DLC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DLC1
Entrez Gene ID	511433
Full Name	DLC1 Rho GTPase activating protein
Synonyms	DLC-1,StARD12
General protein information	Preferred Names DLC1 Rho GTPase activating protein Names rho GTPase-activating protein 7 START domain-containing protein 12 deleted in liver cancer 1 protein homolog rho-type GTPase-activating protein 7 stAR-related lipid transfer protein 12
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986238.1	XP_024842006.1	rho GTPase-activating protein 7 isoform X1
NM_001102493.3	NP_001095963.2	rho GTPase-activating protein 7
NM_001102493.1	NP_001095963.1	rho GTPase-activating protein 7
XM_024986241.1	XP_024842009.1	rho GTPase-activating protein 7 isoform X3
XM_024986240.1	XP_024842008.1	rho GTPase-activating protein 7 isoform X2
XM_024986239.1	XP_024842007.1	rho GTPase-activating protein 7 isoform X1
XM_024986237.1	XP_024842005.1	rho GTPase-activating protein 7 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DLC1	NP_872584.2
Pan troglodytes (chimpanzee)	DLC1	XP_001139210.2
Mus musculus (house mouse)	Dlc1	NP_001181869.1
Rattus norvegicus (Norway rat)	Dlc1	NP_001120918.1
Gallus gallus (chicken)	DLC1	XP_420693.3
	DLC1	XP_001092830.1
Canis lupus familiaris (dog)	DLC1	NP_001138543.1
Bos taurus (cattle)	DLC1	NP_001095963.1
Xenopus tropicalis (tropical clawed frog)	dlc1	XP_002934721.2
Danio rerio (zebrafish)	LOC567965	XP_005159917.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DLC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DLC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa261983	NM_001102493.3 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231341	XM_024986240.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231342	XM_024986241.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa44045	NM_001102493.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa231340	XM_024986237.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa231340	XM_024986239.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa231340	XM_024986238.1 Latest version!	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DLC1 ( NM_001102493.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095963.2 Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.

Price & Availability↑

CloneID	OBa261983
Clone ID Related Accession (Same CDS sequence)	NM_001102493.3
Accession Version	NM_001102493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151638.1 and NKLS02000027.1. On Aug 22, 2019 this sequence version replaced NM_001102493.2. ##Evidence-Data-START## Transcript exon combination :: BC151638.1, SRR5571254.44581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGACT CCGAGGCCCA CGTGATGGCG CGGCCCCTGA GAGCCCCTCT CCGGAGGTCC 
TTCAGTGATC ACATCCGAGA CTCCACGGCC AGAGCCCTGG ATGTCATTTG GAAAAACACC 
CGAGACAGGC GGCTGGCAGA AATCGAAGCC AAAGAAGCTT GTGATTGGCT CCGAGCCGCA 
GGATTCCCCC AGTATGCCCA GCTTTATGAA GATCTTCTGT TCCCCATTGA TATTTCCTCA 
GTCAAGAGAG AGCATGATTT TTTGGACAGA GATGCTATTG AGGCTTTATG CAGGCGTCTA 
AATACTTTAA ACAAATGTGC GGTGATGAAG CTGGAAATCA GTCCTCATCG GAAAAGAAGC 
GAAGATTCCG ACGAGGACGA GCCTTGTGCA ATAAGTGGCA AATGGACTTT CCAGAGGGAC 
AGCAAGAGGT GGTCCCGGCT TGAAGAGTTC GACGTCTTTT CTCCAAAACA GGACCCCATC 
CCTGGGTCCC CAGACGCTGT CCACCTGAAG AGCGCCCCGA GCCATGAAAA CATGCAGACG 
GACCTCAGCG ATCGCCAGGA GGTGGCTTCT GTCCACAGCA CCGGCAGCCT CACCACCCAC 
GCGCCCCAGC GCGGGGAGGC GGCCCCAGCC CGGACTAACT CCGTCCTCAG CGTCTGCTCC 
TCAGGCACCT TTGTAGGCAA TGATGATTCC TTCTGCAGCC TGCCCTCTCC CAAGGAACTG 
TCCAGCTTCA GCTTCAGCAT GAAGGGCCAC GAGAAGGCTG CCAAGTCCAA GACGCACAGC 
CTGCTGAAGC GCATGGAGAG CTTAAAGCTC AAGGGCTCCC ACCACAGCAA GCACAAGGCG 
CCTTCCAAGC TGGGTCTGAT CATCAGTGGG CCCATCCTGC AGGAGGGGGT GGACGAGGAG 
AAGCTGAAGC AGCTGAACTG CGTGGAGATC TCCGCCCTCA ACGGCAACCA CATCAACGTC 
CCCATGGTAC GGAAGAGGAG CATATCCAGC TCCACGCAGA CCAGCAGCAG CAGCAGCCAG 
TCTGAGACCA GCAGCAACGT GAGCACGCCC AGTCCCGTCA CCAGGACCCG GAGCCTCAGC 
GCCTGCAACA AGCGGGGAGG CATGTACTTG GAGGGCTTCG ATCCCTTCAA TCAGTCCACC 
TTCAACAACG TCATGGAGCA GAACTGTAAA AACCGGGAGA GCTACCCAGA GGACACAGTG 
TTCTACATCC CGGAAGATCA CAAGCCCGGC ACCTTCCCGA AAGCCCTTTC CAATGGCAGT 
TTCTCTCCCT CAGGGAATAA CAGCTCTGTG AACTGGAGGA CCGGGAGCTT CCACGGCCCC 
GGCCACATCA GCCTGAGGAG AGAAAACAGC AGCCCCAAGG AACTTAAGAG ACGTAACTCC 
TCGAGCTCTG TGAGCAGCCG CCTGAGTATC TACGACAACG TGCCAGGCTC CATCCTCTAT 
TCCAGCTCAG GTGACCTGGC CGATCTGGAG AACGAGGACA TCTTCCCTGA GCTGGACGAC 
ATCCTCTACC ATGTGAAGGG GATGCAGAGG ATAGTCAACC AGTGGTCAGA GAAGTTTTCC 
GATGAGGGAG ACTCCGACTC GGCCTTGGAC TCGGTCTCAC CATGCCCGTC CTCTCCCAAG 
CAGATACACC TGGATGTGGA CAATGACCGA GCCACCCCCA GTGACCTGGA CAGCACAGGC 
AACTCACTGA ATGAGCCTGA GGAGCCCTCT GACATCCCGG AAAGGAGGGA TTCTGGGGTC 
GGGGCCTCCC TGACCAGGTC CAACAGGCAC AGGCTGAGAT GGCACAGCTT CCAGAGCTCT 
CATCGGCCGA GCCTAAACTC CGTGTCACTG CAGATTAACT GCCAGTCAGT GGCCCAGATG 
AACCTGCTGC AGAAGTACTC CCTCTTGAAG TTAACCGCCC TGCTGGAGAA ATACACGCCT 
TCTAATAAGC ATGGCTTTAG CTGGGCTGTG CCCAAGTTCA TGAAAAGGAT CAAGGTTCCA 
GACTACAAGG ACCGGAATGT ATTCGGAGTC CCTCTGACAG TCAACGTGCA GCGCACAGGA 
CAGCCCCTCC CCCAGAGCAT CCAGCAGGCC ATGCGCTACC TCCGCAACCA TTGTTTGGAT 
CAGGTTGGAC TCTTCAGAAA ATCGGGTGTC AAATCCCGGA TTCAGGCTCT GCGCCAGATG 
AATGAAAGCA CCATAGACTG TGTCAACTAT GAGGGACAGT CTGCTTATGA CGTGGCAGAC 
ATGTTGAAGC AGTATTTTCG AGATCTGCCT GAGCCACTGA TGACAAACAA ACTCTCAGAA 
ACCTTTCTGC AGATATACCA ATATGTGCCC AAGGACCAGC GTCTCCAGGC GATCAAGGCG 
GCCATCATGC TCCTGCCCGA CGAGAACCGG GAGGTCCTAC AGACGCTCCT TTACTTCCTG 
AGCGATGTCA CCGCAGCCGT CAAGGAGAAC CAGATGACCC CCACCAACCT GGCCGTGTGC 
TTGGCGCCTT CTCTCTTCCA CCTCAACACT CTGAAGAGAG AGAACTCCTC CCCCAGGGTA 
ATGCAAAGAA AACAGAGTTT GGGCAAACCA GATCAAAAAG ATCTGAATGA GAATCTCGCT 
GCCACTCAAG GGCTAGCCCA TATGATTGCT GAGTGCAAGA AGCTTTTCCA GGTTCCTGAG 
GAAATGAGCC GATGTCGAAA TTCCTACACC GAACAGGAGC TAAAGCCCCT TACCCTCGAA 
GCGTTAGGAC GCCTTTGTAA TGATGACTCA GCCGACTACC AACACTTCCT CCAAGACTGT 
GTGGACAGCC TGTTTAAAGA GGTCAAGGAG AAGTTCAAAG GCTGGGTCAG TTACTCCACA 
TCTGAGCAGG CCGAGCTCTC TTATAAGAAG GTGAGTGAAG GACCACCTCT GAGGCTTTGG 
CGGGCAACCA TCGAAGTCCC TGCAACACCC GAGGAAATCT TAAAGCGCCT CCTGAAAGAG 
CAGCACCTCT GGGATGTAGA CTTGTTGGAT TCAAAAGTGA TTGAAATCCT GGACAGCCAG 
ACTGAAATTT ACCAGTATGT CCAAAACAGT ATGGCACCCC ATCCTGCTCG GGACTATGTC 
GTTTTGAGAA CATGGAGGAC TAATTTACCC AAGGGAGCCT GTGCCCTTTT ACTCACCTCG 
GTGGATCATG ACCGGGCCCC TGTGGTGGGA GTGAGGGTCA ATGTGCTCCT GGCCAGGTAT 
CTGATCGAAC CCTGTGGGTC AGGGAAATCC AAACTCACCT ACATGTGCAG AGCAGACTTA 
AGGGGCCACA TGCCAGAGTG GTACACGAAA TCTTTTGGAC ATTTGTGTGC AGCTGAAGTG 
GTAAAGATCC GAGACTCCTT CAGTCATCAG AACACTGAAA CCAAAGACAC CAAATCTAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095963.2
CDS	57..3419
Translation	MGDSEAHVMARPLRAPLRRSFSDHIRDSTARALDVIWKNTRDRRLAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	NM_001102493.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102493.3

