MBD4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD4
Entrez Gene ID	511463
Full Name	methyl-CpG binding domain 4, DNA glycosylase
General protein information	Preferred Names methyl-CpG binding domain 4, DNA glycosylase Names methyl-CpG-binding domain protein 4 methyl-CpG binding domain protein 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002697088.6	XP_002697134.5	methyl-CpG-binding domain protein 4 isoform X4
XM_024982591.1	XP_024838359.1	methyl-CpG-binding domain protein 4 isoform X7
XM_024982587.1	XP_024838355.1	methyl-CpG-binding domain protein 4 isoform X1
NM_001034405.2	NP_001029577.1	methyl-CpG-binding domain protein 4
XM_024982588.1	XP_024838356.1	methyl-CpG-binding domain protein 4 isoform X2
XM_024982590.1	XP_024838358.1	methyl-CpG-binding domain protein 4 isoform X5
XM_024982589.1	XP_024838357.1	methyl-CpG-binding domain protein 4 isoform X3
XM_005223120.4	XP_005223177.1	methyl-CpG-binding domain protein 4 isoform X6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MBD4	XP_001144095.1
Rattus norvegicus (Norway rat)	Mbd4	XP_001059437.3
Homo sapiens (human)	MBD4	NP_003916.1
	MBD4	XP_001116090.2
Bos taurus (cattle)	MBD4	NP_001029577.1
Mus musculus (house mouse)	Mbd4	NP_034904.2
Canis lupus familiaris (dog)	MBD4	XP_003432809.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MBD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39536	NM_001034405.2 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa31780	XM_005223120.4 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa228837	XM_024982587.1 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228838	XM_024982588.1 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228839	XM_024982589.1 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228840	XM_002697088.6 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa228841	XM_024982591.1 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa31779	XM_024982590.1 Latest version!	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD4 ( NM_001034405.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029577.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), mRNA.

Price & Availability↑

CloneID	OBa39536
Clone ID Related Accession (Same CDS sequence)	NM_001034405.2
Accession Version	NM_001034405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103441.1. On Aug 31, 2012 this sequence version replaced NM_001034405.1. ##Evidence-Data-START## Transcript exon combination :: BC103441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGGAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGGTA CACCCTGAAG ACCATAAGTT AAATAAATAT 
CATGACTGGC TTTGGGAAAA TCACGAGAAA TTAAGTCTAT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029577.1
CDS	117..1781
Translation	MESPSLRDCGAAPSVVDRERLVPDRLCDLREEDVALGSERVGEDEKQMVMKSSSECNLPLQEPFASAQSGGNAMTECRKSVPCGWERVVKQRLSGKTAGKYDVHFISPQGQKFRSRVSLVNYLHQSGETSLKPEDFDFSVCPERSIKSECKGRNTAALTFQLQHQSNSSNRNLRTRSMRKKDVFPLPSACLELQDSGELPNLKEDEGVHDIASRKVSKSKRKVTVLKEIQTKKTKPGCRKSLPDPVQSNRESQSEPVAQESHLEGTCCISDARASEQALSVTTEEERLGKKKSSCLGPNGEQIPSGIVSKLCSAKEVEPNNKCEDPLLESEEIRTKVEAAERKEHLHMDISEGGSETDDSSQTQKNPTSAKVSQGDTVPRTQIEKRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWQFLEKYPSAEAARTADWRDVSEFLKPLGLYDLRAKTIIKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS

Target ORF information:

RefSeq Version	NM_001034405.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034405.2

ORF Insert Sequence:

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGGAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGGTA CACCCTGAAG ACCATAAGTT AAATAAATAT 
CATGACTGGC TTTGGGAAAA TCACGAGAAA TTAAGTCTAT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_005223120.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223177.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa31780
Clone ID Related Accession (Same CDS sequence)	XM_005223120.4
Accession Version	XM_005223120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGTA CGAGGCTGCG TTCCGGTCGC TGCTCCGCTG GGTACCGGGG ATACACAGGA 
AGTAAGACAC AGTTCTTACC GTTGGGAGAC TCACAGGCTG TTGGGAAAAA GTATCGGCAT 
CAAGAAAAAT ATGGGTTCTA CCAGTCTAAG TCCCTGTTGG ATGATGAAAA TGCCAGCAAG 
GAGCCGGTGT TCAACCCCGA AGCGGTCATC GCCCACTGCT TCAAACAGTT CAAGCAGGAG 
GACTTCCACC TGCCCAGGAG CCGCCGGCGG GTCATCATCC TGCCACAGAA GGAGGCTCCG 
AAGCCTGTCC GTCCCACGCC CCAACTCCAG ACTCCCCTGG AGCCCACCCC CAGCCCCAAA 
GCCCCGGAAG TCGGGGACAC CCAAGAACAG CCAGAGGATC CGAGGGCGTG GCTCAGACAA 
AGGCTGAAGA TGCGGAAGGA CCTGGAATCG TTTGGCAACG TCGAGAGGTG GCTGCAGAAC 
AAGGCCAACC TCACGCTGTC CAAGGCCAAG GTCCTACGTG ACATCCACAA GAAGCAGCCC 
GTGGCTCGGC TGCCGAGCCA GCTGGCCATG ATCAGGGACA CTGCGAAGAA AAGCCAGCAA 
CCCTCCCGCC GGCAGGTGCC CCAGCTCCGA CTGCCCAAAC CCCCCGCCCT GTCAACCTTG 
TACTCCTACC TGCACTGCCG CAAGATCAAG ATCCTGGAGC TGTTTCACAA GGTGGACCAG 
GGCAAGCACC AGATCTCCCG AGAGGAGTTC ATCGTGGCTC TCAAGGCGAT TGGAGTCCCT 
CTGAAAAACC AGGAGGTGGA GGACATTGTG GTCTACCTCA GCTCCCTGGG GAAGCACAAC 
AGCATCACGA TGGATAACCT GGCCAGCACC TACAAGCAGT GGTCTTTGGC TCGGCAGCAA 
AGCACCCTGA CCATCGCAAC AGAGGATTCC AGATCATCCA ATGACAGCGG CTCCACACAG 
AGTTCATCCC CAAAGCAGAA GGCGAATGTA CCCCCAGAGG CTCCCAAGAT GGACCTGCTG 
ACGGTGCCCG TGGTCGACAC GGAGATGGAG GCACGGCCCA TGACACTGGA GGAGATGGAA 
GATGTGGGCA AGCACTACCG AGAGAGGAAG CGGCAGAACA AGCTGGCCCT CCCCTCCATC 
CAGTACACGG AGCAGTGCCG CCTGGTGCGC AGCGGGGACA AGCACTTTGA CGAGCACTGC 
CTGCCGACCA CAGTCCACGG GGAGATGGGG AGCATCCTCA ACAGGTCCCG CGTGGACACC 
TTCCTGGTCT ACCTGCAGTG CTGGAAGGTC TGCGAGGCCC ACGGCCTCCC GCTGACCGAG 
GACATCCTCA TGAGAGCCCT GATGTACCCA GGGGACAAGA TCATTTTCCA GAAGGATGAG 
ATGCGCCCGA TCCGGCAGCC AGGAGGCTAC TACTCAGACT TCAAGCCCTT CACTGAGAAC 
CTGGCCCTGG TCCGGCCCCA GGGTGTTCCT GTGCCTGTGC CTCAGAAGAA GCCTGACAAG 
AAAATGCCGA AGAAAATCAA GAAAATCAAT TTTAAGGAAT TTGAGGAATT CACCAGGAAG 
CTGAAGGAGA AGAAGCCCAG CAGTCCACAG CTAACCCATC CCAACTTCTT CTGGCCGGGT 
CACCTCCTGG ACAAGCTGCG GCTCTACCTG CCCACTGTGA CCATGGACCG GAGCCTGGCC 
ATCTTCAGCT GTGTCCAACA GCGGCCCCAC GTCTACCCGG CCACCTACCA CTCCGACTGC 
TGGTGGCCCA TTAAGGACGT GAATTACGTG ACCTACGCCT ATTACGATGC CCACAAGATC 
TATCACATCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223177.1
CDS	819..2633
Translation	MKSTRLRSGRCSAGYRGYTGSKTQFLPLGDSQAVGKKYRHQEKYGFYQSKSLLDDENASKEPVFNPEAVIAHCFKQFKQEDFHLPRSRRRVIILPQKEAPKPVRPTPQLQTPLEPTPSPKAPEVGDTQEQPEDPRAWLRQRLKMRKDLESFGNVERWLQNKANLTLSKAKVLRDIHKKQPVARLPSQLAMIRDTAKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKLALPSIQYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_005223120.4
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223120.4

