NEK4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK4
Entrez Gene ID	511455
Full Name	NIMA related kinase 4
General protein information	Preferred Names NIMA related kinase 4 Names serine/threonine-protein kinase Nek4 NIMA (never in mitosis gene a)-related kinase 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015459555.1	XP_015315041.1	serine/threonine-protein kinase Nek4 isoform X3
XM_024982583.1	XP_024838351.1	serine/threonine-protein kinase Nek4 isoform X1
XM_005222775.3	XP_005222832.1	serine/threonine-protein kinase Nek4 isoform X2
XM_024982585.1	XP_024838353.1	serine/threonine-protein kinase Nek4 isoform X3
XM_024982586.1	XP_024838354.1	serine/threonine-protein kinase Nek4 isoform X4
XM_005222774.4	XP_005222831.1	serine/threonine-protein kinase Nek4 isoform X2
NM_001101944.2	NP_001095414.1	serine/threonine-protein kinase Nek4
XM_005222774.3	XP_005222831.1	serine/threonine-protein kinase Nek4 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NEK4	XP_414252.2
Danio rerio (zebrafish)	nek4	NP_957306.1
Xenopus tropicalis (tropical clawed frog)	nek4	NP_001072768.1
Homo sapiens (human)	NEK4	NP_003148.2
Pan troglodytes (chimpanzee)	NEK4	XP_516519.4
Canis lupus familiaris (dog)	NEK4	XP_005632492.1
Bos taurus (cattle)	NEK4	NP_001095414.1
Mus musculus (house mouse)	Nek4	NP_035979.1
Rattus norvegicus (Norway rat)	Nek4	NP_001013152.2
	NEK4	XP_002802834.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37057	NM_001101944.2 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa31540	XM_005222774.4 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa31541	XM_005222775.3 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa228834	XM_024982583.1 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa228835	XM_024982585.1 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa228836	XM_024982586.1 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa94412	XM_015459555.1 Latest version!	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa31540	XM_005222774.3	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK4 ( NM_001101944.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095414.1 Bos taurus NIMA related kinase 4 (NEK4), mRNA.

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CloneID	OBa37057
Clone ID Related Accession (Same CDS sequence)	NM_001101944.2
Accession Version	NM_001101944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149593.1. On Aug 31, 2012 this sequence version replaced NM_001101944.1. ##Evidence-Data-START## Transcript exon combination :: BC149593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTTCT GTGAAGGAGG TGATCTGTAC CGAAAGCTCA AAGAGCAGAA AGGGAGGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTTCAGATCG CCATGGCTCT GCAGTATTTA 
CATGAAAAAC ACATCCTCCA CCGAGATCTA AAAACCCAAA ATGTCTTCCT GACAAGAACA 
AATATCATCA AAGTGGGTGA CCTTGGAATT GCCCGCGTGC TTGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCGTACTAT ATGAGCCCTG AACTGTTTTC GAACAAACCC 
TACAACTACA AGTCTGATGT TTGGGCTCTG GGATGCTGTG TTTATGAAAT GGCCACCCTG 
AAGCATGCTT TCAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAAAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACTATGCTG 
AGCAAGAGAC CTGAAGAAAG ACCTTCTGTG AGGAGCATCT TGAGGCAGCC TTACATAAAG 
CGCCAAATAT CCTTGTTTTT GGAGGCCACG AAGGCAAAAA CCTCCAAAAA TAATGTTAAA 
AATGGTGACT CTAAATCCAA GCCTGTGGCT GCAGTGGTTC CTGGAAAGGC TGAATTAAGT 
CATGAAGTAG TTCCCCTTCA ATCACACTCT TCTGAGAGCT CCAAGACATA TGTAATGGGT 
GAAGACAAAT GTTTGTTCCA GGAGAAACCT GTAGTCATTG GCCCCTTGAA GATACCCACA 
AGTCTGAAAG ACCACATCTA CAAACCAGAC ATAAGCAATA CTGTGGACTC ACTAGCCACA 
ATCAGTAGGG TGAATATTGA CATCTTACCT ACAGAGAGGA GGAACTCAAT GAATGACGGC 
TTAGTTCAAG AGAACCCACC AGGGCACCTA GATGCCTCTA ATGAACTGGA AGGTAAATGT 
AATGTTTCTC AAGTGACGGA GATGTTGCAG AACAGTAAAT CCAGTGCTCT ACCTGGAAAC 
CGAATTCCCA CATTGTCCTC TCACTGTGTC ACTGGAGAAA GGAATGACCC AATGAAGCCT 
CTGCAGCCCC TAAACAAAGA CCAAAATTTA CCAAAAGACC AGGATCAAGT TGGTGGTGAA 
TGTGTTACAG GAAAACAGGA CAGAATCCAC CCAGCTTTAC AGCCACACAG CTCTGGGTCT 
GAACCTTCCC AGTCTCGACA GCGGCGGCAG AAGAAAAGAG AACACGCTGA GCACAGTGGA 
GAAAAGAGAC AGTTCCAAGA GACTCCGCCT TGCCTTTTGC CTTCTCTTCC CACTGTTGGA 
AAAATGGAAT GTACATCAAC ACAAAAAGAT GCTGAAAACC AAAGTAGAGT GGTCACTGGA 
TCTGTGAACA GTTCGAGGGG CAGTGAGATA GCATCATCAA AGGACCGACC ATTATCAGCA 
AGAGAGAGGA GGCGACTGAA ACAGTCACAG GAAGAGATCT TCCCCTCAGG CCCTGCAGTG 
AGGAGAGCTT CTCTGGGTCC AGCGGGACCA GCGAAACCAC AGAAAGACCA CCTCAGCCCT 
GCTCGAAGGT CATCTTCCGA CTGCAGTGTT GCTCAGGAAA GGAAGCTCAC CCATTGTCTC 
TCAGAGGATG AGTTAAGTTC CTCTACCAGT TCAACTGATA AATCAGATGG GGATTCCAGG 
GAAGGTAAAG GTCATACAAA TGAAATGACT GACTTGGTAC AATTGATGAC TCAGACTCTG 
AAGTTGGACT CTAAAGAGAG CTATGAAGAT CTCCCAGTAC CAGATCCAGT GTCAGAATTT 
AAACTTCATC GGAAGTATCG GGACACGCTA ATACTTCATG GGAAAGTTGC AGAAGAGGCA 
GAGGAACTCC ATTTTAAAGA GCTACCTTCA GCGATCATGC CAGGTTCTGA AAAAATCAGA 
AGAATAGTTG AAGTCTTAAG AGCTGATGTA ATTCGGGGCC TGGGGATTCA GCTTTTAGAG 
CAGGTGTATG ATATTTTGGA AGAGGAGGAT GAATTGGAAA GAGAGGTACA TTTGCAGGAG 
CACATGGGTG AAAAGTATGT GACTTACAGT GTGAAAGCTC GCCAGTTAAA ATTTTTTGAA 
GAAAATGTGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095414.1
CDS	411..2528
Translation	MGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	NM_001101944.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101944.2