ORF Insert Sequence:

ATGGGGGACT CCGAGGCCCA CGTGATGGCG CGGCCCCTGA GAGCCCCTCT CCGGAGGTCC 
TTCAGTGATC ACATCCGAGA CTCCACGGCC AGAGCCCTGG ATGTCATTTG GAAAAACACC 
CGAGACAGGC GGCTGGCAGA AATCGAAGCC AAAGAAGCTT GTGATTGGCT CCGAGCCGCA 
GGATTCCCCC AGTATGCCCA GCTTTATGAA GATCTTCTGT TCCCCATTGA TATTTCCTCA 
GTCAAGAGAG AGCATGATTT TTTGGACAGA GATGCTATTG AGGCTTTATG CAGGCGTCTA 
AATACTTTAA ACAAATGTGC GGTGATGAAG CTGGAAATCA GTCCTCATCG GAAAAGAAGC 
GAAGATTCCG ACGAGGACGA GCCTTGTGCA ATAAGTGGCA AATGGACTTT CCAGAGGGAC 
AGCAAGAGGT GGTCCCGGCT TGAAGAGTTC GACGTCTTTT CTCCAAAACA GGACCCCATC 
CCTGGGTCCC CAGACGCTGT CCACCTGAAG AGCGCCCCGA GCCATGAAAA CATGCAGACG 
GACCTCAGCG ATCGCCAGGA GGTGGCTTCT GTCCACAGCA CCGGCAGCCT CACCACCCAC 
GCGCCCCAGC GCGGGGAGGC GGCCCCAGCC CGGACTAACT CCGTCCTCAG CGTCTGCTCC 
TCAGGCACCT TTGTAGGCAA TGATGATTCC TTCTGCAGCC TGCCCTCTCC CAAGGAACTG 
TCCAGCTTCA GCTTCAGCAT GAAGGGCCAC GAGAAGGCTG CCAAGTCCAA GACGCACAGC 
CTGCTGAAGC GCATGGAGAG CTTAAAGCTC AAGGGCTCCC ACCACAGCAA GCACAAGGCG 
CCTTCCAAGC TGGGTCTGAT CATCAGTGGG CCCATCCTGC AGGAGGGGGT GGACGAGGAG 
AAGCTGAAGC AGCTGAACTG CGTGGAGATC TCCGCCCTCA ACGGCAACCA CATCAACGTC 
CCCATGGTAC GGAAGAGGAG CATATCCAGC TCCACGCAGA CCAGCAGCAG CAGCAGCCAG 
TCTGAGACCA GCAGCAACGT GAGCACGCCC AGTCCCGTCA CCAGGACCCG GAGCCTCAGC 
GCCTGCAACA AGCGGGGAGG CATGTACTTG GAGGGCTTCG ATCCCTTCAA TCAGTCCACC 
TTCAACAACG TCATGGAGCA GAACTGTAAA AACCGGGAGA GCTACCCAGA GGACACAGTG 
TTCTACATCC CGGAAGATCA CAAGCCCGGC ACCTTCCCGA AAGCCCTTTC CAATGGCAGT 
TTCTCTCCCT CAGGGAATAA CAGCTCTGTG AACTGGAGGA CCGGGAGCTT CCACGGCCCC 
GGCCACATCA GCCTGAGGAG AGAAAACAGC AGCCCCAAGG AACTTAAGAG ACGTAACTCC 
TCGAGCTCTG TGAGCAGCCG CCTGAGTATC TACGACAACG TGCCAGGCTC CATCCTCTAT 
TCCAGCTCAG GTGACCTGGC CGATCTGGAG AACGAGGACA TCTTCCCTGA GCTGGACGAC 
ATCCTCTACC ATGTGAAGGG GATGCAGAGG ATAGTCAACC AGTGGTCAGA GAAGTTTTCC 
GATGAGGGAG ACTCCGACTC GGCCTTGGAC TCGGTCTCAC CATGCCCGTC CTCTCCCAAG 
CAGATACACC TGGATGTGGA CAATGACCGA GCCACCCCCA GTGACCTGGA CAGCACAGGC 
AACTCACTGA ATGAGCCTGA GGAGCCCTCT GACATCCCGG AAAGGAGGGA TTCTGGGGTC 
GGGGCCTCCC TGACCAGGTC CAACAGGCAC AGGCTGAGAT GGCACAGCTT CCAGAGCTCT 
CATCGGCCGA GCCTAAACTC CGTGTCACTG CAGATTAACT GCCAGTCAGT GGCCCAGATG 
AACCTGCTGC AGAAGTACTC CCTCTTGAAG TTAACCGCCC TGCTGGAGAA ATACACGCCT 
TCTAATAAGC ATGGCTTTAG CTGGGCTGTG CCCAAGTTCA TGAAAAGGAT CAAGGTTCCA 
GACTACAAGG ACCGGAATGT ATTCGGAGTC CCTCTGACAG TCAACGTGCA GCGCACAGGA 
CAGCCCCTCC CCCAGAGCAT CCAGCAGGCC ATGCGCTACC TCCGCAACCA TTGTTTGGAT 
CAGGTTGGAC TCTTCAGAAA ATCGGGTGTC AAATCCCGGA TTCAGGCTCT GCGCCAGATG 
AATGAAAGCA CCATAGACTG TGTCAACTAT GAGGGACAGT CTGCTTATGA CGTGGCAGAC 
ATGTTGAAGC AGTATTTTCG AGATCTGCCT GAGCCACTGA TGACAAACAA ACTCTCAGAA 
ACCTTTCTGC AGATATACCA ATATGTGCCC AAGGACCAGC GTCTCCAGGC GATCAAGGCG 
GCCATCATGC TCCTGCCCGA CGAGAACCGG GAGGTCCTAC AGACGCTCCT TTACTTCCTG 
AGCGATGTCA CCGCAGCCGT CAAGGAGAAC CAGATGACCC CCACCAACCT GGCCGTGTGC 
TTGGCGCCTT CTCTCTTCCA CCTCAACACT CTGAAGAGAG AGAACTCCTC CCCCAGGGTA 
ATGCAAAGAA AACAGAGTTT GGGCAAACCA GATCAAAAAG ATCTGAATGA GAATCTCGCT 
GCCACTCAAG GGCTAGCCCA TATGATTGCT GAGTGCAAGA AGCTTTTCCA GGTTCCTGAG 
GAAATGAGCC GATGTCGAAA TTCCTACACC GAACAGGAGC TAAAGCCCCT TACCCTCGAA 
GCGTTAGGAC GCCTTTGTAA TGATGACTCA GCCGACTACC AACACTTCCT CCAAGACTGT 
GTGGACAGCC TGTTTAAAGA GGTCAAGGAG AAGTTCAAAG GCTGGGTCAG TTACTCCACA 
TCTGAGCAGG CCGAGCTCTC TTATAAGAAG GTGAGTGAAG GACCACCTCT GAGGCTTTGG 
CGGGCAACCA TCGAAGTCCC TGCAACACCC GAGGAAATCT TAAAGCGCCT CCTGAAAGAG 
CAGCACCTCT GGGATGTAGA CTTGTTGGAT TCAAAAGTGA TTGAAATCCT GGACAGCCAG 
ACTGAAATTT ACCAGTATGT CCAAAACAGT ATGGCACCCC ATCCTGCTCG GGACTATGTC 
GTTTTGAGAA CATGGAGGAC TAATTTACCC AAGGGAGCCT GTGCCCTTTT ACTCACCTCG 
GTGGATCATG ACCGGGCCCC TGTGGTGGGA GTGAGGGTCA ATGTGCTCCT GGCCAGGTAT 
CTGATCGAAC CCTGTGGGTC AGGGAAATCC AAACTCACCT ACATGTGCAG AGCAGACTTA 
AGGGGCCACA TGCCAGAGTG GTACACGAAA TCTTTTGGAC ATTTGTGTGC AGCTGAAGTG 
GTAAAGATCC GAGACTCCTT CAGTCATCAG AACACTGAAA CCAAAGACAC CAAATCTAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( XM_024986240.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842008.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa231341
Clone ID Related Accession (Same CDS sequence)	XM_024986240.1
Accession Version	XM_024986240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGAG AGAAGCCGGA CACCATGATC CTAACACGTA AGCTAGACTT GTGCCTTGCC 
