCAAGCA GGGCTACATG CAGCGCTGCG AGGACCATGA CAAGGCCCGC 
TTCCTGGTGG CCAAGGCGGA GGAGGAGCAA GCCAACATTG GGCCCGGTGC AGGGGGTGCA 
GCCTCCAAGA CCCTGGACAA GCGTCGTCGC CTGGAGGAGG AGGCCAAGAA CAAGGCGGAG 
GAAGCAATGG CCACCTACCG TACATGCGTG GCCGATGCTA AGACACAGAA GCAGGAGCTG 
GAGGATACCA AGGTGACGGC ACTACGGCAG ATCCAGGAGG TCATCCGGCA GAGTGACCAG 
ACCATCAAGT CGGCCACCAT CTCATATTAC CAGATGATGC ACACGCAGAC GGCCCCACTG 
CCCGTGCACT TCCACATGCT GTATGAGAGC AGCAAGCTGT ACGACCCAGG CCAGCAGTAC 
GCTTCCCATG TGCGCCAGCT GCAGCGCGGC GAGGAGCCTG ATGTGCACTA CGACTTCGAG 
CCCCACGTGT CCGCCAGTGC CTGGTCCCCA GTCCTGCGTG CTCGGAAAAG CAGCTTCAAT 
GTCAGCGATG CTGTGGGGGC AGAGGCTGCC AGTAGCCCCC CGGAGGACGG CGGGCCCAGC 
CAGGGAGAGA TTGCCAAGGA GCGCAGGGGT GGGCGGGGCC ACCAGGTGCA CAAGTCGTGG 
CCCATCTCCA TCTCCGATTC GGAGGCCAGT CTGGACCCCA GCCCTGGCTC AGAGGACTTT 
AAGAAGTTTG AGCGTATGTC TTCCTGTGGC ACCATGTCGT CCAACGAGGA GCTGGCCGAC 
CAGGAGGGCA GTGCAGGGGC ATCAGCCTTT GATCAGGCTG ACCTCAATGG CATGACTCCA 
GAGCTGCCTG TGGCTGTGCC CAGCGGGCCC TTCCGCCACG TGGGGCTGTC CAAGGCGGCC 
CGGACCCACC GGCTTCGGAA GCTCCGCACA CCGGCCAAGT GCCGGGAATG CAACAGCTAC 
GTGTACTTCC AGGGCGCCGA GTGTGAGGAG TGCTGCCTGG CCTGCCACAA GAAGTGCCTG 
GAGACCCTGG CCATCCAGTG TGGCCACAAG AAGCTGCAGG GCCGTCTGCA GCTCTTTGGC 
CAGGACTTCA GCCGTGCTGC CTGCAGCACC CCCGACGGCG TGCCCTTCAT CATCAAGAAG 
TGCATCTTTG AGATCGAGCA GCGGGCGCTG CGCACCAAGG GCATCTACCG GGTCAATGGG 
GTGAAGACAC GTGTAGAGAA GCTGTGCCAG GCCTTCGAGA ATGGCAAGGA GCTGGTGGAA 
TTGTCGCAAG CCTCACCCCA CGACATCAGC AATGTCCTCA AGCTCTATCT GCGCCAGCTC 
CCTGAGCCGC TCATTTCCTT CCGCTTCTAC CATGAGCTTG TGGGGATGGC CAAAGACAGT 
CTGAAGGCCG AGGCCGAGGC CAAGGCAGCA TCTCGGGGTC GGCCGGATGC CACAGAGAGG 
GAGGCTGCAG CCATGGCCAT GGCGAGCCGG TTGCGGGAAC TCCTACGGGA CCTGCCTCGG 
GATAACTGGG CCACACTGCG GTACCTGATG CGGCACCTGC GCAGGATCGT GGAGGTGGAA 
CAGGACAACA AGATGACACC AGGGAACCTG GGCATTGTGT TTGGGCCCAC GCTGCTGCGG 
CCCCGGCCCA CTGAGGCCAC GGTGTCCCTT TCCTCCCTGG TGGACTACCC CCACCAGGCC 
TGCATCGTGG AGACCCTCAT CACCCACTTC AGCCTGGTCT TCAAGGAGGA GCCCGAAGAG 
GCCCCAGGGG GCCAGGATGG GGTATCCAGC CAGCGACCCG AGGTGGTGGT CCAGGCACCG 
TACCTGGGGG GCAGCGGGGG TGCAGCCTTT CCCTTGACAG AGGAGGCTGA GGATGGGGTC 
CTTGAACCCC ATGTCGCCTC CAATGACTCC GACTCAGAGC TGGAGGAGGC CTCAGACCTG 
CAGTCCCCGG TGGGTGGGGC TGCACTGCAC CGGCTCAGTT TTCTGGAGAG GCAGGGCGGC 
GAGGCAGGCA CAGAGGGCAG TCAGGGCAGC CGCAGCGGCA GCGAAGAGCA GCTGGGTGCC 
ACAGCCGGCG AGTACGGGCC TGGGGCCTGG GAGGGCCCTG GGGAGGAGCC AGCCCGGAGG 
CTCTCCGAGT ACAACACCAA CCAGTGCAAC AACACCAGCC AGTGCACCAC CACCACCCAG 
TGCAACGCCA CCAACCGGTG CAACAACGTG GCTGCAGCTT GGCTGCCAGC TGTGAGGCTG 
CGTGGTGGGC GGCTGGTAGG GGGCAACAGC TGCAAGAGGC AGCCAGAGTT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( XM_024994154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849922.1 Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X3, mRNA.