ORF Insert Sequence:

ATGGGCTTCT GTGAAGGAGG TGATCTGTAC CGAAAGCTCA AAGAGCAGAA AGGGAGGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTTCAGATCG CCATGGCTCT GCAGTATTTA 
CATGAAAAAC ACATCCTCCA CCGAGATCTA AAAACCCAAA ATGTCTTCCT GACAAGAACA 
AATATCATCA AAGTGGGTGA CCTTGGAATT GCCCGCGTGC TTGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCGTACTAT ATGAGCCCTG AACTGTTTTC GAACAAACCC 
TACAACTACA AGTCTGATGT TTGGGCTCTG GGATGCTGTG TTTATGAAAT GGCCACCCTG 
AAGCATGCTT TCAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAAAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACTATGCTG 
AGCAAGAGAC CTGAAGAAAG ACCTTCTGTG AGGAGCATCT TGAGGCAGCC TTACATAAAG 
CGCCAAATAT CCTTGTTTTT GGAGGCCACG AAGGCAAAAA CCTCCAAAAA TAATGTTAAA 
AATGGTGACT CTAAATCCAA GCCTGTGGCT GCAGTGGTTC CTGGAAAGGC TGAATTAAGT 
CATGAAGTAG TTCCCCTTCA ATCACACTCT TCTGAGAGCT CCAAGACATA TGTAATGGGT 
GAAGACAAAT GTTTGTTCCA GGAGAAACCT GTAGTCATTG GCCCCTTGAA GATACCCACA 
AGTCTGAAAG ACCACATCTA CAAACCAGAC ATAAGCAATA CTGTGGACTC ACTAGCCACA 
ATCAGTAGGG TGAATATTGA CATCTTACCT ACAGAGAGGA GGAACTCAAT GAATGACGGC 
TTAGTTCAAG AGAACCCACC AGGGCACCTA GATGCCTCTA ATGAACTGGA AGGTAAATGT 
AATGTTTCTC AAGTGACGGA GATGTTGCAG AACAGTAAAT CCAGTGCTCT ACCTGGAAAC 
CGAATTCCCA CATTGTCCTC TCACTGTGTC ACTGGAGAAA GGAATGACCC AATGAAGCCT 
CTGCAGCCCC TAAACAAAGA CCAAAATTTA CCAAAAGACC AGGATCAAGT TGGTGGTGAA 
TGTGTTACAG GAAAACAGGA CAGAATCCAC CCAGCTTTAC AGCCACACAG CTCTGGGTCT 
GAACCTTCCC AGTCTCGACA GCGGCGGCAG AAGAAAAGAG AACACGCTGA GCACAGTGGA 
GAAAAGAGAC AGTTCCAAGA GACTCCGCCT TGCCTTTTGC CTTCTCTTCC CACTGTTGGA 
AAAATGGAAT GTACATCAAC ACAAAAAGAT GCTGAAAACC AAAGTAGAGT GGTCACTGGA 
TCTGTGAACA GTTCGAGGGG CAGTGAGATA GCATCATCAA AGGACCGACC ATTATCAGCA 
AGAGAGAGGA GGCGACTGAA ACAGTCACAG GAAGAGATCT TCCCCTCAGG CCCTGCAGTG 
AGGAGAGCTT CTCTGGGTCC AGCGGGACCA GCGAAACCAC AGAAAGACCA CCTCAGCCCT 
GCTCGAAGGT CATCTTCCGA CTGCAGTGTT GCTCAGGAAA GGAAGCTCAC CCATTGTCTC 
TCAGAGGATG AGTTAAGTTC CTCTACCAGT TCAACTGATA AATCAGATGG GGATTCCAGG 
GAAGGTAAAG GTCATACAAA TGAAATGACT GACTTGGTAC AATTGATGAC TCAGACTCTG 
AAGTTGGACT CTAAAGAGAG CTATGAAGAT CTCCCAGTAC CAGATCCAGT GTCAGAATTT 
AAACTTCATC GGAAGTATCG GGACACGCTA ATACTTCATG GGAAAGTTGC AGAAGAGGCA 
GAGGAACTCC ATTTTAAAGA GCTACCTTCA GCGATCATGC CAGGTTCTGA AAAAATCAGA 
AGAATAGTTG AAGTCTTAAG AGCTGATGTA ATTCGGGGCC TGGGGATTCA GCTTTTAGAG 
CAGGTGTATG ATATTTTGGA AGAGGAGGAT GAATTGGAAA GAGAGGTACA TTTGCAGGAG 
CACATGGGTG AAAAGTATGT GACTTACAGT GTGAAAGCTC GCCAGTTAAA ATTTTTTGAA 
GAAAATGTGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_005222774.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222831.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa31540
Clone ID Related Accession (Same CDS sequence)	XM_005222774.4 , XM_005222774.3
Accession Version	XM_005222774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222774.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GGTACATTTG 
CAGGAGCACA TGGGTGAAAA GTATGTGACT TACAGTGTGA AAGCTCGCCA GTTAAAATTT 
TTTGAAGAAA ATGTGAATTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222831.1
CDS	230..2593
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	XM_005222774.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222774.4