GTCCGGCCGG CCTCGAGAGC CAGCTGCGGA GGGAACCCGC CGCGGAGGAA ATGTCACCCT 
GCCTTGTCAA GTCGGTGCCT GACGTTCTGT GCTTTGGCAG CGCTTTCCGA GGAGGCTCGG 
AACCGAGTCG CCCGTGTTTC TTGGAGTGCA GGAGAAATCG AAGCCAAAGA AGCTTGTGAT 
TGGCTCCGAG CCGCAGGATT CCCCCAGTAT GCCCAGCTTT ATGAAGATCT TCTGTTCCCC 
ATTGATATTT CCTCAGTCAA GAGAGAGCAT GATTTTTTGG ACAGAGATGC TATTGAGGCT 
TTATGCAGGC GTCTAAATAC TTTAAACAAA TGTGCGGTGA TGAAGCTGGA AATCAGTCCT 
CATCGGAAAA GAAGCGAAGA TTCCGACGAG GACGAGCCTT GTGCAATAAG TGGCAAATGG 
ACTTTCCAGA GGGACAGCAA GAGGTGGTCC CGGCTTGAAG AGTTCGACGT CTTTTCTCCA 
AAACAGGACC CCATCCCTGG GTCCCCAGAC GCTGTCCACC TGAAGAGCGC CCCGAGCCAT 
GAAAACATGC AGACGGACCT CAGCGATCGC CAGGAGGTGG CTTCTGTCCA CAGCACCGGC 
AGCCTCACCA CCCACGCGCC CCAGCGCGGG GAGGCGGCCC CAGCCCGGAC TAACTCCGTC 
CTCAGCGTCT GCTCCTCAGG CACCTTTGTA GGCAATGATG ATTCCTTCTG CAGCCTGCCC 
TCTCCCAAGG AACTGTCCAG CTTCAGCTTC AGCATGAAGG GCCACGAGAA GGCTGCCAAG 
TCCAAGACGC ACAGCCTGCT GAAGCGCATG GAGAGCTTAA AGCTCAAGGG CTCCCACCAC 
AGCAAGCACA AGGCGCCTTC CAAGCTGGGT CTGATCATCA GTGGGCCCAT CCTGCAGGAG 
GGGGTGGACG AGGAGAAGCT GAAGCAGCTG AACTGCGTGG AGATCTCCGC CCTCAACGGC 
AACCACATCA ACGTCCCCAT GGTACGGAAG AGGAGCATAT CCAGCTCCAC GCAGACCAGC 
AGCAGCAGCA GCCAGTCTGA GACCAGCAGC AACGTGAGCA CGCCCAGTCC CGTCACCAGG 
ACCCGGAGCC TCAGCGCCTG CAACAAGCGG GGAGGCATGT ACTTGGAGGG CTTCGATCCC 
TTCAATCAGT CCACCTTCAA CAACGTCATG GAGCAGAACT GTAAAAACCG GGAGAGCTAC 
CCAGAGGACA CAGTGTTCTA CATCCCGGAA GATCACAAGC CCGGCACCTT CCCGAAAGCC 
CTTTCCAATG GCAGTTTCTC TCCCTCAGGG AATAACAGCT CTGTGAACTG GAGGACCGGG 
AGCTTCCACG GCCCCGGCCA CATCAGCCTG AGGAGAGAAA ACAGCAGCCC CAAGGAACTT 
AAGAGACGTA ACTCCTCGAG CTCTGTGAGC AGCCGCCTGA GTATCTACGA CAACGTGCCA 
GGCTCCATCC TCTATTCCAG CTCAGGTGAC CTGGCCGATC TGGAGAACGA GGACATCTTC 
CCTGAGCTGG ACGACATCCT CTACCATGTG AAGGGGATGC AGAGGATAGT CAACCAGTGG 
TCAGAGAAGT TTTCCGATGA GGGAGACTCC GACTCGGCCT TGGACTCGGT CTCACCATGC 
CCGTCCTCTC CCAAGCAGAT ACACCTGGAT GTGGACAATG ACCGAGCCAC CCCCAGTGAC 
CTGGACAGCA CAGGCAACTC ACTGAATGAG CCTGAGGAGC CCTCTGACAT CCCGGAAAGG 
AGGGATTCTG GGGTCGGGGC CTCCCTGACC AGGTCCAACA GGCACAGGCT GAGATGGCAC 
AGCTTCCAGA GCTCTCATCG GCCGAGCCTA AACTCCGTGT CACTGCAGAT TAACTGCCAG 
TCAGTGGCCC AGATGAACCT GCTGCAGAAG TACTCCCTCT TGAAGTTAAC CGCCCTGCTG 
GAGAAATACA CGCCTTCTAA TAAGCATGGC TTTAGCTGGG CTGTGCCCAA GTTCATGAAA 
AGGATCAAGG TTCCAGACTA CAAGGACCGG AATGTATTCG GAGTCCCTCT GACAGTCAAC 
GTGCAGCGCA CAGGACAGCC CCTCCCCCAG AGCATCCAGC AGGCCATGCG CTACCTCCGC 
AACCATTGTT TGGATCAGGT TGGACTCTTC AGAAAATCGG GTGTCAAATC CCGGATTCAG 
GCTCTGCGCC AGATGAATGA AAGCACCATA GACTGTGTCA ACTATGAGGG ACAGTCTGCT 
TATGACGTGG CAGACATGTT GAAGCAGTAT TTTCGAGATC TGCCTGAGCC ACTGATGACA 
AACAAACTCT CAGAAACCTT TCTGCAGATA TACCAATATG TGCCCAAGGA CCAGCGTCTC 
CAGGCGATCA AGGCGGCCAT CATGCTCCTG CCCGACGAGA ACCGGGAGGT CCTACAGACG 
CTCCTTTACT TCCTGAGCGA TGTCACCGCA GCCGTCAAGG AGAACCAGAT GACCCCCACC 
AACCTGGCCG TGTGCTTGGC GCCTTCTCTC TTCCACCTCA ACACTCTGAA GAGAGAGAAC 
TCCTCCCCCA GGGTAATGCA AAGAAAACAG AGTTTGGGCA AACCAGATCA AAAAGATCTG 
AATGAGAATC TCGCTGCCAC TCAAGGGCTA GCCCATATGA TTGCTGAGTG CAAGAAGCTT 
TTCCAGGTTC CTGAGGAAAT GAGCCGATGT CGAAATTCCT ACACCGAACA GGAGCTAAAG 
CCCCTTACCC TCGAAGCGTT AGGACGCCTT TGTAATGATG ACTCAGCCGA CTACCAACAC 
TTCCTCCAAG ACTGTGTGGA CAGCCTGTTT AAAGAGGTCA AGGAGAAGTT CAAAGGCTGG 
GTCAGTTACT CCACATCTGA GCAGGCCGAG CTCTCTTATA AGAAGGTGAG TGAAGGACCA 
CCTCTGAGGC TTTGGCGGGC AACCATCGAA GTCCCTGCAA CACCCGAGGA AATCTTAAAG 
CGCCTCCTGA AAGAGCAGCA CCTCTGGGAT GTAGACTTGT TGGATTCAAA AGTGATTGAA 
ATCCTGGACA GCCAGACTGA AATTTACCAG TATGTCCAAA ACAGTATGGC ACCCCATCCT 
GCTCGGGACT ATGTCGTTTT GAGAACATGG AGGACTAATT TACCCAAGGG AGCCTGTGCC 
CTTTTACTCA CCTCGGTGGA TCATGACCGG GCCCCTGTGG TGGGAGTGAG GGTCAATGTG 
CTCCTGGCCA GGTATCTGAT CGAACCCTGT GGGTCAGGGA AATCCAAACT CACCTACATG 
TGCAGAGCAG ACTTAAGGGG CCACATGCCA GAGTGGTACA CGAAATCTTT TGGACATTTG 
TGTGCAGCTG AAGTGGTAAA GATCCGAGAC TCCTTCAGTC ATCAGAACAC TGAAACCAAA 
GACACCAAAT CTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842008.1
CDS	327..3764
Translation	MCREKPDTMILTRKLDLCLAVRPASRASCGGNPPRRKCHPALSSRCLTFCALAALSEEARNRVARVSWSAGEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	XM_024986240.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986240.1

