PUF60 cDNA ORF clone, Bos taurus(cattle)

The following PUF60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa00482 NM_001046133.2
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25744 NM_001359972.1
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25746 NM_001359973.1
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25748 NM_001359974.1
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25744 XM_005215169.3
Latest version!
Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25745 XM_005215170.4
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25746 XM_005215171.3
Latest version!
Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25747 XM_005215172.4
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa25748 XM_005215173.3
Latest version!
Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa48446 XM_010811834.3
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa239074 NM_001046133.3
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa222251 XM_025001511.1
Latest version!
Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa25745 XM_005215170.3 Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa48446 XM_010811834.2 Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa25747 XM_005215172.3 Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa00482
    Clone ID Related Accession (Same CDS sequence) NM_001046133.2
    Accession Version NM_001046133.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105447.1. On Aug 31, 2012 this sequence version replaced NM_001046133.1. ##Evidence-Data-START## Transcript exon combination :: BC105447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00120237, SAMN00120238 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCACTGGC CATCATGTGC
    CGGGTCTACG TGGGCTCCAT CTACTACGAG CTAGGGGAGG ACACCATCCG CCAGGCGTTC
    GCCCCCTTTG GGCCTATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CGTTTGTGGA GTATGAGGTC CCAGAAGCCG CCCAGCTGGC CTTGGAGCAG
    ATGAATTCCG TGATGCTGGG AGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCTCAGC CCATCATAGA CCAGCTGGCC GAGGAGGCGC GAGCCTTCAA CCGCATCTAC
    GTGGCTTCCG TGCACCAGGA CCTCTCGGAT GATGACATCA AGAGTGTGTT TGAGGCCTTT
    GGCAAGATCA AATCCTGCAC GCTGGCTCGG GACCCCACGA CTGGCAAACA CAAGGGCTAT
    GGCTTCATTG AATATGAGAA AGCCCAATCG TCCCAGGATG CTGTGTCTTC CATGAACCTT
    TTTGACCTGG GGGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCCCC TATGCCCCTG
    CTTACACCGG CCACACCTGG AGGCCTCCCG CCGGCTGCTG CCGTGGCCGC AGCTGCAGCC
    ACAGCCAAGA TTACAGCTCA GGAAGCAGTG GCTGGAGCAG CAGTACTGGG TACCCTAGCC
    ACTCCTGGAC TGGTGTCCCC TGCACTGACT CTGGCCCAGC CCCTGGGGGC TCTACCCCAG
    GCCGTCATGG CTGCCCAGGC CCCAGGAGTC ATCACAGGTG TGACACCGGC TCGGCCTCCC
    ATTCCGGTCA CCATCCCCTC TGTTGGTGTG GTGAATCCCA TCCTGGCCAG CCCTCCGACA
    CTTGGTCTCC TGGAGCCTAA GAAGGAGAAG GAGGAGGAGG AGCTGTTTCC CGAGTCGGAG
    CGGCCGGAGA TGCTGAGTGA GCAGGAGCAC ATGAGCATCT CAGGAAGCAG CGCCCGCCAC
    ATGGTGATGC AGAAGCTCCT CCGCAAGCAG GAGTCCACAG TGATGGTTCT GCGTAACATG
    GTGGACCCCA AGGACATCGA CGATGACCTT GAAGGGGAGG TGACTGAGGA GTGTGGCAAG
    TTTGGTGCTG TCAACCGTGT CATCATCTAC CAAGAGAAGC AGGGCGAGGA AGAGGACGCG
    GAGATCATTG TCAAGATTTT TGTGGAGTTT TCCGTAGCCT CTGAGACTCA CAAGGCCATC
    CAGGACCTCA ATGGGCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTATGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039598.1
    CDS13..1605
    Translation

    Target ORF information:

    RefSeq Version NM_001046133.2
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001046133.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCACTGGC CATCATGTGC
    CGGGTCTACG TGGGCTCCAT CTACTACGAG CTAGGGGAGG ACACCATCCG CCAGGCGTTC
    GCCCCCTTTG GGCCTATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CGTTTGTGGA GTATGAGGTC CCAGAAGCCG CCCAGCTGGC CTTGGAGCAG
    ATGAATTCCG TGATGCTGGG AGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCTCAGC CCATCATAGA CCAGCTGGCC GAGGAGGCGC GAGCCTTCAA CCGCATCTAC
    GTGGCTTCCG TGCACCAGGA CCTCTCGGAT GATGACATCA AGAGTGTGTT TGAGGCCTTT
    GGCAAGATCA AATCCTGCAC GCTGGCTCGG GACCCCACGA CTGGCAAACA CAAGGGCTAT
    GGCTTCATTG AATATGAGAA AGCCCAATCG TCCCAGGATG CTGTGTCTTC CATGAACCTT
    TTTGACCTGG GGGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCCCC TATGCCCCTG
    CTTACACCGG CCACACCTGG AGGCCTCCCG CCGGCTGCTG CCGTGGCCGC AGCTGCAGCC
    ACAGCCAAGA TTACAGCTCA GGAAGCAGTG GCTGGAGCAG CAGTACTGGG TACCCTAGCC
    ACTCCTGGAC TGGTGTCCCC TGCACTGACT CTGGCCCAGC CCCTGGGGGC TCTACCCCAG
    GCCGTCATGG CTGCCCAGGC CCCAGGAGTC ATCACAGGTG TGACACCGGC TCGGCCTCCC
    ATTCCGGTCA CCATCCCCTC TGTTGGTGTG GTGAATCCCA TCCTGGCCAG CCCTCCGACA
    CTTGGTCTCC TGGAGCCTAA GAAGGAGAAG GAGGAGGAGG AGCTGTTTCC CGAGTCGGAG
    CGGCCGGAGA TGCTGAGTGA GCAGGAGCAC ATGAGCATCT CAGGAAGCAG CGCCCGCCAC
    ATGGTGATGC AGAAGCTCCT CCGCAAGCAG GAGTCCACAG TGATGGTTCT GCGTAACATG
    GTGGACCCCA AGGACATCGA CGATGACCTT GAAGGGGAGG TGACTGAGGA GTGTGGCAAG
    TTTGGTGCTG TCAACCGTGT CATCATCTAC CAAGAGAAGC AGGGCGAGGA AGAGGACGCG
    GAGATCATTG TCAAGATTTT TGTGGAGTTT TCCGTAGCCT CTGAGACTCA CAAGGCCATC
    CAGGACCTCA ATGGGCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTATGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25744
    Clone ID Related Accession (Same CDS sequence) NM_001359972.1 , XM_005215169.3
    Accession Version NM_001359972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE251077.1, BC105447.1, DV852174.1 and NKLS02000014.1. On Jan 24, 2018 this sequence version replaced XM_005215169.3. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.25930.1, SRR5571256.3979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CAGCAGTGAC AATGGGCTTT GGAGATCCTC TCTCACCTTT GCAATCGATG
    GCGGCTCAGC GGCAGCGGGC ACTGGCCATC ATGTGCCGGG TCTACGTGGG CTCCATCTAC
    TACGAGCTAG GGGAGGACAC CATCCGCCAG GCGTTCGCCC CCTTTGGGCC TATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCGTT TGTGGAGTAT
    GAGGTCCCAG AAGCCGCCCA GCTGGCCTTG GAGCAGATGA ATTCCGTGAT GCTGGGAGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CTCAGCCCAT CATAGACCAG
    CTGGCCGAGG AGGCGCGAGC CTTCAACCGC ATCTACGTGG CTTCCGTGCA CCAGGACCTC
    TCGGATGATG ACATCAAGAG TGTGTTTGAG GCCTTTGGCA AGATCAAATC CTGCACGCTG
    GCTCGGGACC CCACGACTGG CAAACACAAG GGCTATGGCT TCATTGAATA TGAGAAAGCC
    CAATCGTCCC AGGATGCTGT GTCTTCCATG AACCTTTTTG ACCTGGGGGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCCCCTATG CCCCTGCTTA CACCGGCCAC ACCTGGAGGC
    CTCCCGCCGG CTGCTGCCGT GGCCGCAGCT GCAGCCACAG CCAAGATTAC AGCTCAGGAA
    GCAGTGGCTG GAGCAGCAGT ACTGGGTACC CTAGCCACTC CTGGACTGGT GTCCCCTGCA
    CTGACTCTGG CCCAGCCCCT GGGGGCTCTA CCCCAGGCCG TCATGGCTGC CCAGGCCCCA
    GGAGTCATCA CAGGTGTGAC ACCGGCTCGG CCTCCCATTC CGGTCACCAT CCCCTCTGTT
    GGTGTGGTGA ATCCCATCCT GGCCAGCCCT CCGACACTTG GTCTCCTGGA GCCTAAGAAG
    GAGAAGGAGG AGGAGGAGCT GTTTCCCGAG TCGGAGCGGC CGGAGATGCT GAGTGAGCAG
    GAGCACATGA GCATCTCAGG AAGCAGCGCC CGCCACATGG TGATGCAGAA GCTCCTCCGC
    AAGCAGGAGT CCACAGTGAT GGTTCTGCGT AACATGGTGG ACCCCAAGGA CATCGACGAT
    GACCTGGAAG GGGAGGTGAC TGAGGAGTGT GGCAAGTTTG GTGCTGTCAA CCGTGTCATC
    ATCTACCAAG AGAAGCAGGG CGAGGAAGAG GACGCGGAGA TCATTGTCAA GATTTTTGTG
    GAGTTTTCCG TAGCCTCTGA GACTCACAAG GCCATCCAGG CCCTCAATGG GCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAT GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346901.1
    CDS206..1894
    Translation

    Target ORF information:

    RefSeq Version NM_001359972.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001359972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CAGCAGTGAC AATGGGCTTT GGAGATCCTC TCTCACCTTT GCAATCGATG
    GCGGCTCAGC GGCAGCGGGC ACTGGCCATC ATGTGCCGGG TCTACGTGGG CTCCATCTAC
    TACGAGCTAG GGGAGGACAC CATCCGCCAG GCGTTCGCCC CCTTTGGGCC TATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCGTT TGTGGAGTAT
    GAGGTCCCAG AAGCCGCCCA GCTGGCCTTG GAGCAGATGA ATTCCGTGAT GCTGGGAGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CTCAGCCCAT CATAGACCAG
    CTGGCCGAGG AGGCGCGAGC CTTCAACCGC ATCTACGTGG CTTCCGTGCA CCAGGACCTC
    TCGGATGATG ACATCAAGAG TGTGTTTGAG GCCTTTGGCA AGATCAAATC CTGCACGCTG
    GCTCGGGACC CCACGACTGG CAAACACAAG GGCTATGGCT TCATTGAATA TGAGAAAGCC
    CAATCGTCCC AGGATGCTGT GTCTTCCATG AACCTTTTTG ACCTGGGGGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCCCCTATG CCCCTGCTTA CACCGGCCAC ACCTGGAGGC
    CTCCCGCCGG CTGCTGCCGT GGCCGCAGCT GCAGCCACAG CCAAGATTAC AGCTCAGGAA
    GCAGTGGCTG GAGCAGCAGT ACTGGGTACC CTAGCCACTC CTGGACTGGT GTCCCCTGCA
    CTGACTCTGG CCCAGCCCCT GGGGGCTCTA CCCCAGGCCG TCATGGCTGC CCAGGCCCCA
    GGAGTCATCA CAGGTGTGAC ACCGGCTCGG CCTCCCATTC CGGTCACCAT CCCCTCTGTT
    GGTGTGGTGA ATCCCATCCT GGCCAGCCCT CCGACACTTG GTCTCCTGGA GCCTAAGAAG
    GAGAAGGAGG AGGAGGAGCT GTTTCCCGAG TCGGAGCGGC CGGAGATGCT GAGTGAGCAG
    GAGCACATGA GCATCTCAGG AAGCAGCGCC CGCCACATGG TGATGCAGAA GCTCCTCCGC
    AAGCAGGAGT CCACAGTGAT GGTTCTGCGT AACATGGTGG ACCCCAAGGA CATCGACGAT
    GACCTGGAAG GGGAGGTGAC TGAGGAGTGT GGCAAGTTTG GTGCTGTCAA CCGTGTCATC
    ATCTACCAAG AGAAGCAGGG CGAGGAAGAG GACGCGGAGA TCATTGTCAA GATTTTTGTG
    GAGTTTTCCG TAGCCTCTGA GACTCACAAG GCCATCCAGG CCCTCAATGG GCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAT GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25746
    Clone ID Related Accession (Same CDS sequence) NM_001359973.1 , XM_005215171.3
    Accession Version NM_001359973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE251077.1, EH373261.1, BC105447.1, DV852174.1 and NKLS02000014.1. On Jan 24, 2018 this sequence version replaced XM_005215171.3. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.31671.1, SRR5571260.18416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CGGCTCAGCG GCAGCGGGCA CTGGCCATCA TGTGCCGGGT CTACGTGGGC
    TCCATCTACT ACGAGCTAGG GGAGGACACC ATCCGCCAGG CGTTCGCCCC CTTTGGGCCT
    ATCAAGAGCA TCGACATGTC CTGGGACTCC GTCACCATGA AGCACAAGGG CTTTGCGTTT
    GTGGAGTATG AGGTCCCAGA AGCCGCCCAG CTGGCCTTGG AGCAGATGAA TTCCGTGATG
    CTGGGAGGCA GGAACATCAA GGTGGGCAGA CCCAGCAACA TAGGGCAGGC TCAGCCCATC
    ATAGACCAGC TGGCCGAGGA GGCGCGAGCC TTCAACCGCA TCTACGTGGC TTCCGTGCAC
    CAGGACCTCT CGGATGATGA CATCAAGAGT GTGTTTGAGG CCTTTGGCAA GATCAAATCC
    TGCACGCTGG CTCGGGACCC CACGACTGGC AAACACAAGG GCTATGGCTT CATTGAATAT
    GAGAAAGCCC AATCGTCCCA GGATGCTGTG TCTTCCATGA ACCTTTTTGA CCTGGGGGGC
    CAGTACTTGC GGGTGGGCAA GGCTGTCACA CCCCCTATGC CCCTGCTTAC ACCGGCCACA
    CCTGGAGGCC TCCCGCCGGC TGCTGCCGTG GCCGCAGCTG CAGCCACAGC CAAGATTACA
    GCTCAGGAAG CAGTGGCTGG AGCAGCAGTA CTGGGTACCC TAGCCACTCC TGGACTGGTG
    TCCCCTGCAC TGACTCTGGC CCAGCCCCTG GGGGCTCTAC CCCAGGCCGT CATGGCTGCC
    CAGGCCCCAG GAGTCATCAC AGGTGTGACA CCGGCTCGGC CTCCCATTCC GGTCACCATC
    CCCTCTGTTG GTGTGGTGAA TCCCATCCTG GCCAGCCCTC CGACACTTGG TCTCCTGGAG
    CCTAAGAAGG AGAAGGAGGA GGAGGAGCTG TTTCCCGAGT CGGAGCGGCC GGAGATGCTG
    AGTGAGCAGG AGCACATGAG CATCTCAGGA AGCAGCGCCC GCCACATGGT GATGCAGAAG
    CTCCTCCGCA AGCAGGAGTC CACAGTGATG GTTCTGCGTA ACATGGTGGA CCCCAAGGAC
    ATCGACGATG ACCTGGAAGG GGAGGTGACT GAGGAGTGTG GCAAGTTTGG TGCTGTCAAC
    CGTGTCATCA TCTACCAAGA GAAGCAGGGC GAGGAAGAGG ACGCGGAGAT CATTGTCAAG
    ATTTTTGTGG AGTTTTCCGT AGCCTCTGAG ACTCACAAGG CCATCCAGGC CCTCAATGGG
    CGCTGGTTTG CTGGCCGCAA GGTGGTGGCT GAAGTGTATG ACCAGGAGCG TTTTGATAAC
    AGTGACCTCT CTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346902.1
    CDS206..1843
    Translation

    Target ORF information:

    RefSeq Version NM_001359973.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001359973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CGGCTCAGCG GCAGCGGGCA CTGGCCATCA TGTGCCGGGT CTACGTGGGC
    TCCATCTACT ACGAGCTAGG GGAGGACACC ATCCGCCAGG CGTTCGCCCC CTTTGGGCCT
    ATCAAGAGCA TCGACATGTC CTGGGACTCC GTCACCATGA AGCACAAGGG CTTTGCGTTT
    GTGGAGTATG AGGTCCCAGA AGCCGCCCAG CTGGCCTTGG AGCAGATGAA TTCCGTGATG
    CTGGGAGGCA GGAACATCAA GGTGGGCAGA CCCAGCAACA TAGGGCAGGC TCAGCCCATC
    ATAGACCAGC TGGCCGAGGA GGCGCGAGCC TTCAACCGCA TCTACGTGGC TTCCGTGCAC
    CAGGACCTCT CGGATGATGA CATCAAGAGT GTGTTTGAGG CCTTTGGCAA GATCAAATCC
    TGCACGCTGG CTCGGGACCC CACGACTGGC AAACACAAGG GCTATGGCTT CATTGAATAT
    GAGAAAGCCC AATCGTCCCA GGATGCTGTG TCTTCCATGA ACCTTTTTGA CCTGGGGGGC
    CAGTACTTGC GGGTGGGCAA GGCTGTCACA CCCCCTATGC CCCTGCTTAC ACCGGCCACA
    CCTGGAGGCC TCCCGCCGGC TGCTGCCGTG GCCGCAGCTG CAGCCACAGC CAAGATTACA
    GCTCAGGAAG CAGTGGCTGG AGCAGCAGTA CTGGGTACCC TAGCCACTCC TGGACTGGTG
    TCCCCTGCAC TGACTCTGGC CCAGCCCCTG GGGGCTCTAC CCCAGGCCGT CATGGCTGCC
    CAGGCCCCAG GAGTCATCAC AGGTGTGACA CCGGCTCGGC CTCCCATTCC GGTCACCATC
    CCCTCTGTTG GTGTGGTGAA TCCCATCCTG GCCAGCCCTC CGACACTTGG TCTCCTGGAG
    CCTAAGAAGG AGAAGGAGGA GGAGGAGCTG TTTCCCGAGT CGGAGCGGCC GGAGATGCTG
    AGTGAGCAGG AGCACATGAG CATCTCAGGA AGCAGCGCCC GCCACATGGT GATGCAGAAG
    CTCCTCCGCA AGCAGGAGTC CACAGTGATG GTTCTGCGTA ACATGGTGGA CCCCAAGGAC
    ATCGACGATG ACCTGGAAGG GGAGGTGACT GAGGAGTGTG GCAAGTTTGG TGCTGTCAAC
    CGTGTCATCA TCTACCAAGA GAAGCAGGGC GAGGAAGAGG ACGCGGAGAT CATTGTCAAG
    ATTTTTGTGG AGTTTTCCGT AGCCTCTGAG ACTCACAAGG CCATCCAGGC CCTCAATGGG
    CGCTGGTTTG CTGGCCGCAA GGTGGTGGCT GAAGTGTATG ACCAGGAGCG TTTTGATAAC
    AGTGACCTCT CTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25748
    Clone ID Related Accession (Same CDS sequence) NM_001359974.1 , XM_005215173.3
    Accession Version NM_001359974.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE251077.1, EE247999.1, BC105447.1, DV852174.1 and NKLS02000014.1. On Jan 24, 2018 this sequence version replaced XM_005215173.3. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.3350.1, SRR5571259.11098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCACTGGCC
    ATCATGTGCC GGGTCTACGT GGGCTCCATC TACTACGAGC TAGGGGAGGA CACCATCCGC
    CAGGCGTTCG CCCCCTTTGG GCCTATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC GTTTGTGGAG TATGAGGTCC CAGAAGCCGC CCAGCTGGCC
    TTGGAGCAGA TGAATTCCGT GATGCTGGGA GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCTCAGCC CATCATAGAC CAGCTGGCCG AGGAGGCGCG AGCCTTCAAC
    CGCATCTACG TGGCTTCCGT GCACCAGGAC CTCTCGGATG ATGACATCAA GAGTGTGTTT
    GAGGCCTTTG GCAAGATCAA ATCCTGCACG CTGGCTCGGG ACCCCACGAC TGGCAAACAC
    AAGGGCTATG GCTTCATTGA ATATGAGAAA GCCCAATCGT CCCAGGATGC TGTGTCTTCC
    ATGAACCTTT TTGACCTGGG GGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCCCCT
    ATGCCCCTGC TTACACCGGC CACACCTGGA GGCCTCCCGC CGGCTGCTGC CGTGGCCGCA
    GCTGCAGCCA CAGCCAAGAT TACAGCTCAG GAAGCAGTGG CTGGAGCAGC AGTACTGGGT
    ACCCTAGCCA CTCCTGGACT GGTGTCCCCT GCACTGACTC TGGCCCAGCC CCTGGGGGCT
    CTACCCCAGG CCGTCATGGC TGCCCAGGCC CCAGGAGTCA TCACAGGTGT GACACCGGCT
    CGGCCTCCCA TTCCGGTCAC CATCCCCTCT GTTGGTGTGG TGAATCCCAT CCTGGCCAGC
    CCTCCGACAC TTGGTCTCCT GGAGCCTAAG AAGGAGAAGG AGGAGGAGGA GCTGTTTCCC
    GAGTCGGAGC GGCCGGAGAT GCTGAGTGAG CAGGAGCACA TGAGCATCTC AGGAAGCAGC
    GCCCGCCACA TGGTGATGCA GAAGCTCCTC CGCAAGCAGG AGTCCACAGT GATGGTTCTG
    CGTAACATGG TGGACCCCAA GGACATCGAC GATGACCTGG AAGGGGAGGT GACTGAGGAG
    TGTGGCAAGT TTGGTGCTGT CAACCGTGTC ATCATCTACC AAGAGAAGCA GGGCGAGGAA
    GAGGACGCGG AGATCATTGT CAAGATTTTT GTGGAGTTTT CCGTAGCCTC TGAGACTCAC
    AAGGCCATCC AGGCCCTCAA TGGGCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TATGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346903.1
    CDS206..1747
    Translation

    Target ORF information:

    RefSeq Version NM_001359974.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001359974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCACTGGCC
    ATCATGTGCC GGGTCTACGT GGGCTCCATC TACTACGAGC TAGGGGAGGA CACCATCCGC
    CAGGCGTTCG CCCCCTTTGG GCCTATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC GTTTGTGGAG TATGAGGTCC CAGAAGCCGC CCAGCTGGCC
    TTGGAGCAGA TGAATTCCGT GATGCTGGGA GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCTCAGCC CATCATAGAC CAGCTGGCCG AGGAGGCGCG AGCCTTCAAC
    CGCATCTACG TGGCTTCCGT GCACCAGGAC CTCTCGGATG ATGACATCAA GAGTGTGTTT
    GAGGCCTTTG GCAAGATCAA ATCCTGCACG CTGGCTCGGG ACCCCACGAC TGGCAAACAC
    AAGGGCTATG GCTTCATTGA ATATGAGAAA GCCCAATCGT CCCAGGATGC TGTGTCTTCC
    ATGAACCTTT TTGACCTGGG GGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCCCCT
    ATGCCCCTGC TTACACCGGC CACACCTGGA GGCCTCCCGC CGGCTGCTGC CGTGGCCGCA
    GCTGCAGCCA CAGCCAAGAT TACAGCTCAG GAAGCAGTGG CTGGAGCAGC AGTACTGGGT
    ACCCTAGCCA CTCCTGGACT GGTGTCCCCT GCACTGACTC TGGCCCAGCC CCTGGGGGCT
    CTACCCCAGG CCGTCATGGC TGCCCAGGCC CCAGGAGTCA TCACAGGTGT GACACCGGCT
    CGGCCTCCCA TTCCGGTCAC CATCCCCTCT GTTGGTGTGG TGAATCCCAT CCTGGCCAGC
    CCTCCGACAC TTGGTCTCCT GGAGCCTAAG AAGGAGAAGG AGGAGGAGGA GCTGTTTCCC
    GAGTCGGAGC GGCCGGAGAT GCTGAGTGAG CAGGAGCACA TGAGCATCTC AGGAAGCAGC
    GCCCGCCACA TGGTGATGCA GAAGCTCCTC CGCAAGCAGG AGTCCACAGT GATGGTTCTG
    CGTAACATGG TGGACCCCAA GGACATCGAC GATGACCTGG AAGGGGAGGT GACTGAGGAG
    TGTGGCAAGT TTGGTGCTGT CAACCGTGTC ATCATCTACC AAGAGAAGCA GGGCGAGGAA
    GAGGACGCGG AGATCATTGT CAAGATTTTT GTGGAGTTTT CCGTAGCCTC TGAGACTCAC
    AAGGCCATCC AGGCCCTCAA TGGGCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TATGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25744
    Clone ID Related Accession (Same CDS sequence) NM_001359972.1 , XM_005215169.3
    Accession Version XM_005215169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CAGCAGTGAC AATGGGCTTT GGAGATCCTC TCTCACCTTT GCAATCGATG
    GCGGCTCAGC GGCAGCGGGC ACTGGCCATC ATGTGCCGGG TCTACGTGGG CTCCATCTAC
    TACGAGCTAG GGGAGGACAC CATCCGCCAG GCGTTCGCCC CCTTTGGGCC TATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCGTT TGTGGAGTAT
    GAGGTCCCAG AAGCCGCCCA GCTGGCCTTG GAGCAGATGA ATTCCGTGAT GCTGGGAGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CTCAGCCCAT CATAGACCAG
    CTGGCCGAGG AGGCGCGAGC CTTCAACCGC ATCTACGTGG CTTCCGTGCA CCAGGACCTC
    TCGGATGATG ACATCAAGAG TGTGTTTGAG GCCTTTGGCA AGATCAAATC CTGCACGCTG
    GCTCGGGACC CCACGACTGG CAAACACAAG GGCTATGGCT TCATTGAATA TGAGAAAGCC
    CAATCGTCCC AGGATGCTGT GTCTTCCATG AACCTTTTTG ACCTGGGGGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCCCCTATG CCCCTGCTTA CACCGGCCAC ACCTGGAGGC
    CTCCCGCCGG CTGCTGCCGT GGCCGCAGCT GCAGCCACAG CCAAGATTAC AGCTCAGGAA
    GCAGTGGCTG GAGCAGCAGT ACTGGGTACC CTAGCCACTC CTGGACTGGT GTCCCCTGCA
    CTGACTCTGG CCCAGCCCCT GGGGGCTCTA CCCCAGGCCG TCATGGCTGC CCAGGCCCCA
    GGAGTCATCA CAGGTGTGAC ACCGGCTCGG CCTCCCATTC CGGTCACCAT CCCCTCTGTT
    GGTGTGGTGA ATCCCATCCT GGCCAGCCCT CCGACACTTG GTCTCCTGGA GCCTAAGAAG
    GAGAAGGAGG AGGAGGAGCT GTTTCCCGAG TCGGAGCGGC CGGAGATGCT GAGTGAGCAG
    GAGCACATGA GCATCTCAGG AAGCAGCGCC CGCCACATGG TGATGCAGAA GCTCCTCCGC
    AAGCAGGAGT CCACAGTGAT GGTTCTGCGT AACATGGTGG ACCCCAAGGA CATCGACGAT
    GACCTGGAAG GGGAGGTGAC TGAGGAGTGT GGCAAGTTTG GTGCTGTCAA CCGTGTCATC
    ATCTACCAAG AGAAGCAGGG CGAGGAAGAG GACGCGGAGA TCATTGTCAA GATTTTTGTG
    GAGTTTTCCG TAGCCTCTGA GACTCACAAG GCCATCCAGG CCCTCAATGG GCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAT GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215226.1
    CDS201..1889
    Translation

    Target ORF information:

    RefSeq Version XM_005215169.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CAGCAGTGAC AATGGGCTTT GGAGATCCTC TCTCACCTTT GCAATCGATG
    GCGGCTCAGC GGCAGCGGGC ACTGGCCATC ATGTGCCGGG TCTACGTGGG CTCCATCTAC
    TACGAGCTAG GGGAGGACAC CATCCGCCAG GCGTTCGCCC CCTTTGGGCC TATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCGTT TGTGGAGTAT
    GAGGTCCCAG AAGCCGCCCA GCTGGCCTTG GAGCAGATGA ATTCCGTGAT GCTGGGAGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CTCAGCCCAT CATAGACCAG
    CTGGCCGAGG AGGCGCGAGC CTTCAACCGC ATCTACGTGG CTTCCGTGCA CCAGGACCTC
    TCGGATGATG ACATCAAGAG TGTGTTTGAG GCCTTTGGCA AGATCAAATC CTGCACGCTG
    GCTCGGGACC CCACGACTGG CAAACACAAG GGCTATGGCT TCATTGAATA TGAGAAAGCC
    CAATCGTCCC AGGATGCTGT GTCTTCCATG AACCTTTTTG ACCTGGGGGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCCCCTATG CCCCTGCTTA CACCGGCCAC ACCTGGAGGC
    CTCCCGCCGG CTGCTGCCGT GGCCGCAGCT GCAGCCACAG CCAAGATTAC AGCTCAGGAA
    GCAGTGGCTG GAGCAGCAGT ACTGGGTACC CTAGCCACTC CTGGACTGGT GTCCCCTGCA
    CTGACTCTGG CCCAGCCCCT GGGGGCTCTA CCCCAGGCCG TCATGGCTGC CCAGGCCCCA
    GGAGTCATCA CAGGTGTGAC ACCGGCTCGG CCTCCCATTC CGGTCACCAT CCCCTCTGTT
    GGTGTGGTGA ATCCCATCCT GGCCAGCCCT CCGACACTTG GTCTCCTGGA GCCTAAGAAG
    GAGAAGGAGG AGGAGGAGCT GTTTCCCGAG TCGGAGCGGC CGGAGATGCT GAGTGAGCAG
    GAGCACATGA GCATCTCAGG AAGCAGCGCC CGCCACATGG TGATGCAGAA GCTCCTCCGC
    AAGCAGGAGT CCACAGTGAT GGTTCTGCGT AACATGGTGG ACCCCAAGGA CATCGACGAT
    GACCTGGAAG GGGAGGTGAC TGAGGAGTGT GGCAAGTTTG GTGCTGTCAA CCGTGTCATC
    ATCTACCAAG AGAAGCAGGG CGAGGAAGAG GACGCGGAGA TCATTGTCAA GATTTTTGTG
    GAGTTTTCCG TAGCCTCTGA GACTCACAAG GCCATCCAGG CCCTCAATGG GCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAT GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25745
    Clone ID Related Accession (Same CDS sequence) XM_005215170.4 , XM_005215170.3
    Accession Version XM_005215170.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215170.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCAG CAGTGACAAT GGGCTTTGGA GATCCTCTCT CACCTTTGCA ATCGATGGCG
    GCTCAGCGGC AGCGGGCACT GGCCATCATG TGCCGGGTCT ACGTGGGCTC CATCTACTAC
    GAGCTAGGGG AGGACACCAT CCGCCAGGCG TTCGCCCCCT TTGGGCCTAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCGTTTGT GGAGTATGAG
    GTCCCAGAAG CCGCCCAGCT GGCCTTGGAG CAGATGAATT CCGTGATGCT GGGAGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCTC AGCCCATCAT AGACCAGCTG
    GCCGAGGAGG CGCGAGCCTT CAACCGCATC TACGTGGCTT CCGTGCACCA GGACCTCTCG
    GATGATGACA TCAAGAGTGT GTTTGAGGCC TTTGGCAAGA TCAAATCCTG CACGCTGGCT
    CGGGACCCCA CGACTGGCAA ACACAAGGGC TATGGCTTCA TTGAATATGA GAAAGCCCAA
    TCGTCCCAGG ATGCTGTGTC TTCCATGAAC CTTTTTGACC TGGGGGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC CCCTATGCCC CTGCTTACAC CGGCCACACC TGGAGGCCTC
    CCGCCGGCTG CTGCCGTGGC CGCAGCTGCA GCCACAGCCA AGATTACAGC TCAGGAAGCA
    GTGGCTGGAG CAGCAGTACT GGGTACCCTA GCCACTCCTG GACTGGTGTC CCCTGCACTG
    ACTCTGGCCC AGCCCCTGGG GGCTCTACCC CAGGCCGTCA TGGCTGCCCA GGCCCCAGGA
    GTCATCACAG GTGTGACACC GGCTCGGCCT CCCATTCCGG TCACCATCCC CTCTGTTGGT
    GTGGTGAATC CCATCCTGGC CAGCCCTCCG ACACTTGGTC TCCTGGAGCC TAAGAAGGAG
    AAGGAGGAGG AGGAGCTGTT TCCCGAGTCG GAGCGGCCGG AGATGCTGAG TGAGCAGGAG
    CACATGAGCA TCTCAGGAAG CAGCGCCCGC CACATGGTGA TGCAGAAGCT CCTCCGCAAG
    CAGGAGTCCA CAGTGATGGT TCTGCGTAAC ATGGTGGACC CCAAGGACAT CGACGATGAC
    CTGGAAGGGG AGGTGACTGA GGAGTGTGGC AAGTTTGGTG CTGTCAACCG TGTCATCATC
    TACCAAGAGA AGCAGGGCGA GGAAGAGGAC GCGGAGATCA TTGTCAAGAT TTTTGTGGAG
    TTTTCCGTAG CCTCTGAGAC TCACAAGGCC ATCCAGGCCC TCAATGGGCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTATGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215227.1
    CDS145..1830
    Translation