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GGTACATTTG 
CAGGAGCACA TGGGTGAAAA GTATGTGACT TACAGTGTGA AAGCTCGCCA GTTAAAATTT 
TTTGAAGAAA ATGTGAATTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_005222774.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222831.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31540
Clone ID Related Accession (Same CDS sequence)	XM_005222774.4 , XM_005222774.3
Accession Version	XM_005222774.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GGTACATTTG 
CAGGAGCACA TGGGTGAAAA GTATGTGACT TACAGTGTGA AAGCTCGCCA GTTAAAATTT 
TTTGAAGAAA ATGTGAATTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222831.1
CDS	231..2594
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	XM_005222774.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222774.3

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GGTACATTTG 
CAGGAGCACA TGGGTGAAAA GTATGTGACT TACAGTGTGA AAGCTCGCCA GTTAAAATTT 
TTTGAAGAAA ATGTGAATTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_005222775.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222832.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa31541
Clone ID Related Accession (Same CDS sequence)	XM_005222775.3
Accession Version	XM_005222775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GTCTCCCTGT 
CTGGCCCTAG CACCAGGGCA ATGCTGGCCT CATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222832.1
CDS	231..2549
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELERESPCLALAPGQCWPHE

Target ORF information:

RefSeq Version	XM_005222775.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222775.3

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGCGA TCATGCCAGG TTCTGAAAAA 
ATCAGAAGAA TAGTTGAAGT CTTAAGAGCT GATGTAATTC GGGGCCTGGG GATTCAGCTT 
TTAGAGCAGG TGTATGATAT TTTGGAAGAG GAGGATGAAT TGGAAAGAGA GTCTCCCTGT 
CTGGCCCTAG CACCAGGGCA ATGCTGGCCT CATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_024982583.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838351.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228834
Clone ID Related Accession (Same CDS sequence)	XM_024982583.1
Accession Version	XM_024982583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGGTG CGATCATGCC AGGTTCTGAA 
AAAATCAGAA GAATAGTTGA AGTCTTAAGA GCTGATGTAA TTCGGGGCCT GGGGATTCAG 
CTTTTAGAGC AGGTGTATGA TATTTTGGAA GAGGAGGATG AATTGGAAAG AGAGGTACAT 
TTGCAGGAGC ACATGGGTGA AAAGTATGTG ACTTACAGTG TGAAAGCTCG CCAGTTAAAA 
TTTTTTGAAG AAAATGTGAA TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838351.1
CDS	230..2596
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSGAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	XM_024982583.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982583.1

