DGKZ gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024975187.1	XP_024830955.1	diacylglycerol kinase zeta isoform X1
XM_024975190.1	XP_024830958.1	diacylglycerol kinase zeta isoform X4
XM_024975193.1	XP_024830961.1	diacylglycerol kinase zeta isoform X6
XM_024975189.1	XP_024830957.1	diacylglycerol kinase zeta isoform X3
XM_024975196.1	XP_024830964.1	diacylglycerol kinase zeta isoform X9
XM_024975197.1	XP_024830965.1	diacylglycerol kinase zeta isoform X10
XM_024975199.1	XP_024830967.1	diacylglycerol kinase zeta isoform X12
XM_024975188.1	XP_024830956.1	diacylglycerol kinase zeta isoform X2
XM_024975191.1	XP_024830959.1	diacylglycerol kinase zeta isoform X4
XM_024975194.1	XP_024830962.1	diacylglycerol kinase zeta isoform X7
XM_024975198.1	XP_024830966.1	diacylglycerol kinase zeta isoform X11
XM_024975195.1	XP_024830963.1	diacylglycerol kinase zeta isoform X8
XM_024975192.1	XP_024830960.1	diacylglycerol kinase zeta isoform X5
NM_001278652.1	NP_001265581.1	diacylglycerol kinase zeta

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36369	NM_001278652.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa222724	XM_024975192.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222725	XM_024975193.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222726	XM_024975194.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222727	XM_024975195.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222728	XM_024975196.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222729	XM_024975197.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222730	XM_024975198.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222731	XM_024975199.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa222720	XM_024975187.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222721	XM_024975188.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222722	XM_024975189.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222723	XM_024975191.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222723	XM_024975190.1 Latest version!	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

DGKZ ( NM_001278652.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001265581.1 Bos taurus diacylglycerol kinase zeta (DGKZ), mRNA.

Price & Availability↑

CloneID	OBa36369
Clone ID Related Accession (Same CDS sequence)	NM_001278652.1
Accession Version	NM_001278652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151801.1, DV910887.1, DV820601.1, EE348312.1, EH208627.1 and BI536253.1. On or before Jul 7, 2013 this sequence version replaced XM_002706705.1, XM_002701404.1, XM_002693565.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT GCAGCCTGCA AGATTGTGGT CCACACACCC 
TGCATTGAGC AGCTCGAGAA GATAAATTTC CGCTGTAAGC CATCCTTCCG TGAATCGGGC 
TCCAGGAACG TCCGTGAGCC AACCTTCGTG CGGCACCACT GGGTACACCG GCGACGCCAG 
GACGGCAAGT GTCGGCACTG CGGGAAGGGC TTCCAGCAGA AGTTCACCTT CCACAGCAAG 
GAGATCGTGG CCATCAGCTG CTCCTGGTGC AAGCAAGCAT ACCACAGCAA GGTGTCCTGC 
TTCATGCTGC AGCAGATCGA GGAGCCGTGC TCCCTGGGGG TCCACGCCGC TGTGGTCATC 
CCCCCCACCT GGATCCTCCG GGCCCGCAGG CCCCAGAACA CCCTCAAAGC CAGCAAGAAG 
AAAAAAAGAG CATCCTTCAA GAGGAAGTCT AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG 
AGACCCTTCA TCATCAGGCC TACCCCGTCC CCCCTCATGA AGCCCCTGCT GGTGTTCGTG 
AACCCCAAGA GTGGGGGCAA CCAGGGCGCC AAGATCATCC AGTCCTTCCT CTGGTATCTG 
AATCCCCGGC AAGTCTTTGA CCTGAGCCAG GGGGGCCCCA GGGAGGCGCT GGAGATGTAT 
CGCCGAGTGC ACAACCTGCG GATCCTGGCC TGCGGGGGTG ACGGCACGGT CGGCTGGATC 
CTCTCCACGC TGGACCAGCT GCGCTTGAAG CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG 
GGCACTGGCA ATGACTTGGC CCGCACCCTC AACTGGGGCG GGGGCTACAC TGACGAGCCT 
GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG GGCAACGTGG TACAGCTGGA CCGCTGGGAC 
CTCCATGCGG AGCCCAACCC CGAGGCGGGG CCCGAGGAGC GAGATGAGGG GGCCACCGAC 
CAGCTGCCTC TGGATGTCTT CAACAACTAC TTCAGCCTGG GCTTTGACGC CCACGTCACC 
CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC CCGGAGAAGT TCAACAGCCG CTTCCGGAAT 
AAGATGTTCT ACGCCGGGAC AGCCTTCTCT GACTTCCTGA TGGGCAGCTC CAAGGACTTG 
GCCAAGCACA TCCGCGTGGT GTGTGATGGG ACTGACCTGA CCCCCAAGAT TCAGGACCTG 
AAACCCCAGT GCATTGTTTT CTTGAACATC CCCAGGTACT GCGCGGGCAC CATGCCCTGG 
GGCCACCCTG GGGAGCACCA TGACTTTGAG CCCCAGCGGC ACGACGATGG CTACCTCGAG 
GTCATCGGCT TTACCATGAC CTCCCTGGCT GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG 
CTGACGCAGT GCCGAGAGGT GCTGCTCACC ACGTCCAAAG CCATCCCGGT GCAGGTGGAC 
GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC ATCCGCATTG CCCTGCGCAA CCAGGCCACC 
ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC GCCCCCCTGC ACAGCGACCA GCAGCCGGTG 
CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG GTCAGCATGC ACGACTACGA GGCCCTGCAC 
TACGACAAGG AGCAGCTCAA AGAGGCTTCT GTGCCGCTGG GCACTGTGGT GGTCCCAGGA 
GACAGCGACC TGGAGCTGTG CCGCGCTCAC ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT 
GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA CTGTCCCCCA AGTGGTGCTT CCTCGATGCC 
ACCACTGCCA GCCGCTTCTA CAGAATCGAC AGGGCCCAGG AACACCTCAA CTACGTGACC 
GAGATCGCAC AGGACGAGAT TTATATCCTG GACCCTGAGC TGCTGGGGGC ATCTGCCCGT 
CCTGACCTCC CCACCCCCAC GTCCCCTCTC CCCACCTCGC CCTGCTCCCC CACATCCCGG 
TCACTGCCAG GGGACGCTGC GCCCCCTACA GGTGAAGAGC TCATCGAGGC TGCAAAGAGG 
AACGATTTCT GTAAGCTCCA GGAGCTGCAC CGAGCTGGGG GTGACCTTAT GCACCGTGAT 
GAGCGGAGCC GCACGCTCCT GCACCACGCG GTCAGCACCG GCAGCAAGGA AGTGGTCCGC 
TACCTGCTGG AGCACGCGCC CACTGAGATC CTTGATGCCG TGGAGGAAAA CGGGGAGACC 
TGCCTGCACC AGGCGGCAGC CCTGGGCCAG CGCACCATCT GCCACTACAT CGTGGAGGCC 
GGGGCCTCGC TCATGAAGAC TGACCAGCAG GGTGACACTC CCCGGCAGAG AGCGGAGAAG 
GCTCAGGACA CGGAACTGGC GGCGTACCTG GAAAACCGGC AGCATTACCA GATGATCCAG 
CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265581.1
CDS	118..2904
Translation	MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLSKRRFPGLRLFGHRKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	NM_001278652.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278652.1

ORF Insert Sequence:

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT GCAGCCTGCA AGATTGTGGT CCACACACCC 
TGCATTGAGC AGCTCGAGAA GATAAATTTC CGCTGTAAGC CATCCTTCCG TGAATCGGGC 
TCCAGGAACG TCCGTGAGCC AACCTTCGTG CGGCACCACT GGGTACACCG GCGACGCCAG 
GACGGCAAGT GTCGGCACTG CGGGAAGGGC TTCCAGCAGA AGTTCACCTT CCACAGCAAG 
GAGATCGTGG CCATCAGCTG CTCCTGGTGC AAGCAAGCAT ACCACAGCAA GGTGTCCTGC 
TTCATGCTGC AGCAGATCGA GGAGCCGTGC TCCCTGGGGG TCCACGCCGC TGTGGTCATC 
CCCCCCACCT GGATCCTCCG GGCCCGCAGG CCCCAGAACA CCCTCAAAGC CAGCAAGAAG 
AAAAAAAGAG CATCCTTCAA GAGGAAGTCT AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG 
AGACCCTTCA TCATCAGGCC TACCCCGTCC CCCCTCATGA AGCCCCTGCT GGTGTTCGTG 
AACCCCAAGA GTGGGGGCAA CCAGGGCGCC AAGATCATCC AGTCCTTCCT CTGGTATCTG 
AATCCCCGGC AAGTCTTTGA CCTGAGCCAG GGGGGCCCCA GGGAGGCGCT GGAGATGTAT 
CGCCGAGTGC ACAACCTGCG GATCCTGGCC TGCGGGGGTG ACGGCACGGT CGGCTGGATC 
CTCTCCACGC TGGACCAGCT GCGCTTGAAG CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG 
GGCACTGGCA ATGACTTGGC CCGCACCCTC AACTGGGGCG GGGGCTACAC TGACGAGCCT 
GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG GGCAACGTGG TACAGCTGGA CCGCTGGGAC 
CTCCATGCGG AGCCCAACCC CGAGGCGGGG CCCGAGGAGC GAGATGAGGG GGCCACCGAC 
CAGCTGCCTC TGGATGTCTT CAACAACTAC TTCAGCCTGG GCTTTGACGC CCACGTCACC 
CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC CCGGAGAAGT TCAACAGCCG CTTCCGGAAT 
AAGATGTTCT ACGCCGGGAC AGCCTTCTCT GACTTCCTGA TGGGCAGCTC CAAGGACTTG 
GCCAAGCACA TCCGCGTGGT GTGTGATGGG ACTGACCTGA CCCCCAAGAT TCAGGACCTG 
AAACCCCAGT GCATTGTTTT CTTGAACATC CCCAGGTACT GCGCGGGCAC CATGCCCTGG 
GGCCACCCTG GGGAGCACCA TGACTTTGAG CCCCAGCGGC ACGACGATGG CTACCTCGAG 
GTCATCGGCT TTACCATGAC CTCCCTGGCT GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG 
CTGACGCAGT GCCGAGAGGT GCTGCTCACC ACGTCCAAAG CCATCCCGGT GCAGGTGGAC 
GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC ATCCGCATTG CCCTGCGCAA CCAGGCCACC 
ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC GCCCCCCTGC ACAGCGACCA GCAGCCGGTG 
CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG GTCAGCATGC ACGACTACGA GGCCCTGCAC 
TACGACAAGG AGCAGCTCAA AGAGGCTTCT GTGCCGCTGG GCACTGTGGT GGTCCCAGGA 
GACAGCGACC TGGAGCTGTG CCGCGCTCAC ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT 
GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA CTGTCCCCCA AGTGGTGCTT CCTCGATGCC 
ACCACTGCCA GCCGCTTCTA CAGAATCGAC AGGGCCCAGG AACACCTCAA CTACGTGACC 
GAGATCGCAC AGGACGAGAT TTATATCCTG GACCCTGAGC TGCTGGGGGC ATCTGCCCGT 
CCTGACCTCC CCACCCCCAC GTCCCCTCTC CCCACCTCGC CCTGCTCCCC CACATCCCGG 
TCACTGCCAG GGGACGCTGC GCCCCCTACA GGTGAAGAGC TCATCGAGGC TGCAAAGAGG 
AACGATTTCT GTAAGCTCCA GGAGCTGCAC CGAGCTGGGG GTGACCTTAT GCACCGTGAT 
GAGCGGAGCC GCACGCTCCT GCACCACGCG GTCAGCACCG GCAGCAAGGA AGTGGTCCGC 
TACCTGCTGG AGCACGCGCC CACTGAGATC CTTGATGCCG TGGAGGAAAA CGGGGAGACC 
TGCCTGCACC AGGCGGCAGC CCTGGGCCAG CGCACCATCT GCCACTACAT CGTGGAGGCC 
GGGGCCTCGC TCATGAAGAC TGACCAGCAG GGTGACACTC CCCGGCAGAG AGCGGAGAAG 
GCTCAGGACA CGGAACTGGC GGCGTACCTG GAAAACCGGC AGCATTACCA GATGATCCAG 
CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975192.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830960.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa222724
Clone ID Related Accession (Same CDS sequence)	XM_024975192.1
Accession Version	XM_024975192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTG AACCTCTCTC ATGTCAAAGC CTCTGGACTA GGTCGTTTAA GCCCTGTTTT 
GCAGAGGAGA AAATTGAGAC TCCAAAAGGT CAAGGTGCAG TTCACGGGCT CCCTCAGCGT 
CCAGACGAAG GTTTTCAGAC ATCGTCCAGC ACTGTCGTAC ATGGGTTGTC CATCCTGGCC 
TCTTCAGAAA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGCAGA AATCAGTGTC CCGGAGAAAG 
TGTGCAGCCT GCAAGATTGT GGTCCACACA CCCTGCATTG AGCAGCTCGA GAAGATAAAT 
TTCCGCTGTA AGCCATCCTT CCGTGAATCG GGCTCCAGGA ACGTCCGTGA GCCAACCTTC 
GTGCGGCACC ACTGGGTACA CCGGCGACGC CAGGACGGCA AGTGTCGGCA CTGCGGGAAG 
GGCTTCCAGC AGAAGTTCAC CTTCCACAGC AAGGAGATCG TGGCCATCAG CTGCTCCTGG 
TGCAAGCAAG CATACCACAG CAAGGTGTCC TGCTTCATGC TGCAGCAGAT CGAGGAGCCG 
TGCTCCCTGG GGGTCCACGC CGCTGTGGTC ATCCCCCCCA CCTGGATCCT CCGGGCCCGC 
AGGCCCCAGA ACACCCTCAA AGCCAGCAAG AAGAAAAAAA GAGCATCCTT CAAGAGGAAG 
TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC TGGAGACCCT TCATCATCAG GCCTACCCCG 
TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC GTGAACCCCA AGAGTGGGGG CAACCAGGGC 
GCCAAGATCA TCCAGTCCTT CCTCTGGTAT CTGAATCCCC GGCAAGTCTT TGACCTGAGC 
CAGGGGGGCC CCAGGGAGGC GCTGGAGATG TATCGCCGAG TGCACAACCT GCGGATCCTG 
GCCTGCGGGG GTGACGGCAC GGTCGGCTGG ATCCTCTCCA CGCTGGACCA GCTGCGCTTG 
AAGCCGCCGC CGCCAGTCGC CATCCTGCCC CTGGGCACTG GCAATGACTT GGCCCGCACC 
CTCAACTGGG GCGGGGGCTA CACTGACGAG CCTGTGTCCA AGATCCTGTC CCACGTGGAG 
GAGGGCAACG TGGTACAGCT GGACCGCTGG GACCTCCATG CGGAGCCCAA CCCCGAGGCG 
GGGCCCGAGG AGCGAGATGA GGGGGCCACC GACCAGCTGC CTCTGGATGT CTTCAACAAC 
TACTTCAGCC TGGGCTTTGA CGCCCACGTC ACCCTGGAGT TCCACGAGTC TCGAGAGGCC 
AACCCGGAGA AGTTCAACAG CCGCTTCCGG AATAAGATGT TCTACGCCGG GACAGCCTTC 
TCTGACTTCC TGATGGGCAG CTCCAAGGAC TTGGCCAAGC ACATCCGCGT GGTGTGTGAT 
GGGACTGACC TGACCCCCAA GATTCAGGAC CTGAAACCCC AGTGCATTGT TTTCTTGAAC 
ATCCCCAGGT ACTGCGCGGG CACCATGCCC TGGGGCCACC CTGGGGAGCA CCATGACTTT 
GAGCCCCAGC GGCACGACGA TGGCTACCTC GAGGTCATCG GCTTTACCAT GACCTCCCTG 
GCTGCGCTGC AGGTGGGCGG GCACGGCGAG CGGCTGACGC AGTGCCGAGA GGTGCTGCTC 
ACCACGTCCA AAGCCATCCC GGTGCAGGTG GACGGTGAGC CCTGCAAGCT CGCAGCCTCG 
CGCATCCGCA TTGCCCTGCG CAACCAGGCC ACCATGGTGC AGAAGGCCAA GCGGCGGAGC 
GCCGCCCCCC TGCACAGCGA CCAGCAGCCG GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC 
AGGGTCAGCA TGCACGACTA CGAGGCCCTG CACTACGACA AGGAGCAGCT CAAAGAGGCT 
TCTGTGCCGC TGGGCACTGT GGTGGTCCCA GGAGACAGCG ACCTGGAGCT GTGCCGCGCT 
CACATCGAGA GGCTCCGGCA GGAGCCCGAA GGTGCTGGAG CCAAGTCCCC GATGTGCCAG 
AAACTGTCCC CCAAGTGGTG CTTCCTCGAT GCCACCACTG CCAGCCGCTT CTACAGAATC 
GACAGGGCCC AGGAACACCT CAACTACGTG ACCGAGATCG CACAGGACGA GATTTATATC 
CTGGACCCTG AGCTGCTGGG GGCATCTGCC CGTCCTGACC TCCCCACCCC CACGTCCCCT 
CTCCCCACCT CGCCCTGCTC CCCCACATCC CGGTCACTGC CAGGGGACGC TGCGCCCCCT 
ACAGGTGAAG AGCTCATCGA GGCTGCAAAG AGGAACGATT TCTGTAAGCT CCAGGAGCTG 
CACCGAGCTG GGGGTGACCT TATGCACCGT GATGAGCGGA GCCGCACGCT CCTGCACCAC 
GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC CGCTACCTGC TGGAGCACGC GCCCACTGAG 
ATCCTTGATG CCGTGGAGGA AAACGGGGAG ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC 
CAGCGCACCA TCTGCCACTA CATCGTGGAG GCCGGGGCCT CGCTCATGAA GACTGACCAG 
CAGGGTGACA CTCCCCGGCA GAGAGCGGAG AAGGCTCAGG ACACGGAACT GGCGGCGTAC 
CTGGAAAACC GGCAGCATTA CCAGATGATC CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830960.1
CDS	216..3035
Translation	MSSEPLSCQSLWTRSFKPCFAEEKIETPKGQGAVHGLPQRPDEGFQTSSSTVVHGLSILASSERKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975192.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975192.1