    Target ORF information:

    RefSeq Version XM_005215170.4
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215170.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCAG CAGTGACAAT GGGCTTTGGA GATCCTCTCT CACCTTTGCA ATCGATGGCG
    GCTCAGCGGC AGCGGGCACT GGCCATCATG TGCCGGGTCT ACGTGGGCTC CATCTACTAC
    GAGCTAGGGG AGGACACCAT CCGCCAGGCG TTCGCCCCCT TTGGGCCTAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCGTTTGT GGAGTATGAG
    GTCCCAGAAG CCGCCCAGCT GGCCTTGGAG CAGATGAATT CCGTGATGCT GGGAGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCTC AGCCCATCAT AGACCAGCTG
    GCCGAGGAGG CGCGAGCCTT CAACCGCATC TACGTGGCTT CCGTGCACCA GGACCTCTCG
    GATGATGACA TCAAGAGTGT GTTTGAGGCC TTTGGCAAGA TCAAATCCTG CACGCTGGCT
    CGGGACCCCA CGACTGGCAA ACACAAGGGC TATGGCTTCA TTGAATATGA GAAAGCCCAA
    TCGTCCCAGG ATGCTGTGTC TTCCATGAAC CTTTTTGACC TGGGGGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC CCCTATGCCC CTGCTTACAC CGGCCACACC TGGAGGCCTC
    CCGCCGGCTG CTGCCGTGGC CGCAGCTGCA GCCACAGCCA AGATTACAGC TCAGGAAGCA
    GTGGCTGGAG CAGCAGTACT GGGTACCCTA GCCACTCCTG GACTGGTGTC CCCTGCACTG
    ACTCTGGCCC AGCCCCTGGG GGCTCTACCC CAGGCCGTCA TGGCTGCCCA GGCCCCAGGA
    GTCATCACAG GTGTGACACC GGCTCGGCCT CCCATTCCGG TCACCATCCC CTCTGTTGGT
    GTGGTGAATC CCATCCTGGC CAGCCCTCCG ACACTTGGTC TCCTGGAGCC TAAGAAGGAG
    AAGGAGGAGG AGGAGCTGTT TCCCGAGTCG GAGCGGCCGG AGATGCTGAG TGAGCAGGAG
    CACATGAGCA TCTCAGGAAG CAGCGCCCGC CACATGGTGA TGCAGAAGCT CCTCCGCAAG
    CAGGAGTCCA CAGTGATGGT TCTGCGTAAC ATGGTGGACC CCAAGGACAT CGACGATGAC
    CTGGAAGGGG AGGTGACTGA GGAGTGTGGC AAGTTTGGTG CTGTCAACCG TGTCATCATC
    TACCAAGAGA AGCAGGGCGA GGAAGAGGAC GCGGAGATCA TTGTCAAGAT TTTTGTGGAG
    TTTTCCGTAG CCTCTGAGAC TCACAAGGCC ATCCAGGCCC TCAATGGGCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTATGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25745
    Clone ID Related Accession (Same CDS sequence) XM_005215170.4 , XM_005215170.3
    Accession Version XM_005215170.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCAG CAGTGACAAT GGGCTTTGGA GATCCTCTCT CACCTTTGCA ATCGATGGCG
    GCTCAGCGGC AGCGGGCACT GGCCATCATG TGCCGGGTCT ACGTGGGCTC CATCTACTAC
    GAGCTAGGGG AGGACACCAT CCGCCAGGCG TTCGCCCCCT TTGGGCCTAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCGTTTGT GGAGTATGAG
    GTCCCAGAAG CCGCCCAGCT GGCCTTGGAG CAGATGAATT CCGTGATGCT GGGAGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCTC AGCCCATCAT AGACCAGCTG
    GCCGAGGAGG CGCGAGCCTT CAACCGCATC TACGTGGCTT CCGTGCACCA GGACCTCTCG
    GATGATGACA TCAAGAGTGT GTTTGAGGCC TTTGGCAAGA TCAAATCCTG CACGCTGGCT
    CGGGACCCCA CGACTGGCAA ACACAAGGGC TATGGCTTCA TTGAATATGA GAAAGCCCAA
    TCGTCCCAGG ATGCTGTGTC TTCCATGAAC CTTTTTGACC TGGGGGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC CCCTATGCCC CTGCTTACAC CGGCCACACC TGGAGGCCTC
    CCGCCGGCTG CTGCCGTGGC CGCAGCTGCA GCCACAGCCA AGATTACAGC TCAGGAAGCA
    GTGGCTGGAG CAGCAGTACT GGGTACCCTA GCCACTCCTG GACTGGTGTC CCCTGCACTG
    ACTCTGGCCC AGCCCCTGGG GGCTCTACCC CAGGCCGTCA TGGCTGCCCA GGCCCCAGGA
    GTCATCACAG GTGTGACACC GGCTCGGCCT CCCATTCCGG TCACCATCCC CTCTGTTGGT
    GTGGTGAATC CCATCCTGGC CAGCCCTCCG ACACTTGGTC TCCTGGAGCC TAAGAAGGAG
    AAGGAGGAGG AGGAGCTGTT TCCCGAGTCG GAGCGGCCGG AGATGCTGAG TGAGCAGGAG
    CACATGAGCA TCTCAGGAAG CAGCGCCCGC CACATGGTGA TGCAGAAGCT CCTCCGCAAG
    CAGGAGTCCA CAGTGATGGT TCTGCGTAAC ATGGTGGACC CCAAGGACAT CGACGATGAC
    CTGGAAGGGG AGGTGACTGA GGAGTGTGGC AAGTTTGGTG CTGTCAACCG TGTCATCATC
    TACCAAGAGA AGCAGGGCGA GGAAGAGGAC GCGGAGATCA TTGTCAAGAT TTTTGTGGAG
    TTTTCCGTAG CCTCTGAGAC TCACAAGGCC ATCCAGGCCC TCAATGGGCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTATGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215227.1
    CDS190..1875
    Translation

    Target ORF information:

    RefSeq Version XM_005215170.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCAG CAGTGACAAT GGGCTTTGGA GATCCTCTCT CACCTTTGCA ATCGATGGCG
    GCTCAGCGGC AGCGGGCACT GGCCATCATG TGCCGGGTCT ACGTGGGCTC CATCTACTAC
    GAGCTAGGGG AGGACACCAT CCGCCAGGCG TTCGCCCCCT TTGGGCCTAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCGTTTGT GGAGTATGAG
    GTCCCAGAAG CCGCCCAGCT GGCCTTGGAG CAGATGAATT CCGTGATGCT GGGAGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCTC AGCCCATCAT AGACCAGCTG
    GCCGAGGAGG CGCGAGCCTT CAACCGCATC TACGTGGCTT CCGTGCACCA GGACCTCTCG
    GATGATGACA TCAAGAGTGT GTTTGAGGCC TTTGGCAAGA TCAAATCCTG CACGCTGGCT
    CGGGACCCCA CGACTGGCAA ACACAAGGGC TATGGCTTCA TTGAATATGA GAAAGCCCAA
    TCGTCCCAGG ATGCTGTGTC TTCCATGAAC CTTTTTGACC TGGGGGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC CCCTATGCCC CTGCTTACAC CGGCCACACC TGGAGGCCTC
    CCGCCGGCTG CTGCCGTGGC CGCAGCTGCA GCCACAGCCA AGATTACAGC TCAGGAAGCA
    GTGGCTGGAG CAGCAGTACT GGGTACCCTA GCCACTCCTG GACTGGTGTC CCCTGCACTG
    ACTCTGGCCC AGCCCCTGGG GGCTCTACCC CAGGCCGTCA TGGCTGCCCA GGCCCCAGGA
    GTCATCACAG GTGTGACACC GGCTCGGCCT CCCATTCCGG TCACCATCCC CTCTGTTGGT
    GTGGTGAATC CCATCCTGGC CAGCCCTCCG ACACTTGGTC TCCTGGAGCC TAAGAAGGAG
    AAGGAGGAGG AGGAGCTGTT TCCCGAGTCG GAGCGGCCGG AGATGCTGAG TGAGCAGGAG
    CACATGAGCA TCTCAGGAAG CAGCGCCCGC CACATGGTGA TGCAGAAGCT CCTCCGCAAG
    CAGGAGTCCA CAGTGATGGT TCTGCGTAAC ATGGTGGACC CCAAGGACAT CGACGATGAC
    CTGGAAGGGG AGGTGACTGA GGAGTGTGGC AAGTTTGGTG CTGTCAACCG TGTCATCATC
    TACCAAGAGA AGCAGGGCGA GGAAGAGGAC GCGGAGATCA TTGTCAAGAT TTTTGTGGAG
    TTTTCCGTAG CCTCTGAGAC TCACAAGGCC ATCCAGGCCC TCAATGGGCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTATGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25746
    Clone ID Related Accession (Same CDS sequence) NM_001359973.1 , XM_005215171.3
    Accession Version XM_005215171.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CGGCTCAGCG GCAGCGGGCA CTGGCCATCA TGTGCCGGGT CTACGTGGGC
    TCCATCTACT ACGAGCTAGG GGAGGACACC ATCCGCCAGG CGTTCGCCCC CTTTGGGCCT
    ATCAAGAGCA TCGACATGTC CTGGGACTCC GTCACCATGA AGCACAAGGG CTTTGCGTTT
    GTGGAGTATG AGGTCCCAGA AGCCGCCCAG CTGGCCTTGG AGCAGATGAA TTCCGTGATG
    CTGGGAGGCA GGAACATCAA GGTGGGCAGA CCCAGCAACA TAGGGCAGGC TCAGCCCATC
    ATAGACCAGC TGGCCGAGGA GGCGCGAGCC TTCAACCGCA TCTACGTGGC TTCCGTGCAC
    CAGGACCTCT CGGATGATGA CATCAAGAGT GTGTTTGAGG CCTTTGGCAA GATCAAATCC
    TGCACGCTGG CTCGGGACCC CACGACTGGC AAACACAAGG GCTATGGCTT CATTGAATAT
    GAGAAAGCCC AATCGTCCCA GGATGCTGTG TCTTCCATGA ACCTTTTTGA CCTGGGGGGC
    CAGTACTTGC GGGTGGGCAA GGCTGTCACA CCCCCTATGC CCCTGCTTAC ACCGGCCACA
    CCTGGAGGCC TCCCGCCGGC TGCTGCCGTG GCCGCAGCTG CAGCCACAGC CAAGATTACA
    GCTCAGGAAG CAGTGGCTGG AGCAGCAGTA CTGGGTACCC TAGCCACTCC TGGACTGGTG
    TCCCCTGCAC TGACTCTGGC CCAGCCCCTG GGGGCTCTAC CCCAGGCCGT CATGGCTGCC
    CAGGCCCCAG GAGTCATCAC AGGTGTGACA CCGGCTCGGC CTCCCATTCC GGTCACCATC
    CCCTCTGTTG GTGTGGTGAA TCCCATCCTG GCCAGCCCTC CGACACTTGG TCTCCTGGAG
    CCTAAGAAGG AGAAGGAGGA GGAGGAGCTG TTTCCCGAGT CGGAGCGGCC GGAGATGCTG
    AGTGAGCAGG AGCACATGAG CATCTCAGGA AGCAGCGCCC GCCACATGGT GATGCAGAAG
    CTCCTCCGCA AGCAGGAGTC CACAGTGATG GTTCTGCGTA ACATGGTGGA CCCCAAGGAC
    ATCGACGATG ACCTGGAAGG GGAGGTGACT GAGGAGTGTG GCAAGTTTGG TGCTGTCAAC
    CGTGTCATCA TCTACCAAGA GAAGCAGGGC GAGGAAGAGG ACGCGGAGAT CATTGTCAAG
    ATTTTTGTGG AGTTTTCCGT AGCCTCTGAG ACTCACAAGG CCATCCAGGC CCTCAATGGG
    CGCTGGTTTG CTGGCCGCAA GGTGGTGGCT GAAGTGTATG ACCAGGAGCG TTTTGATAAC
    AGTGACCTCT CTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215228.1
    CDS189..1826
    Translation