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGGTG CGATCATGCC AGGTTCTGAA 
AAAATCAGAA GAATAGTTGA AGTCTTAAGA GCTGATGTAA TTCGGGGCCT GGGGATTCAG 
CTTTTAGAGC AGGTGTATGA TATTTTGGAA GAGGAGGATG AATTGGAAAG AGAGGTACAT 
TTGCAGGAGC ACATGGGTGA AAAGTATGTG ACTTACAGTG TGAAAGCTCG CCAGTTAAAA 
TTTTTTGAAG AAAATGTGAA TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_024982585.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838353.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228835
Clone ID Related Accession (Same CDS sequence)	XM_024982585.1
Accession Version	XM_024982585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGGTG CGATCATGCC AGGTTCTGAA 
AAAATCAGAA GAATAGTTGA AGTCTTAAGA GCTGATGTAA TTCGGGGCCT GGGGATTCAG 
CTTTTAGAGC AGGTGTATGA TATTTTGGAA GAGGAGGATG AATTGGAAAG AGAGTCTCCC 
TGTCTGGCCC TAGCACCAGG GCAATGCTGG CCTCATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838353.1
CDS	230..2551
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSGAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELERESPCLALAPGQCWPHE

Target ORF information:

RefSeq Version	XM_024982585.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982585.1

