UBE3B cDNA ORF clone, Bos taurus(cattle)

The following UBE3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01649 NM_001206232.1
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 NM_001206232.3
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_005217809.4
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_005217808.4
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_010813910.3
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_024977190.1
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_024977188.1
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_010813908.3
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_024977189.1
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_024977192.1
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_015475464.2
Latest version!
Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa27852 XM_005217808.3 Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 NM_001206232.2 Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_010813910.2 Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_010813908.2 Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_005217809.3 Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27852 XM_015475464.1 Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01649
    Clone ID Related Accession (Same CDS sequence) NM_001206232.1
    Accession Version NM_001206232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product ubiquitin-protein ligase E3B
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03123071.1. On Apr 20, 2011 this sequence version replaced XM_867990.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GATAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193161.1
    CDS1..3207
    Translation

    Target ORF information:

    RefSeq Version NM_001206232.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GATAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version NM_001206232.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02045478.1. On Jun 30, 2017 this sequence version replaced NM_001206232.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193161.1
    CDS1..3207
    Translation

    Target ORF information:

    RefSeq Version NM_001206232.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version NM_001206232.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On May 29, 2018 this sequence version replaced NM_001206232.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721694.3522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193161.1
    CDS1..3207
    Translation

    Target ORF information:

    RefSeq Version NM_001206232.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206232.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_005217809.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217866.1
    CDS647..3853
    Translation

    Target ORF information:

    RefSeq Version XM_005217809.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_005217808.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217808.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217865.1
    CDS357..3563
    Translation

    Target ORF information:

    RefSeq Version XM_005217808.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217808.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_024977188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832956.1
    CDS362..3568
    Translation

    Target ORF information:

    RefSeq Version XM_024977188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_024977189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832957.1
    CDS283..3489
    Translation

    Target ORF information:

    RefSeq Version XM_024977189.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_005217808.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217865.1
    CDS492..3698
    Translation

    Target ORF information:

    RefSeq Version XM_005217808.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217808.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_010813910.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812212.1
    CDS429..3635
    Translation

    Target ORF information:

    RefSeq Version XM_010813910.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_010813910.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812212.1
    CDS284..3490
    Translation

    Target ORF information:

    RefSeq Version XM_010813910.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813910.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_015475464.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330950.1
    CDS636..3842
    Translation

    Target ORF information:

    RefSeq Version XM_015475464.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_024977192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832960.1
    CDS288..3494
    Translation

    Target ORF information:

    RefSeq Version XM_024977192.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_010813908.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812210.1
    CDS503..3709
    Translation

    Target ORF information:

    RefSeq Version XM_010813908.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813908.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_024977190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832958.1
    CDS428..3634
    Translation

    Target ORF information:

    RefSeq Version XM_024977190.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_010813908.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812210.1
    CDS358..3564
    Translation

    Target ORF information:

    RefSeq Version XM_010813908.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813908.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_005217809.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217866.1
    CDS502..3708
    Translation

    Target ORF information:

    RefSeq Version XM_005217809.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217809.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27852
    Clone ID Related Accession (Same CDS sequence) XM_005217809.3 , XM_005217808.4 , XM_024977188.1 , XM_024977189.1 , XM_005217808.3 , XM_010813910.2 , NM_001206232.2 , NM_001206232.3 , XM_010813910.3 , XM_015475464.1 , XM_024977192.1 , XM_010813908.2 , XM_024977190.1 , XM_010813908.3 , XM_005217809.4 , XM_015475464.2
    Accession Version XM_015475464.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330950.1
    CDS494..3700
    Translation

    Target ORF information:

    RefSeq Version XM_015475464.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCACCG TTTCTCAGAC CTCAAGAGCT TGGTTCATCG ACAGAGCCCG TCAGGCTCGA 
    GAGGAAAGGC TGGTGCAGAA GGAACGAGAA CGGGCGGCTG TGGAGATTCA GGCCCACATA
    CGGAGCTTTC TCTGTCGGCG TCGGCTGCAA AGAGAAATCA GGAGAGAGAT TGATGAGTTT
    TTTAAAGCAG AAGACTCTGG GTCCAGTAAA AGAAGCGCAC TTTGTATTTT TAAGATTGCC
    AGGAAACTGC TGTTCTTGTT CAGAATTAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT
    CGCTGTATCC TGAGCAGCAT GGAAGCTGAG AATGAACCCA AGGTGTGGTA CGTGTCCCTG
    GCTCTTTCCA AGGATCTCAC TCTCCTATGG ATTAAACAGA TCAAAGACAT CCTGTGGTAT
    TGCTGTGAGT TTCTTGAGCA GCTCAAGCCG GAAATTTTGC AAGACTCCAA ACTCATCACC
    CTGTACCTCA CGATGCTTGT CACCTTCACG GACACGTCAA CGTGGAAAAT TCTCCGGGGA
    AAAGGTGAGA GTCTTCGACC AGCCCTGAAC CACATCTGTG CAAACATAAT GGGCCATCTC
    AACCAGCGTG GATTCTACCC TGTTCTGCAG ATCCTGCTAA CCCGTGGCCT GGCGAGACCC
    CGGCCTTGTC TATCCAAAGG CACTTTAACG GCCGCCTTTT CCCTAGCGAT GCGCCCCGTG
    CTTGCTGCAC AATTTTCAGA CAATCTAATC CGGCCGTTCC TCATCCACAT CGTGTCCGTG
    CCCGCCCTCG TGACTCATCT CAGCACTGTG ACCCCCGAGC GCCTCACTGT TTTAGAATCT
    CATGACATGC TCCGTAAATT CATCGCATTT TTAAGAGATG AAGATCGATG CCGTGATGTG
    TGTGAAAGCT TGGAAGGATG CCATACGCTT TGTCTCATGG GCAACCTCCT ACAGTTGGGC
    TCCCTCAGCC CCAGGGTGTT AGAGGAGGAG ACAGACGGCT TCGTGAGTCT GCTCACCCAG
    ATGCTCTGCT ACTGTCAGAA GTACGTGTCC CAGAAGAAGT CCAACCTGAC CCACTGGCAC
    CCCGTCCTCG GCTGGTTCTC CCAGTCCGTG GACTACGGCC TCAATGAGTC CATGCCCTTG
    ATCACCAAGC AGCTGCAGTT CCTGTGGGGG GTGCCTCTTA TCCGGGTCTT CTTCAGCGAC
    ATCCTGAGCA AGAAGCTGCT GGAGACCCAG GAGCCGGCCC ACGCGCCGCC GGCCTCCCCG
    CAGAACGTGC TGCCCGTGAA GACTCTCCTA AAGCGAGCGT TTCAGAAGTC AGCGTCAGTC
    CGCAACATAC TGAGGCCCGT CGGGGGCAAG CGTGTGGACT CCACGGAGGC GCAGAAGGTC
    TGCAGGGTCT GCGTCCTCTA CCAGACTTCG CTGGCGACTC TCACCCAGAT CCGGCTGCAG
    GTCCTCACAG GTCTCACTTA CCTCGATGAC CTGCTGCCCA AACTGTGGGC ATTTATCTGT
    GAGCTGGGGC CCCACGGAGG GCTGAAGCTC TTCTTGGAGT GCCTCAACAA TGACACGGAG
    GAGTCTAAGC AGCTTCTGGC CATGCTGATG CTGTTCTGTG ACTGTTCCCG GCACCTCATC
    ACGATCCTCG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG
    GTCACCATCT CCTCTTTTCT GAACTCCTTT GTGTTTAAGA TGATCTGGGA TGGAATCGTA
    GAGAACGCCA AGGGCGAGAC CCTGGAGTTG TTCCAGTCCG TCCATGGGTG GCTGATGGTG
    CTGTACGAGC GGGACTGCCG GCGGCGCTTC GCACCTGAGG ACCACTGGCT GCGAAAGGAT
    CTCAAACCCA GCGTGCTCTT CCAGGAACTT GACAAGGACA GAAAGCGGGC CCAGCTGATC
    CTGCAGTACA TCCCACACGT CATTCCTCAC AAAAACAGAG TTCTCCTGTT CCGAAACATG
    GTGACCAAGG AGAAGGAGAA ACTGGGGCTT GTGGAAACCA GCTCGGCCTC CCCTCACGTC
    ACCCACATCA CCATCCGCCG GTCCCGGATG CTGGAGGACG GCTACGAGCA GCTCCGGCAG
    CTCTCGCAGC ACGCCATGAA GGGCGTCATC CGCGTCAAGT TTGTCAACGA CCTCGGGGTG
    GACGAGGCCG GGATTGATCA AGACGGTGTT TTCAAGGAGT TCTTGGAAGA GATAATCAAG
    AGAGTGTTCG ACCCAGCGCT CAATCTATTC AAGACGACCA GTGGAGACGA GAGACTCTAC
    CCTTCGCCCA CGTCCTACAT CCATGAGAAT TACCTACAGC TCTTTGAGTT TGTGGGCAAG
    ATGCTGGGGA AGGCGGTGTA CGAGGGAATC GTGGTCGACG TGCCCTTCGC GTCCTTCTTC
    CTGAGCCAGC TGCTCGGGCA CCACCACAGC GTCTTCTACA GCTCTGTGGA TGAGCTGCCC
    TCTCTGGACT CAGAGTTCTA CAAAAACCTC ACATCCATCA AGCGGTACGA TGGGGACATC
    GCTGACTTGG GCCTGACGCT GTCGTATGAT GAGGATGTCA TGGGTCAGCT TGTTTGCCAT
    GAACTGGTTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC
    CATCTGATGG CCCACTTTCG AATGCACACG CAAATCAAGA ACCAGACAGC TGCCCTCATT
    AGCGGCTTCC GTTCCATCAT CAAGCCTGAG TGGATCCGGA TGTTCTCGAC TCCTGAGCTG
    CAGCGACTCA TCTCTGGGGA CAATGCTGAG ATCGATCTGG AAGATCTGAA GAAGCACACG
    GTGTACTACG GAGGTTTCCA TGGGAGCCAC AGGGTCATCA TCTGGCTCTG GGATATCCTT
    GCCTCTGACT TCACGCCCGA AGAGAGAGCC ATGTTTCTGA AGTTTGTGAC GAGCTGTTCC
    CGGCCCCCAC TCCTGGGATT CGCCTACCTC AAGCCTCCTT TCTCCATCCG CTGTGTTGAA
    GTGTCGGATG ATCAGGACAC GGGGGACACC CTGGGCAGCG TCCTCCGGGG CTTCTTCACC
    ATCCGCAAGC GGGAGCCGGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC
    AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTGCGGGAGA AGCTACGCTA TGCCATCAGC
    ATGAACACGG GCTTCGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.