    Target ORF information:

    RefSeq Version XM_005215171.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215171.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTACAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCAGCAGCGG CGGCGGCGGC GGCAGTGGTG GCAGCGGGAG ACAAATGGAA ACCTCCACAG
    GGGACAGACT CCATCAAGAT GGAGAACGGG CAGGGCACAG CCGCGAAGCT GGGACTGCCT
    CCCTTGACGC CCGAGCAGCA GGAGGCCCTC CAGAAGGCCA AGAAGTACGC CATGGAGCAG
    AGCATCAAGA GCGTGCTGGT GAAACAGACC ATCGCCCACC AGCAGCAGCA GCTTACCAAC
    CTGCAGATGG CGGCTCAGCG GCAGCGGGCA CTGGCCATCA TGTGCCGGGT CTACGTGGGC
    TCCATCTACT ACGAGCTAGG GGAGGACACC ATCCGCCAGG CGTTCGCCCC CTTTGGGCCT
    ATCAAGAGCA TCGACATGTC CTGGGACTCC GTCACCATGA AGCACAAGGG CTTTGCGTTT
    GTGGAGTATG AGGTCCCAGA AGCCGCCCAG CTGGCCTTGG AGCAGATGAA TTCCGTGATG
    CTGGGAGGCA GGAACATCAA GGTGGGCAGA CCCAGCAACA TAGGGCAGGC TCAGCCCATC
    ATAGACCAGC TGGCCGAGGA GGCGCGAGCC TTCAACCGCA TCTACGTGGC TTCCGTGCAC
    CAGGACCTCT CGGATGATGA CATCAAGAGT GTGTTTGAGG CCTTTGGCAA GATCAAATCC
    TGCACGCTGG CTCGGGACCC CACGACTGGC AAACACAAGG GCTATGGCTT CATTGAATAT
    GAGAAAGCCC AATCGTCCCA GGATGCTGTG TCTTCCATGA ACCTTTTTGA CCTGGGGGGC
    CAGTACTTGC GGGTGGGCAA GGCTGTCACA CCCCCTATGC CCCTGCTTAC ACCGGCCACA
    CCTGGAGGCC TCCCGCCGGC TGCTGCCGTG GCCGCAGCTG CAGCCACAGC CAAGATTACA
    GCTCAGGAAG CAGTGGCTGG AGCAGCAGTA CTGGGTACCC TAGCCACTCC TGGACTGGTG
    TCCCCTGCAC TGACTCTGGC CCAGCCCCTG GGGGCTCTAC CCCAGGCCGT CATGGCTGCC
    CAGGCCCCAG GAGTCATCAC AGGTGTGACA CCGGCTCGGC CTCCCATTCC GGTCACCATC
    CCCTCTGTTG GTGTGGTGAA TCCCATCCTG GCCAGCCCTC CGACACTTGG TCTCCTGGAG
    CCTAAGAAGG AGAAGGAGGA GGAGGAGCTG TTTCCCGAGT CGGAGCGGCC GGAGATGCTG
    AGTGAGCAGG AGCACATGAG CATCTCAGGA AGCAGCGCCC GCCACATGGT GATGCAGAAG
    CTCCTCCGCA AGCAGGAGTC CACAGTGATG GTTCTGCGTA ACATGGTGGA CCCCAAGGAC
    ATCGACGATG ACCTGGAAGG GGAGGTGACT GAGGAGTGTG GCAAGTTTGG TGCTGTCAAC
    CGTGTCATCA TCTACCAAGA GAAGCAGGGC GAGGAAGAGG ACGCGGAGAT CATTGTCAAG
    ATTTTTGTGG AGTTTTCCGT AGCCTCTGAG ACTCACAAGG CCATCCAGGC CCTCAATGGG
    CGCTGGTTTG CTGGCCGCAA GGTGGTGGCT GAAGTGTATG ACCAGGAGCG TTTTGATAAC
    AGTGACCTCT CTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25747
    Clone ID Related Accession (Same CDS sequence) XM_005215172.4 , XM_005215172.3
    Accession Version XM_005215172.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCGG CTCAGCGGCA GCGGGCACTG GCCATCATGT GCCGGGTCTA CGTGGGCTCC
    ATCTACTACG AGCTAGGGGA GGACACCATC CGCCAGGCGT TCGCCCCCTT TGGGCCTATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCGTTTGTG
    GAGTATGAGG TCCCAGAAGC CGCCCAGCTG GCCTTGGAGC AGATGAATTC CGTGATGCTG
    GGAGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCTCA GCCCATCATA
    GACCAGCTGG CCGAGGAGGC GCGAGCCTTC AACCGCATCT ACGTGGCTTC CGTGCACCAG
    GACCTCTCGG ATGATGACAT CAAGAGTGTG TTTGAGGCCT TTGGCAAGAT CAAATCCTGC
    ACGCTGGCTC GGGACCCCAC GACTGGCAAA CACAAGGGCT ATGGCTTCAT TGAATATGAG
    AAAGCCCAAT CGTCCCAGGA TGCTGTGTCT TCCATGAACC TTTTTGACCT GGGGGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCC CCTATGCCCC TGCTTACACC GGCCACACCT
    GGAGGCCTCC CGCCGGCTGC TGCCGTGGCC GCAGCTGCAG CCACAGCCAA GATTACAGCT
    CAGGAAGCAG TGGCTGGAGC AGCAGTACTG GGTACCCTAG CCACTCCTGG ACTGGTGTCC
    CCTGCACTGA CTCTGGCCCA GCCCCTGGGG GCTCTACCCC AGGCCGTCAT GGCTGCCCAG
    GCCCCAGGAG TCATCACAGG TGTGACACCG GCTCGGCCTC CCATTCCGGT CACCATCCCC
    TCTGTTGGTG TGGTGAATCC CATCCTGGCC AGCCCTCCGA CACTTGGTCT CCTGGAGCCT
    AAGAAGGAGA AGGAGGAGGA GGAGCTGTTT CCCGAGTCGG AGCGGCCGGA GATGCTGAGT
    GAGCAGGAGC ACATGAGCAT CTCAGGAAGC AGCGCCCGCC ACATGGTGAT GCAGAAGCTC
    CTCCGCAAGC AGGAGTCCAC AGTGATGGTT CTGCGTAACA TGGTGGACCC CAAGGACATC
    GACGATGACC TGGAAGGGGA GGTGACTGAG GAGTGTGGCA AGTTTGGTGC TGTCAACCGT
    GTCATCATCT ACCAAGAGAA GCAGGGCGAG GAAGAGGACG CGGAGATCAT TGTCAAGATT
    TTTGTGGAGT TTTCCGTAGC CTCTGAGACT CACAAGGCCA TCCAGGCCCT CAATGGGCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTATGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215229.1
    CDS145..1779
    Translation

    Target ORF information:

    RefSeq Version XM_005215172.4
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215172.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCGG CTCAGCGGCA GCGGGCACTG GCCATCATGT GCCGGGTCTA CGTGGGCTCC
    ATCTACTACG AGCTAGGGGA GGACACCATC CGCCAGGCGT TCGCCCCCTT TGGGCCTATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCGTTTGTG
    GAGTATGAGG TCCCAGAAGC CGCCCAGCTG GCCTTGGAGC AGATGAATTC CGTGATGCTG
    GGAGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCTCA GCCCATCATA
    GACCAGCTGG CCGAGGAGGC GCGAGCCTTC AACCGCATCT ACGTGGCTTC CGTGCACCAG
    GACCTCTCGG ATGATGACAT CAAGAGTGTG TTTGAGGCCT TTGGCAAGAT CAAATCCTGC
    ACGCTGGCTC GGGACCCCAC GACTGGCAAA CACAAGGGCT ATGGCTTCAT TGAATATGAG
    AAAGCCCAAT CGTCCCAGGA TGCTGTGTCT TCCATGAACC TTTTTGACCT GGGGGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCC CCTATGCCCC TGCTTACACC GGCCACACCT
    GGAGGCCTCC CGCCGGCTGC TGCCGTGGCC GCAGCTGCAG CCACAGCCAA GATTACAGCT
    CAGGAAGCAG TGGCTGGAGC AGCAGTACTG GGTACCCTAG CCACTCCTGG ACTGGTGTCC
    CCTGCACTGA CTCTGGCCCA GCCCCTGGGG GCTCTACCCC AGGCCGTCAT GGCTGCCCAG
    GCCCCAGGAG TCATCACAGG TGTGACACCG GCTCGGCCTC CCATTCCGGT CACCATCCCC
    TCTGTTGGTG TGGTGAATCC CATCCTGGCC AGCCCTCCGA CACTTGGTCT CCTGGAGCCT
    AAGAAGGAGA AGGAGGAGGA GGAGCTGTTT CCCGAGTCGG AGCGGCCGGA GATGCTGAGT
    GAGCAGGAGC ACATGAGCAT CTCAGGAAGC AGCGCCCGCC ACATGGTGAT GCAGAAGCTC
    CTCCGCAAGC AGGAGTCCAC AGTGATGGTT CTGCGTAACA TGGTGGACCC CAAGGACATC
    GACGATGACC TGGAAGGGGA GGTGACTGAG GAGTGTGGCA AGTTTGGTGC TGTCAACCGT
    GTCATCATCT ACCAAGAGAA GCAGGGCGAG GAAGAGGACG CGGAGATCAT TGTCAAGATT
    TTTGTGGAGT TTTCCGTAGC CTCTGAGACT CACAAGGCCA TCCAGGCCCT CAATGGGCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTATGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25747
    Clone ID Related Accession (Same CDS sequence) XM_005215172.4 , XM_005215172.3
    Accession Version XM_005215172.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCGG CTCAGCGGCA GCGGGCACTG GCCATCATGT GCCGGGTCTA CGTGGGCTCC
    ATCTACTACG AGCTAGGGGA GGACACCATC CGCCAGGCGT TCGCCCCCTT TGGGCCTATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCGTTTGTG
    GAGTATGAGG TCCCAGAAGC CGCCCAGCTG GCCTTGGAGC AGATGAATTC CGTGATGCTG
    GGAGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCTCA GCCCATCATA
    GACCAGCTGG CCGAGGAGGC GCGAGCCTTC AACCGCATCT ACGTGGCTTC CGTGCACCAG
    GACCTCTCGG ATGATGACAT CAAGAGTGTG TTTGAGGCCT TTGGCAAGAT CAAATCCTGC
    ACGCTGGCTC GGGACCCCAC GACTGGCAAA CACAAGGGCT ATGGCTTCAT TGAATATGAG
    AAAGCCCAAT CGTCCCAGGA TGCTGTGTCT TCCATGAACC TTTTTGACCT GGGGGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCC CCTATGCCCC TGCTTACACC GGCCACACCT
    GGAGGCCTCC CGCCGGCTGC TGCCGTGGCC GCAGCTGCAG CCACAGCCAA GATTACAGCT
    CAGGAAGCAG TGGCTGGAGC AGCAGTACTG GGTACCCTAG CCACTCCTGG ACTGGTGTCC
    CCTGCACTGA CTCTGGCCCA GCCCCTGGGG GCTCTACCCC AGGCCGTCAT GGCTGCCCAG
    GCCCCAGGAG TCATCACAGG TGTGACACCG GCTCGGCCTC CCATTCCGGT CACCATCCCC
    TCTGTTGGTG TGGTGAATCC CATCCTGGCC AGCCCTCCGA CACTTGGTCT CCTGGAGCCT
    AAGAAGGAGA AGGAGGAGGA GGAGCTGTTT CCCGAGTCGG AGCGGCCGGA GATGCTGAGT
    GAGCAGGAGC ACATGAGCAT CTCAGGAAGC AGCGCCCGCC ACATGGTGAT GCAGAAGCTC
    CTCCGCAAGC AGGAGTCCAC AGTGATGGTT CTGCGTAACA TGGTGGACCC CAAGGACATC
    GACGATGACC TGGAAGGGGA GGTGACTGAG GAGTGTGGCA AGTTTGGTGC TGTCAACCGT
    GTCATCATCT ACCAAGAGAA GCAGGGCGAG GAAGAGGACG CGGAGATCAT TGTCAAGATT
    TTTGTGGAGT TTTCCGTAGC CTCTGAGACT CACAAGGCCA TCCAGGCCCT CAATGGGCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTATGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215229.1
    CDS189..1823
    Translation