ORF Insert Sequence:

ATGAAGAGTA CGAGGCTGCG TTCCGGTCGC TGCTCCGCTG GGTACCGGGG ATACACAGGA 
AGTAAGACAC AGTTCTTACC GTTGGGAGAC TCACAGGCTG TTGGGAAAAA GTATCGGCAT 
CAAGAAAAAT ATGGGTTCTA CCAGTCTAAG TCCCTGTTGG ATGATGAAAA TGCCAGCAAG 
GAGCCGGTGT TCAACCCCGA AGCGGTCATC GCCCACTGCT TCAAACAGTT CAAGCAGGAG 
GACTTCCACC TGCCCAGGAG CCGCCGGCGG GTCATCATCC TGCCACAGAA GGAGGCTCCG 
AAGCCTGTCC GTCCCACGCC CCAACTCCAG ACTCCCCTGG AGCCCACCCC CAGCCCCAAA 
GCCCCGGAAG TCGGGGACAC CCAAGAACAG CCAGAGGATC CGAGGGCGTG GCTCAGACAA 
AGGCTGAAGA TGCGGAAGGA CCTGGAATCG TTTGGCAACG TCGAGAGGTG GCTGCAGAAC 
AAGGCCAACC TCACGCTGTC CAAGGCCAAG GTCCTACGTG ACATCCACAA GAAGCAGCCC 
GTGGCTCGGC TGCCGAGCCA GCTGGCCATG ATCAGGGACA CTGCGAAGAA AAGCCAGCAA 
CCCTCCCGCC GGCAGGTGCC CCAGCTCCGA CTGCCCAAAC CCCCCGCCCT GTCAACCTTG 
TACTCCTACC TGCACTGCCG CAAGATCAAG ATCCTGGAGC TGTTTCACAA GGTGGACCAG 
GGCAAGCACC AGATCTCCCG AGAGGAGTTC ATCGTGGCTC TCAAGGCGAT TGGAGTCCCT 
CTGAAAAACC AGGAGGTGGA GGACATTGTG GTCTACCTCA GCTCCCTGGG GAAGCACAAC 
AGCATCACGA TGGATAACCT GGCCAGCACC TACAAGCAGT GGTCTTTGGC TCGGCAGCAA 
AGCACCCTGA CCATCGCAAC AGAGGATTCC AGATCATCCA ATGACAGCGG CTCCACACAG 
AGTTCATCCC CAAAGCAGAA GGCGAATGTA CCCCCAGAGG CTCCCAAGAT GGACCTGCTG 
ACGGTGCCCG TGGTCGACAC GGAGATGGAG GCACGGCCCA TGACACTGGA GGAGATGGAA 
GATGTGGGCA AGCACTACCG AGAGAGGAAG CGGCAGAACA AGCTGGCCCT CCCCTCCATC 
CAGTACACGG AGCAGTGCCG CCTGGTGCGC AGCGGGGACA AGCACTTTGA CGAGCACTGC 
CTGCCGACCA CAGTCCACGG GGAGATGGGG AGCATCCTCA ACAGGTCCCG CGTGGACACC 
TTCCTGGTCT ACCTGCAGTG CTGGAAGGTC TGCGAGGCCC ACGGCCTCCC GCTGACCGAG 
GACATCCTCA TGAGAGCCCT GATGTACCCA GGGGACAAGA TCATTTTCCA GAAGGATGAG 
ATGCGCCCGA TCCGGCAGCC AGGAGGCTAC TACTCAGACT TCAAGCCCTT CACTGAGAAC 
CTGGCCCTGG TCCGGCCCCA GGGTGTTCCT GTGCCTGTGC CTCAGAAGAA GCCTGACAAG 
AAAATGCCGA AGAAAATCAA GAAAATCAAT TTTAAGGAAT TTGAGGAATT CACCAGGAAG 
CTGAAGGAGA AGAAGCCCAG CAGTCCACAG CTAACCCATC CCAACTTCTT CTGGCCGGGT 
CACCTCCTGG ACAAGCTGCG GCTCTACCTG CCCACTGTGA CCATGGACCG GAGCCTGGCC 
ATCTTCAGCT GTGTCCAACA GCGGCCCCAC GTCTACCCGG CCACCTACCA CTCCGACTGC 
TGGTGGCCCA TTAAGGACGT GAATTACGTG ACCTACGCCT ATTACGATGC CCACAAGATC 
TATCACATCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_024982587.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838355.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228837
Clone ID Related Accession (Same CDS sequence)	XM_024982587.1
Accession Version	XM_024982587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGAAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGAAG AAAAGCCAGC AACCCTCCCG CCGGCAGGTG 
CCCCAGCTCC GACTGCCCAA ACCCCCCGCC CTGTCAACCT TGTACTCCTA CCTGCACTGC 
CGCAAGATCA AGATCCTGGA GCTGTTTCAC AAGGTGGACC AGGGCAAGCA CCAGATCTCC 
CGAGAGGAGT TCATCGTGGC TCTCAAGGCG ATTGGAGTCC CTCTGAAAAA CCAGGAGGTG 
GAGGACATTG TGGTCTACCT CAGCTCCCTG GGGAAGCACA ACAGCATCAC GATGGATAAC 
CTGGCCAGCA CCTACAAGCA GTGGTCTTTG GCTCGGCAGC AAAGCACCCT GACCATCGCA 
ACAGAGGATT CCAGATCATC CAATGACAGC GGCTCCACAC AGAGTTCATC CCCAAAGCAG 
AAGGCGAATG TACCCCCAGA GGCTCCCAAG ATGGACCTGC TGACGGTGCC CGTGGTCGAC 
ACGGAGATGG AGGCACGGCC CATGACACTG GAGGAGATGG AAGATGTGGG CAAGCACTAC 
CGAGAGAGGA AGCGGCAGAA CAAGCTGGCC CTCCCCTCCA TCCAGTACAC GGAGCAGTGC 
CGCCTGGTGC GCAGCGGGGA CAAGCACTTT GACGAGCACT GCCTGCCGAC CACAGTCCAC 
GGGGAGATGG GGAGCATCCT CAACAGGTCC CGCGTGGACA CCTTCCTGGT CTACCTGCAG 
TGCTGGAAGG TCTGCGAGGC CCACGGCCTC CCGCTGACCG AGGACATCCT CATGAGAGCC 
CTGATGTACC CAGGGGACAA GATCATTTTC CAGAAGGATG AGATGCGCCC GATCCGGCAG 
CCAGGAGGCT ACTACTCAGA CTTCAAGCCC TTCACTGAGA ACCTGGCCCT GGTCCGGCCC 
CAGGGTGTTC CTGTGCCTGT GCCTCAGAAG AAGCCTGACA AGAAAATGCC GAAGAAAATC 
AAGAAAATCA ATTTTAAGGA ATTTGAGGAA TTCACCAGGA AGCTGAAGGA GAAGAAGCCC 
AGCAGTCCAC AGCTAACCCA TCCCAACTTC TTCTGGCCGG GTCACCTCCT GGACAAGCTG 
CGGCTCTACC TGCCCACTGT GACCATGGAC CGGAGCCTGG CCATCTTCAG CTGTGTCCAA 
CAGCGGCCCC ACGTCTACCC GGCCACCTAC CACTCCGACT GCTGGTGGCC CATTAAGGAC 
GTGAATTACG TGACCTACGC CTATTACGAT GCCCACAAGA TCTATCACAT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838355.1
CDS	208..3024
Translation	MESPSLRDCGAAPSVVDRERLVPDRLCDLREEDVALGSERVGEDEKQMVMKSSSECNLPLQEPFASAQSGGNAMTECRKSVPCGWERVVKQRLSGKTAGKYDVHFISPQGQKFRSRVSLVNYLHQSGETSLKPEDFDFSVCPERSIKSECKGRNTAALTFQLQHQSNSSNRNLRTRSMRKKDVFPLPSACLELQDSGELPNLKEDEGVHDIASRKVSKSKRKVTVLKEIQTKKTKPGCRKSLPDPVQSNRESQSEPVAQESHLEGTCCISDARASEQALSVTTEEERLGKKKSSCLGPNGEQIPSGIVSKLCSAKEVEPNNKCEDPLLESEEIRTKVEAAERKEHLHMDISEGGSETDDSSQTQKNPTSAKVSQEDTVPRTQIEKRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWQFLEKYPSAEAARTADWRDVSEFLKPLGLYDLRAKTIIKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKLALPSIQYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_024982587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982587.1

