MAP3K4 cDNA ORF clone, Bos taurus(cattle)

The following MAP3K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238167 XM_015472931.2
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa238168 XM_015472932.2
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa91534 XM_015472932.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91534 XM_015464893.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91535 XM_015464894.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91535 XM_005211105.3
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa22804 XM_002690377.6
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa22806 XM_005211105.4
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91532 XM_015464891.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91532 XM_015472931.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91533 XM_002690377.5
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91533 XM_015464892.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238167
    Clone ID Related Accession (Same CDS sequence) XM_015472931.2
    Accession Version XM_015472931.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGGTTTGT ACTTTGCCAT TCATCGGAAT
    AGCCCCCGTC CTGTGAAGGT ACCTCGATGC CATAGCGACC CTCCTAACCC TCACCTGATT
    ATCCCAACTC CAGAGGGATT CAGCGCCCGG AGCGTGCCCG CGGATGTGCG GAGCCTCGGC
    AGCCCCGCGC CCGCGCCTGC CGCCGCTCGT CCTGGCCCCT CGGGCAGCGA CCCAGCACCG
    CCCAAGCCCA TCAGCGGTGC CCATGAGACC AGGGGTTCCA GCGTCCCTGA AAATGACCGC
    CTGGCTTCCA TCGCAGCTGA ATTGCAGTTC AGGTCGCTGA GCCGCCACTC CAGCCCCACC
    GAGGAGCGCG AGGAACCAGC ATATCCAAAA GGTGATTCAA GTGGGTCAAC TCGAAGAAGT
    TGGGAACTTC GGACGCTGAT CAGTCAGACC AAGGATACCG CTTCTAAGCA AGGACCCATA
    GAAGCCATTC AGAAGTCAGT CCGGCTGTTT GAAGAAAAGC GGTATCGAGA AATGAGGAGG
    AAAAACATCA TTGGTCAAGT CTGTGACACC CCCAAGTCTT ACGACAACGT CATGCACGTT
    GGCTTGAGGA AGGTGACCTT TAAGTGGCAA AGAGGAAACA AAATCGGGGA AGGGCAGTAC
    GGGAAGGTGT ACACCTGCAT CAACGTCGAC ACCGGCGAGC TGATGGCCAT GAAGGAGATT
    CGATTTCAAC CCAACGACCA TAAAACCATC AAGGAAACTG CAGACGAGCT GAAAATCTTT
    GAAGGCATCA AGCACCCCAA TCTGGTTCGG TATTTTGGTG TGGAGCTACA TAGAGAGGAG
    ATGTACATCT TCATGGAGTA CTGTGACGAG GGCACGTTGG AAGAGGTGTC GCGGCTGGGG
    CTTCAAGAAC ACGTCATCAG GCTGTATTCG AAACAGATTA CCGTGGCCAT CAACGTCCTG
    CACGAGCATG GCATAGTTCA CCGCGACGTT AAAGGTGCCA ACATCTTCCT GACCTCGTCT
    GGACTAATCA AGCTGGGGGA TTTCGGATGC TCAGTAAAGC TTAAGAACAA TGCGCAGACC
    ATGCCTGGTG AAGTGAACAG CACGCTGGGG ACAGCAGCGT ACATGGCTCC GGAAGTCATC
    ACTCGCGCCA AAGGAGAAGG CCACGGGCGT GCGGCTGACA TCTGGAGCCT GGGGTGTGTT
    GTCATAGAGA TGGTGACTGG CAAGAGACCT TGGCATGAAT ATGAACACAA CTTTCAGATT
    ATGTACAAAG TGGGAATGGG GCACAAGCCG CCGATCCCTG AACGATTAAG CCCCGAAGGG
    AAGGACTTCC TCTCTCACTG CCTGGAGAGC GAGCCCCGGA TGCGGTGGAC AGCCAGCCAG
    CTCCTCGACC ACTCCTTCGT CAAGGTCTGC ACGGATGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328417.2
    CDS51..4835
    Translation

    Target ORF information:

    RefSeq Version XM_015472931.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472931.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGGTTTGT ACTTTGCCAT TCATCGGAAT
    AGCCCCCGTC CTGTGAAGGT ACCTCGATGC CATAGCGACC CTCCTAACCC TCACCTGATT
    ATCCCAACTC CAGAGGGATT CAGCGCCCGG AGCGTGCCCG CGGATGTGCG GAGCCTCGGC
    AGCCCCGCGC CCGCGCCTGC CGCCGCTCGT CCTGGCCCCT CGGGCAGCGA CCCAGCACCG
    CCCAAGCCCA TCAGCGGTGC CCATGAGACC AGGGGTTCCA GCGTCCCTGA AAATGACCGC
    CTGGCTTCCA TCGCAGCTGA ATTGCAGTTC AGGTCGCTGA GCCGCCACTC CAGCCCCACC
    GAGGAGCGCG AGGAACCAGC ATATCCAAAA GGTGATTCAA GTGGGTCAAC TCGAAGAAGT
    TGGGAACTTC GGACGCTGAT CAGTCAGACC AAGGATACCG CTTCTAAGCA AGGACCCATA
    GAAGCCATTC AGAAGTCAGT CCGGCTGTTT GAAGAAAAGC GGTATCGAGA AATGAGGAGG
    AAAAACATCA TTGGTCAAGT CTGTGACACC CCCAAGTCTT ACGACAACGT CATGCACGTT
    GGCTTGAGGA AGGTGACCTT TAAGTGGCAA AGAGGAAACA AAATCGGGGA AGGGCAGTAC
    GGGAAGGTGT ACACCTGCAT CAACGTCGAC ACCGGCGAGC TGATGGCCAT GAAGGAGATT
    CGATTTCAAC CCAACGACCA TAAAACCATC AAGGAAACTG CAGACGAGCT GAAAATCTTT
    GAAGGCATCA AGCACCCCAA TCTGGTTCGG TATTTTGGTG TGGAGCTACA TAGAGAGGAG
    ATGTACATCT TCATGGAGTA CTGTGACGAG GGCACGTTGG AAGAGGTGTC GCGGCTGGGG
    CTTCAAGAAC ACGTCATCAG GCTGTATTCG AAACAGATTA CCGTGGCCAT CAACGTCCTG
    CACGAGCATG GCATAGTTCA CCGCGACGTT AAAGGTGCCA ACATCTTCCT GACCTCGTCT
    GGACTAATCA AGCTGGGGGA TTTCGGATGC TCAGTAAAGC TTAAGAACAA TGCGCAGACC
    ATGCCTGGTG AAGTGAACAG CACGCTGGGG ACAGCAGCGT ACATGGCTCC GGAAGTCATC
    ACTCGCGCCA AAGGAGAAGG CCACGGGCGT GCGGCTGACA TCTGGAGCCT GGGGTGTGTT
    GTCATAGAGA TGGTGACTGG CAAGAGACCT TGGCATGAAT ATGAACACAA CTTTCAGATT
    ATGTACAAAG TGGGAATGGG GCACAAGCCG CCGATCCCTG AACGATTAAG CCCCGAAGGG
    AAGGACTTCC TCTCTCACTG CCTGGAGAGC GAGCCCCGGA TGCGGTGGAC AGCCAGCCAG
    CTCCTCGACC ACTCCTTCGT CAAGGTCTGC ACGGATGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238168
    Clone ID Related Accession (Same CDS sequence) XM_015472932.2
    Accession Version XM_015472932.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGGTTTGT ACTTTGCCAT TCATCGGAAT
    AGCCCCCGTC CTGTGAAGGT ACCTCGATGC CATAGCGACC CTCCTAACCC TCACCTGATT
    ATCCCAACTC CAGAGGGATT CAGGGGTTCC AGCGTCCCTG AAAATGACCG CCTGGCTTCC
    ATCGCAGCTG AATTGCAGTT CAGGTCGCTG AGCCGCCACT CCAGCCCCAC CGAGGAGCGC
    GAGGAACCAG CATATCCAAA AGGTGATTCA AGTGGGTCAA CTCGAAGAAG TTGGGAACTT
    CGGACGCTGA TCAGTCAGAC CAAGGATACC GCTTCTAAGC AAGGACCCAT AGAAGCCATT
    CAGAAGTCAG TCCGGCTGTT TGAAGAAAAG CGGTATCGAG AAATGAGGAG GAAAAACATC
    ATTGGTCAAG TCTGTGACAC CCCCAAGTCT TACGACAACG TCATGCACGT TGGCTTGAGG
    AAGGTGACCT TTAAGTGGCA AAGAGGAAAC AAAATCGGGG AAGGGCAGTA CGGGAAGGTG
    TACACCTGCA TCAACGTCGA CACCGGCGAG CTGATGGCCA TGAAGGAGAT TCGATTTCAA
    CCCAACGACC ATAAAACCAT CAAGGAAACT GCAGACGAGC TGAAAATCTT TGAAGGCATC
    AAGCACCCCA ATCTGGTTCG GTATTTTGGT GTGGAGCTAC ATAGAGAGGA GATGTACATC
    TTCATGGAGT ACTGTGACGA GGGCACGTTG GAAGAGGTGT CGCGGCTGGG GCTTCAAGAA
    CACGTCATCA GGCTGTATTC GAAACAGATT ACCGTGGCCA TCAACGTCCT GCACGAGCAT
    GGCATAGTTC ACCGCGACGT TAAAGGTGCC AACATCTTCC TGACCTCGTC TGGACTAATC
    AAGCTGGGGG ATTTCGGATG CTCAGTAAAG CTTAAGAACA ATGCGCAGAC CATGCCTGGT
    GAAGTGAACA GCACGCTGGG GACAGCAGCG TACATGGCTC CGGAAGTCAT CACTCGCGCC
    AAAGGAGAAG GCCACGGGCG TGCGGCTGAC ATCTGGAGCC TGGGGTGTGT TGTCATAGAG
    ATGGTGACTG GCAAGAGACC TTGGCATGAA TATGAACACA ACTTTCAGAT TATGTACAAA
    GTGGGAATGG GGCACAAGCC GCCGATCCCT GAACGATTAA GCCCCGAAGG GAAGGACTTC
    CTCTCTCACT GCCTGGAGAG CGAGCCCCGG ATGCGGTGGA CAGCCAGCCA GCTCCTCGAC
    CACTCCTTCG TCAAGGTCTG CACGGATGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328418.2
    CDS51..4706
    Translation

    Target ORF information:

    RefSeq Version XM_015472932.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472932.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGGTTTGT ACTTTGCCAT TCATCGGAAT
    AGCCCCCGTC CTGTGAAGGT ACCTCGATGC CATAGCGACC CTCCTAACCC TCACCTGATT
    ATCCCAACTC CAGAGGGATT CAGGGGTTCC AGCGTCCCTG AAAATGACCG CCTGGCTTCC
    ATCGCAGCTG AATTGCAGTT CAGGTCGCTG AGCCGCCACT CCAGCCCCAC CGAGGAGCGC
    GAGGAACCAG CATATCCAAA AGGTGATTCA AGTGGGTCAA CTCGAAGAAG TTGGGAACTT
    CGGACGCTGA TCAGTCAGAC CAAGGATACC GCTTCTAAGC AAGGACCCAT AGAAGCCATT
    CAGAAGTCAG TCCGGCTGTT TGAAGAAAAG CGGTATCGAG AAATGAGGAG GAAAAACATC
    ATTGGTCAAG TCTGTGACAC CCCCAAGTCT TACGACAACG TCATGCACGT TGGCTTGAGG
    AAGGTGACCT TTAAGTGGCA AAGAGGAAAC AAAATCGGGG AAGGGCAGTA CGGGAAGGTG
    TACACCTGCA TCAACGTCGA CACCGGCGAG CTGATGGCCA TGAAGGAGAT TCGATTTCAA
    CCCAACGACC ATAAAACCAT CAAGGAAACT GCAGACGAGC TGAAAATCTT TGAAGGCATC
    AAGCACCCCA ATCTGGTTCG GTATTTTGGT GTGGAGCTAC ATAGAGAGGA GATGTACATC
    TTCATGGAGT ACTGTGACGA GGGCACGTTG GAAGAGGTGT CGCGGCTGGG GCTTCAAGAA
    CACGTCATCA GGCTGTATTC GAAACAGATT ACCGTGGCCA TCAACGTCCT GCACGAGCAT
    GGCATAGTTC ACCGCGACGT TAAAGGTGCC AACATCTTCC TGACCTCGTC TGGACTAATC
    AAGCTGGGGG ATTTCGGATG CTCAGTAAAG CTTAAGAACA ATGCGCAGAC CATGCCTGGT
    GAAGTGAACA GCACGCTGGG GACAGCAGCG TACATGGCTC CGGAAGTCAT CACTCGCGCC
    AAAGGAGAAG GCCACGGGCG TGCGGCTGAC ATCTGGAGCC TGGGGTGTGT TGTCATAGAG
    ATGGTGACTG GCAAGAGACC TTGGCATGAA TATGAACACA ACTTTCAGAT TATGTACAAA
    GTGGGAATGG GGCACAAGCC GCCGATCCCT GAACGATTAA GCCCCGAAGG GAAGGACTTC
    CTCTCTCACT GCCTGGAGAG CGAGCCCCGG ATGCGGTGGA CAGCCAGCCA GCTCCTCGAC
    CACTCCTTCG TCAAGGTCTG CACGGATGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91534
    Clone ID Related Accession (Same CDS sequence) XM_015472932.1 , XM_015464893.1
    Accession Version XM_015472932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGGGG TTCCAGCGTC CCTGAAAATG ACCGCCTGGC TTCCATCGCA
    GCTGAATTGC AGTTCAGGTC GCTGAGCCGC CACTCCAGCC CCACCGAGGA GCGCGAGGAA
    CCAGCATATC CAAAAGGTGA TTCAAGTGGG TCAACTCGAA GAAGTTGGGA ACTTCGGACG
    CTGATCAGTC AGACCAAGGA TACCGCTTCT AAGCAAGGAC CCATAGAAGC CATTCAGAAG
    TCAGTCCGGC TGTTTGAAGA AAAGCGGTAT CGAGAAATGA GGAGGAAAAA CATCATTGGT
    CAAGTCTGTG ACACCCCCAA GTCTTACGAC AACGTCATGC ACGTTGGCTT GAGGAAGGTG
    ACCTTTAAGT GGCAAAGAGG AAACAAAATC GGGGAAGGGC AGTACGGGAA GGTGTACACC
    TGCATCAACG TCGACACCGG CGAGCTGATG GCCATGAAGG AGATTCGATT TCAACCCAAC
    GACCATAAAA CCATCAAGGA AACTGCAGAC GAGCTGAAAA TCTTTGAAGG CATCAAGCAC
    CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTACATAGAG AGGAGATGTA CATCTTCATG
    GAGTACTGTG ACGAGGGCAC GTTGGAAGAG GTGTCGCGGC TGGGGCTTCA AGAACACGTC
    ATCAGGCTGT ATTCGAAACA GATTACCGTG GCCATCAACG TCCTGCACGA GCATGGCATA
    GTTCACCGCG ACGTTAAAGG TGCCAACATC TTCCTGACCT CGTCTGGACT AATCAAGCTG
    GGGGATTTCG GATGCTCAGT AAAGCTTAAG AACAATGCGC AGACCATGCC TGGTGAAGTG
    AACAGCACGC TGGGGACAGC AGCGTACATG GCTCCGGAAG TCATCACTCG CGCCAAAGGA
    GAAGGCCACG GGCGTGCGGC TGACATCTGG AGCCTGGGGT GTGTTGTCAT AGAGATGGTG
    ACTGGCAAGA GACCTTGGCA TGAATATGAA CACAACTTTC AGATTATGTA CAAAGTGGGA
    ATGGGGCACA AGCCGCCGAT CCCTGAACGA TTAAGCCCCG AAGGGAAGGA CTTCCTCTCT
    CACTGCCTGG AGAGCGAGCC CCGGATGCGG TGGACAGCCA GCCAGCTCCT CGACCACTCC
    TTCGTCAAGG TCTGCACGGA TGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328418.1
    CDS33..4622
    Translation

    Target ORF information:

    RefSeq Version XM_015472932.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGGGG TTCCAGCGTC CCTGAAAATG ACCGCCTGGC TTCCATCGCA
    GCTGAATTGC AGTTCAGGTC GCTGAGCCGC CACTCCAGCC CCACCGAGGA GCGCGAGGAA
    CCAGCATATC CAAAAGGTGA TTCAAGTGGG TCAACTCGAA GAAGTTGGGA ACTTCGGACG
    CTGATCAGTC AGACCAAGGA TACCGCTTCT AAGCAAGGAC CCATAGAAGC CATTCAGAAG
    TCAGTCCGGC TGTTTGAAGA AAAGCGGTAT CGAGAAATGA GGAGGAAAAA CATCATTGGT
    CAAGTCTGTG ACACCCCCAA GTCTTACGAC AACGTCATGC ACGTTGGCTT GAGGAAGGTG
    ACCTTTAAGT GGCAAAGAGG AAACAAAATC GGGGAAGGGC AGTACGGGAA GGTGTACACC
    TGCATCAACG TCGACACCGG CGAGCTGATG GCCATGAAGG AGATTCGATT TCAACCCAAC
    GACCATAAAA CCATCAAGGA AACTGCAGAC GAGCTGAAAA TCTTTGAAGG CATCAAGCAC
    CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTACATAGAG AGGAGATGTA CATCTTCATG
    GAGTACTGTG ACGAGGGCAC GTTGGAAGAG GTGTCGCGGC TGGGGCTTCA AGAACACGTC
    ATCAGGCTGT ATTCGAAACA GATTACCGTG GCCATCAACG TCCTGCACGA GCATGGCATA
    GTTCACCGCG ACGTTAAAGG TGCCAACATC TTCCTGACCT CGTCTGGACT AATCAAGCTG
    GGGGATTTCG GATGCTCAGT AAAGCTTAAG AACAATGCGC AGACCATGCC TGGTGAAGTG
    AACAGCACGC TGGGGACAGC AGCGTACATG GCTCCGGAAG TCATCACTCG CGCCAAAGGA
    GAAGGCCACG GGCGTGCGGC TGACATCTGG AGCCTGGGGT GTGTTGTCAT AGAGATGGTG
    ACTGGCAAGA GACCTTGGCA TGAATATGAA CACAACTTTC AGATTATGTA CAAAGTGGGA
    ATGGGGCACA AGCCGCCGAT CCCTGAACGA TTAAGCCCCG AAGGGAAGGA CTTCCTCTCT
    CACTGCCTGG AGAGCGAGCC CCGGATGCGG TGGACAGCCA GCCAGCTCCT CGACCACTCC
    TTCGTCAAGG TCTGCACGGA TGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91534
    Clone ID Related Accession (Same CDS sequence) XM_015472932.1 , XM_015464893.1
    Accession Version XM_015464893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGGGG TTCCAGCGTC CCTGAAAATG ACCGCCTGGC TTCCATCGCA
    GCTGAATTGC AGTTCAGGTC GCTGAGCCGC CACTCCAGCC CCACCGAGGA GCGCGAGGAA
    CCAGCATATC CAAAAGGTGA TTCAAGTGGG TCAACTCGAA GAAGTTGGGA ACTTCGGACG
    CTGATCAGTC AGACCAAGGA TACCGCTTCT AAGCAAGGAC CCATAGAAGC CATTCAGAAG
    TCAGTCCGGC TGTTTGAAGA AAAGCGGTAT CGAGAAATGA GGAGGAAAAA CATCATTGGT
    CAAGTCTGTG ACACCCCCAA GTCTTACGAC AACGTCATGC ACGTTGGCTT GAGGAAGGTG
    ACCTTTAAGT GGCAAAGAGG AAACAAAATC GGGGAAGGGC AGTACGGGAA GGTGTACACC
    TGCATCAACG TCGACACCGG CGAGCTGATG GCCATGAAGG AGATTCGATT TCAACCCAAC
    GACCATAAAA CCATCAAGGA AACTGCAGAC GAGCTGAAAA TCTTTGAAGG CATCAAGCAC
    CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTACATAGAG AGGAGATGTA CATCTTCATG
    GAGTACTGTG ACGAGGGCAC GTTGGAAGAG GTGTCGCGGC TGGGGCTTCA AGAACACGTC
    ATCAGGCTGT ATTCGAAACA GATTACCGTG GCCATCAACG TCCTGCACGA GCATGGCATA
    GTTCACCGCG ACGTTAAAGG TGCCAACATC TTCCTGACCT CGTCTGGACT AATCAAGCTG
    GGGGATTTCG GATGCTCAGT AAAGCTTAAG AACAATGCGC AGACCATGCC TGGTGAAGTG
    AACAGCACGC TGGGGACAGC AGCGTACATG GCTCCGGAAG TCATCACTCG CGCCAAAGGA
    GAAGGCCACG GGCGTGCGGC TGACATCTGG AGCCTGGGGT GTGTTGTCAT AGAGATGGTG
    ACTGGCAAGA GACCTTGGCA TGAATATGAA CACAACTTTC AGATTATGTA CAAAGTGGGA
    ATGGGGCACA AGCCGCCGAT CCCTGAACGA TTAAGCCCCG AAGGGAAGGA CTTCCTCTCT
    CACTGCCTGG AGAGCGAGCC CCGGATGCGG TGGACAGCCA GCCAGCTCCT CGACCACTCC
    TTCGTCAAGG TCTGCACGGA TGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320379.1
    CDS33..4622
    Translation

    Target ORF information:

    RefSeq Version XM_015464893.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGGGG TTCCAGCGTC CCTGAAAATG ACCGCCTGGC TTCCATCGCA
    GCTGAATTGC AGTTCAGGTC GCTGAGCCGC CACTCCAGCC CCACCGAGGA GCGCGAGGAA
    CCAGCATATC CAAAAGGTGA TTCAAGTGGG TCAACTCGAA GAAGTTGGGA ACTTCGGACG
    CTGATCAGTC AGACCAAGGA TACCGCTTCT AAGCAAGGAC CCATAGAAGC CATTCAGAAG
    TCAGTCCGGC TGTTTGAAGA AAAGCGGTAT CGAGAAATGA GGAGGAAAAA CATCATTGGT
    CAAGTCTGTG ACACCCCCAA GTCTTACGAC AACGTCATGC ACGTTGGCTT GAGGAAGGTG
    ACCTTTAAGT GGCAAAGAGG AAACAAAATC GGGGAAGGGC AGTACGGGAA GGTGTACACC
    TGCATCAACG TCGACACCGG CGAGCTGATG GCCATGAAGG AGATTCGATT TCAACCCAAC
    GACCATAAAA CCATCAAGGA AACTGCAGAC GAGCTGAAAA TCTTTGAAGG CATCAAGCAC
    CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTACATAGAG AGGAGATGTA CATCTTCATG
    GAGTACTGTG ACGAGGGCAC GTTGGAAGAG GTGTCGCGGC TGGGGCTTCA AGAACACGTC
    ATCAGGCTGT ATTCGAAACA GATTACCGTG GCCATCAACG TCCTGCACGA GCATGGCATA
    GTTCACCGCG ACGTTAAAGG TGCCAACATC TTCCTGACCT CGTCTGGACT AATCAAGCTG
    GGGGATTTCG GATGCTCAGT AAAGCTTAAG AACAATGCGC AGACCATGCC TGGTGAAGTG
    AACAGCACGC TGGGGACAGC AGCGTACATG GCTCCGGAAG TCATCACTCG CGCCAAAGGA
    GAAGGCCACG GGCGTGCGGC TGACATCTGG AGCCTGGGGT GTGTTGTCAT AGAGATGGTG
    ACTGGCAAGA GACCTTGGCA TGAATATGAA CACAACTTTC AGATTATGTA CAAAGTGGGA
    ATGGGGCACA AGCCGCCGAT CCCTGAACGA TTAAGCCCCG AAGGGAAGGA CTTCCTCTCT
    CACTGCCTGG AGAGCGAGCC CCGGATGCGG TGGACAGCCA GCCAGCTCCT CGACCACTCC
    TTCGTCAAGG TCTGCACGGA TGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91535
    Clone ID Related Accession (Same CDS sequence) XM_015464894.1 , XM_005211105.3
    Accession Version XM_015464894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGGGGTT CCAGCGTCCC TGAAAATGAC CGCCTGGCTT CCATCGCAGC TGAATTGCAG
    TTCAGGTCGC TGAGCCGCCA CTCCAGCCCC ACCGAGGAGC GCGAGGAACC AGCATATCCA
    AAAGGTGATT CAAGTGGGTC AACTCGAAGA AGTTGGGAAC TTCGGACGCT GATCAGTCAG
    ACCAAGGATA CCGCTTCTAA GCAAGGACCC ATAGAAGCCA TTCAGAAGTC AGTCCGGCTG
    TTTGAAGAAA AGCGGTATCG AGAAATGAGG AGGAAAAACA TCATTGGTCA AGTCTGTGAC
    ACCCCCAAGT CTTACGACAA CGTCATGCAC GTTGGCTTGA GGAAGGTGAC CTTTAAGTGG
    CAAAGAGGAA ACAAAATCGG GGAAGGGCAG TACGGGAAGG TGTACACCTG CATCAACGTC
    GACACCGGCG AGCTGATGGC CATGAAGGAG ATTCGATTTC AACCCAACGA CCATAAAACC
    ATCAAGGAAA CTGCAGACGA GCTGAAAATC TTTGAAGGCA TCAAGCACCC CAATCTGGTT
    CGGTATTTTG GTGTGGAGCT ACATAGAGAG GAGATGTACA TCTTCATGGA GTACTGTGAC
    GAGGGCACGT TGGAAGAGGT GTCGCGGCTG GGGCTTCAAG AACACGTCAT CAGGCTGTAT
    TCGAAACAGA TTACCGTGGC CATCAACGTC CTGCACGAGC ATGGCATAGT TCACCGCGAC
    GTTAAAGGTG CCAACATCTT CCTGACCTCG TCTGGACTAA TCAAGCTGGG GGATTTCGGA
    TGCTCAGTAA AGCTTAAGAA CAATGCGCAG ACCATGCCTG GTGAAGTGAA CAGCACGCTG
    GGGACAGCAG CGTACATGGC TCCGGAAGTC ATCACTCGCG CCAAAGGAGA AGGCCACGGG
    CGTGCGGCTG ACATCTGGAG CCTGGGGTGT GTTGTCATAG AGATGGTGAC TGGCAAGAGA
    CCTTGGCATG AATATGAACA CAACTTTCAG ATTATGTACA AAGTGGGAAT GGGGCACAAG
    CCGCCGATCC CTGAACGATT AAGCCCCGAA GGGAAGGACT TCCTCTCTCA CTGCCTGGAG
    AGCGAGCCCC GGATGCGGTG GACAGCCAGC CAGCTCCTCG ACCACTCCTT CGTCAAGGTC
    TGCACGGATG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320380.1
    CDS33..4610
    Translation

    Target ORF information:

    RefSeq Version XM_015464894.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGGGGTT CCAGCGTCCC TGAAAATGAC CGCCTGGCTT CCATCGCAGC TGAATTGCAG
    TTCAGGTCGC TGAGCCGCCA CTCCAGCCCC ACCGAGGAGC GCGAGGAACC AGCATATCCA
    AAAGGTGATT CAAGTGGGTC AACTCGAAGA AGTTGGGAAC TTCGGACGCT GATCAGTCAG
    ACCAAGGATA CCGCTTCTAA GCAAGGACCC ATAGAAGCCA TTCAGAAGTC AGTCCGGCTG
    TTTGAAGAAA AGCGGTATCG AGAAATGAGG AGGAAAAACA TCATTGGTCA AGTCTGTGAC
    ACCCCCAAGT CTTACGACAA CGTCATGCAC GTTGGCTTGA GGAAGGTGAC CTTTAAGTGG
    CAAAGAGGAA ACAAAATCGG GGAAGGGCAG TACGGGAAGG TGTACACCTG CATCAACGTC
    GACACCGGCG AGCTGATGGC CATGAAGGAG ATTCGATTTC AACCCAACGA CCATAAAACC
    ATCAAGGAAA CTGCAGACGA GCTGAAAATC TTTGAAGGCA TCAAGCACCC CAATCTGGTT
    CGGTATTTTG GTGTGGAGCT ACATAGAGAG GAGATGTACA TCTTCATGGA GTACTGTGAC
    GAGGGCACGT TGGAAGAGGT GTCGCGGCTG GGGCTTCAAG AACACGTCAT CAGGCTGTAT
    TCGAAACAGA TTACCGTGGC CATCAACGTC CTGCACGAGC ATGGCATAGT TCACCGCGAC
    GTTAAAGGTG CCAACATCTT CCTGACCTCG TCTGGACTAA TCAAGCTGGG GGATTTCGGA
    TGCTCAGTAA AGCTTAAGAA CAATGCGCAG ACCATGCCTG GTGAAGTGAA CAGCACGCTG
    GGGACAGCAG CGTACATGGC TCCGGAAGTC ATCACTCGCG CCAAAGGAGA AGGCCACGGG
    CGTGCGGCTG ACATCTGGAG CCTGGGGTGT GTTGTCATAG AGATGGTGAC TGGCAAGAGA
    CCTTGGCATG AATATGAACA CAACTTTCAG ATTATGTACA AAGTGGGAAT GGGGCACAAG
    CCGCCGATCC CTGAACGATT AAGCCCCGAA GGGAAGGACT TCCTCTCTCA CTGCCTGGAG
    AGCGAGCCCC GGATGCGGTG GACAGCCAGC CAGCTCCTCG ACCACTCCTT CGTCAAGGTC
    TGCACGGATG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91535
    Clone ID Related Accession (Same CDS sequence) XM_015464894.1 , XM_005211105.3
    Accession Version XM_005211105.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGGGGTT CCAGCGTCCC TGAAAATGAC CGCCTGGCTT CCATCGCAGC TGAATTGCAG
    TTCAGGTCGC TGAGCCGCCA CTCCAGCCCC ACCGAGGAGC GCGAGGAACC AGCATATCCA
    AAAGGTGATT CAAGTGGGTC AACTCGAAGA AGTTGGGAAC TTCGGACGCT GATCAGTCAG
    ACCAAGGATA CCGCTTCTAA GCAAGGACCC ATAGAAGCCA TTCAGAAGTC AGTCCGGCTG
    TTTGAAGAAA AGCGGTATCG AGAAATGAGG AGGAAAAACA TCATTGGTCA AGTCTGTGAC
    ACCCCCAAGT CTTACGACAA CGTCATGCAC GTTGGCTTGA GGAAGGTGAC CTTTAAGTGG
    CAAAGAGGAA ACAAAATCGG GGAAGGGCAG TACGGGAAGG TGTACACCTG CATCAACGTC
    GACACCGGCG AGCTGATGGC CATGAAGGAG ATTCGATTTC AACCCAACGA CCATAAAACC
    ATCAAGGAAA CTGCAGACGA GCTGAAAATC TTTGAAGGCA TCAAGCACCC CAATCTGGTT
    CGGTATTTTG GTGTGGAGCT ACATAGAGAG GAGATGTACA TCTTCATGGA GTACTGTGAC
    GAGGGCACGT TGGAAGAGGT GTCGCGGCTG GGGCTTCAAG AACACGTCAT CAGGCTGTAT
    TCGAAACAGA TTACCGTGGC CATCAACGTC CTGCACGAGC ATGGCATAGT TCACCGCGAC
    GTTAAAGGTG CCAACATCTT CCTGACCTCG TCTGGACTAA TCAAGCTGGG GGATTTCGGA
    TGCTCAGTAA AGCTTAAGAA CAATGCGCAG ACCATGCCTG GTGAAGTGAA CAGCACGCTG
    GGGACAGCAG CGTACATGGC TCCGGAAGTC ATCACTCGCG CCAAAGGAGA AGGCCACGGG
    CGTGCGGCTG ACATCTGGAG CCTGGGGTGT GTTGTCATAG AGATGGTGAC TGGCAAGAGA
    CCTTGGCATG AATATGAACA CAACTTTCAG ATTATGTACA AAGTGGGAAT GGGGCACAAG
    CCGCCGATCC CTGAACGATT AAGCCCCGAA GGGAAGGACT TCCTCTCTCA CTGCCTGGAG
    AGCGAGCCCC GGATGCGGTG GACAGCCAGC CAGCTCCTCG ACCACTCCTT CGTCAAGGTC
    TGCACGGATG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211162.2
    CDS33..4610
    Translation

    Target ORF information:

    RefSeq Version XM_005211105.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211105.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGGGGTT CCAGCGTCCC TGAAAATGAC CGCCTGGCTT CCATCGCAGC TGAATTGCAG
    TTCAGGTCGC TGAGCCGCCA CTCCAGCCCC ACCGAGGAGC GCGAGGAACC AGCATATCCA
    AAAGGTGATT CAAGTGGGTC AACTCGAAGA AGTTGGGAAC TTCGGACGCT GATCAGTCAG
    ACCAAGGATA CCGCTTCTAA GCAAGGACCC ATAGAAGCCA TTCAGAAGTC AGTCCGGCTG
    TTTGAAGAAA AGCGGTATCG AGAAATGAGG AGGAAAAACA TCATTGGTCA AGTCTGTGAC
    ACCCCCAAGT CTTACGACAA CGTCATGCAC GTTGGCTTGA GGAAGGTGAC CTTTAAGTGG
    CAAAGAGGAA ACAAAATCGG GGAAGGGCAG TACGGGAAGG TGTACACCTG CATCAACGTC
    GACACCGGCG AGCTGATGGC CATGAAGGAG ATTCGATTTC AACCCAACGA CCATAAAACC
    ATCAAGGAAA CTGCAGACGA GCTGAAAATC TTTGAAGGCA TCAAGCACCC CAATCTGGTT
    CGGTATTTTG GTGTGGAGCT ACATAGAGAG GAGATGTACA TCTTCATGGA GTACTGTGAC
    GAGGGCACGT TGGAAGAGGT GTCGCGGCTG GGGCTTCAAG AACACGTCAT CAGGCTGTAT
    TCGAAACAGA TTACCGTGGC CATCAACGTC CTGCACGAGC ATGGCATAGT TCACCGCGAC
    GTTAAAGGTG CCAACATCTT CCTGACCTCG TCTGGACTAA TCAAGCTGGG GGATTTCGGA
    TGCTCAGTAA AGCTTAAGAA CAATGCGCAG ACCATGCCTG GTGAAGTGAA CAGCACGCTG
    GGGACAGCAG CGTACATGGC TCCGGAAGTC ATCACTCGCG CCAAAGGAGA AGGCCACGGG
    CGTGCGGCTG ACATCTGGAG CCTGGGGTGT GTTGTCATAG AGATGGTGAC TGGCAAGAGA
    CCTTGGCATG AATATGAACA CAACTTTCAG ATTATGTACA AAGTGGGAAT GGGGCACAAG
    CCGCCGATCC CTGAACGATT AAGCCCCGAA GGGAAGGACT TCCTCTCTCA CTGCCTGGAG
    AGCGAGCCCC GGATGCGGTG GACAGCCAGC CAGCTCCTCG ACCACTCCTT CGTCAAGGTC
    TGCACGGATG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22804
    Clone ID Related Accession (Same CDS sequence) XM_002690377.6
    Accession Version XM_002690377.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690377.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGCCATTC ATCGGAATAG CCCCCGTCCT
    GTGAAGGTAC CTCGATGCCA TAGCGACCCT CCTAACCCTC ACCTGATTAT CCCAACTCCA
    GAGGGATTCA GCGCCCGGAG CGTGCCCGCG GATGTGCGGA GCCTCGGCAG CCCCGCGCCC
    GCGCCTGCCG CCGCTCGTCC TGGCCCCTCG GGCAGCGACC CAGCACCGCC CAAGCCCATC
    AGCGGTGCCC ATGAGACCAG GGGTTCCAGC GTCCCTGAAA ATGACCGCCT GGCTTCCATC
    GCAGCTGAAT TGCAGTTCAG GTCGCTGAGC CGCCACTCCA GCCCCACCGA GGAGCGCGAG
    GAACCAGCAT ATCCAAAAGG TGATTCAAGT GGGTCAACTC GAAGAAGTTG GGAACTTCGG
    ACGCTGATCA GTCAGACCAA GGATACCGCT TCTAAGCAAG GACCCATAGA AGCCATTCAG
    AAGTCAGTCC GGCTGTTTGA AGAAAAGCGG TATCGAGAAA TGAGGAGGAA AAACATCATT
    GGTCAAGTCT GTGACACCCC CAAGTCTTAC GACAACGTCA TGCACGTTGG CTTGAGGAAG
    GTGACCTTTA AGTGGCAAAG AGGAAACAAA ATCGGGGAAG GGCAGTACGG GAAGGTGTAC
    ACCTGCATCA ACGTCGACAC CGGCGAGCTG ATGGCCATGA AGGAGATTCG ATTTCAACCC
    AACGACCATA AAACCATCAA GGAAACTGCA GACGAGCTGA AAATCTTTGA AGGCATCAAG
    CACCCCAATC TGGTTCGGTA TTTTGGTGTG GAGCTACATA GAGAGGAGAT GTACATCTTC
    ATGGAGTACT GTGACGAGGG CACGTTGGAA GAGGTGTCGC GGCTGGGGCT TCAAGAACAC
    GTCATCAGGC TGTATTCGAA ACAGATTACC GTGGCCATCA ACGTCCTGCA CGAGCATGGC
    ATAGTTCACC GCGACGTTAA AGGTGCCAAC ATCTTCCTGA CCTCGTCTGG ACTAATCAAG
    CTGGGGGATT TCGGATGCTC AGTAAAGCTT AAGAACAATG CGCAGACCAT GCCTGGTGAA
    GTGAACAGCA CGCTGGGGAC AGCAGCGTAC ATGGCTCCGG AAGTCATCAC TCGCGCCAAA
    GGAGAAGGCC ACGGGCGTGC GGCTGACATC TGGAGCCTGG GGTGTGTTGT CATAGAGATG
    GTGACTGGCA AGAGACCTTG GCATGAATAT GAACACAACT TTCAGATTAT GTACAAAGTG
    GGAATGGGGC ACAAGCCGCC GATCCCTGAA CGATTAAGCC CCGAAGGGAA GGACTTCCTC
    TCTCACTGCC TGGAGAGCGA GCCCCGGATG CGGTGGACAG CCAGCCAGCT CCTCGACCAC
    TCCTTCGTCA AGGTCTGCAC GGATGAAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690423.4
    CDS24..4796
    Translation

    Target ORF information:

    RefSeq Version XM_002690377.6
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690377.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGCCATTC ATCGGAATAG CCCCCGTCCT
    GTGAAGGTAC CTCGATGCCA TAGCGACCCT CCTAACCCTC ACCTGATTAT CCCAACTCCA
    GAGGGATTCA GCGCCCGGAG CGTGCCCGCG GATGTGCGGA GCCTCGGCAG CCCCGCGCCC
    GCGCCTGCCG CCGCTCGTCC TGGCCCCTCG GGCAGCGACC CAGCACCGCC CAAGCCCATC
    AGCGGTGCCC ATGAGACCAG GGGTTCCAGC GTCCCTGAAA ATGACCGCCT GGCTTCCATC
    GCAGCTGAAT TGCAGTTCAG GTCGCTGAGC CGCCACTCCA GCCCCACCGA GGAGCGCGAG
    GAACCAGCAT ATCCAAAAGG TGATTCAAGT GGGTCAACTC GAAGAAGTTG GGAACTTCGG
    ACGCTGATCA GTCAGACCAA GGATACCGCT TCTAAGCAAG GACCCATAGA AGCCATTCAG
    AAGTCAGTCC GGCTGTTTGA AGAAAAGCGG TATCGAGAAA TGAGGAGGAA AAACATCATT
    GGTCAAGTCT GTGACACCCC CAAGTCTTAC GACAACGTCA TGCACGTTGG CTTGAGGAAG
    GTGACCTTTA AGTGGCAAAG AGGAAACAAA ATCGGGGAAG GGCAGTACGG GAAGGTGTAC
    ACCTGCATCA ACGTCGACAC CGGCGAGCTG ATGGCCATGA AGGAGATTCG ATTTCAACCC
    AACGACCATA AAACCATCAA GGAAACTGCA GACGAGCTGA AAATCTTTGA AGGCATCAAG
    CACCCCAATC TGGTTCGGTA TTTTGGTGTG GAGCTACATA GAGAGGAGAT GTACATCTTC
    ATGGAGTACT GTGACGAGGG CACGTTGGAA GAGGTGTCGC GGCTGGGGCT TCAAGAACAC
    GTCATCAGGC TGTATTCGAA ACAGATTACC GTGGCCATCA ACGTCCTGCA CGAGCATGGC
    ATAGTTCACC GCGACGTTAA AGGTGCCAAC ATCTTCCTGA CCTCGTCTGG ACTAATCAAG
    CTGGGGGATT TCGGATGCTC AGTAAAGCTT AAGAACAATG CGCAGACCAT GCCTGGTGAA
    GTGAACAGCA CGCTGGGGAC AGCAGCGTAC ATGGCTCCGG AAGTCATCAC TCGCGCCAAA
    GGAGAAGGCC ACGGGCGTGC GGCTGACATC TGGAGCCTGG GGTGTGTTGT CATAGAGATG
    GTGACTGGCA AGAGACCTTG GCATGAATAT GAACACAACT TTCAGATTAT GTACAAAGTG
    GGAATGGGGC ACAAGCCGCC GATCCCTGAA CGATTAAGCC CCGAAGGGAA GGACTTCCTC
    TCTCACTGCC TGGAGAGCGA GCCCCGGATG CGGTGGACAG CCAGCCAGCT CCTCGACCAC
    TCCTTCGTCA AGGTCTGCAC GGATGAAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22806
    Clone ID Related Accession (Same CDS sequence) XM_005211105.4
    Accession Version XM_005211105.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGCCATTC ATCGGAATAG CCCCCGTCCT
    GTGAAGGTAC CTCGATGCCA TAGCGACCCT CCTAACCCTC ACCTGATTAT CCCAACTCCA
    GAGGGATTCA GGGGTTCCAG CGTCCCTGAA AATGACCGCC TGGCTTCCAT CGCAGCTGAA
    TTGCAGTTCA GGTCGCTGAG CCGCCACTCC AGCCCCACCG AGGAGCGCGA GGAACCAGCA
    TATCCAAAAG GTGATTCAAG TGGGTCAACT CGAAGAAGTT GGGAACTTCG GACGCTGATC
    AGTCAGACCA AGGATACCGC TTCTAAGCAA GGACCCATAG AAGCCATTCA GAAGTCAGTC
    CGGCTGTTTG AAGAAAAGCG GTATCGAGAA ATGAGGAGGA AAAACATCAT TGGTCAAGTC
    TGTGACACCC CCAAGTCTTA CGACAACGTC ATGCACGTTG GCTTGAGGAA GGTGACCTTT
    AAGTGGCAAA GAGGAAACAA AATCGGGGAA GGGCAGTACG GGAAGGTGTA CACCTGCATC
    AACGTCGACA CCGGCGAGCT GATGGCCATG AAGGAGATTC GATTTCAACC CAACGACCAT
    AAAACCATCA AGGAAACTGC AGACGAGCTG AAAATCTTTG AAGGCATCAA GCACCCCAAT
    CTGGTTCGGT ATTTTGGTGT GGAGCTACAT AGAGAGGAGA TGTACATCTT CATGGAGTAC
    TGTGACGAGG GCACGTTGGA AGAGGTGTCG CGGCTGGGGC TTCAAGAACA CGTCATCAGG
    CTGTATTCGA AACAGATTAC CGTGGCCATC AACGTCCTGC ACGAGCATGG CATAGTTCAC
    CGCGACGTTA AAGGTGCCAA CATCTTCCTG ACCTCGTCTG GACTAATCAA GCTGGGGGAT
    TTCGGATGCT CAGTAAAGCT TAAGAACAAT GCGCAGACCA TGCCTGGTGA AGTGAACAGC
    ACGCTGGGGA CAGCAGCGTA CATGGCTCCG GAAGTCATCA CTCGCGCCAA AGGAGAAGGC
    CACGGGCGTG CGGCTGACAT CTGGAGCCTG GGGTGTGTTG TCATAGAGAT GGTGACTGGC
    AAGAGACCTT GGCATGAATA TGAACACAAC TTTCAGATTA TGTACAAAGT GGGAATGGGG
    CACAAGCCGC CGATCCCTGA ACGATTAAGC CCCGAAGGGA AGGACTTCCT CTCTCACTGC
    CTGGAGAGCG AGCCCCGGAT GCGGTGGACA GCCAGCCAGC TCCTCGACCA CTCCTTCGTC
    AAGGTCTGCA CGGATGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211162.3
    CDS51..4694
    Translation