ORF Insert Sequence:

ATGTGCAGAG AGAAGCCGGA CACCATGATC CTAACACGTA AGCTAGACTT GTGCCTTGCC 
GTCCGGCCGG CCTCGAGAGC CAGCTGCGGA GGGAACCCGC CGCGGAGGAA ATGTCACCCT 
GCCTTGTCAA GTCGGTGCCT GACGTTCTGT GCTTTGGCAG CGCTTTCCGA GGAGGCTCGG 
AACCGAGTCG CCCGTGTTTC TTGGAGTGCA GGAGAAATCG AAGCCAAAGA AGCTTGTGAT 
TGGCTCCGAG CCGCAGGATT CCCCCAGTAT GCCCAGCTTT ATGAAGATCT TCTGTTCCCC 
ATTGATATTT CCTCAGTCAA GAGAGAGCAT GATTTTTTGG ACAGAGATGC TATTGAGGCT 
TTATGCAGGC GTCTAAATAC TTTAAACAAA TGTGCGGTGA TGAAGCTGGA AATCAGTCCT 
CATCGGAAAA GAAGCGAAGA TTCCGACGAG GACGAGCCTT GTGCAATAAG TGGCAAATGG 
ACTTTCCAGA GGGACAGCAA GAGGTGGTCC CGGCTTGAAG AGTTCGACGT CTTTTCTCCA 
AAACAGGACC CCATCCCTGG GTCCCCAGAC GCTGTCCACC TGAAGAGCGC CCCGAGCCAT 
GAAAACATGC AGACGGACCT CAGCGATCGC CAGGAGGTGG CTTCTGTCCA CAGCACCGGC 
AGCCTCACCA CCCACGCGCC CCAGCGCGGG GAGGCGGCCC CAGCCCGGAC TAACTCCGTC 
CTCAGCGTCT GCTCCTCAGG CACCTTTGTA GGCAATGATG ATTCCTTCTG CAGCCTGCCC 
TCTCCCAAGG AACTGTCCAG CTTCAGCTTC AGCATGAAGG GCCACGAGAA GGCTGCCAAG 
TCCAAGACGC ACAGCCTGCT GAAGCGCATG GAGAGCTTAA AGCTCAAGGG CTCCCACCAC 
AGCAAGCACA AGGCGCCTTC CAAGCTGGGT CTGATCATCA GTGGGCCCAT CCTGCAGGAG 
GGGGTGGACG AGGAGAAGCT GAAGCAGCTG AACTGCGTGG AGATCTCCGC CCTCAACGGC 
AACCACATCA ACGTCCCCAT GGTACGGAAG AGGAGCATAT CCAGCTCCAC GCAGACCAGC 
AGCAGCAGCA GCCAGTCTGA GACCAGCAGC AACGTGAGCA CGCCCAGTCC CGTCACCAGG 
ACCCGGAGCC TCAGCGCCTG CAACAAGCGG GGAGGCATGT ACTTGGAGGG CTTCGATCCC 
TTCAATCAGT CCACCTTCAA CAACGTCATG GAGCAGAACT GTAAAAACCG GGAGAGCTAC 
CCAGAGGACA CAGTGTTCTA CATCCCGGAA GATCACAAGC CCGGCACCTT CCCGAAAGCC 
CTTTCCAATG GCAGTTTCTC TCCCTCAGGG AATAACAGCT CTGTGAACTG GAGGACCGGG 
AGCTTCCACG GCCCCGGCCA CATCAGCCTG AGGAGAGAAA ACAGCAGCCC CAAGGAACTT 
AAGAGACGTA ACTCCTCGAG CTCTGTGAGC AGCCGCCTGA GTATCTACGA CAACGTGCCA 
GGCTCCATCC TCTATTCCAG CTCAGGTGAC CTGGCCGATC TGGAGAACGA GGACATCTTC 
CCTGAGCTGG ACGACATCCT CTACCATGTG AAGGGGATGC AGAGGATAGT CAACCAGTGG 
TCAGAGAAGT TTTCCGATGA GGGAGACTCC GACTCGGCCT TGGACTCGGT CTCACCATGC 
CCGTCCTCTC CCAAGCAGAT ACACCTGGAT GTGGACAATG ACCGAGCCAC CCCCAGTGAC 
CTGGACAGCA CAGGCAACTC ACTGAATGAG CCTGAGGAGC CCTCTGACAT CCCGGAAAGG 
AGGGATTCTG GGGTCGGGGC CTCCCTGACC AGGTCCAACA GGCACAGGCT GAGATGGCAC 
AGCTTCCAGA GCTCTCATCG GCCGAGCCTA AACTCCGTGT CACTGCAGAT TAACTGCCAG 
TCAGTGGCCC AGATGAACCT GCTGCAGAAG TACTCCCTCT TGAAGTTAAC CGCCCTGCTG 
GAGAAATACA CGCCTTCTAA TAAGCATGGC TTTAGCTGGG CTGTGCCCAA GTTCATGAAA 
AGGATCAAGG TTCCAGACTA CAAGGACCGG AATGTATTCG GAGTCCCTCT GACAGTCAAC 
GTGCAGCGCA CAGGACAGCC CCTCCCCCAG AGCATCCAGC AGGCCATGCG CTACCTCCGC 
AACCATTGTT TGGATCAGGT TGGACTCTTC AGAAAATCGG GTGTCAAATC CCGGATTCAG 
GCTCTGCGCC AGATGAATGA AAGCACCATA GACTGTGTCA ACTATGAGGG ACAGTCTGCT 
TATGACGTGG CAGACATGTT GAAGCAGTAT TTTCGAGATC TGCCTGAGCC ACTGATGACA 
AACAAACTCT CAGAAACCTT TCTGCAGATA TACCAATATG TGCCCAAGGA CCAGCGTCTC 
CAGGCGATCA AGGCGGCCAT CATGCTCCTG CCCGACGAGA ACCGGGAGGT CCTACAGACG 
CTCCTTTACT TCCTGAGCGA TGTCACCGCA GCCGTCAAGG AGAACCAGAT GACCCCCACC 
AACCTGGCCG TGTGCTTGGC GCCTTCTCTC TTCCACCTCA ACACTCTGAA GAGAGAGAAC 
TCCTCCCCCA GGGTAATGCA AAGAAAACAG AGTTTGGGCA AACCAGATCA AAAAGATCTG 
AATGAGAATC TCGCTGCCAC TCAAGGGCTA GCCCATATGA TTGCTGAGTG CAAGAAGCTT 
TTCCAGGTTC CTGAGGAAAT GAGCCGATGT CGAAATTCCT ACACCGAACA GGAGCTAAAG 
CCCCTTACCC TCGAAGCGTT AGGACGCCTT TGTAATGATG ACTCAGCCGA CTACCAACAC 
TTCCTCCAAG ACTGTGTGGA CAGCCTGTTT AAAGAGGTCA AGGAGAAGTT CAAAGGCTGG 
GTCAGTTACT CCACATCTGA GCAGGCCGAG CTCTCTTATA AGAAGGTGAG TGAAGGACCA 
CCTCTGAGGC TTTGGCGGGC AACCATCGAA GTCCCTGCAA CACCCGAGGA AATCTTAAAG 
CGCCTCCTGA AAGAGCAGCA CCTCTGGGAT GTAGACTTGT TGGATTCAAA AGTGATTGAA 
ATCCTGGACA GCCAGACTGA AATTTACCAG TATGTCCAAA ACAGTATGGC ACCCCATCCT 
GCTCGGGACT ATGTCGTTTT GAGAACATGG AGGACTAATT TACCCAAGGG AGCCTGTGCC 
CTTTTACTCA CCTCGGTGGA TCATGACCGG GCCCCTGTGG TGGGAGTGAG GGTCAATGTG 
CTCCTGGCCA GGTATCTGAT CGAACCCTGT GGGTCAGGGA AATCCAAACT CACCTACATG 
TGCAGAGCAG ACTTAAGGGG CCACATGCCA GAGTGGTACA CGAAATCTTT TGGACATTTG 
TGTGCAGCTG AAGTGGTAAA GATCCGAGAC TCCTTCAGTC ATCAGAACAC TGAAACCAAA 
GACACCAAAT CTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( XM_024986241.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842009.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa231342
Clone ID Related Accession (Same CDS sequence)	XM_024986241.1
Accession Version	XM_024986241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGAG AGAAGCCGGA CACCATGATC CTAACACAAA TCGAAGCCAA AGAAGCTTGT 
GATTGGCTCC GAGCCGCAGG ATTCCCCCAG TATGCCCAGC TTTATGAAGA TCTTCTGTTC 
CCCATTGATA TTTCCTCAGT CAAGAGAGAG CATGATTTTT TGGACAGAGA TGCTATTGAG 
GCTTTATGCA GGCGTCTAAA TACTTTAAAC AAATGTGCGG TGATGAAGCT GGAAATCAGT 
CCTCATCGGA AAAGAAGCGA AGATTCCGAC GAGGACGAGC CTTGTGCAAT AAGTGGCAAA 
TGGACTTTCC AGAGGGACAG CAAGAGGTGG TCCCGGCTTG AAGAGTTCGA CGTCTTTTCT 
CCAAAACAGG ACCCCATCCC TGGGTCCCCA GACGCTGTCC ACCTGAAGAG CGCCCCGAGC 
CATGAAAACA TGCAGACGGA CCTCAGCGAT CGCCAGGAGG TGGCTTCTGT CCACAGCACC 
GGCAGCCTCA CCACCCACGC GCCCCAGCGC GGGGAGGCGG CCCCAGCCCG GACTAACTCC 
GTCCTCAGCG TCTGCTCCTC AGGCACCTTT GTAGGCAATG ATGATTCCTT CTGCAGCCTG 
CCCTCTCCCA AGGAACTGTC CAGCTTCAGC TTCAGCATGA AGGGCCACGA GAAGGCTGCC 
AAGTCCAAGA CGCACAGCCT GCTGAAGCGC ATGGAGAGCT TAAAGCTCAA GGGCTCCCAC 
CACAGCAAGC ACAAGGCGCC TTCCAAGCTG GGTCTGATCA TCAGTGGGCC CATCCTGCAG 
GAGGGGGTGG ACGAGGAGAA GCTGAAGCAG CTGAACTGCG TGGAGATCTC CGCCCTCAAC 
GGCAACCACA TCAACGTCCC CATGGTACGG AAGAGGAGCA TATCCAGCTC CACGCAGACC 
AGCAGCAGCA GCAGCCAGTC TGAGACCAGC AGCAACGTGA GCACGCCCAG TCCCGTCACC 
AGGACCCGGA GCCTCAGCGC CTGCAACAAG CGGGGAGGCA TGTACTTGGA GGGCTTCGAT 
CCCTTCAATC AGTCCACCTT CAACAACGTC ATGGAGCAGA ACTGTAAAAA CCGGGAGAGC 
TACCCAGAGG ACACAGTGTT CTACATCCCG GAAGATCACA AGCCCGGCAC CTTCCCGAAA 
GCCCTTTCCA ATGGCAGTTT CTCTCCCTCA GGGAATAACA GCTCTGTGAA CTGGAGGACC 
GGGAGCTTCC ACGGCCCCGG CCACATCAGC CTGAGGAGAG AAAACAGCAG CCCCAAGGAA 
CTTAAGAGAC GTAACTCCTC GAGCTCTGTG AGCAGCCGCC TGAGTATCTA CGACAACGTG 
CCAGGCTCCA TCCTCTATTC CAGCTCAGGT GACCTGGCCG ATCTGGAGAA CGAGGACATC 
TTCCCTGAGC TGGACGACAT CCTCTACCAT GTGAAGGGGA TGCAGAGGAT AGTCAACCAG 
TGGTCAGAGA AGTTTTCCGA TGAGGGAGAC TCCGACTCGG CCTTGGACTC GGTCTCACCA 
TGCCCGTCCT CTCCCAAGCA GATACACCTG GATGTGGACA ATGACCGAGC CACCCCCAGT 
GACCTGGACA GCACAGGCAA CTCACTGAAT GAGCCTGAGG AGCCCTCTGA CATCCCGGAA 
AGGAGGGATT CTGGGGTCGG GGCCTCCCTG ACCAGGTCCA ACAGGCACAG GCTGAGATGG 
CACAGCTTCC AGAGCTCTCA TCGGCCGAGC CTAAACTCCG TGTCACTGCA GATTAACTGC 
CAGTCAGTGG CCCAGATGAA CCTGCTGCAG AAGTACTCCC TCTTGAAGTT AACCGCCCTG 
CTGGAGAAAT ACACGCCTTC TAATAAGCAT GGCTTTAGCT GGGCTGTGCC CAAGTTCATG 
AAAAGGATCA AGGTTCCAGA CTACAAGGAC CGGAATGTAT TCGGAGTCCC TCTGACAGTC 
AACGTGCAGC GCACAGGACA GCCCCTCCCC CAGAGCATCC AGCAGGCCAT GCGCTACCTC 
CGCAACCATT GTTTGGATCA GGTTGGACTC TTCAGAAAAT CGGGTGTCAA ATCCCGGATT 
CAGGCTCTGC GCCAGATGAA TGAAAGCACC ATAGACTGTG TCAACTATGA GGGACAGTCT 
GCTTATGACG TGGCAGACAT GTTGAAGCAG TATTTTCGAG ATCTGCCTGA GCCACTGATG 
ACAAACAAAC TCTCAGAAAC CTTTCTGCAG ATATACCAAT ATGTGCCCAA GGACCAGCGT 
CTCCAGGCGA TCAAGGCGGC CATCATGCTC CTGCCCGACG AGAACCGGGA GGTCCTACAG 
ACGCTCCTTT ACTTCCTGAG CGATGTCACC GCAGCCGTCA AGGAGAACCA GATGACCCCC 
ACCAACCTGG CCGTGTGCTT GGCGCCTTCT CTCTTCCACC TCAACACTCT GAAGAGAGAG 
AACTCCTCCC CCAGGGTAAT GCAAAGAAAA CAGAGTTTGG GCAAACCAGA TCAAAAAGAT 
CTGAATGAGA ATCTCGCTGC CACTCAAGGG CTAGCCCATA TGATTGCTGA GTGCAAGAAG 
CTTTTCCAGG TTCCTGAGGA AATGAGCCGA TGTCGAAATT CCTACACCGA ACAGGAGCTA 
AAGCCCCTTA CCCTCGAAGC GTTAGGACGC CTTTGTAATG ATGACTCAGC CGACTACCAA 
CACTTCCTCC AAGACTGTGT GGACAGCCTG TTTAAAGAGG TCAAGGAGAA GTTCAAAGGC 
TGGGTCAGTT ACTCCACATC TGAGCAGGCC GAGCTCTCTT ATAAGAAGGT GAGTGAAGGA 
CCACCTCTGA GGCTTTGGCG GGCAACCATC GAAGTCCCTG CAACACCCGA GGAAATCTTA 
AAGCGCCTCC TGAAAGAGCA GCACCTCTGG GATGTAGACT TGTTGGATTC AAAAGTGATT 
GAAATCCTGG ACAGCCAGAC TGAAATTTAC CAGTATGTCC AAAACAGTAT GGCACCCCAT 
CCTGCTCGGG ACTATGTCGT TTTGAGAACA TGGAGGACTA ATTTACCCAA GGGAGCCTGT 
GCCCTTTTAC TCACCTCGGT GGATCATGAC CGGGCCCCTG TGGTGGGAGT GAGGGTCAAT 
GTGCTCCTGG CCAGGTATCT GATCGAACCC TGTGGGTCAG GGAAATCCAA ACTCACCTAC 
ATGTGCAGAG CAGACTTAAG GGGCCACATG CCAGAGTGGT ACACGAAATC TTTTGGACAT 
TTGTGTGCAG CTGAAGTGGT AAAGATCCGA GACTCCTTCA GTCATCAGAA CACTGAAACC 
AAAGACACCA AATCTAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842009.1
CDS	334..3594
Translation	MCREKPDTMILTQIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	XM_024986241.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986241.1

