AARS cDNA ORF clone, Bos taurus(cattle)

The following AARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa28249 NM_001101075.2
Latest version!
Bos taurus alanyl-tRNA synthetase 1 (AARS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_005218342.4
Latest version!
Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_005218341.2
Latest version!
Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_015475667.2
Latest version!
Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_010814304.3
Latest version!
Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_005218343.3
Latest version!
Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa28249 XM_005218342.3 Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_010814304.2 Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_015475667.1 Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28249 XM_005218343.2 Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version NM_001101075.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC148083.1. On Aug 31, 2012 this sequence version replaced NM_001101075.1. ##Evidence-Data-START## Transcript exon combination :: BC148083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001094545.1
    CDS182..3088
    Translation

    Target ORF information:

    RefSeq Version NM_001101075.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase 1 (AARS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001101075.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_005218342.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218342.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218399.1
    CDS133..3039
    Translation

    Target ORF information:

    RefSeq Version XM_005218342.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218342.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_015475667.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331153.1
    CDS331..3237
    Translation

    Target ORF information:

    RefSeq Version XM_015475667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_005218342.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218342.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218399.1
    CDS168..3074
    Translation

    Target ORF information:

    RefSeq Version XM_005218342.4
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218342.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_005218341.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218398.1
    CDS150..3056
    Translation

    Target ORF information:

    RefSeq Version XM_005218341.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218341.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_010814304.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812606.1
    CDS46..2952
    Translation

    Target ORF information:

    RefSeq Version XM_010814304.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814304.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_010814304.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812606.1
    CDS44..2950
    Translation

    Target ORF information:

    RefSeq Version XM_010814304.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814304.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_015475667.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331153.1
    CDS209..3115
    Translation

    Target ORF information:

    RefSeq Version XM_015475667.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475667.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_005218343.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218400.1
    CDS228..3134
    Translation

    Target ORF information:

    RefSeq Version XM_005218343.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218343.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28249
    Clone ID Related Accession (Same CDS sequence) NM_001101075.2 , XM_005218342.3 , XM_015475667.1 , XM_005218342.4 , XM_005218341.2 , XM_010814304.3 , XM_010814304.2 , XM_015475667.2 , XM_005218343.2 , XM_005218343.3
    Accession Version XM_005218343.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alanine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218400.1
    CDS220..3126
    Translation

    Target ORF information:

    RefSeq Version XM_005218343.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218343.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACTCAA CCTTAACAGC AGGTGAAATC CGGCAGCGTT TTATAGATTT CTTCAAGAGA 
    AATGAACACA CCTATGTTCA CTCATCTGCC ACCATCCCTT TGGACGATCC CACTTTGCTC
    TTTGCCAACG CAGGCATGAA CCAGTTCAAA CCCATTTTTC TGAATACTAT TGACCCATCT
    CACCCCATGG CAAAGCTGAG CAGAGCTGCC AACACTCAGA AGTGCATCCG GGCTGGCGGC
    AAACACAATG ACCTGGATGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAGATG
    CTGGGCTCCT GGTCCTTTGG AGATTACTTC AAGGAACTGG CATGTAAGAT GGCTCTGGAA
    CTTCTTACCC AAGAGTTTGG CATTCCAGTT GAAAGACTCT ACGTTACTTA CTTTGGTGGG
    GATGAAGCAG CTGGCTTAGA ACCAGACCTG GAGTGCAAAC AGATTTGGCA AAACTTGGGG
    CTAGATGACA GCAGAATCCT CCCCGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC
    ACGGGCCCAT GTGGTCCCTG CAGTGAGATC CACTACGACC GCATCGGTGG TCGGGATGCA
    GCACACCTCG TCAACCAGGA CGACCCCAAC GTGCTGGAGA TCTGGAACCT TGTGTTCATC
    CAGTATAACA GGGAAACCGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA
    GGGATGGGCC TGGAGCGACT GGTGTCCGTG CTGCAGAATA AGATGTCCAA CTATGACACT
    GACCTCTTTG TCCCTTATTT TGAAGCCATC CAGAAGGGCA CAGGTGCTCG GCCGTATACT
    GGGAGAGTGG GTGCAGAGGA TGCTGACGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC
    CACGCTCGGA CCATCACTGT GGCCCTGGCC GATGGCGGCC GACCTGACAA TACGGGGCGG
    GGGTATGTGT TGAGACGGAT TCTCCGCCGG GCTGTTCGAT ACTCTCACGA GAAACTCAAC
    GCCAGCAGGG GTTTCTTTGC TACGTTAGTA GACGTTGTTG TCCAGTCTCT GGGAGATGCG
    TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAAGTG
    CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG
    GGAGACAGCA AAACCATTCC TGGGGATACC GCTTGGCTCC TCTATGACAC CTACGGGTTC
    CCCGTGGATC TCACTGCACT GATTGCTGAA GAGAAGGGGC TGGTGGTGGA CATGGATGGC
    TTTGACGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAG
    GACCTCATTA TGCTGGACAT TTATGCTATT GAAGAGCTCC GGGAAAAGGG TCTGGAGGCA
    ACAGATGATT CCCCGAAGTA CAGTTACCAT TCGGATTCCA GTGGAACCTA CGTGTTTGAG
    AGCGCAGTGG CTACAGTGAT GGCTCTGCGC CGGGACAGGA TGTTCGTGGA GGAAGCGTCC
    ACGGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTACGCCGA GCAGGGAGGG
    CAGACCTACG ACGAAGGGTA CCTGGTGAAG GTCGACGACA GCAGCGAAGA CAAAACTGAG
    TTCACAGTGA AGAACACTCA GGTCCGAGGA GGGTACGTGC TGCACATAGG AACGCTCTAC
    GGTAGCCTGA AAGTGGGAGA CCAGGTCCGG CTGTTCATTG ATGAGCCCCG GCGAAGGCCT
    GTCATGAGCA ACCACACAGC CACTCACATC CTGAACTTTG CCCTGCGCTC TGTGCTGGGG
    GAGGCTGACC AGAGAGGCTC CCTAGTTGCG CCTGACCGCC TTCGCTTTGA CTTCACTGCC
    AAGGGAGCCA TGTCCACCCA GCAGATCAAG AAGGCTGAGG AGATCGCAAA TGAGATGATT
    GAGGCAGCCA AGCCGGTCTA TACTGAGGAT TGTTCTCTGG CGGCAGCTAA AGCCATCCAG
    GGCCTGCGGG CTGTGTTTGA TGAGACCTAC CCTGACCCTG TGCGAGTCGT CTCCATTGGG
    GTCCCAGTGT CTGAGCTACT GGATGACCCC TCCGGTCCTG CTGGCTCGCT CACTTCTGTT
    GAGTTCTGTG GGGGAACACA CCTGCAGAAT TCGAGTCACG CGGGAGCGTT CGTGATCGTG
    AGTGAAGAAG CTATTGCCAA GGGCATCCGG AGGATAGTTG CTGTCACAGG GGCTGAAGCC
    CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATCTC TCTGTGTCGT GGAAGCCAAA
    GTGAAGGCCC AGGCCGCGCC GAACAAGGAC GTGCAGAGAG AGATCGCGGA CCTCGGGGAG
    GCCTTGGCCA CTGCCATCAT CCCTCAGTGG CAGAAGGATG AATTCCGGGA GAATCTCAAG
    TCTCTGAAGA AGGTCATGGA TGACCTAGAC CGGGCTAGCA AAGCCGATGT GCAGAAGCGG
    GTGTTGGAGA AGACGAAGCA GCTCATCGAC AGCAGCCCCA ACCAGCCCCT GGTCATCCTG
    GAGGTGGAGA GCGGCGCCTC GGCCAAGGCC CTGAACGAAG CCTTGAAGCT CTTTAAGATG
    CATTCCCCCC AGACGTCCGC CATGCTCTTC ACAGTGGATA ACGAGGCCGG CAGGATCACA
    TGCTTGTGTC AAGTCCCCCA GAATGCAGCC AACCGGGGCC TGAAGGCCAG TGAGTGGGTG
    CAGCAGGTGT CAGGCCTGAT GGATGGCAAA GGCGGTGGCA AAGACGTGTC TGCTCAGGCC
    ACGGGCAAGA ACGTGGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACCTC CTTTGCCCAG
    CTGCGCCTGG GAGACGTGAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.