NOSIP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOSIP
Entrez Gene ID	511234
Full Name	nitric oxide synthase interacting protein
General protein information	Preferred Names nitric oxide synthase interacting protein Names nitric oxide synthase-interacting protein E3 ubiquitin-protein ligase NOSIP RING-type E3 ubiquitin transferase NOSIP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010815027.1	XP_010813329.1	nitric oxide synthase-interacting protein isoform X1
XM_024978131.1	XP_024833899.1	nitric oxide synthase-interacting protein isoform X1
NM_001075502.1	NP_001068970.1	nitric oxide synthase-interacting protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	NOSIP	NP_057037.1
Bos taurus (cattle)	NOSIP	NP_001068970.1
Arabidopsis thaliana (thale cress)	AT1G61620	NP_564781.1
Canis lupus familiaris (dog)	NOSIP	XP_541493.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005654	XP_315673.3
Caenorhabditis elegans (roundworm)	R05G6.4	NP_501214.1
Oryza sativa (rice)	Os03g0279900	NP_001049735.1
Pan troglodytes (chimpanzee)	NOSIP	XP_512823.2
	NOSIP	XP_001115348.1
Rattus norvegicus (Norway rat)	Nosip	NP_001099730.1
Xenopus tropicalis (tropical clawed frog)	nosip	NP_989029.1
Mus musculus (house mouse)	Nosip	NP_079809.1
Danio rerio (zebrafish)	nosip	NP_001007435.1
Drosophila melanogaster (fruit fly)	CG6179	NP_573288.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Direct interaction of the cell division cycle 37 homolog inhibits endothelial nitric oxide synthase activity.
Harris MB, Bartoli M, Sood SG, Matts RL, Venema RC
Circulation research98(3)335-41(2006 Feb)

Endothelial dihydrofolate reductase: critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase.
Chalupsky K, Cai H
Proceedings of the National Academy of Sciences of the United States of America102(25)9056-61(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc37 has a direct regulatory interaction with endothelial nitric oxide synthase (eNOS) and may play an important role in mediating the eNOS protein complex formation.
Title: Direct interaction of the cell division cycle 37 homolog inhibits endothelial nitric oxide synthase activity.

Hydrogen peroxide down regulates endothelial dihydrofolate reductase expression in response to angiotensin Ii and underlies endothelial nitric oxide synthase dysfunction.
Title: Endothelial dihydrofolate reductase: critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase.

The following NOSIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOSIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42257	NM_001075502.1 Latest version!	Bos taurus nitric oxide synthase interacting protein (NOSIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa47960	XM_024978131.1 Latest version!	Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa47960	XM_010815027.1 Latest version!	Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOSIP ( NM_001075502.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068970.1 Bos taurus nitric oxide synthase interacting protein (NOSIP), mRNA.

Price & Availability↑

CloneID	OBa42257
Clone ID Related Accession (Same CDS sequence)	NM_001075502.1
Accession Version	NM_001075502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	nitric oxide synthase-interacting protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104600.1. On or before Oct 4, 2006 this sequence version replaced XM_882365.1, XM_588530.2, XM_869139.1. ##Evidence-Data-START## Transcript exon combination :: BC104600.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGACGCGGC ACGGCAAGAA CTGCACAGCA GGGGCCGTCT ACACCTACCA TGAGAAAAAG 
AAGGACACAG CGGCCTCAGG CTATGGCACC CAGAACATTC GACTGAGCCG GGATGCCGTC 
AAGGACTTCG ACTGCTGCTG CCTCTCGCTG CAGCCCTGCC ACGACCCTGT CGTCACCCCA 
GATGGCTACC TGTATGAGCG GGAAGCAATC CTCGAGTACA TTTTGCACCA GAAGAAGGAA 
ATTGCCCGGC AGATGAAGGC CTACGAGAAG CAGCGGGGCG CCCGGCGAGA GGAGCAGAAG 
GAGCTGCAGC GGGCAGCGGC GCAGGACCAC GTGCGGGGCT TCCTGGAGAA GGAGGCAGCC 
ATCGTGAGCC GGCCCCTCAA CCCCTTCACG CCCAAGGCCG CCTCGGCAGG GAACGGCCCA 
GACGATGCCC AACCCGGGTC CAGTGCGGGC CCTGCAGGCA AGGACAAGGA CAAAGCGCTG 
CCCAGCTTCT GGATCCCGTC GCTGACCCCC GAGGCGAAGG CCACGAAGCT GGAGAAGCCG 
TCGCGCATCG TGACCTGCCC CATGTCCGGG AAGCCGCTGC GCATGTCCGA CTTGACGCCG 
GTGCGCTTCA CGCCGCTGGA CAGCTCCGTG GACCGCGTGG GGCTCATCAC GCGCAGCGAG 
CGCTACGTGT GCGCCGTGAC CCGCGACAGC CTGAGCAACG CCACGCCGTG CGCCGTGCTG 
CGGCCCTCTG GGGCTGTGGT CACCCTGGAG TGCGTGGAGA AGCTGATTCG CAAGGACATG 
GTGGACCCGG TGACCGGGGA GAAGCTCACG GACCGTGACA TCATCGTGCT GCAGCGGGGC 
GGCACCGGCT TCGCGGGCTC CGGAGTGAAG CTGCAGGCGG AGAAGTCCCG GCCGGTGATG 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068970.1
CDS	183..1091
Translation	MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGARREEQKELQRAAAQDHVRGFLEKEAAIVSRPLNPFTPKAASAGNGPDDAQPGSSAGPAGKDKDKALPSFWIPSLTPEAKATKLEKPSRIVTCPMSGKPLRMSDLTPVRFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGEKLTDRDIIVLQRGGTGFAGSGVKLQAEKSRPVMQA