ORF Insert Sequence:

ATGTGCAGAG AGAAGCCGGA CACCATGATC CTAACACAAA TCGAAGCCAA AGAAGCTTGT 
GATTGGCTCC GAGCCGCAGG ATTCCCCCAG TATGCCCAGC TTTATGAAGA TCTTCTGTTC 
CCCATTGATA TTTCCTCAGT CAAGAGAGAG CATGATTTTT TGGACAGAGA TGCTATTGAG 
GCTTTATGCA GGCGTCTAAA TACTTTAAAC AAATGTGCGG TGATGAAGCT GGAAATCAGT 
CCTCATCGGA AAAGAAGCGA AGATTCCGAC GAGGACGAGC CTTGTGCAAT AAGTGGCAAA 
TGGACTTTCC AGAGGGACAG CAAGAGGTGG TCCCGGCTTG AAGAGTTCGA CGTCTTTTCT 
CCAAAACAGG ACCCCATCCC TGGGTCCCCA GACGCTGTCC ACCTGAAGAG CGCCCCGAGC 
CATGAAAACA TGCAGACGGA CCTCAGCGAT CGCCAGGAGG TGGCTTCTGT CCACAGCACC 
GGCAGCCTCA CCACCCACGC GCCCCAGCGC GGGGAGGCGG CCCCAGCCCG GACTAACTCC 
GTCCTCAGCG TCTGCTCCTC AGGCACCTTT GTAGGCAATG ATGATTCCTT CTGCAGCCTG 
CCCTCTCCCA AGGAACTGTC CAGCTTCAGC TTCAGCATGA AGGGCCACGA GAAGGCTGCC 
AAGTCCAAGA CGCACAGCCT GCTGAAGCGC ATGGAGAGCT TAAAGCTCAA GGGCTCCCAC 
CACAGCAAGC ACAAGGCGCC TTCCAAGCTG GGTCTGATCA TCAGTGGGCC CATCCTGCAG 
GAGGGGGTGG ACGAGGAGAA GCTGAAGCAG CTGAACTGCG TGGAGATCTC CGCCCTCAAC 
GGCAACCACA TCAACGTCCC CATGGTACGG AAGAGGAGCA TATCCAGCTC CACGCAGACC 
AGCAGCAGCA GCAGCCAGTC TGAGACCAGC AGCAACGTGA GCACGCCCAG TCCCGTCACC 
AGGACCCGGA GCCTCAGCGC CTGCAACAAG CGGGGAGGCA TGTACTTGGA GGGCTTCGAT 
CCCTTCAATC AGTCCACCTT CAACAACGTC ATGGAGCAGA ACTGTAAAAA CCGGGAGAGC 
TACCCAGAGG ACACAGTGTT CTACATCCCG GAAGATCACA AGCCCGGCAC CTTCCCGAAA 
GCCCTTTCCA ATGGCAGTTT CTCTCCCTCA GGGAATAACA GCTCTGTGAA CTGGAGGACC 
GGGAGCTTCC ACGGCCCCGG CCACATCAGC CTGAGGAGAG AAAACAGCAG CCCCAAGGAA 
CTTAAGAGAC GTAACTCCTC GAGCTCTGTG AGCAGCCGCC TGAGTATCTA CGACAACGTG 
CCAGGCTCCA TCCTCTATTC CAGCTCAGGT GACCTGGCCG ATCTGGAGAA CGAGGACATC 
TTCCCTGAGC TGGACGACAT CCTCTACCAT GTGAAGGGGA TGCAGAGGAT AGTCAACCAG 
TGGTCAGAGA AGTTTTCCGA TGAGGGAGAC TCCGACTCGG CCTTGGACTC GGTCTCACCA 
TGCCCGTCCT CTCCCAAGCA GATACACCTG GATGTGGACA ATGACCGAGC CACCCCCAGT 
GACCTGGACA GCACAGGCAA CTCACTGAAT GAGCCTGAGG AGCCCTCTGA CATCCCGGAA 
AGGAGGGATT CTGGGGTCGG GGCCTCCCTG ACCAGGTCCA ACAGGCACAG GCTGAGATGG 
CACAGCTTCC AGAGCTCTCA TCGGCCGAGC CTAAACTCCG TGTCACTGCA GATTAACTGC 
CAGTCAGTGG CCCAGATGAA CCTGCTGCAG AAGTACTCCC TCTTGAAGTT AACCGCCCTG 
CTGGAGAAAT ACACGCCTTC TAATAAGCAT GGCTTTAGCT GGGCTGTGCC CAAGTTCATG 
AAAAGGATCA AGGTTCCAGA CTACAAGGAC CGGAATGTAT TCGGAGTCCC TCTGACAGTC 
AACGTGCAGC GCACAGGACA GCCCCTCCCC CAGAGCATCC AGCAGGCCAT GCGCTACCTC 
CGCAACCATT GTTTGGATCA GGTTGGACTC TTCAGAAAAT CGGGTGTCAA ATCCCGGATT 
CAGGCTCTGC GCCAGATGAA TGAAAGCACC ATAGACTGTG TCAACTATGA GGGACAGTCT 
GCTTATGACG TGGCAGACAT GTTGAAGCAG TATTTTCGAG ATCTGCCTGA GCCACTGATG 
ACAAACAAAC TCTCAGAAAC CTTTCTGCAG ATATACCAAT ATGTGCCCAA GGACCAGCGT 
CTCCAGGCGA TCAAGGCGGC CATCATGCTC CTGCCCGACG AGAACCGGGA GGTCCTACAG 
ACGCTCCTTT ACTTCCTGAG CGATGTCACC GCAGCCGTCA AGGAGAACCA GATGACCCCC 
ACCAACCTGG CCGTGTGCTT GGCGCCTTCT CTCTTCCACC TCAACACTCT GAAGAGAGAG 
AACTCCTCCC CCAGGGTAAT GCAAAGAAAA CAGAGTTTGG GCAAACCAGA TCAAAAAGAT 
CTGAATGAGA ATCTCGCTGC CACTCAAGGG CTAGCCCATA TGATTGCTGA GTGCAAGAAG 
CTTTTCCAGG TTCCTGAGGA AATGAGCCGA TGTCGAAATT CCTACACCGA ACAGGAGCTA 
AAGCCCCTTA CCCTCGAAGC GTTAGGACGC CTTTGTAATG ATGACTCAGC CGACTACCAA 
CACTTCCTCC AAGACTGTGT GGACAGCCTG TTTAAAGAGG TCAAGGAGAA GTTCAAAGGC 
TGGGTCAGTT ACTCCACATC TGAGCAGGCC GAGCTCTCTT ATAAGAAGGT GAGTGAAGGA 
CCACCTCTGA GGCTTTGGCG GGCAACCATC GAAGTCCCTG CAACACCCGA GGAAATCTTA 
AAGCGCCTCC TGAAAGAGCA GCACCTCTGG GATGTAGACT TGTTGGATTC AAAAGTGATT 
GAAATCCTGG ACAGCCAGAC TGAAATTTAC CAGTATGTCC AAAACAGTAT GGCACCCCAT 
CCTGCTCGGG ACTATGTCGT TTTGAGAACA TGGAGGACTA ATTTACCCAA GGGAGCCTGT 
GCCCTTTTAC TCACCTCGGT GGATCATGAC CGGGCCCCTG TGGTGGGAGT GAGGGTCAAT 
GTGCTCCTGG CCAGGTATCT GATCGAACCC TGTGGGTCAG GGAAATCCAA ACTCACCTAC 
ATGTGCAGAG CAGACTTAAG GGGCCACATG CCAGAGTGGT ACACGAAATC TTTTGGACAT 
TTGTGTGCAG CTGAAGTGGT AAAGATCCGA GACTCCTTCA GTCATCAGAA CACTGAAACC 
AAAGACACCA AATCTAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( NM_001102493.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095963.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.