ORF Insert Sequence:

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGAAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGAAG AAAAGCCAGC AACCCTCCCG CCGGCAGGTG 
CCCCAGCTCC GACTGCCCAA ACCCCCCGCC CTGTCAACCT TGTACTCCTA CCTGCACTGC 
CGCAAGATCA AGATCCTGGA GCTGTTTCAC AAGGTGGACC AGGGCAAGCA CCAGATCTCC 
CGAGAGGAGT TCATCGTGGC TCTCAAGGCG ATTGGAGTCC CTCTGAAAAA CCAGGAGGTG 
GAGGACATTG TGGTCTACCT CAGCTCCCTG GGGAAGCACA ACAGCATCAC GATGGATAAC 
CTGGCCAGCA CCTACAAGCA GTGGTCTTTG GCTCGGCAGC AAAGCACCCT GACCATCGCA 
ACAGAGGATT CCAGATCATC CAATGACAGC GGCTCCACAC AGAGTTCATC CCCAAAGCAG 
AAGGCGAATG TACCCCCAGA GGCTCCCAAG ATGGACCTGC TGACGGTGCC CGTGGTCGAC 
ACGGAGATGG AGGCACGGCC CATGACACTG GAGGAGATGG AAGATGTGGG CAAGCACTAC 
CGAGAGAGGA AGCGGCAGAA CAAGCTGGCC CTCCCCTCCA TCCAGTACAC GGAGCAGTGC 
CGCCTGGTGC GCAGCGGGGA CAAGCACTTT GACGAGCACT GCCTGCCGAC CACAGTCCAC 
GGGGAGATGG GGAGCATCCT CAACAGGTCC CGCGTGGACA CCTTCCTGGT CTACCTGCAG 
TGCTGGAAGG TCTGCGAGGC CCACGGCCTC CCGCTGACCG AGGACATCCT CATGAGAGCC 
CTGATGTACC CAGGGGACAA GATCATTTTC CAGAAGGATG AGATGCGCCC GATCCGGCAG 
CCAGGAGGCT ACTACTCAGA CTTCAAGCCC TTCACTGAGA ACCTGGCCCT GGTCCGGCCC 
CAGGGTGTTC CTGTGCCTGT GCCTCAGAAG AAGCCTGACA AGAAAATGCC GAAGAAAATC 
AAGAAAATCA ATTTTAAGGA ATTTGAGGAA TTCACCAGGA AGCTGAAGGA GAAGAAGCCC 
AGCAGTCCAC AGCTAACCCA TCCCAACTTC TTCTGGCCGG GTCACCTCCT GGACAAGCTG 
CGGCTCTACC TGCCCACTGT GACCATGGAC CGGAGCCTGG CCATCTTCAG CTGTGTCCAA 
CAGCGGCCCC ACGTCTACCC GGCCACCTAC CACTCCGACT GCTGGTGGCC CATTAAGGAC 
GTGAATTACG TGACCTACGC CTATTACGAT GCCCACAAGA TCTATCACAT CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_024982588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838356.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228838
Clone ID Related Accession (Same CDS sequence)	XM_024982588.1
Accession Version	XM_024982588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGACACCGT CCCACGGACA 
CAAATAGAAA AAAGGAAAAC AAGCCTGTAT TTTTCCAGCA AATACAACAA AGAAGCTCTT 
AGCCCCCCAC GACGCAAGGC CTTTAAGAAG TGGACACCTC CGCGGTCACC TTTTAATCTC 
GTCCAGGAAA CTCTTTTTCA TGATCCGTGG AAGCTCCTCA TTGCAACTAT ATTTCTCAAT 
AGGACCTCAG GCAAAATGGC CATCCCTGTG CTCTGGCAGT TTCTGGAGAA GTACCCTTCT 
GCTGAGGCTG CAAGGACCGC AGACTGGAGA GATGTTTCAG AATTTCTTAA ACCTCTTGGT 
CTCTACGATC TTCGAGCAAA AACCATTATC AAGTTCTCCG ATGAATATCT GACAAAGCAG 
TGGAAGTATC CGATCGAGCT TCACGGGATT GGCAAATATG GCAACGACTC GTACCGAATT 
TTTTGCGTCA ATGAGTGGAA GCAGAAGAAA AGCCAGCAAC CCTCCCGCCG GCAGGTGCCC 
CAGCTCCGAC TGCCCAAACC CCCCGCCCTG TCAACCTTGT ACTCCTACCT GCACTGCCGC 
AAGATCAAGA TCCTGGAGCT GTTTCACAAG GTGGACCAGG GCAAGCACCA GATCTCCCGA 
GAGGAGTTCA TCGTGGCTCT CAAGGCGATT GGAGTCCCTC TGAAAAACCA GGAGGTGGAG 
GACATTGTGG TCTACCTCAG CTCCCTGGGG AAGCACAACA GCATCACGAT GGATAACCTG 
GCCAGCACCT ACAAGCAGTG GTCTTTGGCT CGGCAGCAAA GCACCCTGAC CATCGCAACA 
GAGGATTCCA GATCATCCAA TGACAGCGGC TCCACACAGA GTTCATCCCC AAAGCAGAAG 
GCGAATGTAC CCCCAGAGGC TCCCAAGATG GACCTGCTGA CGGTGCCCGT GGTCGACACG 
GAGATGGAGG CACGGCCCAT GACACTGGAG GAGATGGAAG ATGTGGGCAA GCACTACCGA 
GAGAGGAAGC GGCAGAACAA GCTGGCCCTC CCCTCCATCC AGTACACGGA GCAGTGCCGC 
CTGGTGCGCA GCGGGGACAA GCACTTTGAC GAGCACTGCC TGCCGACCAC AGTCCACGGG 
GAGATGGGGA GCATCCTCAA CAGGTCCCGC GTGGACACCT TCCTGGTCTA CCTGCAGTGC 
TGGAAGGTCT GCGAGGCCCA CGGCCTCCCG CTGACCGAGG ACATCCTCAT GAGAGCCCTG 
ATGTACCCAG GGGACAAGAT CATTTTCCAG AAGGATGAGA TGCGCCCGAT CCGGCAGCCA 
GGAGGCTACT ACTCAGACTT CAAGCCCTTC ACTGAGAACC TGGCCCTGGT CCGGCCCCAG 
GGTGTTCCTG TGCCTGTGCC TCAGAAGAAG CCTGACAAGA AAATGCCGAA GAAAATCAAG 
AAAATCAATT TTAAGGAATT TGAGGAATTC ACCAGGAAGC TGAAGGAGAA GAAGCCCAGC 
AGTCCACAGC TAACCCATCC CAACTTCTTC TGGCCGGGTC ACCTCCTGGA CAAGCTGCGG 
CTCTACCTGC CCACTGTGAC CATGGACCGG AGCCTGGCCA TCTTCAGCTG TGTCCAACAG 
CGGCCCCACG TCTACCCGGC CACCTACCAC TCCGACTGCT GGTGGCCCAT TAAGGACGTG 
AATTACGTGA CCTACGCCTA TTACGATGCC CACAAGATCT ATCACATCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838356.1
CDS	209..3022
Translation	MESPSLRDCGAAPSVVDRERLVPDRLCDLREEDVALGSERVGEDEKQMVMKSSSECNLPLQEPFASAQSGGNAMTECRKSVPCGWERVVKQRLSGKTAGKYDVHFISPQGQKFRSRVSLVNYLHQSGETSLKPEDFDFSVCPERSIKSECKGRNTAALTFQLQHQSNSSNRNLRTRSMRKKDVFPLPSACLELQDSGELPNLKEDEGVHDIASRKVSKSKRKVTVLKEIQTKKTKPGCRKSLPDPVQSNRESQSEPVAQESHLEGTCCISDARASEQALSVTTEEERLGKKKSSCLGPNGEQIPSGIVSKLCSAKEVEPNNKCEDPLLESEEIRTKVEAAERKEHLHMDISEGGSETDDSSQTQKNPTSAKVSQDTVPRTQIEKRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWQFLEKYPSAEAARTADWRDVSEFLKPLGLYDLRAKTIIKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKLALPSIQYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_024982588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982588.1