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGTC TGATGTTTGG GCTCTGGGAT GCTGTGTTTA TGAAATGGCC 
ACCCTGAAGC ATGCTTTCAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAAAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACT 
ATGCTGAGCA AGAGACCTGA AGAAAGACCT TCTGTGAGGA GCATCTTGAG GCAGCCTTAC 
ATAAAGCGCC AAATATCCTT GTTTTTGGAG GCCACGAAGG CAAAAACCTC CAAAAATAAT 
GTTAAAAATG GTGACTCTAA ATCCAAGCCT GTGGCTGCAG TGGTTCCTGG AAAGGCTGAA 
TTAAGTCATG AAGTAGTTCC CCTTCAATCA CACTCTTCTG AGAGCTCCAA GACATATGTA 
ATGGGTGAAG ACAAATGTTT GTTCCAGGAG AAACCTGTAG TCATTGGCCC CTTGAAGATA 
CCCACAAGTC TGAAAGACCA CATCTACAAA CCAGACATAA GCAATACTGT GGACTCACTA 
GCCACAATCA GTAGGGTGAA TATTGACATC TTACCTACAG AGAGGAGGAA CTCAATGAAT 
GACGGCTTAG TTCAAGAGAA CCCACCAGGG CACCTAGATG CCTCTAATGA ACTGGAAGGT 
AAATGTAATG TTTCTCAAGT GACGGAGATG TTGCAGAACA GTAAATCCAG TGCTCTACCT 
GGAAACCGAA TTCCCACATT GTCCTCTCAC TGTGTCACTG GAGAAAGGAA TGACCCAATG 
AAGCCTCTGC AGCCCCTAAA CAAAGACCAA AATTTACCAA AAGACCAGGA TCAAGTTGGT 
GGTGAATGTG TTACAGGAAA ACAGGACAGA ATCCACCCAG CTTTACAGCC ACACAGCTCT 
GGGTCTGAAC CTTCCCAGTC TCGACAGCGG CGGCAGAAGA AAAGAGAACA CGCTGAGCAC 
AGTGGAGAAA AGAGACAGTT CCAAGAGACT CCGCCTTGCC TTTTGCCTTC TCTTCCCACT 
GTTGGAAAAA TGGAATGTAC ATCAACACAA AAAGATGCTG AAAACCAAAG TAGAGTGGTC 
ACTGGATCTG TGAACAGTTC GAGGGGCAGT GAGATAGCAT CATCAAAGGA CCGACCATTA 
TCAGCAAGAG AGAGGAGGCG ACTGAAACAG TCACAGGAAG AGATCTTCCC CTCAGGCCCT 
GCAGTGAGGA GAGCTTCTCT GGGTCCAGCG GGACCAGCGA AACCACAGAA AGACCACCTC 
AGCCCTGCTC GAAGGTCATC TTCCGACTGC AGTGTTGCTC AGGAAAGGAA GCTCACCCAT 
TGTCTCTCAG AGGATGAGTT AAGTTCCTCT ACCAGTTCAA CTGATAAATC AGATGGGGAT 
TCCAGGGAAG GTAAAGGTCA TACAAATGAA ATGACTGACT TGGTACAATT GATGACTCAG 
ACTCTGAAGT TGGACTCTAA AGAGAGCTAT GAAGATCTCC CAGTACCAGA TCCAGTGTCA 
GAATTTAAAC TTCATCGGAA GTATCGGGAC ACGCTAATAC TTCATGGGAA AGTTGCAGAA 
GAGGCAGAGG AACTCCATTT TAAAGAGCTA CCTTCAGGTG CGATCATGCC AGGTTCTGAA 
AAAATCAGAA GAATAGTTGA AGTCTTAAGA GCTGATGTAA TTCGGGGCCT GGGGATTCAG 
CTTTTAGAGC AGGTGTATGA TATTTTGGAA GAGGAGGATG AATTGGAAAG AGAGTCTCCC 
TGTCTGGCCC TAGCACCAGG GCAATGCTGG CCTCATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_024982586.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838354.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228836
Clone ID Related Accession (Same CDS sequence)	XM_024982586.1
Accession Version	XM_024982586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCT GTGAAGGAGG TGATCTGTAC CGAAAGCTCA AAGAGCAGAA AGGGAGGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTTCAGATCG CCATGGCTCT GCAGTATTTA 
CATGAAAAAC ACATCCTCCA CCGAGATCTA AAAACCCAAA ATGTCTTCCT GACAAGAACA 
AATATCATCA AAGTGGGTGA CCTTGGAATT GCCCGCGTGC TTGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCGTACTAT ATGAGCCCTG AACTGTTTTC GAACAAACCC 
TACAACTACA AGTCTGATGT TTGGGCTCTG GGATGCTGTG TTTATGAAAT GGCCACCCTG 
AAGCATGCTT TCAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAAAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACTATGCTG 
AGCAAGAGAC CTGAAGAAAG ACCTTCTGTG AGGAGCATCT TGAGGCAGCC TTACATAAAG 
CGCCAAATAT CCTTGTTTTT GGAGGCCACG AAGGCAAAAA CCTCCAAAAA TAATGTTAAA 
AATGGTGACT CTAAATCCAA GCCTGTGGCT GCAGTGGTTC CTGGAAAGGC TGAATTAAGT 
CATGAAGTAG TTCCCCTTCA ATCACACTCT TCTGAGAGCT CCAAGACATA TGTAATGGGT 
GAAGACAAAT GTTTGTTCCA GGAGAAACCT GTAGTCATTG GCCCCTTGAA GATACCCACA 
AGTCTGAAAG ACCACATCTA CAAACCAGAC ATAAGCAATA CTGTGGACTC ACTAGCCACA 
ATCAGTAGGG TGAATATTGA CATCTTACCT ACAGAGAGGA GGAACTCAAT GAATGACGGC 
TTAGTTCAAG AGAACCCACC AGGGCACCTA GATGCCTCTA ATGAACTGGA AGGTAAATGT 
AATGTTTCTC AAGTGACGGA GATGTTGCAG AACAGTAAAT CCAGTGCTCT ACCTGGAAAC 
CGAATTCCCA CATTGTCCTC TCACTGTGTC ACTGGAGAAA GGAATGACCC AATGAAGCCT 
CTGCAGCCCC TAAACAAAGA CCAAAATTTA CCAAAAGACC AGGATCAAGT TGGTGGTGAA 
TGTGTTACAG GAAAACAGGA CAGAATCCAC CCAGCTTTAC AGCCACACAG CTCTGGGTCT 
GAACCTTCCC AGTCTCGACA GCGGCGGCAG AAGAAAAGAG AACACGCTGA GCACAGTGGA 
GAAAAGAGAC AGTTCCAAGA GACTCCGCCT TGCCTTTTGC CTTCTCTTCC CACTGTTGGA 
AAAATGGAAT GTACATCAAC ACAAAAAGAT GCTGAAAACC AAAGTAGAGT GGTCACTGGA 
TCTGTGAACA GTTCGAGGGG CAGTGAGATA GCATCATCAA AGGACCGACC ATTATCAGCA 
AGAGAGAGGA GGCGACTGAA ACAGTCACAG GAAGAGATCT TCCCCTCAGG CCCTGCAGTG 
AGGAGAGCTT CTCTGGGTCC AGCGGGACCA GCGAAACCAC AGAAAGACCA CCTCAGCCCT 
GCTCGAAGGT CATCTTCCGA CTGCAGTGTT GCTCAGGAAA GGAAGCTCAC CCATTGTCTC 
TCAGAGGATG AGTTAAGTTC CTCTACCAGT TCAACTGATA AATCAGATGG GGATTCCAGG 
GAAGGTAAAG GTCATACAAA TGAAATGACT GACTTGGTAC AATTGATGAC TCAGACTCTG 
AAGTTGGACT CTAAAGAGAG CTATGAAGAT CTCCCAGTAC CAGATCCAGT GTCAGAATTT 
AAACTTCATC GGAAGTATCG GGACACGCTA ATACTTCATG GGAAAGTTGC AGAAGAGGCA 
GAGGAACTCC ATTTTAAAGA GCTACCTTCA GGTGCGATCA TGCCAGGTTC TGAAAAAATC 
AGAAGAATAG TTGAAGTCTT AAGAGCTGAT GTAATTCGGG GCCTGGGGAT TCAGCTTTTA 
GAGCAGGTGT ATGATATTTT GGAAGAGGAG GATGAATTGG AAAGAGAGGT ACATTTGCAG 
GAGCACATGG GTGAAAAGTA TGTGACTTAC AGTGTGAAAG CTCGCCAGTT AAAATTTTTT 
GAAGAAAATG TGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838354.1
CDS	431..2551
Translation	MGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSGAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	XM_024982586.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982586.1

ORF Insert Sequence:

ATGGGCTTCT GTGAAGGAGG TGATCTGTAC CGAAAGCTCA AAGAGCAGAA AGGGAGGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTTCAGATCG CCATGGCTCT GCAGTATTTA 
CATGAAAAAC ACATCCTCCA CCGAGATCTA AAAACCCAAA ATGTCTTCCT GACAAGAACA 
AATATCATCA AAGTGGGTGA CCTTGGAATT GCCCGCGTGC TTGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCGTACTAT ATGAGCCCTG AACTGTTTTC GAACAAACCC 
TACAACTACA AGTCTGATGT TTGGGCTCTG GGATGCTGTG TTTATGAAAT GGCCACCCTG 
AAGCATGCTT TCAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAAAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACTATGCTG 
AGCAAGAGAC CTGAAGAAAG ACCTTCTGTG AGGAGCATCT TGAGGCAGCC TTACATAAAG 
CGCCAAATAT CCTTGTTTTT GGAGGCCACG AAGGCAAAAA CCTCCAAAAA TAATGTTAAA 
AATGGTGACT CTAAATCCAA GCCTGTGGCT GCAGTGGTTC CTGGAAAGGC TGAATTAAGT 
CATGAAGTAG TTCCCCTTCA ATCACACTCT TCTGAGAGCT CCAAGACATA TGTAATGGGT 
GAAGACAAAT GTTTGTTCCA GGAGAAACCT GTAGTCATTG GCCCCTTGAA GATACCCACA 
AGTCTGAAAG ACCACATCTA CAAACCAGAC ATAAGCAATA CTGTGGACTC ACTAGCCACA 
ATCAGTAGGG TGAATATTGA CATCTTACCT ACAGAGAGGA GGAACTCAAT GAATGACGGC 
TTAGTTCAAG AGAACCCACC AGGGCACCTA GATGCCTCTA ATGAACTGGA AGGTAAATGT 
AATGTTTCTC AAGTGACGGA GATGTTGCAG AACAGTAAAT CCAGTGCTCT ACCTGGAAAC 
CGAATTCCCA CATTGTCCTC TCACTGTGTC ACTGGAGAAA GGAATGACCC AATGAAGCCT 
CTGCAGCCCC TAAACAAAGA CCAAAATTTA CCAAAAGACC AGGATCAAGT TGGTGGTGAA 
TGTGTTACAG GAAAACAGGA CAGAATCCAC CCAGCTTTAC AGCCACACAG CTCTGGGTCT 
GAACCTTCCC AGTCTCGACA GCGGCGGCAG AAGAAAAGAG AACACGCTGA GCACAGTGGA 
GAAAAGAGAC AGTTCCAAGA GACTCCGCCT TGCCTTTTGC CTTCTCTTCC CACTGTTGGA 
AAAATGGAAT GTACATCAAC ACAAAAAGAT GCTGAAAACC AAAGTAGAGT GGTCACTGGA 
TCTGTGAACA GTTCGAGGGG CAGTGAGATA GCATCATCAA AGGACCGACC ATTATCAGCA 
AGAGAGAGGA GGCGACTGAA ACAGTCACAG GAAGAGATCT TCCCCTCAGG CCCTGCAGTG 
AGGAGAGCTT CTCTGGGTCC AGCGGGACCA GCGAAACCAC AGAAAGACCA CCTCAGCCCT 
GCTCGAAGGT CATCTTCCGA CTGCAGTGTT GCTCAGGAAA GGAAGCTCAC CCATTGTCTC 
TCAGAGGATG AGTTAAGTTC CTCTACCAGT TCAACTGATA AATCAGATGG GGATTCCAGG 
GAAGGTAAAG GTCATACAAA TGAAATGACT GACTTGGTAC AATTGATGAC TCAGACTCTG 
AAGTTGGACT CTAAAGAGAG CTATGAAGAT CTCCCAGTAC CAGATCCAGT GTCAGAATTT 
AAACTTCATC GGAAGTATCG GGACACGCTA ATACTTCATG GGAAAGTTGC AGAAGAGGCA 
GAGGAACTCC ATTTTAAAGA GCTACCTTCA GGTGCGATCA TGCCAGGTTC TGAAAAAATC 
AGAAGAATAG TTGAAGTCTT AAGAGCTGAT GTAATTCGGG GCCTGGGGAT TCAGCTTTTA 
GAGCAGGTGT ATGATATTTT GGAAGAGGAG GATGAATTGG AAAGAGAGGT ACATTTGCAG 
GAGCACATGG GTGAAAAGTA TGTGACTTAC AGTGTGAAAG CTCGCCAGTT AAAATTTTTT 
GAAGAAAATG TGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_015459555.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315041.1 Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