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CloneID	OBa235995
Clone ID Related Accession (Same CDS sequence)	XM_024994154.1
Accession Version	XM_024994154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 45 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATCT GCGGGACGGT GCACCCCGCG CTGGACCAGA GGCCTGTGCG CTGCCAGGCA 
GGGCCCCGAG AACTCCTTGA GGCCCGCCGG CCACTGGCCC ATGAGTGCCT GGGTGAGGCC 
CTCCGTGTGA TGCACCAGGT CATCTCCAAG TACCCACTGC TGAACACTGT GGAGACACTC 
ACGGCTGCTG GCACCCTCAT TGCCAAAGTC AAAGCCTTCC ATTATGAGTG CAACAATGAG 
TCAGACAAGC AGGAGTTTGA GAAGGCCTTG GAGACCATTG CTGTCTCCTT TAGCAGCACT 
GTATCCGAGT TCCTCATGGG TGAAGTGGAC AGCAGCATCC TCCTGTCCGT GCCCCCTGGG 
GACCCAGGCC AGTCCATGGA GAACCTGTAT GGACAGGCGA GTGAGAGTGC CCCCCCCAGC 
GGTGAGGAGT GTGATGCGGG CTGCCTGTCC CCGGAGGACG TGGACACCCT GCTGCAGCGC 
TGTGAGGGGG GCGTGGACGC TGCCCTGCAG TACGCCAAGA ACATGGCCAA ATACATGAAG 
GACCTCATCG GCTACCTGGA GAAGCGGAGT GCGCTGGAGA TGGACTTTGC CAAAGGCCTG 
CAGAAGATCG TCCAGAACTG CAGACAGAGT GTCATGCAGG AGCCGCACAT GCCCCTCCTG 
TCCATCTACT CACTGGCGCT GGAACAGGAC CTGGAGTTCG GCCATGGCCT GGTGCAGGCA 
GTGGGCACGC TGCTGACCCA GACCTTCATG CAGCCCCTGA ATCTGCGGCG GCTCGAACAT 
GAGAAGCGAA GGAAGGAGAT CAAAGAGTCC TGGCACCGCG CCCAGAGGAA GCTGCAAGAG 
GCAGAGTCCA ATCTCCGCAA GGCCAAGCAG GGCTACATGC AGCGCTGCGA GGACCATGAC 
AAGGCCCGCT TCCTGGTGGC CAAGGCGGAG GAGGAGCAAG CCAACATTGG GCCCGGTGCA 
GGGGGTGCAG CCTCCAAGAC CCTGGACAAG CGTCGTCGCC TGGAGGAGGA GGCCAAGAAC 
AAGGCGGAGG AAGCAATGGC CACCTACCGT ACATGCGTGG CCGATGCTAA GACACAGAAG 
CAGGAGCTGG AGGATACCAA GGTGACGGCA CTACGGCAGA TCCAGGAGGT CATCCGGCAG 
AGTGACCAGA CCATCAAGTC GGCCACCATC TCATATTACC AGATGATGCA CACGCAGACG 
GCCCCACTGC CCGTGCACTT CCACATGCTG TATGAGAGCA GCAAGCTGTA CGACCCAGGC 
CAGCAGTACG CTTCCCATGT GCGCCAGCTG CAGCGCGGCG AGGAGCCTGA TGTGCACTAC 
GACTTCGAGC CCCACGTGTC CGCCAGTGCC TGGTCCCCAG TCCTGCGTGC TCGGAAAAGC 
AGCTTCAATG TCAGCGATGC TGTGGGGGCA GAGGCTGCCA GTAGCCCCCC GGAGGACGGC 
GGGCCCAGCC AGGGAGAGAT TGCCAAGGAG CGCAGGGGTG GGCGGGGCCA CCAGGTGCAC 
AAGTCGTGGC CCATCTCCAT CTCCGATTCG GAGGCCAGTC TGGACCCCAG CCCTGGCTCA 
GAGGACTTTA AGAAGTTTGA GCGTATGTCT TCCTGTGGCA CCATGTCGTC CAACGAGGAG 
CTGGCCGACC AGGAGGGCAG TGCAGGGGCA TCAGCCTTTG ATCAGGCTGA CCTCAATGGC 
ATGACTCCAG AGCTGCCTGT GGCTGTGCCC AGCGGGCCCT TCCGCCACGT GGGGCTGTCC 
AAGGCGGCCC GGACCCACCG GCTTCGGAAG CTCCGCACAC CGGCCAAGTG CCGGGAATGC 
AACAGCTACG TGTACTTCCA GGGCGCCGAG TGTGAGGAGT GCTGCCTGGC CTGCCACAAG 
AAGTGCCTGG AGACCCTGGC CATCCAGTGT GGCCACAAGA AGCTGCAGGG CCGTCTGCAG 