Target ORF information:

RefSeq Version	NM_001075502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase interacting protein (NOSIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075502.1

ORF Insert Sequence:

ATGACGCGGC ACGGCAAGAA CTGCACAGCA GGGGCCGTCT ACACCTACCA TGAGAAAAAG 
AAGGACACAG CGGCCTCAGG CTATGGCACC CAGAACATTC GACTGAGCCG GGATGCCGTC 
AAGGACTTCG ACTGCTGCTG CCTCTCGCTG CAGCCCTGCC ACGACCCTGT CGTCACCCCA 
GATGGCTACC TGTATGAGCG GGAAGCAATC CTCGAGTACA TTTTGCACCA GAAGAAGGAA 
ATTGCCCGGC AGATGAAGGC CTACGAGAAG CAGCGGGGCG CCCGGCGAGA GGAGCAGAAG 
GAGCTGCAGC GGGCAGCGGC GCAGGACCAC GTGCGGGGCT TCCTGGAGAA GGAGGCAGCC 
ATCGTGAGCC GGCCCCTCAA CCCCTTCACG CCCAAGGCCG CCTCGGCAGG GAACGGCCCA 
GACGATGCCC AACCCGGGTC CAGTGCGGGC CCTGCAGGCA AGGACAAGGA CAAAGCGCTG 
CCCAGCTTCT GGATCCCGTC GCTGACCCCC GAGGCGAAGG CCACGAAGCT GGAGAAGCCG 
TCGCGCATCG TGACCTGCCC CATGTCCGGG AAGCCGCTGC GCATGTCCGA CTTGACGCCG 
GTGCGCTTCA CGCCGCTGGA CAGCTCCGTG GACCGCGTGG GGCTCATCAC GCGCAGCGAG 
CGCTACGTGT GCGCCGTGAC CCGCGACAGC CTGAGCAACG CCACGCCGTG CGCCGTGCTG 
CGGCCCTCTG GGGCTGTGGT CACCCTGGAG TGCGTGGAGA AGCTGATTCG CAAGGACATG 
GTGGACCCGG TGACCGGGGA GAAGCTCACG GACCGTGACA TCATCGTGCT GCAGCGGGGC 
GGCACCGGCT TCGCGGGCTC CGGAGTGAAG CTGCAGGCGG AGAAGTCCCG GCCGGTGATG 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOSIP ( XM_010815027.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813329.1 Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47960
Clone ID Related Accession (Same CDS sequence)	XM_010815027.1 , XM_024978131.1
Accession Version	XM_010815027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nitric oxide synthase-interacting protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAC TGAGGCCCAG AGAGATGCGG GCGCTTGAGC TGGGTCCCAG AGCCAGAAGG 
CCCGAGGGGA GTCAGCCACG AGCCTCTTCC CTCAGCTGGC CCTCGGGCTG CCTCAGAGCC 
TGTCACTTAC CCTCTGGGGA TGAACACCTC CTGTGGATGT GCCCCGCCCC CAACACACAG 
ACCCCGGGCA CAAGCGCCCC CCACCTCTGT TGTGTCCTCG CTGTGCCTTT CTCTCCGGAT 
TTGCACAGGG GCACGTCCCC TGTCTCTCCG TCCGTCTGTG TGTTCCTGTC CCCAGCGGCC 
TCAGGCTATG GCACCCAGAA CATTCGACTG AGCCGGGATG CCGTCAAGGA CTTCGACTGC 
TGCTGCCTCT CGCTGCAGCC CTGCCACGAC CCTGTCGTCA CCCCAGATGG CTACCTGTAT 
GAGCGGGAAG CAATCCTCGA GTACATTTTG CACCAGAAGA AGGAAATTGC CCGGCAGATG 
AAGGCCTACG AGAAGCAGCG GGGCGCCCGG CGAGAGGAGC AGAAGGAGCT GCAGCGGGCA 
GCGGCGCAGG ACCACGTGCG GGGCTTCCTG GAGAAGGAGG CAGCCATCGT GAGCCGGCCC 
CTCAACCCCT TCACCCCCAA GGCCGCCTCG GCAGGGAACG GCCCAGACGA TGCCCAACCC 
GGGTCCAGTG CGGGCCCTGC AGGCAAGGAC AAGGACAAAG CGCTGCCCAG CTTCTGGATC 
CCGTCGCTGA CCCCCGAGGC GAAGGCCACG AAGCTGGAGA AGCCGTCGCG CATCGTGACC 
TGCCCCATGT CCGGGAAGCC GCTGCGCATG TCCGACTTGA CGCCGGTGCG CTTCACGCCG 
CTGGACAGCT CCGTGGACCG CGTGGGGCTC ATCACGCGCA GCGAGCGCTA CGTGTGCGCC 
GTGACCCGCG ACAGCCTGAG CAACGCCACG CCGTGCGCCG TGCTGCGGCC CTCTGGGGCT 
GTGGTCACCC TGGAGTGCGT GGAGAAGCTG ATTCGCAAGG ACATGGTGGA CCCGGTGACC 
GGGGAGAAGC TCACGGACCG TGACATCATC GTGCTGCAGC GGGGCGGCAC CGGCTTCGCG 
GGCTCCGGAG TGAAGCTGCA GGCGGAGAAG TCCCGGCCGG TGATGCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813329.1
CDS	261..1394
Translation	MGKLRPREMRALELGPRARRPEGSQPRASSLSWPSGCLRACHLPSGDEHLLWMCPAPNTQTPGTSAPHLCCVLAVPFSPDLHRGTSPVSPSVCVFLSPAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGARREEQKELQRAAAQDHVRGFLEKEAAIVSRPLNPFTPKAASAGNGPDDAQPGSSAGPAGKDKDKALPSFWIPSLTPEAKATKLEKPSRIVTCPMSGKPLRMSDLTPVRFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGEKLTDRDIIVLQRGGTGFAGSGVKLQAEKSRPVMQA

Target ORF information:

RefSeq Version	XM_010815027.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815027.1

ORF Insert Sequence:

ATGGGGAAAC TGAGGCCCAG AGAGATGCGG GCGCTTGAGC TGGGTCCCAG AGCCAGAAGG 
CCCGAGGGGA GTCAGCCACG AGCCTCTTCC CTCAGCTGGC CCTCGGGCTG CCTCAGAGCC 
TGTCACTTAC CCTCTGGGGA TGAACACCTC CTGTGGATGT GCCCCGCCCC CAACACACAG 
ACCCCGGGCA CAAGCGCCCC CCACCTCTGT TGTGTCCTCG CTGTGCCTTT CTCTCCGGAT 
TTGCACAGGG GCACGTCCCC TGTCTCTCCG TCCGTCTGTG TGTTCCTGTC CCCAGCGGCC 
TCAGGCTATG GCACCCAGAA CATTCGACTG AGCCGGGATG CCGTCAAGGA CTTCGACTGC 
TGCTGCCTCT CGCTGCAGCC CTGCCACGAC CCTGTCGTCA CCCCAGATGG CTACCTGTAT 
GAGCGGGAAG CAATCCTCGA GTACATTTTG CACCAGAAGA AGGAAATTGC CCGGCAGATG 
AAGGCCTACG AGAAGCAGCG GGGCGCCCGG CGAGAGGAGC AGAAGGAGCT GCAGCGGGCA 
GCGGCGCAGG ACCACGTGCG GGGCTTCCTG GAGAAGGAGG CAGCCATCGT GAGCCGGCCC 
CTCAACCCCT TCACCCCCAA GGCCGCCTCG GCAGGGAACG GCCCAGACGA TGCCCAACCC 
GGGTCCAGTG CGGGCCCTGC AGGCAAGGAC AAGGACAAAG CGCTGCCCAG CTTCTGGATC 
CCGTCGCTGA CCCCCGAGGC GAAGGCCACG AAGCTGGAGA AGCCGTCGCG CATCGTGACC 
TGCCCCATGT CCGGGAAGCC GCTGCGCATG TCCGACTTGA CGCCGGTGCG CTTCACGCCG 
CTGGACAGCT CCGTGGACCG CGTGGGGCTC ATCACGCGCA GCGAGCGCTA CGTGTGCGCC 
GTGACCCGCG ACAGCCTGAG CAACGCCACG CCGTGCGCCG TGCTGCGGCC CTCTGGGGCT 
GTGGTCACCC TGGAGTGCGT GGAGAAGCTG ATTCGCAAGG ACATGGTGGA CCCGGTGACC 
GGGGAGAAGC TCACGGACCG TGACATCATC GTGCTGCAGC GGGGCGGCAC CGGCTTCGCG 
GGCTCCGGAG TGAAGCTGCA GGCGGAGAAG TCCCGGCCGG TGATGCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOSIP ( XM_024978131.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833899.1 Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47960
Clone ID Related Accession (Same CDS sequence)	XM_010815027.1 , XM_024978131.1
Accession Version	XM_024978131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nitric oxide synthase-interacting protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAC TGAGGCCCAG AGAGATGCGG GCGCTTGAGC TGGGTCCCAG AGCCAGAAGG 