    Target ORF information:

    RefSeq Version XM_005215172.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC GCTAGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCA 
    GCAGCGGCGG CGGCGGCGGC AGTGGTGGCA GCGGGAGACA AATGGAAACC TCCACAGGGG
    ACAGACTCCA TCAAGATGGA GAACGGGCAG GGCACAGCCG CGAAGCTGGG ACTGCCTCCC
    TTGACGCCCG AGCAGCAGGA GGCCCTCCAG AAGGCCAAGA AGTACGCCAT GGAGCAGAGC
    ATCAAGAGCG TGCTGGTGAA ACAGACCATC GCCCACCAGC AGCAGCAGCT TACCAACCTG
    CAGATGGCGG CTCAGCGGCA GCGGGCACTG GCCATCATGT GCCGGGTCTA CGTGGGCTCC
    ATCTACTACG AGCTAGGGGA GGACACCATC CGCCAGGCGT TCGCCCCCTT TGGGCCTATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCGTTTGTG
    GAGTATGAGG TCCCAGAAGC CGCCCAGCTG GCCTTGGAGC AGATGAATTC CGTGATGCTG
    GGAGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCTCA GCCCATCATA
    GACCAGCTGG CCGAGGAGGC GCGAGCCTTC AACCGCATCT ACGTGGCTTC CGTGCACCAG
    GACCTCTCGG ATGATGACAT CAAGAGTGTG TTTGAGGCCT TTGGCAAGAT CAAATCCTGC
    ACGCTGGCTC GGGACCCCAC GACTGGCAAA CACAAGGGCT ATGGCTTCAT TGAATATGAG
    AAAGCCCAAT CGTCCCAGGA TGCTGTGTCT TCCATGAACC TTTTTGACCT GGGGGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCC CCTATGCCCC TGCTTACACC GGCCACACCT
    GGAGGCCTCC CGCCGGCTGC TGCCGTGGCC GCAGCTGCAG CCACAGCCAA GATTACAGCT
    CAGGAAGCAG TGGCTGGAGC AGCAGTACTG GGTACCCTAG CCACTCCTGG ACTGGTGTCC
    CCTGCACTGA CTCTGGCCCA GCCCCTGGGG GCTCTACCCC AGGCCGTCAT GGCTGCCCAG
    GCCCCAGGAG TCATCACAGG TGTGACACCG GCTCGGCCTC CCATTCCGGT CACCATCCCC
    TCTGTTGGTG TGGTGAATCC CATCCTGGCC AGCCCTCCGA CACTTGGTCT CCTGGAGCCT
    AAGAAGGAGA AGGAGGAGGA GGAGCTGTTT CCCGAGTCGG AGCGGCCGGA GATGCTGAGT
    GAGCAGGAGC ACATGAGCAT CTCAGGAAGC AGCGCCCGCC ACATGGTGAT GCAGAAGCTC
    CTCCGCAAGC AGGAGTCCAC AGTGATGGTT CTGCGTAACA TGGTGGACCC CAAGGACATC
    GACGATGACC TGGAAGGGGA GGTGACTGAG GAGTGTGGCA AGTTTGGTGC TGTCAACCGT
    GTCATCATCT ACCAAGAGAA GCAGGGCGAG GAAGAGGACG CGGAGATCAT TGTCAAGATT
    TTTGTGGAGT TTTCCGTAGC CTCTGAGACT CACAAGGCCA TCCAGGCCCT CAATGGGCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTATGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25748
    Clone ID Related Accession (Same CDS sequence) NM_001359974.1 , XM_005215173.3
    Accession Version XM_005215173.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCACTGGCC
    ATCATGTGCC GGGTCTACGT GGGCTCCATC TACTACGAGC TAGGGGAGGA CACCATCCGC
    CAGGCGTTCG CCCCCTTTGG GCCTATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC GTTTGTGGAG TATGAGGTCC CAGAAGCCGC CCAGCTGGCC
    TTGGAGCAGA TGAATTCCGT GATGCTGGGA GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCTCAGCC CATCATAGAC CAGCTGGCCG AGGAGGCGCG AGCCTTCAAC
    CGCATCTACG TGGCTTCCGT GCACCAGGAC CTCTCGGATG ATGACATCAA GAGTGTGTTT
    GAGGCCTTTG GCAAGATCAA ATCCTGCACG CTGGCTCGGG ACCCCACGAC TGGCAAACAC
    AAGGGCTATG GCTTCATTGA ATATGAGAAA GCCCAATCGT CCCAGGATGC TGTGTCTTCC
    ATGAACCTTT TTGACCTGGG GGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCCCCT
    ATGCCCCTGC TTACACCGGC CACACCTGGA GGCCTCCCGC CGGCTGCTGC CGTGGCCGCA
    GCTGCAGCCA CAGCCAAGAT TACAGCTCAG GAAGCAGTGG CTGGAGCAGC AGTACTGGGT
    ACCCTAGCCA CTCCTGGACT GGTGTCCCCT GCACTGACTC TGGCCCAGCC CCTGGGGGCT
    CTACCCCAGG CCGTCATGGC TGCCCAGGCC CCAGGAGTCA TCACAGGTGT GACACCGGCT
    CGGCCTCCCA TTCCGGTCAC CATCCCCTCT GTTGGTGTGG TGAATCCCAT CCTGGCCAGC
    CCTCCGACAC TTGGTCTCCT GGAGCCTAAG AAGGAGAAGG AGGAGGAGGA GCTGTTTCCC
    GAGTCGGAGC GGCCGGAGAT GCTGAGTGAG CAGGAGCACA TGAGCATCTC AGGAAGCAGC
    GCCCGCCACA TGGTGATGCA GAAGCTCCTC CGCAAGCAGG AGTCCACAGT GATGGTTCTG
    CGTAACATGG TGGACCCCAA GGACATCGAC GATGACCTGG AAGGGGAGGT GACTGAGGAG
    TGTGGCAAGT TTGGTGCTGT CAACCGTGTC ATCATCTACC AAGAGAAGCA GGGCGAGGAA
    GAGGACGCGG AGATCATTGT CAAGATTTTT GTGGAGTTTT CCGTAGCCTC TGAGACTCAC
    AAGGCCATCC AGGCCCTCAA TGGGCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TATGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215230.1
    CDS198..1739
    Translation

    Target ORF information:

    RefSeq Version XM_005215173.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCACTGGCC
    ATCATGTGCC GGGTCTACGT GGGCTCCATC TACTACGAGC TAGGGGAGGA CACCATCCGC
    CAGGCGTTCG CCCCCTTTGG GCCTATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC GTTTGTGGAG TATGAGGTCC CAGAAGCCGC CCAGCTGGCC
    TTGGAGCAGA TGAATTCCGT GATGCTGGGA GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCTCAGCC CATCATAGAC CAGCTGGCCG AGGAGGCGCG AGCCTTCAAC
    CGCATCTACG TGGCTTCCGT GCACCAGGAC CTCTCGGATG ATGACATCAA GAGTGTGTTT
    GAGGCCTTTG GCAAGATCAA ATCCTGCACG CTGGCTCGGG ACCCCACGAC TGGCAAACAC
    AAGGGCTATG GCTTCATTGA ATATGAGAAA GCCCAATCGT CCCAGGATGC TGTGTCTTCC
    ATGAACCTTT TTGACCTGGG GGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCCCCT
    ATGCCCCTGC TTACACCGGC CACACCTGGA GGCCTCCCGC CGGCTGCTGC CGTGGCCGCA
    GCTGCAGCCA CAGCCAAGAT TACAGCTCAG GAAGCAGTGG CTGGAGCAGC AGTACTGGGT
    ACCCTAGCCA CTCCTGGACT GGTGTCCCCT GCACTGACTC TGGCCCAGCC CCTGGGGGCT
    CTACCCCAGG CCGTCATGGC TGCCCAGGCC CCAGGAGTCA TCACAGGTGT GACACCGGCT
    CGGCCTCCCA TTCCGGTCAC CATCCCCTCT GTTGGTGTGG TGAATCCCAT CCTGGCCAGC
    CCTCCGACAC TTGGTCTCCT GGAGCCTAAG AAGGAGAAGG AGGAGGAGGA GCTGTTTCCC
    GAGTCGGAGC GGCCGGAGAT GCTGAGTGAG CAGGAGCACA TGAGCATCTC AGGAAGCAGC
    GCCCGCCACA TGGTGATGCA GAAGCTCCTC CGCAAGCAGG AGTCCACAGT GATGGTTCTG
    CGTAACATGG TGGACCCCAA GGACATCGAC GATGACCTGG AAGGGGAGGT GACTGAGGAG
    TGTGGCAAGT TTGGTGCTGT CAACCGTGTC ATCATCTACC AAGAGAAGCA GGGCGAGGAA
    GAGGACGCGG AGATCATTGT CAAGATTTTT GTGGAGTTTT CCGTAGCCTC TGAGACTCAC
    AAGGCCATCC AGGCCCTCAA TGGGCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TATGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48446
    Clone ID Related Accession (Same CDS sequence) XM_010811834.2 , XM_010811834.3
    Accession Version XM_010811834.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CACTGGCCAT CATGTGCCGG GTCTACGTGG GCTCCATCTA CTACGAGCTA
    GGGGAGGACA CCATCCGCCA GGCGTTCGCC CCCTTTGGGC CTATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCGT TTGTGGAGTA TGAGGTCCCA
    GAAGCCGCCC AGCTGGCCTT GGAGCAGATG AATTCCGTGA TGCTGGGAGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCTCAGCCCA TCATAGACCA GCTGGCCGAG
    GAGGCGCGAG CCTTCAACCG CATCTACGTG GCTTCCGTGC ACCAGGACCT CTCGGATGAT
    GACATCAAGA GTGTGTTTGA GGCCTTTGGC AAGATCAAAT CCTGCACGCT GGCTCGGGAC
    CCCACGACTG GCAAACACAA GGGCTATGGC TTCATTGAAT ATGAGAAAGC CCAATCGTCC
    CAGGATGCTG TGTCTTCCAT GAACCTTTTT GACCTGGGGG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCCCCTAT GCCCCTGCTT ACACCGGCCA CACCTGGAGG CCTCCCGCCG
    GCTGCTGCCG TGGCCGCAGC TGCAGCCACA GCCAAGATTA CAGCTCAGGA AGCAGTGGCT
    GGAGCAGCAG TACTGGGTAC CCTAGCCACT CCTGGACTGG TGTCCCCTGC ACTGACTCTG
    GCCCAGCCCC TGGGGGCTCT ACCCCAGGCC GTCATGGCTG CCCAGGCCCC AGGAGTCATC
    ACAGGTGTGA CACCGGCTCG GCCTCCCATT CCGGTCACCA TCCCCTCTGT TGGTGTGGTG
    AATCCCATCC TGGCCAGCCC TCCGACACTT GGTCTCCTGG AGCCTAAGAA GGAGAAGGAG
    GAGGAGGAGC TGTTTCCCGA GTCGGAGCGG CCGGAGATGC TGAGTGAGCA GGAGCACATG
    AGCATCTCAG GAAGCAGCGC CCGCCACATG GTGATGCAGA AGCTCCTCCG CAAGCAGGAG
    TCCACAGTGA TGGTTCTGCG TAACATGGTG GACCCCAAGG ACATCGACGA TGACCTGGAA
    GGGGAGGTGA CTGAGGAGTG TGGCAAGTTT GGTGCTGTCA ACCGTGTCAT CATCTACCAA
    GAGAAGCAGG GCGAGGAAGA GGACGCGGAG ATCATTGTCA AGATTTTTGT GGAGTTTTCC
    GTAGCCTCTG AGACTCACAA GGCCATCCAG GCCCTCAATG GGCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA TGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810136.1
    CDS3152..4651
    Translation