    Target ORF information:

    RefSeq Version XM_005211105.4
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211105.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAGAAG CCGCCGCCGC CGCCGCGCCG GTGCCGCCGC TCGCCTGCGC CGTCACCTCA 
    GCCGCCATGG AGGAGCCGCC GCCGCCTCCG CCGCCAGAGC CTGAGCCCGA GCGCTGCCGG
    GCGGCGAGGC AAGAGTGCGT ATTGGGAGAC TCAGCTTGCA AGAATTCCGA ATCCGATCCT
    GAGGACTTTT CAGATGAAGC CAACACAGAG AACCTTTATG GCACCTCTCC TCCCAGCACG
    CCTCGGCAGA TGAAACGCAT GTCCTCCAAG CACCAGAGGA ATAGTGTCGG GAGGCCCGCC
    GGTCGCGCCA ATCTGAAAGA AAAAATGAAT GCATCAAACC AACCTCCACA CAGAGACACT
    GGAAAAACTG TGGAGAACGT GGAAGAATAC AGCTATAAGC AGGAGAAAAA GATCCGAGCG
    GCTCTCAGGT CCACGGAGCG GGATCATAAG AAGAATGTGC AGTGCTCGTT CATGCTGGAC
    TCAGTTGGGG GCTCTCTGCC CAAAAAGTCC ATCCCGGATG TGGATCTCAA TAAGCCTTAC
    CTCAGTCTCG GCTGCAGCAA TGCTAAGCTT CCGGTCTCTG TGCCCATGCC GATAGCCAGA
    ACCGCCCGCC AGACTTCCAG GACTGACTGT CCGGCAGATC GCTTAAAATT TTTTGAAACT
    CTCCGACTTC TGCTAAAGCT TACCTCAGTC TCAAAGAAGA AGGACAGGGA GCAAAGAGGC
    CAGGAAAGCA CATCCGCGTT CTGGTTTAAC CGATCTAACG AGCTGATCTG GTTAGAGCTG
    CAAGCCTGGC ACGCCGGCCG GACCATTAAC GACCAGGACC TCTTTTTATA CACAGCCCGC
    CAGGCCATCC CAGACATTAT CAACGAAATC CTTACCTTCA AAGTTAACTA TGGGAGCTTC
    GCCTTCGTTA GAAACGGAGC TAGTCTTAAC GGTACTTCAG TAGAAGGGCA GTTCGGAGCC
    CCTCACGGGA CGCAGCCTGC GGGTTACTCA ACCTATCACG AGCACCTCCA ACGCCAGAGG
    GTGTCCTTCG AGCAGGTCAA ACGGATAATG GAGCTGCTGG AGTACATAGA GGCACTTTAT
    CCATCGATGC AGGCTCTTCA AAAGGATTAT GAGAAGTATG CTGCAAAGGA CTTCCAGGAC
    AGGGTGCAGG CGCTCTGTTT GTGGTTAAAC ATCACAAAGG ACTTAAATCA GAAATTAAGG
    ATTATGGGCA CCGTGTTGGG CATCAAGAAT TTATCAGACA TTGGCTGGCC GGTGTTTGAA
    ATCCCCTCCC CGCGATCATC CAGGGGCAAC GAGCCCGAGG ATGAGGCGGA GGACACAGAA
    GGAGAGCTGA AGGAGCTGGA CAGCAGCACG GAGGAGAGTG AGGAAGAGCA GAGCTGCGGC
    CCGAGGGCAC CGGAGCCCAG GCCGCCCGCC GACCACAGCC GCCACGTCCA GTCGCCCCCG
    GACTGTGTCC CGAAGAAGCT CGAGAGGCTG GAGTCGGAGG ACGAGTCTGT CGGCTGGGGA
    GCCCCAGACT GGAGCGCCGA GGCAGGCGTT AGTGGACACT GTCTGACCTC TATCTACAGA
    CCCTTTGTAG ACAAAGCGCT GAAGCAGATG GGCTTAAGGA AGTTGATTCT AAGGCTTCAC
    AAGCTGATGG ACGGTTCCTT GCAGCGGGCA CGTATAGCAC TAGTTAAGAG CGACGGTCCA
    GTGGAGTTTT CTGGATTTCC AGATCCCATG TGGGGTTCAG ATTATGTGCA GTTGTCCAGG
    ACGCCGCCTT CCTCGGAGCA GAAGTGCAGC ACCGTGTCCT GGGAGGAGCT GAGGTCCATG
    GATTTACCCT CCTTCGAGCC TGCCTTCCTG GTCCTCTGTC GAGTCCTTCT GAACGTCATT
    CACGAATGTC TCAAGTTAAG GCTGGAACAG AGGCCCGCTG GAGAGCCATC CCTCTTGAGT
    ATTAAGCAGC TGGTGAGAGA ATGTAAGGAG GTGCTGAAGG GCGGCCTCCT GATGAAGCAG
    TACTACCAGT TCATGCTGCA CGAGGTCCTA GACCAGCTGG AGAAGACCGA CTGCAACATC
    GACGCCTTCG AGGAGGACCT GCACAAAATG CTGATGGTAT ATTTTGATTA CATGAGAAGC
    TGGATCCAAA TGCTACAGGA ATTACCTCAA GCATCTCATA GTTTAAAAAA TTTGTTAGAA
    GAAGAATGGA ATTTCACCAA AGAAATAACC CATTACATCC GGGGAGGAGA AGCACAGGCT
    GGAAAGCTTT TCTGTGACAT TGCAGGAATG CTGCTGAAAT CAACAGGAAG TTTTCTAGAG
    TCTGGCTTAC AGGAGAGCTG TGCGGAATTC TGGACCAGTG CCGACGACAG CAGCGCTTCA
    GATGAAATAA GGAGGTCGGT TATAGAGGTC AGCCGAGCAC TGAAGGAGCT CTTCCACGAG
    GCCAGGGAAC GAGCCTCCAA GGCACTGGGG TTTGCCAAGA TGCTGAGAAA GGACCTGGAA
    ATAGCTGCGG AGTTCATACT CTCGGCCCCG GTTAGAGACC TTCTGGACGT TCTGAAAGCA
    AAGCAGTACG TTAAGGTACA GATTCCTGGC TTAGAAAACT TGCAGGTGTT TGTTCCAGAC
    GCTCTTGCTG AGGAGAAGAG CATTATTTTG CAGTTACTCA ATGCAGCTAC AGGGAAGGAC
    TGCTCCAAAG ATTCAGATGA AGCACTAATT GACGACTATC TACTTTTGAC CAAGCATGGT
    GACCGCACTC CAGACTCGGA GGCCAGCTGG GCCGCCTGGG AGGCGCAGCC TGTCAAGGTC
    GTGCCTCCGG TGGAGACAGT GGACACCCTG AGAAGCATGC AGGTGGACAA TCTTCTACTA
    GTTGTCATGC ACTCTGCGCA GCTCATAATC CATAGAAAAG CTTTCCAGCA GTCCATCGAA
    GGACTCATGA CCCTACGCCA GGAGCAGACT TCCAGTCAGC CCGTCATCGC CAAAGCTTTG
    CAGCAGCTGA AGAATGATGC CTTAGAGCTC TGCAACCGAA TAAGTGATGC CATTGACCGA
    GTGGACCACA TGTTCACTTC AGAATTTGAT GCTGAAGTTG ATGAATCTGA GTCTGCTACA
    CTGCAGCAGT ATTACCGAGA AGCCATGATC CAAGGGTACA ATTTCGGGTT CGAGTATCAT
    AAAGAAGTTG TTCGTTTGAT GTCTGGCGAG TTTAAACAGA AGATAGGAGA CAAGTATATA
    AGCTTTGCCC GGAAATGGAT GAATTACGTC CTGACTAAGT GTGAGAGCGG CAGAGGGACG
    AGACCCAGGT GGGCAACTCA AGGTTTTGAC TTCCTGCAAG CCATCGAGCC TTCGTTCATC
    TCTGCTCTGC CAGAAGACGA CTTCCTGAGT TTGCAAGCCC TGATGAATGA GTGCATTGGC
    CATGTGATAG GAAAGCCGCA CAGCCCTATT ACAGCCATTC ATCGGAATAG CCCCCGTCCT
    GTGAAGGTAC CTCGATGCCA TAGCGACCCT CCTAACCCTC ACCTGATTAT CCCAACTCCA
    GAGGGATTCA GGGGTTCCAG CGTCCCTGAA AATGACCGCC TGGCTTCCAT CGCAGCTGAA
    TTGCAGTTCA GGTCGCTGAG CCGCCACTCC AGCCCCACCG AGGAGCGCGA GGAACCAGCA
    TATCCAAAAG GTGATTCAAG TGGGTCAACT CGAAGAAGTT GGGAACTTCG GACGCTGATC
    AGTCAGACCA AGGATACCGC TTCTAAGCAA GGACCCATAG AAGCCATTCA GAAGTCAGTC
    CGGCTGTTTG AAGAAAAGCG GTATCGAGAA ATGAGGAGGA AAAACATCAT TGGTCAAGTC
    TGTGACACCC CCAAGTCTTA CGACAACGTC ATGCACGTTG GCTTGAGGAA GGTGACCTTT
    AAGTGGCAAA GAGGAAACAA AATCGGGGAA GGGCAGTACG GGAAGGTGTA CACCTGCATC
    AACGTCGACA CCGGCGAGCT GATGGCCATG AAGGAGATTC GATTTCAACC CAACGACCAT
    AAAACCATCA AGGAAACTGC AGACGAGCTG AAAATCTTTG AAGGCATCAA GCACCCCAAT
    CTGGTTCGGT ATTTTGGTGT GGAGCTACAT AGAGAGGAGA TGTACATCTT CATGGAGTAC
    TGTGACGAGG GCACGTTGGA AGAGGTGTCG CGGCTGGGGC TTCAAGAACA CGTCATCAGG
    CTGTATTCGA AACAGATTAC CGTGGCCATC AACGTCCTGC ACGAGCATGG CATAGTTCAC
    CGCGACGTTA AAGGTGCCAA CATCTTCCTG ACCTCGTCTG GACTAATCAA GCTGGGGGAT
    TTCGGATGCT CAGTAAAGCT TAAGAACAAT GCGCAGACCA TGCCTGGTGA AGTGAACAGC
    ACGCTGGGGA CAGCAGCGTA CATGGCTCCG GAAGTCATCA CTCGCGCCAA AGGAGAAGGC
    CACGGGCGTG CGGCTGACAT CTGGAGCCTG GGGTGTGTTG TCATAGAGAT GGTGACTGGC
    AAGAGACCTT GGCATGAATA TGAACACAAC TTTCAGATTA TGTACAAAGT GGGAATGGGG
    CACAAGCCGC CGATCCCTGA ACGATTAAGC CCCGAAGGGA AGGACTTCCT CTCTCACTGC
    CTGGAGAGCG AGCCCCGGAT GCGGTGGACA GCCAGCCAGC TCCTCGACCA CTCCTTCGTC
    AAGGTCTGCA CGGATGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91532
    Clone ID Related Accession (Same CDS sequence) XM_015464891.1 , XM_015472931.1
    Accession Version XM_015464891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGCGC CCGGAGCGTG CCCGCGGATG TGCGGAGCCT CGGCAGCCCC
    GCGCCCGCGC CTGCCGCCGC TCGTCCTGGC CCCTCGGGCA GCGACCCAGC ACCGCCCAAG
    CCCATCAGCG GTGCCCATGA GACCAGGGGT TCCAGCGTCC CTGAAAATGA CCGCCTGGCT
    TCCATCGCAG CTGAATTGCA GTTCAGGTCG CTGAGCCGCC ACTCCAGCCC CACCGAGGAG
    CGCGAGGAAC CAGCATATCC AAAAGGTGAT TCAAGTGGGT CAACTCGAAG AAGTTGGGAA
    CTTCGGACGC TGATCAGTCA GACCAAGGAT ACCGCTTCTA AGCAAGGACC CATAGAAGCC
    ATTCAGAAGT CAGTCCGGCT GTTTGAAGAA AAGCGGTATC GAGAAATGAG GAGGAAAAAC
    ATCATTGGTC AAGTCTGTGA CACCCCCAAG TCTTACGACA ACGTCATGCA CGTTGGCTTG
    AGGAAGGTGA CCTTTAAGTG GCAAAGAGGA AACAAAATCG GGGAAGGGCA GTACGGGAAG
    GTGTACACCT GCATCAACGT CGACACCGGC GAGCTGATGG CCATGAAGGA GATTCGATTT
    CAACCCAACG ACCATAAAAC CATCAAGGAA ACTGCAGACG AGCTGAAAAT CTTTGAAGGC
    ATCAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TACATAGAGA GGAGATGTAC
    ATCTTCATGG AGTACTGTGA CGAGGGCACG TTGGAAGAGG TGTCGCGGCT GGGGCTTCAA
    GAACACGTCA TCAGGCTGTA TTCGAAACAG ATTACCGTGG CCATCAACGT CCTGCACGAG
    CATGGCATAG TTCACCGCGA CGTTAAAGGT GCCAACATCT TCCTGACCTC GTCTGGACTA
    ATCAAGCTGG GGGATTTCGG ATGCTCAGTA AAGCTTAAGA ACAATGCGCA GACCATGCCT
    GGTGAAGTGA ACAGCACGCT GGGGACAGCA GCGTACATGG CTCCGGAAGT CATCACTCGC
    GCCAAAGGAG AAGGCCACGG GCGTGCGGCT GACATCTGGA GCCTGGGGTG TGTTGTCATA
    GAGATGGTGA CTGGCAAGAG ACCTTGGCAT GAATATGAAC ACAACTTTCA GATTATGTAC
    AAAGTGGGAA TGGGGCACAA GCCGCCGATC CCTGAACGAT TAAGCCCCGA AGGGAAGGAC
    TTCCTCTCTC ACTGCCTGGA GAGCGAGCCC CGGATGCGGT GGACAGCCAG CCAGCTCCTC
    GACCACTCCT TCGTCAAGGT CTGCACGGAT GAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320377.1
    CDS33..4751
    Translation