ORF Insert Sequence:

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGACACCGT CCCACGGACA 
CAAATAGAAA AAAGGAAAAC AAGCCTGTAT TTTTCCAGCA AATACAACAA AGAAGCTCTT 
AGCCCCCCAC GACGCAAGGC CTTTAAGAAG TGGACACCTC CGCGGTCACC TTTTAATCTC 
GTCCAGGAAA CTCTTTTTCA TGATCCGTGG AAGCTCCTCA TTGCAACTAT ATTTCTCAAT 
AGGACCTCAG GCAAAATGGC CATCCCTGTG CTCTGGCAGT TTCTGGAGAA GTACCCTTCT 
GCTGAGGCTG CAAGGACCGC AGACTGGAGA GATGTTTCAG AATTTCTTAA ACCTCTTGGT 
CTCTACGATC TTCGAGCAAA AACCATTATC AAGTTCTCCG ATGAATATCT GACAAAGCAG 
TGGAAGTATC CGATCGAGCT TCACGGGATT GGCAAATATG GCAACGACTC GTACCGAATT 
TTTTGCGTCA ATGAGTGGAA GCAGAAGAAA AGCCAGCAAC CCTCCCGCCG GCAGGTGCCC 
CAGCTCCGAC TGCCCAAACC CCCCGCCCTG TCAACCTTGT ACTCCTACCT GCACTGCCGC 
AAGATCAAGA TCCTGGAGCT GTTTCACAAG GTGGACCAGG GCAAGCACCA GATCTCCCGA 
GAGGAGTTCA TCGTGGCTCT CAAGGCGATT GGAGTCCCTC TGAAAAACCA GGAGGTGGAG 
GACATTGTGG TCTACCTCAG CTCCCTGGGG AAGCACAACA GCATCACGAT GGATAACCTG 
GCCAGCACCT ACAAGCAGTG GTCTTTGGCT CGGCAGCAAA GCACCCTGAC CATCGCAACA 
GAGGATTCCA GATCATCCAA TGACAGCGGC TCCACACAGA GTTCATCCCC AAAGCAGAAG 
GCGAATGTAC CCCCAGAGGC TCCCAAGATG GACCTGCTGA CGGTGCCCGT GGTCGACACG 
GAGATGGAGG CACGGCCCAT GACACTGGAG GAGATGGAAG ATGTGGGCAA GCACTACCGA 
GAGAGGAAGC GGCAGAACAA GCTGGCCCTC CCCTCCATCC AGTACACGGA GCAGTGCCGC 
CTGGTGCGCA GCGGGGACAA GCACTTTGAC GAGCACTGCC TGCCGACCAC AGTCCACGGG 
GAGATGGGGA GCATCCTCAA CAGGTCCCGC GTGGACACCT TCCTGGTCTA CCTGCAGTGC 
TGGAAGGTCT GCGAGGCCCA CGGCCTCCCG CTGACCGAGG ACATCCTCAT GAGAGCCCTG 
ATGTACCCAG GGGACAAGAT CATTTTCCAG AAGGATGAGA TGCGCCCGAT CCGGCAGCCA 
GGAGGCTACT ACTCAGACTT CAAGCCCTTC ACTGAGAACC TGGCCCTGGT CCGGCCCCAG 
GGTGTTCCTG TGCCTGTGCC TCAGAAGAAG CCTGACAAGA AAATGCCGAA GAAAATCAAG 
AAAATCAATT TTAAGGAATT TGAGGAATTC ACCAGGAAGC TGAAGGAGAA GAAGCCCAGC 
AGTCCACAGC TAACCCATCC CAACTTCTTC TGGCCGGGTC ACCTCCTGGA CAAGCTGCGG 
CTCTACCTGC CCACTGTGAC CATGGACCGG AGCCTGGCCA TCTTCAGCTG TGTCCAACAG 
CGGCCCCACG TCTACCCGGC CACCTACCAC TCCGACTGCT GGTGGCCCAT TAAGGACGTG 
AATTACGTGA CCTACGCCTA TTACGATGCC CACAAGATCT ATCACATCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_024982589.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838357.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228839
Clone ID Related Accession (Same CDS sequence)	XM_024982589.1
Accession Version	XM_024982589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGAAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGAAG AAAAGCCAGC AACCCTCCCG CCGGCAGGTG 
CCCCAGCTCC GACTGCCCAA ACCCCCCGCC CTGTCAACCT TGTACTCCTA CCTGCACTGC 
CGCAAGATCA AGATCCTGGA GCTGTTTCAC AAGGTGGACC AGGGCAAGCA CCAGATCTCC 
CGAGAGGAGT TCATCGTGGC TCTCAAGGCG ATTGGAGTCC CTCTGAAAAA CCAGGAGGTG 
GAGGACATTG TGGTCTACCT CAGCTCCCTG GGGAAGCACA ACAGCATCAC GATGGATAAC 
CTGGCCAGCA CCTACAAGCA GTGGTCTTTG GCTCGGCAGC AAAGCACCCT GACCATCGCA 
ACAGAGGATT CCAGATCATC CAATGACAGC GGCTCCACAC AGAGTTCATC CCCAAAGCAG 
AAGGCGAATG TACCCCCAGA GGCTCCCAAG ATGGACCTGC TGACGGTGCC CGTGGTCGAC 
ACGGAGATGG AGGCACGGCC CATGACACTG GAGGAGATGG AAGATGTGGG CAAGCACTAC 
CGAGAGAGGA AGCGGCAGAA CAAGTACACG GAGCAGTGCC GCCTGGTGCG CAGCGGGGAC 
AAGCACTTTG ACGAGCACTG CCTGCCGACC ACAGTCCACG GGGAGATGGG GAGCATCCTC 
AACAGGTCCC GCGTGGACAC CTTCCTGGTC TACCTGCAGT GCTGGAAGGT CTGCGAGGCC 
CACGGCCTCC CGCTGACCGA GGACATCCTC ATGAGAGCCC TGATGTACCC AGGGGACAAG 
ATCATTTTCC AGAAGGATGA GATGCGCCCG ATCCGGCAGC CAGGAGGCTA CTACTCAGAC 
TTCAAGCCCT TCACTGAGAA CCTGGCCCTG GTCCGGCCCC AGGGTGTTCC TGTGCCTGTG 
CCTCAGAAGA AGCCTGACAA GAAAATGCCG AAGAAAATCA AGAAAATCAA TTTTAAGGAA 
TTTGAGGAAT TCACCAGGAA GCTGAAGGAG AAGAAGCCCA GCAGTCCACA GCTAACCCAT 
CCCAACTTCT TCTGGCCGGG TCACCTCCTG GACAAGCTGC GGCTCTACCT GCCCACTGTG 
ACCATGGACC GGAGCCTGGC CATCTTCAGC TGTGTCCAAC AGCGGCCCCA CGTCTACCCG 
GCCACCTACC ACTCCGACTG CTGGTGGCCC ATTAAGGACG TGAATTACGT GACCTACGCC 
TATTACGATG CCCACAAGAT CTATCACATC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838357.1
CDS	208..3003
Translation	MESPSLRDCGAAPSVVDRERLVPDRLCDLREEDVALGSERVGEDEKQMVMKSSSECNLPLQEPFASAQSGGNAMTECRKSVPCGWERVVKQRLSGKTAGKYDVHFISPQGQKFRSRVSLVNYLHQSGETSLKPEDFDFSVCPERSIKSECKGRNTAALTFQLQHQSNSSNRNLRTRSMRKKDVFPLPSACLELQDSGELPNLKEDEGVHDIASRKVSKSKRKVTVLKEIQTKKTKPGCRKSLPDPVQSNRESQSEPVAQESHLEGTCCISDARASEQALSVTTEEERLGKKKSSCLGPNGEQIPSGIVSKLCSAKEVEPNNKCEDPLLESEEIRTKVEAAERKEHLHMDISEGGSETDDSSQTQKNPTSAKVSQEDTVPRTQIEKRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWQFLEKYPSAEAARTADWRDVSEFLKPLGLYDLRAKTIIKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_024982589.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982589.1