Price & Availability↑

CloneID	OBa44045
Clone ID Related Accession (Same CDS sequence)	NM_001102493.1
Accession Version	NM_001102493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151638.1. On Aug 29, 2007 this sequence version replaced XR_027491.1. ##Evidence-Data-START## Transcript exon combination :: BC151638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGGC CCCTGAGAGC CCCTCTCCGG AGGTCCTTCA GTGATCACAT CCGAGACTCC 
ACGGCCAGAG CCCTGGATGT CATTTGGAAA AACACCCGAG ACAGGCGGCT GGCAGAAATC 
GAAGCCAAAG AAGCTTGTGA TTGGCTCCGA GCCGCAGGAT TCCCCCAGTA TGCCCAGCTT 
TATGAAGATC TTCTGTTCCC CATTGATATT TCCTCAGTCA AGAGAGAGCA TGATTTTTTG 
GACAGAGATG CTATTGAGGC TTTATGCAGG CGTCTAAATA CTTTAAACAA ATGTGCAGTG 
ATGAAGCTGG AAATCAGTCC TCATCGGAAA AGAAGCGAAG ATTCCGACGA GGACGAGCCT 
TGTGCAATAA GTGGCAAATG GACTTTCCAG AGGGACAGCA AGAGGTGGTC CCGGCTTGAA 
GAGTTCGACG TCTTTTCTCC AAAACAGGAC CCCATCCCTG GGTCCCCAGA CGCTGTCCAC 
CTGAAGAGCG CCCCGAGCCA TGAAAACATG CAGACGGACC TCAGCGATCG CCAGGAGGTG 
GCTTCTGTCC ACAGCACCGG CAGCCTCACC ACCCACGCGC CCCAGCGCGG GGAGGCGGCC 
CCAGCCCGGA CTAACTCCGT CCTCAGCGTC TGCTCCTCAG GCACCTTTGT AGGCAATGAT 
GATTCCTTCT GCAGCCTGCC CTCTCCCAAG GAACTGTCCA GCTTCAGCTT CAGCATGAAG 
GGCCACGAGA AGGCTGCCAA GTCCAAGACG CACAGCCTGC TGAAGCGCAT GGAGAGCTTA 
AAGCTCAAGG GCTCCCACCA CAGCAAGCAC AAGGCGCCTT CCAAGCTGGG TCTGATCATC 
AGTGGGCCCA TCCTGCAGGA GGGGGTGGAC GAGGAGAAGC TGAAGCAGCT GAACTGCGTG 
GAGATCTCCG CCCTCAACGG CAACCACATC AACGTCCCCA TGGTACGGAA GAGGAGCATA 
TCCAGCTCCA CGCAGACCAG CAGCAGCAGC AGCCAGTCTG AGACCAGCAG CAACGTGAGC 
ACGCCCAGTC CCGTCACCAG GACCCGGAGC CTCAGCGCCT GCAACAAGCG GGGAGGCATG 
TACTTGGAGG GCTTCGATCC CTTCAATCAG TCCACCTTCA ACAACGTCAT GGAGCAGAAC 
TGTAAAAACC GGGAGAGCTA CCCAGAGGAC ACAGTGTTCT ACATCCCGGA AGATCACAAG 
CCCGGCACCT TCCCGAAAGC CCTTTCCAAT GGCAGTTTCT CTCCCTCAGG GAATAACAGC 
TCTGTGAACT GGAGGACCGG GAGCTTCCAC GGCCCCGGCC ACATCAGCCT GAGGAGAGAA 
AACAGCAGCC CCAAGGAACT TAAGAGACGT AACTCCTCGA GCTCTGTGAG CAGCCGCCTG 
AGTATCTACG ACAACGTGCC AGGCTCCATC CTCTATTCCA GCTCAGGTGA CCTGGCCGAT 
CTGGAGAACG AGGACATCTT CCCTGAGCTG GACGACATCC TCTACCATGT GAAGGGGATG 
CAGAGGATAG TCAACCAGTG GTCAGAGAAG TTTTCCGATG AGGGAGACTC CGACTCGGCC 
TTGGACTCGG TCTCACCATG CCCGTCCTCT CCCAAGCAGA TACACCTGGA TGTGGACAAT 
GACCGAGCCA CCCCCAGTGA CCTGGACAGC ACAGGCAACT CACTGAATGA GCCTGAGGAG 
CCCTCTGACA TCCCGGAAAG GAGGGATTCT GGGGTCGGGG CCTCCCTGAC CAGGTCCAAC 
AGGCACAGGC TGAGATGGCA CAGCTTCCAG AGCTCTCATC GGCCGAGCCT AAACTCCGTG 
TCACTGCAGA TTAACTGCCA GTCAGTGGCC CAGATGAACC TGCTGCAGAA GTACTCCCTC 
TTGAAGTTAA CCGCCCTGCT GGAGAAATAC ACGCCTTCTA ATAAGCATGG CTTTAGCTGG 
GCTGTGCCCA AGTTCATGAA AAGGATCAAG GTTCCAGACT ACAAGGACCG GAATGTATTC 
GGAGTCCCTC TGACAGTCAA CGTGCAGCGC ACAGGACAGC CCCTCCCCCA GAGCATCCAG 
CAGGCCATGC GCTACCTCCG CAACCATTGT TTGGATCAGG TTGGACTCTT CAGAAAATCG 
GGTGTCAAAT CCCGGATTCA GGCTCTGCGC CAGATGAATG AAAGCACCAT AGACTGTGTC 
AACTATGAGG GACAGTCTGC TTATGACGTG GCAGACATGT TGAAGCAGTA TTTTCGAGAT 
CTGCCTGAGC CACTGATGAC AAACAAACTC TCAGAAACCT TTCTGCAGAT ATACCAATAT 
GTGCCCAAGG ACCAGCGTCT CCAGGCGATC AAGGCGGCCA TCATGCTCCT GCCCGACGAG 
AACCGGGAGG TCCTACAGAC GCTCCTTTAC TTCCTGAGCG ATGTCACCGC AGCCGTCAAG 
GAGAACCAGA TGACCCCCAC CAACCTGGCC GTGTGCTTGG CGCCTTCTCT CTTCCACCTC 
AACACTCTGA AGAGAGAGAA CTCCTCCCCC AGGGTAATGC AAAGAAAACA GAGTTTGGGC 
AAACCAGATC AAAAAGATCT GAATGAGAAT CTCGCTGCCA CTCAAGGGCT AGCCCATATG 
ATTGCTGAGT GCAAGAAGCT TTTCCAGGTT CCTGAGGAAA TGAGCCGATG TCGAAATTCC 
TACACCGAAC AGGAGCTAAA GCCCCTTACC CTCGAAGCGT TAGGACGCCT TTGTAATGAT 
GACTCAGCCG ACTACCAACA CTTCCTCCAA GACTGTGTGG ACAGCCTGTT TAAAGAGGTC 
AAGGAGAAGT TCAAAGGCTG GGTCAGTTAC TCCACATCTG AGCAGGCCGA GCTCTCTTAT 
AAGAAGGTGA GTGAAGGACC ACCTCTGAGG CTTTGGCGGG CAACCATCGA AGTCCCTGCA 
ACACCCGAGG AAATCTTAAA GCGCCTCCTG AAAGAGCAGC ACCTCTGGGA TGTAGACTTG 
TTGGATTCAA AAGTGATTGA AATCCTGGAC AGCCAGACTG AAATTTACCA GTATGTCCAA 
AACAGTATGG CACCCCATCC TGCTCGGGAC TATGTCGTTT TGAGAACATG GAGGACTAAT 
TTACCCAAGG GAGCCTGTGC CCTTTTACTC ACCTCGGTGG ATCATGACCG GGCCCCTGTG 
GTGGGAGTGA GGGTCAATGT GCTCCTGGCC AGGTATCTGA TCGAACCCTG TGGGTCAGGG 
AAATCCAAAC TCACCTACAT GTGCAGAGCA GACTTAAGGG GCCACATGCC AGAGTGGTAC 
ACGAAATCTT TTGGACATTT GTGTGCAGCT GAAGTGGTAA AGATCCGAGA CTCCTTCAGT 
CATCAGAACA CTGAAACCAA AGACACCAAA TCTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095963.1
CDS	84..3422
Translation	MARPLRAPLRRSFSDHIRDSTARALDVIWKNTRDRRLAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	NM_001102493.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102493.1

ORF Insert Sequence:

ATGGCGCGGC CCCTGAGAGC CCCTCTCCGG AGGTCCTTCA GTGATCACAT CCGAGACTCC 
ACGGCCAGAG CCCTGGATGT CATTTGGAAA AACACCCGAG ACAGGCGGCT GGCAGAAATC 
GAAGCCAAAG AAGCTTGTGA TTGGCTCCGA GCCGCAGGAT TCCCCCAGTA TGCCCAGCTT 
TATGAAGATC TTCTGTTCCC CATTGATATT TCCTCAGTCA AGAGAGAGCA TGATTTTTTG 
GACAGAGATG CTATTGAGGC TTTATGCAGG CGTCTAAATA CTTTAAACAA ATGTGCAGTG 
ATGAAGCTGG AAATCAGTCC TCATCGGAAA AGAAGCGAAG ATTCCGACGA GGACGAGCCT 
TGTGCAATAA GTGGCAAATG GACTTTCCAG AGGGACAGCA AGAGGTGGTC CCGGCTTGAA 
GAGTTCGACG TCTTTTCTCC AAAACAGGAC CCCATCCCTG GGTCCCCAGA CGCTGTCCAC 
CTGAAGAGCG CCCCGAGCCA TGAAAACATG CAGACGGACC TCAGCGATCG CCAGGAGGTG 
GCTTCTGTCC ACAGCACCGG CAGCCTCACC ACCCACGCGC CCCAGCGCGG GGAGGCGGCC 
CCAGCCCGGA CTAACTCCGT CCTCAGCGTC TGCTCCTCAG GCACCTTTGT AGGCAATGAT 
GATTCCTTCT GCAGCCTGCC CTCTCCCAAG GAACTGTCCA GCTTCAGCTT CAGCATGAAG 
GGCCACGAGA AGGCTGCCAA GTCCAAGACG CACAGCCTGC TGAAGCGCAT GGAGAGCTTA 
AAGCTCAAGG GCTCCCACCA CAGCAAGCAC AAGGCGCCTT CCAAGCTGGG TCTGATCATC 
AGTGGGCCCA TCCTGCAGGA GGGGGTGGAC GAGGAGAAGC TGAAGCAGCT GAACTGCGTG 
GAGATCTCCG CCCTCAACGG CAACCACATC AACGTCCCCA TGGTACGGAA GAGGAGCATA 
TCCAGCTCCA CGCAGACCAG CAGCAGCAGC AGCCAGTCTG AGACCAGCAG CAACGTGAGC 
ACGCCCAGTC CCGTCACCAG GACCCGGAGC CTCAGCGCCT GCAACAAGCG GGGAGGCATG 
TACTTGGAGG GCTTCGATCC CTTCAATCAG TCCACCTTCA ACAACGTCAT GGAGCAGAAC 
TGTAAAAACC GGGAGAGCTA CCCAGAGGAC ACAGTGTTCT ACATCCCGGA AGATCACAAG 
CCCGGCACCT TCCCGAAAGC CCTTTCCAAT GGCAGTTTCT CTCCCTCAGG GAATAACAGC 
TCTGTGAACT GGAGGACCGG GAGCTTCCAC GGCCCCGGCC ACATCAGCCT GAGGAGAGAA 
AACAGCAGCC CCAAGGAACT TAAGAGACGT AACTCCTCGA GCTCTGTGAG CAGCCGCCTG 
AGTATCTACG ACAACGTGCC AGGCTCCATC CTCTATTCCA GCTCAGGTGA CCTGGCCGAT 
CTGGAGAACG AGGACATCTT CCCTGAGCTG GACGACATCC TCTACCATGT GAAGGGGATG 
CAGAGGATAG TCAACCAGTG GTCAGAGAAG TTTTCCGATG AGGGAGACTC CGACTCGGCC 
TTGGACTCGG TCTCACCATG CCCGTCCTCT CCCAAGCAGA TACACCTGGA TGTGGACAAT 
GACCGAGCCA CCCCCAGTGA CCTGGACAGC ACAGGCAACT CACTGAATGA GCCTGAGGAG 
CCCTCTGACA TCCCGGAAAG GAGGGATTCT GGGGTCGGGG CCTCCCTGAC CAGGTCCAAC 
AGGCACAGGC TGAGATGGCA CAGCTTCCAG AGCTCTCATC GGCCGAGCCT AAACTCCGTG 
TCACTGCAGA TTAACTGCCA GTCAGTGGCC CAGATGAACC TGCTGCAGAA GTACTCCCTC 
TTGAAGTTAA CCGCCCTGCT GGAGAAATAC ACGCCTTCTA ATAAGCATGG CTTTAGCTGG 
GCTGTGCCCA AGTTCATGAA AAGGATCAAG GTTCCAGACT ACAAGGACCG GAATGTATTC 
GGAGTCCCTC TGACAGTCAA CGTGCAGCGC ACAGGACAGC CCCTCCCCCA GAGCATCCAG 
CAGGCCATGC GCTACCTCCG CAACCATTGT TTGGATCAGG TTGGACTCTT CAGAAAATCG 
GGTGTCAAAT CCCGGATTCA GGCTCTGCGC CAGATGAATG AAAGCACCAT AGACTGTGTC 
AACTATGAGG GACAGTCTGC TTATGACGTG GCAGACATGT TGAAGCAGTA TTTTCGAGAT 
CTGCCTGAGC CACTGATGAC AAACAAACTC TCAGAAACCT TTCTGCAGAT ATACCAATAT 
GTGCCCAAGG ACCAGCGTCT CCAGGCGATC AAGGCGGCCA TCATGCTCCT GCCCGACGAG 
AACCGGGAGG TCCTACAGAC GCTCCTTTAC TTCCTGAGCG ATGTCACCGC AGCCGTCAAG 
GAGAACCAGA TGACCCCCAC CAACCTGGCC GTGTGCTTGG CGCCTTCTCT CTTCCACCTC 
AACACTCTGA AGAGAGAGAA CTCCTCCCCC AGGGTAATGC AAAGAAAACA GAGTTTGGGC 
AAACCAGATC AAAAAGATCT GAATGAGAAT CTCGCTGCCA CTCAAGGGCT AGCCCATATG 
ATTGCTGAGT GCAAGAAGCT TTTCCAGGTT CCTGAGGAAA TGAGCCGATG TCGAAATTCC 
TACACCGAAC AGGAGCTAAA GCCCCTTACC CTCGAAGCGT TAGGACGCCT TTGTAATGAT 
GACTCAGCCG ACTACCAACA CTTCCTCCAA GACTGTGTGG ACAGCCTGTT TAAAGAGGTC 
AAGGAGAAGT TCAAAGGCTG GGTCAGTTAC TCCACATCTG AGCAGGCCGA GCTCTCTTAT 
AAGAAGGTGA GTGAAGGACC ACCTCTGAGG CTTTGGCGGG CAACCATCGA AGTCCCTGCA 
ACACCCGAGG AAATCTTAAA GCGCCTCCTG AAAGAGCAGC ACCTCTGGGA TGTAGACTTG 
TTGGATTCAA AAGTGATTGA AATCCTGGAC AGCCAGACTG AAATTTACCA GTATGTCCAA 
AACAGTATGG CACCCCATCC TGCTCGGGAC TATGTCGTTT TGAGAACATG GAGGACTAAT 
TTACCCAAGG GAGCCTGTGC CCTTTTACTC ACCTCGGTGG ATCATGACCG GGCCCCTGTG 
GTGGGAGTGA GGGTCAATGT GCTCCTGGCC AGGTATCTGA TCGAACCCTG TGGGTCAGGG 
AAATCCAAAC TCACCTACAT GTGCAGAGCA GACTTAAGGG GCCACATGCC AGAGTGGTAC 
ACGAAATCTT TTGGACATTT GTGTGCAGCT GAAGTGGTAA AGATCCGAGA CTCCTTCAGT 
CATCAGAACA CTGAAACCAA AGACACCAAA TCTAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( XM_024986238.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842006.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X2, mRNA.

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CloneID	OBa231340
Clone ID Related Accession (Same CDS sequence)	XM_024986238.1 , XM_024986239.1 , XM_024986237.1
Accession Version	XM_024986238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842006.1
CDS	438..5009
Translation	MSVAIRKRSWEEHVTQWMGQPFSSDDCNTACHHGLVADSLQASMEKDATLNVDRKERCVSLPDCCHGSDLREIPEGPMGHISKEVDEDDSHEGEDQFLSLEASTETLVHVSDEDTDSDLYLTDDKQILTTQGPETSDQHSVKGAGSLVKTLPIVESNQDSYDSWGMAGGVDLGLVEEKGDKKVVDMVSKSLELCKEISLSEIKDRSKMNACSSLNVKDIVPEKQLVNSAVIAQHRRKPDFPKDEHESNTCSAVQDKLLAIPRMDRGLPLLKTDPGSCLLQPPSCPSGMSAENGLETSGFSEHQNKSLPKFNSGDGMQCLHFKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSSSSSPSSTPTSLRRQVPDLESGPESGTDTTSINQTQVNLSSNTESTDPQSSTPVANSGTKPKSMAIPGLSEKEKAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	XM_024986238.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986238.1

ORF Insert Sequence:

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( XM_024986239.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842007.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X3, mRNA.

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CloneID	OBa231340
Clone ID Related Accession (Same CDS sequence)	XM_024986238.1 , XM_024986239.1 , XM_024986237.1
Accession Version	XM_024986239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842007.1
CDS	233..4804
Translation	MSVAIRKRSWEEHVTQWMGQPFSSDDCNTACHHGLVADSLQASMEKDATLNVDRKERCVSLPDCCHGSDLREIPEGPMGHISKEVDEDDSHEGEDQFLSLEASTETLVHVSDEDTDSDLYLTDDKQILTTQGPETSDQHSVKGAGSLVKTLPIVESNQDSYDSWGMAGGVDLGLVEEKGDKKVVDMVSKSLELCKEISLSEIKDRSKMNACSSLNVKDIVPEKQLVNSAVIAQHRRKPDFPKDEHESNTCSAVQDKLLAIPRMDRGLPLLKTDPGSCLLQPPSCPSGMSAENGLETSGFSEHQNKSLPKFNSGDGMQCLHFKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSSSSSPSSTPTSLRRQVPDLESGPESGTDTTSINQTQVNLSSNTESTDPQSSTPVANSGTKPKSMAIPGLSEKEKAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	XM_024986239.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986239.1

ORF Insert Sequence:

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLC1 ( XM_024986237.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842005.1 Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X1, mRNA.

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CloneID	OBa231340
Clone ID Related Accession (Same CDS sequence)	XM_024986238.1 , XM_024986239.1 , XM_024986237.1
Accession Version	XM_024986237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842005.1
CDS	460..5031
Translation	MSVAIRKRSWEEHVTQWMGQPFSSDDCNTACHHGLVADSLQASMEKDATLNVDRKERCVSLPDCCHGSDLREIPEGPMGHISKEVDEDDSHEGEDQFLSLEASTETLVHVSDEDTDSDLYLTDDKQILTTQGPETSDQHSVKGAGSLVKTLPIVESNQDSYDSWGMAGGVDLGLVEEKGDKKVVDMVSKSLELCKEISLSEIKDRSKMNACSSLNVKDIVPEKQLVNSAVIAQHRRKPDFPKDEHESNTCSAVQDKLLAIPRMDRGLPLLKTDPGSCLLQPPSCPSGMSAENGLETSGFSEHQNKSLPKFNSGDGMQCLHFKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSSSSSPSSTPTSLRRQVPDLESGPESGTDTTSINQTQVNLSSNTESTDPQSSTPVANSGTKPKSMAIPGLSEKEKAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDAVHLKSAPSHENMQTDLSDRQEVASVHSTGSLTTHAPQRGEAAPARTNSVLSVCSSGTFVGNDDSFCSLPSPKELSSFSFSMKGHEKAAKSKTHSLLKRMESLKLKGSHHSKHKAPSKLGLIISGPILQEGVDEEKLKQLNCVEISALNGNHINVPMVRKRSISSSTQTSSSSSQSETSSNVSTPSPVTRTRSLSACNKRGGMYLEGFDPFNQSTFNNVMEQNCKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFSPSGNNSSVNWRTGSFHGPGHISLRRENSSPKELKRRNSSSSVSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRATPSDLDSTGNSLNEPEEPSDIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESTIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGRLCNDDSADYQHFLQDCVDSLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRATIEVPATPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLARYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSHQNTETKDTKSR

Target ORF information:

RefSeq Version	XM_024986237.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DLC1 Rho GTPase activating protein (DLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986237.1

ORF Insert Sequence:

ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATGGGACAG 
CCTTTTAGTT CTGATGATTG TAACACAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG 
CAGGCAAGTA TGGAAAAAGA TGCAACTCTA AATGTGGACC GCAAAGAGAG GTGTGTTTCA 
CTCCCAGACT GCTGTCATGG CTCAGACCTG AGAGAGATTC CTGAGGGGCC AATGGGTCAC 
ATTTCAAAGG AGGTGGATGA AGATGACAGC CATGAAGGTG AAGATCAGTT CCTTTCTCTG 
GAGGCCAGCA CAGAAACACT TGTGCATGTT TCTGATGAGG ACACCGATTC GGATCTGTAC 
CTCACAGATG ATAAACAGAT TCTAACTACT CAAGGGCCTG AAACATCAGA CCAACATAGC 
GTCAAAGGAG CAGGCTCCTT AGTAAAGACG CTGCCCATTG TGGAAAGTAA CCAAGATTCT 
TATGACTCCT GGGGAATGGC TGGTGGAGTT GATTTAGGGC TGGTTGAGGA AAAGGGGGAC 
AAGAAAGTTG TTGACATGGT GAGTAAAAGC CTGGAGCTTT GTAAGGAGAT AAGCTTAAGT 
GAAATAAAAG ATCGGTCCAA AATGAATGCT TGCAGTTCTT TGAATGTGAA AGATATTGTG 
CCAGAAAAGC AGTTGGTTAA TTCTGCTGTA ATTGCTCAAC ATCGAAGGAA ACCCGACTTC 
CCTAAAGATG AACATGAAAG CAACACCTGC AGTGCAGTAC AAGATAAGTT ATTGGCTATT 
CCTCGTATGG ACAGAGGACT GCCATTATTA AAAACAGATC CTGGAAGCTG CCTTCTGCAG 
CCTCCTTCCT GCCCTAGTGG AATGTCAGCT GAAAATGGCC TGGAGACGAG TGGTTTCTCA 
GAACATCAAA ACAAAAGTCT TCCAAAGTTC AACTCAGGAG ATGGCATGCA GTGTTTACAC 
TTCAAGGAGA CTCTGGCCAC CCAGGAACCC ACAGATAACC AAGTCAGACT TCGTAAAAGA 
AAGGAAATAA GAGAAGATCG GGATAGGGCA CGACTAGACT CGATGGTGCT GCTGATCATG 
AAACTGGACC AGCTCGATCA GGACATTGAG AATGCCCTCA GCTCCAGCTC CTCTCCGTCC 
AGCACACCAA CCAGCCTGCG GCGGCAAGTC CCTGATCTGG AATCAGGACC TGAGAGTGGA 
ACAGATACCA CTTCGATAAA TCAGACACAA GTAAATTTGT CTTCTAACAC TGAGTCCACT 
GACCCACAGT CCTCCACCCC AGTGGCCAAT TCTGGAACCA AACCCAAGTC TATGGCGATT 
CCAGGTCTCT CAGAGAAGGA AAAGGCTGAA ATCGAAGCCA AAGAAGCTTG TGATTGGCTC 
CGAGCCGCAG GATTCCCCCA GTATGCCCAG CTTTATGAAG ATCTTCTGTT CCCCATTGAT 
ATTTCCTCAG TCAAGAGAGA GCATGATTTT TTGGACAGAG ATGCTATTGA GGCTTTATGC 
AGGCGTCTAA ATACTTTAAA CAAATGTGCG GTGATGAAGC TGGAAATCAG TCCTCATCGG 
AAAAGAAGCG AAGATTCCGA CGAGGACGAG CCTTGTGCAA TAAGTGGCAA ATGGACTTTC 
CAGAGGGACA GCAAGAGGTG GTCCCGGCTT GAAGAGTTCG ACGTCTTTTC TCCAAAACAG 
GACCCCATCC CTGGGTCCCC AGACGCTGTC CACCTGAAGA GCGCCCCGAG CCATGAAAAC 
ATGCAGACGG ACCTCAGCGA TCGCCAGGAG GTGGCTTCTG TCCACAGCAC CGGCAGCCTC 
ACCACCCACG CGCCCCAGCG CGGGGAGGCG GCCCCAGCCC GGACTAACTC CGTCCTCAGC 
GTCTGCTCCT CAGGCACCTT TGTAGGCAAT GATGATTCCT TCTGCAGCCT GCCCTCTCCC 
AAGGAACTGT CCAGCTTCAG CTTCAGCATG AAGGGCCACG AGAAGGCTGC CAAGTCCAAG 
ACGCACAGCC TGCTGAAGCG CATGGAGAGC TTAAAGCTCA AGGGCTCCCA CCACAGCAAG 
CACAAGGCGC CTTCCAAGCT GGGTCTGATC ATCAGTGGGC CCATCCTGCA GGAGGGGGTG 
GACGAGGAGA AGCTGAAGCA GCTGAACTGC GTGGAGATCT CCGCCCTCAA CGGCAACCAC 
ATCAACGTCC CCATGGTACG GAAGAGGAGC ATATCCAGCT CCACGCAGAC CAGCAGCAGC 
AGCAGCCAGT CTGAGACCAG CAGCAACGTG AGCACGCCCA GTCCCGTCAC CAGGACCCGG 
AGCCTCAGCG CCTGCAACAA GCGGGGAGGC ATGTACTTGG AGGGCTTCGA TCCCTTCAAT 
CAGTCCACCT TCAACAACGT CATGGAGCAG AACTGTAAAA ACCGGGAGAG CTACCCAGAG 
GACACAGTGT TCTACATCCC GGAAGATCAC AAGCCCGGCA CCTTCCCGAA AGCCCTTTCC 
AATGGCAGTT TCTCTCCCTC AGGGAATAAC AGCTCTGTGA ACTGGAGGAC CGGGAGCTTC 
CACGGCCCCG GCCACATCAG CCTGAGGAGA GAAAACAGCA GCCCCAAGGA ACTTAAGAGA 
CGTAACTCCT CGAGCTCTGT GAGCAGCCGC CTGAGTATCT ACGACAACGT GCCAGGCTCC 
ATCCTCTATT CCAGCTCAGG TGACCTGGCC GATCTGGAGA ACGAGGACAT CTTCCCTGAG 
CTGGACGACA TCCTCTACCA TGTGAAGGGG ATGCAGAGGA TAGTCAACCA GTGGTCAGAG 
AAGTTTTCCG ATGAGGGAGA CTCCGACTCG GCCTTGGACT CGGTCTCACC ATGCCCGTCC 
TCTCCCAAGC AGATACACCT GGATGTGGAC AATGACCGAG CCACCCCCAG TGACCTGGAC 
AGCACAGGCA ACTCACTGAA TGAGCCTGAG GAGCCCTCTG ACATCCCGGA AAGGAGGGAT 
TCTGGGGTCG GGGCCTCCCT GACCAGGTCC AACAGGCACA GGCTGAGATG GCACAGCTTC 
CAGAGCTCTC ATCGGCCGAG CCTAAACTCC GTGTCACTGC AGATTAACTG CCAGTCAGTG 
GCCCAGATGA ACCTGCTGCA GAAGTACTCC CTCTTGAAGT TAACCGCCCT GCTGGAGAAA 
TACACGCCTT CTAATAAGCA TGGCTTTAGC TGGGCTGTGC CCAAGTTCAT GAAAAGGATC 
AAGGTTCCAG ACTACAAGGA CCGGAATGTA TTCGGAGTCC CTCTGACAGT CAACGTGCAG 
CGCACAGGAC AGCCCCTCCC CCAGAGCATC CAGCAGGCCA TGCGCTACCT CCGCAACCAT 
TGTTTGGATC AGGTTGGACT CTTCAGAAAA TCGGGTGTCA AATCCCGGAT TCAGGCTCTG 
CGCCAGATGA ATGAAAGCAC CATAGACTGT GTCAACTATG AGGGACAGTC TGCTTATGAC 
GTGGCAGACA TGTTGAAGCA GTATTTTCGA GATCTGCCTG AGCCACTGAT GACAAACAAA 
CTCTCAGAAA CCTTTCTGCA GATATACCAA TATGTGCCCA AGGACCAGCG TCTCCAGGCG 
ATCAAGGCGG CCATCATGCT CCTGCCCGAC GAGAACCGGG AGGTCCTACA GACGCTCCTT 
TACTTCCTGA GCGATGTCAC CGCAGCCGTC AAGGAGAACC AGATGACCCC CACCAACCTG 
GCCGTGTGCT TGGCGCCTTC TCTCTTCCAC CTCAACACTC TGAAGAGAGA GAACTCCTCC 
CCCAGGGTAA TGCAAAGAAA ACAGAGTTTG GGCAAACCAG ATCAAAAAGA TCTGAATGAG 
AATCTCGCTG CCACTCAAGG GCTAGCCCAT ATGATTGCTG AGTGCAAGAA GCTTTTCCAG 
GTTCCTGAGG AAATGAGCCG ATGTCGAAAT TCCTACACCG AACAGGAGCT AAAGCCCCTT 
ACCCTCGAAG CGTTAGGACG CCTTTGTAAT GATGACTCAG CCGACTACCA ACACTTCCTC 
CAAGACTGTG TGGACAGCCT GTTTAAAGAG GTCAAGGAGA AGTTCAAAGG CTGGGTCAGT 
TACTCCACAT CTGAGCAGGC CGAGCTCTCT TATAAGAAGG TGAGTGAAGG ACCACCTCTG 
AGGCTTTGGC GGGCAACCAT CGAAGTCCCT GCAACACCCG AGGAAATCTT AAAGCGCCTC 
CTGAAAGAGC AGCACCTCTG GGATGTAGAC TTGTTGGATT CAAAAGTGAT TGAAATCCTG 
GACAGCCAGA CTGAAATTTA CCAGTATGTC CAAAACAGTA TGGCACCCCA TCCTGCTCGG 
GACTATGTCG TTTTGAGAAC ATGGAGGACT AATTTACCCA AGGGAGCCTG TGCCCTTTTA 
CTCACCTCGG TGGATCATGA CCGGGCCCCT GTGGTGGGAG TGAGGGTCAA TGTGCTCCTG 
GCCAGGTATC TGATCGAACC CTGTGGGTCA GGGAAATCCA AACTCACCTA CATGTGCAGA 
GCAGACTTAA GGGGCCACAT GCCAGAGTGG TACACGAAAT CTTTTGGACA TTTGTGTGCA 
GCTGAAGTGG TAAAGATCCG AGACTCCTTC AGTCATCAGA ACACTGAAAC CAAAGACACC 
AAATCTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DLC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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