CYCS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYCS
Entrez Gene ID	510767
Full Name	cytochrome c, somatic
Synonyms	cytc
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010804321.2	XP_010802623.1	cytochrome c isoform X1
XM_010804321.3	XP_010802623.1	cytochrome c isoform X1
NM_001046061.2	NP_001039526.1	cytochrome c

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CYCS	NP_001039526.1
Rattus norvegicus (Norway rat)	Cycs	NP_036971.1
Drosophila melanogaster (fruit fly)	Cyt-c-p	NP_477176.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009537	XP_310154.1
Eremothecium gossypii	AGOS_AFR360W	NP_985907.1
Rattus norvegicus (Norway rat)	LOC100363502	XP_003751847.1
Gallus gallus (chicken)	CYCS	NP_001072946.1
Homo sapiens (human)	CYCS	NP_061820.1
Caenorhabditis elegans (roundworm)	cyc-2.2	NP_506156.1
Kluyveromyces lactis	KLLA0F16929g	XP_455841.1
Xenopus tropicalis (tropical clawed frog)	cycs	NP_988895.1
	LOC694977	XP_001085135.1
Canis lupus familiaris (dog)	CYCS	NP_001183974.1
Mus musculus (house mouse)	Cycs	NP_031834.1
Caenorhabditis elegans (roundworm)	cyc-2.1	NP_500629.1
Saccharomyces cerevisiae (baker's yeast)	CYC1	NP_012582.1

Gene Ontology
Bibliography

Related articles in PubMed

Study on the apoptosis mediated by cytochrome c and factors that affect the activation of bovine longissimus muscle during postmortem aging.
Zhang J, Yu Q, Han L, Chen C, Li H, Han G
Apoptosis : an international journal on programmed cell death22(6)777-785(2017 Jun)

Ribose 5-phosphate glycation reduces cytochrome c respiratory activity and membrane affinity.
Hildick-Smith GJ, Downey MC, Gretebeck LM, Gersten RA, Sandwick RK
Biochemistry50(51)11047-57(2011 Dec)

Direct observation of vibrational energy flow in cytochrome c.
Fujii N, Mizuno M, Mizutani Y
The journal of physical chemistry. B115(44)13057-64(2011 Nov)

Evidence of protein collective motions on the picosecond timescale.
He Y, Chen JY, Knab JR, Zheng W, Markelz AG
Biophysical journal100(4)1058-65(2011 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data using ribose 5-P suggest that glycation of cyt c (at lysines in ATP binding site) results in cyto c being less able to transfer electrons to cytochrome oxidase and in reduced affinity of cyt c for cardiolipin-containing liposomes.
Title: Ribose 5-phosphate glycation reduces cytochrome c respiratory activity and membrane affinity.

It was found from temporal changes of the anti-Stokes Ultraviolet resonance Raman intensities that the energy flow from the heme to Trp59 and the energy release from Trp59 took place with the time constants of 1-3 and ~8 ps, respectively.
Title: Direct observation of vibrational energy flow in cytochrome c.

The presence of structural collective motions on a picosecond timescale for the heme protein, cytochrome c, as a function of oxidation and hydration, was investigated.
Title: Evidence of protein collective motions on the picosecond timescale.

peroxidase activity shown by cardiolipin-bound cytochrome c is indicative of a less packed protein tertiary conformation in the complex
Title: Insights into cytochrome c-cardiolipin interaction. Role played by ionic strength.

The following CYCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00218	NM_001046061.2 Latest version!	Bos taurus cytochrome c, somatic (CYCS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa00218	XM_010804321.3 Latest version!	Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa00218	XM_010804321.2	Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYCS ( NM_001046061.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039526.1 Bos taurus cytochrome c, somatic (CYCS), mRNA.

Price & Availability↑

CloneID	OBa00218
Clone ID Related Accession (Same CDS sequence)	XM_010804321.2 , XM_010804321.3 , NM_001046061.2
Accession Version	NM_001046061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	cytochrome c
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105397.1. On Aug 30, 2012 this sequence version replaced NM_001046061.1. ##Evidence-Data-START## Transcript exon combination :: BC105397.1, DV792068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039526.1
CDS	130..447
Translation	MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITWGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE

Target ORF information:

RefSeq Version	NM_001046061.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome c, somatic (CYCS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046061.2

ORF Insert Sequence:

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYCS ( XM_010804321.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802623.1 Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa00218
Clone ID Related Accession (Same CDS sequence)	XM_010804321.2 , XM_010804321.3 , NM_001046061.2
Accession Version	XM_010804321.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytochrome c isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802623.1
CDS	87..404
Translation	MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITWGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE

Target ORF information:

RefSeq Version	XM_010804321.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804321.2

ORF Insert Sequence:

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYCS ( XM_010804321.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802623.1 Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa00218
Clone ID Related Accession (Same CDS sequence)	XM_010804321.2 , XM_010804321.3 , NM_001046061.2
Accession Version	XM_010804321.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytochrome c isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802623.1
CDS	135..452
Translation	MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITWGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE

Target ORF information:

RefSeq Version	XM_010804321.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome c, somatic (CYCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804321.3

ORF Insert Sequence:

ATGGGTGATG TTGAGAAGGG CAAGAAGATT TTTGTTCAGA AGTGTGCCCA GTGCCATACT 
GTGGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAACCTCC ATGGTCTGTT TGGACGAAAG 
ACAGGTCAGG CTCCTGGATT CTCTTACACA GATGCCAATA AGAACAAAGG TATCACCTGG 
GGAGAGGAGA CGCTGATGGA GTACTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAG 
ATGATCTTTG CTGGCATTAA GAAGAAGGGA GAGAGGGAAG ACTTGATAGC TTATCTCAAA 
AAAGCTACCA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Study on the apoptosis mediated by cytochrome c and factors that affect the activation of bovine longissimus muscle during postmortem aging.
Apoptosis : an international journal on programmed cell death22(6)777-785(2017 Jun)
Zhang J,Yu Q,Han L,Chen C,Li H,Han G

Ribose 5-phosphate glycation reduces cytochrome c respiratory activity and membrane affinity.
Biochemistry50(51)11047-57(2011 Dec)
Hildick-Smith GJ,Downey MC,Gretebeck LM,Gersten RA,Sandwick RK

Direct observation of vibrational energy flow in cytochrome c.
The journal of physical chemistry. B115(44)13057-64(2011 Nov)
Fujii N,Mizuno M,Mizutani Y

Evidence of protein collective motions on the picosecond timescale.
Biophysical journal100(4)1058-65(2011 Feb)
He Y,Chen JY,Knab JR,Zheng W,Markelz AG

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CYCS cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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