ORF Insert Sequence:

ATGTCATCTG AACCTCTCTC ATGTCAAAGC CTCTGGACTA GGTCGTTTAA GCCCTGTTTT 
GCAGAGGAGA AAATTGAGAC TCCAAAAGGT CAAGGTGCAG TTCACGGGCT CCCTCAGCGT 
CCAGACGAAG GTTTTCAGAC ATCGTCCAGC ACTGTCGTAC ATGGGTTGTC CATCCTGGCC 
TCTTCAGAAA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGCAGA AATCAGTGTC CCGGAGAAAG 
TGTGCAGCCT GCAAGATTGT GGTCCACACA CCCTGCATTG AGCAGCTCGA GAAGATAAAT 
TTCCGCTGTA AGCCATCCTT CCGTGAATCG GGCTCCAGGA ACGTCCGTGA GCCAACCTTC 
GTGCGGCACC ACTGGGTACA CCGGCGACGC CAGGACGGCA AGTGTCGGCA CTGCGGGAAG 
GGCTTCCAGC AGAAGTTCAC CTTCCACAGC AAGGAGATCG TGGCCATCAG CTGCTCCTGG 
TGCAAGCAAG CATACCACAG CAAGGTGTCC TGCTTCATGC TGCAGCAGAT CGAGGAGCCG 
TGCTCCCTGG GGGTCCACGC CGCTGTGGTC ATCCCCCCCA CCTGGATCCT CCGGGCCCGC 
AGGCCCCAGA ACACCCTCAA AGCCAGCAAG AAGAAAAAAA GAGCATCCTT CAAGAGGAAG 
TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC TGGAGACCCT TCATCATCAG GCCTACCCCG 
TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC GTGAACCCCA AGAGTGGGGG CAACCAGGGC 
GCCAAGATCA TCCAGTCCTT CCTCTGGTAT CTGAATCCCC GGCAAGTCTT TGACCTGAGC 
CAGGGGGGCC CCAGGGAGGC GCTGGAGATG TATCGCCGAG TGCACAACCT GCGGATCCTG 
GCCTGCGGGG GTGACGGCAC GGTCGGCTGG ATCCTCTCCA CGCTGGACCA GCTGCGCTTG 
AAGCCGCCGC CGCCAGTCGC CATCCTGCCC CTGGGCACTG GCAATGACTT GGCCCGCACC 
CTCAACTGGG GCGGGGGCTA CACTGACGAG CCTGTGTCCA AGATCCTGTC CCACGTGGAG 
GAGGGCAACG TGGTACAGCT GGACCGCTGG GACCTCCATG CGGAGCCCAA CCCCGAGGCG 
GGGCCCGAGG AGCGAGATGA GGGGGCCACC GACCAGCTGC CTCTGGATGT CTTCAACAAC 
TACTTCAGCC TGGGCTTTGA CGCCCACGTC ACCCTGGAGT TCCACGAGTC TCGAGAGGCC 
AACCCGGAGA AGTTCAACAG CCGCTTCCGG AATAAGATGT TCTACGCCGG GACAGCCTTC 
TCTGACTTCC TGATGGGCAG CTCCAAGGAC TTGGCCAAGC ACATCCGCGT GGTGTGTGAT 
GGGACTGACC TGACCCCCAA GATTCAGGAC CTGAAACCCC AGTGCATTGT TTTCTTGAAC 
ATCCCCAGGT ACTGCGCGGG CACCATGCCC TGGGGCCACC CTGGGGAGCA CCATGACTTT 
GAGCCCCAGC GGCACGACGA TGGCTACCTC GAGGTCATCG GCTTTACCAT GACCTCCCTG 
GCTGCGCTGC AGGTGGGCGG GCACGGCGAG CGGCTGACGC AGTGCCGAGA GGTGCTGCTC 
ACCACGTCCA AAGCCATCCC GGTGCAGGTG GACGGTGAGC CCTGCAAGCT CGCAGCCTCG 
CGCATCCGCA TTGCCCTGCG CAACCAGGCC ACCATGGTGC AGAAGGCCAA GCGGCGGAGC 
GCCGCCCCCC TGCACAGCGA CCAGCAGCCG GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC 
AGGGTCAGCA TGCACGACTA CGAGGCCCTG CACTACGACA AGGAGCAGCT CAAAGAGGCT 
TCTGTGCCGC TGGGCACTGT GGTGGTCCCA GGAGACAGCG ACCTGGAGCT GTGCCGCGCT 
CACATCGAGA GGCTCCGGCA GGAGCCCGAA GGTGCTGGAG CCAAGTCCCC GATGTGCCAG 
AAACTGTCCC CCAAGTGGTG CTTCCTCGAT GCCACCACTG CCAGCCGCTT CTACAGAATC 
GACAGGGCCC AGGAACACCT CAACTACGTG ACCGAGATCG CACAGGACGA GATTTATATC 
CTGGACCCTG AGCTGCTGGG GGCATCTGCC CGTCCTGACC TCCCCACCCC CACGTCCCCT 
CTCCCCACCT CGCCCTGCTC CCCCACATCC CGGTCACTGC CAGGGGACGC TGCGCCCCCT 
ACAGGTGAAG AGCTCATCGA GGCTGCAAAG AGGAACGATT TCTGTAAGCT CCAGGAGCTG 
CACCGAGCTG GGGGTGACCT TATGCACCGT GATGAGCGGA GCCGCACGCT CCTGCACCAC 
GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC CGCTACCTGC TGGAGCACGC GCCCACTGAG 
ATCCTTGATG CCGTGGAGGA AAACGGGGAG ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC 
CAGCGCACCA TCTGCCACTA CATCGTGGAG GCCGGGGCCT CGCTCATGAA GACTGACCAG 
CAGGGTGACA CTCCCCGGCA GAGAGCGGAG AAGGCTCAGG ACACGGAACT GGCGGCGTAC 
CTGGAAAACC GGCAGCATTA CCAGATGATC CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975193.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830961.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa222725
Clone ID Related Accession (Same CDS sequence)	XM_024975193.1
Accession Version	XM_024975193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAT GGACTATAGT CCACCAGGCT CCTCCATCCA TGGGATTCTT CAGGCAAGAA 
TACTGGAGTG GGTTGCCATG CCCTGCTCCA GGAGATCTTC CCAACCCAGG GATCGAACCC 
ACGTTTCTTA CGTCTCCTGC TTTGGCTGGC GGGTTCTTTA CCACTGAGCC CCTGGGAGCA 
GAGACAGACT CTGGTCAAGG TGCAGTTCAC GGGCTCCCTC AGCGTCCAGA CGAAGGTTTT 
CAGACATCGT CCAGCACTGT CGTACATGGG TTGTCCATCC TGGCCTCTTC AGAAAGGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GCAGAAATCA GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG 
ATTGTGGTCC ACACACCCTG CATTGAGCAG CTCGAGAAGA TAAATTTCCG CTGTAAGCCA 
TCCTTCCGTG AATCGGGCTC CAGGAACGTC CGTGAGCCAA CCTTCGTGCG GCACCACTGG 
GTACACCGGC GACGCCAGGA CGGCAAGTGT CGGCACTGCG GGAAGGGCTT CCAGCAGAAG 
TTCACCTTCC ACAGCAAGGA GATCGTGGCC ATCAGCTGCT CCTGGTGCAA GCAAGCATAC 
CACAGCAAGG TGTCCTGCTT CATGCTGCAG CAGATCGAGG AGCCGTGCTC CCTGGGGGTC 
CACGCCGCTG TGGTCATCCC CCCCACCTGG ATCCTCCGGG CCCGCAGGCC CCAGAACACC 
CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG 
CCTGAGGAGG GCCGCTGGAG ACCCTTCATC ATCAGGCCTA CCCCGTCCCC CCTCATGAAG 
CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT GGGGGCAACC AGGGCGCCAA GATCATCCAG 
TCCTTCCTCT GGTATCTGAA TCCCCGGCAA GTCTTTGACC TGAGCCAGGG GGGCCCCAGG 
GAGGCGCTGG AGATGTATCG CCGAGTGCAC AACCTGCGGA TCCTGGCCTG CGGGGGTGAC 
GGCACGGTCG GCTGGATCCT CTCCACGCTG GACCAGCTGC GCTTGAAGCC GCCGCCGCCA 
GTCGCCATCC TGCCCCTGGG CACTGGCAAT GACTTGGCCC GCACCCTCAA CTGGGGCGGG 
GGCTACACTG ACGAGCCTGT GTCCAAGATC CTGTCCCACG TGGAGGAGGG CAACGTGGTA 
CAGCTGGACC GCTGGGACCT CCATGCGGAG CCCAACCCCG AGGCGGGGCC CGAGGAGCGA 
GATGAGGGGG CCACCGACCA GCTGCCTCTG GATGTCTTCA ACAACTACTT CAGCCTGGGC 
TTTGACGCCC ACGTCACCCT GGAGTTCCAC GAGTCTCGAG AGGCCAACCC GGAGAAGTTC 
AACAGCCGCT TCCGGAATAA GATGTTCTAC GCCGGGACAG CCTTCTCTGA CTTCCTGATG 
GGCAGCTCCA AGGACTTGGC CAAGCACATC CGCGTGGTGT GTGATGGGAC TGACCTGACC 
CCCAAGATTC AGGACCTGAA ACCCCAGTGC ATTGTTTTCT TGAACATCCC CAGGTACTGC 
GCGGGCACCA TGCCCTGGGG CCACCCTGGG GAGCACCATG ACTTTGAGCC CCAGCGGCAC 
GACGATGGCT ACCTCGAGGT CATCGGCTTT ACCATGACCT CCCTGGCTGC GCTGCAGGTG 
GGCGGGCACG GCGAGCGGCT GACGCAGTGC CGAGAGGTGC TGCTCACCAC GTCCAAAGCC 
ATCCCGGTGC AGGTGGACGG TGAGCCCTGC AAGCTCGCAG CCTCGCGCAT CCGCATTGCC 
CTGCGCAACC AGGCCACCAT GGTGCAGAAG GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC 
AGCGACCAGC AGCCGGTGCC GGAGCAGCTG CGAGTCCAGG TGAGCAGGGT CAGCATGCAC 
GACTACGAGG CCCTGCACTA CGACAAGGAG CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC 
ACTGTGGTGG TCCCAGGAGA CAGCGACCTG GAGCTGTGCC GCGCTCACAT CGAGAGGCTC 
CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG TCCCCGATGT GCCAGAAACT GTCCCCCAAG 
TGGTGCTTCC TCGATGCCAC CACTGCCAGC CGCTTCTACA GAATCGACAG GGCCCAGGAA 
CACCTCAACT ACGTGACCGA GATCGCACAG GACGAGATTT ATATCCTGGA CCCTGAGCTG 
CTGGGGGCAT CTGCCCGTCC TGACCTCCCC ACCCCCACGT CCCCTCTCCC CACCTCGCCC 
TGCTCCCCCA CATCCCGGTC ACTGCCAGGG GACGCTGCGC CCCCTACAGG TGAAGAGCTC 
ATCGAGGCTG CAAAGAGGAA CGATTTCTGT AAGCTCCAGG AGCTGCACCG AGCTGGGGGT 
GACCTTATGC ACCGTGATGA GCGGAGCCGC ACGCTCCTGC ACCACGCGGT CAGCACCGGC 
AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG CACGCGCCCA CTGAGATCCT TGATGCCGTG 
GAGGAAAACG GGGAGACCTG CCTGCACCAG GCGGCAGCCC TGGGCCAGCG CACCATCTGC 
CACTACATCG TGGAGGCCGG GGCCTCGCTC ATGAAGACTG ACCAGCAGGG TGACACTCCC 
CGGCAGAGAG CGGAGAAGGC TCAGGACACG GAACTGGCGG CGTACCTGGA AAACCGGCAG 
CATTACCAGA TGATCCAGCG GGAGGACCAG GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830961.1
CDS	745..3669
Translation	MTPWTIVHQAPPSMGFFRQEYWSGLPCPAPGDLPNPGIEPTFLTSPALAGGFFTTEPLGAETDSGQGAVHGLPQRPDEGFQTSSSTVVHGLSILASSERKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975193.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975193.1

ORF Insert Sequence:

ATGACCCCAT GGACTATAGT CCACCAGGCT CCTCCATCCA TGGGATTCTT CAGGCAAGAA 
TACTGGAGTG GGTTGCCATG CCCTGCTCCA GGAGATCTTC CCAACCCAGG GATCGAACCC 
ACGTTTCTTA CGTCTCCTGC TTTGGCTGGC GGGTTCTTTA CCACTGAGCC CCTGGGAGCA 
GAGACAGACT CTGGTCAAGG TGCAGTTCAC GGGCTCCCTC AGCGTCCAGA CGAAGGTTTT 
CAGACATCGT CCAGCACTGT CGTACATGGG TTGTCCATCC TGGCCTCTTC AGAAAGGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GCAGAAATCA GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG 
ATTGTGGTCC ACACACCCTG CATTGAGCAG CTCGAGAAGA TAAATTTCCG CTGTAAGCCA 
TCCTTCCGTG AATCGGGCTC CAGGAACGTC CGTGAGCCAA CCTTCGTGCG GCACCACTGG 
GTACACCGGC GACGCCAGGA CGGCAAGTGT CGGCACTGCG GGAAGGGCTT CCAGCAGAAG 
TTCACCTTCC ACAGCAAGGA GATCGTGGCC ATCAGCTGCT CCTGGTGCAA GCAAGCATAC 
CACAGCAAGG TGTCCTGCTT CATGCTGCAG CAGATCGAGG AGCCGTGCTC CCTGGGGGTC 
CACGCCGCTG TGGTCATCCC CCCCACCTGG ATCCTCCGGG CCCGCAGGCC CCAGAACACC 
CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG 
CCTGAGGAGG GCCGCTGGAG ACCCTTCATC ATCAGGCCTA CCCCGTCCCC CCTCATGAAG 
CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT GGGGGCAACC AGGGCGCCAA GATCATCCAG 
TCCTTCCTCT GGTATCTGAA TCCCCGGCAA GTCTTTGACC TGAGCCAGGG GGGCCCCAGG 
GAGGCGCTGG AGATGTATCG CCGAGTGCAC AACCTGCGGA TCCTGGCCTG CGGGGGTGAC 
GGCACGGTCG GCTGGATCCT CTCCACGCTG GACCAGCTGC GCTTGAAGCC GCCGCCGCCA 
GTCGCCATCC TGCCCCTGGG CACTGGCAAT GACTTGGCCC GCACCCTCAA CTGGGGCGGG 
GGCTACACTG ACGAGCCTGT GTCCAAGATC CTGTCCCACG TGGAGGAGGG CAACGTGGTA 
CAGCTGGACC GCTGGGACCT CCATGCGGAG CCCAACCCCG AGGCGGGGCC CGAGGAGCGA 
GATGAGGGGG CCACCGACCA GCTGCCTCTG GATGTCTTCA ACAACTACTT CAGCCTGGGC 
TTTGACGCCC ACGTCACCCT GGAGTTCCAC GAGTCTCGAG AGGCCAACCC GGAGAAGTTC 
AACAGCCGCT TCCGGAATAA GATGTTCTAC GCCGGGACAG CCTTCTCTGA CTTCCTGATG 
GGCAGCTCCA AGGACTTGGC CAAGCACATC CGCGTGGTGT GTGATGGGAC TGACCTGACC 
CCCAAGATTC AGGACCTGAA ACCCCAGTGC ATTGTTTTCT TGAACATCCC CAGGTACTGC 
GCGGGCACCA TGCCCTGGGG CCACCCTGGG GAGCACCATG ACTTTGAGCC CCAGCGGCAC 
GACGATGGCT ACCTCGAGGT CATCGGCTTT ACCATGACCT CCCTGGCTGC GCTGCAGGTG 
GGCGGGCACG GCGAGCGGCT GACGCAGTGC CGAGAGGTGC TGCTCACCAC GTCCAAAGCC 
ATCCCGGTGC AGGTGGACGG TGAGCCCTGC AAGCTCGCAG CCTCGCGCAT CCGCATTGCC 
CTGCGCAACC AGGCCACCAT GGTGCAGAAG GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC 
AGCGACCAGC AGCCGGTGCC GGAGCAGCTG CGAGTCCAGG TGAGCAGGGT CAGCATGCAC 
GACTACGAGG CCCTGCACTA CGACAAGGAG CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC 
ACTGTGGTGG TCCCAGGAGA CAGCGACCTG GAGCTGTGCC GCGCTCACAT CGAGAGGCTC 
CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG TCCCCGATGT GCCAGAAACT GTCCCCCAAG 
TGGTGCTTCC TCGATGCCAC CACTGCCAGC CGCTTCTACA GAATCGACAG GGCCCAGGAA 
CACCTCAACT ACGTGACCGA GATCGCACAG GACGAGATTT ATATCCTGGA CCCTGAGCTG 
CTGGGGGCAT CTGCCCGTCC TGACCTCCCC ACCCCCACGT CCCCTCTCCC CACCTCGCCC 
TGCTCCCCCA CATCCCGGTC ACTGCCAGGG GACGCTGCGC CCCCTACAGG TGAAGAGCTC 
ATCGAGGCTG CAAAGAGGAA CGATTTCTGT AAGCTCCAGG AGCTGCACCG AGCTGGGGGT 
GACCTTATGC ACCGTGATGA GCGGAGCCGC ACGCTCCTGC ACCACGCGGT CAGCACCGGC 
AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG CACGCGCCCA CTGAGATCCT TGATGCCGTG 
GAGGAAAACG GGGAGACCTG CCTGCACCAG GCGGCAGCCC TGGGCCAGCG CACCATCTGC 
CACTACATCG TGGAGGCCGG GGCCTCGCTC ATGAAGACTG ACCAGCAGGG TGACACTCCC 
CGGCAGAGAG CGGAGAAGGC TCAGGACACG GAACTGGCGG CGTACCTGGA AAACCGGCAG 
CATTACCAGA TGATCCAGCG GGAGGACCAG GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975194.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830962.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa222726
Clone ID Related Accession (Same CDS sequence)	XM_024975194.1
Accession Version	XM_024975194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG GGCCGGGCGG CGGAGGGCCC CGCGGGGACG GGGCTGGTGG CGGCCGGGCG 
GCCGAGGAGG AGGTGGTGCG AAGGCGCTGC CGGCGAGGCC AGGAGGCCGA GGCACCCCGG 
GACCGGGCCG CCGGACCCCC TGTGGAGGAG CGGTTCGGCC AGATGCACCT ACGGAAACAG 
GTGTCTTACA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGCAGA AATCAGTGTC CCGGAGAAAG 
TGTGCAGCCT GCAAGATTGT GGTCCACACA CCCTGCATTG AGCAGCTCGA GAAGATAAAT 
TTCCGCTGTA AGCCATCCTT CCGTGAATCG GGCTCCAGGA ACGTCCGTGA GCCAACCTTC 
GTGCGGCACC ACTGGGTACA CCGGCGACGC CAGGACGGCA AGTGTCGGCA CTGCGGGAAG 
GGCTTCCAGC AGAAGTTCAC CTTCCACAGC AAGGAGATCG TGGCCATCAG CTGCTCCTGG 
TGCAAGCAAG CATACCACAG CAAGGTGTCC TGCTTCATGC TGCAGCAGAT CGAGGAGCCG 
TGCTCCCTGG GGGTCCACGC CGCTGTGGTC ATCCCCCCCA CCTGGATCCT CCGGGCCCGC 
AGGCCCCAGA ACACCCTCAA AGCCAGCAAG AAGAAAAAAA GAGCATCCTT CAAGAGGAAG 
TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC TGGAGACCCT TCATCATCAG GCCTACCCCG 
TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC GTGAACCCCA AGAGTGGGGG CAACCAGGGC 
GCCAAGATCA TCCAGTCCTT CCTCTGGTAT CTGAATCCCC GGCAAGTCTT TGACCTGAGC 
CAGGGGGGCC CCAGGGAGGC GCTGGAGATG TATCGCCGAG TGCACAACCT GCGGATCCTG 
GCCTGCGGGG GTGACGGCAC GGTCGGCTGG ATCCTCTCCA CGCTGGACCA GCTGCGCTTG 
AAGCCGCCGC CGCCAGTCGC CATCCTGCCC CTGGGCACTG GCAATGACTT GGCCCGCACC 
CTCAACTGGG GCGGGGGCTA CACTGACGAG CCTGTGTCCA AGATCCTGTC CCACGTGGAG 
GAGGGCAACG TGGTACAGCT GGACCGCTGG GACCTCCATG CGGAGCCCAA CCCCGAGGCG 
GGGCCCGAGG AGCGAGATGA GGGGGCCACC GACCAGCTGC CTCTGGATGT CTTCAACAAC 
TACTTCAGCC TGGGCTTTGA CGCCCACGTC ACCCTGGAGT TCCACGAGTC TCGAGAGGCC 
AACCCGGAGA AGTTCAACAG CCGCTTCCGG AATAAGATGT TCTACGCCGG GACAGCCTTC 
TCTGACTTCC TGATGGGCAG CTCCAAGGAC TTGGCCAAGC ACATCCGCGT GGTGTGTGAT 
GGGACTGACC TGACCCCCAA GATTCAGGAC CTGAAACCCC AGTGCATTGT TTTCTTGAAC 
ATCCCCAGGT ACTGCGCGGG CACCATGCCC TGGGGCCACC CTGGGGAGCA CCATGACTTT 
GAGCCCCAGC GGCACGACGA TGGCTACCTC GAGGTCATCG GCTTTACCAT GACCTCCCTG 
GCTGCGCTGC AGGTGGGCGG GCACGGCGAG CGGCTGACGC AGTGCCGAGA GGTGCTGCTC 
ACCACGTCCA AAGCCATCCC GGTGCAGGTG GACGGTGAGC CCTGCAAGCT CGCAGCCTCG 
CGCATCCGCA TTGCCCTGCG CAACCAGGCC ACCATGGTGC AGAAGGCCAA GCGGCGGAGC 
GCCGCCCCCC TGCACAGCGA CCAGCAGCCG GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC 
AGGGTCAGCA TGCACGACTA CGAGGCCCTG CACTACGACA AGGAGCAGCT CAAAGAGGCT 
TCTGTGCCGC TGGGCACTGT GGTGGTCCCA GGAGACAGCG ACCTGGAGCT GTGCCGCGCT 
CACATCGAGA GGCTCCGGCA GGAGCCCGAA GGTGCTGGAG CCAAGTCCCC GATGTGCCAG 
AAACTGTCCC CCAAGTGGTG CTTCCTCGAT GCCACCACTG CCAGCCGCTT CTACAGAATC 
GACAGGGCCC AGGAACACCT CAACTACGTG ACCGAGATCG CACAGGACGA GATTTATATC 
CTGGACCCTG AGCTGCTGGG GGCATCTGCC CGTCCTGACC TCCCCACCCC CACGTCCCCT 
CTCCCCACCT CGCCCTGCTC CCCCACATCC CGGTCACTGC CAGGGGACGC TGCGCCCCCT 
ACAGGTGAAG AGCTCATCGA GGCTGCAAAG AGGAACGATT TCTGTAAGCT CCAGGAGCTG 
CACCGAGCTG GGGGTGACCT TATGCACCGT GATGAGCGGA GCCGCACGCT CCTGCACCAC 
GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC CGCTACCTGC TGGAGCACGC GCCCACTGAG 
ATCCTTGATG CCGTGGAGGA AAACGGGGAG ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC 
CAGCGCACCA TCTGCCACTA CATCGTGGAG GCCGGGGCCT CGCTCATGAA GACTGACCAG 
CAGGGTGACA CTCCCCGGCA GAGAGCGGAG AAGGCTCAGG ACACGGAACT GGCGGCGTAC 
CTGGAAAACC GGCAGCATTA CCAGATGATC CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830962.1
CDS	16..2835
Translation	MAEGPGGGGPRGDGAGGGRAAEEEVVRRRCRRGQEAEAPRDRAAGPPVEERFGQMHLRKQVSYRKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975194.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975194.1

ORF Insert Sequence:

ATGGCCGAGG GGCCGGGCGG CGGAGGGCCC CGCGGGGACG GGGCTGGTGG CGGCCGGGCG 
GCCGAGGAGG AGGTGGTGCG AAGGCGCTGC CGGCGAGGCC AGGAGGCCGA GGCACCCCGG 
GACCGGGCCG CCGGACCCCC TGTGGAGGAG CGGTTCGGCC AGATGCACCT ACGGAAACAG 
GTGTCTTACA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGCAGA AATCAGTGTC CCGGAGAAAG 
TGTGCAGCCT GCAAGATTGT GGTCCACACA CCCTGCATTG AGCAGCTCGA GAAGATAAAT 
TTCCGCTGTA AGCCATCCTT CCGTGAATCG GGCTCCAGGA ACGTCCGTGA GCCAACCTTC 
GTGCGGCACC ACTGGGTACA CCGGCGACGC CAGGACGGCA AGTGTCGGCA CTGCGGGAAG 
GGCTTCCAGC AGAAGTTCAC CTTCCACAGC AAGGAGATCG TGGCCATCAG CTGCTCCTGG 
TGCAAGCAAG CATACCACAG CAAGGTGTCC TGCTTCATGC TGCAGCAGAT CGAGGAGCCG 
TGCTCCCTGG GGGTCCACGC CGCTGTGGTC ATCCCCCCCA CCTGGATCCT CCGGGCCCGC 
AGGCCCCAGA ACACCCTCAA AGCCAGCAAG AAGAAAAAAA GAGCATCCTT CAAGAGGAAG 
TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC TGGAGACCCT TCATCATCAG GCCTACCCCG 
TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC GTGAACCCCA AGAGTGGGGG CAACCAGGGC 
GCCAAGATCA TCCAGTCCTT CCTCTGGTAT CTGAATCCCC GGCAAGTCTT TGACCTGAGC 
CAGGGGGGCC CCAGGGAGGC GCTGGAGATG TATCGCCGAG TGCACAACCT GCGGATCCTG 
GCCTGCGGGG GTGACGGCAC GGTCGGCTGG ATCCTCTCCA CGCTGGACCA GCTGCGCTTG 
AAGCCGCCGC CGCCAGTCGC CATCCTGCCC CTGGGCACTG GCAATGACTT GGCCCGCACC 
CTCAACTGGG GCGGGGGCTA CACTGACGAG CCTGTGTCCA AGATCCTGTC CCACGTGGAG 
GAGGGCAACG TGGTACAGCT GGACCGCTGG GACCTCCATG CGGAGCCCAA CCCCGAGGCG 
GGGCCCGAGG AGCGAGATGA GGGGGCCACC GACCAGCTGC CTCTGGATGT CTTCAACAAC 
TACTTCAGCC TGGGCTTTGA CGCCCACGTC ACCCTGGAGT TCCACGAGTC TCGAGAGGCC 
AACCCGGAGA AGTTCAACAG CCGCTTCCGG AATAAGATGT TCTACGCCGG GACAGCCTTC 
TCTGACTTCC TGATGGGCAG CTCCAAGGAC TTGGCCAAGC ACATCCGCGT GGTGTGTGAT 
GGGACTGACC TGACCCCCAA GATTCAGGAC CTGAAACCCC AGTGCATTGT TTTCTTGAAC 
ATCCCCAGGT ACTGCGCGGG CACCATGCCC TGGGGCCACC CTGGGGAGCA CCATGACTTT 
GAGCCCCAGC GGCACGACGA TGGCTACCTC GAGGTCATCG GCTTTACCAT GACCTCCCTG 
GCTGCGCTGC AGGTGGGCGG GCACGGCGAG CGGCTGACGC AGTGCCGAGA GGTGCTGCTC 
ACCACGTCCA AAGCCATCCC GGTGCAGGTG GACGGTGAGC CCTGCAAGCT CGCAGCCTCG 
CGCATCCGCA TTGCCCTGCG CAACCAGGCC ACCATGGTGC AGAAGGCCAA GCGGCGGAGC 
GCCGCCCCCC TGCACAGCGA CCAGCAGCCG GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC 
AGGGTCAGCA TGCACGACTA CGAGGCCCTG CACTACGACA AGGAGCAGCT CAAAGAGGCT 
TCTGTGCCGC TGGGCACTGT GGTGGTCCCA GGAGACAGCG ACCTGGAGCT GTGCCGCGCT 
CACATCGAGA GGCTCCGGCA GGAGCCCGAA GGTGCTGGAG CCAAGTCCCC GATGTGCCAG 
AAACTGTCCC CCAAGTGGTG CTTCCTCGAT GCCACCACTG CCAGCCGCTT CTACAGAATC 
GACAGGGCCC AGGAACACCT CAACTACGTG ACCGAGATCG CACAGGACGA GATTTATATC 
CTGGACCCTG AGCTGCTGGG GGCATCTGCC CGTCCTGACC TCCCCACCCC CACGTCCCCT 
CTCCCCACCT CGCCCTGCTC CCCCACATCC CGGTCACTGC CAGGGGACGC TGCGCCCCCT 
ACAGGTGAAG AGCTCATCGA GGCTGCAAAG AGGAACGATT TCTGTAAGCT CCAGGAGCTG 
CACCGAGCTG GGGGTGACCT TATGCACCGT GATGAGCGGA GCCGCACGCT CCTGCACCAC 
GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC CGCTACCTGC TGGAGCACGC GCCCACTGAG 
ATCCTTGATG CCGTGGAGGA AAACGGGGAG ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC 
CAGCGCACCA TCTGCCACTA CATCGTGGAG GCCGGGGCCT CGCTCATGAA GACTGACCAG 
CAGGGTGACA CTCCCCGGCA GAGAGCGGAG AAGGCTCAGG ACACGGAACT GGCGGCGTAC 
CTGGAAAACC GGCAGCATTA CCAGATGATC CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975195.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830963.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa222727
Clone ID Related Accession (Same CDS sequence)	XM_024975195.1
Accession Version	XM_024975195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG GGCCGGGCGG CGGAGGGCCC CGCGGGGACG GGGCTGGTGG CGGCCGGGCG 
GCCGAGGAGG AGGTGGTGCG AAGGCGCTGC CGGCGAGGCC AGGAGGCCGA GGCACCCCGG 
GACCGGGCCG CCGGACCCCC TGTGGAGGAG CGGTTCGGCC AGATGCACCT ACGGAAACAG 
GTGTCTTACA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT 
GCAGCCTGCA AGATTGTGGT CCACACACCC TGCATTGAGC AGCTCGAGAA GATAAATTTC 
CGCTGTAAGC CATCCTTCCG TGAATCGGGC TCCAGGAACG TCCGTGAGCC AACCTTCGTG 
CGGCACCACT GGGTACACCG GCGACGCCAG GACGGCAAGT GTCGGCACTG CGGGAAGGGC 
TTCCAGCAGA AGTTCACCTT CCACAGCAAG GAGATCGTGG CCATCAGCTG CTCCTGGTGC 
AAGCAAGCAT ACCACAGCAA GGTGTCCTGC TTCATGCTGC AGCAGATCGA GGAGCCGTGC 
TCCCTGGGGG TCCACGCCGC TGTGGTCATC CCCCCCACCT GGATCCTCCG GGCCCGCAGG 
CCCCAGAACA CCCTCAAAGC CAGCAAGAAG AAAAAAAGAG CATCCTTCAA GAGGAAGTCT 
AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG AGACCCTTCA TCATCAGGCC TACCCCGTCC 
CCCCTCATGA AGCCCCTGCT GGTGTTCGTG AACCCCAAGA GTGGGGGCAA CCAGGGCGCC 
AAGATCATCC AGTCCTTCCT CTGGTATCTG AATCCCCGGC AAGTCTTTGA CCTGAGCCAG 
GGGGGCCCCA GGGAGGCGCT GGAGATGTAT CGCCGAGTGC ACAACCTGCG GATCCTGGCC 
TGCGGGGGTG ACGGCACGGT CGGCTGGATC CTCTCCACGC TGGACCAGCT GCGCTTGAAG 
CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG GGCACTGGCA ATGACTTGGC CCGCACCCTC 
AACTGGGGCG GGGGCTACAC TGACGAGCCT GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG 
GGCAACGTGG TACAGCTGGA CCGCTGGGAC CTCCATGCGG AGCCCAACCC CGAGGCGGGG 
CCCGAGGAGC GAGATGAGGG GGCCACCGAC CAGCTGCCTC TGGATGTCTT CAACAACTAC 
TTCAGCCTGG GCTTTGACGC CCACGTCACC CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC 
CCGGAGAAGT TCAACAGCCG CTTCCGGAAT AAGATGTTCT ACGCCGGGAC AGCCTTCTCT 
GACTTCCTGA TGGGCAGCTC CAAGGACTTG GCCAAGCACA TCCGCGTGGT GTGTGATGGG 
ACTGACCTGA CCCCCAAGAT TCAGGACCTG AAACCCCAGT GCATTGTTTT CTTGAACATC 
CCCAGGTACT GCGCGGGCAC CATGCCCTGG GGCCACCCTG GGGAGCACCA TGACTTTGAG 
CCCCAGCGGC ACGACGATGG CTACCTCGAG GTCATCGGCT TTACCATGAC CTCCCTGGCT 
GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG CTGACGCAGT GCCGAGAGGT GCTGCTCACC 
ACGTCCAAAG CCATCCCGGT GCAGGTGGAC GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC 
ATCCGCATTG CCCTGCGCAA CCAGGCCACC ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC 
GCCCCCCTGC ACAGCGACCA GCAGCCGGTG CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG 
GTCAGCATGC ACGACTACGA GGCCCTGCAC TACGACAAGG AGCAGCTCAA AGAGGCTTCT 
GTGCCGCTGG GCACTGTGGT GGTCCCAGGA GACAGCGACC TGGAGCTGTG CCGCGCTCAC 
ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA 
CTGTCCCCCA AGTGGTGCTT CCTCGATGCC ACCACTGCCA GCCGCTTCTA CAGAATCGAC 
AGGGCCCAGG AACACCTCAA CTACGTGACC GAGATCGCAC AGGACGAGAT TTATATCCTG 
GACCCTGAGC TGCTGGGGGC ATCTGCCCGT CCTGACCTCC CCACCCCCAC GTCCCCTCTC 
CCCACCTCGC CCTGCTCCCC CACATCCCGG TCACTGCCAG GGGACGCTGC GCCCCCTACA 
GGTGAAGAGC TCATCGAGGC TGCAAAGAGG AACGATTTCT GTAAGCTCCA GGAGCTGCAC 
CGAGCTGGGG GTGACCTTAT GCACCGTGAT GAGCGGAGCC GCACGCTCCT GCACCACGCG 
GTCAGCACCG GCAGCAAGGA AGTGGTCCGC TACCTGCTGG AGCACGCGCC CACTGAGATC 
CTTGATGCCG TGGAGGAAAA CGGGGAGACC TGCCTGCACC AGGCGGCAGC CCTGGGCCAG 
CGCACCATCT GCCACTACAT CGTGGAGGCC GGGGCCTCGC TCATGAAGAC TGACCAGCAG 
GGTGACACTC CCCGGCAGAG AGCGGAGAAG GCTCAGGACA CGGAACTGGC GGCGTACCTG 
GAAAACCGGC AGCATTACCA GATGATCCAG CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830963.1
CDS	16..2832
Translation	MAEGPGGGGPRGDGAGGGRAAEEEVVRRRCRRGQEAEAPRDRAAGPPVEERFGQMHLRKQVSYRKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975195.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975195.1

ORF Insert Sequence:

ATGGCCGAGG GGCCGGGCGG CGGAGGGCCC CGCGGGGACG GGGCTGGTGG CGGCCGGGCG 
GCCGAGGAGG AGGTGGTGCG AAGGCGCTGC CGGCGAGGCC AGGAGGCCGA GGCACCCCGG 
GACCGGGCCG CCGGACCCCC TGTGGAGGAG CGGTTCGGCC AGATGCACCT ACGGAAACAG 
GTGTCTTACA GGAAAGCCAT CACCAAGTCG GGCCTCCAGC ACCTGGCACC CCCTCCTCCT 
GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG CGGCAGATCC GGAGCACCGT GGACTGGAGT 
GAGTCAGCGA CGTATGGGGA ACACATCTGG TTTGAGACCA ACGTGTCCGG GGACTTCTGC 
TACGTCGGAG AGCAGTACTG CGTCGCCAAG ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT 
GCAGCCTGCA AGATTGTGGT CCACACACCC TGCATTGAGC AGCTCGAGAA GATAAATTTC 
CGCTGTAAGC CATCCTTCCG TGAATCGGGC TCCAGGAACG TCCGTGAGCC AACCTTCGTG 
CGGCACCACT GGGTACACCG GCGACGCCAG GACGGCAAGT GTCGGCACTG CGGGAAGGGC 
TTCCAGCAGA AGTTCACCTT CCACAGCAAG GAGATCGTGG CCATCAGCTG CTCCTGGTGC 
AAGCAAGCAT ACCACAGCAA GGTGTCCTGC TTCATGCTGC AGCAGATCGA GGAGCCGTGC 
TCCCTGGGGG TCCACGCCGC TGTGGTCATC CCCCCCACCT GGATCCTCCG GGCCCGCAGG 
CCCCAGAACA CCCTCAAAGC CAGCAAGAAG AAAAAAAGAG CATCCTTCAA GAGGAAGTCT 
AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG AGACCCTTCA TCATCAGGCC TACCCCGTCC 
CCCCTCATGA AGCCCCTGCT GGTGTTCGTG AACCCCAAGA GTGGGGGCAA CCAGGGCGCC 
AAGATCATCC AGTCCTTCCT CTGGTATCTG AATCCCCGGC AAGTCTTTGA CCTGAGCCAG 
GGGGGCCCCA GGGAGGCGCT GGAGATGTAT CGCCGAGTGC ACAACCTGCG GATCCTGGCC 
TGCGGGGGTG ACGGCACGGT CGGCTGGATC CTCTCCACGC TGGACCAGCT GCGCTTGAAG 
CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG GGCACTGGCA ATGACTTGGC CCGCACCCTC 
AACTGGGGCG GGGGCTACAC TGACGAGCCT GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG 
GGCAACGTGG TACAGCTGGA CCGCTGGGAC CTCCATGCGG AGCCCAACCC CGAGGCGGGG 
CCCGAGGAGC GAGATGAGGG GGCCACCGAC CAGCTGCCTC TGGATGTCTT CAACAACTAC 
TTCAGCCTGG GCTTTGACGC CCACGTCACC CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC 
CCGGAGAAGT TCAACAGCCG CTTCCGGAAT AAGATGTTCT ACGCCGGGAC AGCCTTCTCT 
GACTTCCTGA TGGGCAGCTC CAAGGACTTG GCCAAGCACA TCCGCGTGGT GTGTGATGGG 
ACTGACCTGA CCCCCAAGAT TCAGGACCTG AAACCCCAGT GCATTGTTTT CTTGAACATC 
CCCAGGTACT GCGCGGGCAC CATGCCCTGG GGCCACCCTG GGGAGCACCA TGACTTTGAG 
CCCCAGCGGC ACGACGATGG CTACCTCGAG GTCATCGGCT TTACCATGAC CTCCCTGGCT 
GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG CTGACGCAGT GCCGAGAGGT GCTGCTCACC 
ACGTCCAAAG CCATCCCGGT GCAGGTGGAC GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC 
ATCCGCATTG CCCTGCGCAA CCAGGCCACC ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC 
GCCCCCCTGC ACAGCGACCA GCAGCCGGTG CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG 
GTCAGCATGC ACGACTACGA GGCCCTGCAC TACGACAAGG AGCAGCTCAA AGAGGCTTCT 
GTGCCGCTGG GCACTGTGGT GGTCCCAGGA GACAGCGACC TGGAGCTGTG CCGCGCTCAC 
ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA 
CTGTCCCCCA AGTGGTGCTT CCTCGATGCC ACCACTGCCA GCCGCTTCTA CAGAATCGAC 
AGGGCCCAGG AACACCTCAA CTACGTGACC GAGATCGCAC AGGACGAGAT TTATATCCTG 
GACCCTGAGC TGCTGGGGGC ATCTGCCCGT CCTGACCTCC CCACCCCCAC GTCCCCTCTC 
CCCACCTCGC CCTGCTCCCC CACATCCCGG TCACTGCCAG GGGACGCTGC GCCCCCTACA 
GGTGAAGAGC TCATCGAGGC TGCAAAGAGG AACGATTTCT GTAAGCTCCA GGAGCTGCAC 
CGAGCTGGGG GTGACCTTAT GCACCGTGAT GAGCGGAGCC GCACGCTCCT GCACCACGCG 
GTCAGCACCG GCAGCAAGGA AGTGGTCCGC TACCTGCTGG AGCACGCGCC CACTGAGATC 
CTTGATGCCG TGGAGGAAAA CGGGGAGACC TGCCTGCACC AGGCGGCAGC CCTGGGCCAG 
CGCACCATCT GCCACTACAT CGTGGAGGCC GGGGCCTCGC TCATGAAGAC TGACCAGCAG 
GGTGACACTC CCCGGCAGAG AGCGGAGAAG GCTCAGGACA CGGAACTGGC GGCGTACCTG 
GAAAACCGGC AGCATTACCA GATGATCCAG CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975196.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830964.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa222728
Clone ID Related Accession (Same CDS sequence)	XM_024975196.1
Accession Version	XM_024975196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCAGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GCAGAAATCA GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG 
ATTGTGGTCC ACACACCCTG CATTGAGCAG CTCGAGAAGA TAAATTTCCG CTGTAAGCCA 
TCCTTCCGTG AATCGGGCTC CAGGAACGTC CGTGAGCCAA CCTTCGTGCG GCACCACTGG 
GTACACCGGC GACGCCAGGA CGGCAAGTGT CGGCACTGCG GGAAGGGCTT CCAGCAGAAG 
TTCACCTTCC ACAGCAAGGA GATCGTGGCC ATCAGCTGCT CCTGGTGCAA GCAAGCATAC 
CACAGCAAGG TGTCCTGCTT CATGCTGCAG CAGATCGAGG AGCCGTGCTC CCTGGGGGTC 
CACGCCGCTG TGGTCATCCC CCCCACCTGG ATCCTCCGGG CCCGCAGGCC CCAGAACACC 
CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG 
CCTGAGGAGG GCCGCTGGAG ACCCTTCATC ATCAGGCCTA CCCCGTCCCC CCTCATGAAG 
CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT GGGGGCAACC AGGGCGCCAA GATCATCCAG 
TCCTTCCTCT GGTATCTGAA TCCCCGGCAA GTCTTTGACC TGAGCCAGGG GGGCCCCAGG 
GAGGCGCTGG AGATGTATCG CCGAGTGCAC AACCTGCGGA TCCTGGCCTG CGGGGGTGAC 
GGCACGGTCG GCTGGATCCT CTCCACGCTG GACCAGCTGC GCTTGAAGCC GCCGCCGCCA 
GTCGCCATCC TGCCCCTGGG CACTGGCAAT GACTTGGCCC GCACCCTCAA CTGGGGCGGG 
GGCTACACTG ACGAGCCTGT GTCCAAGATC CTGTCCCACG TGGAGGAGGG CAACGTGGTA 
CAGCTGGACC GCTGGGACCT CCATGCGGAG CCCAACCCCG AGGCGGGGCC CGAGGAGCGA 
GATGAGGGGG CCACCGACCA GCTGCCTCTG GATGTCTTCA ACAACTACTT CAGCCTGGGC 
TTTGACGCCC ACGTCACCCT GGAGTTCCAC GAGTCTCGAG AGGCCAACCC GGAGAAGTTC 
AACAGCCGCT TCCGGAATAA GATGTTCTAC GCCGGGACAG CCTTCTCTGA CTTCCTGATG 
GGCAGCTCCA AGGACTTGGC CAAGCACATC CGCGTGGTGT GTGATGGGAC TGACCTGACC 
CCCAAGATTC AGGACCTGAA ACCCCAGTGC ATTGTTTTCT TGAACATCCC CAGGTACTGC 
GCGGGCACCA TGCCCTGGGG CCACCCTGGG GAGCACCATG ACTTTGAGCC CCAGCGGCAC 
GACGATGGCT ACCTCGAGGT CATCGGCTTT ACCATGACCT CCCTGGCTGC GCTGCAGGTG 
GGCGGGCACG GCGAGCGGCT GACGCAGTGC CGAGAGGTGC TGCTCACCAC GTCCAAAGCC 
ATCCCGGTGC AGGTGGACGG TGAGCCCTGC AAGCTCGCAG CCTCGCGCAT CCGCATTGCC 
CTGCGCAACC AGGCCACCAT GGTGCAGAAG GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC 
AGCGACCAGC AGCCGGTGCC GGAGCAGCTG CGAGTCCAGG TGAGCAGGGT CAGCATGCAC 
GACTACGAGG CCCTGCACTA CGACAAGGAG CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC 
ACTGTGGTGG TCCCAGGAGA CAGCGACCTG GAGCTGTGCC GCGCTCACAT CGAGAGGCTC 
CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG TCCCCGATGT GCCAGAAACT GTCCCCCAAG 
TGGTGCTTCC TCGATGCCAC CACTGCCAGC CGCTTCTACA GAATCGACAG GGCCCAGGAA 
CACCTCAACT ACGTGACCGA GATCGCACAG GACGAGATTT ATATCCTGGA CCCTGAGCTG 
CTGGGGGCAT CTGCCCGTCC TGACCTCCCC ACCCCCACGT CCCCTCTCCC CACCTCGCCC 
TGCTCCCCCA CATCCCGGTC ACTGCCAGGG GACGCTGCGC CCCCTACAGG TGAAGAGCTC 
ATCGAGGCTG CAAAGAGGAA CGATTTCTGT AAGCTCCAGG AGCTGCACCG AGCTGGGGGT 
GACCTTATGC ACCGTGATGA GCGGAGCCGC ACGCTCCTGC ACCACGCGGT CAGCACCGGC 
AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG CACGCGCCCA CTGAGATCCT TGATGCCGTG 
GAGGAAAACG GGGAGACCTG CCTGCACCAG GCGGCAGCCC TGGGCCAGCG CACCATCTGC 
CACTACATCG TGGAGGCCGG GGCCTCGCTC ATGAAGACTG ACCAGCAGGG TGACACTCCC 
CGGCAGAGAG CGGAGAAGGC TCAGGACACG GAACTGGCGG CGTACCTGGA AAACCGGCAG 
CATTACCAGA TGATCCAGCG GGAGGACCAG GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830964.1
CDS	16..2820
Translation	MSARGAGRSTGGGCDEAAALGPAELLATEEGERPGALRQMWRYRSWDVPQIPAEVPQSQKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975196.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975196.1

ORF Insert Sequence:

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCAGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GCAGAAATCA GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG 
ATTGTGGTCC ACACACCCTG CATTGAGCAG CTCGAGAAGA TAAATTTCCG CTGTAAGCCA 
TCCTTCCGTG AATCGGGCTC CAGGAACGTC CGTGAGCCAA CCTTCGTGCG GCACCACTGG 
GTACACCGGC GACGCCAGGA CGGCAAGTGT CGGCACTGCG GGAAGGGCTT CCAGCAGAAG 
TTCACCTTCC ACAGCAAGGA GATCGTGGCC ATCAGCTGCT CCTGGTGCAA GCAAGCATAC 
CACAGCAAGG TGTCCTGCTT CATGCTGCAG CAGATCGAGG AGCCGTGCTC CCTGGGGGTC 
CACGCCGCTG TGGTCATCCC CCCCACCTGG ATCCTCCGGG CCCGCAGGCC CCAGAACACC 
CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG 
CCTGAGGAGG GCCGCTGGAG ACCCTTCATC ATCAGGCCTA CCCCGTCCCC CCTCATGAAG 
CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT GGGGGCAACC AGGGCGCCAA GATCATCCAG 
TCCTTCCTCT GGTATCTGAA TCCCCGGCAA GTCTTTGACC TGAGCCAGGG GGGCCCCAGG 
GAGGCGCTGG AGATGTATCG CCGAGTGCAC AACCTGCGGA TCCTGGCCTG CGGGGGTGAC 
GGCACGGTCG GCTGGATCCT CTCCACGCTG GACCAGCTGC GCTTGAAGCC GCCGCCGCCA 
GTCGCCATCC TGCCCCTGGG CACTGGCAAT GACTTGGCCC GCACCCTCAA CTGGGGCGGG 
GGCTACACTG ACGAGCCTGT GTCCAAGATC CTGTCCCACG TGGAGGAGGG CAACGTGGTA 
CAGCTGGACC GCTGGGACCT CCATGCGGAG CCCAACCCCG AGGCGGGGCC CGAGGAGCGA 
GATGAGGGGG CCACCGACCA GCTGCCTCTG GATGTCTTCA ACAACTACTT CAGCCTGGGC 
TTTGACGCCC ACGTCACCCT GGAGTTCCAC GAGTCTCGAG AGGCCAACCC GGAGAAGTTC 
AACAGCCGCT TCCGGAATAA GATGTTCTAC GCCGGGACAG CCTTCTCTGA CTTCCTGATG 
GGCAGCTCCA AGGACTTGGC CAAGCACATC CGCGTGGTGT GTGATGGGAC TGACCTGACC 
CCCAAGATTC AGGACCTGAA ACCCCAGTGC ATTGTTTTCT TGAACATCCC CAGGTACTGC 
GCGGGCACCA TGCCCTGGGG CCACCCTGGG GAGCACCATG ACTTTGAGCC CCAGCGGCAC 
GACGATGGCT ACCTCGAGGT CATCGGCTTT ACCATGACCT CCCTGGCTGC GCTGCAGGTG 
GGCGGGCACG GCGAGCGGCT GACGCAGTGC CGAGAGGTGC TGCTCACCAC GTCCAAAGCC 
ATCCCGGTGC AGGTGGACGG TGAGCCCTGC AAGCTCGCAG CCTCGCGCAT CCGCATTGCC 
CTGCGCAACC AGGCCACCAT GGTGCAGAAG GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC 
AGCGACCAGC AGCCGGTGCC GGAGCAGCTG CGAGTCCAGG TGAGCAGGGT CAGCATGCAC 
GACTACGAGG CCCTGCACTA CGACAAGGAG CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC 
ACTGTGGTGG TCCCAGGAGA CAGCGACCTG GAGCTGTGCC GCGCTCACAT CGAGAGGCTC 
CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG TCCCCGATGT GCCAGAAACT GTCCCCCAAG 
TGGTGCTTCC TCGATGCCAC CACTGCCAGC CGCTTCTACA GAATCGACAG GGCCCAGGAA 
CACCTCAACT ACGTGACCGA GATCGCACAG GACGAGATTT ATATCCTGGA CCCTGAGCTG 
CTGGGGGCAT CTGCCCGTCC TGACCTCCCC ACCCCCACGT CCCCTCTCCC CACCTCGCCC 
TGCTCCCCCA CATCCCGGTC ACTGCCAGGG GACGCTGCGC CCCCTACAGG TGAAGAGCTC 
ATCGAGGCTG CAAAGAGGAA CGATTTCTGT AAGCTCCAGG AGCTGCACCG AGCTGGGGGT 
GACCTTATGC ACCGTGATGA GCGGAGCCGC ACGCTCCTGC ACCACGCGGT CAGCACCGGC 
AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG CACGCGCCCA CTGAGATCCT TGATGCCGTG 
GAGGAAAACG GGGAGACCTG CCTGCACCAG GCGGCAGCCC TGGGCCAGCG CACCATCTGC 
CACTACATCG TGGAGGCCGG GGCCTCGCTC ATGAAGACTG ACCAGCAGGG TGACACTCCC 
CGGCAGAGAG CGGAGAAGGC TCAGGACACG GAACTGGCGG CGTACCTGGA AAACCGGCAG 
CATTACCAGA TGATCCAGCG GGAGGACCAG GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975197.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830965.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa222729
Clone ID Related Accession (Same CDS sequence)	XM_024975197.1
Accession Version	XM_024975197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCAGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GAAATCAGTG TCCCGGAGAA AGTGTGCAGC CTGCAAGATT 
GTGGTCCACA CACCCTGCAT TGAGCAGCTC GAGAAGATAA ATTTCCGCTG TAAGCCATCC 
TTCCGTGAAT CGGGCTCCAG GAACGTCCGT GAGCCAACCT TCGTGCGGCA CCACTGGGTA 
CACCGGCGAC GCCAGGACGG CAAGTGTCGG CACTGCGGGA AGGGCTTCCA GCAGAAGTTC 
ACCTTCCACA GCAAGGAGAT CGTGGCCATC AGCTGCTCCT GGTGCAAGCA AGCATACCAC 
AGCAAGGTGT CCTGCTTCAT GCTGCAGCAG ATCGAGGAGC CGTGCTCCCT GGGGGTCCAC 
GCCGCTGTGG TCATCCCCCC CACCTGGATC CTCCGGGCCC GCAGGCCCCA GAACACCCTC 
AAAGCCAGCA AGAAGAAAAA AAGAGCATCC TTCAAGAGGA AGTCTAGCAA GAAAGGGCCT 
GAGGAGGGCC GCTGGAGACC CTTCATCATC AGGCCTACCC CGTCCCCCCT CATGAAGCCC 
CTGCTGGTGT TCGTGAACCC CAAGAGTGGG GGCAACCAGG GCGCCAAGAT CATCCAGTCC 
TTCCTCTGGT ATCTGAATCC CCGGCAAGTC TTTGACCTGA GCCAGGGGGG CCCCAGGGAG 
GCGCTGGAGA TGTATCGCCG AGTGCACAAC CTGCGGATCC TGGCCTGCGG GGGTGACGGC 
ACGGTCGGCT GGATCCTCTC CACGCTGGAC CAGCTGCGCT TGAAGCCGCC GCCGCCAGTC 
GCCATCCTGC CCCTGGGCAC TGGCAATGAC TTGGCCCGCA CCCTCAACTG GGGCGGGGGC 
TACACTGACG AGCCTGTGTC CAAGATCCTG TCCCACGTGG AGGAGGGCAA CGTGGTACAG 
CTGGACCGCT GGGACCTCCA TGCGGAGCCC AACCCCGAGG CGGGGCCCGA GGAGCGAGAT 
GAGGGGGCCA CCGACCAGCT GCCTCTGGAT GTCTTCAACA ACTACTTCAG CCTGGGCTTT 
GACGCCCACG TCACCCTGGA GTTCCACGAG TCTCGAGAGG CCAACCCGGA GAAGTTCAAC 
AGCCGCTTCC GGAATAAGAT GTTCTACGCC GGGACAGCCT TCTCTGACTT CCTGATGGGC 
AGCTCCAAGG ACTTGGCCAA GCACATCCGC GTGGTGTGTG ATGGGACTGA CCTGACCCCC 
AAGATTCAGG ACCTGAAACC CCAGTGCATT GTTTTCTTGA ACATCCCCAG GTACTGCGCG 
GGCACCATGC CCTGGGGCCA CCCTGGGGAG CACCATGACT TTGAGCCCCA GCGGCACGAC 
GATGGCTACC TCGAGGTCAT CGGCTTTACC ATGACCTCCC TGGCTGCGCT GCAGGTGGGC 
GGGCACGGCG AGCGGCTGAC GCAGTGCCGA GAGGTGCTGC TCACCACGTC CAAAGCCATC 
CCGGTGCAGG TGGACGGTGA GCCCTGCAAG CTCGCAGCCT CGCGCATCCG CATTGCCCTG 
CGCAACCAGG CCACCATGGT GCAGAAGGCC AAGCGGCGGA GCGCCGCCCC CCTGCACAGC 
GACCAGCAGC CGGTGCCGGA GCAGCTGCGA GTCCAGGTGA GCAGGGTCAG CATGCACGAC 
TACGAGGCCC TGCACTACGA CAAGGAGCAG CTCAAAGAGG CTTCTGTGCC GCTGGGCACT 
GTGGTGGTCC CAGGAGACAG CGACCTGGAG CTGTGCCGCG CTCACATCGA GAGGCTCCGG 
CAGGAGCCCG AAGGTGCTGG AGCCAAGTCC CCGATGTGCC AGAAACTGTC CCCCAAGTGG 
TGCTTCCTCG ATGCCACCAC TGCCAGCCGC TTCTACAGAA TCGACAGGGC CCAGGAACAC 
CTCAACTACG TGACCGAGAT CGCACAGGAC GAGATTTATA TCCTGGACCC TGAGCTGCTG 
GGGGCATCTG CCCGTCCTGA CCTCCCCACC CCCACGTCCC CTCTCCCCAC CTCGCCCTGC 
TCCCCCACAT CCCGGTCACT GCCAGGGGAC GCTGCGCCCC CTACAGGTGA AGAGCTCATC 
GAGGCTGCAA AGAGGAACGA TTTCTGTAAG CTCCAGGAGC TGCACCGAGC TGGGGGTGAC 
CTTATGCACC GTGATGAGCG GAGCCGCACG CTCCTGCACC ACGCGGTCAG CACCGGCAGC 
AAGGAAGTGG TCCGCTACCT GCTGGAGCAC GCGCCCACTG AGATCCTTGA TGCCGTGGAG 
GAAAACGGGG AGACCTGCCT GCACCAGGCG GCAGCCCTGG GCCAGCGCAC CATCTGCCAC 
TACATCGTGG AGGCCGGGGC CTCGCTCATG AAGACTGACC AGCAGGGTGA CACTCCCCGG 
CAGAGAGCGG AGAAGGCTCA GGACACGGAA CTGGCGGCGT ACCTGGAAAA CCGGCAGCAT 
TACCAGATGA TCCAGCGGGA GGACCAGGAG ACAGCGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830965.1
CDS	16..2817
Translation	MSARGAGRSTGGGCDEAAALGPAELLATEEGERPGALRQMWRYRSWDVPQIPAEVPQSQKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975197.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975197.1