CTCTTTGGCC AGGACTTCAG CCGTGCTGCC TGCAGCACCC CCGACGGCGT GCCCTTCATC 
ATCAAGAAGT GCATCTTTGA GATCGAGCAG CGGGCGCTGC GCACCAAGGG CATCTACCGG 
GTCAATGGGG TGAAGACACG TGTAGAGAAG CTGTGCCAGG CCTTCGAGAA TGGCAAGGAG 
CTGGTGGAAT TGTCGCAAGC CTCACCCCAC GACATCAGCA ATGTCCTCAA GCTCTATCTG 
CGCCAGCTCC CTGAGCCGCT CATTTCCTTC CGCTTCTACC ATGAGCTTGT GGGGATGGCC 
AAAGACAGTC TGAAGGCCGA GGCCGAGGCC AAGGCAGCAT CTCGGGGTCG GCCGGATGCC 
ACAGAGAGGG AGGCTGCAGC CATGGCCATG GCGAGCCGGT TGCGGGAACT CCTACGGGAC 
CTGCCTCGGG ATAACTGGGC CACACTGCGG TACCTGATGC GGCACCTGCG CAGGATCGTG 
GAGGTGGAAC AGGACAACAA GATGACACCA GGGAACCTGG GCATTGTGTT TGGGCCCACG 
CTGCTGCGGC CCCGGCCCAC TGAGGCCACG GTGTCCCTTT CCTCCCTGGT GGACTACCCC 
CACCAGGCCT GCATCGTGGA GACCCTCATC ACCCACTTCA GCCTGGTCTT CAAGGAGGAG 
CCCGAAGAGG CCCCAGGGGG CCAGGATGGG GTATCCAGCC AGCGACCCGA GGTGGTGGTC 
CAGGCACCGT ACCTGGGGGG CAGCGGGGGT GCAGCCTTTC CCTTGACAGA GGAGGCTGAG 
GATGGGGTCC TTGAACCCCA TGTCGCCTCC AATGACTCCG ACTCAGAGCT GGAGGAGGCC 
TCAGACCTGC AGTCCCCGGT GGGTGGGGCT GCACTGCACC GGCTCAGTTT TCTGGAGAGG 
CAGGGCGGCG AGGCAGGCAC AGAGGGCAGT CAGGGCAGCC GCAGCGGCAG CGAAGAGCAG 
CTGGGTGCCA CAGCCGGCGA GTACGGGCCT GGGGCCTGGG AGGGCCCTGG GGAGGAGCCA 
GCCCGGAGGC TCTCCGAGTA CAACACCAAC CAGTGCAACA ACACCAGCCA GTGCACCACC 
ACCACCCAGT GCAACGCCAC CAACCGGTGC AACAACGTGG CTGCAGCTTG GCTGCCAGCT 
GTGAGGCTGC GTGGTGGGCG GCTGGTAGGG GGCAACAGCT GCAAGAGGCA GCCAGAGTTT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849922.1
CDS	1523..4648
Translation	MCICGTVHPALDQRPVRCQAGPRELLEARRPLAHECLGEALRVMHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSSILLSVPPGDPGQSMENLYGQASESAPPSGEECDAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQCTTTTQCNATNRCNNVAAAWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	XM_024994154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 45 (ARHGAP45), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994154.1

ORF Insert Sequence:

ATGTGTATCT GCGGGACGGT GCACCCCGCG CTGGACCAGA GGCCTGTGCG CTGCCAGGCA 
GGGCCCCGAG AACTCCTTGA GGCCCGCCGG CCACTGGCCC ATGAGTGCCT GGGTGAGGCC 
CTCCGTGTGA TGCACCAGGT CATCTCCAAG TACCCACTGC TGAACACTGT GGAGACACTC 
ACGGCTGCTG GCACCCTCAT TGCCAAAGTC AAAGCCTTCC ATTATGAGTG CAACAATGAG 
TCAGACAAGC AGGAGTTTGA GAAGGCCTTG GAGACCATTG CTGTCTCCTT TAGCAGCACT 
GTATCCGAGT TCCTCATGGG TGAAGTGGAC AGCAGCATCC TCCTGTCCGT GCCCCCTGGG 
GACCCAGGCC AGTCCATGGA GAACCTGTAT GGACAGGCGA GTGAGAGTGC CCCCCCCAGC 
GGTGAGGAGT GTGATGCGGG CTGCCTGTCC CCGGAGGACG TGGACACCCT GCTGCAGCGC 