CCCGAGGGGA GTCAGCCACG AGCCTCTTCC CTCAGCTGGC CCTCGGGCTG CCTCAGAGCC 
TGTCACTTAC CCTCTGGGGA TGAACACCTC CTGTGGATGT GCCCCGCCCC CAACACACAG 
ACCCCGGGCA CAAGCGCCCC CCACCTCTGT TGTGTCCTCG CTGTGCCTTT CTCTCCGGAT 
TTGCACAGGG GCACGTCCCC TGTCTCTCCG TCCGTCTGTG TGTTCCTGTC CCCAGCGGCC 
TCAGGCTATG GCACCCAGAA CATTCGACTG AGCCGGGATG CCGTCAAGGA CTTCGACTGC 
TGCTGCCTCT CGCTGCAGCC CTGCCACGAC CCTGTCGTCA CCCCAGATGG CTACCTGTAT 
GAGCGGGAAG CAATCCTCGA GTACATTTTG CACCAGAAGA AGGAAATTGC CCGGCAGATG 
AAGGCCTACG AGAAGCAGCG GGGCGCCCGG CGAGAGGAGC AGAAGGAGCT GCAGCGGGCA 
GCGGCGCAGG ACCACGTGCG GGGCTTCCTG GAGAAGGAGG CAGCCATCGT GAGCCGGCCC 
CTCAACCCCT TCACCCCCAA GGCCGCCTCG GCAGGGAACG GCCCAGACGA TGCCCAACCC 
GGGTCCAGTG CGGGCCCTGC AGGCAAGGAC AAGGACAAAG CGCTGCCCAG CTTCTGGATC 
CCGTCGCTGA CCCCCGAGGC GAAGGCCACG AAGCTGGAGA AGCCGTCGCG CATCGTGACC 
TGCCCCATGT CCGGGAAGCC GCTGCGCATG TCCGACTTGA CGCCGGTGCG CTTCACGCCG 
CTGGACAGCT CCGTGGACCG CGTGGGGCTC ATCACGCGCA GCGAGCGCTA CGTGTGCGCC 
GTGACCCGCG ACAGCCTGAG CAACGCCACG CCGTGCGCCG TGCTGCGGCC CTCTGGGGCT 
GTGGTCACCC TGGAGTGCGT GGAGAAGCTG ATTCGCAAGG ACATGGTGGA CCCGGTGACC 
GGGGAGAAGC TCACGGACCG TGACATCATC GTGCTGCAGC GGGGCGGCAC CGGCTTCGCG 
GGCTCCGGAG TGAAGCTGCA GGCGGAGAAG TCCCGGCCGG TGATGCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833899.1
CDS	564..1697
Translation	MGKLRPREMRALELGPRARRPEGSQPRASSLSWPSGCLRACHLPSGDEHLLWMCPAPNTQTPGTSAPHLCCVLAVPFSPDLHRGTSPVSPSVCVFLSPAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGARREEQKELQRAAAQDHVRGFLEKEAAIVSRPLNPFTPKAASAGNGPDDAQPGSSAGPAGKDKDKALPSFWIPSLTPEAKATKLEKPSRIVTCPMSGKPLRMSDLTPVRFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGEKLTDRDIIVLQRGGTGFAGSGVKLQAEKSRPVMQA