ORF Insert Sequence:

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCAGAAA 
GCCATCACCA AGTCGGGCCT CCAGCACCTG GCACCCCCTC CTCCTGCTCC TGGGGCCCCG 
TGCAGCGAGC CTGAGCGGCA GATCCGGAGC ACCGTGGACT GGAGTGAGTC AGCGACGTAT 
GGGGAACACA TCTGGTTTGA GACCAACGTG TCCGGGGACT TCTGCTACGT CGGAGAGCAG 
TACTGCGTCG CCAAGATGCT GAAATCAGTG TCCCGGAGAA AGTGTGCAGC CTGCAAGATT 
GTGGTCCACA CACCCTGCAT TGAGCAGCTC GAGAAGATAA ATTTCCGCTG TAAGCCATCC 
TTCCGTGAAT CGGGCTCCAG GAACGTCCGT GAGCCAACCT TCGTGCGGCA CCACTGGGTA 
CACCGGCGAC GCCAGGACGG CAAGTGTCGG CACTGCGGGA AGGGCTTCCA GCAGAAGTTC 
ACCTTCCACA GCAAGGAGAT CGTGGCCATC AGCTGCTCCT GGTGCAAGCA AGCATACCAC 
AGCAAGGTGT CCTGCTTCAT GCTGCAGCAG ATCGAGGAGC CGTGCTCCCT GGGGGTCCAC 
GCCGCTGTGG TCATCCCCCC CACCTGGATC CTCCGGGCCC GCAGGCCCCA GAACACCCTC 
AAAGCCAGCA AGAAGAAAAA AAGAGCATCC TTCAAGAGGA AGTCTAGCAA GAAAGGGCCT 
GAGGAGGGCC GCTGGAGACC CTTCATCATC AGGCCTACCC CGTCCCCCCT CATGAAGCCC 
CTGCTGGTGT TCGTGAACCC CAAGAGTGGG GGCAACCAGG GCGCCAAGAT CATCCAGTCC 
TTCCTCTGGT ATCTGAATCC CCGGCAAGTC TTTGACCTGA GCCAGGGGGG CCCCAGGGAG 
GCGCTGGAGA TGTATCGCCG AGTGCACAAC CTGCGGATCC TGGCCTGCGG GGGTGACGGC 
ACGGTCGGCT GGATCCTCTC CACGCTGGAC CAGCTGCGCT TGAAGCCGCC GCCGCCAGTC 
GCCATCCTGC CCCTGGGCAC TGGCAATGAC TTGGCCCGCA CCCTCAACTG GGGCGGGGGC 
TACACTGACG AGCCTGTGTC CAAGATCCTG TCCCACGTGG AGGAGGGCAA CGTGGTACAG 
CTGGACCGCT GGGACCTCCA TGCGGAGCCC AACCCCGAGG CGGGGCCCGA GGAGCGAGAT 
GAGGGGGCCA CCGACCAGCT GCCTCTGGAT GTCTTCAACA ACTACTTCAG CCTGGGCTTT 
GACGCCCACG TCACCCTGGA GTTCCACGAG TCTCGAGAGG CCAACCCGGA GAAGTTCAAC 
AGCCGCTTCC GGAATAAGAT GTTCTACGCC GGGACAGCCT TCTCTGACTT CCTGATGGGC 
AGCTCCAAGG ACTTGGCCAA GCACATCCGC GTGGTGTGTG ATGGGACTGA CCTGACCCCC 
AAGATTCAGG ACCTGAAACC CCAGTGCATT GTTTTCTTGA ACATCCCCAG GTACTGCGCG 
GGCACCATGC CCTGGGGCCA CCCTGGGGAG CACCATGACT TTGAGCCCCA GCGGCACGAC 
GATGGCTACC TCGAGGTCAT CGGCTTTACC ATGACCTCCC TGGCTGCGCT GCAGGTGGGC 
GGGCACGGCG AGCGGCTGAC GCAGTGCCGA GAGGTGCTGC TCACCACGTC CAAAGCCATC 
CCGGTGCAGG TGGACGGTGA GCCCTGCAAG CTCGCAGCCT CGCGCATCCG CATTGCCCTG 
CGCAACCAGG CCACCATGGT GCAGAAGGCC AAGCGGCGGA GCGCCGCCCC CCTGCACAGC 
GACCAGCAGC CGGTGCCGGA GCAGCTGCGA GTCCAGGTGA GCAGGGTCAG CATGCACGAC 
TACGAGGCCC TGCACTACGA CAAGGAGCAG CTCAAAGAGG CTTCTGTGCC GCTGGGCACT 
GTGGTGGTCC CAGGAGACAG CGACCTGGAG CTGTGCCGCG CTCACATCGA GAGGCTCCGG 
CAGGAGCCCG AAGGTGCTGG AGCCAAGTCC CCGATGTGCC AGAAACTGTC CCCCAAGTGG 
TGCTTCCTCG ATGCCACCAC TGCCAGCCGC TTCTACAGAA TCGACAGGGC CCAGGAACAC 
CTCAACTACG TGACCGAGAT CGCACAGGAC GAGATTTATA TCCTGGACCC TGAGCTGCTG 
GGGGCATCTG CCCGTCCTGA CCTCCCCACC CCCACGTCCC CTCTCCCCAC CTCGCCCTGC 
TCCCCCACAT CCCGGTCACT GCCAGGGGAC GCTGCGCCCC CTACAGGTGA AGAGCTCATC 
GAGGCTGCAA AGAGGAACGA TTTCTGTAAG CTCCAGGAGC TGCACCGAGC TGGGGGTGAC 
CTTATGCACC GTGATGAGCG GAGCCGCACG CTCCTGCACC ACGCGGTCAG CACCGGCAGC 
AAGGAAGTGG TCCGCTACCT GCTGGAGCAC GCGCCCACTG AGATCCTTGA TGCCGTGGAG 
GAAAACGGGG AGACCTGCCT GCACCAGGCG GCAGCCCTGG GCCAGCGCAC CATCTGCCAC 
TACATCGTGG AGGCCGGGGC CTCGCTCATG AAGACTGACC AGCAGGGTGA CACTCCCCGG 
CAGAGAGCGG AGAAGGCTCA GGACACGGAA CTGGCGGCGT ACCTGGAAAA CCGGCAGCAT 
TACCAGATGA TCCAGCGGGA GGACCAGGAG ACAGCGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975198.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830966.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa222730
Clone ID Related Accession (Same CDS sequence)	XM_024975198.1
Accession Version	XM_024975198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGCAGA AATCAGTGTC CCGGAGAAAG TGTGCAGCCT GCAAGATTGT GGTCCACACA 
CCCTGCATTG AGCAGCTCGA GAAGATAAAT TTCCGCTGTA AGCCATCCTT CCGTGAATCG 
GGCTCCAGGA ACGTCCGTGA GCCAACCTTC GTGCGGCACC ACTGGGTACA CCGGCGACGC 
CAGGACGGCA AGTGTCGGCA CTGCGGGAAG GGCTTCCAGC AGAAGTTCAC CTTCCACAGC 
AAGGAGATCG TGGCCATCAG CTGCTCCTGG TGCAAGCAAG CATACCACAG CAAGGTGTCC 
TGCTTCATGC TGCAGCAGAT CGAGGAGCCG TGCTCCCTGG GGGTCCACGC CGCTGTGGTC 
ATCCCCCCCA CCTGGATCCT CCGGGCCCGC AGGCCCCAGA ACACCCTCAA AGCCAGCAAG 
AAGAAAAAAA GAGCATCCTT CAAGAGGAAG TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC 
TGGAGACCCT TCATCATCAG GCCTACCCCG TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC 
GTGAACCCCA AGAGTGGGGG CAACCAGGGC GCCAAGATCA TCCAGTCCTT CCTCTGGTAT 
CTGAATCCCC GGCAAGTCTT TGACCTGAGC CAGGGGGGCC CCAGGGAGGC GCTGGAGATG 
TATCGCCGAG TGCACAACCT GCGGATCCTG GCCTGCGGGG GTGACGGCAC GGTCGGCTGG 
ATCCTCTCCA CGCTGGACCA GCTGCGCTTG AAGCCGCCGC CGCCAGTCGC CATCCTGCCC 
CTGGGCACTG GCAATGACTT GGCCCGCACC CTCAACTGGG GCGGGGGCTA CACTGACGAG 
CCTGTGTCCA AGATCCTGTC CCACGTGGAG GAGGGCAACG TGGTACAGCT GGACCGCTGG 
GACCTCCATG CGGAGCCCAA CCCCGAGGCG GGGCCCGAGG AGCGAGATGA GGGGGCCACC 
GACCAGCTGC CTCTGGATGT CTTCAACAAC TACTTCAGCC TGGGCTTTGA CGCCCACGTC 
ACCCTGGAGT TCCACGAGTC TCGAGAGGCC AACCCGGAGA AGTTCAACAG CCGCTTCCGG 
AATAAGATGT TCTACGCCGG GACAGCCTTC TCTGACTTCC TGATGGGCAG CTCCAAGGAC 
TTGGCCAAGC ACATCCGCGT GGTGTGTGAT GGGACTGACC TGACCCCCAA GATTCAGGAC 
CTGAAACCCC AGTGCATTGT TTTCTTGAAC ATCCCCAGGT ACTGCGCGGG CACCATGCCC 
TGGGGCCACC CTGGGGAGCA CCATGACTTT GAGCCCCAGC GGCACGACGA TGGCTACCTC 
GAGGTCATCG GCTTTACCAT GACCTCCCTG GCTGCGCTGC AGGTGGGCGG GCACGGCGAG 
CGGCTGACGC AGTGCCGAGA GGTGCTGCTC ACCACGTCCA AAGCCATCCC GGTGCAGGTG 
GACGGTGAGC CCTGCAAGCT CGCAGCCTCG CGCATCCGCA TTGCCCTGCG CAACCAGGCC 
ACCATGGTGC AGAAGGCCAA GCGGCGGAGC GCCGCCCCCC TGCACAGCGA CCAGCAGCCG 
GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC AGGGTCAGCA TGCACGACTA CGAGGCCCTG 
CACTACGACA AGGAGCAGCT CAAAGAGGCT TCTGTGCCGC TGGGCACTGT GGTGGTCCCA 
GGAGACAGCG ACCTGGAGCT GTGCCGCGCT CACATCGAGA GGCTCCGGCA GGAGCCCGAA 
GGTGCTGGAG CCAAGTCCCC GATGTGCCAG AAACTGTCCC CCAAGTGGTG CTTCCTCGAT 
GCCACCACTG CCAGCCGCTT CTACAGAATC GACAGGGCCC AGGAACACCT CAACTACGTG 
ACCGAGATCG CACAGGACGA GATTTATATC CTGGACCCTG AGCTGCTGGG GGCATCTGCC 
CGTCCTGACC TCCCCACCCC CACGTCCCCT CTCCCCACCT CGCCCTGCTC CCCCACATCC 
CGGTCACTGC CAGGGGACGC TGCGCCCCCT ACAGGTGAAG AGCTCATCGA GGCTGCAAAG 
AGGAACGATT TCTGTAAGCT CCAGGAGCTG CACCGAGCTG GGGGTGACCT TATGCACCGT 
GATGAGCGGA GCCGCACGCT CCTGCACCAC GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC 
CGCTACCTGC TGGAGCACGC GCCCACTGAG ATCCTTGATG CCGTGGAGGA AAACGGGGAG 
ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC CAGCGCACCA TCTGCCACTA CATCGTGGAG 
GCCGGGGCCT CGCTCATGAA GACTGACCAG CAGGGTGACA CTCCCCGGCA GAGAGCGGAG 
AAGGCTCAGG ACACGGAACT GGCGGCGTAC CTGGAAAACC GGCAGCATTA CCAGATGATC 
CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830966.1
CDS	154..2943
Translation	MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLSKRRFPGLRLFGHRKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975198.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975198.1

ORF Insert Sequence:

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGCAGA AATCAGTGTC CCGGAGAAAG TGTGCAGCCT GCAAGATTGT GGTCCACACA 
CCCTGCATTG AGCAGCTCGA GAAGATAAAT TTCCGCTGTA AGCCATCCTT CCGTGAATCG 
GGCTCCAGGA ACGTCCGTGA GCCAACCTTC GTGCGGCACC ACTGGGTACA CCGGCGACGC 
CAGGACGGCA AGTGTCGGCA CTGCGGGAAG GGCTTCCAGC AGAAGTTCAC CTTCCACAGC 
AAGGAGATCG TGGCCATCAG CTGCTCCTGG TGCAAGCAAG CATACCACAG CAAGGTGTCC 
TGCTTCATGC TGCAGCAGAT CGAGGAGCCG TGCTCCCTGG GGGTCCACGC CGCTGTGGTC 
ATCCCCCCCA CCTGGATCCT CCGGGCCCGC AGGCCCCAGA ACACCCTCAA AGCCAGCAAG 
AAGAAAAAAA GAGCATCCTT CAAGAGGAAG TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC 
TGGAGACCCT TCATCATCAG GCCTACCCCG TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC 
GTGAACCCCA AGAGTGGGGG CAACCAGGGC GCCAAGATCA TCCAGTCCTT CCTCTGGTAT 
CTGAATCCCC GGCAAGTCTT TGACCTGAGC CAGGGGGGCC CCAGGGAGGC GCTGGAGATG 
TATCGCCGAG TGCACAACCT GCGGATCCTG GCCTGCGGGG GTGACGGCAC GGTCGGCTGG 
ATCCTCTCCA CGCTGGACCA GCTGCGCTTG AAGCCGCCGC CGCCAGTCGC CATCCTGCCC 
CTGGGCACTG GCAATGACTT GGCCCGCACC CTCAACTGGG GCGGGGGCTA CACTGACGAG 
CCTGTGTCCA AGATCCTGTC CCACGTGGAG GAGGGCAACG TGGTACAGCT GGACCGCTGG 
GACCTCCATG CGGAGCCCAA CCCCGAGGCG GGGCCCGAGG AGCGAGATGA GGGGGCCACC 
GACCAGCTGC CTCTGGATGT CTTCAACAAC TACTTCAGCC TGGGCTTTGA CGCCCACGTC 
ACCCTGGAGT TCCACGAGTC TCGAGAGGCC AACCCGGAGA AGTTCAACAG CCGCTTCCGG 
AATAAGATGT TCTACGCCGG GACAGCCTTC TCTGACTTCC TGATGGGCAG CTCCAAGGAC 
TTGGCCAAGC ACATCCGCGT GGTGTGTGAT GGGACTGACC TGACCCCCAA GATTCAGGAC 
CTGAAACCCC AGTGCATTGT TTTCTTGAAC ATCCCCAGGT ACTGCGCGGG CACCATGCCC 
TGGGGCCACC CTGGGGAGCA CCATGACTTT GAGCCCCAGC GGCACGACGA TGGCTACCTC 
GAGGTCATCG GCTTTACCAT GACCTCCCTG GCTGCGCTGC AGGTGGGCGG GCACGGCGAG 
CGGCTGACGC AGTGCCGAGA GGTGCTGCTC ACCACGTCCA AAGCCATCCC GGTGCAGGTG 
GACGGTGAGC CCTGCAAGCT CGCAGCCTCG CGCATCCGCA TTGCCCTGCG CAACCAGGCC 
ACCATGGTGC AGAAGGCCAA GCGGCGGAGC GCCGCCCCCC TGCACAGCGA CCAGCAGCCG 
GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC AGGGTCAGCA TGCACGACTA CGAGGCCCTG 
CACTACGACA AGGAGCAGCT CAAAGAGGCT TCTGTGCCGC TGGGCACTGT GGTGGTCCCA 
GGAGACAGCG ACCTGGAGCT GTGCCGCGCT CACATCGAGA GGCTCCGGCA GGAGCCCGAA 
GGTGCTGGAG CCAAGTCCCC GATGTGCCAG AAACTGTCCC CCAAGTGGTG CTTCCTCGAT 
GCCACCACTG CCAGCCGCTT CTACAGAATC GACAGGGCCC AGGAACACCT CAACTACGTG 
ACCGAGATCG CACAGGACGA GATTTATATC CTGGACCCTG AGCTGCTGGG GGCATCTGCC 
CGTCCTGACC TCCCCACCCC CACGTCCCCT CTCCCCACCT CGCCCTGCTC CCCCACATCC 
CGGTCACTGC CAGGGGACGC TGCGCCCCCT ACAGGTGAAG AGCTCATCGA GGCTGCAAAG 
AGGAACGATT TCTGTAAGCT CCAGGAGCTG CACCGAGCTG GGGGTGACCT TATGCACCGT 
GATGAGCGGA GCCGCACGCT CCTGCACCAC GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC 
CGCTACCTGC TGGAGCACGC GCCCACTGAG ATCCTTGATG CCGTGGAGGA AAACGGGGAG 
ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC CAGCGCACCA TCTGCCACTA CATCGTGGAG 
GCCGGGGCCT CGCTCATGAA GACTGACCAG CAGGGTGACA CTCCCCGGCA GAGAGCGGAG 
AAGGCTCAGG ACACGGAACT GGCGGCGTAC CTGGAAAACC GGCAGCATTA CCAGATGATC 
CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975199.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830967.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa222731
Clone ID Related Accession (Same CDS sequence)	XM_024975199.1
Accession Version	XM_024975199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT GCAGCCTGCA AGATTGTGGT CCACACACCC 
TGCATTGAGC AGCTCGAGAA GATAAATTTC CGCTGTAAGC CATCCTTCCG TGAATCGGGC 
TCCAGGAACG TCCGTGAGCC AACCTTCGTG CGGCACCACT GGGTACACCG GCGACGCCAG 
GACGGCAAGT GTCGGCACTG CGGGAAGGGC TTCCAGCAGA AGTTCACCTT CCACAGCAAG 
GAGATCGTGG CCATCAGCTG CTCCTGGTGC AAGCAAGCAT ACCACAGCAA GGTGTCCTGC 
TTCATGCTGC AGCAGATCGA GGAGCCGTGC TCCCTGGGGG TCCACGCCGC TGTGGTCATC 
CCCCCCACCT GGATCCTCCG GGCCCGCAGG CCCCAGAACA CCCTCAAAGC CAGCAAGAAG 
AAAAAAAGAG CATCCTTCAA GAGGAAGTCT AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG 
AGACCCTTCA TCATCAGGCC TACCCCGTCC CCCCTCATGA AGCCCCTGCT GGTGTTCGTG 
AACCCCAAGA GTGGGGGCAA CCAGGGCGCC AAGATCATCC AGTCCTTCCT CTGGTATCTG 
AATCCCCGGC AAGTCTTTGA CCTGAGCCAG GGGGGCCCCA GGGAGGCGCT GGAGATGTAT 
CGCCGAGTGC ACAACCTGCG GATCCTGGCC TGCGGGGGTG ACGGCACGGT CGGCTGGATC 
CTCTCCACGC TGGACCAGCT GCGCTTGAAG CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG 
GGCACTGGCA ATGACTTGGC CCGCACCCTC AACTGGGGCG GGGGCTACAC TGACGAGCCT 
GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG GGCAACGTGG TACAGCTGGA CCGCTGGGAC 
CTCCATGCGG AGCCCAACCC CGAGGCGGGG CCCGAGGAGC GAGATGAGGG GGCCACCGAC 
CAGCTGCCTC TGGATGTCTT CAACAACTAC TTCAGCCTGG GCTTTGACGC CCACGTCACC 
CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC CCGGAGAAGT TCAACAGCCG CTTCCGGAAT 
AAGATGTTCT ACGCCGGGAC AGCCTTCTCT GACTTCCTGA TGGGCAGCTC CAAGGACTTG 
GCCAAGCACA TCCGCGTGGT GTGTGATGGG ACTGACCTGA CCCCCAAGAT TCAGGACCTG 
AAACCCCAGT GCATTGTTTT CTTGAACATC CCCAGGTACT GCGCGGGCAC CATGCCCTGG 
GGCCACCCTG GGGAGCACCA TGACTTTGAG CCCCAGCGGC ACGACGATGG CTACCTCGAG 
GTCATCGGCT TTACCATGAC CTCCCTGGCT GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG 
CTGACGCAGT GCCGAGAGGT GCTGCTCACC ACGTCCAAAG CCATCCCGGT GCAGGTGGAC 
GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC ATCCGCATTG CCCTGCGCAA CCAGGCCACC 
ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC GCCCCCCTGC ACAGCGACCA GCAGCCGGTG 
CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG GTCAGCATGC ACGACTACGA GGCCCTGCAC 
TACGACAAGG AGCAGCTCAA AGAGGCTTCT GTGCCGCTGG GCACTGTGGT GGTCCCAGGA 
GACAGCGACC TGGAGCTGTG CCGCGCTCAC ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT 
GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA CTGTCCCCCA AGTGGTGCTT CCTCGATGCC 
ACCACTGCCA GCCGCTTCTA CAGAATCGAC AGGGCCCAGG AACACCTCAA CTACGTGACC 
GAGATCGCAC AGGACGAGAT TTATATCCTG GACCCTGAGC TGCTGGGGGC ATCTGCCCGT 
CCTGACCTCC CCACCCCCAC GTCCCCTCTC CCCACCTCGC CCTGCTCCCC CACATCCCGG 
TCACTGCCAG GGGACGCTGC GCCCCCTACA GGTGAAGAGC TCATCGAGGC TGCAAAGAGG 
AACGATTTCT GTAAGCTCCA GGAGCTGCAC CGAGCTGGGG GTGACCTTAT GCACCGTGAT 
GAGCGGAGCC GCACGCTCCT GCACCACGCG GTCAGCACCG GCAGCAAGGA AGTGGTCCGC 
TACCTGCTGG AGCACGCGCC CACTGAGATC CTTGATGCCG TGGAGGAAAA CGGGGAGACC 
TGCCTGCACC AGGCGGCAGC CCTGGGCCAG CGCACCATCT GCCACTACAT CGTGGAGGCC 
GGGGCCTCGC TCATGAAGAC TGACCAGCAG GGTGACACTC CCCGGCAGAG AGCGGAGAAG 
GCTCAGGACA CGGAACTGGC GGCGTACCTG GAAAACCGGC AGCATTACCA GATGATCCAG 
CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830967.1
CDS	1111..3537
Translation	MLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975199.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975199.1

ORF Insert Sequence:

ATGCTGAAAT CAGTGTCCCG GAGAAAGTGT GCAGCCTGCA AGATTGTGGT CCACACACCC 
TGCATTGAGC AGCTCGAGAA GATAAATTTC CGCTGTAAGC CATCCTTCCG TGAATCGGGC 
TCCAGGAACG TCCGTGAGCC AACCTTCGTG CGGCACCACT GGGTACACCG GCGACGCCAG 
GACGGCAAGT GTCGGCACTG CGGGAAGGGC TTCCAGCAGA AGTTCACCTT CCACAGCAAG 
GAGATCGTGG CCATCAGCTG CTCCTGGTGC AAGCAAGCAT ACCACAGCAA GGTGTCCTGC 
TTCATGCTGC AGCAGATCGA GGAGCCGTGC TCCCTGGGGG TCCACGCCGC TGTGGTCATC 
CCCCCCACCT GGATCCTCCG GGCCCGCAGG CCCCAGAACA CCCTCAAAGC CAGCAAGAAG 
AAAAAAAGAG CATCCTTCAA GAGGAAGTCT AGCAAGAAAG GGCCTGAGGA GGGCCGCTGG 
AGACCCTTCA TCATCAGGCC TACCCCGTCC CCCCTCATGA AGCCCCTGCT GGTGTTCGTG 
AACCCCAAGA GTGGGGGCAA CCAGGGCGCC AAGATCATCC AGTCCTTCCT CTGGTATCTG 
AATCCCCGGC AAGTCTTTGA CCTGAGCCAG GGGGGCCCCA GGGAGGCGCT GGAGATGTAT 
CGCCGAGTGC ACAACCTGCG GATCCTGGCC TGCGGGGGTG ACGGCACGGT CGGCTGGATC 
CTCTCCACGC TGGACCAGCT GCGCTTGAAG CCGCCGCCGC CAGTCGCCAT CCTGCCCCTG 
GGCACTGGCA ATGACTTGGC CCGCACCCTC AACTGGGGCG GGGGCTACAC TGACGAGCCT 
GTGTCCAAGA TCCTGTCCCA CGTGGAGGAG GGCAACGTGG TACAGCTGGA CCGCTGGGAC 
CTCCATGCGG AGCCCAACCC CGAGGCGGGG CCCGAGGAGC GAGATGAGGG GGCCACCGAC 
CAGCTGCCTC TGGATGTCTT CAACAACTAC TTCAGCCTGG GCTTTGACGC CCACGTCACC 
CTGGAGTTCC ACGAGTCTCG AGAGGCCAAC CCGGAGAAGT TCAACAGCCG CTTCCGGAAT 
AAGATGTTCT ACGCCGGGAC AGCCTTCTCT GACTTCCTGA TGGGCAGCTC CAAGGACTTG 
GCCAAGCACA TCCGCGTGGT GTGTGATGGG ACTGACCTGA CCCCCAAGAT TCAGGACCTG 
AAACCCCAGT GCATTGTTTT CTTGAACATC CCCAGGTACT GCGCGGGCAC CATGCCCTGG 
GGCCACCCTG GGGAGCACCA TGACTTTGAG CCCCAGCGGC ACGACGATGG CTACCTCGAG 
GTCATCGGCT TTACCATGAC CTCCCTGGCT GCGCTGCAGG TGGGCGGGCA CGGCGAGCGG 
CTGACGCAGT GCCGAGAGGT GCTGCTCACC ACGTCCAAAG CCATCCCGGT GCAGGTGGAC 
GGTGAGCCCT GCAAGCTCGC AGCCTCGCGC ATCCGCATTG CCCTGCGCAA CCAGGCCACC 
ATGGTGCAGA AGGCCAAGCG GCGGAGCGCC GCCCCCCTGC ACAGCGACCA GCAGCCGGTG 
CCGGAGCAGC TGCGAGTCCA GGTGAGCAGG GTCAGCATGC ACGACTACGA GGCCCTGCAC 
TACGACAAGG AGCAGCTCAA AGAGGCTTCT GTGCCGCTGG GCACTGTGGT GGTCCCAGGA 
GACAGCGACC TGGAGCTGTG CCGCGCTCAC ATCGAGAGGC TCCGGCAGGA GCCCGAAGGT 
GCTGGAGCCA AGTCCCCGAT GTGCCAGAAA CTGTCCCCCA AGTGGTGCTT CCTCGATGCC 
ACCACTGCCA GCCGCTTCTA CAGAATCGAC AGGGCCCAGG AACACCTCAA CTACGTGACC 
GAGATCGCAC AGGACGAGAT TTATATCCTG GACCCTGAGC TGCTGGGGGC ATCTGCCCGT 
CCTGACCTCC CCACCCCCAC GTCCCCTCTC CCCACCTCGC CCTGCTCCCC CACATCCCGG 
TCACTGCCAG GGGACGCTGC GCCCCCTACA GGTGAAGAGC TCATCGAGGC TGCAAAGAGG 
AACGATTTCT GTAAGCTCCA GGAGCTGCAC CGAGCTGGGG GTGACCTTAT GCACCGTGAT 
GAGCGGAGCC GCACGCTCCT GCACCACGCG GTCAGCACCG GCAGCAAGGA AGTGGTCCGC 
TACCTGCTGG AGCACGCGCC CACTGAGATC CTTGATGCCG TGGAGGAAAA CGGGGAGACC 
TGCCTGCACC AGGCGGCAGC CCTGGGCCAG CGCACCATCT GCCACTACAT CGTGGAGGCC 
GGGGCCTCGC TCATGAAGAC TGACCAGCAG GGTGACACTC CCCGGCAGAG AGCGGAGAAG 
GCTCAGGACA CGGAACTGGC GGCGTACCTG GAAAACCGGC AGCATTACCA GATGATCCAG 
CGGGAGGACC AGGAGACAGC GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975187.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830955.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222720
Clone ID Related Accession (Same CDS sequence)	XM_024975187.1
Accession Version	XM_024975187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GAAATCAGTG 
TCCCGGAGAA AGTGTGCAGC CTGCAAGATT GTGGTCCACA CACCCTGCAT TGAGCAGCTC 
GAGAAGATAA ATTTCCGCTG TAAGCCATCC TTCCGTGAAT CGGGCTCCAG GAACGTCCGT 
GAGCCAACCT TCGTGCGGCA CCACTGGGTA CACCGGCGAC GCCAGGACGG CAAGTGTCGG 
CACTGCGGGA AGGGCTTCCA GCAGAAGTTC ACCTTCCACA GCAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAGCA AGCATACCAC AGCAAGGTGT CCTGCTTCAT GCTGCAGCAG 
ATCGAGGAGC CGTGCTCCCT GGGGGTCCAC GCCGCTGTGG TCATCCCCCC CACCTGGATC 
CTCCGGGCCC GCAGGCCCCA GAACACCCTC AAAGCCAGCA AGAAGAAAAA AAGAGCATCC 
TTCAAGAGGA AGTCTAGCAA GAAAGGGCCT GAGGAGGGCC GCTGGAGACC CTTCATCATC 
AGGCCTACCC CGTCCCCCCT CATGAAGCCC CTGCTGGTGT TCGTGAACCC CAAGAGTGGG 
GGCAACCAGG GCGCCAAGAT CATCCAGTCC TTCCTCTGGT ATCTGAATCC CCGGCAAGTC 
TTTGACCTGA GCCAGGGGGG CCCCAGGGAG GCGCTGGAGA TGTATCGCCG AGTGCACAAC 
CTGCGGATCC TGGCCTGCGG GGGTGACGGC ACGGTCGGCT GGATCCTCTC CACGCTGGAC 
CAGCTGCGCT TGAAGCCGCC GCCGCCAGTC GCCATCCTGC CCCTGGGCAC TGGCAATGAC 
TTGGCCCGCA CCCTCAACTG GGGCGGGGGC TACACTGACG AGCCTGTGTC CAAGATCCTG 
TCCCACGTGG AGGAGGGCAA CGTGGTACAG CTGGACCGCT GGGACCTCCA TGCGGAGCCC 
AACCCCGAGG CGGGGCCCGA GGAGCGAGAT GAGGGGGCCA CCGACCAGCT GCCTCTGGAT 
GTCTTCAACA ACTACTTCAG CCTGGGCTTT GACGCCCACG TCACCCTGGA GTTCCACGAG 
TCTCGAGAGG CCAACCCGGA GAAGTTCAAC AGCCGCTTCC GGAATAAGAT GTTCTACGCC 
GGGACAGCCT TCTCTGACTT CCTGATGGGC AGCTCCAAGG ACTTGGCCAA GCACATCCGC 
GTGGTGTGTG ATGGGACTGA CCTGACCCCC AAGATTCAGG ACCTGAAACC CCAGTGCATT 
GTTTTCTTGA ACATCCCCAG GTACTGCGCG GGCACCATGC CCTGGGGCCA CCCTGGGGAG 
CACCATGACT TTGAGCCCCA GCGGCACGAC GATGGCTACC TCGAGGTCAT CGGCTTTACC 
ATGACCTCCC TGGCTGCGCT GCAGGTGGGC GGGCACGGCG AGCGGCTGAC GCAGTGCCGA 
GAGGTGCTGC TCACCACGTC CAAAGCCATC CCGGTGCAGG TGGACGGTGA GCCCTGCAAG 
CTCGCAGCCT CGCGCATCCG CATTGCCCTG CGCAACCAGG CCACCATGGT GCAGAAGGCC 
AAGCGGCGGA GCGCCGCCCC CCTGCACAGC GACCAGCAGC CGGTGCCGGA GCAGCTGCGA 
GTCCAGGTGA GCAGGGTCAG CATGCACGAC TACGAGGCCC TGCACTACGA CAAGGAGCAG 
CTCAAAGAGG CTTCTGTGCC GCTGGGCACT GTGGTGGTCC CAGGAGACAG CGACCTGGAG 
CTGTGCCGCG CTCACATCGA GAGGCTCCGG CAGGAGCCCG AAGGTGCTGG AGCCAAGTCC 
CCGATGTGCC AGAAACTGTC CCCCAAGTGG TGCTTCCTCG ATGCCACCAC TGCCAGCCGC 
TTCTACAGAA TCGACAGGGC CCAGGAACAC CTCAACTACG TGACCGAGAT CGCACAGGAC 
GAGATTTATA TCCTGGACCC TGAGCTGCTG GGGGCATCTG CCCGTCCTGA CCTCCCCACC 
CCCACGTCCC CTCTCCCCAC CTCGCCCTGC TCCCCCACAT CCCGGTCACT GCCAGGGGAC 
GCTGCGCCCC CTACAGGTGA AGAGCTCATC GAGGCTGCAA AGAGGAACGA TTTCTGTAAG 
CTCCAGGAGC TGCACCGAGC TGGGGGTGAC CTTATGCACC GTGATGAGCG GAGCCGCACG 
CTCCTGCACC ACGCGGTCAG CACCGGCAGC AAGGAAGTGG TCCGCTACCT GCTGGAGCAC 
GCGCCCACTG AGATCCTTGA TGCCGTGGAG GAAAACGGGG AGACCTGCCT GCACCAGGCG 
GCAGCCCTGG GCCAGCGCAC CATCTGCCAC TACATCGTGG AGGCCGGGGC CTCGCTCATG 
AAGACTGACC AGCAGGGTGA CACTCCCCGG CAGAGAGCGG AGAAGGCTCA GGACACGGAA 
CTGGCGGCGT ACCTGGAAAA CCGGCAGCAT TACCAGATGA TCCAGCGGGA GGACCAGGAG 
ACAGCGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830955.1
CDS	123..3494
Translation	METFIKRHFQRKAPGPGEGPRRPSGVGLPTSKARRRSPAGQASSSLAQRRRSSAQLQGCLLGCGVGAPRPGRRRRSSTAPPACNPRFAVEEVPTQPQPAVVGPQLLVAPLLLAGLVGMEEEEGLQKEDVRVALPSAAQPGSGTPGPSPPPPRSALPLLPVPRWRRRRASSQLLPADVVKDHGLWGLHGHYRRLSQPRPVSQNPTPGGRRASGTAAGLGMPARVRPLCRRRQVALRRKSAGPQTWSALLAKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975187.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975187.1