TGTGAGGGGG GCGTGGACGC TGCCCTGCAG TACGCCAAGA ACATGGCCAA ATACATGAAG 
GACCTCATCG GCTACCTGGA GAAGCGGAGT GCGCTGGAGA TGGACTTTGC CAAAGGCCTG 
CAGAAGATCG TCCAGAACTG CAGACAGAGT GTCATGCAGG AGCCGCACAT GCCCCTCCTG 
TCCATCTACT CACTGGCGCT GGAACAGGAC CTGGAGTTCG GCCATGGCCT GGTGCAGGCA 
GTGGGCACGC TGCTGACCCA GACCTTCATG CAGCCCCTGA ATCTGCGGCG GCTCGAACAT 
GAGAAGCGAA GGAAGGAGAT CAAAGAGTCC TGGCACCGCG CCCAGAGGAA GCTGCAAGAG 
GCAGAGTCCA ATCTCCGCAA GGCCAAGCAG GGCTACATGC AGCGCTGCGA GGACCATGAC 
AAGGCCCGCT TCCTGGTGGC CAAGGCGGAG GAGGAGCAAG CCAACATTGG GCCCGGTGCA 
GGGGGTGCAG CCTCCAAGAC CCTGGACAAG CGTCGTCGCC TGGAGGAGGA GGCCAAGAAC 
AAGGCGGAGG AAGCAATGGC CACCTACCGT ACATGCGTGG CCGATGCTAA GACACAGAAG 
CAGGAGCTGG AGGATACCAA GGTGACGGCA CTACGGCAGA TCCAGGAGGT CATCCGGCAG 
AGTGACCAGA CCATCAAGTC GGCCACCATC TCATATTACC AGATGATGCA CACGCAGACG 
GCCCCACTGC CCGTGCACTT CCACATGCTG TATGAGAGCA GCAAGCTGTA CGACCCAGGC 
CAGCAGTACG CTTCCCATGT GCGCCAGCTG CAGCGCGGCG AGGAGCCTGA TGTGCACTAC 
GACTTCGAGC CCCACGTGTC CGCCAGTGCC TGGTCCCCAG TCCTGCGTGC TCGGAAAAGC 
AGCTTCAATG TCAGCGATGC TGTGGGGGCA GAGGCTGCCA GTAGCCCCCC GGAGGACGGC 
GGGCCCAGCC AGGGAGAGAT TGCCAAGGAG CGCAGGGGTG GGCGGGGCCA CCAGGTGCAC 
AAGTCGTGGC CCATCTCCAT CTCCGATTCG GAGGCCAGTC TGGACCCCAG CCCTGGCTCA 
GAGGACTTTA AGAAGTTTGA GCGTATGTCT TCCTGTGGCA CCATGTCGTC CAACGAGGAG 
CTGGCCGACC AGGAGGGCAG TGCAGGGGCA TCAGCCTTTG ATCAGGCTGA CCTCAATGGC 
ATGACTCCAG AGCTGCCTGT GGCTGTGCCC AGCGGGCCCT TCCGCCACGT GGGGCTGTCC 
AAGGCGGCCC GGACCCACCG GCTTCGGAAG CTCCGCACAC CGGCCAAGTG CCGGGAATGC 
AACAGCTACG TGTACTTCCA GGGCGCCGAG TGTGAGGAGT GCTGCCTGGC CTGCCACAAG 
AAGTGCCTGG AGACCCTGGC CATCCAGTGT GGCCACAAGA AGCTGCAGGG CCGTCTGCAG 
CTCTTTGGCC AGGACTTCAG CCGTGCTGCC TGCAGCACCC CCGACGGCGT GCCCTTCATC 
ATCAAGAAGT GCATCTTTGA GATCGAGCAG CGGGCGCTGC GCACCAAGGG CATCTACCGG 
GTCAATGGGG TGAAGACACG TGTAGAGAAG CTGTGCCAGG CCTTCGAGAA TGGCAAGGAG 
CTGGTGGAAT TGTCGCAAGC CTCACCCCAC GACATCAGCA ATGTCCTCAA GCTCTATCTG 
CGCCAGCTCC CTGAGCCGCT CATTTCCTTC CGCTTCTACC ATGAGCTTGT GGGGATGGCC 
AAAGACAGTC TGAAGGCCGA GGCCGAGGCC AAGGCAGCAT CTCGGGGTCG GCCGGATGCC 
ACAGAGAGGG AGGCTGCAGC CATGGCCATG GCGAGCCGGT TGCGGGAACT CCTACGGGAC 
CTGCCTCGGG ATAACTGGGC CACACTGCGG TACCTGATGC GGCACCTGCG CAGGATCGTG 
GAGGTGGAAC AGGACAACAA GATGACACCA GGGAACCTGG GCATTGTGTT TGGGCCCACG 
CTGCTGCGGC CCCGGCCCAC TGAGGCCACG GTGTCCCTTT CCTCCCTGGT GGACTACCCC 
CACCAGGCCT GCATCGTGGA GACCCTCATC ACCCACTTCA GCCTGGTCTT CAAGGAGGAG 
CCCGAAGAGG CCCCAGGGGG CCAGGATGGG GTATCCAGCC AGCGACCCGA GGTGGTGGTC 