Target ORF information:

RefSeq Version	XM_024978131.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase interacting protein (NOSIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978131.1

ORF Insert Sequence:

ATGGGGAAAC TGAGGCCCAG AGAGATGCGG GCGCTTGAGC TGGGTCCCAG AGCCAGAAGG 
CCCGAGGGGA GTCAGCCACG AGCCTCTTCC CTCAGCTGGC CCTCGGGCTG CCTCAGAGCC 
TGTCACTTAC CCTCTGGGGA TGAACACCTC CTGTGGATGT GCCCCGCCCC CAACACACAG 
ACCCCGGGCA CAAGCGCCCC CCACCTCTGT TGTGTCCTCG CTGTGCCTTT CTCTCCGGAT 
TTGCACAGGG GCACGTCCCC TGTCTCTCCG TCCGTCTGTG TGTTCCTGTC CCCAGCGGCC 
TCAGGCTATG GCACCCAGAA CATTCGACTG AGCCGGGATG CCGTCAAGGA CTTCGACTGC 
TGCTGCCTCT CGCTGCAGCC CTGCCACGAC CCTGTCGTCA CCCCAGATGG CTACCTGTAT 
GAGCGGGAAG CAATCCTCGA GTACATTTTG CACCAGAAGA AGGAAATTGC CCGGCAGATG 
AAGGCCTACG AGAAGCAGCG GGGCGCCCGG CGAGAGGAGC AGAAGGAGCT GCAGCGGGCA 
GCGGCGCAGG ACCACGTGCG GGGCTTCCTG GAGAAGGAGG CAGCCATCGT GAGCCGGCCC 
CTCAACCCCT TCACCCCCAA GGCCGCCTCG GCAGGGAACG GCCCAGACGA TGCCCAACCC 
GGGTCCAGTG CGGGCCCTGC AGGCAAGGAC AAGGACAAAG CGCTGCCCAG CTTCTGGATC 
CCGTCGCTGA CCCCCGAGGC GAAGGCCACG AAGCTGGAGA AGCCGTCGCG CATCGTGACC 
TGCCCCATGT CCGGGAAGCC GCTGCGCATG TCCGACTTGA CGCCGGTGCG CTTCACGCCG 
CTGGACAGCT CCGTGGACCG CGTGGGGCTC ATCACGCGCA GCGAGCGCTA CGTGTGCGCC 
GTGACCCGCG ACAGCCTGAG CAACGCCACG CCGTGCGCCG TGCTGCGGCC CTCTGGGGCT 
GTGGTCACCC TGGAGTGCGT GGAGAAGCTG ATTCGCAAGG ACATGGTGGA CCCGGTGACC 
GGGGAGAAGC TCACGGACCG TGACATCATC GTGCTGCAGC GGGGCGGCAC CGGCTTCGCG 
GGCTCCGGAG TGAAGCTGCA GGCGGAGAAG TCCCGGCCGG TGATGCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Direct interaction of the cell division cycle 37 homolog inhibits endothelial nitric oxide synthase activity.
Circulation research98(3)335-41(2006 Feb)
Harris MB,Bartoli M,Sood SG,Matts RL,Venema RC

Endothelial dihydrofolate reductase: critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase.
Proceedings of the National Academy of Sciences of the United States of America102(25)9056-61(2005 Jun)
Chalupsky K,Cai H