NLRC4 cDNA ORF clone, Bos taurus(cattle)

The following NLRC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa48320 XM_010809867.2
Latest version!
Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48321 XM_010809868.2
Latest version!
Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa220267 XM_010809868.3
Latest version!
Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220266 XM_005212521.4
Latest version!
Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa01327 NM_001192323.2
Latest version!
Bos taurus NLR family CARD domain containing 4 (NLRC4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa01327 XM_005212521.3
Latest version!
Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa48320
    Clone ID Related Accession (Same CDS sequence) XM_010809867.2
    Accession Version XM_010809867.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TCAGAATCTT
    CATAACCTGG CCAGACTGAG CATTCTTGAT TTATCTGAAA ATCATCTGGA AAAGGATGGA
    AAAGAAGCTC TTCAGCAACT AATTGACAGG CTGCATATCT TAGAACAGCT AACCGTTCTG
    ATGCTGCCCT GGTGTGGTGA TGTACGAGTC AGTCTGGCCA GACTGTTGGA GCAACTGGAG
    AGGGTCCCAC AGCTCGTCAA GCTTGGGCTG AAAAACTGGA GACTCACAGA TGCAGAGATA
    AGAATTCTAG GTGTATTTTT TGAAAAGAAT CCTCTGGAAA ACTTCCAGCA GTTGGATTTG
    GCAGGAAATT GTGTAAGCAG CGATGGATGG CTTGCCTTCA TGAGTGGATT TGAGAATCTT
    AAGGAACTAG TGTTTTTTGA CTTCAGTACG AAAGGACTCT TACCTGATGC ATCATTAGTC
    AGGAAACTTA GCCACGTGTT ATCCAAACTA ACTTTTCTGC AAGAGGTTCA GCTTGTTGGG
    TGGCAACTTG ATGACGATGA TGTCAGTGTT CTTAAAGGTG CTTTTAAACT AGTAATTGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808169.1
    CDS517..3399
    Translation

    Target ORF information:

    RefSeq Version XM_010809867.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TCAGAATCTT
    CATAACCTGG CCAGACTGAG CATTCTTGAT TTATCTGAAA ATCATCTGGA AAAGGATGGA
    AAAGAAGCTC TTCAGCAACT AATTGACAGG CTGCATATCT TAGAACAGCT AACCGTTCTG
    ATGCTGCCCT GGTGTGGTGA TGTACGAGTC AGTCTGGCCA GACTGTTGGA GCAACTGGAG
    AGGGTCCCAC AGCTCGTCAA GCTTGGGCTG AAAAACTGGA GACTCACAGA TGCAGAGATA
    AGAATTCTAG GTGTATTTTT TGAAAAGAAT CCTCTGGAAA ACTTCCAGCA GTTGGATTTG
    GCAGGAAATT GTGTAAGCAG CGATGGATGG CTTGCCTTCA TGAGTGGATT TGAGAATCTT
    AAGGAACTAG TGTTTTTTGA CTTCAGTACG AAAGGACTCT TACCTGATGC ATCATTAGTC
    AGGAAACTTA GCCACGTGTT ATCCAAACTA ACTTTTCTGC AAGAGGTTCA GCTTGTTGGG
    TGGCAACTTG ATGACGATGA TGTCAGTGTT CTTAAAGGTG CTTTTAAACT AGTAATTGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48321
    Clone ID Related Accession (Same CDS sequence) XM_010809868.2
    Accession Version XM_010809868.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA
    TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG
    CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT
    CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA
    AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT
    CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT
    GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA
    GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT
    TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT
    AAAGGTGCTT TTAAACTAGT AATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808170.1
    CDS517..3306
    Translation

    Target ORF information:

    RefSeq Version XM_010809868.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809868.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA
    TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG
    CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT
    CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA
    AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT
    CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT
    GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA
    GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT
    TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT
    AAAGGTGCTT TTAAACTAGT AATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220267
    Clone ID Related Accession (Same CDS sequence) XM_010809868.3
    Accession Version XM_010809868.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA
    TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG
    CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT
    CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA
    AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT
    CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT
    GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA
    GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT
    TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT
    AAAGGTGCTT TTAAACTAGT AATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808170.2
    CDS398..3187
    Translation

    Target ORF information:

    RefSeq Version XM_010809868.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809868.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA
    TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG
    CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT
    CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA
    AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT
    CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT
    GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA
    GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT
    TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT
    AAAGGTGCTT TTAAACTAGT AATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220266
    Clone ID Related Accession (Same CDS sequence) XM_005212521.4
    Accession Version XM_005212521.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212578.2
    CDS398..3451
    Translation

    Target ORF information:

    RefSeq Version XM_005212521.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212521.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01327
    Clone ID Related Accession (Same CDS sequence) NM_001192323.2 , XM_005212521.3
    Accession Version NM_001192323.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02030442.1. On Apr 15, 2011 this sequence version replaced NM_001192323.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179252.2
    CDS20..3073
    Translation

    Target ORF information:

    RefSeq Version NM_001192323.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 4 (NLRC4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01327
    Clone ID Related Accession (Same CDS sequence) NM_001192323.2 , XM_005212521.3
    Accession Version XM_005212521.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NLR family CARD domain-containing protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212578.1
    CDS507..3560
    Translation

    Target ORF information:

    RefSeq Version XM_005212521.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212521.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
    AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC
    ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG
    AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG
    TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT
    TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG
    GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG
    AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT
    CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG
    CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC
    TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC
    CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG
    CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA
    ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC
    ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG
    ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC
    ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG
    CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC
    CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA
    TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG
    TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT
    TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA
    GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA
    CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC
    ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC
    TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC
    GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA
    GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT
    GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA
    GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG
    GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT
    ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC
    CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA
    GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG
    ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG
    CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG
    ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA
    ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA
    GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG
    GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT
    CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC
    TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA
    CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA
    GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT
    TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA
    GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT
    AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT
    GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.