CAGGCACCGT ACCTGGGGGG CAGCGGGGGT GCAGCCTTTC CCTTGACAGA GGAGGCTGAG 
GATGGGGTCC TTGAACCCCA TGTCGCCTCC AATGACTCCG ACTCAGAGCT GGAGGAGGCC 
TCAGACCTGC AGTCCCCGGT GGGTGGGGCT GCACTGCACC GGCTCAGTTT TCTGGAGAGG 
CAGGGCGGCG AGGCAGGCAC AGAGGGCAGT CAGGGCAGCC GCAGCGGCAG CGAAGAGCAG 
CTGGGTGCCA CAGCCGGCGA GTACGGGCCT GGGGCCTGGG AGGGCCCTGG GGAGGAGCCA 
GCCCGGAGGC TCTCCGAGTA CAACACCAAC CAGTGCAACA ACACCAGCCA GTGCACCACC 
ACCACCCAGT GCAACGCCAC CAACCGGTGC AACAACGTGG CTGCAGCTTG GCTGCCAGCT 
GTGAGGCTGC GTGGTGGGCG GCTGGTAGGG GGCAACAGCT GCAAGAGGCA GCCAGAGTTT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP45 ( NM_001205741.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192670.1 Bos taurus histocompatibility (minor) HA-1 (HMHA1), mRNA.

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CloneID	OBa39309
Clone ID Related Accession (Same CDS sequence)	NM_001205741.1
Accession Version	NM_001205741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	minor histocompatibility protein HA-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03117014.1, AAFC03096909.1 and AAFC03096918.1. On Apr 20, 2011 this sequence version replaced XM_590584.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01093728, SAMN01093729 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAACTGG GTTGTGGATG GGAGGAGGGG TGGGCTGGGG 
CAGGGGAGGG CTGGGCAGGG TGAGCCTGGG CTGAGGGGTG TTTACTCTGC AGGCTGCCTG 
TCCCCGGAGG ACGTGGACAC CCTGCTGCAG CGCTGTGAGG GGGGCGTGGA CGCTGCCCTG 
CAGTACGCCA AGAACATGGC CAAATACATG AAGGACCTCA TCGGCTACCT GGAGAAGCGG 
AGTGCGCTGG AGATGGACTT TGCCAAAGGC CTGCAGAAGA TCGTCCAGAA CTGCAGACAG 
AGTGTCATGC AGGAGCCGCA CATGCCCCTC CTGTCCATCT ACTCACTGGC GCTGGAACAG 
GACCTGGAGT TCGGCCATGG CCTGGTGCAG GCAGTGGGCA CGCTGCTGAC CCAGACCTTC 
ATGCAGCCCC TGAATCTGCG GCGGCTCGAA CATGAGAAGC GAAGGAAGGA GATCAAAGAG 
TCCTGGCACC GCGCCCAGAG GAAGCTGCAA GAGGCAGAGT CCAATCTCCG CAAGGCCAAG 
CAGGGCTACA TGCAGCGCTG CGAGGACCAT GACAAGGCCC GCTTCCTGGT GGCCAAGGCG 
GAGGAGGAGC AAGCCAACAT TGGGCCCGGT GCAGGGGGTG CAGCCTCCAA GACCCTGGAC 
AAGCGTCGTC GCCTGGAGGA GGAGGCCAAG AACAAGGCGG AGGAAGCAAT GGCCACCTAC 
CGTACATGCG TGGCCGATGC TAAGACACAG AAGCAGGAGC TGGAGGATAC CAAGGTGACG 
GCACTACGGC AGATCCAGGA GGTCATCCGG CAGAGTGACC AGACCATCAA GTCGGCCACC 
ATCTCATATT ACCAGATGAT GCACACGCAG ACGGCCCCAC TGCCCGTGCA CTTCCACATG 
CTGTATGAGA GCAGCAAGCT GTACGACCCA GGCCAGCAGT ACGCTTCCCA TGTGCGCCAG 
CTGCAGCGCG GCGAGGAGCC TGATGTGCAC TACGACTTCG AGCCCCACGT GTCCGCCAGT 
GCCTGGTCCC CAGTCCTGCG TGCTCGGAAA AGCAGCTTCA ATGTCAGCGA TGCTGTGGGG 