ORF Insert Sequence:

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GAAATCAGTG 
TCCCGGAGAA AGTGTGCAGC CTGCAAGATT GTGGTCCACA CACCCTGCAT TGAGCAGCTC 
GAGAAGATAA ATTTCCGCTG TAAGCCATCC TTCCGTGAAT CGGGCTCCAG GAACGTCCGT 
GAGCCAACCT TCGTGCGGCA CCACTGGGTA CACCGGCGAC GCCAGGACGG CAAGTGTCGG 
CACTGCGGGA AGGGCTTCCA GCAGAAGTTC ACCTTCCACA GCAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAGCA AGCATACCAC AGCAAGGTGT CCTGCTTCAT GCTGCAGCAG 
ATCGAGGAGC CGTGCTCCCT GGGGGTCCAC GCCGCTGTGG TCATCCCCCC CACCTGGATC 
CTCCGGGCCC GCAGGCCCCA GAACACCCTC AAAGCCAGCA AGAAGAAAAA AAGAGCATCC 
TTCAAGAGGA AGTCTAGCAA GAAAGGGCCT GAGGAGGGCC GCTGGAGACC CTTCATCATC 
AGGCCTACCC CGTCCCCCCT CATGAAGCCC CTGCTGGTGT TCGTGAACCC CAAGAGTGGG 
GGCAACCAGG GCGCCAAGAT CATCCAGTCC TTCCTCTGGT ATCTGAATCC CCGGCAAGTC 
TTTGACCTGA GCCAGGGGGG CCCCAGGGAG GCGCTGGAGA TGTATCGCCG AGTGCACAAC 
CTGCGGATCC TGGCCTGCGG GGGTGACGGC ACGGTCGGCT GGATCCTCTC CACGCTGGAC 
CAGCTGCGCT TGAAGCCGCC GCCGCCAGTC GCCATCCTGC CCCTGGGCAC TGGCAATGAC 
TTGGCCCGCA CCCTCAACTG GGGCGGGGGC TACACTGACG AGCCTGTGTC CAAGATCCTG 
TCCCACGTGG AGGAGGGCAA CGTGGTACAG CTGGACCGCT GGGACCTCCA TGCGGAGCCC 
AACCCCGAGG CGGGGCCCGA GGAGCGAGAT GAGGGGGCCA CCGACCAGCT GCCTCTGGAT 
GTCTTCAACA ACTACTTCAG CCTGGGCTTT GACGCCCACG TCACCCTGGA GTTCCACGAG 
TCTCGAGAGG CCAACCCGGA GAAGTTCAAC AGCCGCTTCC GGAATAAGAT GTTCTACGCC 
GGGACAGCCT TCTCTGACTT CCTGATGGGC AGCTCCAAGG ACTTGGCCAA GCACATCCGC 
GTGGTGTGTG ATGGGACTGA CCTGACCCCC AAGATTCAGG ACCTGAAACC CCAGTGCATT 
GTTTTCTTGA ACATCCCCAG GTACTGCGCG GGCACCATGC CCTGGGGCCA CCCTGGGGAG 
CACCATGACT TTGAGCCCCA GCGGCACGAC GATGGCTACC TCGAGGTCAT CGGCTTTACC 
ATGACCTCCC TGGCTGCGCT GCAGGTGGGC GGGCACGGCG AGCGGCTGAC GCAGTGCCGA 
GAGGTGCTGC TCACCACGTC CAAAGCCATC CCGGTGCAGG TGGACGGTGA GCCCTGCAAG 
CTCGCAGCCT CGCGCATCCG CATTGCCCTG CGCAACCAGG CCACCATGGT GCAGAAGGCC 
AAGCGGCGGA GCGCCGCCCC CCTGCACAGC GACCAGCAGC CGGTGCCGGA GCAGCTGCGA 
GTCCAGGTGA GCAGGGTCAG CATGCACGAC TACGAGGCCC TGCACTACGA CAAGGAGCAG 
CTCAAAGAGG CTTCTGTGCC GCTGGGCACT GTGGTGGTCC CAGGAGACAG CGACCTGGAG 
CTGTGCCGCG CTCACATCGA GAGGCTCCGG CAGGAGCCCG AAGGTGCTGG AGCCAAGTCC 
CCGATGTGCC AGAAACTGTC CCCCAAGTGG TGCTTCCTCG ATGCCACCAC TGCCAGCCGC 
TTCTACAGAA TCGACAGGGC CCAGGAACAC CTCAACTACG TGACCGAGAT CGCACAGGAC 
GAGATTTATA TCCTGGACCC TGAGCTGCTG GGGGCATCTG CCCGTCCTGA CCTCCCCACC 
CCCACGTCCC CTCTCCCCAC CTCGCCCTGC TCCCCCACAT CCCGGTCACT GCCAGGGGAC 
GCTGCGCCCC CTACAGGTGA AGAGCTCATC GAGGCTGCAA AGAGGAACGA TTTCTGTAAG 
CTCCAGGAGC TGCACCGAGC TGGGGGTGAC CTTATGCACC GTGATGAGCG GAGCCGCACG 
CTCCTGCACC ACGCGGTCAG CACCGGCAGC AAGGAAGTGG TCCGCTACCT GCTGGAGCAC 
GCGCCCACTG AGATCCTTGA TGCCGTGGAG GAAAACGGGG AGACCTGCCT GCACCAGGCG 
GCAGCCCTGG GCCAGCGCAC CATCTGCCAC TACATCGTGG AGGCCGGGGC CTCGCTCATG 
AAGACTGACC AGCAGGGTGA CACTCCCCGG CAGAGAGCGG AGAAGGCTCA GGACACGGAA 
CTGGCGGCGT ACCTGGAAAA CCGGCAGCAT TACCAGATGA TCCAGCGGGA GGACCAGGAG 
ACAGCGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975188.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830956.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X2, mRNA.

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CloneID	OBa222721
Clone ID Related Accession (Same CDS sequence)	XM_024975188.1
Accession Version	XM_024975188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCACCAC 
CCGCCTGCCC CTGCCATGGA GACCTTCATT AAGAGACACT TCCAGCGGAA GGCGCCTGGC 
CCAGGGGAGG GGCCGAGGCG GCCCAGCGGT GTGGGGCTGC CCACAAGCAA GGCCCGGCGC 
CGCTCGCCCG CGGGGCAGGC CTCCTCCTCG CTGGCACAGC GGCGGCGCTC TAGCGCGCAG 
CTCCAGGGCT GTCTCCTGGG CTGTGGGGTA GGGGCCCCGC GCCCCGGCCG CCGCCGGCGC 
TCCAGCACCG CGCCCCCCGC CTGCAATCCC CGCTTTGCCG TGGAGGAGGT GCCCACCCAG 
CCGCAGCCAG CCGTGGTGGG GCCCCAGCTT TTGGTGGCAC CCCTGCTGTT GGCTGGGCTC 
GTGGGCATGG AAGAGGAGGA GGGCTTGCAG AAGGAGGATG TGAGGGTCGC GTTGCCGAGT 
GCCGCCCAGC CGGGCTCCGG GACCCCAGGG CCATCCCCAC CGCCGCCCCG CAGCGCGCTG 
CCCCTGCTGC CCGTTCCGCG CTGGCGCCGG CGCCGGGCCT CCTCCCAGCT GCTGCCCGCA 
GACGTGGTGA AAGACCATGG GCTCTGGGGC CTGCACGGTC ACTACCGACG CCTCAGCCAG 
CCGCGGCCCG TGAGCCAGAA CCCCACCCCA GGGGGTCGAA GAGCCTCGGG TACCGCAGCC 
GGCCTCGGGA TGCCCGCCCG CGTGCGCCCG CTGTGCCGCC GGCGGCAGGT AGCCCTGCGG 
CGCAAGTCAG CCGGACCCCA GACCTGGAGT GCCCTGCTTG CGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGCAGA AATCAGTGTC CCGGAGAAAG TGTGCAGCCT GCAAGATTGT GGTCCACACA 
CCCTGCATTG AGCAGCTCGA GAAGATAAAT TTCCGCTGTA AGCCATCCTT CCGTGAATCG 
GGCTCCAGGA ACGTCCGTGA GCCAACCTTC GTGCGGCACC ACTGGGTACA CCGGCGACGC 
CAGGACGGCA AGTGTCGGCA CTGCGGGAAG GGCTTCCAGC AGAAGTTCAC CTTCCACAGC 
AAGGAGATCG TGGCCATCAG CTGCTCCTGG TGCAAGCAAG CATACCACAG CAAGGTGTCC 
TGCTTCATGC TGCAGCAGAT CGAGGAGCCG TGCTCCCTGG GGGTCCACGC CGCTGTGGTC 
ATCCCCCCCA CCTGGATCCT CCGGGCCCGC AGGCCCCAGA ACACCCTCAA AGCCAGCAAG 
AAGAAAAAAA GAGCATCCTT CAAGAGGAAG TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC 
TGGAGACCCT TCATCATCAG GCCTACCCCG TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC 
GTGAACCCCA AGAGTGGGGG CAACCAGGGC GCCAAGATCA TCCAGTCCTT CCTCTGGTAT 
CTGAATCCCC GGCAAGTCTT TGACCTGAGC CAGGGGGGCC CCAGGGAGGC GCTGGAGATG 
TATCGCCGAG TGCACAACCT GCGGATCCTG GCCTGCGGGG GTGACGGCAC GGTCGGCTGG 
ATCCTCTCCA CGCTGGACCA GCTGCGCTTG AAGCCGCCGC CGCCAGTCGC CATCCTGCCC 
CTGGGCACTG GCAATGACTT GGCCCGCACC CTCAACTGGG GCGGGGGCTA CACTGACGAG 
CCTGTGTCCA AGATCCTGTC CCACGTGGAG GAGGGCAACG TGGTACAGCT GGACCGCTGG 
GACCTCCATG CGGAGCCCAA CCCCGAGGCG GGGCCCGAGG AGCGAGATGA GGGGGCCACC 
GACCAGCTGC CTCTGGATGT CTTCAACAAC TACTTCAGCC TGGGCTTTGA CGCCCACGTC 
ACCCTGGAGT TCCACGAGTC TCGAGAGGCC AACCCGGAGA AGTTCAACAG CCGCTTCCGG 
AATAAGATGT TCTACGCCGG GACAGCCTTC TCTGACTTCC TGATGGGCAG CTCCAAGGAC 
TTGGCCAAGC ACATCCGCGT GGTGTGTGAT GGGACTGACC TGACCCCCAA GATTCAGGAC 
CTGAAACCCC AGTGCATTGT TTTCTTGAAC ATCCCCAGGT ACTGCGCGGG CACCATGCCC 
TGGGGCCACC CTGGGGAGCA CCATGACTTT GAGCCCCAGC GGCACGACGA TGGCTACCTC 
GAGGTCATCG GCTTTACCAT GACCTCCCTG GCTGCGCTGC AGGTGGGCGG GCACGGCGAG 
CGGCTGACGC AGTGCCGAGA GGTGCTGCTC ACCACGTCCA AAGCCATCCC GGTGCAGGTG 
GACGGTGAGC CCTGCAAGCT CGCAGCCTCG CGCATCCGCA TTGCCCTGCG CAACCAGGCC 
ACCATGGTGC AGAAGGCCAA GCGGCGGAGC GCCGCCCCCC TGCACAGCGA CCAGCAGCCG 
GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC AGGGTCAGCA TGCACGACTA CGAGGCCCTG 
CACTACGACA AGGAGCAGCT CAAAGAGGCT TCTGTGCCGC TGGGCACTGT GGTGGTCCCA 
GGAGACAGCG ACCTGGAGCT GTGCCGCGCT CACATCGAGA GGCTCCGGCA GGAGCCCGAA 
GGTGCTGGAG CCAAGTCCCC GATGTGCCAG AAACTGTCCC CCAAGTGGTG CTTCCTCGAT 
GCCACCACTG CCAGCCGCTT CTACAGAATC GACAGGGCCC AGGAACACCT CAACTACGTG 
ACCGAGATCG CACAGGACGA GATTTATATC CTGGACCCTG AGCTGCTGGG GGCATCTGCC 
CGTCCTGACC TCCCCACCCC CACGTCCCCT CTCCCCACCT CGCCCTGCTC CCCCACATCC 
CGGTCACTGC CAGGGGACGC TGCGCCCCCT ACAGGTGAAG AGCTCATCGA GGCTGCAAAG 
AGGAACGATT TCTGTAAGCT CCAGGAGCTG CACCGAGCTG GGGGTGACCT TATGCACCGT 
GATGAGCGGA GCCGCACGCT CCTGCACCAC GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC 
CGCTACCTGC TGGAGCACGC GCCCACTGAG ATCCTTGATG CCGTGGAGGA AAACGGGGAG 
ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC CAGCGCACCA TCTGCCACTA CATCGTGGAG 
GCCGGGGCCT CGCTCATGAA GACTGACCAG CAGGGTGACA CTCCCCGGCA GAGAGCGGAG 
AAGGCTCAGG ACACGGAACT GGCGGCGTAC CTGGAAAACC GGCAGCATTA CCAGATGATC 
CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830956.1
CDS	16..3585
Translation	MSARGAGRSTGGGCDEAAALGPAELLATEEGERPGALRQMWRYRSWDVPQIPAEVPQSHHPPAPAMETFIKRHFQRKAPGPGEGPRRPSGVGLPTSKARRRSPAGQASSSLAQRRRSSAQLQGCLLGCGVGAPRPGRRRRSSTAPPACNPRFAVEEVPTQPQPAVVGPQLLVAPLLLAGLVGMEEEEGLQKEDVRVALPSAAQPGSGTPGPSPPPPRSALPLLPVPRWRRRRASSQLLPADVVKDHGLWGLHGHYRRLSQPRPVSQNPTPGGRRASGTAAGLGMPARVRPLCRRRQVALRRKSAGPQTWSALLAKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975188.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975188.1

ORF Insert Sequence:

ATGAGCGCAC GGGGGGCCGG CCGCAGTACT GGGGGAGGCT GCGATGAGGC TGCAGCTCTG 
GGCCCCGCGG AGCTTCTGGC GACCGAAGAA GGGGAGCGGC CCGGGGCTCT GAGACAGATG 
TGGCGCTACC GCTCCTGGGA CGTCCCACAG ATCCCAGCCG AGGTCCCCCA GTCTCACCAC 
CCGCCTGCCC CTGCCATGGA GACCTTCATT AAGAGACACT TCCAGCGGAA GGCGCCTGGC 
CCAGGGGAGG GGCCGAGGCG GCCCAGCGGT GTGGGGCTGC CCACAAGCAA GGCCCGGCGC 
CGCTCGCCCG CGGGGCAGGC CTCCTCCTCG CTGGCACAGC GGCGGCGCTC TAGCGCGCAG 
CTCCAGGGCT GTCTCCTGGG CTGTGGGGTA GGGGCCCCGC GCCCCGGCCG CCGCCGGCGC 
TCCAGCACCG CGCCCCCCGC CTGCAATCCC CGCTTTGCCG TGGAGGAGGT GCCCACCCAG 
CCGCAGCCAG CCGTGGTGGG GCCCCAGCTT TTGGTGGCAC CCCTGCTGTT GGCTGGGCTC 
GTGGGCATGG AAGAGGAGGA GGGCTTGCAG AAGGAGGATG TGAGGGTCGC GTTGCCGAGT 
GCCGCCCAGC CGGGCTCCGG GACCCCAGGG CCATCCCCAC CGCCGCCCCG CAGCGCGCTG 
CCCCTGCTGC CCGTTCCGCG CTGGCGCCGG CGCCGGGCCT CCTCCCAGCT GCTGCCCGCA 
GACGTGGTGA AAGACCATGG GCTCTGGGGC CTGCACGGTC ACTACCGACG CCTCAGCCAG 
CCGCGGCCCG TGAGCCAGAA CCCCACCCCA GGGGGTCGAA GAGCCTCGGG TACCGCAGCC 
GGCCTCGGGA TGCCCGCCCG CGTGCGCCCG CTGTGCCGCC GGCGGCAGGT AGCCCTGCGG 
CGCAAGTCAG CCGGACCCCA GACCTGGAGT GCCCTGCTTG CGAAAGCCAT CACCAAGTCG 
GGCCTCCAGC ACCTGGCACC CCCTCCTCCT GCTCCTGGGG CCCCGTGCAG CGAGCCTGAG 
CGGCAGATCC GGAGCACCGT GGACTGGAGT GAGTCAGCGA CGTATGGGGA ACACATCTGG 
TTTGAGACCA ACGTGTCCGG GGACTTCTGC TACGTCGGAG AGCAGTACTG CGTCGCCAAG 
ATGCTGCAGA AATCAGTGTC CCGGAGAAAG TGTGCAGCCT GCAAGATTGT GGTCCACACA 
CCCTGCATTG AGCAGCTCGA GAAGATAAAT TTCCGCTGTA AGCCATCCTT CCGTGAATCG 
GGCTCCAGGA ACGTCCGTGA GCCAACCTTC GTGCGGCACC ACTGGGTACA CCGGCGACGC 
CAGGACGGCA AGTGTCGGCA CTGCGGGAAG GGCTTCCAGC AGAAGTTCAC CTTCCACAGC 
AAGGAGATCG TGGCCATCAG CTGCTCCTGG TGCAAGCAAG CATACCACAG CAAGGTGTCC 
TGCTTCATGC TGCAGCAGAT CGAGGAGCCG TGCTCCCTGG GGGTCCACGC CGCTGTGGTC 
ATCCCCCCCA CCTGGATCCT CCGGGCCCGC AGGCCCCAGA ACACCCTCAA AGCCAGCAAG 
AAGAAAAAAA GAGCATCCTT CAAGAGGAAG TCTAGCAAGA AAGGGCCTGA GGAGGGCCGC 
TGGAGACCCT TCATCATCAG GCCTACCCCG TCCCCCCTCA TGAAGCCCCT GCTGGTGTTC 
GTGAACCCCA AGAGTGGGGG CAACCAGGGC GCCAAGATCA TCCAGTCCTT CCTCTGGTAT 
CTGAATCCCC GGCAAGTCTT TGACCTGAGC CAGGGGGGCC CCAGGGAGGC GCTGGAGATG 
TATCGCCGAG TGCACAACCT GCGGATCCTG GCCTGCGGGG GTGACGGCAC GGTCGGCTGG 
ATCCTCTCCA CGCTGGACCA GCTGCGCTTG AAGCCGCCGC CGCCAGTCGC CATCCTGCCC 
CTGGGCACTG GCAATGACTT GGCCCGCACC CTCAACTGGG GCGGGGGCTA CACTGACGAG 
CCTGTGTCCA AGATCCTGTC CCACGTGGAG GAGGGCAACG TGGTACAGCT GGACCGCTGG 
GACCTCCATG CGGAGCCCAA CCCCGAGGCG GGGCCCGAGG AGCGAGATGA GGGGGCCACC 
GACCAGCTGC CTCTGGATGT CTTCAACAAC TACTTCAGCC TGGGCTTTGA CGCCCACGTC 
ACCCTGGAGT TCCACGAGTC TCGAGAGGCC AACCCGGAGA AGTTCAACAG CCGCTTCCGG 
AATAAGATGT TCTACGCCGG GACAGCCTTC TCTGACTTCC TGATGGGCAG CTCCAAGGAC 
TTGGCCAAGC ACATCCGCGT GGTGTGTGAT GGGACTGACC TGACCCCCAA GATTCAGGAC 
CTGAAACCCC AGTGCATTGT TTTCTTGAAC ATCCCCAGGT ACTGCGCGGG CACCATGCCC 
TGGGGCCACC CTGGGGAGCA CCATGACTTT GAGCCCCAGC GGCACGACGA TGGCTACCTC 
GAGGTCATCG GCTTTACCAT GACCTCCCTG GCTGCGCTGC AGGTGGGCGG GCACGGCGAG 
CGGCTGACGC AGTGCCGAGA GGTGCTGCTC ACCACGTCCA AAGCCATCCC GGTGCAGGTG 
GACGGTGAGC CCTGCAAGCT CGCAGCCTCG CGCATCCGCA TTGCCCTGCG CAACCAGGCC 
ACCATGGTGC AGAAGGCCAA GCGGCGGAGC GCCGCCCCCC TGCACAGCGA CCAGCAGCCG 
GTGCCGGAGC AGCTGCGAGT CCAGGTGAGC AGGGTCAGCA TGCACGACTA CGAGGCCCTG 
CACTACGACA AGGAGCAGCT CAAAGAGGCT TCTGTGCCGC TGGGCACTGT GGTGGTCCCA 
GGAGACAGCG ACCTGGAGCT GTGCCGCGCT CACATCGAGA GGCTCCGGCA GGAGCCCGAA 
GGTGCTGGAG CCAAGTCCCC GATGTGCCAG AAACTGTCCC CCAAGTGGTG CTTCCTCGAT 
GCCACCACTG CCAGCCGCTT CTACAGAATC GACAGGGCCC AGGAACACCT CAACTACGTG 
ACCGAGATCG CACAGGACGA GATTTATATC CTGGACCCTG AGCTGCTGGG GGCATCTGCC 
CGTCCTGACC TCCCCACCCC CACGTCCCCT CTCCCCACCT CGCCCTGCTC CCCCACATCC 
CGGTCACTGC CAGGGGACGC TGCGCCCCCT ACAGGTGAAG AGCTCATCGA GGCTGCAAAG 
AGGAACGATT TCTGTAAGCT CCAGGAGCTG CACCGAGCTG GGGGTGACCT TATGCACCGT 
GATGAGCGGA GCCGCACGCT CCTGCACCAC GCGGTCAGCA CCGGCAGCAA GGAAGTGGTC 
CGCTACCTGC TGGAGCACGC GCCCACTGAG ATCCTTGATG CCGTGGAGGA AAACGGGGAG 
ACCTGCCTGC ACCAGGCGGC AGCCCTGGGC CAGCGCACCA TCTGCCACTA CATCGTGGAG 
GCCGGGGCCT CGCTCATGAA GACTGACCAG CAGGGTGACA CTCCCCGGCA GAGAGCGGAG 
AAGGCTCAGG ACACGGAACT GGCGGCGTAC CTGGAAAACC GGCAGCATTA CCAGATGATC 
CAGCGGGAGG ACCAGGAGAC AGCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975189.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830957.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa222722
Clone ID Related Accession (Same CDS sequence)	XM_024975189.1
Accession Version	XM_024975189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GCCACCCGCC TGCCCCTGCC 
ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830957.1
CDS	87..3641
Translation	MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLSKRRFPGLRLFGHSHPPAPAMETFIKRHFQRKAPGPGEGPRRPSGVGLPTSKARRRSPAGQASSSLAQRRRSSAQLQGCLLGCGVGAPRPGRRRRSSTAPPACNPRFAVEEVPTQPQPAVVGPQLLVAPLLLAGLVGMEEEEGLQKEDVRVALPSAAQPGSGTPGPSPPPPRSALPLLPVPRWRRRRASSQLLPADVVKDHGLWGLHGHYRRLSQPRPVSQNPTPGGRRASGTAAGLGMPARVRPLCRRRQVALRRKSAGPQTWSALLAKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975189.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975189.1

ORF Insert Sequence:

ATGGAGCCGC GGGACGGCAG CCCCGAGGCC CGGAGCAGCG ACTCCGAGTC GGCCTCCGCC 
TCGTCCAGCG GCTCCGAGCG CGACGCGGGT CCCGAGCCGG ACAAGGCGCC GCGGCGTCTC 
AGCAAGCGGC GCTTCCCAGG GCTGCGGCTT TTCGGGCACA GCCACCCGCC TGCCCCTGCC 
ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975190.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830958.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X4, mRNA.

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CloneID	OBa222723
Clone ID Related Accession (Same CDS sequence)	XM_024975190.1 , XM_024975191.1
Accession Version	XM_024975190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830958.1
CDS	591..3965
Translation	METFIKRHFQRKAPGPGEGPRRPSGVGLPTSKARRRSPAGQASSSLAQRRRSSAQLQGCLLGCGVGAPRPGRRRRSSTAPPACNPRFAVEEVPTQPQPAVVGPQLLVAPLLLAGLVGMEEEEGLQKEDVRVALPSAAQPGSGTPGPSPPPPRSALPLLPVPRWRRRRASSQLLPADVVKDHGLWGLHGHYRRLSQPRPVSQNPTPGGRRASGTAAGLGMPARVRPLCRRRQVALRRKSAGPQTWSALLAKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975190.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975190.1

ORF Insert Sequence:

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKZ ( XM_024975191.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830959.1 Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X5, mRNA.

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CloneID	OBa222723
Clone ID Related Accession (Same CDS sequence)	XM_024975190.1 , XM_024975191.1
Accession Version	XM_024975191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase zeta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830959.1
CDS	162..3536
Translation	METFIKRHFQRKAPGPGEGPRRPSGVGLPTSKARRRSPAGQASSSLAQRRRSSAQLQGCLLGCGVGAPRPGRRRRSSTAPPACNPRFAVEEVPTQPQPAVVGPQLLVAPLLLAGLVGMEEEEGLQKEDVRVALPSAAQPGSGTPGPSPPPPRSALPLLPVPRWRRRRASSQLLPADVVKDHGLWGLHGHYRRLSQPRPVSQNPTPGGRRASGTAAGLGMPARVRPLCRRRQVALRRKSAGPQTWSALLAKAITKSGLQHLAPPPPAPGAPCSEPERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVAKMLQKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRRVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEERDEGATDQLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGTDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRVQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLRQEPEGAGAKSPMCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTSRSLPGDAAPPTGEELIEAAKRNDFCKLQELHRAGGDLMHRDERSRTLLHHAVSTGSKEVVRYLLEHAPTEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_024975191.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase zeta (DGKZ), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975191.1

ORF Insert Sequence:

ATGGAGACCT TCATTAAGAG ACACTTCCAG CGGAAGGCGC CTGGCCCAGG GGAGGGGCCG 
AGGCGGCCCA GCGGTGTGGG GCTGCCCACA AGCAAGGCCC GGCGCCGCTC GCCCGCGGGG 
CAGGCCTCCT CCTCGCTGGC ACAGCGGCGG CGCTCTAGCG CGCAGCTCCA GGGCTGTCTC 
CTGGGCTGTG GGGTAGGGGC CCCGCGCCCC GGCCGCCGCC GGCGCTCCAG CACCGCGCCC 
CCCGCCTGCA ATCCCCGCTT TGCCGTGGAG GAGGTGCCCA CCCAGCCGCA GCCAGCCGTG 
GTGGGGCCCC AGCTTTTGGT GGCACCCCTG CTGTTGGCTG GGCTCGTGGG CATGGAAGAG 
GAGGAGGGCT TGCAGAAGGA GGATGTGAGG GTCGCGTTGC CGAGTGCCGC CCAGCCGGGC 
TCCGGGACCC CAGGGCCATC CCCACCGCCG CCCCGCAGCG CGCTGCCCCT GCTGCCCGTT 
CCGCGCTGGC GCCGGCGCCG GGCCTCCTCC CAGCTGCTGC CCGCAGACGT GGTGAAAGAC 
CATGGGCTCT GGGGCCTGCA CGGTCACTAC CGACGCCTCA GCCAGCCGCG GCCCGTGAGC 
CAGAACCCCA CCCCAGGGGG TCGAAGAGCC TCGGGTACCG CAGCCGGCCT CGGGATGCCC 
GCCCGCGTGC GCCCGCTGTG CCGCCGGCGG CAGGTAGCCC TGCGGCGCAA GTCAGCCGGA 
CCCCAGACCT GGAGTGCCCT GCTTGCGAAA GCCATCACCA AGTCGGGCCT CCAGCACCTG 
GCACCCCCTC CTCCTGCTCC TGGGGCCCCG TGCAGCGAGC CTGAGCGGCA GATCCGGAGC 
ACCGTGGACT GGAGTGAGTC AGCGACGTAT GGGGAACACA TCTGGTTTGA GACCAACGTG 
TCCGGGGACT TCTGCTACGT CGGAGAGCAG TACTGCGTCG CCAAGATGCT GCAGAAATCA 
GTGTCCCGGA GAAAGTGTGC AGCCTGCAAG ATTGTGGTCC ACACACCCTG CATTGAGCAG 
CTCGAGAAGA TAAATTTCCG CTGTAAGCCA TCCTTCCGTG AATCGGGCTC CAGGAACGTC 
CGTGAGCCAA CCTTCGTGCG GCACCACTGG GTACACCGGC GACGCCAGGA CGGCAAGTGT 
CGGCACTGCG GGAAGGGCTT CCAGCAGAAG TTCACCTTCC ACAGCAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA GCAAGCATAC CACAGCAAGG TGTCCTGCTT CATGCTGCAG 
CAGATCGAGG AGCCGTGCTC CCTGGGGGTC CACGCCGCTG TGGTCATCCC CCCCACCTGG 
ATCCTCCGGG CCCGCAGGCC CCAGAACACC CTCAAAGCCA GCAAGAAGAA AAAAAGAGCA 
TCCTTCAAGA GGAAGTCTAG CAAGAAAGGG CCTGAGGAGG GCCGCTGGAG ACCCTTCATC 
ATCAGGCCTA CCCCGTCCCC CCTCATGAAG CCCCTGCTGG TGTTCGTGAA CCCCAAGAGT 
GGGGGCAACC AGGGCGCCAA GATCATCCAG TCCTTCCTCT GGTATCTGAA TCCCCGGCAA 
GTCTTTGACC TGAGCCAGGG GGGCCCCAGG GAGGCGCTGG AGATGTATCG CCGAGTGCAC 
AACCTGCGGA TCCTGGCCTG CGGGGGTGAC GGCACGGTCG GCTGGATCCT CTCCACGCTG 
GACCAGCTGC GCTTGAAGCC GCCGCCGCCA GTCGCCATCC TGCCCCTGGG CACTGGCAAT 
GACTTGGCCC GCACCCTCAA CTGGGGCGGG GGCTACACTG ACGAGCCTGT GTCCAAGATC 
CTGTCCCACG TGGAGGAGGG CAACGTGGTA CAGCTGGACC GCTGGGACCT CCATGCGGAG 
CCCAACCCCG AGGCGGGGCC CGAGGAGCGA GATGAGGGGG CCACCGACCA GCTGCCTCTG 
GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC ACGTCACCCT GGAGTTCCAC 
GAGTCTCGAG AGGCCAACCC GGAGAAGTTC AACAGCCGCT TCCGGAATAA GATGTTCTAC 
GCCGGGACAG CCTTCTCTGA CTTCCTGATG GGCAGCTCCA AGGACTTGGC CAAGCACATC 
CGCGTGGTGT GTGATGGGAC TGACCTGACC CCCAAGATTC AGGACCTGAA ACCCCAGTGC 
ATTGTTTTCT TGAACATCCC CAGGTACTGC GCGGGCACCA TGCCCTGGGG CCACCCTGGG 
GAGCACCATG ACTTTGAGCC CCAGCGGCAC GACGATGGCT ACCTCGAGGT CATCGGCTTT 
ACCATGACCT CCCTGGCTGC GCTGCAGGTG GGCGGGCACG GCGAGCGGCT GACGCAGTGC 
CGAGAGGTGC TGCTCACCAC GTCCAAAGCC ATCCCGGTGC AGGTGGACGG TGAGCCCTGC 
AAGCTCGCAG CCTCGCGCAT CCGCATTGCC CTGCGCAACC AGGCCACCAT GGTGCAGAAG 
GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC AGCCGGTGCC GGAGCAGCTG 
CGAGTCCAGG TGAGCAGGGT CAGCATGCAC GACTACGAGG CCCTGCACTA CGACAAGGAG 
CAGCTCAAAG AGGCTTCTGT GCCGCTGGGC ACTGTGGTGG TCCCAGGAGA CAGCGACCTG 
GAGCTGTGCC GCGCTCACAT CGAGAGGCTC CGGCAGGAGC CCGAAGGTGC TGGAGCCAAG 
TCCCCGATGT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC TCGATGCCAC CACTGCCAGC 
CGCTTCTACA GAATCGACAG GGCCCAGGAA CACCTCAACT ACGTGACCGA GATCGCACAG 
GACGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT CTGCCCGTCC TGACCTCCCC 
ACCCCCACGT CCCCTCTCCC CACCTCGCCC TGCTCCCCCA CATCCCGGTC ACTGCCAGGG 
GACGCTGCGC CCCCTACAGG TGAAGAGCTC ATCGAGGCTG CAAAGAGGAA CGATTTCTGT 
AAGCTCCAGG AGCTGCACCG AGCTGGGGGT GACCTTATGC ACCGTGATGA GCGGAGCCGC 
ACGCTCCTGC ACCACGCGGT CAGCACCGGC AGCAAGGAAG TGGTCCGCTA CCTGCTGGAG 
CACGCGCCCA CTGAGATCCT TGATGCCGTG GAGGAAAACG GGGAGACCTG CCTGCACCAG 
GCGGCAGCCC TGGGCCAGCG CACCATCTGC CACTACATCG TGGAGGCCGG GGCCTCGCTC 
ATGAAGACTG ACCAGCAGGG TGACACTCCC CGGCAGAGAG CGGAGAAGGC TCAGGACACG 
GAACTGGCGG CGTACCTGGA AAACCGGCAG CATTACCAGA TGATCCAGCG GGAGGACCAG 
GAGACAGCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DGKZ
Entrez Gene ID	511887
Full Name	diacylglycerol kinase zeta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	DGKZ	XP_508395.2
Mus musculus (house mouse)	Dgkz	NP_001160069.1
Gallus gallus (chicken)	DGKZ	NP_001026363.1
Homo sapiens (human)	DGKZ	NP_001099010.1
	DGKZ	XP_001102461.2
Xenopus tropicalis (tropical clawed frog)	dgkz	XP_004913437.1
Canis lupus familiaris (dog)	DGKZ	XP_850588.2
Rattus norvegicus (Norway rat)	Dgkz	NP_112405.1
Drosophila melanogaster (fruit fly)	rdgA	NP_001259366.1
Caenorhabditis elegans (roundworm)	dgk-5	NP_495301.1
Bos taurus (cattle)	DGKZ	NP_001265581.1
Danio rerio (zebrafish)	dgkza	XP_005169089.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000519	XP_310575.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DGKZ cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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