    Target ORF information:

    RefSeq Version XM_010811834.2
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811834.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CACTGGCCAT CATGTGCCGG GTCTACGTGG GCTCCATCTA CTACGAGCTA
    GGGGAGGACA CCATCCGCCA GGCGTTCGCC CCCTTTGGGC CTATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCGT TTGTGGAGTA TGAGGTCCCA
    GAAGCCGCCC AGCTGGCCTT GGAGCAGATG AATTCCGTGA TGCTGGGAGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCTCAGCCCA TCATAGACCA GCTGGCCGAG
    GAGGCGCGAG CCTTCAACCG CATCTACGTG GCTTCCGTGC ACCAGGACCT CTCGGATGAT
    GACATCAAGA GTGTGTTTGA GGCCTTTGGC AAGATCAAAT CCTGCACGCT GGCTCGGGAC
    CCCACGACTG GCAAACACAA GGGCTATGGC TTCATTGAAT ATGAGAAAGC CCAATCGTCC
    CAGGATGCTG TGTCTTCCAT GAACCTTTTT GACCTGGGGG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCCCCTAT GCCCCTGCTT ACACCGGCCA CACCTGGAGG CCTCCCGCCG
    GCTGCTGCCG TGGCCGCAGC TGCAGCCACA GCCAAGATTA CAGCTCAGGA AGCAGTGGCT
    GGAGCAGCAG TACTGGGTAC CCTAGCCACT CCTGGACTGG TGTCCCCTGC ACTGACTCTG
    GCCCAGCCCC TGGGGGCTCT ACCCCAGGCC GTCATGGCTG CCCAGGCCCC AGGAGTCATC
    ACAGGTGTGA CACCGGCTCG GCCTCCCATT CCGGTCACCA TCCCCTCTGT TGGTGTGGTG
    AATCCCATCC TGGCCAGCCC TCCGACACTT GGTCTCCTGG AGCCTAAGAA GGAGAAGGAG
    GAGGAGGAGC TGTTTCCCGA GTCGGAGCGG CCGGAGATGC TGAGTGAGCA GGAGCACATG
    AGCATCTCAG GAAGCAGCGC CCGCCACATG GTGATGCAGA AGCTCCTCCG CAAGCAGGAG
    TCCACAGTGA TGGTTCTGCG TAACATGGTG GACCCCAAGG ACATCGACGA TGACCTGGAA
    GGGGAGGTGA CTGAGGAGTG TGGCAAGTTT GGTGCTGTCA ACCGTGTCAT CATCTACCAA
    GAGAAGCAGG GCGAGGAAGA GGACGCGGAG ATCATTGTCA AGATTTTTGT GGAGTTTTCC
    GTAGCCTCTG AGACTCACAA GGCCATCCAG GCCCTCAATG GGCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA TGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48446
    Clone ID Related Accession (Same CDS sequence) XM_010811834.2 , XM_010811834.3
    Accession Version XM_010811834.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CACTGGCCAT CATGTGCCGG GTCTACGTGG GCTCCATCTA CTACGAGCTA
    GGGGAGGACA CCATCCGCCA GGCGTTCGCC CCCTTTGGGC CTATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCGT TTGTGGAGTA TGAGGTCCCA
    GAAGCCGCCC AGCTGGCCTT GGAGCAGATG AATTCCGTGA TGCTGGGAGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCTCAGCCCA TCATAGACCA GCTGGCCGAG
    GAGGCGCGAG CCTTCAACCG CATCTACGTG GCTTCCGTGC ACCAGGACCT CTCGGATGAT
    GACATCAAGA GTGTGTTTGA GGCCTTTGGC AAGATCAAAT CCTGCACGCT GGCTCGGGAC
    CCCACGACTG GCAAACACAA GGGCTATGGC TTCATTGAAT ATGAGAAAGC CCAATCGTCC
    CAGGATGCTG TGTCTTCCAT GAACCTTTTT GACCTGGGGG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCCCCTAT GCCCCTGCTT ACACCGGCCA CACCTGGAGG CCTCCCGCCG
    GCTGCTGCCG TGGCCGCAGC TGCAGCCACA GCCAAGATTA CAGCTCAGGA AGCAGTGGCT
    GGAGCAGCAG TACTGGGTAC CCTAGCCACT CCTGGACTGG TGTCCCCTGC ACTGACTCTG
    GCCCAGCCCC TGGGGGCTCT ACCCCAGGCC GTCATGGCTG CCCAGGCCCC AGGAGTCATC
    ACAGGTGTGA CACCGGCTCG GCCTCCCATT CCGGTCACCA TCCCCTCTGT TGGTGTGGTG
    AATCCCATCC TGGCCAGCCC TCCGACACTT GGTCTCCTGG AGCCTAAGAA GGAGAAGGAG
    GAGGAGGAGC TGTTTCCCGA GTCGGAGCGG CCGGAGATGC TGAGTGAGCA GGAGCACATG
    AGCATCTCAG GAAGCAGCGC CCGCCACATG GTGATGCAGA AGCTCCTCCG CAAGCAGGAG
    TCCACAGTGA TGGTTCTGCG TAACATGGTG GACCCCAAGG ACATCGACGA TGACCTGGAA
    GGGGAGGTGA CTGAGGAGTG TGGCAAGTTT GGTGCTGTCA ACCGTGTCAT CATCTACCAA
    GAGAAGCAGG GCGAGGAAGA GGACGCGGAG ATCATTGTCA AGATTTTTGT GGAGTTTTCC
    GTAGCCTCTG AGACTCACAA GGCCATCCAG GCCCTCAATG GGCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA TGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810136.1
    CDS3240..4739
    Translation

    Target ORF information:

    RefSeq Version XM_010811834.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811834.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CACTGGCCAT CATGTGCCGG GTCTACGTGG GCTCCATCTA CTACGAGCTA
    GGGGAGGACA CCATCCGCCA GGCGTTCGCC CCCTTTGGGC CTATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCGT TTGTGGAGTA TGAGGTCCCA
    GAAGCCGCCC AGCTGGCCTT GGAGCAGATG AATTCCGTGA TGCTGGGAGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCTCAGCCCA TCATAGACCA GCTGGCCGAG
    GAGGCGCGAG CCTTCAACCG CATCTACGTG GCTTCCGTGC ACCAGGACCT CTCGGATGAT
    GACATCAAGA GTGTGTTTGA GGCCTTTGGC AAGATCAAAT CCTGCACGCT GGCTCGGGAC
    CCCACGACTG GCAAACACAA GGGCTATGGC TTCATTGAAT ATGAGAAAGC CCAATCGTCC
    CAGGATGCTG TGTCTTCCAT GAACCTTTTT GACCTGGGGG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCCCCTAT GCCCCTGCTT ACACCGGCCA CACCTGGAGG CCTCCCGCCG
    GCTGCTGCCG TGGCCGCAGC TGCAGCCACA GCCAAGATTA CAGCTCAGGA AGCAGTGGCT
    GGAGCAGCAG TACTGGGTAC CCTAGCCACT CCTGGACTGG TGTCCCCTGC ACTGACTCTG
    GCCCAGCCCC TGGGGGCTCT ACCCCAGGCC GTCATGGCTG CCCAGGCCCC AGGAGTCATC
    ACAGGTGTGA CACCGGCTCG GCCTCCCATT CCGGTCACCA TCCCCTCTGT TGGTGTGGTG
    AATCCCATCC TGGCCAGCCC TCCGACACTT GGTCTCCTGG AGCCTAAGAA GGAGAAGGAG
    GAGGAGGAGC TGTTTCCCGA GTCGGAGCGG CCGGAGATGC TGAGTGAGCA GGAGCACATG
    AGCATCTCAG GAAGCAGCGC CCGCCACATG GTGATGCAGA AGCTCCTCCG CAAGCAGGAG
    TCCACAGTGA TGGTTCTGCG TAACATGGTG GACCCCAAGG ACATCGACGA TGACCTGGAA
    GGGGAGGTGA CTGAGGAGTG TGGCAAGTTT GGTGCTGTCA ACCGTGTCAT CATCTACCAA
    GAGAAGCAGG GCGAGGAAGA GGACGCGGAG ATCATTGTCA AGATTTTTGT GGAGTTTTCC
    GTAGCCTCTG AGACTCACAA GGCCATCCAG GCCCTCAATG GGCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA TGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239074
    Clone ID Related Accession (Same CDS sequence) NM_001046133.3
    Accession Version NM_001046133.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE251077.1, BC105447.1, DV852174.1 and NKLS02000014.1. On Jan 24, 2018 this sequence version replaced NM_001046133.2. ##Evidence-Data-START## Transcript exon combination :: SRR5571265.6606.1, SRR5571254.19233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCACTGGC CATCATGTGC
    CGGGTCTACG TGGGCTCCAT CTACTACGAG CTAGGGGAGG ACACCATCCG CCAGGCGTTC
    GCCCCCTTTG GGCCTATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CGTTTGTGGA GTATGAGGTC CCAGAAGCCG CCCAGCTGGC CTTGGAGCAG
    ATGAATTCCG TGATGCTGGG AGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCTCAGC CCATCATAGA CCAGCTGGCC GAGGAGGCGC GAGCCTTCAA CCGCATCTAC
    GTGGCTTCCG TGCACCAGGA CCTCTCGGAT GATGACATCA AGAGTGTGTT TGAGGCCTTT
    GGCAAGATCA AATCCTGCAC GCTGGCTCGG GACCCCACGA CTGGCAAACA CAAGGGCTAT
    GGCTTCATTG AATATGAGAA AGCCCAATCG TCCCAGGATG CTGTGTCTTC CATGAACCTT
    TTTGACCTGG GGGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCCCC TATGCCCCTG
    CTTACACCGG CCACACCTGG AGGCCTCCCG CCGGCTGCTG CCGTGGCCGC AGCTGCAGCC
    ACAGCCAAGA TTACAGCTCA GGAAGCAGTG GCTGGAGCAG CAGTACTGGG TACCCTAGCC
    ACTCCTGGAC TGGTGTCCCC TGCACTGACT CTGGCCCAGC CCCTGGGGGC TCTACCCCAG
    GCCGTCATGG CTGCCCAGGC CCCAGGAGTC ATCACAGGTG TGACACCGGC TCGGCCTCCC
    ATTCCGGTCA CCATCCCCTC TGTTGGTGTG GTGAATCCCA TCCTGGCCAG CCCTCCGACA
    CTTGGTCTCC TGGAGCCTAA GAAGGAGAAG GAGGAGGAGG AGCTGTTTCC CGAGTCGGAG
    CGGCCGGAGA TGCTGAGTGA GCAGGAGCAC ATGAGCATCT CAGGAAGCAG CGCCCGCCAC
    ATGGTGATGC AGAAGCTCCT CCGCAAGCAG GAGTCCACAG TGATGGTTCT GCGTAACATG
    GTGGACCCCA AGGACATCGA CGATGACCTG GAAGGGGAGG TGACTGAGGA GTGTGGCAAG
    TTTGGTGCTG TCAACCGTGT CATCATCTAC CAAGAGAAGC AGGGCGAGGA AGAGGACGCG
    GAGATCATTG TCAAGATTTT TGTGGAGTTT TCCGTAGCCT CTGAGACTCA CAAGGCCATC
    CAGGCCCTCA ATGGGCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTATGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039598.2
    CDS206..1798
    Translation