GCAGAGGCTG CCAGTAGCCC CCCGGAGGAC GGCGGGCCCA GCCAGGGAGA GATTGCCAAG 
GAGCGCAGGG GTGGGCGGGG CCACCAGGTG CACAAGTCGT GGCCCATCTC CATCTCCGAT 
TCGGAGGCCA GTCTGGACCC CAGCCCTGGC TCAGAGGACT TTAAGAAGTT TGAGCGTATG 
TCTTCCTGTG GCACCATGTC GTCCAACGAG GAGCTGGCCG ACCAGGAGGG CAGTGCAGGG 
GCATCAGCCT TTGATCAGGC TGACCTCAAT GGCATGACTC CAGAGCTGCC TGTGGCTGTG 
CCCAGCGGGC CCTTCCGCCA CGTGGGGCTG TCCAAGGCGG CCCGGACCCA CCGGCTTCGG 
AAGCTCCGCA CACCGGCCAA GTGCCGGGAA TGCAACAGCT ACGTGTACTT CCAGGGCGCC 
GAGTGTGAGG AGTGCTGCCT GGCCTGCCAC AAGAAGTGCC TGGAGACCCT GGCCATCCAG 
TGTGGCCACA AGAAGCTGCA GGGCCGTCTG CAGCTCTTTG GCCAGGACTT CAGCCGTGCT 
GCCTGCAGCA CCCCCGACGG CGTGCCCTTC ATCATCAAGA AGTGCATCTT TGAGATCGAG 
CAGCGGGCGC TGCGCACCAA GGGCATCTAC CGGGTCAATG GGGTGAAGAC ACGTGTAGAG 
AAGCTGTGCC AGGCCTTCGA GAATGGCAAG GAGCTGGTGG AATTGTCGCA AGCCTCACCC 
CACGACATCA GCAATGTCCT CAAGCTCTAT CTGCGCCAGC TCCCTGAGCC GCTCATTTCC 
TTCCGCTTCT ACCATGAGCT TGTGGGGATG GCCAAAGACA GTCTGAAGGC CGAGGCCGAG 
GCCAAGGCAG CATCTCGGGG TCGGCCGGAT GCCACAGAGA GGGAGGCTGC AGCCATGGCC 
ATGGCGAGCC GGTTGCGGGA ACTCCTACGG GACCTGCCTC GGGATAACTG GGCCACACTG 
CGGTACCTGA TGCGGCACCT GCGCAGGATC GTGGAGGTGG AACAGGACAA CAAGATGACA 
CCAGGGAACC TGGGCATTGT GTTTGGGCCC ACGCTGCTGC GGCCCCGGCC CACTGAGGCC 
ACGGTGTCCC TTTCCTCCCT GGTGGACTAC CCCCACCAGG CCTGCATCGT GGAGACCCTC 
ATCACCCACT TCAGCCTGGT CTTCAAGGAG GAGCCCGAAG AGGCCCCAGG GGGCCAGGAT 
GGGGTATCCA GCCAGCGACC CGAGGTGGTG GTCCAGGCAC CGTACCTGGG GGGCAGCGGG 
GGTGCAGCCT TTCCCTTGAC AGAGGAGGCT GAGGATGGGG TCCTTGAACC CCATGTCGCC 
TCCAATGACT CCGACTCAGA GCTGGAGGAG GCCTCAGACC TGCAGTCCCC GGTGGGTGGG 
GCTGCACTGC ACCGGCTCAG TTTTCTGGAG AGGCAGGGCG GCGAGGCAGG CACAGAGGGC 
AGTCAGGGCA GCCGCAGCGG CAGCGAAGAG CAGCTGGGTG CCACAGCCGG CGAGTACGGG 
CCTGGGGCCT GGGAGGGCCC TGGGGAGGAG CCAGCCCGGA GGCTCTCCGA GTACAACACC 
AACCAGTGCA ACAACACCAG CCAGTCTTGG CTGCCAGCTG TGAGGCTGCG TGGTGGGCGG 
CTGGTAGGGG GCAACAGCTG CAAGAGGCAG CCAGAGTTTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192670.1
CDS	1..3405
Translation	MFSRKKRELMKTPSISKKNRAGSPCPQPSGELPRRDGADAVSLGPSLEPPSVASNAKATGTLKRPTSLSRHASAAGFPLSGASTWTLGRSHRSPLSAASPAEAPIQGPCPDTVEDISHLLADVARFAEGLEKLKECVLHDELLEARRPLAHECLGEALRVMHQVISKYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKQEFEKALETIAVSFSSTVSEFLMGEVDSNWVVDGRRGGLGQGRAGQGEPGLRGVYSAGCLSPEDVDTLLQRCEGGVDAALQYAKNMAKYMKDLIGYLEKRSALEMDFAKGLQKIVQNCRQSVMQEPHMPLLSIYSLALEQDLEFGHGLVQAVGTLLTQTFMQPLNLRRLEHEKRRKEIKESWHRAQRKLQEAESNLRKAKQGYMQRCEDHDKARFLVAKAEEEQANIGPGAGGAASKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHTQTAPLPVHFHMLYESSKLYDPGQQYASHVRQLQRGEEPDVHYDFEPHVSASAWSPVLRARKSSFNVSDAVGAEAASSPPEDGGPSQGEIAKERRGGRGHQVHKSWPISISDSEASLDPSPGSEDFKKFERMSSCGTMSSNEELADQEGSAGASAFDQADLNGMTPELPVAVPSGPFRHVGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSRAACSTPDGVPFIIKKCIFEIEQRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGMAKDSLKAEAEAKAASRGRPDATEREAAAMAMASRLRELLRDLPRDNWATLRYLMRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQACIVETLITHFSLVFKEEPEEAPGGQDGVSSQRPEVVVQAPYLGGSGGAAFPLTEEAEDGVLEPHVASNDSDSELEEASDLQSPVGGAALHRLSFLERQGGEAGTEGSQGSRSGSEEQLGATAGEYGPGAWEGPGEEPARRLSEYNTNQCNNTSQSWLPAVRLRGGRLVGGNSCKRQPEFV

Target ORF information:

RefSeq Version	NM_001205741.1
Organism	Bos taurus(cattle)
Definition	Bos taurus histocompatibility (minor) HA-1 (HMHA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205741.1

ORF Insert Sequence:

ATGTTCTCCA GGAAGAAGAG AGAGCTCATG AAAACCCCGT CTATCTCGAA AAAGAACAGG 
GCGGGAAGCC CCTGCCCACA GCCCTCGGGG GAGCTGCCTA GGAGAGACGG GGCTGATGCA 
GTGTCCCTGG GGCCCAGCCT AGAACCCCCA AGTGTGGCCT CGAATGCTAA GGCCACAGGC 
ACTCTCAAGA GGCCAACCAG CCTGAGCCGC CATGCCAGCG CTGCTGGCTT CCCTCTGTCG 
GGGGCCAGCA CTTGGACGCT GGGCCGCAGC CATCGCAGCC CACTGTCTGC GGCCAGCCCG 
GCCGAGGCAC CCATCCAGGG ACCCTGCCCA GACACCGTGG AGGACATCTC CCACCTGCTG 
GCTGACGTGG CCCGCTTTGC CGAGGGTCTT GAGAAACTGA AGGAGTGTGT TCTGCATGAT 
GAACTCCTTG AGGCCCGCCG GCCACTGGCC CATGAGTGCC TGGGTGAGGC CCTCCGTGTG 
ATGCACCAGG TCATCTCCAA GTACCCACTG CTGAACACTG TGGAGACACT CACGGCTGCT 
GGCACCCTCA TTGCCAAAGT CAAAGCCTTC CATTATGAGT GCAACAATGA GTCAGACAAG 
CAGGAGTTTG AGAAGGCCTT GGAGACCATT GCTGTCTCCT TTAGCAGCAC TGTATCCGAG 
TTCCTCATGG GTGAAGTGGA CAGCAACTGG GTTGTGGATG GGAGGAGGGG TGGGCTGGGG 
CAGGGGAGGG CTGGGCAGGG TGAGCCTGGG CTGAGGGGTG TTTACTCTGC AGGCTGCCTG 
TCCCCGGAGG ACGTGGACAC CCTGCTGCAG CGCTGTGAGG GGGGCGTGGA CGCTGCCCTG 
CAGTACGCCA AGAACATGGC CAAATACATG AAGGACCTCA TCGGCTACCT GGAGAAGCGG 
AGTGCGCTGG AGATGGACTT TGCCAAAGGC CTGCAGAAGA TCGTCCAGAA CTGCAGACAG 
AGTGTCATGC AGGAGCCGCA CATGCCCCTC CTGTCCATCT ACTCACTGGC GCTGGAACAG 
GACCTGGAGT TCGGCCATGG CCTGGTGCAG GCAGTGGGCA CGCTGCTGAC CCAGACCTTC 
ATGCAGCCCC TGAATCTGCG GCGGCTCGAA CATGAGAAGC GAAGGAAGGA GATCAAAGAG 
TCCTGGCACC GCGCCCAGAG GAAGCTGCAA GAGGCAGAGT CCAATCTCCG CAAGGCCAAG 
CAGGGCTACA TGCAGCGCTG CGAGGACCAT GACAAGGCCC GCTTCCTGGT GGCCAAGGCG 
GAGGAGGAGC AAGCCAACAT TGGGCCCGGT GCAGGGGGTG CAGCCTCCAA GACCCTGGAC 
AAGCGTCGTC GCCTGGAGGA GGAGGCCAAG AACAAGGCGG AGGAAGCAAT GGCCACCTAC 