ORF Insert Sequence:

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGAAGACAC CGTCCCACGG 
ACACAAATAG AAAAAAGGAA AACAAGCCTG TATTTTTCCA GCAAATACAA CAAAGAAGCT 
CTTAGCCCCC CACGACGCAA GGCCTTTAAG AAGTGGACAC CTCCGCGGTC ACCTTTTAAT 
CTCGTCCAGG AAACTCTTTT TCATGATCCG TGGAAGCTCC TCATTGCAAC TATATTTCTC 
AATAGGACCT CAGGCAAAAT GGCCATCCCT GTGCTCTGGC AGTTTCTGGA GAAGTACCCT 
TCTGCTGAGG CTGCAAGGAC CGCAGACTGG AGAGATGTTT CAGAATTTCT TAAACCTCTT 
GGTCTCTACG ATCTTCGAGC AAAAACCATT ATCAAGTTCT CCGATGAATA TCTGACAAAG 
CAGTGGAAGT ATCCGATCGA GCTTCACGGG ATTGGCAAAT ATGGCAACGA CTCGTACCGA 
ATTTTTTGCG TCAATGAGTG GAAGCAGAAG AAAAGCCAGC AACCCTCCCG CCGGCAGGTG 
CCCCAGCTCC GACTGCCCAA ACCCCCCGCC CTGTCAACCT TGTACTCCTA CCTGCACTGC 
CGCAAGATCA AGATCCTGGA GCTGTTTCAC AAGGTGGACC AGGGCAAGCA CCAGATCTCC 
CGAGAGGAGT TCATCGTGGC TCTCAAGGCG ATTGGAGTCC CTCTGAAAAA CCAGGAGGTG 
GAGGACATTG TGGTCTACCT CAGCTCCCTG GGGAAGCACA ACAGCATCAC GATGGATAAC 
CTGGCCAGCA CCTACAAGCA GTGGTCTTTG GCTCGGCAGC AAAGCACCCT GACCATCGCA 
ACAGAGGATT CCAGATCATC CAATGACAGC GGCTCCACAC AGAGTTCATC CCCAAAGCAG 
AAGGCGAATG TACCCCCAGA GGCTCCCAAG ATGGACCTGC TGACGGTGCC CGTGGTCGAC 
ACGGAGATGG AGGCACGGCC CATGACACTG GAGGAGATGG AAGATGTGGG CAAGCACTAC 
CGAGAGAGGA AGCGGCAGAA CAAGTACACG GAGCAGTGCC GCCTGGTGCG CAGCGGGGAC 
AAGCACTTTG ACGAGCACTG CCTGCCGACC ACAGTCCACG GGGAGATGGG GAGCATCCTC 
AACAGGTCCC GCGTGGACAC CTTCCTGGTC TACCTGCAGT GCTGGAAGGT CTGCGAGGCC 
CACGGCCTCC CGCTGACCGA GGACATCCTC ATGAGAGCCC TGATGTACCC AGGGGACAAG 
ATCATTTTCC AGAAGGATGA GATGCGCCCG ATCCGGCAGC CAGGAGGCTA CTACTCAGAC 
TTCAAGCCCT TCACTGAGAA CCTGGCCCTG GTCCGGCCCC AGGGTGTTCC TGTGCCTGTG 
CCTCAGAAGA AGCCTGACAA GAAAATGCCG AAGAAAATCA AGAAAATCAA TTTTAAGGAA 
TTTGAGGAAT TCACCAGGAA GCTGAAGGAG AAGAAGCCCA GCAGTCCACA GCTAACCCAT 
CCCAACTTCT TCTGGCCGGG TCACCTCCTG GACAAGCTGC GGCTCTACCT GCCCACTGTG 
ACCATGGACC GGAGCCTGGC CATCTTCAGC TGTGTCCAAC AGCGGCCCCA CGTCTACCCG 
GCCACCTACC ACTCCGACTG CTGGTGGCCC ATTAAGGACG TGAATTACGT GACCTACGCC 
TATTACGATG CCCACAAGAT CTATCACATC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_002697088.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697134.5 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228840
Clone ID Related Accession (Same CDS sequence)	XM_002697088.6
Accession Version	XM_002697088.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697088.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG TTGCGTCCAA GCTCCGCAGG TCTCGCGTCT CTTGCAAACT TGGTTATTAC 
TGCTTTTCAG ATACCCCATA CTCCCGCGCA AGCACACACA CACACACACA CATTCACACG 
CGCTCTTCAG CGCCGGCTCA CGCACATATT CACACGCGCT CTTCAGTGCC GGCTCCCAGC 
GCCGGGACCA TGGACGACTC TAATGAAGAG TACGAGGCTG CGTTCCGGTC GCTGCTCCGG 
TTCTACCAGT CTAAGTCCCT GTTGGATGAT GAAAATGCCA GCAAGGAGCC GGTGTTCAAC 
CCCGAAGCGG TCATCGCCCA CTGCTTCAAA CAGTTCAAGC AGGAGGACTT CCACCTGCCC 
AGGAGCCGCC GGCGGGTCAT CATCCTGCCA CAGAAGGAGG CTCCGAAGCC TGTCCGTCCC 
ACGCCCCAAC TCCAGACTCC CCTGGAGCCC ACCCCCAGCC CCAAAGCCCC GGAAGTCGGG 
GACACCCAAG AACAGCCAGA GGATCCGAGG GCGTGGCTCA GACAAAGGCT GAAGATGCGG 
AAGGACCTGG AATCGTTTGG CAACGTCGAG AGGTGGCTGC AGAACAAGGC CAACCTCACG 
CTGTCCAAGG CCAAGGTCCT ACGTGACATC CACAAGAAGC AGCCCGTGGC TCGGCTGCCG 
AGCCAGCTGG CCATGATCAG GGACACTGCG AAGAAAAGCC AGCAACCCTC CCGCCGGCAG 
GTGCCCCAGC TCCGACTGCC CAAACCCCCC GCCCTGTCAA CCTTGTACTC CTACCTGCAC 
TGCCGCAAGA TCAAGATCCT GGAGCTGTTT CACAAGGTGG ACCAGGGCAA GCACCAGATC 
TCCCGAGAGG AGTTCATCGT GGCTCTCAAG GCGATTGGAG TCCCTCTGAA AAACCAGGAG 
GTGGAGGACA TTGTGGTCTA CCTCAGCTCC CTGGGGAAGC ACAACAGCAT CACGATGGAT 
AACCTGGCCA GCACCTACAA GCAGTGGTCT TTGGCTCGGC AGCAAAGCAC CCTGACCATC 
GCAACAGAGG ATTCCAGATC ATCCAATGAC AGCGGCTCCA CACAGAGTTC ATCCCCAAAG 
CAGAAGGCGA ATGTACCCCC AGAGGCTCCC AAGATGGACC TGCTGACGGT GCCCGTGGTC 
GACACGGAGA TGGAGGCACG GCCCATGACA CTGGAGGAGA TGGAAGATGT GGGCAAGCAC 
TACCGAGAGA GGAAGCGGCA GAACAAGCTG GCCCTCCCCT CCATCCAGTA CACGGAGCAG 
TGCCGCCTGG TGCGCAGCGG GGACAAGCAC TTTGACGAGC ACTGCCTGCC GACCACAGTC 
CACGGGGAGA TGGGGAGCAT CCTCAACAGG TCCCGCGTGG ACACCTTCCT GGTCTACCTG 
CAGTGCTGGA AGGTCTGCGA GGCCCACGGC CTCCCGCTGA CCGAGGACAT CCTCATGAGA 
GCCCTGATGT ACCCAGGGGA CAAGATCATT TTCCAGAAGG ATGAGATGCG CCCGATCCGG 
CAGCCAGGAG GCTACTACTC AGACTTCAAG CCCTTCACTG AGAACCTGGC CCTGGTCCGG 
CCCCAGGGTG TTCCTGTGCC TGTGCCTCAG AAGAAGCCTG ACAAGAAAAT GCCGAAGAAA 
ATCAAGAAAA TCAATTTTAA GGAATTTGAG GAATTCACCA GGAAGCTGAA GGAGAAGAAG 
CCCAGCAGTC CACAGCTAAC CCATCCCAAC TTCTTCTGGC CGGGTCACCT CCTGGACAAG 
CTGCGGCTCT ACCTGCCCAC TGTGACCATG GACCGGAGCC TGGCCATCTT CAGCTGTGTC 
CAACAGCGGC CCCACGTCTA CCCGGCCACC TACCACTCCG ACTGCTGGTG GCCCATTAAG 
GACGTGAATT ACGTGACCTA CGCCTATTAC GATGCCCACA AGATCTATCA CATCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697134.5
CDS	615..2534
Translation	MATVASKLRRSRVSCKLGYYCFSDTPYSRASTHTHTHIHTRSSAPAHAHIHTRSSVPAPSAGTMDDSNEEYEAAFRSLLRFYQSKSLLDDENASKEPVFNPEAVIAHCFKQFKQEDFHLPRSRRRVIILPQKEAPKPVRPTPQLQTPLEPTPSPKAPEVGDTQEQPEDPRAWLRQRLKMRKDLESFGNVERWLQNKANLTLSKAKVLRDIHKKQPVARLPSQLAMIRDTAKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKLALPSIQYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_002697088.6
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697088.6