    Target ORF information:

    RefSeq Version XM_015464891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGCGC CCGGAGCGTG CCCGCGGATG TGCGGAGCCT CGGCAGCCCC
    GCGCCCGCGC CTGCCGCCGC TCGTCCTGGC CCCTCGGGCA GCGACCCAGC ACCGCCCAAG
    CCCATCAGCG GTGCCCATGA GACCAGGGGT TCCAGCGTCC CTGAAAATGA CCGCCTGGCT
    TCCATCGCAG CTGAATTGCA GTTCAGGTCG CTGAGCCGCC ACTCCAGCCC CACCGAGGAG
    CGCGAGGAAC CAGCATATCC AAAAGGTGAT TCAAGTGGGT CAACTCGAAG AAGTTGGGAA
    CTTCGGACGC TGATCAGTCA GACCAAGGAT ACCGCTTCTA AGCAAGGACC CATAGAAGCC
    ATTCAGAAGT CAGTCCGGCT GTTTGAAGAA AAGCGGTATC GAGAAATGAG GAGGAAAAAC
    ATCATTGGTC AAGTCTGTGA CACCCCCAAG TCTTACGACA ACGTCATGCA CGTTGGCTTG
    AGGAAGGTGA CCTTTAAGTG GCAAAGAGGA AACAAAATCG GGGAAGGGCA GTACGGGAAG
    GTGTACACCT GCATCAACGT CGACACCGGC GAGCTGATGG CCATGAAGGA GATTCGATTT
    CAACCCAACG ACCATAAAAC CATCAAGGAA ACTGCAGACG AGCTGAAAAT CTTTGAAGGC
    ATCAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TACATAGAGA GGAGATGTAC
    ATCTTCATGG AGTACTGTGA CGAGGGCACG TTGGAAGAGG TGTCGCGGCT GGGGCTTCAA
    GAACACGTCA TCAGGCTGTA TTCGAAACAG ATTACCGTGG CCATCAACGT CCTGCACGAG
    CATGGCATAG TTCACCGCGA CGTTAAAGGT GCCAACATCT TCCTGACCTC GTCTGGACTA
    ATCAAGCTGG GGGATTTCGG ATGCTCAGTA AAGCTTAAGA ACAATGCGCA GACCATGCCT
    GGTGAAGTGA ACAGCACGCT GGGGACAGCA GCGTACATGG CTCCGGAAGT CATCACTCGC
    GCCAAAGGAG AAGGCCACGG GCGTGCGGCT GACATCTGGA GCCTGGGGTG TGTTGTCATA
    GAGATGGTGA CTGGCAAGAG ACCTTGGCAT GAATATGAAC ACAACTTTCA GATTATGTAC
    AAAGTGGGAA TGGGGCACAA GCCGCCGATC CCTGAACGAT TAAGCCCCGA AGGGAAGGAC
    TTCCTCTCTC ACTGCCTGGA GAGCGAGCCC CGGATGCGGT GGACAGCCAG CCAGCTCCTC
    GACCACTCCT TCGTCAAGGT CTGCACGGAT GAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91532
    Clone ID Related Accession (Same CDS sequence) XM_015464891.1 , XM_015472931.1
    Accession Version XM_015472931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGCGC CCGGAGCGTG CCCGCGGATG TGCGGAGCCT CGGCAGCCCC
    GCGCCCGCGC CTGCCGCCGC TCGTCCTGGC CCCTCGGGCA GCGACCCAGC ACCGCCCAAG
    CCCATCAGCG GTGCCCATGA GACCAGGGGT TCCAGCGTCC CTGAAAATGA CCGCCTGGCT
    TCCATCGCAG CTGAATTGCA GTTCAGGTCG CTGAGCCGCC ACTCCAGCCC CACCGAGGAG
    CGCGAGGAAC CAGCATATCC AAAAGGTGAT TCAAGTGGGT CAACTCGAAG AAGTTGGGAA
    CTTCGGACGC TGATCAGTCA GACCAAGGAT ACCGCTTCTA AGCAAGGACC CATAGAAGCC
    ATTCAGAAGT CAGTCCGGCT GTTTGAAGAA AAGCGGTATC GAGAAATGAG GAGGAAAAAC
    ATCATTGGTC AAGTCTGTGA CACCCCCAAG TCTTACGACA ACGTCATGCA CGTTGGCTTG
    AGGAAGGTGA CCTTTAAGTG GCAAAGAGGA AACAAAATCG GGGAAGGGCA GTACGGGAAG
    GTGTACACCT GCATCAACGT CGACACCGGC GAGCTGATGG CCATGAAGGA GATTCGATTT
    CAACCCAACG ACCATAAAAC CATCAAGGAA ACTGCAGACG AGCTGAAAAT CTTTGAAGGC
    ATCAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TACATAGAGA GGAGATGTAC
    ATCTTCATGG AGTACTGTGA CGAGGGCACG TTGGAAGAGG TGTCGCGGCT GGGGCTTCAA
    GAACACGTCA TCAGGCTGTA TTCGAAACAG ATTACCGTGG CCATCAACGT CCTGCACGAG
    CATGGCATAG TTCACCGCGA CGTTAAAGGT GCCAACATCT TCCTGACCTC GTCTGGACTA
    ATCAAGCTGG GGGATTTCGG ATGCTCAGTA AAGCTTAAGA ACAATGCGCA GACCATGCCT
    GGTGAAGTGA ACAGCACGCT GGGGACAGCA GCGTACATGG CTCCGGAAGT CATCACTCGC
    GCCAAAGGAG AAGGCCACGG GCGTGCGGCT GACATCTGGA GCCTGGGGTG TGTTGTCATA
    GAGATGGTGA CTGGCAAGAG ACCTTGGCAT GAATATGAAC ACAACTTTCA GATTATGTAC
    AAAGTGGGAA TGGGGCACAA GCCGCCGATC CCTGAACGAT TAAGCCCCGA AGGGAAGGAC
    TTCCTCTCTC ACTGCCTGGA GAGCGAGCCC CGGATGCGGT GGACAGCCAG CCAGCTCCTC
    GACCACTCCT TCGTCAAGGT CTGCACGGAT GAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328417.1
    CDS33..4751
    Translation

    Target ORF information:

    RefSeq Version XM_015472931.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGGT TTGTACTTTG CCATTCATCG GAATAGCCCC
    CGTCCTGTGA AGGTACCTCG ATGCCATAGC GACCCTCCTA ACCCTCACCT GATTATCCCA
    ACTCCAGAGG GATTCAGCGC CCGGAGCGTG CCCGCGGATG TGCGGAGCCT CGGCAGCCCC
    GCGCCCGCGC CTGCCGCCGC TCGTCCTGGC CCCTCGGGCA GCGACCCAGC ACCGCCCAAG
    CCCATCAGCG GTGCCCATGA GACCAGGGGT TCCAGCGTCC CTGAAAATGA CCGCCTGGCT
    TCCATCGCAG CTGAATTGCA GTTCAGGTCG CTGAGCCGCC ACTCCAGCCC CACCGAGGAG
    CGCGAGGAAC CAGCATATCC AAAAGGTGAT TCAAGTGGGT CAACTCGAAG AAGTTGGGAA
    CTTCGGACGC TGATCAGTCA GACCAAGGAT ACCGCTTCTA AGCAAGGACC CATAGAAGCC
    ATTCAGAAGT CAGTCCGGCT GTTTGAAGAA AAGCGGTATC GAGAAATGAG GAGGAAAAAC
    ATCATTGGTC AAGTCTGTGA CACCCCCAAG TCTTACGACA ACGTCATGCA CGTTGGCTTG
    AGGAAGGTGA CCTTTAAGTG GCAAAGAGGA AACAAAATCG GGGAAGGGCA GTACGGGAAG
    GTGTACACCT GCATCAACGT CGACACCGGC GAGCTGATGG CCATGAAGGA GATTCGATTT
    CAACCCAACG ACCATAAAAC CATCAAGGAA ACTGCAGACG AGCTGAAAAT CTTTGAAGGC
    ATCAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TACATAGAGA GGAGATGTAC
    ATCTTCATGG AGTACTGTGA CGAGGGCACG TTGGAAGAGG TGTCGCGGCT GGGGCTTCAA
    GAACACGTCA TCAGGCTGTA TTCGAAACAG ATTACCGTGG CCATCAACGT CCTGCACGAG
    CATGGCATAG TTCACCGCGA CGTTAAAGGT GCCAACATCT TCCTGACCTC GTCTGGACTA
    ATCAAGCTGG GGGATTTCGG ATGCTCAGTA AAGCTTAAGA ACAATGCGCA GACCATGCCT
    GGTGAAGTGA ACAGCACGCT GGGGACAGCA GCGTACATGG CTCCGGAAGT CATCACTCGC
    GCCAAAGGAG AAGGCCACGG GCGTGCGGCT GACATCTGGA GCCTGGGGTG TGTTGTCATA
    GAGATGGTGA CTGGCAAGAG ACCTTGGCAT GAATATGAAC ACAACTTTCA GATTATGTAC
    AAAGTGGGAA TGGGGCACAA GCCGCCGATC CCTGAACGAT TAAGCCCCGA AGGGAAGGAC
    TTCCTCTCTC ACTGCCTGGA GAGCGAGCCC CGGATGCGGT GGACAGCCAG CCAGCTCCTC
    GACCACTCCT TCGTCAAGGT CTGCACGGAT GAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91533
    Clone ID Related Accession (Same CDS sequence) XM_002690377.5 , XM_015464892.1
    Accession Version XM_002690377.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690377.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGCGCCC GGAGCGTGCC CGCGGATGTG CGGAGCCTCG GCAGCCCCGC GCCCGCGCCT
    GCCGCCGCTC GTCCTGGCCC CTCGGGCAGC GACCCAGCAC CGCCCAAGCC CATCAGCGGT
    GCCCATGAGA CCAGGGGTTC CAGCGTCCCT GAAAATGACC GCCTGGCTTC CATCGCAGCT
    GAATTGCAGT TCAGGTCGCT GAGCCGCCAC TCCAGCCCCA CCGAGGAGCG CGAGGAACCA
    GCATATCCAA AAGGTGATTC AAGTGGGTCA ACTCGAAGAA GTTGGGAACT TCGGACGCTG
    ATCAGTCAGA CCAAGGATAC CGCTTCTAAG CAAGGACCCA TAGAAGCCAT TCAGAAGTCA
    GTCCGGCTGT TTGAAGAAAA GCGGTATCGA GAAATGAGGA GGAAAAACAT CATTGGTCAA
    GTCTGTGACA CCCCCAAGTC TTACGACAAC GTCATGCACG TTGGCTTGAG GAAGGTGACC
    TTTAAGTGGC AAAGAGGAAA CAAAATCGGG GAAGGGCAGT ACGGGAAGGT GTACACCTGC
    ATCAACGTCG ACACCGGCGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA ACCCAACGAC
    CATAAAACCA TCAAGGAAAC TGCAGACGAG CTGAAAATCT TTGAAGGCAT CAAGCACCCC
    AATCTGGTTC GGTATTTTGG TGTGGAGCTA CATAGAGAGG AGATGTACAT CTTCATGGAG
    TACTGTGACG AGGGCACGTT GGAAGAGGTG TCGCGGCTGG GGCTTCAAGA ACACGTCATC
    AGGCTGTATT CGAAACAGAT TACCGTGGCC ATCAACGTCC TGCACGAGCA TGGCATAGTT
    CACCGCGACG TTAAAGGTGC CAACATCTTC CTGACCTCGT CTGGACTAAT CAAGCTGGGG
    GATTTCGGAT GCTCAGTAAA GCTTAAGAAC AATGCGCAGA CCATGCCTGG TGAAGTGAAC
    AGCACGCTGG GGACAGCAGC GTACATGGCT CCGGAAGTCA TCACTCGCGC CAAAGGAGAA
    GGCCACGGGC GTGCGGCTGA CATCTGGAGC CTGGGGTGTG TTGTCATAGA GATGGTGACT
    GGCAAGAGAC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTACAA AGTGGGAATG
    GGGCACAAGC CGCCGATCCC TGAACGATTA AGCCCCGAAG GGAAGGACTT CCTCTCTCAC
    TGCCTGGAGA GCGAGCCCCG GATGCGGTGG ACAGCCAGCC AGCTCCTCGA CCACTCCTTC
    GTCAAGGTCT GCACGGATGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690423.3
    CDS33..4739
    Translation

    Target ORF information:

    RefSeq Version XM_002690377.5
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690377.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGCGCCC GGAGCGTGCC CGCGGATGTG CGGAGCCTCG GCAGCCCCGC GCCCGCGCCT
    GCCGCCGCTC GTCCTGGCCC CTCGGGCAGC GACCCAGCAC CGCCCAAGCC CATCAGCGGT
    GCCCATGAGA CCAGGGGTTC CAGCGTCCCT GAAAATGACC GCCTGGCTTC CATCGCAGCT
    GAATTGCAGT TCAGGTCGCT GAGCCGCCAC TCCAGCCCCA CCGAGGAGCG CGAGGAACCA
    GCATATCCAA AAGGTGATTC AAGTGGGTCA ACTCGAAGAA GTTGGGAACT TCGGACGCTG
    ATCAGTCAGA CCAAGGATAC CGCTTCTAAG CAAGGACCCA TAGAAGCCAT TCAGAAGTCA
    GTCCGGCTGT TTGAAGAAAA GCGGTATCGA GAAATGAGGA GGAAAAACAT CATTGGTCAA
    GTCTGTGACA CCCCCAAGTC TTACGACAAC GTCATGCACG TTGGCTTGAG GAAGGTGACC
    TTTAAGTGGC AAAGAGGAAA CAAAATCGGG GAAGGGCAGT ACGGGAAGGT GTACACCTGC
    ATCAACGTCG ACACCGGCGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA ACCCAACGAC
    CATAAAACCA TCAAGGAAAC TGCAGACGAG CTGAAAATCT TTGAAGGCAT CAAGCACCCC
    AATCTGGTTC GGTATTTTGG TGTGGAGCTA CATAGAGAGG AGATGTACAT CTTCATGGAG
    TACTGTGACG AGGGCACGTT GGAAGAGGTG TCGCGGCTGG GGCTTCAAGA ACACGTCATC
    AGGCTGTATT CGAAACAGAT TACCGTGGCC ATCAACGTCC TGCACGAGCA TGGCATAGTT
    CACCGCGACG TTAAAGGTGC CAACATCTTC CTGACCTCGT CTGGACTAAT CAAGCTGGGG
    GATTTCGGAT GCTCAGTAAA GCTTAAGAAC AATGCGCAGA CCATGCCTGG TGAAGTGAAC
    AGCACGCTGG GGACAGCAGC GTACATGGCT CCGGAAGTCA TCACTCGCGC CAAAGGAGAA
    GGCCACGGGC GTGCGGCTGA CATCTGGAGC CTGGGGTGTG TTGTCATAGA GATGGTGACT
    GGCAAGAGAC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTACAA AGTGGGAATG
    GGGCACAAGC CGCCGATCCC TGAACGATTA AGCCCCGAAG GGAAGGACTT CCTCTCTCAC
    TGCCTGGAGA GCGAGCCCCG GATGCGGTGG ACAGCCAGCC AGCTCCTCGA CCACTCCTTC
    GTCAAGGTCT GCACGGATGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91533
    Clone ID Related Accession (Same CDS sequence) XM_002690377.5 , XM_015464892.1
    Accession Version XM_015464892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGCGCCC GGAGCGTGCC CGCGGATGTG CGGAGCCTCG GCAGCCCCGC GCCCGCGCCT
    GCCGCCGCTC GTCCTGGCCC CTCGGGCAGC GACCCAGCAC CGCCCAAGCC CATCAGCGGT
    GCCCATGAGA CCAGGGGTTC CAGCGTCCCT GAAAATGACC GCCTGGCTTC CATCGCAGCT
    GAATTGCAGT TCAGGTCGCT GAGCCGCCAC TCCAGCCCCA CCGAGGAGCG CGAGGAACCA
    GCATATCCAA AAGGTGATTC AAGTGGGTCA ACTCGAAGAA GTTGGGAACT TCGGACGCTG
    ATCAGTCAGA CCAAGGATAC CGCTTCTAAG CAAGGACCCA TAGAAGCCAT TCAGAAGTCA
    GTCCGGCTGT TTGAAGAAAA GCGGTATCGA GAAATGAGGA GGAAAAACAT CATTGGTCAA
    GTCTGTGACA CCCCCAAGTC TTACGACAAC GTCATGCACG TTGGCTTGAG GAAGGTGACC
    TTTAAGTGGC AAAGAGGAAA CAAAATCGGG GAAGGGCAGT ACGGGAAGGT GTACACCTGC
    ATCAACGTCG ACACCGGCGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA ACCCAACGAC
    CATAAAACCA TCAAGGAAAC TGCAGACGAG CTGAAAATCT TTGAAGGCAT CAAGCACCCC
    AATCTGGTTC GGTATTTTGG TGTGGAGCTA CATAGAGAGG AGATGTACAT CTTCATGGAG
    TACTGTGACG AGGGCACGTT GGAAGAGGTG TCGCGGCTGG GGCTTCAAGA ACACGTCATC
    AGGCTGTATT CGAAACAGAT TACCGTGGCC ATCAACGTCC TGCACGAGCA TGGCATAGTT
    CACCGCGACG TTAAAGGTGC CAACATCTTC CTGACCTCGT CTGGACTAAT CAAGCTGGGG
    GATTTCGGAT GCTCAGTAAA GCTTAAGAAC AATGCGCAGA CCATGCCTGG TGAAGTGAAC
    AGCACGCTGG GGACAGCAGC GTACATGGCT CCGGAAGTCA TCACTCGCGC CAAAGGAGAA
    GGCCACGGGC GTGCGGCTGA CATCTGGAGC CTGGGGTGTG TTGTCATAGA GATGGTGACT
    GGCAAGAGAC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTACAA AGTGGGAATG
    GGGCACAAGC CGCCGATCCC TGAACGATTA AGCCCCGAAG GGAAGGACTT CCTCTCTCAC
    TGCCTGGAGA GCGAGCCCCG GATGCGGTGG ACAGCCAGCC AGCTCCTCGA CCACTCCTTC
    GTCAAGGTCT GCACGGATGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320378.1
    CDS33..4739
    Translation