CGTACATGCG TGGCCGATGC TAAGACACAG AAGCAGGAGC TGGAGGATAC CAAGGTGACG 
GCACTACGGC AGATCCAGGA GGTCATCCGG CAGAGTGACC AGACCATCAA GTCGGCCACC 
ATCTCATATT ACCAGATGAT GCACACGCAG ACGGCCCCAC TGCCCGTGCA CTTCCACATG 
CTGTATGAGA GCAGCAAGCT GTACGACCCA GGCCAGCAGT ACGCTTCCCA TGTGCGCCAG 
CTGCAGCGCG GCGAGGAGCC TGATGTGCAC TACGACTTCG AGCCCCACGT GTCCGCCAGT 
GCCTGGTCCC CAGTCCTGCG TGCTCGGAAA AGCAGCTTCA ATGTCAGCGA TGCTGTGGGG 
GCAGAGGCTG CCAGTAGCCC CCCGGAGGAC GGCGGGCCCA GCCAGGGAGA GATTGCCAAG 
GAGCGCAGGG GTGGGCGGGG CCACCAGGTG CACAAGTCGT GGCCCATCTC CATCTCCGAT 
TCGGAGGCCA GTCTGGACCC CAGCCCTGGC TCAGAGGACT TTAAGAAGTT TGAGCGTATG 
TCTTCCTGTG GCACCATGTC GTCCAACGAG GAGCTGGCCG ACCAGGAGGG CAGTGCAGGG 
GCATCAGCCT TTGATCAGGC TGACCTCAAT GGCATGACTC CAGAGCTGCC TGTGGCTGTG 
CCCAGCGGGC CCTTCCGCCA CGTGGGGCTG TCCAAGGCGG CCCGGACCCA CCGGCTTCGG 
AAGCTCCGCA CACCGGCCAA GTGCCGGGAA TGCAACAGCT ACGTGTACTT CCAGGGCGCC 
GAGTGTGAGG AGTGCTGCCT GGCCTGCCAC AAGAAGTGCC TGGAGACCCT GGCCATCCAG 
TGTGGCCACA AGAAGCTGCA GGGCCGTCTG CAGCTCTTTG GCCAGGACTT CAGCCGTGCT 
GCCTGCAGCA CCCCCGACGG CGTGCCCTTC ATCATCAAGA AGTGCATCTT TGAGATCGAG 
CAGCGGGCGC TGCGCACCAA GGGCATCTAC CGGGTCAATG GGGTGAAGAC ACGTGTAGAG 
AAGCTGTGCC AGGCCTTCGA GAATGGCAAG GAGCTGGTGG AATTGTCGCA AGCCTCACCC 
CACGACATCA GCAATGTCCT CAAGCTCTAT CTGCGCCAGC TCCCTGAGCC GCTCATTTCC 
TTCCGCTTCT ACCATGAGCT TGTGGGGATG GCCAAAGACA GTCTGAAGGC CGAGGCCGAG 
GCCAAGGCAG CATCTCGGGG TCGGCCGGAT GCCACAGAGA GGGAGGCTGC AGCCATGGCC 
ATGGCGAGCC GGTTGCGGGA ACTCCTACGG GACCTGCCTC GGGATAACTG GGCCACACTG 
CGGTACCTGA TGCGGCACCT GCGCAGGATC GTGGAGGTGG AACAGGACAA CAAGATGACA 
CCAGGGAACC TGGGCATTGT GTTTGGGCCC ACGCTGCTGC GGCCCCGGCC CACTGAGGCC 
ACGGTGTCCC TTTCCTCCCT GGTGGACTAC CCCCACCAGG CCTGCATCGT GGAGACCCTC 
ATCACCCACT TCAGCCTGGT CTTCAAGGAG GAGCCCGAAG AGGCCCCAGG GGGCCAGGAT 
GGGGTATCCA GCCAGCGACC CGAGGTGGTG GTCCAGGCAC CGTACCTGGG GGGCAGCGGG 
GGTGCAGCCT TTCCCTTGAC AGAGGAGGCT GAGGATGGGG TCCTTGAACC CCATGTCGCC 
TCCAATGACT CCGACTCAGA GCTGGAGGAG GCCTCAGACC TGCAGTCCCC GGTGGGTGGG 
GCTGCACTGC ACCGGCTCAG TTTTCTGGAG AGGCAGGGCG GCGAGGCAGG CACAGAGGGC 
AGTCAGGGCA GCCGCAGCGG CAGCGAAGAG CAGCTGGGTG CCACAGCCGG CGAGTACGGG 
CCTGGGGCCT GGGAGGGCCC TGGGGAGGAG CCAGCCCGGA GGCTCTCCGA GTACAACACC 
AACCAGTGCA ACAACACCAG CCAGTCTTGG CTGCCAGCTG TGAGGCTGCG TGGTGGGCGG 
CTGGTAGGGG GCAACAGCTG CAAGAGGCAG CCAGAGTTTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ARHGAP45 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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