ORF Insert Sequence:

ATGGCAACAG TTGCGTCCAA GCTCCGCAGG TCTCGCGTCT CTTGCAAACT TGGTTATTAC 
TGCTTTTCAG ATACCCCATA CTCCCGCGCA AGCACACACA CACACACACA CATTCACACG 
CGCTCTTCAG CGCCGGCTCA CGCACATATT CACACGCGCT CTTCAGTGCC GGCTCCCAGC 
GCCGGGACCA TGGACGACTC TAATGAAGAG TACGAGGCTG CGTTCCGGTC GCTGCTCCGG 
TTCTACCAGT CTAAGTCCCT GTTGGATGAT GAAAATGCCA GCAAGGAGCC GGTGTTCAAC 
CCCGAAGCGG TCATCGCCCA CTGCTTCAAA CAGTTCAAGC AGGAGGACTT CCACCTGCCC 
AGGAGCCGCC GGCGGGTCAT CATCCTGCCA CAGAAGGAGG CTCCGAAGCC TGTCCGTCCC 
ACGCCCCAAC TCCAGACTCC CCTGGAGCCC ACCCCCAGCC CCAAAGCCCC GGAAGTCGGG 
GACACCCAAG AACAGCCAGA GGATCCGAGG GCGTGGCTCA GACAAAGGCT GAAGATGCGG 
AAGGACCTGG AATCGTTTGG CAACGTCGAG AGGTGGCTGC AGAACAAGGC CAACCTCACG 
CTGTCCAAGG CCAAGGTCCT ACGTGACATC CACAAGAAGC AGCCCGTGGC TCGGCTGCCG 
AGCCAGCTGG CCATGATCAG GGACACTGCG AAGAAAAGCC AGCAACCCTC CCGCCGGCAG 
GTGCCCCAGC TCCGACTGCC CAAACCCCCC GCCCTGTCAA CCTTGTACTC CTACCTGCAC 
TGCCGCAAGA TCAAGATCCT GGAGCTGTTT CACAAGGTGG ACCAGGGCAA GCACCAGATC 
TCCCGAGAGG AGTTCATCGT GGCTCTCAAG GCGATTGGAG TCCCTCTGAA AAACCAGGAG 
GTGGAGGACA TTGTGGTCTA CCTCAGCTCC CTGGGGAAGC ACAACAGCAT CACGATGGAT 
AACCTGGCCA GCACCTACAA GCAGTGGTCT TTGGCTCGGC AGCAAAGCAC CCTGACCATC 
GCAACAGAGG ATTCCAGATC ATCCAATGAC AGCGGCTCCA CACAGAGTTC ATCCCCAAAG 
CAGAAGGCGA ATGTACCCCC AGAGGCTCCC AAGATGGACC TGCTGACGGT GCCCGTGGTC 
GACACGGAGA TGGAGGCACG GCCCATGACA CTGGAGGAGA TGGAAGATGT GGGCAAGCAC 
TACCGAGAGA GGAAGCGGCA GAACAAGCTG GCCCTCCCCT CCATCCAGTA CACGGAGCAG 
TGCCGCCTGG TGCGCAGCGG GGACAAGCAC TTTGACGAGC ACTGCCTGCC GACCACAGTC 
CACGGGGAGA TGGGGAGCAT CCTCAACAGG TCCCGCGTGG ACACCTTCCT GGTCTACCTG 
CAGTGCTGGA AGGTCTGCGA GGCCCACGGC CTCCCGCTGA CCGAGGACAT CCTCATGAGA 
GCCCTGATGT ACCCAGGGGA CAAGATCATT TTCCAGAAGG ATGAGATGCG CCCGATCCGG 
CAGCCAGGAG GCTACTACTC AGACTTCAAG CCCTTCACTG AGAACCTGGC CCTGGTCCGG 
CCCCAGGGTG TTCCTGTGCC TGTGCCTCAG AAGAAGCCTG ACAAGAAAAT GCCGAAGAAA 
ATCAAGAAAA TCAATTTTAA GGAATTTGAG GAATTCACCA GGAAGCTGAA GGAGAAGAAG 
CCCAGCAGTC CACAGCTAAC CCATCCCAAC TTCTTCTGGC CGGGTCACCT CCTGGACAAG 
CTGCGGCTCT ACCTGCCCAC TGTGACCATG GACCGGAGCC TGGCCATCTT CAGCTGTGTC 
CAACAGCGGC CCCACGTCTA CCCGGCCACC TACCACTCCG ACTGCTGGTG GCCCATTAAG 
GACGTGAATT ACGTGACCTA CGCCTATTAC GATGCCCACA AGATCTATCA CATCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_024982591.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838359.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa228841
Clone ID Related Accession (Same CDS sequence)	XM_024982591.1
Accession Version	XM_024982591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGACACCGT CCCACGGACA 
CAAATAGAAA AAAGGAAAAC AAGCCTGTAT TTTTCCAGCA AATACAACAA AGAAGCTCTT 
AGCCCCCCAC GACGCAAGGC CTTTAAGAAG TGGACACCTC CGCGGTCACC TTTTAATCTC 
GTCCAGGAAA CTCTTTTTCA TGATCCGTGG AAGCTCCTCA TTGCAACTAT ATTTCTCAAT 
AGGACCTCAG GCAAAATGGC CATCCCTGTG CTCTGGCAGT TTCTGGAGAA GTACCCTTCT 
GCTGAGGCTG CAAGGACCGC AGACTGGAGA GATGTTTCAG AATTTCTTAA ACCTCTTGGT 
CTCTACGATC TTCGAGCAAA AACCATTATC AAGTTCTCCG ATGAATATCT GACAAAGCAG 
TGGAAGTATC CGATCGAGCT TCACGGGATT GGCAAATATG GCAACGACTC GTACCGAATT 
TTTTGCGTCA ATGAGTGGAA GCAGGTACAC CCTGAAGACC ATAAGTTAAA TAAATATCAT 
GACTGGCTTT GGGAAAATCA CGAGAAATTA AGTCTATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838359.1
CDS	206..1867
Translation	MESPSLRDCGAAPSVVDRERLVPDRLCDLREEDVALGSERVGEDEKQMVMKSSSECNLPLQEPFASAQSGGNAMTECRKSVPCGWERVVKQRLSGKTAGKYDVHFISPQGQKFRSRVSLVNYLHQSGETSLKPEDFDFSVCPERSIKSECKGRNTAALTFQLQHQSNSSNRNLRTRSMRKKDVFPLPSACLELQDSGELPNLKEDEGVHDIASRKVSKSKRKVTVLKEIQTKKTKPGCRKSLPDPVQSNRESQSEPVAQESHLEGTCCISDARASEQALSVTTEEERLGKKKSSCLGPNGEQIPSGIVSKLCSAKEVEPNNKCEDPLLESEEIRTKVEAAERKEHLHMDISEGGSETDDSSQTQKNPTSAKVSQDTVPRTQIEKRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWQFLEKYPSAEAARTADWRDVSEFLKPLGLYDLRAKTIIKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS

Target ORF information:

RefSeq Version	XM_024982591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982591.1

ORF Insert Sequence:

ATGGAGAGTC CGAGCCTGAG GGACTGCGGA GCTGCCCCCT CGGTCGTCGA CCGTGAGCGC 
CTAGTCCCAG ACCGGCTGTG TGACCTCCGG GAGGAAGATG TTGCTCTGGG GTCAGAAAGA 
GTGGGAGAGG ATGAAAAGCA AATGGTGATG AAAAGCAGCA GTGAATGTAA TCTCCCTCTA 
CAAGAGCCTT TTGCTTCTGC TCAATCTGGT GGGAACGCAA TGACAGAGTG CCGAAAGTCT 
GTACCGTGTG GATGGGAAAG AGTTGTGAAG CAAAGGTTAT CTGGGAAAAC AGCAGGCAAA 
TACGACGTAC ATTTTATCAG CCCACAGGGA CAGAAGTTCA GATCCAGAGT TTCACTTGTT 
AATTATCTTC ACCAAAGTGG AGAGACTTCT CTTAAGCCAG AAGACTTTGA TTTCAGTGTA 
TGTCCTGAAA GAAGCATCAA GTCAGAATGT AAAGGCCGAA ACACAGCAGC TCTGACATTC 
CAACTGCAAC ACCAAAGTAA CAGTTCAAAC CGGAACCTCA GGACACGGAG CATGAGGAAG 
AAAGATGTAT TTCCCCTGCC AAGTGCTTGT TTGGAATTGC AGGATAGCGG AGAACTACCC 
AACTTGAAAG AAGATGAGGG TGTTCATGAT ATTGCCTCCA GAAAGGTTAG CAAGTCCAAA 
AGAAAGGTGA CTGTTTTGAA AGAGATTCAA ACTAAGAAAA CAAAACCAGG GTGCCGCAAG 
AGTCTTCCTG ATCCTGTCCA AAGTAATAGA GAATCTCAAA GTGAACCTGT TGCACAGGAA 
AGTCACCTTG AGGGAACTTG CTGCATCTCT GATGCCAGAG CCAGCGAACA GGCCCTCAGT 
GTGACCACTG AAGAGGAGAG GCTTGGTAAA AAAAAATCAT CGTGTTTGGG ACCAAACGGT 
GAACAGATCC CTTCTGGCAT TGTAAGCAAA TTATGTTCGG CCAAAGAAGT GGAACCCAAC 
AATAAGTGTG AGGACCCCCT TTTAGAATCT GAGGAAATAA GAACCAAAGT AGAAGCTGCG 
GAGAGGAAGG AGCATTTGCA TATGGACATT TCAGAAGGTG GCTCTGAAAC AGACGACAGC 
TCACAAACAC AGAAAAACCC TACGTCTGCG AAAGTATCTC AAGACACCGT CCCACGGACA 
CAAATAGAAA AAAGGAAAAC AAGCCTGTAT TTTTCCAGCA AATACAACAA AGAAGCTCTT 
AGCCCCCCAC GACGCAAGGC CTTTAAGAAG TGGACACCTC CGCGGTCACC TTTTAATCTC 
GTCCAGGAAA CTCTTTTTCA TGATCCGTGG AAGCTCCTCA TTGCAACTAT ATTTCTCAAT 
AGGACCTCAG GCAAAATGGC CATCCCTGTG CTCTGGCAGT TTCTGGAGAA GTACCCTTCT 
GCTGAGGCTG CAAGGACCGC AGACTGGAGA GATGTTTCAG AATTTCTTAA ACCTCTTGGT 
CTCTACGATC TTCGAGCAAA AACCATTATC AAGTTCTCCG ATGAATATCT GACAAAGCAG 
TGGAAGTATC CGATCGAGCT TCACGGGATT GGCAAATATG GCAACGACTC GTACCGAATT 
TTTTGCGTCA ATGAGTGGAA GCAGGTACAC CCTGAAGACC ATAAGTTAAA TAAATATCAT 
GACTGGCTTT GGGAAAATCA CGAGAAATTA AGTCTATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD4 ( XM_024982590.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838358.1 Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X5, mRNA.