    Target ORF information:

    RefSeq Version XM_015464892.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC CGCCGCCGCC TCCGCCGCCA GAGCCTGAGC CCGAGCGCTG CCGGGCGGCG 
    AGGCAAGAGT GCGTATTGGG AGACTCAGCT TGCAAGAATT CCGAATCCGA TCCTGAGGAC
    TTTTCAGATG AAGCCAACAC AGAGAACCTT TATGGCACCT CTCCTCCCAG CACGCCTCGG
    CAGATGAAAC GCATGTCCTC CAAGCACCAG AGGAATAGTG TCGGGAGGCC CGCCGGTCGC
    GCCAATCTGA AAGAAAAAAT GAATGCATCA AACCAACCTC CACACAGAGA CACTGGAAAA
    ACTGTGGAGA ACGTGGAAGA ATACAGCTAT AAGCAGGAGA AAAAGATCCG AGCGGCTCTC
    AGGTCCACGG AGCGGGATCA TAAGAAGAAT GTGCAGTGCT CGTTCATGCT GGACTCAGTT
    GGGGGCTCTC TGCCCAAAAA GTCCATCCCG GATGTGGATC TCAATAAGCC TTACCTCAGT
    CTCGGCTGCA GCAATGCTAA GCTTCCGGTC TCTGTGCCCA TGCCGATAGC CAGAACCGCC
    CGCCAGACTT CCAGGACTGA CTGTCCGGCA GATCGCTTAA AATTTTTTGA AACTCTCCGA
    CTTCTGCTAA AGCTTACCTC AGTCTCAAAG AAGAAGGACA GGGAGCAAAG AGGCCAGGAA
    AGCACATCCG CGTTCTGGTT TAACCGATCT AACGAGCTGA TCTGGTTAGA GCTGCAAGCC
    TGGCACGCCG GCCGGACCAT TAACGACCAG GACCTCTTTT TATACACAGC CCGCCAGGCC
    ATCCCAGACA TTATCAACGA AATCCTTACC TTCAAAGTTA ACTATGGGAG CTTCGCCTTC
    GTTAGAAACG GAGCTAGTCT TAACGGTACT TCAGTAGAAG GGCAGTTCGG AGCCCCTCAC
    GGGACGCAGC CTGCGGGTTA CTCAACCTAT CACGAGCACC TCCAACGCCA GAGGGTGTCC
    TTCGAGCAGG TCAAACGGAT AATGGAGCTG CTGGAGTACA TAGAGGCACT TTATCCATCG
    ATGCAGGCTC TTCAAAAGGA TTATGAGAAG TATGCTGCAA AGGACTTCCA GGACAGGGTG
    CAGGCGCTCT GTTTGTGGTT AAACATCACA AAGGACTTAA ATCAGAAATT AAGGATTATG
    GGCACCGTGT TGGGCATCAA GAATTTATCA GACATTGGCT GGCCGGTGTT TGAAATCCCC
    TCCCCGCGAT CATCCAGGGG CAACGAGCCC GAGGATGAGG CGGAGGACAC AGAAGGAGAG
    CTGAAGGAGC TGGACAGCAG CACGGAGGAG AGTGAGGAAG AGCAGAGCTG CGGCCCGAGG
    GCACCGGAGC CCAGGCCGCC CGCCGACCAC AGCCGCCACG TCCAGTCGCC CCCGGACTGT
    GTCCCGAAGA AGCTCGAGAG GCTGGAGTCG GAGGACGAGT CTGTCGGCTG GGGAGCCCCA
    GACTGGAGCG CCGAGGCAGG CGTTAGTGGA CACTGTCTGA CCTCTATCTA CAGACCCTTT
    GTAGACAAAG CGCTGAAGCA GATGGGCTTA AGGAAGTTGA TTCTAAGGCT TCACAAGCTG
    ATGGACGGTT CCTTGCAGCG GGCACGTATA GCACTAGTTA AGAGCGACGG TCCAGTGGAG
    TTTTCTGGAT TTCCAGATCC CATGTGGGGT TCAGATTATG TGCAGTTGTC CAGGACGCCG
    CCTTCCTCGG AGCAGAAGTG CAGCACCGTG TCCTGGGAGG AGCTGAGGTC CATGGATTTA
    CCCTCCTTCG AGCCTGCCTT CCTGGTCCTC TGTCGAGTCC TTCTGAACGT CATTCACGAA
    TGTCTCAAGT TAAGGCTGGA ACAGAGGCCC GCTGGAGAGC CATCCCTCTT GAGTATTAAG
    CAGCTGGTGA GAGAATGTAA GGAGGTGCTG AAGGGCGGCC TCCTGATGAA GCAGTACTAC
    CAGTTCATGC TGCACGAGGT CCTAGACCAG CTGGAGAAGA CCGACTGCAA CATCGACGCC
    TTCGAGGAGG ACCTGCACAA AATGCTGATG GTATATTTTG ATTACATGAG AAGCTGGATC
    CAAATGCTAC AGGAATTACC TCAAGCATCT CATAGTTTAA AAAATTTGTT AGAAGAAGAA
    TGGAATTTCA CCAAAGAAAT AACCCATTAC ATCCGGGGAG GAGAAGCACA GGCTGGAAAG
    CTTTTCTGTG ACATTGCAGG AATGCTGCTG AAATCAACAG GAAGTTTTCT AGAGTCTGGC
    TTACAGGAGA GCTGTGCGGA ATTCTGGACC AGTGCCGACG ACAGCAGCGC TTCAGATGAA
    ATAAGGAGGT CGGTTATAGA GGTCAGCCGA GCACTGAAGG AGCTCTTCCA CGAGGCCAGG
    GAACGAGCCT CCAAGGCACT GGGGTTTGCC AAGATGCTGA GAAAGGACCT GGAAATAGCT
    GCGGAGTTCA TACTCTCGGC CCCGGTTAGA GACCTTCTGG ACGTTCTGAA AGCAAAGCAG
    TACGTTAAGG TACAGATTCC TGGCTTAGAA AACTTGCAGG TGTTTGTTCC AGACGCTCTT
    GCTGAGGAGA AGAGCATTAT TTTGCAGTTA CTCAATGCAG CTACAGGGAA GGACTGCTCC
    AAAGATTCAG ATGAAGCACT AATTGACGAC TATCTACTTT TGACCAAGCA TGGTGACCGC
    ACTCCAGACT CGGAGGCCAG CTGGGCCGCC TGGGAGGCGC AGCCTGTCAA GGTCGTGCCT
    CCGGTGGAGA CAGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAATCTTCT ACTAGTTGTC
    ATGCACTCTG CGCAGCTCAT AATCCATAGA AAAGCTTTCC AGCAGTCCAT CGAAGGACTC
    ATGACCCTAC GCCAGGAGCA GACTTCCAGT CAGCCCGTCA TCGCCAAAGC TTTGCAGCAG
    CTGAAGAATG ATGCCTTAGA GCTCTGCAAC CGAATAAGTG ATGCCATTGA CCGAGTGGAC
    CACATGTTCA CTTCAGAATT TGATGCTGAA GTTGATGAAT CTGAGTCTGC TACACTGCAG
    CAGTATTACC GAGAAGCCAT GATCCAAGGG TACAATTTCG GGTTCGAGTA TCATAAAGAA
    GTTGTTCGTT TGATGTCTGG CGAGTTTAAA CAGAAGATAG GAGACAAGTA TATAAGCTTT
    GCCCGGAAAT GGATGAATTA CGTCCTGACT AAGTGTGAGA GCGGCAGAGG GACGAGACCC
    AGGTGGGCAA CTCAAGGTTT TGACTTCCTG CAAGCCATCG AGCCTTCGTT CATCTCTGCT
    CTGCCAGAAG ACGACTTCCT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT TGGCCATGTG
    ATAGGAAAGC CGCACAGCCC TATTACAGCC ATTCATCGGA ATAGCCCCCG TCCTGTGAAG
    GTACCTCGAT GCCATAGCGA CCCTCCTAAC CCTCACCTGA TTATCCCAAC TCCAGAGGGA
    TTCAGCGCCC GGAGCGTGCC CGCGGATGTG CGGAGCCTCG GCAGCCCCGC GCCCGCGCCT
    GCCGCCGCTC GTCCTGGCCC CTCGGGCAGC GACCCAGCAC CGCCCAAGCC CATCAGCGGT
    GCCCATGAGA CCAGGGGTTC CAGCGTCCCT GAAAATGACC GCCTGGCTTC CATCGCAGCT
    GAATTGCAGT TCAGGTCGCT GAGCCGCCAC TCCAGCCCCA CCGAGGAGCG CGAGGAACCA
    GCATATCCAA AAGGTGATTC AAGTGGGTCA ACTCGAAGAA GTTGGGAACT TCGGACGCTG
    ATCAGTCAGA CCAAGGATAC CGCTTCTAAG CAAGGACCCA TAGAAGCCAT TCAGAAGTCA
    GTCCGGCTGT TTGAAGAAAA GCGGTATCGA GAAATGAGGA GGAAAAACAT CATTGGTCAA
    GTCTGTGACA CCCCCAAGTC TTACGACAAC GTCATGCACG TTGGCTTGAG GAAGGTGACC
    TTTAAGTGGC AAAGAGGAAA CAAAATCGGG GAAGGGCAGT ACGGGAAGGT GTACACCTGC
    ATCAACGTCG ACACCGGCGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA ACCCAACGAC
    CATAAAACCA TCAAGGAAAC TGCAGACGAG CTGAAAATCT TTGAAGGCAT CAAGCACCCC
    AATCTGGTTC GGTATTTTGG TGTGGAGCTA CATAGAGAGG AGATGTACAT CTTCATGGAG
    TACTGTGACG AGGGCACGTT GGAAGAGGTG TCGCGGCTGG GGCTTCAAGA ACACGTCATC
    AGGCTGTATT CGAAACAGAT TACCGTGGCC ATCAACGTCC TGCACGAGCA TGGCATAGTT
    CACCGCGACG TTAAAGGTGC CAACATCTTC CTGACCTCGT CTGGACTAAT CAAGCTGGGG
    GATTTCGGAT GCTCAGTAAA GCTTAAGAAC AATGCGCAGA CCATGCCTGG TGAAGTGAAC
    AGCACGCTGG GGACAGCAGC GTACATGGCT CCGGAAGTCA TCACTCGCGC CAAAGGAGAA
    GGCCACGGGC GTGCGGCTGA CATCTGGAGC CTGGGGTGTG TTGTCATAGA GATGGTGACT
    GGCAAGAGAC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTACAA AGTGGGAATG
    GGGCACAAGC CGCCGATCCC TGAACGATTA AGCCCCGAAG GGAAGGACTT CCTCTCTCAC
    TGCCTGGAGA GCGAGCCCCG GATGCGGTGG ACAGCCAGCC AGCTCCTCGA CCACTCCTTC
    GTCAAGGTCT GCACGGATGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.