    Target ORF information:

    RefSeq Version NM_001046133.3
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001046133.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC GCTAGGGACA GACTCCATCA AGATGGAGAA CGGGCAGGGC 
    ACAGCCGCGA AGCTGGGACT GCCTCCCTTG ACGCCCGAGC AGCAGGAGGC CCTCCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGCGTGC TGGTGAAACA GACCATCGCC
    CACCAGCAGC AGCAGCTTAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCACTGGC CATCATGTGC
    CGGGTCTACG TGGGCTCCAT CTACTACGAG CTAGGGGAGG ACACCATCCG CCAGGCGTTC
    GCCCCCTTTG GGCCTATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CGTTTGTGGA GTATGAGGTC CCAGAAGCCG CCCAGCTGGC CTTGGAGCAG
    ATGAATTCCG TGATGCTGGG AGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCTCAGC CCATCATAGA CCAGCTGGCC GAGGAGGCGC GAGCCTTCAA CCGCATCTAC
    GTGGCTTCCG TGCACCAGGA CCTCTCGGAT GATGACATCA AGAGTGTGTT TGAGGCCTTT
    GGCAAGATCA AATCCTGCAC GCTGGCTCGG GACCCCACGA CTGGCAAACA CAAGGGCTAT
    GGCTTCATTG AATATGAGAA AGCCCAATCG TCCCAGGATG CTGTGTCTTC CATGAACCTT
    TTTGACCTGG GGGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCCCC TATGCCCCTG
    CTTACACCGG CCACACCTGG AGGCCTCCCG CCGGCTGCTG CCGTGGCCGC AGCTGCAGCC
    ACAGCCAAGA TTACAGCTCA GGAAGCAGTG GCTGGAGCAG CAGTACTGGG TACCCTAGCC
    ACTCCTGGAC TGGTGTCCCC TGCACTGACT CTGGCCCAGC CCCTGGGGGC TCTACCCCAG
    GCCGTCATGG CTGCCCAGGC CCCAGGAGTC ATCACAGGTG TGACACCGGC TCGGCCTCCC
    ATTCCGGTCA CCATCCCCTC TGTTGGTGTG GTGAATCCCA TCCTGGCCAG CCCTCCGACA
    CTTGGTCTCC TGGAGCCTAA GAAGGAGAAG GAGGAGGAGG AGCTGTTTCC CGAGTCGGAG
    CGGCCGGAGA TGCTGAGTGA GCAGGAGCAC ATGAGCATCT CAGGAAGCAG CGCCCGCCAC
    ATGGTGATGC AGAAGCTCCT CCGCAAGCAG GAGTCCACAG TGATGGTTCT GCGTAACATG
    GTGGACCCCA AGGACATCGA CGATGACCTG GAAGGGGAGG TGACTGAGGA GTGTGGCAAG
    TTTGGTGCTG TCAACCGTGT CATCATCTAC CAAGAGAAGC AGGGCGAGGA AGAGGACGCG
    GAGATCATTG TCAAGATTTT TGTGGAGTTT TCCGTAGCCT CTGAGACTCA CAAGGCCATC
    CAGGCCCTCA ATGGGCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTATGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222251
    Clone ID Related Accession (Same CDS sequence) XM_025001511.1
    Accession Version XM_025001511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product poly(U)-binding-splicing factor PUF60 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCACTGGCCA TCATGTGCCG GGTCTACGTG GGCTCCATCT ACTACGAGCT AGGGGAGGAC
    ACCATCCGCC AGGCGTTCGC CCCCTTTGGG CCTATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCG TTTGTGGAGT ATGAGGTCCC AGAAGCCGCC
    CAGCTGGCCT TGGAGCAGAT GAATTCCGTG ATGCTGGGAG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCTCAGCCC ATCATAGACC AGCTGGCCGA GGAGGCGCGA
    GCCTTCAACC GCATCTACGT GGCTTCCGTG CACCAGGACC TCTCGGATGA TGACATCAAG
    AGTGTGTTTG AGGCCTTTGG CAAGATCAAA TCCTGCACGC TGGCTCGGGA CCCCACGACT
    GGCAAACACA AGGGCTATGG CTTCATTGAA TATGAGAAAG CCCAATCGTC CCAGGATGCT
    GTGTCTTCCA TGAACCTTTT TGACCTGGGG GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCCCCTA TGCCCCTGCT TACACCGGCC ACACCTGGAG GCCTCCCGCC GGCTGCTGCC
    GTGGCCGCAG CTGCAGCCAC AGCCAAGATT ACAGCTCAGG AAGCAGTGGC TGGAGCAGCA
    GTACTGGGTA CCCTAGCCAC TCCTGGACTG GTGTCCCCTG CACTGACTCT GGCCCAGCCC
    CTGGGGGCTC TACCCCAGGC CGTCATGGCT GCCCAGGCCC CAGGAGTCAT CACAGGTGTG
    ACACCGGCTC GGCCTCCCAT TCCGGTCACC ATCCCCTCTG TTGGTGTGGT GAATCCCATC
    CTGGCCAGCC CTCCGACACT TGGTCTCCTG GAGCCTAAGA AGGAGAAGGA GGAGGAGGAG
    CTGTTTCCCG AGTCGGAGCG GCCGGAGATG CTGAGTGAGC AGGAGCACAT GAGCATCTCA
    GGAAGCAGCG CCCGCCACAT GGTGATGCAG AAGCTCCTCC GCAAGCAGGA GTCCACAGTG
    ATGGTTCTGC GTAACATGGT GGACCCCAAG GACATCGACG ATGACCTGGA AGGGGAGGTG
    ACTGAGGAGT GTGGCAAGTT TGGTGCTGTC AACCGTGTCA TCATCTACCA AGAGAAGCAG
    GGCGAGGAAG AGGACGCGGA GATCATTGTC AAGATTTTTG TGGAGTTTTC CGTAGCCTCT
    GAGACTCACA AGGCCATCCA GGCCCTCAAT GGGCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ATGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857279.1
    CDS3235..4785
    Translation

    Target ORF information:

    RefSeq Version XM_025001511.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(U) binding splicing factor 60 (PUF60), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGGGCAC AGCCGCGAAG CTGGGACTGC CTCCCTTGAC GCCCGAGCAG 
    CAGGAGGCCC TCCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGCGTGCTG
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTTACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCACTGGCCA TCATGTGCCG GGTCTACGTG GGCTCCATCT ACTACGAGCT AGGGGAGGAC
    ACCATCCGCC AGGCGTTCGC CCCCTTTGGG CCTATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCG TTTGTGGAGT ATGAGGTCCC AGAAGCCGCC
    CAGCTGGCCT TGGAGCAGAT GAATTCCGTG ATGCTGGGAG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCTCAGCCC ATCATAGACC AGCTGGCCGA GGAGGCGCGA
    GCCTTCAACC GCATCTACGT GGCTTCCGTG CACCAGGACC TCTCGGATGA TGACATCAAG
    AGTGTGTTTG AGGCCTTTGG CAAGATCAAA TCCTGCACGC TGGCTCGGGA CCCCACGACT
    GGCAAACACA AGGGCTATGG CTTCATTGAA TATGAGAAAG CCCAATCGTC CCAGGATGCT
    GTGTCTTCCA TGAACCTTTT TGACCTGGGG GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCCCCTA TGCCCCTGCT TACACCGGCC ACACCTGGAG GCCTCCCGCC GGCTGCTGCC
    GTGGCCGCAG CTGCAGCCAC AGCCAAGATT ACAGCTCAGG AAGCAGTGGC TGGAGCAGCA
    GTACTGGGTA CCCTAGCCAC TCCTGGACTG GTGTCCCCTG CACTGACTCT GGCCCAGCCC
    CTGGGGGCTC TACCCCAGGC CGTCATGGCT GCCCAGGCCC CAGGAGTCAT CACAGGTGTG
    ACACCGGCTC GGCCTCCCAT TCCGGTCACC ATCCCCTCTG TTGGTGTGGT GAATCCCATC
    CTGGCCAGCC CTCCGACACT TGGTCTCCTG GAGCCTAAGA AGGAGAAGGA GGAGGAGGAG
    CTGTTTCCCG AGTCGGAGCG GCCGGAGATG CTGAGTGAGC AGGAGCACAT GAGCATCTCA
    GGAAGCAGCG CCCGCCACAT GGTGATGCAG AAGCTCCTCC GCAAGCAGGA GTCCACAGTG
    ATGGTTCTGC GTAACATGGT GGACCCCAAG GACATCGACG ATGACCTGGA AGGGGAGGTG
    ACTGAGGAGT GTGGCAAGTT TGGTGCTGTC AACCGTGTCA TCATCTACCA AGAGAAGCAG
    GGCGAGGAAG AGGACGCGGA GATCATTGTC AAGATTTTTG TGGAGTTTTC CGTAGCCTCT
    GAGACTCACA AGGCCATCCA GGCCCTCAAT GGGCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ATGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.