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CloneID	OBa94412
Clone ID Related Accession (Same CDS sequence)	XM_015459555.1
Accession Version	XM_015459555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGCT GCCACCAATG CCAAAAGATT ACAGCCCAGA GCTGGCAGAA 
CTGATAAGAA CTATGCTGAG CAAGAGACCT GAAGAAAGAC CTTCTGTGAG GAGCATCTTG 
AGGCAGCCTT ACATAAAGCG CCAAATATCC TTGTTTTTGG AGGCCACGAA GGCAAAAACC 
TCCAAAAATA ATGTTAAAAA TGGTGACTCT AAATCCAAGC CTGTGGCTGC AGTGGTTCCT 
GGAAAGGCTG AATTAAGTCA TGAAGTAGTT CCCCTTCAAT CACACTCTTC TGAGAGCTCC 
AAGACATATG TAATGGGTGA AGACAAATGT TTGTTCCAGG AGAAACCTGT AGTCATTGGC 
CCCTTGAAGA TACCCACAAG TCTGAAAGAC CACATCTACA AACCAGACAT AAGCAATACT 
GTGGACTCAC TAGCCACAAT CAGTAGGGTG AATATTGACA TCTTACCTAC AGAGAGGAGG 
AACTCAATGA ATGACGGCTT AGTTCAAGAG AACCCACCAG GGCACCTAGA TGCCTCTAAT 
GAACTGGAAG GTAAATGTAA TGTTTCTCAA GTGACGGAGA TGTTGCAGAA CAGTAAATCC 
AGTGCTCTAC CTGGAAACCG AATTCCCACA TTGTCCTCTC ACTGTGTCAC TGGAGAAAGG 
AATGACCCAA TGAAGCCTCT GCAGCCCCTA AACAAAGACC AAAATTTACC AAAAGACCAG 
GATCAAGTTG GTGGTGAATG TGTTACAGGA AAACAGGACA GAATCCACCC AGCTTTACAG 
CCACACAGCT CTGGGTCTGA ACCTTCCCAG TCTCGACAGC GGCGGCAGAA GAAAAGAGAA 
CACGCTGAGC ACAGTGGAGA AAAGAGACAG TTCCAAGAGA CTCCGCCTTG CCTTTTGCCT 
TCTCTTCCCA CTGTTGGAAA AATGGAATGT ACATCAACAC AAAAAGATGC TGAAAACCAA 
AGTAGAGTGG TCACTGGATC TGTGAACAGT TCGAGGGGCA GTGAGATAGC ATCATCAAAG 
GACCGACCAT TATCAGCAAG AGAGAGGAGG CGACTGAAAC AGTCACAGGA AGAGATCTTC 
CCCTCAGGCC CTGCAGTGAG GAGAGCTTCT CTGGGTCCAG CGGGACCAGC GAAACCACAG 
AAAGACCACC TCAGCCCTGC TCGAAGGTCA TCTTCCGACT GCAGTGTTGC TCAGGAAAGG 
AAGCTCACCC ATTGTCTCTC AGAGGATGAG TTAAGTTCCT CTACCAGTTC AACTGATAAA 
TCAGATGGGG ATTCCAGGGA AGGTAAAGGT CATACAAATG AAATGACTGA CTTGGTACAA 
TTGATGACTC AGACTCTGAA GTTGGACTCT AAAGAGAGCT ATGAAGATCT CCCAGTACCA 
GATCCAGTGT CAGAATTTAA ACTTCATCGG AAGTATCGGG ACACGCTAAT ACTTCATGGG 
AAAGTTGCAG AAGAGGCAGA GGAACTCCAT TTTAAAGAGC TACCTTCAGC GATCATGCCA 
GGTTCTGAAA AAATCAGAAG AATAGTTGAA GTCTTAAGAG CTGATGTAAT TCGGGGCCTG 
GGGATTCAGC TTTTAGAGCA GGTGTATGAT ATTTTGGAAG AGGAGGATGA ATTGGAAAGA 
GAGGTACATT TGCAGGAGCA CATGGGTGAA AAGTATGTGA CTTACAGTGT GAAAGCTCGC 
CAGTTAAAAT TTTTTGAAGA AAATGTGAAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315041.1
CDS	193..2448
Translation	MPLTAYCYLRVVGKGSYGEVTLVRHRRDGRQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGRLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKLPPMPKDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISLFLEATKAKTSKNNVKNGDSKSKPVAAVVPGKAELSHEVVPLQSHSSESSKTYVMGEDKCLFQEKPVVIGPLKIPTSLKDHIYKPDISNTVDSLATISRVNIDILPTERRNSMNDGLVQENPPGHLDASNELEGKCNVSQVTEMLQNSKSSALPGNRIPTLSSHCVTGERNDPMKPLQPLNKDQNLPKDQDQVGGECVTGKQDRIHPALQPHSSGSEPSQSRQRRQKKREHAEHSGEKRQFQETPPCLLPSLPTVGKMECTSTQKDAENQSRVVTGSVNSSRGSEIASSKDRPLSARERRRLKQSQEEIFPSGPAVRRASLGPAGPAKPQKDHLSPARRSSSDCSVAQERKLTHCLSEDELSSSTSSTDKSDGDSREGKGHTNEMTDLVQLMTQTLKLDSKESYEDLPVPDPVSEFKLHRKYRDTLILHGKVAEEAEELHFKELPSAIMPGSEKIRRIVEVLRADVIRGLGIQLLEQVYDILEEEDELEREVHLQEHMGEKYVTYSVKARQLKFFEENVNF

Target ORF information:

RefSeq Version	XM_015459555.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459555.1