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CloneID	OBa31779
Clone ID Related Accession (Same CDS sequence)	XM_024982590.1
Accession Version	XM_024982590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA ACATCAGTCT TCAAATTTTG CACAATAGCC TACTGTCCTT GATCAGCACC 
ACAACTTTAG AGGCTGGGTA CCGGGGATAC ACAGGAAGTA AGACACAGTT CTTACCGTTG 
GGAGACTCAC AGGCTGTTGG GAAAAAGTAT CGGCATCAAG AAAAATATGG GTTCTACCAG 
TCTAAGTCCC TGTTGGATGA TGAAAATGCC AGCAAGGAGC CGGTGTTCAA CCCCGAAGCG 
GTCATCGCCC ACTGCTTCAA ACAGTTCAAG CAGGAGGACT TCCACCTGCC CAGGAGCCGC 
CGGCGGGTCA TCATCCTGCC ACAGAAGGAG GCTCCGAAGC CTGTCCGTCC CACGCCCCAA 
CTCCAGACTC CCCTGGAGCC CACCCCCAGC CCCAAAGCCC CGGAAGTCGG GGACACCCAA 
GAACAGCCAG AGGATCCGAG GGCGTGGCTC AGACAAAGGC TGAAGATGCG GAAGGACCTG 
GAATCGTTTG GCAACGTCGA GAGGTGGCTG CAGAACAAGG CCAACCTCAC GCTGTCCAAG 
GCCAAGGTCC TACGTGACAT CCACAAGAAG CAGCCCGTGG CTCGGCTGCC GAGCCAGCTG 
GCCATGATCA GGGACACTGC GAAGAAAAGC CAGCAACCCT CCCGCCGGCA GGTGCCCCAG 
CTCCGACTGC CCAAACCCCC CGCCCTGTCA ACCTTGTACT CCTACCTGCA CTGCCGCAAG 
ATCAAGATCC TGGAGCTGTT TCACAAGGTG GACCAGGGCA AGCACCAGAT CTCCCGAGAG 
GAGTTCATCG TGGCTCTCAA GGCGATTGGA GTCCCTCTGA AAAACCAGGA GGTGGAGGAC 
ATTGTGGTCT ACCTCAGCTC CCTGGGGAAG CACAACAGCA TCACGATGGA TAACCTGGCC 
AGCACCTACA AGCAGTGGTC TTTGGCTCGG CAGCAAAGCA CCCTGACCAT CGCAACAGAG 
GATTCCAGAT CATCCAATGA CAGCGGCTCC ACACAGAGTT CATCCCCAAA GCAGAAGGCG 
AATGTACCCC CAGAGGCTCC CAAGATGGAC CTGCTGACGG TGCCCGTGGT CGACACGGAG 
ATGGAGGCAC GGCCCATGAC ACTGGAGGAG ATGGAAGATG TGGGCAAGCA CTACCGAGAG 
AGGAAGCGGC AGAACAAGCT GGCCCTCCCC TCCATCCAGT ACACGGAGCA GTGCCGCCTG 
GTGCGCAGCG GGGACAAGCA CTTTGACGAG CACTGCCTGC CGACCACAGT CCACGGGGAG 
ATGGGGAGCA TCCTCAACAG GTCCCGCGTG GACACCTTCC TGGTCTACCT GCAGTGCTGG 
AAGGTCTGCG AGGCCCACGG CCTCCCGCTG ACCGAGGACA TCCTCATGAG AGCCCTGATG 
TACCCAGGGG ACAAGATCAT TTTCCAGAAG GATGAGATGC GCCCGATCCG GCAGCCAGGA 
GGCTACTACT CAGACTTCAA GCCCTTCACT GAGAACCTGG CCCTGGTCCG GCCCCAGGGT 
GTTCCTGTGC CTGTGCCTCA GAAGAAGCCT GACAAGAAAA TGCCGAAGAA AATCAAGAAA 
ATCAATTTTA AGGAATTTGA GGAATTCACC AGGAAGCTGA AGGAGAAGAA GCCCAGCAGT 
CCACAGCTAA CCCATCCCAA CTTCTTCTGG CCGGGTCACC TCCTGGACAA GCTGCGGCTC 
TACCTGCCCA CTGTGACCAT GGACCGGAGC CTGGCCATCT TCAGCTGTGT CCAACAGCGG 
CCCCACGTCT ACCCGGCCAC CTACCACTCC GACTGCTGGT GGCCCATTAA GGACGTGAAT 
TACGTGACCT ACGCCTATTA CGATGCCCAC AAGATCTATC ACATCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838358.1
CDS	1181..3031
Translation	MEKNISLQILHNSLLSLISTTTLEAGYRGYTGSKTQFLPLGDSQAVGKKYRHQEKYGFYQSKSLLDDENASKEPVFNPEAVIAHCFKQFKQEDFHLPRSRRRVIILPQKEAPKPVRPTPQLQTPLEPTPSPKAPEVGDTQEQPEDPRAWLRQRLKMRKDLESFGNVERWLQNKANLTLSKAKVLRDIHKKQPVARLPSQLAMIRDTAKKSQQPSRRQVPQLRLPKPPALSTLYSYLHCRKIKILELFHKVDQGKHQISREEFIVALKAIGVPLKNQEVEDIVVYLSSLGKHNSITMDNLASTYKQWSLARQQSTLTIATEDSRSSNDSGSTQSSSPKQKANVPPEAPKMDLLTVPVVDTEMEARPMTLEEMEDVGKHYRERKRQNKLALPSIQYTEQCRLVRSGDKHFDEHCLPTTVHGEMGSILNRSRVDTFLVYLQCWKVCEAHGLPLTEDILMRALMYPGDKIIFQKDEMRPIRQPGGYYSDFKPFTENLALVRPQGVPVPVPQKKPDKKMPKKIKKINFKEFEEFTRKLKEKKPSSPQLTHPNFFWPGHLLDKLRLYLPTVTMDRSLAIFSCVQQRPHVYPATYHSDCWWPIKDVNYVTYAYYDAHKIYHIN

Target ORF information:

RefSeq Version	XM_024982590.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain 4, DNA glycosylase (MBD4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982590.1

ORF Insert Sequence:

ATGGAAAAGA ACATCAGTCT TCAAATTTTG CACAATAGCC TACTGTCCTT GATCAGCACC 
ACAACTTTAG AGGCTGGGTA CCGGGGATAC ACAGGAAGTA AGACACAGTT CTTACCGTTG 
GGAGACTCAC AGGCTGTTGG GAAAAAGTAT CGGCATCAAG AAAAATATGG GTTCTACCAG 
TCTAAGTCCC TGTTGGATGA TGAAAATGCC AGCAAGGAGC CGGTGTTCAA CCCCGAAGCG 
GTCATCGCCC ACTGCTTCAA ACAGTTCAAG CAGGAGGACT TCCACCTGCC CAGGAGCCGC 
CGGCGGGTCA TCATCCTGCC ACAGAAGGAG GCTCCGAAGC CTGTCCGTCC CACGCCCCAA 
CTCCAGACTC CCCTGGAGCC CACCCCCAGC CCCAAAGCCC CGGAAGTCGG GGACACCCAA 
GAACAGCCAG AGGATCCGAG GGCGTGGCTC AGACAAAGGC TGAAGATGCG GAAGGACCTG 
GAATCGTTTG GCAACGTCGA GAGGTGGCTG CAGAACAAGG CCAACCTCAC GCTGTCCAAG 
GCCAAGGTCC TACGTGACAT CCACAAGAAG CAGCCCGTGG CTCGGCTGCC GAGCCAGCTG 
GCCATGATCA GGGACACTGC GAAGAAAAGC CAGCAACCCT CCCGCCGGCA GGTGCCCCAG 
CTCCGACTGC CCAAACCCCC CGCCCTGTCA ACCTTGTACT CCTACCTGCA CTGCCGCAAG 
ATCAAGATCC TGGAGCTGTT TCACAAGGTG GACCAGGGCA AGCACCAGAT CTCCCGAGAG 
GAGTTCATCG TGGCTCTCAA GGCGATTGGA GTCCCTCTGA AAAACCAGGA GGTGGAGGAC 
ATTGTGGTCT ACCTCAGCTC CCTGGGGAAG CACAACAGCA TCACGATGGA TAACCTGGCC 
AGCACCTACA AGCAGTGGTC TTTGGCTCGG CAGCAAAGCA CCCTGACCAT CGCAACAGAG 
GATTCCAGAT CATCCAATGA CAGCGGCTCC ACACAGAGTT CATCCCCAAA GCAGAAGGCG 
AATGTACCCC CAGAGGCTCC CAAGATGGAC CTGCTGACGG TGCCCGTGGT CGACACGGAG 
ATGGAGGCAC GGCCCATGAC ACTGGAGGAG ATGGAAGATG TGGGCAAGCA CTACCGAGAG 
AGGAAGCGGC AGAACAAGCT GGCCCTCCCC TCCATCCAGT ACACGGAGCA GTGCCGCCTG 
GTGCGCAGCG GGGACAAGCA CTTTGACGAG CACTGCCTGC CGACCACAGT CCACGGGGAG 
ATGGGGAGCA TCCTCAACAG GTCCCGCGTG GACACCTTCC TGGTCTACCT GCAGTGCTGG 
AAGGTCTGCG AGGCCCACGG CCTCCCGCTG ACCGAGGACA TCCTCATGAG AGCCCTGATG 
TACCCAGGGG ACAAGATCAT TTTCCAGAAG GATGAGATGC GCCCGATCCG GCAGCCAGGA 
GGCTACTACT CAGACTTCAA GCCCTTCACT GAGAACCTGG CCCTGGTCCG GCCCCAGGGT 
GTTCCTGTGC CTGTGCCTCA GAAGAAGCCT GACAAGAAAA TGCCGAAGAA AATCAAGAAA 
ATCAATTTTA AGGAATTTGA GGAATTCACC AGGAAGCTGA AGGAGAAGAA GCCCAGCAGT 
CCACAGCTAA CCCATCCCAA CTTCTTCTGG CCGGGTCACC TCCTGGACAA GCTGCGGCTC 
TACCTGCCCA CTGTGACCAT GGACCGGAGC CTGGCCATCT TCAGCTGTGT CCAACAGCGG 
CCCCACGTCT ACCCGGCCAC CTACCACTCC GACTGCTGGT GGCCCATTAA GGACGTGAAT 
TACGTGACCT ACGCCTATTA CGATGCCCAC AAGATCTATC ACATCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MBD4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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