ORF Insert Sequence:

ATGCCCCTGA CCGCCTACTG CTACCTGCGT GTCGTGGGCA AAGGCAGCTA CGGGGAGGTG 
ACGCTTGTGA GGCACCGGCG GGACGGCAGG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CTCAGCTCTT GTCTCAGCTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCGTGGGAGG GTGGGGACGG TTTGCTGTAC 
ATCGTCATGG GCTTCTGTGA AGGAGGTGAT CTGTACCGAA AGCTCAAAGA GCAGAAAGGG 
AGGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTTC AGATCGCCAT GGCTCTGCAG 
TATTTACATG AAAAACACAT CCTCCACCGA GATCTAAAAA CCCAAAATGT CTTCCTGACA 
AGAACAAATA TCATCAAAGT GGGTGACCTT GGAATTGCCC GCGTGCTTGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCG TACTATATGA GCCCTGAACT GTTTTCGAAC 
AAACCCTACA ACTACAAGCT GCCACCAATG CCAAAAGATT ACAGCCCAGA GCTGGCAGAA 
CTGATAAGAA CTATGCTGAG CAAGAGACCT GAAGAAAGAC CTTCTGTGAG GAGCATCTTG 
AGGCAGCCTT ACATAAAGCG CCAAATATCC TTGTTTTTGG AGGCCACGAA GGCAAAAACC 
TCCAAAAATA ATGTTAAAAA TGGTGACTCT AAATCCAAGC CTGTGGCTGC AGTGGTTCCT 
GGAAAGGCTG AATTAAGTCA TGAAGTAGTT CCCCTTCAAT CACACTCTTC TGAGAGCTCC 
AAGACATATG TAATGGGTGA AGACAAATGT TTGTTCCAGG AGAAACCTGT AGTCATTGGC 
CCCTTGAAGA TACCCACAAG TCTGAAAGAC CACATCTACA AACCAGACAT AAGCAATACT 
GTGGACTCAC TAGCCACAAT CAGTAGGGTG AATATTGACA TCTTACCTAC AGAGAGGAGG 
AACTCAATGA ATGACGGCTT AGTTCAAGAG AACCCACCAG GGCACCTAGA TGCCTCTAAT 
GAACTGGAAG GTAAATGTAA TGTTTCTCAA GTGACGGAGA TGTTGCAGAA CAGTAAATCC 
AGTGCTCTAC CTGGAAACCG AATTCCCACA TTGTCCTCTC ACTGTGTCAC TGGAGAAAGG 
AATGACCCAA TGAAGCCTCT GCAGCCCCTA AACAAAGACC AAAATTTACC AAAAGACCAG 
GATCAAGTTG GTGGTGAATG TGTTACAGGA AAACAGGACA GAATCCACCC AGCTTTACAG 
CCACACAGCT CTGGGTCTGA ACCTTCCCAG TCTCGACAGC GGCGGCAGAA GAAAAGAGAA 
CACGCTGAGC ACAGTGGAGA AAAGAGACAG TTCCAAGAGA CTCCGCCTTG CCTTTTGCCT 
TCTCTTCCCA CTGTTGGAAA AATGGAATGT ACATCAACAC AAAAAGATGC TGAAAACCAA 
AGTAGAGTGG TCACTGGATC TGTGAACAGT TCGAGGGGCA GTGAGATAGC ATCATCAAAG 
GACCGACCAT TATCAGCAAG AGAGAGGAGG CGACTGAAAC AGTCACAGGA AGAGATCTTC 
CCCTCAGGCC CTGCAGTGAG GAGAGCTTCT CTGGGTCCAG CGGGACCAGC GAAACCACAG 
AAAGACCACC TCAGCCCTGC TCGAAGGTCA TCTTCCGACT GCAGTGTTGC TCAGGAAAGG 
AAGCTCACCC ATTGTCTCTC AGAGGATGAG TTAAGTTCCT CTACCAGTTC AACTGATAAA 
TCAGATGGGG ATTCCAGGGA AGGTAAAGGT CATACAAATG AAATGACTGA CTTGGTACAA 
TTGATGACTC AGACTCTGAA GTTGGACTCT AAAGAGAGCT ATGAAGATCT CCCAGTACCA 
GATCCAGTGT CAGAATTTAA ACTTCATCGG AAGTATCGGG ACACGCTAAT ACTTCATGGG 
AAAGTTGCAG AAGAGGCAGA GGAACTCCAT TTTAAAGAGC TACCTTCAGC GATCATGCCA 
GGTTCTGAAA AAATCAGAAG AATAGTTGAA GTCTTAAGAG CTGATGTAAT TCGGGGCCTG 
GGGATTCAGC TTTTAGAGCA GGTGTATGAT ATTTTGGAAG AGGAGGATGA ATTGGAAAGA 
GAGGTACATT TGCAGGAGCA CATGGGTGAA AAGTATGTGA CTTACAGTGT GAAAGCTCGC 
CAGTTAAAAT TTTTTGAAGA AAATGTGAAT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NEK4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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